WO1989005830A1

WO1989005830A1 - Biodegradable polyurethanes, products based thereon, and polyester polyol prepolymers

Info

Publication number: WO1989005830A1
Application number: PCT/NL1988/000060
Authority: WO
Inventors: Albert Johan Pennings
Original assignee: Stichting Biomat
Priority date: 1987-12-23
Filing date: 1988-12-22
Publication date: 1989-06-29
Also published as: NL8703115A; EP0417095A1

Abstract

This invention relates to biodegradable polyurethanes on the basis of a polyol prepolymer and an L-lysine derivative having at least 2 isocyanate groups. The polyol prepolymer is preferably a polyester polyol prepolymer obtained by ring-opening polymerization of L-lactide, glycolide and/or lactone with a cyclic polyol. These new polyurethanes can be used in the production of different types of biomedical products, such as artificial skin, wound dressing, artificial veins, nerve grafts etc.

Description

Title: Biodegradable polyurethanes, products based thereon, and polyester polyol prepolymers

This invention relates to a biodegradable polyurethane on the basis of a polyol prepolymer and a polyisocyanate.

The invention further relates to products, especially biomedical products, such as artificial skin, wound dressings, artificial veins, vein grafts, nerve grafts, etc., comprising such a polyurethane.

Furthermore, the invention relates to a polyester polyol prepolymer adapted for use in the preparation of the polyurethane. Polyurethanes are considered to be excellent biomedical materials having good mechanical and physical properties and showing a satisfactory blood compatibility. For these reasons linear (thermoplastic) elastomeric polyurethanes are used, e.g., in biodegradable polyurethanes/poly(L-lactide) blends for the production of biomedical products, such as grafts for blood vessels, meniscus prostheses, artificial skin products and nerve grafts. See, e.g., Gogolewski and Pennings, Makro ol. Chem. , Rapid Commun. 3 (1982) 839 and 4 (1983) 675. The prior art polyurethane/poly(L-lactide) compositions, however, show ^n_ vivo, after initial fragmentation, a very low rate of degradation. Besides, dynamic load will give rise to creep conditions leading to aneurysms in grafts for blood vessels. An even more important disadvantage of the polyurethane elastomers known for biomedical uses with biodegradation, such as Biomer, Estane, etc., is that toxic, mutagenic and carcinogenic substances, such as 4,4'-methylenedianiline when the polyurethane has been prepared using ,4'-methylene diphenyl diisocyanate, may be released upon degradation. It is well-known that this disadvantage can be mitigated by using non-aromatic polyisocyanates in the preparation of the polyurethanes. Szycher et al., J. Elastomers and Plastics 15 (1983) 81-95, for instance, propose to use cycloaliphatic diisocyanates, such as 4,4'-methylene-bis-cyclohexyl diisocyanate. Reaction ; of this diisocyanate with a polytetramethylene ether glycol (having a molecular weight of about 1000) and . 1,4-butanediol gives biomedical grade cycloaliphatic polyurethane elastomers sold under the trade name Tecoflex. It has also been proposed to use non-cyclic aliphatic polyisocyanates, such as 1,6-hexane diisocyanate, in the preparation of polyurethane. The corresponding diamin.es that may be released upon degradation of the polyurethanes, however, are more or less still toxic.

With regard to the polyols to be used in the preparation of polyurethanes, it is known to use polyester polyol prepolymers for this purpose. Thus, for instance, Schindler et al., J. Polym. Sci. 20 (1982) 319 describe the forming of stellate polycaprolactone polymers by an alcohol-initiated ring-opening polymerization of ε-caprolactone, the alcohol used being a cyclic sugar, such as sorbitol, xylitol or ribitol. Pitt et al., J. Polym. Sci. 25 (1987) 955 describe the preparation of biodegradable polyurethanes from prepolymers having

