TITLE "SYNTHETIC BLOWFLY ATTRACTANT" FIELD OF INVENTION THIS INVENTION relates to a synthetic blowfly attractant.
BACKGROUND ART
Blowflies of the genus Lucilia and in particular the Australian sheep blowfly Lucilia cuprina cause flystrike in sheep which causes heavy financial costs to sheep owners as well as terrible suffering to infected animals. Protective methods at the present time include surgical operations on unanaesthetised animals such as "Mules' operation" and "pizzle dropping" which are relatively expensive and disliked by graziers and are campaigned against by animal welfare groups. Other protective methods depend upon application of chemicals to sheep which is undesirable because of the effect of the chemicals on human health, environmental problems and problems caused by insecticide residues in meat and wool. Also chemicals often select for resistance in blowflies.
In their Final Reports (Australian Government Printer: Canberra November 1991) the Australian Government's "Ecologically Sustainable Development Group on Agriculture" in Section 14.6 recommends the development of approaches for minimising chemical use in agriculture. Thus there is a need to develop sustainable systems for sheep blowfly management and a need to avoid the use of toxic pesticides because of the reasons referred to above, as well as the effect of the pesticides on non-target organisms.
The incidence of sheep flystrike is directly linked to fly population density as described in Wardhaugh and Morton Aust. J. Agric. Res. 4_1_ 1155-1167 in 1990. Their study has shown that at densities of near or below a standardised catch rate of one gravid female blowfly per standard trap per hour, the rate
and extent of sheep infestation would not generally be perceived as a major problem by graziers. Thus, suppression of female numbers is a prime management objective. It has been demonstrated that fly population suppression can be achieved with baiting or trapping e.g. for L. cuprina as described by Mackerras et al . J. Coun. Sci. Ind. Res. Aust. 9 153-162 in 1936 and also by Anderson et al . Aust. Vet. J. 6 93-97 in 1990. Laveissiere et al . in 1990 in a publication entitled "Appropriate Technology in Vector Control" pp 47-74 CRC Press, Boca Raton also came to the same conclusion in relation to tsetse flies. Foster et al . in an International Symposium on Management of Insect Pests in 1992 under the aegis of the International Atomic Energy Agency, FAO of the UN, Vienna, in a reference entitled "Advances in Sheep Blowfly Genetic Control in Australia" have calculated that an efficient trapping system for Lucilia blowflies would reduce by 57 to 70% the time required for eradication of Lucilia with genetic control.
The attractants used in the existing systems as described in the abovementioned Mackerras and Anderson references and also by Dadour and Cook in J. Aust. ent. Soc. 3J_ 205-208 in 1992 have the limitations of being based on organic materials e.g. aqueous liver suspensions and various bacteria. They have intrinsic problems, such as the attractiveness of a food type product to vertebrates and the health risk presented by bacterial cultures. These attractants require frequent servicing to overcome problems such as drying out and skin formation. An additional major drawback is their attractiveness for non- ucilia species of blowflies and for other insects, including beneficial insects, which is considered environmentally undesirable in the abovementioned Dadour and Cook reference.
Investigations on the improvement of organic attractants or the use of pure chemical attractants have been undertaken for Lucilia in, for example: (i) Freeney Pamph. Coun. Scient. Ind. Res. Aust. 2i 1-25 in 1937, (ii) Cragg and Ramage Parasitology J36_ 168- 175 in 1945, (iii) Eisemann in a PhD thesis on L. cuprina at The University of Queensland , (iv) Mulla in US Patent 3996349, (v) Mulla et al. in J. Chem. Ecol. TO 644-648 in 1977, (vi) Pickens et al . J. Med. Entomol. 1_0 84-88 in 1973 and (vii) Warner US Patent 5008107.
