US7709629B2 - siRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) - Google Patents
siRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) Download PDFInfo
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- US7709629B2 US7709629B2 US11/978,398 US97839807A US7709629B2 US 7709629 B2 US7709629 B2 US 7709629B2 US 97839807 A US97839807 A US 97839807A US 7709629 B2 US7709629 B2 US 7709629B2
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- C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
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Definitions
- RNAi exhibits sequence specificity. Kisielow, M. et al., (2002) Isoform-specific knockdown and expression of adaptor protein ShcA using small interfering RNA, J. Biochem. 363: 1-5. Thus, the RNAi machinery can specifically knock down one type of transcript, while not affecting closely related mRNA. These properties make siRNA a potentially valuable tool for inhibiting gene expression and studying gene function and drug target validation. Moreover, siRNAs are potentially useful as therapeutic agents against: (1) diseases that are caused by over-expression or misexpression of genes; and (2) diseases brought about by expression of genes that contain mutations.
- the first sense region comprises a sequence that is identical to at least 18 bases of a sequence selected from the group consisting of SEQ ID NO. 438 to SEQ ID NO. 568
- said second sense region comprises a sequence that is identical to at least 18 bases of a sequence selected from the group consisting of SEQ ID NO. 438 to SEQ ID NO. 568
- the duplex of said first siRNA is 19-30 base pairs
- said first sense region comprises a sequence that is at least 90% similar to a sequence selected from the group consisting of SEQ ID NO. 438 to SEQ ID NO. 568
- said duplex of said second siRNA is 19-30 base pairs and comprises a sequence that is at least 90% similar to a sequence selected from the group consisting of SEQ ID NO. 438 to SEQ ID NO. 568.
- FIG. 4 is a representation of a statistical analysis that revealed correlations between silencing and five sequence-related properties of siRNA: (A) an A at position 19 of the sense strand, (B) an A at position 3 of the sense strand, (C) a U at position 10 of the sense strand, (D) a base other than G at position 13 of the sense strand, and (E) a base other than C at position 19 of the sense strand. All variables were correlated with siRNA silencing of firefly luciferase and human cyclophilin. siRNAs satisfying the criterion are grouped on the left (Selected) while those that do not, are grouped on the right (Eliminated). Y-axis is “% Silencing of Control.” Each position on the X-axis represents a unique siRNA.
- Modified nucleotides also include those nucleotides that are modified with respect to the sugar moiety, as well as nucleotides having sugars or analogs thereof that are not ribosyl.
- the sugar moieties may be, or be based on, mannoses, arabinoses, glucopyranoses, galactopyranoses, 4′-thioribose, and other sugars, heterocycles, or carbocycles.
- SMARTSCORETM or “siRNA rank” refers to a number determined by applying any of the formulas to a given siRNA sequence.
- SMART-selected or “rationally selected” or “rational selection” refers to siRNA that have been selected on the basis of their SMARTSCORESTM, or siRNA ranking.
- U 4 1 if U is the base at position 4 on the sense strand, otherwise its value is 0;
- RISC is a complex of approximately twelve proteins; Dicer is one, but not the only, helicase within this complex. Accordingly, although the GC parameters are believed to relate to activity with Dicer, they are also important for activity with other RISC proteins.
- kits may be applied either in vivo or in vitro.
- the siRNA of the pools or kits is applied to a cell through transfection, employing standard transfection protocols. These methods are well known to persons skilled in the art and include the use of lipid-based carriers, electroporation, cationic carriers, and microinjection. Further, one could apply the present invention by synthesizing equivalent DNA sequences (either as two separate, complementary strands, or as hairpin molecules) instead of siRNA sequences and introducing them into cells through vectors. Once in the cells, the cloned DNA could be transcribed, thereby forcing the cells to generate the siRNA.
- siRNA that exhibit hyperfunctional potency i.e., those that induce 80% silencing or greater at, e.g., picomolar concentrations
- siRNA having high (> ⁇ 10) SMARTSCORESTM, or siRNA rankings and greater than 80% silencing are examined.
- siRNA are introduced into a test cell line and the levels of RNAi are measured over an extended period of time (e.g., 24-168 hrs).
- siRNAs that exhibit strong RNA interference patterns i.e., >80% interference
- periods of time greater than, e.g., 120 hours are thus identified.
