WO1985002189A1

WO1985002189A1 - 1,25-dihdroxyvitamin d2 compounds and methods for their preparation

Info

Publication number: WO1985002189A1
Application number: PCT/US1984/001334
Authority: WO
Inventors: Hector F. Deluca; Heinrich K. Schnoes; Rafal R. Sicinski; Yoko Tanaka
Original assignee: Wisconsin Alumni Research Foundation
Priority date: 1983-11-07
Filing date: 1984-08-20
Publication date: 1985-05-23
Also published as: FR2554444A1; FR2554444B1; BE900979A; GB2149408A; GB8428031D0; US4769181A; AU3390284A

Abstract

Preparation of hydroxylated compounds of the vitamin D2 series and specifically to a process for synthesizing 1alpha,25-dihydroxy vitamin D2, 1beta,25-dihydroxyvitamin D2, their corresponding 5,6-trans isomers and the C-24 epimers of these compounds. The hydroxylated vitamin D2 compounds obtained exhibit vitamin D-like activity and can be substituted for vitamin D3 or various of its known metabolites where such compounds are applied.

Description

1,25-Dihydroxyvitamin D₂ Compounds and Methods for Their Preparation

This invention was made with Government support under NIH Grant No. AM 14881 awarded by the Department of Health and Human Services. The Government has certain rights in this invention. Technical Field

This invention relates to the preparation of nydroxylated compounds of the vitamin D₂ series.

More specifically, this invention relates to a process for the synthesis of 1α,25-dihydroxyvitamin D₂ and 1β,25- dihydroxyvitamin D₂, of the corresponding 5 ,6-trans-isomers, and the C-24 epimers of these compounds. Background Art The importance of hydroxylated forms of vitamin D as regulators of calcium and phosphate metabolism in animals and humans is by now well established through many disclosures in the patent and general literature. Vitamin D₃, the natural form of the vitamin produced in skin, is known to be hydroxylated in vivo to 25-hyάroxyvitamin D₃ and then to 1α,25-dihydroxyvitamin D₃, the latter compound being generally regarded as the tissue-active hormonal form of the vitamin. Likewise, vitamin D₂, which is comnonly used as a food additive or vitamin D supplement, undergoes the same hydroxylation sequence in vivo to form 25-hydroxyvitamin D₂ (25-OH-D₂) and 1α,25-dihydroxyvitamin D₂ (1α,25-(OH)₂D₂) , the latter being essentially as active as 1α,25-diiιydroxyvitamin D₃ in humans and other mammals.

Both 25-OH-D₂ and 1α,25-(OH)₂D₂, the structures of which are shown below, have been isolated. and characterized as metabolites of vitamin D₂ (DeLuca et al. , U.S. patents 3,585,221; 3,880,894) .

25-OH-D₂ 1α,25-(OH) ₂D₂

These metabolites, being derived from, vitamin D₂ , are characterized by the S-stereochemistry at carbon 24, as shown above. Disclosure of Invention

A process for the preparation of 1,25-dihydroxyvitamin D₂ ccmpounds has now been developed. The process is notable in that, in addition to the 5,6-cis- and trans-1α,25-dihydroxy vitamin D₂ compounds, it also provides 1β,25-dihydroxyvitamin D₂ compounds and the corresponding 5,6-trans isomers. These latter analogs are of particular interest because of their unexpectedly potent biological properties.

The synthetic process is outlined in Scheme I. Specific compound designations by Arabic numeral or letter (e.g. 1, 2, . . . 6a, 6b, etc.) as used in the following description or the Examples refer to the structures so numbered in Scheme I or in the body of this disclosure. A wavy line drawn to a substituent in these structures, e.g. as in the case of the substituent at carbons 1 and 24, indicates that such substituent may have either of the two possible stereochemical configurations. Best Mode for Carrying out the Invention

A convenient starting material for this process is 25-hydroxyvitamin D₂ (structure 1, 24S-stereochemistry), or its 24R-epimer, 25-hydroxy-24-epi-vitamin D₂ (structure 1, 24R-stereochemistry), or a mixture of both compounds. Both Process Scheme I

25-hydroxyvitamin D₂, having the natural 24S-configuration, and its 24R-epimer ia a known compound. The 24R-epimer has been prepared previously (DeLuca, U.S. 3,585,221 and DeLuca et al. U.S. Patent Application Serial No. 420,191, filed September 20, 1982) . For the purpose of the present process it is convenient to use a mixture of the 24R and S-epimers as starting material, epimer separation being accomplished at a later stage (as described below) . It is important to note, however, that the synthetic process can be executed equally well and in an entirely analogous fashion with the pure 24R or 24S-epimer of compound 1 as starting material, whereby, of course, the use of the 24R-epimer would yield specifically the 24R-epimer of the 1,25-dihydroxyvitamin D₂ product, whereas the use of the 24S-epimer would yield the corresponding 24S-1,25-dihydroxyvitamin D₂ product.

