WO1995003543A1 - Potentiometric biosensor and the method of its use - Google Patents
Potentiometric biosensor and the method of its use Download PDFInfo
- Publication number
- WO1995003543A1 WO1995003543A1 PCT/US1994/008236 US9408236W WO9503543A1 WO 1995003543 A1 WO1995003543 A1 WO 1995003543A1 US 9408236 W US9408236 W US 9408236W WO 9503543 A1 WO9503543 A1 WO 9503543A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- redox mediator
- indicating
- fluid sample
- analyte
- potential
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode
Definitions
- the invention relates to the assay of an analyte by a potentiometric biosensor.
- Potentiometric biosensors are known.
- Conover et al .. U.S. Patent No. 4,713,165 discloses a sensor having ion selective electrodes.
- the sensor includes three cells, each cell having a reference half-cell (including a reference electrode and a reference fluid) and a measuring half-cell.
- the half-cells are separated by an ion selective membrane, and each half-cell is connected to an adjacent measuring half-cell by a porous material that permits ionic flow.
- the measuring half-cell may further include a membrane which includes an enzyme, an enzyme substrate, or an antigen.
- a sample that contains an analyte of interest is added to a measuring half-cell.
- a reaction sequence is set up to generate some ion (for example, ammonium ion or hydronium ion) . Therefore, the activity of the ion in the measuring half-cell changes.
- the change in potential of the measuring half-cell versus the reference half-cell reflects the change in activity in the measuring half-cell.
- the change in potential in the sample-containing half-cell may be correlated to the amount of analyte in the sample.
- U.S. Patent No. 4,340,448 discloses a potentiometric device for the assay of an analyte.
- the device has a working electrode and a reference electrode.
- An oxidase enzyme is immobilized on the working electrode.
- the working electrode must be made of a material, such as platinum, capable of measuring potential as a function of the change in the concentration of hydrogen peroxide.
- a sample containing glucose may be added to the device, wherein the working electrode includes glucose oxidase (and may also include catalase) .
- the change in potential reflects the production of hydrogen peroxide, produced by the following reaction:
- the change in potential may be correlated to the concentration of glucose in the sample.
- the electrode from exterior to interior, comprises a sheath (cellulose sheath) including dextranase, a dialysis membrane (optional) , a sheath including ⁇ -glucosidase, a sheath including glucose oxidase, and a platinum redox electrode.
- the electrode is immersed into a sample, containing an unknown amount of dextran and a known added amount of ferrocyanide (an excess) .
- Dextran is hydrolyzed (by the enzymes in the various sheaths) to glucose.
- Glucose is oxidized to gluconic acid, and molecular oxygen is reduced to hydrogen peroxide.
- Ferrocyanide is oxidized by hydrogen peroxide and is used as an indicator of the peroxide generated. Potential changes as ferrocyanide is oxidized to ferricyanide. The change in potential is correlated to the concentration of dextran in the sample.
- the invention is a potentiometric biosensor, test strip, reagent, and a potentiometric method for detection or measurement of an analyte from a fluid sample .
- the biosensor includes indicating and reference electrodes, and a reagent, which overlays a portion of the surface of the indicating electrode.
- the reagent includes an enzyme and a redox mediator.
- redox mediator e.g., potassium ferricyanide
- E° Formal potential of the redox couple (the oxidized and reduced forms of the redox mediator)
- the above reaction may be monitored by observing the potential at the indicating electrode surface, which changes as the ratio of C° /C° , changes.
- the inventive potentiometric biosensor measures the change in potential of the system as a result of the chemical reaction of analyte/enzyme/mediator. That change in potential may be correlated to the detection or measurement of analyte in the fluid sample.
- the potentiometric biosensor may operate by utilizing a test strip that includes an electrically insulative base, indicating and reference electrodes supported on the base, and an overlay of insulating material, which includes a cutout portion that exposes surfaces of the indicating and reference electrodes . Overlaying the indicating electrode in the cutout portion is a reagent of known amount .
- the reagent generally includes an enzyme and redox mediator, and preferably includes a buffer, a dispersant and crystallization inhibitor, a thickener, and a surfactant .
- a potentiometer in electrical connection with the indicating and reference electrodes is used to measure changes in potential that occur at the indicating electrode surface in response to the enzyme, analyte, redox mediator reaction.
- a fluid sample containing the analyte of interest is added to the reagent, thereby forming a test sample.
- the ensuing reaction of enzyme, analyte, and redox mediator produces a measurable change in potential at the surface of the indicating electrode. This measurable change in potential may be correlated to the detection or measurement of the amount of analyte in the fluid sample.
- FIG. 1 is a top view of an embodiment of the test strip for use in the potentiometric biosensor.
- Fig. 2 is a cross-sectional view of the biosensor shown in Fig. 1 along lines 12-12.
- Fig. 3 is a top view of another embodiment of the test strip for use in the potentiometric biosensor. DETAILED DESCRIPTION OF THE INVENTION
- the potentiometric biosensor may be used to analyze analytes involved in the following general reaction scheme: enzyme analyte + redox mediator > analyte + redox mediator
- E° Formal potential of the redox couple (the oxidized and reduced forms of the redox mediator)
- C° , Concentration or activity of the reduced form of red the redox mediator at the indicating electrode surface
- the system potential changes as the reaction progresses because the redox mediator is being converted from its oxidized form to its reduced form in the above examples . While the redox mediator is being reduced, analyte is being oxidized.
- changes in system potential which directly result from the changing ratio C°ox/C°red,, may - ⁇ be correlated to the concentration of analyte in the fluid sample.
- the potentiometric biosensor utilizes this phenomenon to detect or measure the amount of analyte from a fluid sample.
- the potentiometric biosensor generally includes the following elements: a. an indicating electrode; b. a reference electrode; c. a reagent of known amount overlaying a portion of the surface of the indicating electrode and comprising an enzyme and a redox mediator, the enzyme being of sufficient type and in sufficient amount to catalyze a reaction involving the enzyme, the redox mediator, and an analyte from a fluid sample, the redox mediator being in sufficient amount such that the reaction involving the enzyme, the redox mediator, and the analyte produces a measurable change in potential, at the surface of the indicating electrode, that is substantially attributable to the change in proportions of the oxidized and reduced forms of the redox mediator; and d.
- the potentiometric biosensor could be utilized as a potentiometric sensor.
- the analyte to be measured could be a non biological analyte; therefore, a catalyst other than an enzyme could be used. Further, if the analyte would react directly with the redox mediator, then no catalyst would be required.
- a reference electrode such as a silver/silver chloride reference electrode, is required so that measurements of potential will be accurately made with respect to a fixed reference point.
- the indicating electrode may be made of any electrically conducting material, such as palladium, platinum, gold, silver, titanium, copper, and carbon. It is important that the reagent overlay a portion of the surface of the indicating electrode so that potential difference measurements may be made at the indicating electrode surface. It is also important that the reagent contain a known amount of redox mediator. Because analyte determinations are made based upon measurements of system potential, which is determined by
- Lactate Lactate Dehydrogenase Ferricyande Phenazine and Diaphorase Ethosulfate, or Phenazine Methosulfate
- Uric Acid Uricase Ferricyanide I some of the examples shown in Table I, at least one additional enzyme is used as a reaction catalyst. Also, some of the examples shown in Table I may utilize an additional mediator, which facilitates electron transfer to the oxidized form of the redox mediator. The additional mediator may be provided to the reagent in lesser amount than the oxidized form of the redox mediator.