Freeney in reference (i) investigated the use of fatty acids, amines, sulfides and sodium sulfide for Lucilia species. Cragg and Ramage in reference (ii) tested the use of sheep wool as an attractant to which indole, ammonium carbonate and hydrogen sulfide had been added. Pickens in reference (vi) formulated a dry fly bait containing sucrose, sodium bicarbonate, yeast, dried blood, amyl acetate and sodium lactate which became active upon addition of water. The Mulla references (iv) and (v) developed a synthetic attractant for synanthropic flies consisting of triethylamine, an ammonium salt, linoleic acid and indole. This formulation also includes a 2-7 C hydrocarbon carboxylic acid for pH adjustment. Warner in reference (vii) utilised Mulla's formulation plus cis-9-tricosene. Eisemann in reference (iii) evaluated the response of L. cuprina towards a wide range of pure chemicals such as carboxylic acids, alcohols, thiols, amines, sulfides, phenols, hydrogen sulfide and indoles and various combinations thereof. Some of Eisemann' s binary and tertiary mixtures showed good attractancy for L. cuprina, but did not perform better than a standard liver/sodium sulfide bait. However, none of Eisemann' s mixtures used a combination of components (i), (ii), (iii) and (iv) in relation to the present invention as hereinafter
described.
In regard to the importance of development of traps for L. cuprina , it must be borne in mind the cost of prevention and production losses from blowfly strike in Australia in regard to sheep average $150 million per annum as shown in Beck Q. Rev. Rural Econ. 2 336-343 in 1985. Blowfly strike is also a problem in New Zealand as shown in Dymock et al . in NZ Journal of Agric. Res. 3_4 311-316 in 1991 and also in Great Britain as reported in Wall et al Bull. Entomol. Res.
82 125-131 in 1992 and also in South Africa as shown in Zumpt (1965) in a publication entitled "Myiasis in
Man and Animals in the Old World" Butterworth, London.
The Australian sheep blowfly L. cuprina initiates greater than 85% of sheep blowfly strike in Australia as shown in Anderson et al . Aust. J. Zool. 3J5 241-249 in 1988. Lucilia blowflies also cause problems around Australian suburbs as shown by Logan in a 1991 publication entitled "The Association of Blowflies with Wheelie-Bins in Darwin" published by the NT Department of Health and Community Services in Darwin. Lucilia blowflies also cause problems in American suburbs as discussed in Rice Ento. Soc. Qld. News Bull. 14 29-36 in 1986. Lucilia blowflies have also been implicated in strikes on hospital patients as discussed in Lukin Med. J. Aust. 150, 237-240 in 1989.
Fly attractant compositions are also described in US Patents 4,947,578; 4,959,209; 4,801,448; 4,911,906 and 4,638,592 as well as Japanese Specifications JP 48058126 and 76005448.
Specification EP 582915 describes an attractant for insects and especially Musca domestica which contains butyric or butanoic acid in synergistic combination with cis-9-tricosene which is a sex pheromone.
SUMMARY OF THE INVENTION It is therefore an object of the invention to provide a blowfly attractant lure formulation which is efficient in attracting blowflies which include blowflies of the genus Lucilia and which alleviates problems of prior art attractant lures as discussed above.
The attractant lure formulation of the invention includes: (i) one or more straight chain and/or branched chain C2-C8 carboxylic acids optionally containing hydroxyl, amino and/or thiol substituents; (ii) one or more unsubstituted or substituted straight or branched chain thiols including cycloaliphatic thiols having an upper limit of 12 carbons where the substituent(s) may be hydroxyl, amino including substituted amino such as NHR (where R is aliphatic), or additional thiol groups; (iii) one or more indoles or substituted indoles wherein there is a CM alkyl substituent at the 3 position or their analogues having the N replaced by S or the carbons at the 2 and/or 3 position replaced by N or S respectively; and (iv) hydrogen sulfide produced for example from an aqueous sulfide salt.
Each of the abovementioned components may be provided in a fly trap as a mixture with the exception of the sulfide salt solution which must remain separate. Alternatively the components may be provided as separate components in the trap.
Alternatively component (i) may be combined with component (ii) or component (iii) or in another situation components (ii) and (iii) may be combined.