The first step of the process comprises conversion of starting material of structure 1 to the corresponding C-3-tosylate (structure 2) using conventional tosylation procedures, and subsequent solvolysis of the tosylate according to the procedures of DeLuca et al. (U.S. Patent 4,195,027) to obtain the cyclovitamin D derivative of structure 3. This intermediate is then subjected to allylic hydroxylation with SeO₂/t-butyl hydroperoxide, to obtain the 1-hydroxy-cyclovitamin D₂ derivatives. This allylic hydroxylation process yields the 1α-hydroxy-cyclovitamin D product represented by structure 4 as expected, but also, in this case, the corresponding 1β-hydroxy isomer of structure 5. Formation of the 1β-hydroxy-isomer in this reaction was unexpected, since in previous work (U.S. Patent 4,195,027) only the 1α-hydroxy product had been recovered. The 1β-hydroxy compound 5 is the minor component of the product mixture. If desired, isomer separation can be accomplished by conventional methods, e.g. high performance chrcmatography, but for the purposes of the present process, isomer separation is not required at this stage. Direct solvolysis of this 1-hydroxy-cyclovitamin D₂ product mixture (compounds 4 and 5) in a medium containing a low-molecular weight organic acid, then provides, in admixture, the desired 5,6-cis- and 5,6-trans-1,25-dihydroxyvitamin D₂ compounds as the 3-O-acylates, namely the compounds of structures 6a/b and the corresponding 5,6-trans-isomers (structure 8a/b) and the 1β-hydroxy-epimers of structures 7a/b as well as their corresponding 5,6-trans-isomers (9a/b) . After initial fractionation of this mixture by conventional high performance liquid chrcmatography, followed by mild base hydrolysis of each fraction (to remove the acyl groups) and subsequent further separation and purification of products by high performance chrcmatography, it is possible to obtain any or all of the following compounds in isolated form: 1α,25-dihydroxyvitamin D₂ (10a) , the natural product having the 24S-stereochemistry), the corresponding (24R)-epimer, 1α,25-dihydroxy-24-epivitamin D₂ (10b) , 5,6-trans-1α,25- dihydroxyvitamin D₂ (12a) , the corresponding (24R) -epimer (12b) , as well as 1β,25-dihydroxyvitamin D₂ (11a; 24S-stereochemistry) and its (24R) -epimer, 1β,25-dihydroxy-24-epi-vitamin D₂ (compound 11b) , and the corresponding 5, 6-trans-isomers 13a and 13b.

In the present case, compounds 10a, 10b, 11a, 11b, 12a and 12b were recovered by chromatographic separation of the reaction mixture. The 5,6-trans compounds 13a and 13b can also be isolated from the solvolysis mixture by careful chrcmatography, but when their abundance in the mixture is low so as to make recovery tedious and time-consuming, it is generally more convenient to obtain them from the cis-isomers 11a and 11b by 5,6-double bond isomerization.

Thus, treatment of the 5,6-cis isomer 11a with iodine, according to the conventional procedure, gave upon chromatographic purification, the 5,6-trans isomer 13a; compound 11b was likewise converted to its trans isomer 13b. In order to securely establish the C-24 stereochemistry of the novel 1β,25-dihydroxyvitamin D₂ compounds, these 1β-isomers were also chemically correlated with the known 1α-hydroxy compounds by the following procedure. Treatment of 1α,25-dihydroxyvitamin D₂ (10a) with manganese dioxide, gave the corresponding 1-oxo-25-hydroxyprevitamin D₂ compound of structure 14a, shown below:

14a (24S) 14b (24R)

Upon reduction of this compound with LiAlH₄, and subsequent thermal isomerization of the previtamin chromophore to the vitamin D triene system, there was obtained 1β,25-dihydroxyvitamin D₂ (structure 11a) . Like treatment of 1α,25-dihydroxy-24-epivitamin D₂ (structure 10b) gave ketone 14b (above) and then 1β,25-dihydroxy-24-epivitamin D₂ (structure 11b) . These interconversions, and the cis to trans conversions (11a,b to 13a,b) described earlier, relate all four 1β,25-dihydroxyvitamin D compounds with the corresponding 1α-hydroxy-isomers and thus establish the C-24 stereochemistry for all compounds.

Although for therapeutic applications, the free hydroxy compounds represented by structures 10, 11, 12 and 13 are generally used, for some such applications, the corresponding hydroxy-protected derivatives may be useful or preferred. Such hydroxy-protected derivatives are for example the acylated compounds represented by general formulae 15 and 16 below,

wherein each of R₁, R₂, and R₃ is selected from the group onsisting of hydrogen and acyl, except that at least one of R₁, R₂ and R₃ must be acyl. The term 'acyl' refers to an aliphatic acyl group (alkanoyl group) of from 1 to 6 carbons, in all possible isomeric forms (e.g. formyl, acetyl, butyryl, isobutyryl, valeryl, etc.) or an aromatic acyl group (aroyl group) , such as benzoyl, or the methyl- halo- or nitre- substituted benzoyl groups, or a dicarboxylic acyl group of from 2 to 6 atoms chain length, i.e. acyl groups of the type ROOC(CH₂)_nCO-, or RCOCCH₂-O-CH₂CO-, where n has values between 0 and 4 inclusive, and R is hydrogen or an alkyl radical. Representative of such dicarboxylic acyl groups are oxalyl, malonyl, succinoyl, glutaryl, adipyl and diglycolyl. The term 'alkyl' refers to a lower alkyl group of 1 to 6 carbons in all possible isomeric forms, e.g. methyl, ethyl, propyl, isopropyl, isobutyl, pentyl, etc.