- the amount of enzyme included in the reagent may vary depending upon the time desired for completion of the reaction involving enzyme, analyte, and redox mediator. In general, if more enzyme is added, then the shorter the time period for completion of the reaction. Other factors, such as pH and ionic strength, can also influence the kinetics of this reaction.
- the biosensor reagent includes a dispersant and crystallization inhibitor, such as AVICEL RC-591F, a blend of 88% microcrystalline cellulose and 12% carboxymethyl- cellulose (available from FMC Corp.) , and a thickener, such as NATROSOL-250 M (a microcrystalline hydroxyethylcellulose available from Aqualon) .
- a dispersant and crystallization inhibitor such as AVICEL RC-591F
- a blend of 88% microcrystalline cellulose and 12% carboxymethyl- cellulose available from FMC Corp.
- NATROSOL-250 M a microcrystalline hydroxyethylcellulose available from Aqualon
- Other preferred reagent components include a buffer and a surfactant.
- the buffer should be of sufficient type and in sufficient amount to provide and maintain a pH at which the enzyme catalyzes the reaction involving the enzyme, the redox mediator, and the analyte.
- the surfactant should be of sufficient type and in sufficient amount to aid wetting of the film by a fluid sample (such as blood) .
- the reagent could be incorporated in a test strip for use in the potentiometric biosensor.
- the test strip would include the following elements : a. a first electrical insulator; b. indicating and reference electrodes supported on the first electrical insulator; c. a second electrical insulator, overlaying the first electrical insulator and the electrodes and including a cutout portion that exposes surface areas of the indicating and reference electrodes; and d. a known amount of biosensor reagent overlaying the exposed surface of the indicating electrode in the cutout portion.
- FIG. 1 and 2 depict the following elements: first electrical insulator 1 , indicating electrode 2, reference electrode 5, second electrical insulator 2, reagent 2_, cutout portion ______ t and additional cutout portion 13.
- First electrical insulator ____, and second electrical insulator 2 may be made of an insulative plastic, such as vinyl polymer or polyimide plastic, or other useful electrically insulating material .
- Indicating electrode 3_ may be made of palladium, gold, copper, platinum, carbon, silver, titanium, or other useful electrically conducting material .
- Reference electrode _5_ may be a silver/silver chloride electrode, a saturated calomel electrode, or other suitable reference electrode. Further, electrodes 2_ and _H may be affixed to insulator 1 , and insulator 1. maybe affixed to insulator 2, by the use of hot melt adhesive.
- a protocol for making a reagent 2. that could be added to cutout portion ___1 and used for the analysis of glucose from a blood sample is as follows :
- Step 1- Prepare 1 liter (in a volumetric flask) of a buffer/NATROSOL mixture by adding 1.2000 grams (g) NATROSOL- 250 M to 0.740 molar (M) aqueous potassium phosphate buffer (including 80.062 g monobasic potassium phosphate and 26.423 g dibasic potassium phosphate) at pH 6.25. Allow the buffer/NATROSOL mixture to stir and swell for 3 hours.
- Step 2- Prepare an AVICEL mixture by stirring 14.000 g AVICEL RC-591 F and 504.7750 g water for 20 minutes.
- Step 3- Prepare a TRITON mixture by adding 0.5000 g TRITON X-100 surfactant to 514.6000 g of the buffer/NATROSOL mixture and stir for 15 minutes.
- Step 4- While stirring, add the total TRITON mixture dropwise with a dropwise addition funnel or buret to the total AVICEL mixture. Once addition is complete, continue stirring overnight.
- Step 5- To the mixture resulting from Step 4, add, while stirring, about 25 g potassium ferricyanide. (Add a little potassium ferricyanide at a time to allow the potassium ferricyanide to dissolve as added.)
- Step 6- Stir the resulting mixture of Step 5 for 20 minutes .
- Step 7- Adjust the pH of the mixture resulting from Step 6 to 6.25 by adding potassium hydroxide.
- Step 8- To the resulting mixture of Step 7 add 9.1533 g glucose oxidase (218.50 units per milligram (mg) from Biozyme) and stir at least 20 minutes.
- Step 9- To the resulting mixture of Step 8 add 6 g disodium succinate and stir at least 20 minutes.
- Step 10- Filter the resulting mixture of Step 9 through a 100- micron sieve bag to remove AVICEL clumping.
- the filtrate is the resulting reagent composition, which is added to at least the indicating electrode surface in cutout portion 11 and is then dried.
- reagent made by the above-stated protocol should be added to at least the indicating electrode surface in cutout portion 11.
- This amount of reagent will contain a sufficient amount of ferricyanide, and a sufficient amount of enzyme (glucose oxidase) to catalyze the oxidation of glucose (from a sample of human whole blood) and the reduction of ferricyanide to completion within about 20 seconds.
- Reagent 2_ is then dried by heating at about 50' C for about 3 minutes. After drying, a polyester or nylon mesh may be placed on top of the dried reagent to aid in preventing loss of reagent from the biosensor during shipping and handling and to aid in minimizing human contamination from reagent 2.. The mesh may be affixed to substrate 7. by adhesive tape.
- a palladium indicating electrode and a silver/silver chloride reference electrode both in electrical connection with a potentiometer, were exposed to solutions of varying ferricyanide/ferrocyanide ratios.
- varying the ratio of ferricyanide/ferrocyanide resulted in a change in potential at the indicating electrode surface.
- the detection or measurement of an analyte from a fluid sample could be conducted by employing the following steps : a. forming a test sample by adding the fluid sample (e.g. , a blood sample) to the biosensor, wherein the reagent is fully exposed to the fluid sample and fluid sample contacts both the indicating and reference electrodes, thereby forming a circuit; b. incubating the test sample a sufficient period of time to produce a measurable change in potential, which is substantially attributable to the change in proportions of the oxidized and reduced forms of the redox mediator, at the surface of the indicating electrode. (The incubation period may be fixed at some period of time greater than the minimum time required to produce a measurable change in potential . ) ; c. measuring potential after the incubation period; and d. correlating the measured potential to the detection or measurement of the analyte from the fluid sample.
- a test sample by adding the fluid sample (e.g. , a blood sample) to the biosensor,
- a 20 ⁇ 1 sample of human whole blood may be added to cutout portion 11. thereby combining with reagent 2. and forming the test sample.
- the sample volume of human whole blood must be sufficient to wet reagent _£ and to connect electrodes 2 and 5. in cutout 1 ⁇ .
- the test sample may be incubated at ambient room temperature for about 20 seconds, or some other fixed period of time equal to or greater than the minimum time required to produce a measurable change in potential at the surface of the . indicating electrode, and potential at the surface of the indicating electrode (relative to the reference electrode) measured.
