WO1987003374A1 - Antibody detection - Google Patents
Antibody detection Download PDFInfo
- Publication number
- WO1987003374A1 WO1987003374A1 PCT/GB1986/000724 GB8600724W WO8703374A1 WO 1987003374 A1 WO1987003374 A1 WO 1987003374A1 GB 8600724 W GB8600724 W GB 8600724W WO 8703374 A1 WO8703374 A1 WO 8703374A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ussn
- virus
- zone
- filter
- reaction zone
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 72
- 239000000463 material Substances 0.000 claims abstract description 15
- 241000700605 Viruses Species 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 241000725303 Human immunodeficiency virus Species 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 7
- 230000002745 absorbent Effects 0.000 claims abstract description 6
- 239000002250 absorbent Substances 0.000 claims abstract description 6
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 2
- 239000004816 latex Substances 0.000 claims description 2
- 229920000126 latex Polymers 0.000 claims description 2
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 208000030507 AIDS Diseases 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000714195 Aids-associated retrovirus Species 0.000 description 1
- 208000008771 Lymphadenopathy Diseases 0.000 description 1
- 241000581002 Murex Species 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- OJYGBLRPYBAHRT-IPQSZEQASA-N chloralose Chemical compound O1[C@H](C(Cl)(Cl)Cl)O[C@@H]2[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]21 OJYGBLRPYBAHRT-IPQSZEQASA-N 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 208000018555 lymphatic system disease Diseases 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/549—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic with antigen or antibody entrapped within the carrier
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Definitions
- This invention relates to detection of antibodies to the AIDS virus.
- AIDS has become a worldwide problem over a short time. The problem is only likely to increase in the foreseeable future. There is apparently no cure at present, but spread of the disease could be contained, e.g. until a cure is found, by quick and accurate diagnosis of potential carriers.
- a subject can usefully be tested by analysis of plasma, serum or another liquid sample, for the presence of antibodies to the virus.
- a summary of the prior art is given in the parent specification.
- SUMMARY OF THE INVENTION A device of the type fully described, claimed and illustrated in the parent specification has AIDS antigen immobilised on the filter material therein and is used for testing for antibodies to the AIDS virus in a liquid sample.
- a filter material has antigen immobilised in a reaction zone thereof, the liquid sample is applied on to the reaction zone and allowed to flow through the filter material where it is taken up by absorbent material disposed in contact with the filter material.
- the device which is preferably used in the invention comprises an aperture through which the specimen can be introduced; a filter including a reaction zone which receives liquid through the aperture, and a peripheral zone; and absorbent means associated with the peripheral zone, but not the reaction zone, of the filter.
- a filter including a reaction zone which receives liquid through the aperture, and a peripheral zone; and absorbent means associated with the peripheral zone, but not the reaction zone, of the filter.
- the specimen is introduced on to the reaction zone of the filter through the aperture, suitably by flow under gravity, and is taken up in the absorbent means/material.
- the filter material By having coated or impregnated the filter material with antigen in the reaction zone, antigen-antibody reaction products remain in the filter, after passage of the specimen and washing as necessary. These products can then be visualised or otherwise tested in conventional manner.
- virus or virus particles are immobilised on latex microparticles or other particles or in the filter material. Owing to the cytotropic nature of AIDS-causing virus or other agent such as HTLV-III, lymphadenopathy virus or AIDS-associated retrovirus, it may be provided on infected T lymphocytes or any other eukaryotic or prokaryotxc cells, or antigen derived from
- Serum under test is then added, possibly containing anti-HTLV, followed by 5 anti-globulin, e.g. anti-human IgG, enzyme-labelled.
- viral DNA e.g. HTLV-DNA
- labelled hydridising DNA can be used to probe DNA exposed by lysis of a clinical sample on the substrate. 10 Washing is conducted as necessary, e.g. between each step as described above. Bound labelled reactant can be visualised in conventional manner, in order to determine the presence of antibody bound to antigen in the reaction zone. 15
Abstract
A process for testing for antibodies to the AIDS virus in a liquid sample, which comprises applying the sample on to a zone of a filter material, the zone having AIDS virus or virus particles immobilised therein, and allowing the liquid to pass through the filter material and to be taken up by absorbent material in contact therewith.