Such acyl derivatives are conveniently prepared from the free hydroxy compounds (or, if desired, from the C-3-monoacylated intermediates of structure 6, 7, 8 or 9) by conventional acylation procedures, i.e. treatment of any of the hydroxyvitamin D₂ products with an acyl chloride, or acyl anhydride in a suitable solvent such as pyridine, or alkylpyridine. By appropriate selection of reaction time, acylating agent, temperature and solvent, as is well-known in the art, the partially or fully acylated derivatives represented by structures 15 or 16 above are obtained. For example, treatment of 1β,25-dihydroxyvitamin D₂ in pyridine solvent with acetic anhydride at room temperature gives the 1,3-diacetate (15, R₁=R₂=acetyl, R₃=H) , while the same reaction conducted at elevated temperature yields the corresponding 1,3,25-triacetate. The 1,3-diacetate can be further acylated at C-25 with a different acyl group; e.g. treatment with benzoyl chloride or succinic anhydride would provide the 1,3-diacetyl-25-benzoyl-, or β,3-diacetyl-25-succinoyl-derivative, respectively. A 1,3,25-triacyl derivative can be selectively hydrolyzed in mild base to provide the 1,3-dihydroxy-25-acyl compound, the free hydroxy groups of which can be reacylated, if desired, with different acyl groups. Likewise, a 1,3-diacyl derivative can be subjected to partial acyl hydrolysis to obtain the 1-O-acyl and the 3-O-acyl-compounds, which in turn can be reacylated with different acyl groups. Like treatment of the other hydroxyvitamin D₂ products (10, 11, 12 or 13) provides the corresponding desired acyl derivatives of structures 15 or 16.

Like the previously known vitamin D₂ metabolite, 1α,25-dihydroxyvitamin D₂ (10a) , the novel compounds of this invention exhibit pronounced vitamin D-like activity, and thus represent desirable substitutes for the known vitamin D₂ or D₃ metabolites in many therapeutic or veterinary applications. Particularly preferred in this regard are the products of structure 13a and 13b. These compounds exhibit high binding affinity for the intestinal receptor protein, and since binding affinity generally correlates with high in vivo activity, these compounds can be expected to be especially useful for the treatment of diseases related to mineral imbalance. The novel compounds may be used for correcting or improving a variety of calcium and phosphate imbalance conditions resulting from a variety of diseases, such as vitamin D-resistant rickets, osteomalacia, hypoparathyroidism, pseudohypoparathyroidism, osteoporosis, Paget's disease, and similar bone and mineral-related disease states well known to the medical practice. The compounds can also be used for the treatment of mineral imbalance conditions in animals, such as the milk fever condition, poultry or swine leg weakness, or for improving egg shell quality of fowl.

For therapeutic purposes, these compounds may be administered orally or by injection or infusion in any form convenient or appropriate to the method of administration selected. Thus the compounds may be formulated with any therapeutically acceptable and innocuous carrier, in the form of pills, tablets or gelatin capsules for oral administration, or they may be formulated as solutions, emulsions, dispersions or suspensions in innocuous solvents and oils, and such formulations may contain also other therapeutically active and beneficial constituents, such as other vitamins, salts, sugars, hormones, etc. as may be appropriate to the specific application. The novel compounds may be admnistered singly or as mixtures, e.g. mixtures of 13a and 13b, or mixtures of 11a, 11b, 13a, 13b, or any other combination of the individual stereoisomers of this invention. Advantageously, the compounds of this invention are administered in dosage amounts of between about 0.5 to 100 μg per day, it being understood, of course, that the specific dosage administered in any given case will be adjusted in accordance with the specific compound administered, the disease to be treated, the condition of the subject and other relevant medical facts that may modify the activity of the drug or the response of the subject, as is well-known by those skilled in the art. Example 1

Preparation of (24R/S-1,25-dihydroxy-3,5-cyclovitamin D₂ (compounds 4 and 5) :

Freshly recrystallized p-toluenesulfonyl chloride (50 mg) was added to a solution of 33 mg (24R/S)-25-hydroxyvitamin D₂ (1) in dry pyridine (300 μl) . The reaction mixture was allowed to stand for 30 hours at 4°C, poured into ice/saturated NaHCO₃ with stirring and extracted with benzene. The combined extracts were washed with NaHCO₃, water, aqueous CuSO₄ solution, water, then dried over MgSO₄. Removal of solvent under reduced pressure gave a crude tosylate (2) , which can be used directly for the next reaction.

A mixture of product (2) and NaHCO₃ (150 mg) in 10 ml of anhydrous methanol was heated at 55°C for 8.5 h with stirring, concentrated to Ca. 2 ml and diluted with 80 ml of benzene. The benzene solution was washed with water, dried over MgSO₄ and evaporated. The oily product (3) was sufficiently pure for the following oxidation step.

To a stirred suspension of 4 mg of SeO₂ in 5 ml of dry CH₂Cl₂ was added 13.2 μl of t-BuOOH. After 0.5 h, 40 μl of anhydrous pyridine was added and the mixture was stirred at room temperature until homogeneous. Dichlorαnethane (3 ml) was then added, the solution was cooled to 0°C and the cyclcvitamin product (3) was added in 4.5 ml of CH₂Cl₂. After 15 min, the reaction was allowed to warm slowly to room temperature and continued until almost all starting material was consumed (Ca. 30 min) . The mixture was transferred to a separating funnel and shaken with 30 ml of 10% NaOH. Ether (150 ml) was added and the phases were separated. The etheral phase was washed with 10% NaOH, water, dried over MgSO₄ and evaporated in vacuo. The oily residue was purified by preparative TIC (developed with 6:4 n-hexane-ethyl acetate) . The isolated product (12 mg) represents a mixture containing 1α-hydroxyclovitamin 4 and a lesser amount of the corresponding 1β-hydroxy derivative 5, and is characterized by the following physical data: mass spectrum, m/e 442 (M⁺, 13) , 424 (8), 410 (9), 392 (26), 352 (15), 269 (27), 135 (88), 59 (100); NMR (CDCl₃) δ 0.55 (3H, s, 18-H₃) , 0.63 (1H, m, 3-H), 1.00 (3H, d, J=6.5 Hz, 28-H₃) , 1.05 (3H, d, J=6.5 Hz, 21-H₃) , 1.13 and 1.18 (6H, each s, 26-H₃ and 27-H₃), 3.26 (3H, s, 6-OCH₃), 4.19 (1H, d, J=9.5 Hz, 6-H) , ~4.2 (1H, m, 1-H), 4.96 (1H, d, J=9.5 Hz, 7-H), 5.17 and 5.24 (2H, each m, 19-H₂) , 5.35 (2H, broad m, 22-H and 23-H) . Example 2 a) Cycloreversion of 1-hydroxycyclovitamins 4 and 5