Abstract
Description
(−14)*G13−13*A1−12*U7−11*U2−10*A11−10*U4−10*C3−10*C5−10*C6−9*A10−9*U9−9*C18−8*G10−7*U1−7*U16−7*C17−7*C19+7*U17+8*A2+8*A4+8*A5+8*C4+9*G8+10*A7+10*U18+11*A19+11*C9+15*G1+18*A3+19*U10−Tm−3*(GCtotal)−6*(GC15-19)−30*X; or Formula VIII
(−8)*A1+(−1)*A2+(12)*A3+(7)*A4+(18)*A5+(12)*A6+(19)*A7+(6)*A8+(−4)*A9+(−5)*A10+(−2)*A11+(−5)*A12+(17)*A13+(−3)*A14+(4)*A15+(2)*A16+(8)*A17+(11)*A18+(30)*A19+(−13)*U1+(−10)*U2+(2)*U3+(−2)*U4+(−5)*U5+(5)*U6+(−2)*U7+(−10)*U8+(−5)*U9+(15)*U10+(−1)*U11+(0)*U12+(10)*U13+(−9)*U14+(−13)*U15+(−10)*U16+(3)*U17+(9)*U18+(9)*U19+(7)*C1+(3)*C2+(−21)*C3+(5)*C4+(−9)*C5+(−20)*C6+(−18)*C7+(−5)*C8+(5)*C9+(1)*C10+(2)*C11+(−5)*C12+(−3)*C13+(−6)*C14+(−2)*C15+(−5)*C16+(−3)*C17+(−12)*C18+(−18)*C19+(14)*G1+(8)*G2+(7)*G3+(−10)*G4+(−4)*G5+(2)*G6+(1)*G7+(9)*G8+(5)*G9+(−11)*G10+(1)*G11+(9)*G12+(−24)*G13+(18)*G14+(11)*G15+(13)*G16+(−7)*G17+(−9)*G18+(−22)*G19+6*(number of A+U in position 15-19)−3*(number of G+C in whole siRNA), Formula X
wherein position numbering begins at the 5′-most position of a sense strand, and
A1=1 if A is the base at
A2=1 if A is the base at
A3=1 if A is the base at
A4=1 if A is the base at
A5=1 if A is the base at
A6=1 if A is the base at
A7=1 if A is the base at
A10=1 if A is the base at
A11=1 if A is the base at
A13=1 if A is the base at
A19=1 if A is the base at
C3=1 if C is the base at
C4=1 if C is the base at
C5=1 if C is the base at
C6=1 if C is the base at
C7=1 if C is the base at
C9=1 if C is the base at
C17=1 if C is the base at
C18=1 if C is the base at
C19=1 if C is the base at
G1=1 if G is the base at
G2=1 if G is the base at
G8=1 if G is the base at
G10=1 if G is the base at
G13=1 if G is the base at
G19=1 if G is the base at
U1=1 if U is the base at
U2=1 if U is the base at
U3=1 if U is the base at
U4=1 if U is the base at
U7=1 if U is the base at
U9=1 if U is the base at
U10=1 if U is the base at
U15=1 if U is the base at
U16=1 if U is the base at
U17=1 if U is the base at
U18=1 if U is the base at
GC15-19=the number of G and C bases within positions 15-19 of the sense strand, or within positions 15-18 if the sense strand is only 18 base pairs in length;
GCtotal=the number of G and C bases in the sense strand;
Tm=100 if the siRNA oligo has the internal repeat longer then 4 base pairs, otherwise its value is 0; and
X=the number of times that the same nucleotide repeats four or more times in a row.