3 terminal hydroxyl groups obtained by a glycerol-initiated ring-opening copoly erization of a 1 : 1 mixture of δ-valerolactone and ε-caprolactone. These prepolymers are crosslinked with 1,6-hexane diisocyanate. Furthermore, Schindler et al., in "Cont. Topics in Polym. Sci." (Eds. Pearce and Schaefgen) Plenum Press N.Y., USA, vol. 2 (1977) and Kricheldorf et al., Macromolecules 17 (1984) 2173 already describe biodegradable copolyesters of L-lactide or glycolide and ε-caprolactone. However, biodegradable polyurethane elastomers having a combination of properties suitable for different biomedical uses and especially giving no toxic degradation products upon degradation have not been described so far. This invention provides a biodegradable polyurethane on the basis of a polyol prepolymer and a polyisocyanate which satisfies this need and is characterized in that the polyisocyanate is an L-lysine derivative having at least 2 isocyanate groups. More in particular, this invention relates to such a biodegradable polyurethane in which the polyol prepolymer is a polyester polyol prepolymer. The L-lysine derivative to be used according to the invention in the preparation of the polyurethane is preferably a compound having the structural formula ■

in which R is an alkyl-, aryl-, alkaryl- or aralkyl group, which may be substituted by one or more isocyanate groups and/or groups inert in the polyurethane-forming reaction, such as alkoxy groups. It is preferred that

R is a lower alkyl group, most preferably the ethyl-group, and that this lower alkyl group is not substituted or substituted by an isocyanate group.

Such L-lysine polyisocyanates are known per se from French patent 1,351,368, which also describes their preparation and their usability in the preparation of polyurethane foams, adhesives and elastomers. This publicatio however, in no way suggests the conversion of such L-lysine polyisocyanates with polyol prepolymers, especially polyester polyol prepolymers, to biomedically applicable polyurethanes.

The polyurethane according to the invention may be based on a polyester diol prepolymer and an L-lysine derivative having at least 3 isocyanate groups, preferably L-lysine a inoethyl ester triisocyanate having the structural formula OCN-CH-COO ( CH₂ ) 2NCO

( CH )

I NCO

The polyester diol prepolymer is preferably a prepolymer obtained by a ring-opening polymerization reaction of

L-lactide, glycolide and/or one or more lactones, such as ε-caprolactone and 6-valerolactone, if desired initiated with a diol, such as glycol, 1,4-butanediol, 1,6-hexanediol, etc. Most preferably, a prepolymer is obtained by a ring-opening copolymerization, if desired initiated with a diol, of L-lactide and/or glycolide with one or more lactones, such as ε-caprolactone and δ-valerolactone.

The invention, however, also comprises linear or non-linear biodegradable polyurethanes based on an L-lysine derivative having 2 or more isocyanate groups, such as L-lysine ethyl ester diisocyanate and L-lysine aminoethyl ester triisocyanate; a diol prepolymer, e.g., a polyether diol, such as polytetramethylene glycol having a molecular weight of about 1000-2000, or a polyester diol, such as polycaprolactone diol having a molecular weight of about 1000-2000; and a chain extender, such as 1,4-butane diol.

The polyurethane according to the invention is, however, preferably based on an L-lysine diisocyanate, most preferably L-lysine ethyl ester diisocyanate having the structural formula

and a polyester polyol prepolymer having at least 3 hydroxyl groups. This polymer is preferably obtained by a ring-opening polymerization reaction of L-lactide^~, glycolide, and/or one or more lactones, such as ε-capro¬ lactone and δ-valerolactone, with a polyol containing at least 3 hydroxyl groups. In this case, too, a copoly- merization of L-lactide and/or glycolide with one or more lactones, such as ε-caprolactone and δ-valerolactone,. is preferred. The polyol to be used to initiate the polymerization reaction is preferably a cyclic polyol and in particular myo-inositol has proved to be eminently suited for this purpose, in relation to the contemplated uses of the polyurethane.

