WO1991014003A3 - Characterization and analysis of polymorphic vntr loci - Google Patents

Characterization and analysis of polymorphic vntr loci Download PDF

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Publication number
WO1991014003A3
WO1991014003A3 PCT/US1991/001628 US9101628W WO9114003A3 WO 1991014003 A3 WO1991014003 A3 WO 1991014003A3 US 9101628 W US9101628 W US 9101628W WO 9114003 A3 WO9114003 A3 WO 9114003A3
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WO
WIPO (PCT)
Prior art keywords
characterization
locus
vntr
analysis
polymorphisms
Prior art date
Application number
PCT/US1991/001628
Other languages
French (fr)
Other versions
WO1991014003A2 (en
Inventor
Glenn T Horn
Brenda L Richards
Original Assignee
Ig Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ig Lab Inc filed Critical Ig Lab Inc
Publication of WO1991014003A2 publication Critical patent/WO1991014003A2/en
Publication of WO1991014003A3 publication Critical patent/WO1991014003A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

This invention pertains to characterization of the YNZ32 VNTR locus and to methods for detecting polymorphisms at a highly polymorphic VNTR locus. Polymorphisms are detected by VNTR locus amplification and enzymatic digestion of the amplification products. Variations observed in restriction maps of the fragments are then used to assess the polymorphisms. Primers for VNTR amplification of the YNZ32 locus are described.
PCT/US1991/001628 1990-03-09 1991-03-11 Characterization and analysis of polymorphic vntr loci WO1991014003A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49128690A 1990-03-09 1990-03-09
US491,286 1990-03-09

Publications (2)

Publication Number Publication Date
WO1991014003A2 WO1991014003A2 (en) 1991-09-19
WO1991014003A3 true WO1991014003A3 (en) 1991-10-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/001628 WO1991014003A2 (en) 1990-03-09 1991-03-11 Characterization and analysis of polymorphic vntr loci

Country Status (1)

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WO (1) WO1991014003A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
AU672760B2 (en) * 1991-09-24 1996-10-17 Keygene N.V. Selective restriction fragment amplification: a general method for DNA fingerprinting
US7026115B1 (en) 1991-09-24 2006-04-11 Keygene N.V. Selective restriction fragment amplification: fingerprinting
IL103935A0 (en) * 1991-12-04 1993-05-13 Du Pont Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6808633B1 (en) 1999-06-23 2004-10-26 Hitachi, Ltd. Centrifugal separator and sample preparation device using the separator
US20030031675A1 (en) 2000-06-06 2003-02-13 Mikesell Glen E. B7-related nucleic acids and polypeptides useful for immunomodulation
EP1950305A1 (en) 2001-05-09 2008-07-30 Monsanto Technology, LLC Tyr a genes and uses thereof
US7329490B2 (en) 2003-09-15 2008-02-12 Bristol-Myers Squibb Company Methods for diagnosing schizophrenia by detecting a polymorphism in the KalphaM1 gene
CA2695264C (en) 2007-08-01 2015-01-27 Dana-Farber Cancer Institute Enrichment of a target sequence
CN102859003B (en) 2010-03-08 2014-07-30 达纳-法伯癌症研究院有限公司 Full COLD-PCR enrichment with reference blocking sequence
US20130316358A1 (en) 2011-01-31 2013-11-28 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
EP3333269B1 (en) 2011-03-31 2021-05-05 Dana-Farber Cancer Institute, Inc. Methods to enable multiplex cold-pcr
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2015013166A1 (en) 2013-07-24 2015-01-29 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor dna alleles by limiting denaturation time in pcr or simply enable enrichment of minor dna alleles by limiting denaturation time in pcr
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying dna targets in a single reaction mixture

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007658A1 (en) * 1988-02-18 1989-08-24 University Of Utah Genetic identification employing dna probes of variable number tandem repeat loci
EP0370719A2 (en) * 1988-11-25 1990-05-30 Imperial Chemical Industries Plc Extended nucleotide sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007658A1 (en) * 1988-02-18 1989-08-24 University Of Utah Genetic identification employing dna probes of variable number tandem repeat loci
EP0370719A2 (en) * 1988-11-25 1990-05-30 Imperial Chemical Industries Plc Extended nucleotide sequences

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biological Abstracts, volume 91, no. 8, April 1991, B. Richards et al.: "Characterization and rapid analysis of the highly poly-morphic VNTR locus D4S125 (YNZ32), closely linked to the huntington disease gene", see page 461 *
Nucleic Acids Research, volume 17, no. 5, March 1989, IRL Press, G.T. Horn et al.: "Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction", page 2140 *
Science, volume 235, March 1987, Yusuke Nakamura et al.: "Variable number of tandem repeat (VNTR) markers for human gene mapping", pages 1616-1622 *

Also Published As

Publication number Publication date
WO1991014003A2 (en) 1991-09-19

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