A solution containing a mixture of 1-hydroxycyclovitamins 4 and 5 (6 mg) in glacial acetic acid (0.5 ml) was heated at 55°C for 15 min, cooled and poured carefully over ice- saturated NaHCO₃. The mixture was extracted with benzene and ether, and the combined extracts were washed with saturated NaHCO₃ and water. The residue was chrcrratographed on a HPLC column (6.2 mm x 25 cm Zorbax-Sil) using 3% 2-propanol in hexane as eluent. Chrcmatography yielded a fraction (1.4 mg) containing 1α,25-dihydroxyvitamin D₂3-acetate (6a) and both 24-epimers of the corresponding 1β,25-dihydroxy derivative 7 (peak collected at 90 ml) as well as a fraction (2.5 mg) containing of (24R)-1α,25-dihydroxy- vitamin D₂3-acetate (6b) and both 24-epimers of the 5,6-trans-1α,25-dihydroxyvitamin 8 (peak collected at 97 ml; 1:1 ratio at 6b and 8 was established by NMR) . b) Hydrolysis of 3β-acetoxy group

A solution of the mixture containing 6a and 7 (1.1 mg) as obtained above in 10% methanolic NaOH (1 ml) was heated at 55°C for 1 hour, then poured into the water and extracted with benzene, ether and methylene chloride. The organic extracts were washed with water, dried, combined and evaporated. HPLC of the residue (10% 2-propanol/hexane, 6.2 x 25 cm Zorbax-Sil column) afforded a mixture of 24-epimers of 1β,25-dihydroxyvitamin D₂11 (0.15 mg, peak collected at 52 ml) and a pure 1α,25-dihydroxyvitamin D₂10a (0.6 mg, peak collected at 59 ml). Rechromatography of 11 was performed on HPLC (4.6 mm x 25 cm Zorbax-Sil column using 4% 2-propanol in hexane as an eluent). Pure compounds 11a and 11b were collected at 90 and 97 ml.

Ey analogous treatment of the mixture containing 6b and 8 (2.4 mg) as obtained above with NaOH a mixture of (24R)-1α,25-dihydroxyvitamin D₂ (10b) and 24-epimers of 5,6-trans-1α,25- dihydroxyvitamin 12 (1.7 mg, 1:1 ratio of 10b and 12) was obtained. Separation of the isomers was achieved on HPLC (4.6 x 25 cm Zorbax-Sil column, 6% 2-propanol/hexane) . The chrcsratographic peaks for (24S)-5,6-trans-1α,25-dihyd_oxyvitamin D₂ (12a), (24R)-1α,25-dihydroxyvitamin D₂ (10b) and (24R)-5,6-trans-1α,25-dihydroxyvitamin D₂ (12b) were partially overlapped (57 ml, 59 ml and 62 ml respectively) but recycling afforded pure compounds. c) Cis to Trans Isomerization

The 5,6-cis form of the 1,25-dihydroxyvitamin D compounds thus obtained can be converted to the trans compounds by treatment with iodine. Thus, treatment of compound 10a in ether with a catalytic amount of iodine (2% of the amount of 10a) , while keeping the solution under diffuse daylight for 1 hr, results in cis to trans isomerization, and after HPLC purification (Zorbax-Sil column, 4.6 x 25 cm), 6% 2-propanol/hexane), the 5,6-trans-isomer 12a was obtained. Under like conditions, compound 10b is isomerized to 12b. Similarly, compound 11a, upon treatment with iodine under the above conditions provided a mixture of the 5,6-cis and 5,6-trans-isomer (11a, 13a) which when separated by HPLC (Zorbax-Sil, 9.6 x 25 cm, 10% 2-propanol/hexane) gave 13a in pure form, and treatment of compound 11b under the same conditions, gave the 5,6-trans compound 13b. These cis to trans conversions are useful for correlating the respective C-24-epimers of the major 5,6-cis products with the minor 5,6-trans compounds resulting from the above solvolysis. Also, if the quantity of starting material used in the above solvolysis reaction is low, so as to make purification of the minor trans isomers from the solvolysis mixture inefficient or unduly time consuming, the cis to trans conversion may be used for preparing quantities of pure trans material.

The following spectral data characterize the products obtained: 1α,25-dihydroxyvitamin D₂ (10a): UV (EtOH) λ_max 265.5 nm, λ_min 227.5 nm; mass spectrum, m/e 428 (M⁺ , 6) , 410 (4) ,

352 (4), 287 (6), 269 (10), 251 (10), 152 (42), 134 (100), 59

(99); NMR (CDCl₃) δ 0.56 (3H, s, 18-H₃) , 1.01 (3H, d, J=6.5

Hz, 28-H₃), 1.04 (3H, d, J=6.5 Hz, 21-H₃) , 1.14 and 1.18 (6H, each s, 26-H₃ and 27-H₃) , 4.24 (1H, m, 3-H) , 4.43 (1H, m,

1-H), 5.01 (1H, m, 19-H), 5.34 (3H, broad m, 19-H, 22-H and

23-H), 6.02 (1H, d, J=11 Hz, 7-H), 6.39 (1H, d, J=11 Hz, 6-H). 1α,25-dihydroxy-24-epivitamin D₂ (10b): UV (EtOH) λ_max

265.5 nm, λ_min 227.5 nm; mass spectrum, m/e 428 (M⁺, 13) , 410

(9), 352 (7), 287 (11), 269 (15), 251 (13), 152 (52), 134 (100), 59 (97).