- the measured potential is then correlated to the amount of glucose (or the detection of glucose) in the blood sample by comparing like measurements with glucose solutions of known concentration.
- the potentiometric biosensor described above has several advantages. First, the potentiometric biosensor would be very inexpensive, requiring only a test strip like the one described above and an inexpensive potentiometer. Second, the potentiometric biosensor would be subject to fewer interferences than an amperometric biosensor. For example, in an amperometric biosensor, unwanted electroactive species will contribute to the measured current, thereby causing error in the assay. However, in the potentiometric biosensor, potential changes only as a result of the changing ratios of oxidized and reduced forms of the redox mediator.
- FIG. 3 illustrates a three electrode potentiometric biosensor strip, which includes first electrical insulator 22. , first indicating electrode 23. second indicating electrode 23., reference electrode 22, second electrical insulator 2_i, first reagent 22., second reagent 32 , cutout portion Jil, and additional cutout portion ____H .
- first reagent 22. could be the reagent described above for detecting or measuring glucose in a fluid sample
- reagent 2.2. could be a reagent for detecting or measuring one of the other analytes listed in Table 1.
- assays have been illustrated, wherein the analyte is oxidized and the redox mediator is reduced in the assay.
- the potentiometric biosensor also applies to assays wherein the analyte is enzymatically reduced and the redox mediator is oxidized.
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP94922667A EP0710358B1 (en) | 1993-07-23 | 1994-07-21 | Potentiometric biosensor and the method of its use |
CA002167822A CA2167822C (en) | 1993-07-23 | 1994-07-21 | Potentiometric biosensor and the method for its use |
DE69427353T DE69427353T2 (en) | 1993-07-23 | 1994-07-21 | POTENTIOMETRIC BIOSENSOR AND METHOD FOR USE THEREOF |
JP50531095A JP3387926B2 (en) | 1993-07-23 | 1994-07-21 | Potentiometric biosensor and method of using the same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/097,331 | 1993-07-23 | ||
US08/097,331 US5413690A (en) | 1993-07-23 | 1993-07-23 | Potentiometric biosensor and the method of its use |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1995003543A1 true WO1995003543A1 (en) | 1995-02-02 |
Family
ID=22262812
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1994/008236 WO1995003543A1 (en) | 1993-07-23 | 1994-07-21 | Potentiometric biosensor and the method of its use |
Country Status (7)
Country | Link |
---|---|
US (1) | US5413690A (en) |
EP (1) | EP0710358B1 (en) |
JP (1) | JP3387926B2 (en) |
CA (1) | CA2167822C (en) |
DE (1) | DE69427353T2 (en) |
ES (1) | ES2158900T3 (en) |
WO (1) | WO1995003543A1 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19957826C1 (en) * | 1999-11-25 | 2001-06-21 | Ufz Leipzighalle Gmbh | Stable biosensor production, comprises coating a redox mediator layer with an adhesive hydrogel gelled at low temperature |
WO2001073419A1 (en) * | 2000-03-29 | 2001-10-04 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
WO2009015291A1 (en) * | 2007-07-26 | 2009-01-29 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
US7960113B2 (en) | 2007-03-23 | 2011-06-14 | Hitachi, Ltd. | DNA analysis method and DNA analyzer |
US8128796B2 (en) | 2007-06-22 | 2012-03-06 | Hitachi, Limited | Analyzer |
US8202409B2 (en) | 2006-11-21 | 2012-06-19 | Hitachi, Ltd. | Potentiometric sensor and analytical element |
AU2013204779B2 (en) * | 2007-07-26 | 2015-05-28 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
US10374248B2 (en) | 2012-08-15 | 2019-08-06 | Lockheed Martin Energy, Llc | High solubility iron hexacyanides |
Families Citing this family (198)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPM506894A0 (en) * | 1994-04-14 | 1994-05-05 | Memtec Limited | Novel electrochemical cells |
GB9416002D0 (en) * | 1994-08-08 | 1994-09-28 | Univ Cranfield | Fluid transport device |
AUPN239395A0 (en) * | 1995-04-12 | 1995-05-11 | Memtec Limited | Method of defining an electrode area |
AUPN363995A0 (en) | 1995-06-19 | 1995-07-13 | Memtec Limited | Electrochemical cell |
US6413410B1 (en) * | 1996-06-19 | 2002-07-02 | Lifescan, Inc. | Electrochemical cell |
AUPN661995A0 (en) | 1995-11-16 | 1995-12-07 | Memtec America Corporation | Electrochemical cell 2 |
US6638415B1 (en) | 1995-11-16 | 2003-10-28 | Lifescan, Inc. | Antioxidant sensor |
US6863801B2 (en) * | 1995-11-16 | 2005-03-08 | Lifescan, Inc. | Electrochemical cell |
US6521110B1 (en) | 1995-11-16 | 2003-02-18 | Lifescan, Inc. | Electrochemical cell |
US5795453A (en) * | 1996-01-23 | 1998-08-18 | Gilmartin; Markas A. T. | Electrodes and metallo isoindole ringed compounds |
US5830341A (en) * | 1996-01-23 | 1998-11-03 | Gilmartin; Markas A. T. | Electrodes and metallo isoindole ringed compounds |
US5962215A (en) * | 1996-04-05 | 1999-10-05 | Mercury Diagnostics, Inc. | Methods for testing the concentration of an analyte in a body fluid |
DE19781229C2 (en) * | 1996-06-17 | 2002-02-28 | Mercury Diagnostics Inc | Electrochemical test device and method for its production |
US6632349B1 (en) | 1996-11-15 | 2003-10-14 | Lifescan, Inc. | Hemoglobin sensor |
US6379914B1 (en) | 1996-11-26 | 2002-04-30 | Lincoln Ventors Limited | Method and apparatus for measuring use of a substrate in a microbially catalyzed reaction |
US6071249A (en) | 1996-12-06 | 2000-06-06 | Abbott Laboratories | Method and apparatus for obtaining blood for diagnostic tests |
ATE227844T1 (en) | 1997-02-06 | 2002-11-15 | Therasense Inc | SMALL VOLUME SENSOR FOR IN-VITRO DETERMINATION |
AUPO581397A0 (en) * | 1997-03-21 | 1997-04-17 | Memtec America Corporation | Sensor connection means |
AUPO585797A0 (en) | 1997-03-25 | 1997-04-24 | Memtec America Corporation | Improved electrochemical cell |
JP3859239B2 (en) * | 1997-07-22 | 2006-12-20 | アークレイ株式会社 | Concentration measuring device, test piece for the concentration measuring device, biosensor system, and terminal forming method for the test piece |
AUPO855897A0 (en) | 1997-08-13 | 1997-09-04 | Usf Filtration And Separations Group Inc. | Automatic analysing apparatus II |
US6193865B1 (en) | 1997-09-11 | 2001-02-27 | Usf Filtration And Separations Group, Inc. | Analytic cell |