(−14)*G13−13*A1−12*U7−11*U2−10*A11−10*U4−10*C3−10*C5−10*C6−9*A10−9*U9−9*C18−8*G10−7*U1−7*U16−7*C17−7*C19+7*U17+8*A2+8*A4+8*A5+8*C4+9*G8+10*A7+10*U18+11*A19+11*C9+15*G1+18*A3+19*U10−Tm−3*(GCtotal)−6*(GC15-19)−30*X; or Formula VIII
(−8)*A1+(−1)*A2+(12)*A3+(7)*A4+(18)*A5+(12)*A6+(19)*A7+(6)*A8+(−4)*A9+(−5)*A10+(−2)*A11+(−5)*A12+(17)*A13+(−3)*A14+(4)*A15+(2)*A16+(8)*A17+(11)*A18+(30)*A19+(−13)*U1+(−10)*U2+(2)*U3+(−2)*U4+(−5)*U5+(5)*U6+(−2)*U7+(−10)*U8+(−5)*U9+(15)*U10+(−1)*U11+(0)*U12+(10)*U13+(−9)*U14+(−13)*U15+(−10)*U16+(3)*U17+(9)*U18+(9)*U19+(7)*C1+(3)*C2+(−21)*C3+(5)*C4+(−9)*C5+(−20)*C6+(−18)*C7+(−5)*C8+(5)*C9+(1)*C10+(2)*C11+(−5)*C12+(−3)*C13+(−6)*C14+(−2)*C15+(−5)*C16+(−3)*C17+(−12)*C18+(−18)*C19+(14)*G1+(8)*G2+(7)*G3+(−10)*G4+(−4)*G5+(2)*G6+(1)*G7+(9)*G8+(5)*G9+(−11)*G10+(1)*G11+(9)*G12+(−24)*G13+(18)*G14+(11)*G15+(13)*G16+(−7)*G17+(−9)*G18+(−22)*G19+6*(number of A+U in position 15-19)−3*(number of G+C in whole siRNA), Formula X
SEQ. ID NO. 0001. | NNANANNNNUCNAANNNNA | ||
SEQ. ID NO. 0002. | NNANANNNNUGNAANNNNA | ||
SEQ. ID NO. 0003. | NNANANNNNUUNAANNNNA | ||
SEQ. ID NO. 0004. | NNANANNNNUCNCANNNNA | ||
SEQ. ID NO. 0005. | NNANANNNNUGNCANNNNA | ||
SEQ. ID NO. 0006. | NNANANNNNUUNCANNNNA | ||
SEQ. ID NO. 0007. | NNANANNNNUCNUANNNNA | ||
SEQ. ID NO. 0008. | NNANANNNNUGNUANNNNA | ||
SEQ. ID NO. 0009. | NNANANNNNUUNUANNNNA | ||
SEQ. ID NO. 0010. | NNANCNNNNUCNAANNNNA | ||
SEQ. ID NO. 0011. | NNANCNNNNUGNAANNNNA | ||
SEQ. ID NO. 0012. | NNANCNNNNUUNAANNNNA | ||
SEQ. ID NO. 0013. | NNANCNNNNUCNCANNNNA | ||
SEQ. ID NO. 0014. | NNANCNNNNUGNCANNNNA | ||
SEQ. ID NO. 0015. | NNANCNNNNUUNCANNNNA | ||
SEQ. ID NO. 0016. | NNANCNNNNUCNUANNNNA | ||
SEQ. ID NO. 0017. | NNANCNNNNUGNUANNNNA | ||
SEQ. ID NO. 0018. | NNANCNNNNUUNUANNNNA | ||
SEQ. ID NO. 0019. | NNANGNNNNUCNAANNNNA | ||
SEQ. ID NO. 0020. | NNANGNNNNUGNAANNNNA | ||
SEQ. ID NO. 0021. | NNANGNNNNUUNAANNNNA | ||
SEQ. ID NO. 0022. | NNANGNNNNUCNCANNNNA | ||
SEQ. ID NO. 0023. | NNANGNNNNUGNCANNNNA | ||
SEQ. ID NO. 0024. | NNANGNNNNUUNCANNNNA | ||
SEQ. ID NO. 0025. | NNANGNNNNUCNUANNNNA | ||
SEQ. ID NO. 0026. | NNANGNNNNUGNUANNNNA | ||
SEQ. ID NO. 0027. | NNANGNNNNNUNUANNNNA |
Relative functionality of siRNA=−(GC/3)+(AU 15-19)−(Tm 20° C.)*3−(G 13)*3−(C 19)+(A 19)*2+(A 3)+(U 10)+(A 14)−(U 5)−(A 11) Formula I