1β,25-dihydroxyvitamin D₂ (11a): UV (EtOH) λ_max 263.5 nm, λ_min 227 nm; mass spectrum, m/e 428 (M⁺, 9) , 410 (27) , 392

(12), 352 (8), 334 (7), 269 (12), 251 (15), 152 (48), 135 (68), 134 (53), 59 (100).

1β,25-dihydroxy-24-epivitamin D₂ (11b) : UV (EtOH) λ_max

263.5 nm, λ_min 227 nm; mass spectrum, m/e 428 (M⁺, 10) , 410

(29), 392 (13), 352 (9), 334 (7), 269 (13), 251 (16), 152 (58), 135 (76), 134 (59), 59 (100).

5,6-trans-1α,25-dihydroxyvitamin D₂ (12a): UV (EtOH) λ_max 273.5 nm, λ_min 230 nm; mass spectrum, m/e 428 (M⁺, 8),

410 (3) , 287 (3) , 269 (7) , 251 (7) , 152 (34) , 134 (100) , 59

(78) .

5,6-trans-1α,25-dihydroxy-24-epivitamin D₂ (12b) : UV

(EtOH) λ_max 273.5 nm, λ_min 230 nm; mass spectrum, m/e 428 (M⁺ ,

10) , 410 (4) , 352 (4) , 287 (5) , 269 (9) , 251 (8) , 152 (37) , 134 (100) , 59 (82) .

5, 6-trans-1β, 25-dihydroxyvitamin D₂ (13a) : UV (EtOH) λ_max 270 nm, λ_min 229.5 nm; mass spectrum, m/e 428 (11) , 410

(5) , 351 (4) , 287 (4) , 269 (12) , 251 (11) , 152 (67) , 135 (100) , 134 (75) , 59 (72) .

5,6-trans-1β,25-dihydroxy-24-epivitamin D₂ (13b) : UV

(EtOH) λ_max 270 nm, λ_min 229.5 nm; mass spectrum, m/e 428 (M⁺ , 11), 410 (4), 351 (4), 287 (4), 269 (13), 251 (12), 152 (64), 135 (100), 134 (70), 59 (63). Example 3

Conversion of 1α,25-dihydroxyvitamins D₂ (10a,b) to 1β,25-dihydroxyvitamin D₂ analogs (11a,b) :

A solution of 1α,25-dihydroxyvitamin D₂10a (40 μg) in anhydrous ether (1 ml) was treated with activated MnO₂ (6 mg) for 5 h at room temperature. The mixture was filtered

(Celite) , evaporated and the residue was chromatographed on

HPLC column (6.2 mm x 25 cm Zorbax-Sil) using 10% of iPrOH/hexane mixture as an eluent. 1-oxo-previtamin 14a

[UV:λ_max (Et₂O) 233.5, 297 nm] was collected at 26 ml.

Compound 14a was then reduced with LiALH₄ in anhydrous ether (-23°C, 40 min) . Excess of reagent was decomposed with water, anhydrous MgSO₄ was added and inorganic material was filtered off. After removal of the solvent, the organic residue was dissolved in EtOH and refluxed for 2.5 hours under argon atmosphere. Products were separated by HPLC (9.4 mm x 25 cm Zorbax-Sil column) using 15% iPrOH/hexane mixture. Pure 1β,25-dihydroxyvitamin D₂11a was collected at 55 ml and 1α,25-dihydroxyvitamin D₂10a at 63 ml (3:1 ratio of 11a and 10a).

The analogous reaction sequence, performed with 1α,25-dihydroxy-24-epivitamin D₂ (10b) resulted in formation of 1β,25-dihydroxy-24-epivitamin D₂ (11b) .

The following Example and its accompanying schematic, in each of which like compounds are designated by like numbers, illustrate a method for preparing the starting materials for the process of the present invention. Example 4

The C-22 aldehyde (1) is obtained by degradation of ergosterol acetate (in which the ring B diene system has been protected by Diels-Alder addition of 4-phenyl-1,2,4- triazoline-3,5-dione) according to the procedure of Barton et al. J. Chem. Sec. (c) 1968 (1971) . The i-ether aldehyde (4) is obtained from stigmasterol by the method of U.S. Patent

2,623,052.

Synthesis of the Side Chain Fragment (Sulfone A)

To a stirred solution of 4-hydroxy-3-methylbutan-2-one (12.75 g; 0.125 mol) in pyridine (100 ml) is added p-toluenesulfonyl chloride (p-TsCl) (33.25 g, 0.175 mol) in portions, and after standing for 14 hour at room temperature, the reaction mixture is poured into water and extracted with CH₂Cl₂. The extract is washed several times with aqueous CuSO₄ solution and water and then dried over anhydrous sodium sulfate. Removal of solvent under reduced pressure gives the crude tosylate which is used directly for the next reaction.