Most preferred is a polyurethane according to- the invention, in which the polyester polyol prepolymer is obtained by a ring-opening polymerization reaction of L-lactide or glycolide with ε-caprolactone and with myo-inositol, and the L-lysine polyisocyanate is the compound L-lysine ethyl ester diisocyanate. The monomeric products that may be released upon full degradation of such a polyurethane are myo-inositol (a vitamin occurring in human beings), L-lactic acid or glycolic acid, 6-hydroxy- hexanoic acid, L-lysine and ethanol. These monomeric compounds are all non-toxic, which is of great importance to the use of the polyurethane as a degradable biomedical material. The second advantage due to the use of L-lysine ethyl ester diisocyanate is that the carboxyl group formed in the hydrolysis of the ethyl ester has a catalytic effect on the further degradation of the polymer. The advantage of L-lactide (or glycolide)/ε-caprolactone copolyester prepolymers is that the polyurethanes based thereon combine good elastomeric properties with a high rate of biodegradation. Polyurethanes on the basis of L-lactide/myo-inositol or glycolide/myo-inositol prepolymers have glass transition temperatures (Tg) above room temperature while polyurethanes on the basis of poly( ε-caprolactone) prepolymers have a low rate of biodegradation, which is undesirable for many uses. The polyurethanes preferred according to the invention on the basis of copolyester prepolymers preferably containing approximately equimolar amounts of L-lactide (or glycolide) and ε-caprolactone combine low glass transition temperatures, e.g., within the range of 0-10°C, with a relatively high rate of biodegradation. The glass transition temperature can be adjusted to a desired value by controlling the chain lengths of the copolyester prepolymers; greater chain lengths lead to lower values of the glass transition temperature. In this connection the fact is to be considered that residues of non-reacted monomers and oligomers show a plasticizer effect, so that an extraction treatment of the polymer, e.g., with chloroform, as a result of the removal of such monomers and oligomers, will lead to a somewhat higher glass transition temperature.

These residues of monomers and oligomers also affect the gel content of the polyurethanes according to the invention. The highest gel contents are obtained when the prepolymer, prior to its reaction with the L-lysine polysocyanate, is liberated from such low molecular residues by precipitating the prepolymer by means of a non-solvent, such as ethanol, from a solution of the prepolymer in an organic solvent, such as chloroform. Higher gel contents can also be obtained by using a slight excess of the isocyanate groups over the hydroxyl groups. Besides forming urethane links, an additional crosslinking may occur by means of forming allophanate groups.

In connection with different biomedical uses, e.g., as a base for artificial skin products for covering wounds (burns), or as an internal layer of blood vessel and nerve grafts, the polyurethane according to the invention preferably consists of a porous network. Such a porous polyurethane network is obtainable by carrying out the.reaction between polyester polyol prepolymer and L-lysine polyisocyanate in the presence of a salt, such as sodium chloride, and removing this salt later, e.g., by treatment with water, whereby pores remain.

This invention also comprises products which completely or partly consist of a polyurethane according to the invention, in particular sheet-like biomedical products, such as artificial skin, wound dressings etc. and tubular biomedical products, such as artificial veins, vein grafts, nerve grafts etc. Furthermore, the polyurethanes according to the invention can also be used as a biodegradable carrier for medicaments etc., as a (component of) biodegradabl suture, etc.

The invention also extends to a polyester polyol prepolymer on the basis of (1) a cyclic polyhydroxy compound having at least 3 functional hydroxyl groups, (2) L-lactide and/or glycolide, and (3) one or more lactones, such as ε-caprolactone and δ-valerolactone, especially to such a prepolymer in which the polyhydroxy compound is myo-inositol and/or the lactone is ε-caprolactone. An example serves to illustrate the invention.

Example

(a) Preparation of the prepolymers

L-lactide (sold by C.C.A., Gorinchem, The Netherlands; after recrystallization from dry toluene) or glycolide (sold by Dupont), as well as ε-caprolactone (sold by Janssen Chemical, Belgium; after distillation) and myo- inositol (sold by Merck) were dissolved in dry dimethyl- forma ide at 140°C. As a catalyst 0.5 % by weight of tin(II)octoate (sold by Sigma Che . Corp. USA) were added, and the polymerization was carried out for 20 hours at 120-130°C under a nitrogen atmosphere. After removal of the solvent under reduced pressure a sticky, yellowish prepolymer remained. A part of this prepolymer was precipitated in ethanol (-70°C) from a solution in chloroform and dried at room temperature under reduced pressure.