Relative functionality of siRNA=−(GC/3)−(AU 15-19)*3−(G 13)*3−(C 19)+(A 19)*2+(A 3) Formula II
Relative functionality of siRNA=−(GC/3)+(AU 15-19)−(Tm 20° C.)*3 Formula III
Relative functionality of siRNA=−GC/2+(AU 15-19)/2−(Tm 20° C.)*2−(G 13)*3−(C 19)+(A 19)*2+(A 3)+(U 10)+(A 14)−(U 5)−(A 11) Formula IV
Relative functionality of siRNA=−(G 13)*3−(C 19)+(A 19)*2+(A 3)+(U 10)+(A 14)−(U 5)−(A 11) Formula V
Relative functionality of siRNA=−(G 13)*3−(C 19)+(A 19)*2+(A 3) Formula VI
Relative functionality of siRNA=−(GC/2)+(AU 15-19)/2−(Tm 20° C.)*1−(G 13)*3−(C 19)+(A 19)*3+(A 3)*3+(U 10)/2+(A 14)/2−(U 5)/2−(A 11)/2 Formula VII
TABLE I |
FUNCTIONAL PROBABILITY |
OLIGOS | POOLS |
CRITERIA | >95% | >80% | <70% | >95% | >80% | <70% |
CURRENT | 33.0 | 50.0 | 23.0 | 79.5 | 97.3 | 0.3 |
NEW | 50.0 | 88.5 | 8.0 | 93.8 | 99.98 | 0.005 |
(GC) | 28.0 | 58.9 | 36.0 | 72.8 | 97.1 | 1.6 |
TABLE II |
FUNCTIONAL PROBABILITY |
OLIGOS | POOLS |
NON- | NON- | ||||||
FUNCTIONAL | AVERAGE | FUNCTIONAL | FUNCTIONAL | AVERAGE | FUNCTIONAL | ||
RANDOM | 20 | 40 | 50 | 67 | 97 | 3 |
|
52 | 99 | 0.1 | 97 | 93 | 0.0040 |
|
89 | 99 | 0.1 | 99 | 99 | 0.0000 |
(−14)*G13−13*A1−12*U7−11*U2−10*A11−10*U4−10*C3−10*C5−10*C6−9*A10−9*U9−9*C18−8*G10−7*U1−7*U16−7*C17−7*C19+7*U17+8*A2+8*A4+8*A5+8*C4+9*G8+10*A7+10*U18+11*A19+11*C9+15*G1+18*A3+19*U10−Tm−3*(GCtotal)−6*(GC15-19)−30*X; and Formula VIII
(14.1)*A3+(14.9)*A6+(17.6)*A13+(24.7)*A19+(14.2)*U10+(10.5)*C9+(23.9)*G1+(16.3)*G2+(−12.3)*A11+(−19.3)*U1+(−12.1)*U2+(−11)*U3+(−15.2)*U15+(−11.3)*U16+(−11.8)*C3+(−17.4)*C6+(−10.5)*C7+(−13.7)*G13+(−25.9)*G19−Tm−3*(GCtotal)−6*(GC15-19)−30*X; and Formula IX
(−8)*A1+(−1)*A2+(12)*A3+(7)*A4+(18)*A5+(12)*A6+(19)*A7+(6)*A8+(−4)*A9+(−5)*A10+(−2)*A11+(−5)*A12+(17)*A13+(−3)*A14+(4)*A15+(2)*A16+(8)*A17+(11)*A18+(30)*A19+(−13)*U1+(−10)*U2+(2)*U3+(−2)*U4+(−5)*U5+(5)*U6+(−2)*U7+(−10)*U8+(−5)*U9+(15)*U10+(−1)*U11+(0)*U12+(10)*U13+(−9)*U14+(−13)*U15+(−10)*U16+(3)*U17+(9)*U18+(9)*U19+(7)*C1+(3)*C2+(−21)*C3+(5)*C4+(−9)*C5+(−20)*C6+(−18)*C7+(−5)*C8+(5)*C9+(1)*C10+(2)*C11+(−5)*C12+(−3)*C13+(−6)*C14+(−2)*C15+(−5)*C16+(−3)*C17+(−12)*C18+(−18)*C19+(14)*G1+(8)*G2+(7)*G3+(−10)*G4+(−4)*G5+(2)*G6+(1)*G7+(9)*G8+(5)*G9+(−11)*G10+(1)*G11+(9)*G12+(−24)*G13+(18)*G14+(11)*G15+(13)*G16+(−7)*G17+(−9)*G18+(−22)*G19+6*(number of A+U in position 15-19)−3*(number of G+C in whole siRNA). Formula X