Thiophenol (14 g) dissolved in DMF (100 ml) is treated with t-BuOK (14 g) . To this reagent, the tosylate is added and after 12 hour at room temperature, the reaction mixture is poured into water and extracted with CH₂Cl₂. The extract is washed with aqueous Na₂CO₃ solution and water, then dried. Evaporation of solvent gives an oily residue which is purified by silica gel column chrcmatography. Pure phenyl sulfide is eluted with benzene (yield 15 g) .

To this phenyl sulfide derivative (15 g) , in benzene (100 ml) , ethylene glycol (6 g) and p-TsOH (20 mg) is added and the reaction mixture is heated under a Dean-Stark trap for 3 hour. After cooling, it is extracted with Na₂CO₃ solution and water, then dried and the solvent is evaporated. The product, the desired ketal, is chromatographically homogenous and can be used in the next step without further purification.

Crude ketal in dichlorσmethane (250 ml) solution is treated with m-chloroperbenzoic acid (m-CPBA) (80-85%, 27 g, added in portions) while maintaining the temperature of the reaction mixture below 30°C. After the addition of reagent, the reaction is allowed to stand at room temperature with occasional shaking. When the reaction reaches completion (about 1.5 hour) , the aromatic acids are removed by extraction with aqueous NH₃, and the organic layer is washed with water and dried. Evaporation of solvent gives the oily sulfone

(sulfone A) in essentially quantitative yield (19 g) . The product is substantially pure (homogenous by TLC) and can be used without any further purification; ¹H-NMR; δ; 1.18 (d, J = 7 Hz, 3H, CH₃-CH-), 1.19 (s, 3H, CH₃-C-) , 3.84 (m, 4H, ketal-H), 7.3-7.6 and 7.6-7.9 (m, 3H + 2H, aromatic protons);

KBr

IR, λ_max: 1305,1147,1082 cm^-1; mass spectrum, m/z (rel. intensity) : 255 (M⁺-Me, 21) , 184 (66) , 87 (92) , 43 (100) .

Coupling of Sulfcne A to Aldehyde (1) : Hydroxysulfone

(2) and Olefin (3).

Grignard reagent is prepared from Mg (535 mg; 22.22 mmol) and ethyl bromide in ether (10 ml) , and the vigorously stirred solution is treated with sulfone A (6 g; 2.22 mmol) in benzene (6 ml) . The precipitate formed is ground with a spatula, stirring is continued, and after 15 min the aldehyde (1) (2.0 g) is added in benzene (10 ml) . The reaction mixture is stirred at room temperature for 24 hour, then poured into aqueous (NH₄)₂SO₄ solution and extracted with benzene. The organic layer, after washing with water, drying and evaporation gives an oily residue which is chrcmatographed on silica gel. In the benzene-ether fractions (8:2) , excess sulfone is recovered (4.5 g); elution with benzene-ether (3:1) affords unreacted aldehyde (1) (1.0 g) ; the reaction products (2) are eluted with ethyl acetate.

The crude mixture of steroidal α-hydroxysulfones (2) is dissolved in methanol (200 ml) saturated with Na₂HPO₄. Sodium amalgam (5.65%, 15 g) is added and the reaction mixture is stirred at 4°C for 15 hour.

After completion of the Na/Hg reduction, mercury is removed by filtration, and irethanol by evaporation under reduced pressure, water is added and the organic material is extracted with benzene. After drying and evaporation of solvent, the oily residue is chromatographed on a silica gel column. Elution with benzene-ether (1:4) gives compound (3) a colorless foam; ¹Η-NMR, δ: 0.80 (s, 18-H) , 0.97 (s, 19-H), 1.22 (s, 26-H), 3.93 (m, 4H, ketal-H) , 4.44 (m, 1H, 3 -H) , 5.25-5.45 (m, 2H, 22-H and 23-H), 6.23 and 6.39 (doublets, J = 8 Hz, 2 x 1H, 7-H and 6-H), 7.25-7.45 (m, 5H, -C₆H₅) ; IR, 3603 (0-H) , 1749, 1692 (C=O) , 1406,1038 cm^-1;

mass spectrum, m/z: 440 (M⁺ - triazoline, 24) , 87 (100) .

(To increase yield, unreacted aldehyde (1) , as recovered above, can be recycled through the sulfone addition, and the resulting α-hydroxy sulfones (2) are then, as above, treated with sodium amalgam in buffered rrethanol to provide additional olefin (3) . The above reactions are preferably conducted under an inert atmosphere, such as argon.)

Coupling of Sulfone A to Aldehyde (4) : Hydroxysulfone (5) and Olefin (6) .

Grignard reagent is prepared from Mg (75 mg, 3.1 mmol) and ethyl bromide in ether (10 ml) . To the stirred solution of ethyl magnesium bromide, sulfone A (891 mg; 3.3 mmol) in benzene (5 ml) is added. After stirring the resulting suspension at room temperature for 15 min, a solution of aldehyde (4) (290 mg) in benzene (5 ml) is added. The reaction is continued for 2.5 h, then quenched with saturated

(NH₄)₂SO₄ solution (5 ml) and diluted with ether. The separated organic layer is washed with water, dried, and evaporated. The oily residue containing (5) is treated with acetic anhydride (2 ml) and pyridine (2 ml) . The reaction mixture is allowed to stand for 24 hours, poured into water and extracted with benzene. The benzene extract is washed with an aqueous solution of CuSO₄, water, dried, and evaporated. The crude product [the acetate of (5) ] is dissolved in methanol saturated with Na₂HPO₄ and sodium amalgam (5.65%, 8 g) is added. The reaction mixture is stirred at 4°C for 16 hours. After the reaction, mercury is removed by filtration, methanol is evaporated, and water and benzene are added to dissolve the residue. The benzene layer is dried and evaporated. The oily residue is chromatographed over silica gel. Elution with benzene-ether mixture (93:7) affords compound (6) (206 mg; 54%), ¹H-NMR, δ: 0.74 (s, 18-H) ,