(b) Preparation of L-lysine ethyl ester diisocyanate

L-lysine monohydrochloride (sold by Janssen Chemical, Belgium) was converted to L-lysine ethyl ester dihydrochloride by boiling in ethanol under conditions of reflux while passing HC1 gas through the solution. By phosgenating this L-lysine ethyl ester dihydrochloride in ortho-dichloro-' benzene for about 8 hours at 100-110°C L-lysine ethyl ester diisocyanate was obtained which was purified by vacuum distillation (boiling point 125°C at 0.1 mm Hg) .

(c) Forming of the polyurethane network

L-lactide/ε-caprolactone copolyester prepolymers were crosslinked by treatment with L-lysine ethyl ester diisocyanate in toluene. The crosslinking of glycolide/ ε-caprolactone copolyester prepolymers was carried out in dichloromethane. The molar ratio of hydroxyl groups to isocyanate groups was 1. By a one-day reaction at room temperature under nitrogen in a Petri dish and a three-hour rehardening at 100-110°C thin films were obtained. The elastic, transparent films were dried at 50°C under reduced pressure.

Porous films having a pore volume of about 85 % are obtained by hardening a viscous suspension of prepolymer, diisocyanate, solvent and an amount of dry NaCl powder having a variable particle size and removing the salt by washing the NaCl polymer blend with water, in the manner as indicated above.

(d) Results

The gel contents (% w/w) were determined by extracting the networks with chloroform. The extracted networks were dried for some days at 50°C under reduced pressure.

Swelling tests were carried out at the extracted networks at room temperature in chloroform. The degree of swelling was calculated from the increase in weight after swelling, using the densities of chloroform ( p - 1.48 g/cτr_3) and the dry extracted networks ( p = 0.90 - 0.95 g/cm³) .

A thermal analysis of the networks was carried out by means of a Perkin-Elmer DSC-7, which was calibrated with reference materials certified by the I.C.T.A. (Internatio Confederation for Thermal Analysis) and was used at a scanning rate of 10°C/min. Mechanical properties were determined at room temperature by means of an Instron (4301) tensile strength tester provided with a ION load cell, at a drawing speed of 12 mm/min. For this purpose, samples of 15 x about 0.75 x about 0.25 mm were cut from extracted or unextracted thin films.

For the porous materials the microstructure was examined by means of an I.S.I.-DS130 scanning electron microscope.

The results of the above-described examinations, with the exception of the electron microscopic examination, are listed in the following Table.

Polyester urethane network data

a⁾ i = myo-inositol/glycolide/ε-caprolactone-prepolymer + L-lysine ethyl ester diisocyanate

2 = extracted network 1

3 = precipitated myo-inositol/glycolide/ε-caprolactone prepolymer + L-lysine ethyl ester diisocyanate

4 = extracted network 3

5 = myo-inositol/L-lactide/ε-caprolactone prepolymer +

L-lysine-ethyl ester diisocyanate

6 = extracted network 5

b⁾ chain length = amount of lactones (L-lactide, glycolide, ε-caprolactone) per OH group, calculated from the employed amount of myo-inositol

^c) in chloroform, 20°C.

Fig. 1 shows the stress-strain curves for the glycolide/ε-caprolactone copolyester urethane networks before (dotted line) and after (solid line) extraction with chloroform, in the concrete the networks 3 and 4 of the Table.

All of the polyurethane networks showed a rubbery behaviour, and the extracted polyurethane networks showed superior mechanical properties, a higher elongation at break and a higher tensile strength (30-40 MPa) when compared with the unextracted networks.

Claims

C L A I M S

1. A biodegradable polyurethane on the basis of a polyol prepolymer and a polyisocyanate, characterized in that the polyisocyanate is an L-lysine derivative having at least 2 isocyanate groups.