US5906921A (en) * | 1997-09-29 | 1999-05-25 | Matsushita Electric Industrial Co., Ltd. | Biosensor and method for quantitative measurement of a substrate using the same |
US6001239A (en) * | 1998-09-30 | 1999-12-14 | Mercury Diagnostics, Inc. | Membrane based electrochemical test device and related methods |
US5997817A (en) * | 1997-12-05 | 1999-12-07 | Roche Diagnostics Corporation | Electrochemical biosensor test strip |
US8071384B2 (en) | 1997-12-22 | 2011-12-06 | Roche Diagnostics Operations, Inc. | Control and calibration solutions and methods for their use |
US6878251B2 (en) * | 1998-03-12 | 2005-04-12 | Lifescan, Inc. | Heated electrochemical cell |
US6475360B1 (en) | 1998-03-12 | 2002-11-05 | Lifescan, Inc. | Heated electrochemical cell |
US6652734B1 (en) | 1999-03-16 | 2003-11-25 | Lifescan, Inc. | Sensor with improved shelf life |
US8688188B2 (en) | 1998-04-30 | 2014-04-01 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
US6949816B2 (en) | 2003-04-21 | 2005-09-27 | Motorola, Inc. | Semiconductor component having first surface area for electrically coupling to a semiconductor chip and second surface area for electrically coupling to a substrate, and method of manufacturing same |
US8480580B2 (en) | 1998-04-30 | 2013-07-09 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
US8346337B2 (en) | 1998-04-30 | 2013-01-01 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
US8465425B2 (en) | 1998-04-30 | 2013-06-18 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
US9066695B2 (en) | 1998-04-30 | 2015-06-30 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
US6175752B1 (en) | 1998-04-30 | 2001-01-16 | Therasense, Inc. | Analyte monitoring device and methods of use |
US8974386B2 (en) | 1998-04-30 | 2015-03-10 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
US6251260B1 (en) | 1998-08-24 | 2001-06-26 | Therasense, Inc. | Potentiometric sensors for analytic determination |
US6338790B1 (en) * | 1998-10-08 | 2002-01-15 | Therasense, Inc. | Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator |
US6591125B1 (en) | 2000-06-27 | 2003-07-08 | Therasense, Inc. | Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator |
EP1192269A2 (en) | 1999-06-18 | 2002-04-03 | Therasense, Inc. | MASS TRANSPORT LIMITED i IN VIVO /i ANALYTE SENSOR |
US7045054B1 (en) | 1999-09-20 | 2006-05-16 | Roche Diagnostics Corporation | Small volume biosensor for continuous analyte monitoring |
US6767440B1 (en) * | 2001-04-24 | 2004-07-27 | Roche Diagnostics Corporation | Biosensor |
US20050103624A1 (en) | 1999-10-04 | 2005-05-19 | Bhullar Raghbir S. | Biosensor and method of making |
US6645359B1 (en) | 2000-10-06 | 2003-11-11 | Roche Diagnostics Corporation | Biosensor |
US7073246B2 (en) | 1999-10-04 | 2006-07-11 | Roche Diagnostics Operations, Inc. | Method of making a biosensor |
US6662439B1 (en) | 1999-10-04 | 2003-12-16 | Roche Diagnostics Corporation | Laser defined features for patterned laminates and electrodes |
US6616819B1 (en) | 1999-11-04 | 2003-09-09 | Therasense, Inc. | Small volume in vitro analyte sensor and methods |
WO2001038873A2 (en) * | 1999-11-24 | 2001-05-31 | Biotronic Technologies, Inc. | Devices and methods for detecting analytes using electrosensor having capture reagent |
US6413395B1 (en) | 1999-12-16 | 2002-07-02 | Roche Diagnostics Corporation | Biosensor apparatus |
USD435300S (en) * | 1999-12-16 | 2000-12-19 | Roche Diagnostics Corporation | Biosensor |
US6780296B1 (en) | 1999-12-23 | 2004-08-24 | Roche Diagnostics Corporation | Thermally conductive sensor |
US6571651B1 (en) * | 2000-03-27 | 2003-06-03 | Lifescan, Inc. | Method of preventing short sampling of a capillary or wicking fill device |
US6612111B1 (en) | 2000-03-27 | 2003-09-02 | Lifescan, Inc. | Method and device for sampling and analyzing interstitial fluid and whole blood samples |
US6428664B1 (en) | 2000-06-19 | 2002-08-06 | Roche Diagnostics Corporation | Biosensor |
RU2278612C2 (en) * | 2000-07-14 | 2006-06-27 | Лайфскен, Инк. | Immune sensor |
US6444115B1 (en) | 2000-07-14 | 2002-09-03 | Lifescan, Inc. | Electrochemical method for measuring chemical reaction rates |
US6488828B1 (en) * | 2000-07-20 | 2002-12-03 | Roche Diagnostics Corporation | Recloseable biosensor |
US6540890B1 (en) * | 2000-11-01 | 2003-04-01 | Roche Diagnostics Corporation | Biosensor |
US6814843B1 (en) | 2000-11-01 | 2004-11-09 | Roche Diagnostics Corporation | Biosensor |
US6447657B1 (en) | 2000-12-04 | 2002-09-10 | Roche Diagnostics Corporation | Biosensor |
US6560471B1 (en) | 2001-01-02 | 2003-05-06 | Therasense, Inc. | Analyte monitoring device and methods of use |
EP1397068A2 (en) | 2001-04-02 | 2004-03-17 | Therasense, Inc. | Blood glucose tracking apparatus and methods |
US7473398B2 (en) | 2001-05-25 | 2009-01-06 | Roche Diagnostics Operations, Inc. | Biosensor |
DE10133363A1 (en) * | 2001-07-10 | 2003-01-30 | Infineon Technologies Ag | Measuring cell and measuring field with such measuring cells as well as using a measuring cell and using a measuring field |
WO2003062783A2 (en) * | 2001-07-20 | 2003-07-31 | North Carolina State University | Light addressable electrochemical detection of duplex structures |
US20030022150A1 (en) * | 2001-07-24 | 2003-01-30 | Sampson Jeffrey R. | Methods for detecting a target molecule |
US6814844B2 (en) | 2001-08-29 | 2004-11-09 | Roche Diagnostics Corporation | Biosensor with code pattern |
US6755949B1 (en) | 2001-10-09 | 2004-06-29 | Roche Diagnostics Corporation | Biosensor |
RU2297696C2 (en) * | 2001-10-10 | 2007-04-20 | Лайфскен, Инк. | Electrochemical cell |
US6997343B2 (en) * | 2001-11-14 | 2006-02-14 | Hypoguard Limited | Sensor dispensing device |
US6872299B2 (en) * | 2001-12-10 | 2005-03-29 | Lifescan, Inc. | Passive sample detection to initiate timing of an assay |
US20030111357A1 (en) * | 2001-12-13 | 2003-06-19 | Black Murdo M. | Test meter calibration |
DE10211540A1 (en) * | 2002-03-15 | 2003-10-16 | Kist Europe Forschungsges Mbh | Electrode for enzyme immunoassays has a flat polymElectrode for enzyme immunoassays has a flat polymer substrate, covered with a thin electrode elemener substrate, covered with a thin electrode element layer in a low cost unit with a long life t layer in a low cost unit with a long life |