1.04 (s, 19-H), 1.25 (s, 26-H), 2.78 (m, 1H, 6 -H) , 3.34 (s,

3H, -OCH₃), 3.97 (m, 4H, ketal-H), 5.25-5.45 (m, 2H, 22-H and

23-H) , IR, 3470 (O-H) , 1095 cm^-1; mass spectrum, m/z

(rel. intensity): 456 (M⁺, 1), 441 (M⁺-Me, 45), 87 (100). It should be noted that the acetylation step described above is not essential and may be emitted if desired; i.e. the hydroxysulfone (5) may be submitted directly to

Na/Hg-reduction, as in Example 3. The above reactions are preferably conducted under an inert atmosphere, e.g. argon.

Removal of PTAD-protecting Group: 5,7-Diene (7).

A mixture of the compound (3) (1 g) and lithium aluminum hydride (1.8 g) in THF (120 ml) is heated under reflux for 10 hours. After cooling, excess reagent is destroyed with a few drops of water, and the mixture is dried over anhydrous.MgSO₄, filtered, and solvent is evaporated to give colorless crystalline material. Crude diene 7 is repeatedly crystallized from ethanol; first and second crops combined give 415 mg of (7) . The mother liquor is chromatographed on silica gel column, to give with benzene-ether (7:3) , an additional 120 mg of (7) , total yield 535 mg (79%) ; m.p.

132-134°C (from ethanol) , ¹H-NMR, δ: 0.63 (s, 18-H), 0.95 (s,

19-H), 1.23 (s, 26-H), 3.63 (m, 1H, 3 -H) , 3.95 (m, 4H, ketal-H), 5.20-5.50 (m, 3H, 22-H, 23-H and 7-H), 5.57 (m, 1H,

6-H) ; IR, 3430 (O-H) , 1063, 1038 cm^-1; mass spectrum,

m/z (rel. int.) : 440 (M , 50) , 407 (M⁺ -H₂O-Me, 11) , 87 (100) ; UV, 282 nm (ε = 11,000) .

Irradiation of Compound (7) : Previtamin Analog (8) .

A solution of diene (7) (50 mg) in 150 ml of benzene-ether (1:4) is cooled on ice and deoxygenated with argon for 20 min. The reaction mixture is irradiated under argon atmosphere for 18 min with a mercury arc lamp (Hanovia SA-1) fitted with a Vycor filter. The solvent is evaporated and the residue is chromatographed on HPLC (6.2 irm x 25 cm microparticulate silica gel, 4 ml/min, 1400 psi) and eluted with 2% 2-propanol in hexane to yield 22 mg (44%) of previtamin (8) ; ¹H-NMR; δ : 0.73 (S, 18-H), 1.24 (s, 26-H), 1.64 (s, 19-H), 3.96 (m, 5H, ketal-H and 3 -H) , 5.35 (m, 2H, 22-H and 23-H), 5.50 (m, 1H, 9-H) , 5.69 and 5.94 (doublets, J - 11.5 Hz, 2 x 1H, 6-H and 7-H) ; UV, 263 nm

(ε = 8,900) .

Isomerization of (8) to the Vitamin-Analog (9) .

Previtamin 8 (22 mg) is dissolved in ethanol (40 ml) and heated under reflux for 150 min (argon atmosphere) . The product is purified by HPLC to yield 18 mg (82%) of the pure vitamin (9) ; ¹H-NMR, δ : 0.75 (s, 18-H), 1.24 (s, 26-H), 3.94 (m, 5H, ketal-H and 3 -H) , 4.81 and 5.04 (2 narrow m, 2 x 1H, 19 (Z)- and 19(E)-H), 5.33 (m, 2H, 22-H and 23-H), 6.03 (d, J = 11 Hz, 1H, 7-H), 6.22 (d, J = 11 Hz, 1H, 6-H); mass spectrum, m/z (rel. int.): 440. (M⁺, 17), 87 (100) , UV, 265 nm (ε = 17,000).

Hydrolysis of the ketal: Keto-Vitamin D₂-Analog (10) .

To the solution of compound (9) (18 mg) in ethanol (35 ml), p-toluenesulfonic acid (7.5 mg) in water (1 ml) is added and the reaction mixture is heated under reflux for 90 min

(the reaction course is monitored by HPLC) . The solvent is evaporated, the residue is dissolved in benzene and extracted with water. The benzene solution is dried (anhydrous MgSO₄) , and evaporated to yield product (10) (16 mg; 99%) . ¹H-NMR, δ:

0.57 (s, 18-H) , 1.04 (d, J = 7 Hz, 21-H) , 1.13 (d, J = 7 Hz,

28-H), 2.12 (s, 3H, 26-H), 3.10 (m, 1H, 24-H) , 3.96 (m, 1H, 3

-H) , 4.82 and 5.05 (2 narrow m, 2 x 1 H, 19 (Z)- and 19(E)-H) ,

5.2-5.5 (m, 2H, 22-H and 23-H), 6.03 (d, J = 11.5 Hz, 1H,

7-H), 6.22 (d, J = 11.5 Hz, 1H, 6-H), IR, 3596 (O-H),

1709 cm^-1 (C=O); mass spectrum, m/z (rel. int.): 396 (M⁺, 41), 363 (M⁺-H₂O-Me, 13) , 271 (M⁺-side chain, 16) , 253 (m⁺-side chain-H₂O, 23) , 136 (100) , 118 (95) ; UV, 265 nm (ε =

17,900). Reaction of Ketone (10) with Methylmagnesium Iodide: 25-OH-D₂, (11a) , and its Epimer (11b) .