2. A polyurethane according to claim 1, in which the polyol prepolymer is a polyester polyol prepolymer.

3. A polyurethane according to claim 1 or 2, in which the L-lysine derivative satisfies the structural formula .

0CN-CH-C00R I (CH₂)₄

NCO in which R is an alkyl, aryl, alkaryl or aralkyl group, which may be substituted by one or more isocyanate groups and/or groups inert in the polyurethane-forming reaction, such as alkoxy groups.

4. A polyurethane according to claim 2 or 3, in which the polyester polyol prepolymer is a polyester diol prepolymer and the L-lysine derivative contains at least 3 isocyanate groups.

5. A polyurethane according to claim 4, in which the

L-lysine derivative is the compound L-lysine aminoethyl

< ester triisocyanate having the structural formula OCN (CH )₂NCO

6. A polyurethane according to claim 4 or 5, in which the polyester diol prepolymer is obtained by a ring-opening polymerization reaction of L-lactide, glycolide and/or one or more lactones, such as ε-caprolactone and δ-valero¬ lactone, if desired with a diol.

7. A polyurethane according to claim 4 or 5, in which the polyester diol prepolymer is obtained by a ring-opening polymerization reaction of L-lactide and/or glycolide with one or more lactones, such as ε-caprolactone and δ-valerolactone, and if desired with a diol.

8. A polyurethane according to claim 2 or 3, in which the polyester polyol prepolymer contains at least 3 hydroxyl groups and the L-lysine derivative is an L-lysine diisocyanate.

9. A polyurethane according to claim 8, in which the L-lysine derivative is the compound L-lysine ethyl ester diisocyanate having the structural formula

10. A polyurethane according to claim 8 or 9, in which the polyester polyol prepolymer is obtained by a ring-opening polymerization reaction of L-lactide, glycolide and/or one or more lactones, such as ε-caprolactone and δ-valero- lactone, with a polyol containing, at- least ;3 hydroxyl. groups.

11. A polyurethane according to claim 8 or 9, in which the polyester polyol prepolymer is obtained by a ring-opening polymerization reaction of L-lactide and/or glycolide with one or more lactones, such as ε-caprolactone and δ-valerolactone, and with a polyol containing at least 3 hydroxyl groups.

12. A polyurethane according to claim 10 or 11, in which the polyol is a cyclic polyol having at least

3 hydroxyl:groups.

13. A pσlyluretharre according to claim 12,. in which the cyclic polyol is myo-inositol.

14. A polyurethane according to claim 1, in which the polyol prepolymer is a polyester polyol prepolymer, obtained by a ring-opening polymerization- reaction of L-lactide or glycolide with ε-caprolactone and with myo-inositol, and the L-lysine polyisocyanate is the compound L-lysine ethyl ester diisocyanate.

15. A polyurethane according to any of the preceding claims consisting of a porous network.

16. A polyurethane according to claim 15, obtainable by carrying out the reaction between polyester polyol prepolymer and L-lysine polyisocyanate in the presence of a salt, such as sodium chloride, and removing this salt later, e.g., by treatment with water, whereby pores remain.

17. A polyurethane according to any of the preceding claims, which comprises precipitating the polyester polyol prepolymer by means of a non-solvent, such as ethanol, from a solution of the prepolymer in an organic solvent, such as chloroform.

18. Products, completely or partly consisting of a polyurethane according to any of the preceding claims.

19. Sheet-like biomedical products, such as artificial skin, wound dressing, etc. and tubular biomedical products, such as artificial veins, vein grafts, nerve grafts etc., completely or partly consisting of a polyurethane according to any of claims 1-17.

20. Biomedical products according to claim 19, comprising one or more layers of a porous polyether urethane as well as one or more layers of a polyurethane according to any of claims 1-17.

21. A polyester polyol prepolymer on the basis of (1) a cyclic polyhydroxy compound having at least 3 functional hydroxyl groups, (2) L-lactide and/or glycolide, and (3) one or more lactones, such as ε-caprolactone and δ-valerolactone.

22. A polyester polyol prepolymer according to claim

21, in which the cyclic polyhydroxy compound is myo-inositol,

23. A polyester polyol prepolymer according to claim 21 or 22, in which the lactone is ε-caprolactone.