US20030180814A1 (en) * | 2002-03-21 | 2003-09-25 | Alastair Hodges | Direct immunosensor assay |
US6866758B2 (en) * | 2002-03-21 | 2005-03-15 | Roche Diagnostics Corporation | Biosensor |
US20060134713A1 (en) | 2002-03-21 | 2006-06-22 | Lifescan, Inc. | Biosensor apparatus and methods of use |
GB0211449D0 (en) * | 2002-05-17 | 2002-06-26 | Oxford Biosensors Ltd | Analyte measurement |
US7250095B2 (en) * | 2002-07-11 | 2007-07-31 | Hypoguard Limited | Enzyme electrodes and method of manufacture |
AU2003296186A1 (en) | 2002-12-24 | 2004-07-22 | Ikeda Food Research Co., Ltd. | Coenzyme-binding glucose dehydrogenase |
AU2003303597A1 (en) | 2002-12-31 | 2004-07-29 | Therasense, Inc. | Continuous glucose monitoring system and methods of use |
US7264139B2 (en) * | 2003-01-14 | 2007-09-04 | Hypoguard Limited | Sensor dispensing device |
US20040180369A1 (en) * | 2003-01-16 | 2004-09-16 | North Carolina State University | Photothermal detection of nucleic acid hybridization |
US7587287B2 (en) | 2003-04-04 | 2009-09-08 | Abbott Diabetes Care Inc. | Method and system for transferring analyte test data |
US8066639B2 (en) | 2003-06-10 | 2011-11-29 | Abbott Diabetes Care Inc. | Glucose measuring device for use in personal area network |
US7645373B2 (en) | 2003-06-20 | 2010-01-12 | Roche Diagnostic Operations, Inc. | System and method for coding information on a biosensor test strip |
US8071030B2 (en) | 2003-06-20 | 2011-12-06 | Roche Diagnostics Operations, Inc. | Test strip with flared sample receiving chamber |
US7718439B2 (en) * | 2003-06-20 | 2010-05-18 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
US7452457B2 (en) | 2003-06-20 | 2008-11-18 | Roche Diagnostics Operations, Inc. | System and method for analyte measurement using dose sufficiency electrodes |
US8206565B2 (en) * | 2003-06-20 | 2012-06-26 | Roche Diagnostics Operation, Inc. | System and method for coding information on a biosensor test strip |
US8679853B2 (en) | 2003-06-20 | 2014-03-25 | Roche Diagnostics Operations, Inc. | Biosensor with laser-sealed capillary space and method of making |
US7488601B2 (en) * | 2003-06-20 | 2009-02-10 | Roche Diagnostic Operations, Inc. | System and method for determining an abused sensor during analyte measurement |
US7645421B2 (en) * | 2003-06-20 | 2010-01-12 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
US8148164B2 (en) | 2003-06-20 | 2012-04-03 | Roche Diagnostics Operations, Inc. | System and method for determining the concentration of an analyte in a sample fluid |
JP2007524816A (en) | 2003-06-20 | 2007-08-30 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | Method for producing thin uniform reagent strip and its reagent |
US8058077B2 (en) | 2003-06-20 | 2011-11-15 | Roche Diagnostics Operations, Inc. | Method for coding information on a biosensor test strip |
US7604721B2 (en) * | 2003-06-20 | 2009-10-20 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
WO2005067425A2 (en) * | 2003-07-10 | 2005-07-28 | Polytechnic University | Bacterial biosensors |
US20100000881A1 (en) * | 2003-10-30 | 2010-01-07 | North Carolina State University | Electrochemical detection of nucleic acid hybridization |
US20050150763A1 (en) * | 2004-01-09 | 2005-07-14 | Butters Colin W. | Biosensor and method of manufacture |
BRPI0507376A (en) | 2004-02-06 | 2007-07-10 | Bayer Healthcare Llc | oxidizable species as an internal reference for biosensors and method of use |
EP1718198A4 (en) | 2004-02-17 | 2008-06-04 | Therasense Inc | Method and system for providing data communication in continuous glucose monitoring and management system |
EP1728069A4 (en) * | 2004-03-17 | 2009-08-19 | Ca Nat Research Council | Method and apparatus for the detection of microorganisms |
US7569126B2 (en) | 2004-06-18 | 2009-08-04 | Roche Diagnostics Operations, Inc. | System and method for quality assurance of a biosensor test strip |
WO2007013872A2 (en) | 2004-07-22 | 2007-02-01 | The Board Of Trustees Of The University Of Illinois | Sensors employing single-walled carbon nanotubes |
AU2005295106B2 (en) | 2004-10-12 | 2012-03-15 | Bayer Healthcare Llc | Concentration determination in a diffusion barrier layer |
EP2365073A1 (en) | 2005-03-25 | 2011-09-14 | Ikeda Food Research Co. Ltd. | Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same |
US8112240B2 (en) | 2005-04-29 | 2012-02-07 | Abbott Diabetes Care Inc. | Method and apparatus for providing leak detection in data monitoring and management systems |
CN103558284B (en) | 2005-07-20 | 2017-04-12 | 安晟信医疗科技控股公司 | Gated amperometry |
WO2007035527A2 (en) | 2005-09-15 | 2007-03-29 | Duke University | Non-fouling polymeric surface modification and signal amplification method for biomolecular detection |
CN101273266B (en) | 2005-09-30 | 2012-08-22 | 拜尔健康护理有限责任公司 | Gated voltammetry |
US7766829B2 (en) | 2005-11-04 | 2010-08-03 | Abbott Diabetes Care Inc. | Method and system for providing basal profile modification in analyte monitoring and management systems |
WO2007056338A2 (en) | 2005-11-08 | 2007-05-18 | Surmodics, Inc. | Ultra-thin photo-polymeric coatings and uses thereof |
US7885698B2 (en) | 2006-02-28 | 2011-02-08 | Abbott Diabetes Care Inc. | Method and system for providing continuous calibration of implantable analyte sensors |
US8226891B2 (en) | 2006-03-31 | 2012-07-24 | Abbott Diabetes Care Inc. | Analyte monitoring devices and methods therefor |
US7620438B2 (en) | 2006-03-31 | 2009-11-17 | Abbott Diabetes Care Inc. | Method and system for powering an electronic device |
US8529751B2 (en) | 2006-03-31 | 2013-09-10 | Lifescan, Inc. | Systems and methods for discriminating control solution from a physiological sample |
US7920907B2 (en) | 2006-06-07 | 2011-04-05 | Abbott Diabetes Care Inc. | Analyte monitoring system and method |
US8732188B2 (en) | 2007-02-18 | 2014-05-20 | Abbott Diabetes Care Inc. | Method and system for providing contextual based medication dosage determination |
US8930203B2 (en) | 2007-02-18 | 2015-01-06 | Abbott Diabetes Care Inc. | Multi-function analyte test device and methods therefor |
US8123686B2 (en) | 2007-03-01 | 2012-02-28 | Abbott Diabetes Care Inc. | Method and apparatus for providing rolling data in communication systems |
US8461985B2 (en) | 2007-05-08 | 2013-06-11 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods |
US7928850B2 (en) | 2007-05-08 | 2011-04-19 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods |
US8665091B2 (en) | 2007-05-08 | 2014-03-04 | Abbott Diabetes Care Inc. | Method and device for determining elapsed sensor life |
US8456301B2 (en) | 2007-05-08 | 2013-06-04 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods |
US8778168B2 (en) * | 2007-09-28 | 2014-07-15 | Lifescan, Inc. | Systems and methods of discriminating control solution from a physiological sample |
ES2637381T3 (en) * | 2007-10-31 | 2017-10-13 | F. Hoffmann-La Roche Ag | Electrical standards for biosensors and manufacturing procedure |
WO2009076302A1 (en) | 2007-12-10 | 2009-06-18 | Bayer Healthcare Llc | Control markers for auto-detection of control solution and methods of use |
GB0725234D0 (en) * | 2007-12-24 | 2008-02-06 | Oxtex Ltd | Electrochemical assays |
US8513371B2 (en) * | 2007-12-31 | 2013-08-20 | Bridgestone Corporation | Amino alkoxy-modified silsesquioxanes and method of preparation |
US8603768B2 (en) | 2008-01-17 | 2013-12-10 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
US8796184B2 (en) | 2008-03-28 | 2014-08-05 | Sentilus, Inc. | Detection assay devices and methods of making and using the same |
US8551320B2 (en) | 2008-06-09 | 2013-10-08 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
US20100051455A1 (en) * | 2008-08-26 | 2010-03-04 | Roche Diagnostics Operations, Inc. | Biosensor test strip cards |
US20110174618A1 (en) * | 2008-09-30 | 2011-07-21 | Menai Medical Technologies Limited | Sample measurement system |
JP5139538B2 (en) | 2008-11-04 | 2013-02-06 | 株式会社日立製作所 | Potential difference sensor chip, potential difference measuring method, and measurement kit |
US8103456B2 (en) | 2009-01-29 | 2012-01-24 | Abbott Diabetes Care Inc. | Method and device for early signal attenuation detection using blood glucose measurements |
US20100213057A1 (en) | 2009-02-26 | 2010-08-26 | Benjamin Feldman | Self-Powered Analyte Sensor |
US9226701B2 (en) | 2009-04-28 | 2016-01-05 | Abbott Diabetes Care Inc. | Error detection in critical repeating data in a wireless sensor system |
WO2010138856A1 (en) | 2009-05-29 | 2010-12-02 | Abbott Diabetes Care Inc. | Medical device antenna systems having external antenna configurations |
WO2011026147A1 (en) | 2009-08-31 | 2011-03-03 | Abbott Diabetes Care Inc. | Analyte signal processing device and methods |
WO2011026148A1 (en) | 2009-08-31 | 2011-03-03 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods for managing power and noise |
US9320461B2 (en) | 2009-09-29 | 2016-04-26 | Abbott Diabetes Care Inc. | Method and apparatus for providing notification function in analyte monitoring systems |
JP5309042B2 (en) * | 2010-01-22 | 2013-10-09 | 株式会社日立ハイテクノロジーズ | Potential difference measuring apparatus and potential difference measuring method |
US20110186428A1 (en) | 2010-01-29 | 2011-08-04 | Roche Diagnostics Operations, Inc. | Electrode arrangements for biosensors |
GB201005357D0 (en) | 2010-03-30 | 2010-05-12 | Menai Medical Technologies Ltd | Sampling plate |
GB201005359D0 (en) | 2010-03-30 | 2010-05-12 | Menai Medical Technologies Ltd | Sampling plate |
EP3305192B1 (en) | 2010-05-17 | 2019-10-09 | Sentilus Holdco, LLC | Detection devices and related methods of use |
CA2838797C (en) | 2011-07-27 | 2020-03-10 | Agamatrix, Inc. | Dry reagent comprising tetramethylammonium ferricyanide for electrochemical test strips |
WO2013027359A1 (en) | 2011-08-19 | 2013-02-28 | 株式会社村田製作所 | Living organism sensor |
EP2573171B1 (en) | 2011-09-20 | 2015-04-15 | Roche Diagnostics GmbH | Mutant lactate oxidase with increased stability and product, methods and uses involving the same |
JP6443802B2 (en) | 2011-11-07 | 2018-12-26 | アボット ダイアベティス ケア インコーポレイテッドAbbott Diabetes Care Inc. | Analyte monitoring apparatus and method |
JP5610033B2 (en) | 2012-06-06 | 2014-10-22 | パナソニック株式会社 | Method for accurately quantifying chemical substances contained in a sample solution at a very low concentration of 1 × 10 −8 M or less |
EP2679156B1 (en) | 2012-06-28 | 2019-10-02 | Roche Diabetes Care GmbH | Device for monitoring at least one body function of a user and method for manufacturing the same |
US9968306B2 (en) | 2012-09-17 | 2018-05-15 | Abbott Diabetes Care Inc. | Methods and apparatuses for providing adverse condition notification with enhanced wireless communication range in analyte monitoring systems |
CN105247356B (en) | 2013-03-15 | 2017-11-07 | 豪夫迈·罗氏有限公司 | Use the method and the unit and system of merging methods described of the information of the recovery pulse in being measured from electrochemical analyte |
CA2900572C (en) | 2013-03-15 | 2018-02-13 | F. Hoffmann-La Roche Ag | Methods of detecting high antioxidant levels during electrochemical measurements and failsafing an analyte concentration therefrom as well as devices, apparatuses and systems incorporting the same |
US9121050B2 (en) | 2013-03-15 | 2015-09-01 | American Sterilizer Company | Non-enzyme based detection method for electronic monitoring of biological indicator |
US8858884B2 (en) | 2013-03-15 | 2014-10-14 | American Sterilizer Company | Coupled enzyme-based method for electronic monitoring of biological indicator |
US10041901B2 (en) | 2013-03-15 | 2018-08-07 | Roche Diabetes Care, Inc. | Electrode configuration for a biosensor |
KR101727447B1 (en) | 2013-03-15 | 2017-04-14 | 에프. 호프만-라 로슈 아게 | Methods of scaling data used to construct biosensor algorithms as well as devices, apparatuses and systems incorporating the same |
JP6412027B2 (en) | 2013-03-15 | 2018-10-24 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | Method for fail-safe electrochemical measurement of analyte and device, apparatus and system incorporating the same |
EP2840144A1 (en) | 2013-08-20 | 2015-02-25 | Roche Diagniostics GmbH | A method for making a dry sensor element for an enzymatic determination of an analyte in a body fluid, a dry sensor element and article |
EP2840143A1 (en) | 2013-08-20 | 2015-02-25 | Roche Diagniostics GmbH | A method for making a dry sensor element for an enzymatic determination of an analyte in a body fluid and a dry sensor element |
WO2016073395A1 (en) | 2014-11-03 | 2016-05-12 | Roche Diabetes Care, Inc. | Electrode arrangements for electrochemical test elements and methods of use thereof |
EP3325966B1 (en) | 2015-07-20 | 2021-01-20 | Sentilus Holdco, LLC | Chips, detectors, and methods of making and using the same |