Description
ANTIBODY DETECTION RELATION TO EARLIER APPLICATIONS
This application is related, inter alia, to the following five patent applications:
(1) USA, Serial No. 740,100, filed 3,1st May 1985;
(2) USA, Serial No. 857,914, filed 5th May 1986;
(3) Europe, No. 86304147.1, filed 30th May 1986, published as EP-A-0206561; (4) Canada, No. 510,307, filed 29th May 1986; and (5) Japan, No. (not yet known) , filed ? May 1986. The specifications of 2, 3, 4 and 5 (including the drawings) are essentially the same and are incorporated herein by reference; that subject matter is hereinafter described as "the parent specification". 3 and 5 are filed in the name of Murex Corporation, claiming priority from 1 and 2. Dinesh Andrew Jacob and Miles Gerald
Hossom are named as the inventors.
FIELD OF THE INVENTION This invention relates to detection of antibodies to the AIDS virus.
BACKGROUND OF THE INVENTION
AIDS has become a worldwide problem over a short time. The problem is only likely to increase in the foreseeable future. There is apparently no cure at present, but spread of the disease could be contained, e.g. until a cure is found, by quick and accurate diagnosis of potential carriers.
A subject can usefully be tested by analysis of plasma, serum or another liquid sample, for the presence of antibodies to the virus.
PRIOR ART
A summary of the prior art is given in the parent specification. SUMMARY OF THE INVENTION
A device of the type fully described, claimed and illustrated in the parent specification has AIDS antigen immobilised on the filter material therein and is used for testing for antibodies to the AIDS virus in a liquid sample.
In general terms, a filter material has antigen immobilised in a reaction zone thereof, the liquid sample is applied on to the reaction zone and allowed to flow through the filter material where it is taken up by absorbent material disposed in contact with the filter material. DETAILED DESCRIPTION OF THE INVENTION
The device which is preferably used in the invention comprises an aperture through which the specimen can be introduced; a filter including a reaction zone which receives liquid through the aperture, and a peripheral zone; and absorbent means associated with the peripheral zone, but not the reaction zone, of the filter. Such a device is fully described and illustrated in the parent specification.
In a process according to the invention, the specimen is introduced on to the reaction zone of the filter through the aperture, suitably by flow under gravity, and is taken up in the absorbent means/material. By having coated or impregnated the filter material with antigen in the reaction zone, antigen-antibody reaction products remain in the filter, after passage of the specimen and washing as necessary. These products can then be visualised or otherwise tested in conventional manner.
By way of example, virus or virus particles are immobilised on latex microparticles or other particles or in the filter material. Owing to the cytotropic nature of AIDS-causing virus or other agent such as HTLV-III, lymphadenopathy virus or AIDS-associated retrovirus, it
may be provided on infected T lymphocytes or any other eukaryotic or prokaryotxc cells, or antigen derived from
A gene-cloning, which are immobilised. Serum under test is then added, possibly containing anti-HTLV, followed by 5 anti-globulin, e.g. anti-human IgG, enzyme-labelled.
Alternatively, viral DNA, e.g. HTLV-DNA, is used, and can be probed with complementary labelled DNA. Again, labelled hydridising DNA can be used to probe DNA exposed by lysis of a clinical sample on the substrate. 10 Washing is conducted as necessary, e.g. between each step as described above. Bound labelled reactant can be visualised in conventional manner, in order to determine the presence of antibody bound to antigen in the reaction zone. 15
20
25
30 *s
35
Claims
1. A process for testing for antibodies to the AIDS virus in a liquid sample, which comprises applying the sample on to a zone of a filter material, the zone having AIDS virus or virus particles immobilised therein, and allowing the liquid to pass through the filter material and to be taken up by absorbent material in contact therewith.
2. A process according to claim 1, which comprises introducing, after the specimen, a labelled material which binds with the components in the specimen which have been immobilised on the filter.
3. A device which comprises an aperture through which the specimen can be introduced; a filter including a reaction zone which receives liquid through the aperture, and a peripheral zone; and absorbent means associated with the peripheral zone, but not the reaction zone, of the filter; and in which AIDS virus or virus particles are immobilised in the reaction zone.
4. A process or device according to any preceding claim, wherein the virus or virus particles are carried on latex or other particles or on T-lymphocytes.
5. A process or device according to any of claims 1 to
4. wherein the device is as defined in claim 1 of USSN 857,914 or EP-A-0206561.
6. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 2 of USSN 857,914 or EP-A-0206561.
7. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 3 of USSN
857,914 or EP-A-0206561.
8. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 4 of USSN 857,914 or EP-A-0206561.
9. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 5 of USSN 857,914 or EP-A-0206561.
10. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 6 of USSN
_ 857,914 or EP-A-0206561.
11. A process or device according to any of claims 1 to 5 4, wherein the device is as defined in claim 7 of USSN
857,914 or EP-A-0206561.
12. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 8 of USSN 857,914 or EP-A-0206561.
