WO1993019366A1 - Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding - Google Patents
Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding Download PDFInfo
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- WO1993019366A1 WO1993019366A1 PCT/US1993/002470 US9302470W WO9319366A1 WO 1993019366 A1 WO1993019366 A1 WO 1993019366A1 US 9302470 W US9302470 W US 9302470W WO 9319366 A1 WO9319366 A1 WO 9319366A1
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- IBZUISWMZGLPKG-UHFFFAOYSA-N phosphoric acid azide Chemical compound [N-]=[N+]=[N-].OP(O)(O)=O IBZUISWMZGLPKG-UHFFFAOYSA-N 0.000 description 1
- XQZYPMVTSDWCCE-UHFFFAOYSA-N phthalonitrile Chemical compound N#CC1=CC=CC=C1C#N XQZYPMVTSDWCCE-UHFFFAOYSA-N 0.000 description 1
- 229920006391 phthalonitrile polymer Polymers 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- LPNYRYFBWFDTMA-UHFFFAOYSA-N potassium tert-butoxide Chemical compound [K+].CC(C)(C)[O-] LPNYRYFBWFDTMA-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 239000011546 protein dye Substances 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- AOJFQRQNPXYVLM-UHFFFAOYSA-N pyridin-1-ium;chloride Chemical compound [Cl-].C1=CC=[NH+]C=C1 AOJFQRQNPXYVLM-UHFFFAOYSA-N 0.000 description 1
- ZOKPDCKJLXVQPL-UHFFFAOYSA-N pyridine;hydrochloride Chemical compound Cl.C1=CC=NC=C1.C1=CC=NC=C1 ZOKPDCKJLXVQPL-UHFFFAOYSA-N 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- IOVGROKTTNBUGK-SJCJKPOMSA-N ritodrine Chemical class N([C@@H](C)[C@H](O)C=1C=CC(O)=CC=1)CCC1=CC=C(O)C=C1 IOVGROKTTNBUGK-SJCJKPOMSA-N 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000003375 selectivity assay Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000005049 silicon tetrachloride Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000008279 sol Substances 0.000 description 1
- 238000002764 solid phase assay Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 108020003113 steroid hormone receptors Proteins 0.000 description 1
- 102000005969 steroid hormone receptors Human genes 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003239 susceptibility assay Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 229910052718 tin Inorganic materials 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000000954 titration curve Methods 0.000 description 1
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- 238000011282 treatment Methods 0.000 description 1
- 229940086542 triethylamine Drugs 0.000 description 1
- 239000000439 tumor marker Substances 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
- A61K49/0036—Porphyrins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
- A61K49/0052—Small organic molecules
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic System
- C07F7/02—Silicon compounds
- C07F7/08—Compounds having one or more C—Si linkages
- C07F7/0834—Compounds having one or more O-Si linkage
- C07F7/0838—Compounds with one or more Si-O-Si sequences
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
- G01N2333/19—Rubella virus
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP93908390A EP0632893A4 (en) | 1992-03-23 | 1993-03-23 | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding. |
CA002132708A CA2132708C (en) | 1992-03-23 | 1993-03-23 | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding |
US08/035,633 US5846703A (en) | 1990-05-15 | 1993-03-23 | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US85617692A | 1992-03-23 | 1992-03-23 | |
US07/856,176 | 1992-03-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1993019366A1 true WO1993019366A1 (en) | 1993-09-30 |
Family
ID=25323005
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1993/002470 WO1993019366A1 (en) | 1990-05-15 | 1993-03-23 | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0632893A4 (en) |
CA (1) | CA2132708C (en) |
WO (1) | WO1993019366A1 (en) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996029367A1 (en) * | 1995-03-23 | 1996-09-26 | Biosite Diagnostics Incorporated | Hybrid phthalocyanine derivatives and their uses |
WO1996041144A2 (en) * | 1995-06-06 | 1996-12-19 | Hyperion, Inc. | Polyoxyhydrocarbyl marker products and methods for fluorescence assays |
WO1998030908A1 (en) * | 1997-01-08 | 1998-07-16 | Dade Behring Inc. | Luminescent specific binding assay |
US5824799A (en) * | 1993-09-24 | 1998-10-20 | Biosite Diagnostics Incorporated | Hybrid phthalocyanine derivatives and their uses |
US5919922A (en) * | 1990-05-15 | 1999-07-06 | Hyperion, Inc. | Fluorescent dyes free of aggregation and serum binding |
US6060598A (en) * | 1990-05-15 | 2000-05-09 | Hyperion, Inc. | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding |
WO2000031187A1 (en) * | 1998-11-25 | 2000-06-02 | Hyperion, Inc. | Water soluble fluorescent dyes free of aggregation and serum binding and related products and methods |
US6238931B1 (en) | 1993-09-24 | 2001-05-29 | Biosite Diagnostics, Inc. | Fluorescence energy transfer in particles |
US7083984B2 (en) | 1993-09-24 | 2006-08-01 | Biosite, Inc. | Hybrid phthalocyanine derivatives and their uses |