Human cyclophilin: 193-390, M60857 | ||
SEQ. ID NO. 29: | ||
gttccaaaaa cagtggataa ttttgtggcc ttagctacag | ||
gagagaaagg atttggctac aaaaacagca aattccatcg | ||
tgtaatcaag gacttcatga tccagggcgg agacttcacc | ||
aggggagatg gcacaggagg aaagagcatc tacggtgagc | ||
gcttccccga tgagaacttc aaactgaagc actacgggcc | ||
tggctggg |
Firefly luciferase: 1434-1631, U47298 (pGL3, | ||
Promega) | ||
SEQ. ID NO. 30: | ||
tgaacttccc gccgccgttg ttgttttgga gcacggaaag | ||
acgatgacgg aaaaagagat cgtggattac gtcgccagtc | ||
aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt | ||
tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac | ||
gcaagaaaaa tcagagagat cctcataaag gccaagaagg |
DBI, NM_020548 (202-310) (every position) | ||
SEQ. ID NO. 0031: | ||
acgggcaagg ccaagtggga tgcctggaat gagctgaaag | ||
ggacttccaa ggaagatgcc atgaaagctt acatcaacaa | ||
agtagaagag ctaaagaaaa aatacggg |
TABLE IV | ||
IMPROVEMENT | ||
PERCENT | OVER | |
CRITERION | FUNCTIONAL | RANDOM (%) |
I. | 30%-52% G/C Content | <F50 | 16.4 | −3.6 |
≧F50 | 83.6 | 3.6 | ||
≧F80 | 60.4 | 4.3 | ||
≧F95 | 23.9 | 2.2 | ||
II. | At least 3 A/U | <F50 | 18.2 | −1.8 |
bases at positions | ≧F50 | 81.8 | 1.8 | |
15-19 of the sense | ≧F80 | 59.7 | 3.6 | |
strand | ≧F95 | 24.0 | 2.3 | |
III. | Absence of internal | <F50 | 16.7 | −3.3 |
repeats, as measured | ≧F50 | 83.3 | 3.3 | |
by Tm of secondary | ≧F80 | 61.1 | 5.0 | |
structure ≦20° C. | ≧F95 | 24.6 | 2.9 | |
IV. | An A base at | <F50 | 11.8 | −8.2 |
| ≧F50 | 88.2 | 8.2 | |
of the sense strand | ≧F80 | 75.0 | 18.9 | |
≧F95 | 29.4 | 7.7 | ||
V. | An A base at | <F50 | 17.2 | −2.8 |
| ≧F50 | 82.8 | 2.8 | |
the sense strand | ≧F80 | 62.5 | 6.4 | |
≧F95 | 34.4 | 12.7 | ||
VI. | A U base at | <F50 | 13.9 | −6.1 |
| ≧F50 | 86.1 | 6.1 | |
the sense strand | ≧F80 | 69.4 | 13.3 | |
≧F95 | 41.7 | 20 | ||
VII. | A base other than | <F50 | 18.8 | −1.2 |
C at | ≧F50 | 81.2 | 1.2 | |
of the sense strand | ≧F80 | 59.7 | 3.6 | |
≧F95 | 24.2 | 2.5 | ||
VIII. | A base other than | <F50 | 15.2 | −4.8 |
G at | ≧F50 | 84.8 | 4.8 | |
of the sense strand | ≧F80 | 61.4 | 5.3 | |
≧F95 | 26.5 | 4.8 | ||
The siRNA Selection Algorithm
(160+unadjusted score)/2.25
(228+unadjusted score)/3.56
| ||||
Cyclo | ||||
1 | SEQ. ID 0032 | | ||
Cyclo | ||||
2 | SEQ. ID 0033 | | ||