However, an insect trap is only one option for effecting capture or otherwise processing attracted flies in association with the attractant lure formulation of the invention. Other options include toxicants, chemosterilants, growth regulators, pathogens, or electrified targets. These options may
be used either inside or outside an insect trap depending on contact requirements such as contact time or ingestion for these options to be effective.
An example of component (i) is butanoic acid; an example of component (ii) is 2-mercaptoethanol; an example of component (iii) is indole; and an example of component (iv) is sodium sulfide.
Other chemicals may be included in the four component lure formulation of the invention and these include straight or branched chain C2-C8 alcohols; dialkyl sulfides and/or dialkyl disulfides; phenols and/or substituted phenols; straight chain and/or branched chain C4-C10 aldehydes and straight chain or branched chain C3-C8 ketones. Preferred ranges of proportions of each of components (i) to (iii) are as follows in relation to forming a mixture -
(a) 10% to 80% of component (i);
(b) 10% to 80% of component (ii); (c) 2.5 to 20% of component (iii); whereby in each case the components (i), (ii) and (iii) add up to 100% or 100g of mixture on a weight/weight basis wherein each of the components are expressed in grams. The preferred proportions of the abovementioned components (i), (ii) and (iii) is as follows:
(a) 40-60% of component (i) and more preferably 49%,
(b) 30-50% of component (ii) more preferably 42%, and
(c) 5-15% of component (iii) and more preferably 9%. It will be appreciated that the above concentrations of components (i), (ii) and (iii) are calculated on a dry weight basis without taking into consideration the amount of solvent that may be used. The solvent used may be any appropriate biologically or chemically inert solvent. Examples of suitable solvents are water, glycerol, ethylene glycol, propylene glycol or other suitable solvent.
In relation to component (iv) any sulfide salt that emits H2S preferably in the presence of water would be effective. These include calcium sulfide, magnesium sulfide, potassium sulfide, and sodium sulfide.
An effective release of hydrogen sulfide is achieved by means of a 5-50% by weight solution of sodium sulfide in water.
When using technical grade sodium sulfide which has 68% sodium sulfide, suitably 10-25% by weight of technical grade sodium sulfide in water is utilized. However when using analytical grade sodium sulfide or hydrated sodium sulfide suitably 25-50% by weight of hydrated sodium sulfide in water is utilized. The above solutions release hydrogen sulfide for example from a wick in a bottle. Hydrogen sulfide released from such a wick into an air stream above the bottle has been measured at approximately 1 ppm.
However more broadly a range of sulfide solutions in water could be from a minimum of 0.5% up to very concentrated solutions i.e. saturated or supersaturated solutions.
However, it will be appreciated that there are other methods for releasing attractant mixtures or components into the atmosphere than the use of a wick. These include open bottles, solid supports soaked with attractant, microencapsulation or sachet dispensers.
The release rate of attractant has a great effect on the number of flies caught whereby the greater the release rate will mean a higher catch of flies. In a commercial trap the release rate of mixture (i), (ii) and (iii) is approximately 500 mg per day. But this can be varied over wide limits - i.e. a maximum release rate may be several grams per day and a minimum release rate may be of the order of a few mg per day.
BRIEF DESCRIPTION OF DRAWINGS FIG 1 illustrates the synergistic effect between butanoic acid (component (i)) and the other attractant components (ii), (iii) and (iv) on the attractiveness for L. cuprina . The number of L . cuprina caught by the attractant including butanoic acid is 2.7 x higher than the sum of the flies caught by butanoic acid alone and caught by the attractant mixture without the butanoic acid. FIG 2 refers to a graph of an experiment described hereinafter concerning capture of L. cuprina in traps incorporating the attractant lure formulation of the invention.
DETAILED DESCRIPTION In order to enable the invention to be fully understood, a number of preferred embodiments will now be described which are contained in the following Examples.