EP3170453B1 (en) | 2015-11-19 | 2021-03-17 | Roche Diabetes Care GmbH | Sensor assembly for detecting at least one analyte in a body fluid and method of assembling a sensor assembly |
EP3170451A1 (en) | 2015-11-19 | 2017-05-24 | Roche Diabetes Care GmbH | Sensor and sensor assembly for detecting an analyte in a body fluid |
EP3170452B1 (en) | 2015-11-19 | 2021-01-27 | Roche Diabetes Care GmbH | Sensor assembly for detecting at least one analyte in a body fluid |
CN108350439A (en) | 2015-12-21 | 2018-07-31 | 豪夫迈·罗氏有限公司 | Mutant 3-hydroxybutyrate dehydrogenase and its correlation technique from Alcaligenes faecalis and purposes |
EP3195795B1 (en) | 2016-01-19 | 2023-08-23 | Roche Diabetes Care GmbH | Sensor assembly and method for detecting at least one analyte in a body fluid |
DK3199121T3 (en) | 2016-01-29 | 2018-12-10 | Hoffmann La Roche | FUNCTIONAL MEDICAL PACKAGING AND MEDICAL DEVICE FOR INSTALLING AT LEAST ONE SUB-SYSTEM IN A HOST |
RS64277B1 (en) | 2016-02-05 | 2023-07-31 | Hoffmann La Roche | Medical device for detecting at least one analyte in a body fluid |
DE202016009190U1 (en) | 2016-02-05 | 2023-08-23 | Roche Diabetes Care Gmbh | Medical device for detecting at least one analyte in a body fluid |
ES2886024T3 (en) | 2016-02-05 | 2021-12-16 | Hoffmann La Roche | Medical device for detecting at least one analyte in a body fluid |
DE202016009189U1 (en) | 2016-02-05 | 2023-08-25 | Roche Diabetes Care Gmbh | Medical device for detecting at least one analyte in a body fluid |
DE202016009188U1 (en) | 2016-02-05 | 2023-08-30 | Roche Diabetes Care Gmbh | Medical device for detecting at least one analyte in a body fluid |
CN108473966A (en) | 2016-02-09 | 2018-08-31 | 豪夫迈·罗氏有限公司 | Mutant 3-hydroxybutyrate dehydrogenase and its correlation technique from hydrogenlike silicon ion and purposes |
EP3220137B1 (en) | 2016-03-14 | 2019-01-23 | Roche Diabetes Care GmbH | Method for detecting an interferent contribution in a biosensor |
EP3242233B1 (en) | 2016-05-03 | 2022-05-04 | Roche Diabetes Care GmbH | Sensor device for detecting at least one analyte in a body fluid of a user |
CN109561833B (en) | 2016-06-22 | 2022-04-29 | 豪夫迈·罗氏有限公司 | Medical device for percutaneous insertion of an insertable element into body tissue |
EP3984451A1 (en) | 2016-06-29 | 2022-04-20 | Roche Diabetes Care GmbH | Method for providing a signal quality degree associated with an analyte value measured in a continuous monitoring system |
EP3278729B1 (en) | 2016-08-04 | 2020-06-24 | Roche Diabetes Care GmbH | Medical device for detecting at least one analyte in a body fluid |
JP2019529935A (en) | 2016-10-05 | 2019-10-17 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | Detection reagents and electrode arrangements for multi-sample diagnostic test elements and methods of using them |
EP3551760A1 (en) | 2016-12-08 | 2019-10-16 | Roche Diabetes Care GmbH | Sensor device for determining the concentration of an analyte underin-vivo |
EP3339431A1 (en) | 2016-12-22 | 2018-06-27 | Roche Diabetes Care GmbH | Glucose dehydrogenase variants with improved properties |
RU2738794C1 (en) | 2017-03-21 | 2020-12-16 | Ф. Хоффманн-Ля Рош Аг | Medical device and method for its production |
EP3406193B1 (en) | 2017-05-23 | 2021-12-08 | Roche Diabetes Care GmbH | Sensor system and method for manufacturing thereof |
ES2960443T3 (en) | 2017-07-03 | 2024-03-04 | Hoffmann La Roche | Method and electronic unit to detect in vivo properties of a biosensor |
EP3457121A1 (en) | 2017-09-18 | 2019-03-20 | Roche Diabetes Care GmbH | Electrochemical sensor and sensor system for detecting at least one analyte |
EP3700407A1 (en) | 2017-10-27 | 2020-09-02 | Roche Diabetes Care GmbH | A device and a method for detecting at least one analyte in a body fluid of a user |
CN110514704B (en) * | 2018-05-22 | 2024-02-13 | 爱科来株式会社 | Novel biosensing method |
EP3928697A1 (en) | 2020-06-23 | 2021-12-29 | Roche Diabetes Care GmbH | Analyte sensor and a method for producing an analyte sensor |
TW202233124A (en) | 2020-10-26 | 2022-09-01 | 瑞士商赫孚孟拉羅股份公司 | Analyte sensor system and a method for its producing |
EP4243690A1 (en) | 2020-11-12 | 2023-09-20 | F. Hoffmann-La Roche AG | Method for manufacturing at least one electrode of an analyte sensor |
EP4000514A1 (en) | 2020-11-12 | 2022-05-25 | Roche Diabetes Care GmbH | Method for producing an analyte sensor, an analyte sensor, and a use thereof |
EP4085829A1 (en) | 2021-05-06 | 2022-11-09 | Roche Diabetes Care GmbH | Analyte sensor and a method for its producing |
EP4137046A1 (en) | 2021-08-18 | 2023-02-22 | Roche Diabetes Care GmbH | Analyte sensor and a method for its producing |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4340448A (en) * | 1978-08-28 | 1982-07-20 | University Of Pittsburgh | Potentiometric detection of hydrogen peroxide and apparatus therefor |
US4844910A (en) * | 1986-12-02 | 1989-07-04 | Euroceltique, S.A. | Spheroids |
US5264103A (en) * | 1991-10-18 | 1993-11-23 | Matsushita Electric Industrial Co., Ltd. | Biosensor and a method for measuring a concentration of a substrate in a sample |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4552840A (en) * | 1982-12-02 | 1985-11-12 | California And Hawaiian Sugar Company | Enzyme electrode and method for dextran analysis |
GB8526902D0 (en) * | 1985-10-31 | 1985-12-04 | Unilever Plc | Electrochemical analysis |
US4713165A (en) * | 1986-07-02 | 1987-12-15 | Ilex Corporation | Sensor having ion-selective electrodes |
DE68924026T3 (en) * | 1988-03-31 | 2008-01-10 | Matsushita Electric Industrial Co., Ltd., Kadoma | BIOSENSOR AND ITS MANUFACTURE. |
WO1991009139A1 (en) * | 1989-12-15 | 1991-06-27 | Boehringer Mannheim Corporation | Redox mediator reagent and biosensor |
US5688768A (en) * | 1991-02-19 | 1997-11-18 | Cor Therapeutics, Inc. | Recombinant thrombin receptor and related pharmaceuticals |
WO1993018141A1 (en) * | 1992-03-02 | 1993-09-16 | Biogen, Inc. | Thrombin receptor antagonists |
-
1993
- 1993-07-23 US US08/097,331 patent/US5413690A/en not_active Expired - Lifetime
-
1994
- 1994-07-21 WO PCT/US1994/008236 patent/WO1995003543A1/en active IP Right Grant
- 1994-07-21 ES ES94922667T patent/ES2158900T3/en not_active Expired - Lifetime
- 1994-07-21 CA CA002167822A patent/CA2167822C/en not_active Expired - Fee Related