Grignard reagent is prepared from magnesium (240 mg) and methyl iodide in anhydrous ether (20 ml) . To one-tenth of this solution (2 ml; 0.5 M solution of CH₃Mgl) ketone (10) (16 mg; 0.04 mmol) in ether (2 ml) is added. The reaction mixture is stirred at room temperature for 2 hours under an inert atmosphere, then quenched with aqueous solution of NH₄Cl, diluted with benzene and washed with water. The organic layer is separated, dried and evaporated. The crude product is first purified by silica gel column chromatography (elution with 20% ether in benzene) and the mixture of (11a) and (11b)

(16 mg; 96%) thereby obtained is then repeatedly chromatographed on HPLC column using 2% 2-propanol in hexane as an eluent to separate the 24-stereoisomers, 24-epi-25-OH-D₂

(11b) and 25-OH-D₂ (11a) . Chromatography and rechrcmatography of each stereoisomer yields 4 mg of (11b) (collected at 68 ml) , 4 mg of (11a) (collected at 74 ml) and 7 mg of the mixture of both epimers. Treatment of 2 mg of the epimer mixture with excess acetic anhydride in pyridine solution at room temperature overnight followed by standard work-up yields the corresponding 3-O-acetates.

25-OH-D₂ (11a) : + 56.8° (C = 0.2 in EtOH) ; ¹H-NMR,

δ: 0.57 (s, 18-H), 1.00 (d, J = 7 Hz, 28-H) , 1.04 (d, J = 7

Hz, 21-H), 1.15 and 1.17 (2 singlets, 26-H and 27-H), 3.95 (m,

1H, 3 -H) , 4.82 and 5.05 (2 narrow m, 2 x 1H, 19(Z)- and

19(E)-H), 5.23-5.43 (m, 2H, 22-H and 23-H), 6.05 and 6.22 (2 doublets, J = 11 Hz, 2 x 1H, 7-H and 6-H) ; IR, 3401

(O-H), 1645, 1631 (C=C) , 971 cm-1 (trans C=C) ; mass spectrum, m/z (rel. int.): 412 (M⁺, 63), 394 (M⁺-H₂O, 10), 379 (M⁺-H₂O-Me, 23) , 271 (M⁺-side chain, 37) , 253 (M⁺-side chain-H₂O, 43) , 136 (100) , 118 (86) , 59 (99) , UV, 265 nm (ε = 17,950).

24-epi-25-OH-D₂ (11b): 50.7° (C = 0.2 in EtOH) ,

1H-NMR, δ : 0.57 (s, 18-H), 0.99 (d, J = 7 Hz, 28-H), 1.03 (d, J = 7 Hz, 21-H), 1.14 and 1.16 (2 singlets, 26-H and 27-H) , 3.94 (m, IH, 3 -H) , 4.82 and 5.03 (2 narrow m, 2 x 1H, 19(Z)- and 19(E)-H), 5.20-5.40 (m, 2H, 22-H and 23-H), 6.04 and 6.22

(2 doublets, J = 11 Hz, 2 x 1H, 7-H and 6-H) , IR, 3401

(OH) , 1643, 1630 (C=C) , 971 cm (trans C=C) ; mass spectrum, m/z (rel. int.): 412 (M⁺, 62) 394 (M⁺-H₂O; 12), 379 (M⁺-H₂O-Me, 31) , 271 (M⁺-side chain, 44) , 253 (M⁺-side chain-H₂O, 55) , 136 (100) , 118 (67) , 59 (38) ; UV, 265

nm (ε = 17,300) .

It should be noted that from pure provitamin (7) further synthesis (i.e. the irradiation, iscmerization, deketalization and Grignard reaction steps) may be accomplished without chromatographic purification of any intermediate. Careful column chromatography on silica gel before the final separation on HPLC removes all by-products.

Scheme for Preparing Starting Compounds

Claims

1. A compound selected from the group consisting of

wherein R₁, R₂ and R₃ are each selected from the group consisting of hydrogen and acyl, and where the methyl group at carbon

24 may have the R or S configuration.

2. A compound according to Claim 1 having the 24R configuration.

3. A compound according to Claim 1, having the 24S configuration.

4. 1β,25-dihydroxyvitamin D₂.

5. A pharmaceutical preparation comprising the compound of Claim 4 and a pharmaceutically acceptable excipient.

6. 1β,25-dihydroxy-24-epivitamin D₂.

7. A pharmaceutical preparation comprising the compound of Claim 6 and a pharmaceutically acceptable excipient.

8. 1β, 25-dihydroxy-5,6-trans-vitamin D₂.

9. A pharmaceutical preparation comprising the compound of Claim 8 and a pharmaceutically acceptable excipient.

10. 1β,25-dihydroxy-5,6-trans-24-epivitamin D₂.

11. A pharmaceutical preparation comprising the compound of Claim 10 and a pharmaceutically acceptable excipient.

12. A pharmaceutical preparation comprising an admixture of the ccmpounds of Claim 4 and Claim 6, together with a pharmaceutically acceptable excipient.

13. A pharmaceutical preparation comprising an admixture of the compounds of Claim 8 and Claim 10 and a pharmaceutically acceptable excipient.

14. A compound having the formula:

where the methyl group at carbon 24 may have the R or S configuration.