EXAMPLE 1 500 ml of an aqueous synthetic attractant mixture containing 5% 2-mercaptoethanol, 5% butanoic acid, 5% pentanoic acid, 0.5% indole [i.e. 64% of component (i) 33% of component (ii) and 3% of component (iii)] and a separate 20% sodium sulfide solution in water was tested for five days in an insectary against a standard blowfly attractant (500 gm of bovine liver and 500 ml of a 1.5% solution of sodium sulfide). The synthetic attractant attracted significantly more flies (3.3 x more with P<0.05). EXAMPLE 2
A synthetic attractant mixture containing 0.5% 2- mercaptoethanol, 0.1% butanoic acid, 0.05% indole [i.e. 15% component (i) 78% of component (ii) and 7% of component (iii)] and 0.2% separate sodium sulfide solution was tested over 7 days in an insectary and attracted 24 x as many flies as 500 ml of a commercial "Efekto" bait.
EXAMPLE 3
In sheep paddocks at Cunnamulla using a 6 x 6 Latin square design over 6 days in October 1991 a synthetic attractant containing a mixture of 8 gm 2- ercaptoethanol, 2 gm butanoic acid, 1 gm indole [i.e. 18% of component (i), 72% of component (ii) and 10% of component (iii)] and a separate 10 ml saturated sodium sulfide solution caught 2.7 x as many wild Lucilia cuprina as a standard blowfly attractant (10 gm of bovine liver and 10 ml of a 1.5% sodium sulfide solution). The synthetic attractant was also more highly selective for Lucilia because it caught over 8 x less Chrysoma and much less Calliphora than Lucilia, compared to the liver/sodium sulfide standard. EXAMPLE 4
The same attractants as in 3. were compared in suburban Brisbane, Australia, in March 1992 by a duplicated 4 x 4 Latin square over 4 days. The synthetic attractant caught 8.4 x as many Lucilia cuprina as the standard liver attractant. It also caught 6.8 x less Chrysomya and 8.6 x less Calliphora than Lucilia, when compared with the liver/sodium sulfide standard.
EXAMPLE 5 The value of including all four different types of chemicals in the synthetic attractant was demonstrated in an automated insectary by pairwise comparisons of the lure mixtures described in Table 1 herein. EXAMPLE 6
Field tests at Charleville, Australia, in October
1990 using 4 x 4 Latin square replicated comparisons on variations of the base attractant (pentanoic acid, 2-mercaptoethanol, indole; sodium sulfide solution) showed that without sodium sulfide the catch was
reduced more than 6 times and that without pentanoic acid the catch was reduced more than 15 times.
EXAMPLE 7
In addition to the four major attractant chemicals exemplified above other chemicals have proved able to significantly (P<0.05) increase the catch of Lucilia cuprina in an insectary. A 31% increase over the base attractant catch was obtained by the inclusion of butan-2-ol and 2-methylpropane-1- ol. An 11% increase was obtained by inclusion of cis-
3-octen-1-ol. A 26% increase was obtained by inclusion of hexanal, 2-octanone and benzaldehyde.
EXAMPLE 8
Urban tests in Brisbane, Australia in duplicated 4 x 4 Latin square comparisons showed that addition to the basic four component synthetic attractant (2- mercaptoethanol , indole, butanoic acid, sodium sulfide) of phenol, 4-methylphenol, butan-2-ol, 2- methylpropane-1-ol, dimethyl disulfide increased the catch of Lucilia cuprina 2.2 x. Addition to the basic attractant of butan-2-ol, 2-methylpropane-1-ol, pentanoic acid, acetic acid and acetone increased the catch of Lucilia cuprina 1.2 x.