Cyclo | ||||
3 | SEQ. ID 0034 | | ||
Cyclo | ||||
4 | SEQ. ID 0035 | | ||
Cyclo | ||||
5 | SEQ. ID 0036 | | ||
Cyclo | ||||
6 | SEQ. ID 0037 | | ||
Cyclo | ||||
7 | SEQ. ID 0038 | | ||
Cyclo | ||||
8 | SEQ. ID 0039 | | ||
Cyclo | ||||
9 | SEQ. ID 0040 | | ||
Cyclo | ||||
10 | SEQ. ID 0041 | | ||
Cyclo | ||||
11 | SEQ. ID 0042 | | ||
Cyclo | ||||
12 | SEQ. ID 0043 | | ||
Cyclo | ||||
13 | SEQ. ID 0044 | | ||
Cyclo | ||||
14 | SEQ. ID 0045 | | ||
Cyclo | ||||
15 | SEQ. ID 0046 | | ||
Cyclo | ||||
16 | SEQ. ID | UUAGCUACAGGAGAGAAAG | ||
Cyclo | ||||
17 | SEQ. ID 0048 | | ||
Cyclo | ||||
18 | SEQ. ID 0049 | | ||
Cyclo | ||||
19 | SEQ. ID 0050 | | ||
Cyclo | ||||
20 | SEQ. ID 0051 | | ||
Cyclo | ||||
21 | SEQ. ID 0052 | | ||
Cyclo | ||||
22 | SEQ. ID 0053 | | ||
Cyclo | ||||
23 | SEQ. ID 0054 | | ||
Cyclo | ||||
24 | SEQ. ID 0055 | | ||
Cyclo | ||||
25 | SEQ. ID 0056 | | ||
Cyclo | ||||
26 | SEQ. ID 0057 | | ||
Cyclo | ||||
27 | SEQ. ID 0058 | | ||
Cyclo | ||||
28 | SEQ. ID 0059 | | ||
Cyclo | ||||
29 | SEQ. ID 0060 | | ||
Cyclo | ||||
30 | SEQ. ID 0061 | | ||
Cyclo | ||||
31 | SEQ. ID 0062 | | ||
Cyclo | ||||
32 | SEQ. ID 0063 | AAACAGCAAAUUCCAUCGU | ||
Cyclo | 33 | SEQ. ID 0064 | | |
Cyclo | ||||
34 | SEQ. ID 0065 | AGCAAAUUCCAUCGUGUAA | ||
Cyclo | 35 | SEQ. ID 0066 | | |
Cyclo | ||||
36 | SEQ. ID 0067 | | ||
Cyclo | ||||
37 | SEQ. ID 0068 | UUCCAUCGUGUAAUCAAGG | ||
Cyclo | 38 | SEQ. ID 0069 | CCAUCGUGUAAUCAAGGAC | |
Cyclo | 39 | SEQ. ID 0070 | | |
Cyclo | ||||
40 | SEQ. ID 0071 | CGUGUAAUCAAGGACUUCA | ||
Cyclo | 41 | SEQ. ID 0072 | | |
Cyclo | ||||
42 | SEQ. ID 0073 | UAAUCAAGGACUUCAUGAU | ||
Cyclo | 43 | SEQ. ID 0074 | AUCAAGGACUUCAUGAUCC | |
Cyclo | 44 | SEQ. ID 0075 | CAAGGACUUCAUGAUCCAG | |
Cyclo | 45 | SEQ. ID 0076 | AGGACUUCAUGAUCCAGGG | |
Cyclo | 46 | SEQ. ID 0077 | | |
Cyclo | ||||
47 | SEQ. ID 0078 | CUUCAUGAUCCAGGGCGGA | ||
Cyclo | 48 | SEQ. ID 0079 | UCAUGAUCCAGGGCGGAGA | |
Cyclo | 49 | SEQ. ID 0080 | | |
Cyclo | ||||
50 | SEQ. ID 0081 | GAUCCAGGGCGGAGACUUC | ||
Cyclo | 51 | SEQ. ID 0082 | | |
Cyclo | ||||
52 | SEQ. ID 0083 | CAGGGCGGAGACUUCACCA | ||
Cyclo | 53 | SEQ. ID 0084 | GGGCGGAGACUUCACCAGG | |
Cyclo | 54 | SEQ. ID 0085 | GCGGAGACUUCACCAGGGG | |
Cyclo | 55 | SEQ. ID 0086 | | |
Cyclo | ||||
56 | SEQ. ID 0087 | | ||
Cyclo | ||||
57 | SEQ. ID 0088 | | ||