EP1780213A1 (en) * | 2005-10-27 | 2007-05-02 | 3M Innovative Properties Company | Silicon-urea-azolides, their preparation and use in the preparation of silicones with isocyanate terminal groups |
US7322927B2 (en) | 1993-09-24 | 2008-01-29 | Biosite, Inc. | Hybrid phthalocyanine derivatives and their uses |
EP2343974A4 (en) * | 2008-08-15 | 2015-07-15 | Univ Georgetown | Na channels, disease, and related assays and compositions |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988004777A1 (en) * | 1986-12-15 | 1988-06-30 | Ultra Diagnostics Corporation | Monomeric phthalocyanine reagents |
US4822877A (en) * | 1986-02-05 | 1989-04-18 | Suntory Limited | Modified haem |
WO1991018006A1 (en) * | 1990-05-15 | 1991-11-28 | Diatron Corporation | Fluorescent porphyrin, and fluorescent phthalocyanine - polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes |
WO1991018007A1 (en) * | 1990-05-15 | 1991-11-28 | Diatron Corporation | Phthalocyanatopolyethylene glycol, and phthalocyanato saccharides as fluorescent digoxin reagents |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69214328T2 (en) * | 1991-03-05 | 1997-02-20 | Hitachi Chemical Co Ltd | Water soluble tetrazaporphin and fluorescent label |
-
1993
- 1993-03-23 EP EP93908390A patent/EP0632893A4/en not_active Ceased
- 1993-03-23 CA CA002132708A patent/CA2132708C/en not_active Expired - Fee Related
- 1993-03-23 WO PCT/US1993/002470 patent/WO1993019366A1/en not_active Application Discontinuation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4822877A (en) * | 1986-02-05 | 1989-04-18 | Suntory Limited | Modified haem |
WO1988004777A1 (en) * | 1986-12-15 | 1988-06-30 | Ultra Diagnostics Corporation | Monomeric phthalocyanine reagents |
WO1991018006A1 (en) * | 1990-05-15 | 1991-11-28 | Diatron Corporation | Fluorescent porphyrin, and fluorescent phthalocyanine - polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes |
WO1991018007A1 (en) * | 1990-05-15 | 1991-11-28 | Diatron Corporation | Phthalocyanatopolyethylene glycol, and phthalocyanato saccharides as fluorescent digoxin reagents |
Non-Patent Citations (3)
Title |
---|
Clinical Chemistry, Vol. 31, No. 3, issued 1985, HEMMILA, "Fluoroimmunoassays and Immunofluorometric Assays", pages 359-370, see page 362, Table 1, and pages 363 and 366. * |
Journal of Clinical Immunoassay, Vol. 13, No. 2, KOLLS, "Quality Control in Practice: Non-specific Binding and High Dose Hook", pages 87-88, see entire document. * |
See also references of EP0632893A4 * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5919922A (en) * | 1990-05-15 | 1999-07-06 | Hyperion, Inc. | Fluorescent dyes free of aggregation and serum binding |
US6060598A (en) * | 1990-05-15 | 2000-05-09 | Hyperion, Inc. | Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding |
US7322927B2 (en) | 1993-09-24 | 2008-01-29 | Biosite, Inc. | Hybrid phthalocyanine derivatives and their uses |
US5824799A (en) * | 1993-09-24 | 1998-10-20 | Biosite Diagnostics Incorporated | Hybrid phthalocyanine derivatives and their uses |
US6238931B1 (en) | 1993-09-24 | 2001-05-29 | Biosite Diagnostics, Inc. | Fluorescence energy transfer in particles |
US7083984B2 (en) | 1993-09-24 | 2006-08-01 | Biosite, Inc. | Hybrid phthalocyanine derivatives and their uses |
WO1996029367A1 (en) * | 1995-03-23 | 1996-09-26 | Biosite Diagnostics Incorporated | Hybrid phthalocyanine derivatives and their uses |
WO1996041144A2 (en) * | 1995-06-06 | 1996-12-19 | Hyperion, Inc. | Polyoxyhydrocarbyl marker products and methods for fluorescence assays |
WO1996041144A3 (en) * | 1995-06-06 | 1997-02-06 | Hyperion Inc | Polyoxyhydrocarbyl marker products and methods for fluorescence assays |
WO1998030908A1 (en) * | 1997-01-08 | 1998-07-16 | Dade Behring Inc. | Luminescent specific binding assay |
US5876935A (en) * | 1997-01-08 | 1999-03-02 | Dade Behring Inc. | Luminescent specific binding assay |
WO2000031187A1 (en) * | 1998-11-25 | 2000-06-02 | Hyperion, Inc. | Water soluble fluorescent dyes free of aggregation and serum binding and related products and methods |
WO2007050950A2 (en) * | 2005-10-27 | 2007-05-03 | 3M Innovative Properties Company | Silicon-urea-azolides, their preparation and use in the preparation of silicones with isocyanate terminal groups |
WO2007050950A3 (en) * | 2005-10-27 | 2007-06-14 | 3M Innovative Properties Co | Silicon-urea-azolides, their preparation and use in the preparation of silicones with isocyanate terminal groups |
EP1780213A1 (en) * | 2005-10-27 | 2007-05-02 | 3M Innovative Properties Company | Silicon-urea-azolides, their preparation and use in the preparation of silicones with isocyanate terminal groups |
US8124714B2 (en) | 2005-10-27 | 2012-02-28 | 3M Innovative Properties Company | Silicon-urea-azolides, their preparation and use in the preparation of silicones with isocyanate terminal groups |
EP2343974A4 (en) * | 2008-08-15 | 2015-07-15 | Univ Georgetown | Na channels, disease, and related assays and compositions |
Also Published As
Publication number | Publication date |
---|---|
CA2132708A1 (en) | 1993-09-30 |
EP0632893A1 (en) | 1995-01-11 |
EP0632893A4 (en) | 1997-01-02 |
CA2132708C (en) | 2005-05-10 |
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