EXAMPLE 9 Small scale field trials were carried out in western Queensland to test the hypothesis that placing traps with a synthetic attractant (2-mercaptoethanol, indole, butanoic acid, sodium sulfide) in a sheep
paddock would lower the Australian sheep blowfly population in that paddock. The trial was run with five replicates (Cunnamulla, Charleville, Roma, Longreach [2 x] ) each consisting of two paired sites, one as control (no traps) and the other containing traps. In each site five monitoring traps (opened for 24 hours every seven days) were used to measure the fly population. The mean numbers (from all five replicates) of sheep blowflies caught in the monitoring traps in the trapped and the control areas during the 15 week trial are shown in figure 2. The fly population was substantially lower in the trapped area, indicating population suppression by the presence of the synthetic attractant in a trap. EXAMPLE 10
The use of various formulations as described in Table 2 where also tested in an automated insectary with the results as shown. In an automated insectary the number of flies on a target were monitored when presented with vapours containing the attractant lure formulation of the invention. This is equivalent to a response and a statistical analysis is carried out on a comparison between different attractant lure formulations. The automated insectary was used above in Examples 1-2, 5 and 7 and is a part of or associated with apparatus entitled Behavioural Observation Facility for Flies (BOFF) and is well known to the
skilled addressee.
EXAMPLE 11 Further trials were carried out using various formulations in accordance with the invention on a 4x4 Latin Square and the results of these trials are reported in Table 3.
TABLE 1
Composition Mean response # (number of flies)
Lure 1 Lure 2 Lure 1 Lure 2
2-mercaptoethanol 2-mercaptoethanol 38.9 100.2 indole indole sodium sulfide
2-mercaptoethanol 2-mercaptoethanol 11.1 33.0 indole indole pentanoic acid pentanoic acid sodium sulfide
2-mercaptoethanol 2-mercaptoethanol 48.3 57.5 indole indole sodium sulfide sodium sulfide pentanoic acid
2-mercaptoethanol 2-mercaptoethanol 47.5 96.3 butanoic acid butanoic acid sodium sulfide sodium sulfide indole
# Differences between treatments were significant (P<0.05) in all cases.
TABLE 2
Automated insectary
Attractant Response* Ratio of responses
2-mercaptoethanol (0.5%), indole (0.05%), butanoic acid 9123A (0.1%); sodium sulfide (0,2%)
1.04
2-mercaptoethanol (0.5%), indole (0.05%), butanoic acid 8789* (0.1%), pentanoic acid (0.1%); sodium sulfide (0.2%)
2-mercaptoethanol (0.5%), indole (0.05%), butanoic acid 38123A (0.1%); sodium sulfide (0.2%)
1.49
2-mercaptoethanol (0.5%), indole (0.05%), 3-methylbutanoic 25657B acid (0.1%); sodium sulfide (0.2%)
2-mercaptoethanol (0.5%), indole (0.05%), butanoic acid 9498A (0.1%); sodium sulfide (0.2%)
1.39
2-mercaptoethanol (0.5%), indole (0.05%), hexanoic acid 6852B (0.1%); sodium sulfide (0.2%)
2-mercaptoethanol (0.5%), indole (0.05%), butanoic acid 6687A (0.1%); sodium sulfide (02%)
1.58
2-mercaptoethanol (0.5%), indole (0.05%), thiolactic acid 4237B (0.3%); sodium sulfide (0.2%)
Within one experiment, responses with the same superscript do not differ significandy (P>0.05)
TABLE 3
4 x 4 Latin square, Brisbane, February 1992
Attractant Mean number of
L. cuprina per trap per day*
2-mercaptoethanol (41%), indole (9%), butanoic acid (50%); 5 - sodium sulfide
2-meιcaptoethanol (41%), indole (9%), butanoic acid (10%), 25A propanoic acid (10%), pentanoic acid (10%), hexanoic acid (10%), octanoic acid (10%); sodium sulfide
Responses with the same superscript do not differ significandy (P 0.05)
Legends Figure 1
Synergism with butanoic acid - Brisbane field trial December 1992, 6x6 Latin square Attractants:
1. Butanoic acid (100%).
2. 2-Mercaptoethanol (89%), indole (11%), sodium sulfide solution.
3. 2-Mercaptoethanol (71%), indole (9%), butanoic acid (20%), sodium sulfide solution.
Figure 2
Mean Lucilia cuprina trap catches per trap per day in monitoring traps located in control and treated (trapped) areas during field trials in Western Queensland September-December 1992.