- 1994-07-21 DE DE69427353T patent/DE69427353T2/en not_active Expired - Lifetime
- 1994-07-21 JP JP50531095A patent/JP3387926B2/en not_active Expired - Fee Related
- 1994-07-21 EP EP94922667A patent/EP0710358B1/en not_active Expired - Lifetime
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4340448A (en) * | 1978-08-28 | 1982-07-20 | University Of Pittsburgh | Potentiometric detection of hydrogen peroxide and apparatus therefor |
US4844910A (en) * | 1986-12-02 | 1989-07-04 | Euroceltique, S.A. | Spheroids |
US5264103A (en) * | 1991-10-18 | 1993-11-23 | Matsushita Electric Industrial Co., Ltd. | Biosensor and a method for measuring a concentration of a substrate in a sample |
Non-Patent Citations (1)
Title |
---|
See also references of EP0710358A4 * |
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19957826C1 (en) * | 1999-11-25 | 2001-06-21 | Ufz Leipzighalle Gmbh | Stable biosensor production, comprises coating a redox mediator layer with an adhesive hydrogel gelled at low temperature |
US8673127B2 (en) | 2000-03-29 | 2014-03-18 | Panasonic Corporation | Biosensor |
WO2001073419A1 (en) * | 2000-03-29 | 2001-10-04 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
US6911131B2 (en) | 2000-03-29 | 2005-06-28 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
US7648617B2 (en) | 2000-03-29 | 2010-01-19 | Panasonic Corporation | Biosensor |
US8702933B2 (en) | 2006-11-21 | 2014-04-22 | Hitachi, Ltd. | Potentiometric sensor and analytical element |
US8202409B2 (en) | 2006-11-21 | 2012-06-19 | Hitachi, Ltd. | Potentiometric sensor and analytical element |
US8246810B2 (en) | 2007-03-23 | 2012-08-21 | Hitachi, Ltd. | DNA analysis method and DNA analyzer |
US7960113B2 (en) | 2007-03-23 | 2011-06-14 | Hitachi, Ltd. | DNA analysis method and DNA analyzer |
US8128796B2 (en) | 2007-06-22 | 2012-03-06 | Hitachi, Limited | Analyzer |
EP2584044A3 (en) * | 2007-07-26 | 2013-11-27 | Agamatrix, Inc. | Electrochemical analyte detections apparatus and method |
EP2174135A4 (en) * | 2007-07-26 | 2010-08-25 | Agamatrix Inc | Electrochemical analyte detection apparatus and method |
CN101802612A (en) * | 2007-07-26 | 2010-08-11 | 埃葛梅崔克斯股份有限公司 | Electrochemical analyte detection apparatus and method |
AU2008279042B2 (en) * | 2007-07-26 | 2014-03-13 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
EP2174135A1 (en) * | 2007-07-26 | 2010-04-14 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
WO2009015291A1 (en) * | 2007-07-26 | 2009-01-29 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
AU2013204779B2 (en) * | 2007-07-26 | 2015-05-28 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
KR101532064B1 (en) * | 2007-07-26 | 2015-06-26 | 아가매트릭스, 인코포레이티드 | Electrochemical analyte detection apparatus and method |
US9939440B2 (en) | 2007-07-26 | 2018-04-10 | Agamatrix, Inc. | Electrochemical analyte detection apparatus and method |
US10374248B2 (en) | 2012-08-15 | 2019-08-06 | Lockheed Martin Energy, Llc | High solubility iron hexacyanides |
US11349141B2 (en) | 2012-08-15 | 2022-05-31 | Lockheed Martin Energy, Llc | High solubility iron hexacyanides |
Also Published As
Publication number | Publication date |
---|---|
DE69427353D1 (en) | 2001-07-05 |
EP0710358B1 (en) | 2001-05-30 |
DE69427353T2 (en) | 2001-10-18 |
CA2167822C (en) | 2004-02-24 |
JP3387926B2 (en) | 2003-03-17 |
CA2167822A1 (en) | 1995-02-02 |
EP0710358A1 (en) | 1996-05-08 |
JPH09500727A (en) | 1997-01-21 |
ES2158900T3 (en) | 2001-09-16 |
US5413690A (en) | 1995-05-09 |
EP0710358A4 (en) | 1996-09-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0710358B1 (en) | Potentiometric biosensor and the method of its use | |
AU712527B2 (en) | Electrochemical biosensor test strip | |
US5508171A (en) | Assay method with enzyme electrode system | |
CA2069946C (en) | Redox mediator reagent and biosensor | |
Thevenot et al. | Enzyme collagen membrane for electrochemical determination of glucose | |
Dicks et al. | Mediated amperometric biosensors for D-galactose, glycolate and L-amino acids based on a ferrocene-modified carbon paste electrode | |
US5942102A (en) | Electrochemical method | |
US5705045A (en) | Multi-biosensor for GPT and got activity | |
US5108564A (en) | Method and apparatus for amperometric diagnostic analysis | |
FI101021B (en) | Method and apparatus for measuring the concentration of a compound in a sample | |
Crumbliss et al. | A carrageenan hydrogel stabilized colloidal gold multi-enzyme biosensor electrode utilizing immobilized horseradish peroxidase and cholesterol oxidase/cholesterol esterase to detect cholesterol in serum and whole blood | |
EP0048090A2 (en) | Substrate specific galactose oxidase enzyme electrodes | |
Pundir et al. | Construction of an amperometric triglyceride biosensor using PVA membrane bound enzymes | |
Hilditch et al. | Disposable electrochemical biosensors | |
US20010052472A1 (en) | Biosensor and method for quantitating biochemical substrate using the same | |
US6214612B1 (en) | Cholesterol sensor containing electrodes, cholesterol dehydrogenase, nicotinamide adenine dinucleotide and oxidized electron mediator | |
Huang et al. | Amperometric determination of total cholesterol in serum, with use of immobilized cholesterol ester hydrolase and cholesterol oxidase | |
Elekes et al. | Bi-enzyme reactor for electrochemical detection of low concentrations of uric acid and glucose | |
EP1612275B1 (en) | Methods and compositions for characterizing a redox reagent system enzyme | |
US4042462A (en) | Creatine phosphokinase determination method | |
Christiansen et al. | The slow penetration of enzymebased biosensors into clinical chemistry analysis | |
Hu et al. | A bienzyme sensor for the determination of hypoxanthine and inosine | |
JP2001242133A (en) | Disposable bun sensor and production method thereof | |
CA2499527A1 (en) | An analyzer for the simultaneous enzymatic detection of closely related analytes | |
Wang | Electrochemical transduction |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): CA JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2167822 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1994922667 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1994922667 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 1994922667 Country of ref document: EP |