10 13. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 9 of USSN 857,914 or EP-A-0206561.
14. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 10 of USSN
15 857,914 or EP-A-0206561.
15. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 11 of USSN 857,914 or EP-A-0206561.
16. A process or device according to any of claims 1 to 20 4, wherein the device is as defined in claim 12 of USSN
857,914 or EP-A-0206561.
17. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 13 of USSN 857,914 or EP-A-0206561.
25 18. A process or device according to any of, claims 1 to
4, wherein the device is as defined in claim 14 of USSN
857,914 or EP-A-0206561.
19. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 15 of USSN 30 857,914 or EP-A-0206561. '""
20. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 16 of USSN
857,914 or EP-A-0206561.
21. A process or device according to any of claims 1 to 35 4, wherein the device is as defined in claim 17 of USSN
857,914 or EP-A-0206561.
22. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 18 of USSN 857,914 or EP-A-0206561.
23. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 19 of USSN
857,914 or EP-A-0206561.
24. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 20 of USSN 857,914 or EP-A-0206561.
25. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 21 of USSN 857,914 or EP-A-0206561.
26. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 22 of USSN 857,914 or EP-A-0206561.
27. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 23 of USSN 857,914 or EP-A-0206561.
28. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 24 of USSN
857,914 or EP-A-0206561.
29. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 25 of USSN 857,914 or EP-A-0206561.
30. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 26 of USSN 857,914 or EP-A-0206561.
31. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 27 of USSN 857,914 or EP-A-0206561.
32. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 28 of USSN 857,914 or EP-A-0206561.
33. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 29 of USSN
857,914 or EP-A-0206561.
34. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim- 30 of USSN 857,914.
35. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 31 of USSN
857,914.
36. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 32 of USSN 857,914.
37. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 33 of USSN 857,914.
38. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 34 of USSN 857,914.
39. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 35 of USSN 857,914.
40. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 36 of USSN
857,914.
41. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 37 of USSN 857,914.
42. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 38 of USSN 857,914.
43. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 39 of USSN 857,914.
44. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 40 of USSN 857,914.
45. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 41 of USSN
857,914.
46. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 42 of USSN 857,914.
47. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 43 of USSN
857,914.
48. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 44 of USSN 857,914.
49. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 45 of USSN 857,914.
50. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 46 of USSN 857,914.
51. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 47 of USSN 857,914.
52. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 48 of USSN
857,914.
53. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 49 of USSN 857,914.
54. A process or device according to any of claims 1 to
4, wherein the device is as defined in claim 50 of USSN 857,914.
55. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 51 of USSN 857,914.
56. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 52 of USSN 857,914.
57. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 53 pf USSN
857,914.
58. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 54 of USSN 857,914.
59. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 55 of USSN
857,914.
60. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 56 of USSN 857,914.
61. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 57 of USSN 857,914.
62. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 58 of USSN 857,914.
63. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 59 of USSN 857,914.
64. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 60 of USSN
857,914.
65. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 61 of USSN 857,914.
66. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 62 of USSN 857,914.
67. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 63 of USSN 857,914.
68. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 64 of USSN 857,914.
69. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 65 of USSN
857,914.
70. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 66 of USSN 857,914.
71. A process or device according to any of claims 1 to 4, wherein the device is as defined in claim 67 of USSN
857,914.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8529067 | 1985-11-26 | ||
GB858529067A GB8529067D0 (en) | 1985-05-31 | 1985-11-26 | Diagnosis |
GB858529276A GB8529276D0 (en) | 1985-11-28 | 1985-11-28 | Diagnosis |
GB8529276 | 1985-11-28 | ||
US06/857,914 US4693834A (en) | 1986-05-05 | 1986-05-05 | Transverse flow diagnostic kit |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1987003374A1 true WO1987003374A1 (en) | 1987-06-04 |