Cyclo | ||||
58 | SEQ. ID 0089 | UUCACCAGGGGAGAUGGCA | ||
Cyclo | 59 | SEQ. ID 0090 | | |
Cyclo | ||||
60 | SEQ. ID 0091 | CCAGGGGAGAUGGCACAGG | ||
Cyclo | 61 | SEQ. ID 0092 | | |
Cyclo | ||||
62 | SEQ. ID 0093 | | ||
Cyclo | ||||
63 | SEQ. ID 0094 | GAGAUGGCACAGGAGGAAA | ||
Cyclo | 64 | SEQ. ID 0095 | GAUGGCACAGGAGGAAAGA | |
Cyclo | 65 | SEQ. ID 0096 | UGGCACAGGAGGAAAGAGC | |
Cyclo | 66 | SEQ. ID 0097 | GCACAGGAGGAAAGAGCAU | |
Cyclo | 67 | SEQ. ID 0098 | | |
Cyclo | ||||
68 | SEQ. ID 0099 | AGGAGGAAAGAGCAUCUAC | ||
Cyclo | 69 | SEQ. ID 0100 | | |
Cyclo | ||||
70 | SEQ. ID 0101 | GGAAAGAGCAUCUACGGUG | ||
Cyclo | 71 | SEQ. ID 0102 | AAAGAGCAUCUACGGUGAG | |
Cyclo | 72 | SEQ. ID 0103 | AGAGCAUCUACGGUGAGCG | |
Cyclo | 73 | SEQ. ID 0104 | AGCAUCUACGGUGAGCGCU | |
Cyclo | 74 | SEQ. ID 0105 | CAUCUACGGUGAGCGCUUC | |
Cyclo | 75 | SEQ. ID 0106 | UCUACGGUGAGCGCUUCCC | |
Cyclo | 76 | SEQ. ID 0107 | UACGGUGAGCGCUUCCCCG | |
Cyclo | 77 | SEQ. ID 0108 | CGGUGAGCGCUUCCCCGAU | |
Cyclo | 78 | SEQ. ID 0109 | GUGAGCGCUUCCCCGAUGA | |
Cyclo | 79 | SEQ. ID 0110 | | |
Cyclo | ||||
80 | SEQ. ID 0111 | | ||
Cyclo | ||||
81 | SEQ. ID 0112 | GCUUCCCCGAUGAGAACUU | ||
Cyclo | 82 | SEQ. ID 0113 | UUCCCCGAUGAGAACUUCA | |
Cyclo | 83 | SEQ. ID 0114 | CCCCGAUGAGAACUUCAAA | |
Cyclo | 84 | SEQ. ID 0115 | CCGAUGAGAACUUCAAACU | |
Cyclo | 85 | SEQ. ID 0116 | GAUGAGAACUUCAAACUGA | |
Cyclo | 86 | SEQ. ID 0117 | UGAGAACUUCAAACUGAAG | |
Cyclo | 87 | SEQ. ID 0118 | AGAACUUCAAACUGAAGCA | |
Cyclo | 88 | SEQ. ID 0119 | AACUUCAAACUGAAGCACU | |
Cyclo | 89 | SEQ. ID 0120 | | |
Cyclo | ||||
90 | SEQ. ID 0121 | | ||
DB | ||||
1 | SEQ. ID 0122 | | ||
DB | ||||
2 | SEQ. ID 0123 | | ||
DB | ||||
3 | SEQ. ID 0124 | | ||
DB | ||||
4 | SEQ. ID 0125 | | ||
DB | ||||
5 | SEQ. ID 0126 | | ||
DB | ||||
6 | SEQ. ID 0127 | | ||
DB | ||||
7 | SEQ. ID 0128 | | ||
DB | ||||
8 | SEQ. ID 0129 | | ||
DB | ||||
9 | SEQ. ID 0130 | | ||
DB | ||||
10 | SEQ. ID 0131 | | ||
DB | ||||
11 | SEQ. ID 0132 | | ||
DB | ||||
12 | SEQ. ID 0133 | | ||
DB | ||||
13 | SEQ. ID 0134 | | ||
DB | ||||
14 | SEQ. ID 0135 | | ||
DB | ||||
15 | SEQ. ID 0136 | | ||
DB | ||||
16 | SEQ. ID 0137 | | ||
DB | ||||
17 | SEQ. ID 0138 | | ||
DB | ||||
18 | SEQ. ID 0139 | | ||
DB | ||||
19 | SEQ. ID 0140 | | ||
DB | ||||
20 | SEQ. ID 0141 | | ||
DB | ||||
