WO1996003527A3 - Diagnostic method and probe - Google Patents

Diagnostic method and probe Download PDF

Info

Publication number
WO1996003527A3
WO1996003527A3 PCT/GB1995/001721 GB9501721W WO9603527A3 WO 1996003527 A3 WO1996003527 A3 WO 1996003527A3 GB 9501721 W GB9501721 W GB 9501721W WO 9603527 A3 WO9603527 A3 WO 9603527A3
Authority
WO
WIPO (PCT)
Prior art keywords
exon
diagnostic method
introns
sequence
probe
Prior art date
Application number
PCT/GB1995/001721
Other languages
French (fr)
Other versions
WO1996003527A2 (en
Inventor
David Tarin
Yasuhiro Matsumura
Original Assignee
Isis Innovation
David Tarin
Yasuhiro Matsumura
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9414704A external-priority patent/GB9414704D0/en
Priority claimed from GB9420878A external-priority patent/GB9420878D0/en
Priority claimed from GBGB9509881.0A external-priority patent/GB9509881D0/en
Application filed by Isis Innovation, David Tarin, Yasuhiro Matsumura filed Critical Isis Innovation
Priority to JP8505569A priority Critical patent/JPH10504188A/en
Priority to EP95925936A priority patent/EP0771361A2/en
Publication of WO1996003527A2 publication Critical patent/WO1996003527A2/en
Publication of WO1996003527A3 publication Critical patent/WO1996003527A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70585—CD44
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

Introns and a new exon, Exon 9a, are formed as transcripts during uncontrolled expression of the CD44 gene in tumour cells. Novel probes comprise labelled oligonucleotides which hybridize to complementary strands synthesized on introns. A diagnostic method comprises hybridizing these probes with nucleic acids recovered from mammalian cells. Intron In-9 having a 474 bp sequence and Exon 9a having a 436 bp sequence.
PCT/GB1995/001721 1994-07-21 1995-07-21 Diagnostic method and probe WO1996003527A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP8505569A JPH10504188A (en) 1994-07-21 1995-07-21 Diagnostic methods and probes
EP95925936A EP0771361A2 (en) 1994-07-21 1995-07-21 Diagnostic method and probe

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB9414704A GB9414704D0 (en) 1994-07-21 1994-07-21 Diagnostic method and probe
GB9420878A GB9420878D0 (en) 1994-10-17 1994-10-17 Diagnostic method and probe
GBGB9509881.0A GB9509881D0 (en) 1994-07-21 1995-05-16 Diagnostic method & probe
GB9509881.0 1995-05-16
GB9420878.2 1995-05-16
GB9414704.8 1995-05-16

Publications (2)

Publication Number Publication Date
WO1996003527A2 WO1996003527A2 (en) 1996-02-08
WO1996003527A3 true WO1996003527A3 (en) 1996-03-28

Family

ID=27267290

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1995/001721 WO1996003527A2 (en) 1994-07-21 1995-07-21 Diagnostic method and probe

Country Status (4)

Country Link
EP (1) EP0771361A2 (en)
JP (1) JPH10504188A (en)
CA (1) CA2195404A1 (en)
WO (1) WO1996003527A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999004036A1 (en) * 1997-07-14 1999-01-28 Isis Innovation Limited Cd44 based cancer detection
JP2006526998A (en) * 2003-06-12 2006-11-30 コリア リサーチ インスティテュート オブ バイオサイエンス アンド バイオテクノロジー Gastric cancer and metastatic gastric cancer diagnostic kit

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002783A1 (en) * 1986-10-16 1988-04-21 Nordisk Insulinlaboratorium A process and an agent for detection of gene structures in humans having a great tendency to develop iddm
JPH02109982A (en) * 1988-10-18 1990-04-23 Teijin Ltd Gene fragment, probe and detection of chromosomal aberration
WO1991010734A1 (en) * 1990-01-12 1991-07-25 Hsc Research Development Corporation Introns and exons of the cystic fibrosis gene and mutations at various positions of the gene
EP0558257A1 (en) * 1992-02-24 1993-09-01 The Scripps Research Institute Gaucher's disease: detection of a new mutation in intron 2 of the glucocerebrosidase gene
WO1993022458A1 (en) * 1992-04-24 1993-11-11 The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services A predictive assay for suicidal behavior
WO1994002633A1 (en) * 1992-07-21 1994-02-03 Isis Innovation Limited Diagnostic method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002783A1 (en) * 1986-10-16 1988-04-21 Nordisk Insulinlaboratorium A process and an agent for detection of gene structures in humans having a great tendency to develop iddm
JPH02109982A (en) * 1988-10-18 1990-04-23 Teijin Ltd Gene fragment, probe and detection of chromosomal aberration
WO1991010734A1 (en) * 1990-01-12 1991-07-25 Hsc Research Development Corporation Introns and exons of the cystic fibrosis gene and mutations at various positions of the gene
EP0558257A1 (en) * 1992-02-24 1993-09-01 The Scripps Research Institute Gaucher's disease: detection of a new mutation in intron 2 of the glucocerebrosidase gene
WO1993022458A1 (en) * 1992-04-24 1993-11-11 The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services A predictive assay for suicidal behavior
WO1994002633A1 (en) * 1992-07-21 1994-02-03 Isis Innovation Limited Diagnostic method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 014, no. 318 *
TARIN D ET AL: "derranged activity of the CD44 gene and other loci as biomarkers for progression to metastatic stability", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 17g, pages 173 - 185 *

Also Published As

Publication number Publication date
WO1996003527A2 (en) 1996-02-08
CA2195404A1 (en) 1996-02-08
JPH10504188A (en) 1998-04-28
EP0771361A2 (en) 1997-05-07

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