Family
ID=27262851
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1986/000724 WO1987003374A1 (en) | 1985-11-26 | 1986-11-26 | Antibody detection |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0247124A1 (en) |
WO (1) | WO1987003374A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988010272A1 (en) * | 1987-06-26 | 1988-12-29 | Garth Alexander Nicholson | Method of preserving a body fluid sample, prior to assay for hiv (aids) infection |
US5077198A (en) * | 1988-04-14 | 1991-12-31 | Eastman Kodak Company | Diagnostic kit and method for rapid detection of antibodies |
US5268299A (en) * | 1988-04-14 | 1993-12-07 | Eastman Kodak Company | Diluent composition useful in the detection of antibodies in assays |
US5759774A (en) * | 1988-05-18 | 1998-06-02 | Cobe Laboratories, Inc. | Method of detecting circulating antibody types using dried or lyophilized cells |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0119623A2 (en) * | 1983-03-17 | 1984-09-26 | Fuji Photo Film Co., Ltd. | Multilayer analytical element for non-isotopic assay |
US4520113A (en) * | 1984-04-23 | 1985-05-28 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions |
EP0173295A1 (en) * | 1984-08-31 | 1986-03-05 | New York Blood Center, Inc. | Assay for simultaneous detection of antigen and antibody in given serum |
-
1986
- 1986-11-26 WO PCT/GB1986/000724 patent/WO1987003374A1/en not_active Application Discontinuation
- 1986-11-26 EP EP86906897A patent/EP0247124A1/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0119623A2 (en) * | 1983-03-17 | 1984-09-26 | Fuji Photo Film Co., Ltd. | Multilayer analytical element for non-isotopic assay |
US4520113A (en) * | 1984-04-23 | 1985-05-28 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions |
EP0173295A1 (en) * | 1984-08-31 | 1986-03-05 | New York Blood Center, Inc. | Assay for simultaneous detection of antigen and antibody in given serum |
Non-Patent Citations (2)
Title |
---|
Analytical Chemistry, Volume 57, No. 7, June 1985, American Chemical Society, (Washington, DC, US), M.D.W.: "AIDS Antibody Screening Test", see pages 773A-778A * |
Biotechnology, Volume 3, No. 10, October 1985, (New York, US), t.W. CHANG et al.: "Detection of Antibodies to Human T-Cell Lymphotropic Virus-III (HTLV-III) with an Immunoassay Employing a Recombinant Escherichia Coli-Derived Viral antigenic Peptide", pages 905-909, see page 908 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988010272A1 (en) * | 1987-06-26 | 1988-12-29 | Garth Alexander Nicholson | Method of preserving a body fluid sample, prior to assay for hiv (aids) infection |
US5077198A (en) * | 1988-04-14 | 1991-12-31 | Eastman Kodak Company | Diagnostic kit and method for rapid detection of antibodies |
US5268299A (en) * | 1988-04-14 | 1993-12-07 | Eastman Kodak Company | Diluent composition useful in the detection of antibodies in assays |
US5759774A (en) * | 1988-05-18 | 1998-06-02 | Cobe Laboratories, Inc. | Method of detecting circulating antibody types using dried or lyophilized cells |
Also Published As
Publication number | Publication date |
---|---|
EP0247124A1 (en) | 1987-12-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0267317B1 (en) | Method for the detection of proteins and viruses | |
US5571667A (en) | Elongated membrane flow-through diagnostic device and method | |
AU614109B2 (en) | Test method and reagent kit therefor | |
EP0595641A2 (en) | One-step simultaneous immunoassay | |
EP0139389A1 (en) | Antiidiotypic monoclonal antibody reagents and immunoassays employing antiidiotypic monoclonal antibody reagents | |
PL196464B1 (en) | Neutralisation of polycations in chromatographic equipment used to analyse whole blood | |
JP3693680B2 (en) | Assays using magnetic particles | |
JPS6126860A (en) | Method and device for executing analysis measurement | |
CA2137786A1 (en) | Immunoassay | |
JP3908272B2 (en) | Solid phase assay for detection of ligands | |
EP2796880B1 (en) | Platelet allo-antigen typing and platelet antibody tests | |
CA1170180A (en) | Process for the enzyme immuno determination in the heterogenous phase | |
WO1987003374A1 (en) | Antibody detection | |
EP0402023A1 (en) | Elongated membrane flow-through diagnostic device and method | |
WO1987003373A1 (en) | Virus detection | |
Olaussen et al. | Screening tests for antinuclear antibodies (ANA): selective use of central nuclear antigens as a rational basis for screening by ELISA | |
JP2549305B2 (en) | Independent multiplex immunoassay diagnostic system | |
AU2015213838B2 (en) | Crossmatching blood samples | |
CN218331595U (en) | IgG antibody subtype detection kit | |
JP3618797B2 (en) | Immunoassay | |
CA2056278C (en) | Washing solution, which contains a complexing agent for metal ions, for a solid-phase immunometric method, and the use thereof | |
JPH08114595A (en) | Specific bonding measuring method | |
JPH06281652A (en) | Detecting method of antibody and antigen | |
JPH02151769A (en) | Reaction container | |
JPH08240592A (en) | Method for preventing non-specific reaction in immunological inspection |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): JP US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE FR GB IT LU NL SE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1986906897 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1986906897 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1986906897 Country of ref document: EP |