21 | SEQ. ID 0142 | | ||
DB | ||||
22 | SEQ. ID 0143 | | ||
DB | ||||
23 | SEQ. ID 0144 | | ||
DB | ||||
24 | SEQ. ID 0145 | | ||
DB | ||||
25 | SEQ. ID 0146 | | ||
DB | ||||
26 | SEQ. ID 0147 | | ||
DB | ||||
27 | SEQ. ID 0148 | | ||
DB | ||||
28 | SEQ. ID 0149 | | ||
DB | ||||
29 | SEQ. ID 0150 | | ||
DB | ||||
30 | SEQ. ID 0151 | | ||
DB | ||||
31 | SEQ. ID 0152 | | ||
DB | ||||
32 | SEQ. ID 0153 | AGCUGAAAGGGACUUCCAA | ||
DB | 33 | SEQ. ID 0154 | | |
DB | ||||
34 | SEQ. ID 0155 | CUGAAAGGGACUUCCAAGG | ||
DB | 35 | SEQ. ID 0156 | | |
DB | ||||
36 | SEQ. ID 0157 | | ||
DB | ||||
37 | SEQ. ID 0158 | AAAGGGACUUCCAAGGAAG | ||
DB | 38 | SEQ. ID 0159 | AAGGGACUUCCAAGGAAGA | |
DB | 39 | SEQ. ID 0160 | | |
DB | ||||
40 | SEQ. ID 0161 | GGGACUUCCAAGGAAGAUG | ||
DB | 41 | SEQ. ID 0162 | | |
DB | ||||
42 | SEQ. ID 0163 | GACUUCCAAGGAAGAUGCC | ||
DB | 43 | SEQ. ID 0164 | ACUUCCAAGGAAGAUGCCA | |
DB | 44 | SEQ. ID 0165 | CUUCCAAGGAAGAUGCCAU | |
DB | 45 | SEQ. ID 0166 | UUCCAAGGAAGAUGCCAUG | |
DB | 46 | SEQ. ID 0167 | | |
DB | ||||
47 | SEQ. ID 0168 | CCAAGGAAGAUGCCAUGAA | ||
DB | 48 | SEQ. ID 0169 | CAAGGAAGAUGCCAUGAAA | |
DB | 49 | SEQ. ID 0170 | | |
DB | ||||
50 | SEQ. ID 0171 | AGGAAGAUGCCAUGAAAGC | ||
DB | 51 | SEQ. ID 0172 | | |
DB | ||||
52 | SEQ. ID 0173 | GAAGAUGCCAUGAAAGCUU | ||
DB | 53 | SEQ. ID 0174 | AAGAUGCCAUGAAAGCUUA | |
DB | 54 | SEQ. ID 0175 | AGAUGCCAUGAAAGCUUAC | |
DB | 55 | SEQ. ID 0176 | | |
DB | ||||
56 | SEQ. ID 0177 | | ||
DB | ||||
57 | SEQ. ID 0178 | | ||
DB | ||||
58 | SEQ. ID 0179 | GCCAUGAAAGCUUACAUCA | ||
DB | 59 | SEQ. ID 0180 | | |
DB | ||||
60 | SEQ. ID 0181 | CAUGAAAGCUUACAUCAAC | ||
DB | 61 | SEQ. ID 0182 | | |
DB | ||||
62 | SEQ. ID 0183 | | ||
DB | ||||
63 | SEQ. ID 0184 | GAAAGCUUACAUCAACAAA | ||
DB | 64 | SEQ. ID 0185 | AAAGCUUACAUCAACAAAG | |
DB | 65 | SEQ. ID 0186 | AAGCUUACAUCAACAAAGU | |
DB | 66 | SEQ. ID 0187 | AGCUUACAUCAACAAAGUA | |
DB | 67 | SEQ. ID 0188 | | |
DB | ||||
68 | SEQ. ID 0189 | CUUACAUCAACAAAGUAGA | ||
DB | 69 | SEQ. ID 0190 | | |
DB | ||||
70 | SEQ. ID 0191 | UACAUCAACAAAGUAGAAG | ||
DB | 71 | SEQ. ID 0192 | ACAUCAACAAAGUAGAAGA | |
DB | 72 | SEQ. ID 0193 | CAUCAACAAAGUAGAAGAG | |
DB | 73 | SEQ. ID 0194 | AUCAACAAAGUAGAAGAGC | |
DB | 74 | SEQ. ID 0195 | UCAACAAAGUAGAAGAGCU | |
DB | 75 | SEQ. ID 0196 | CAACAAAGUAGAAGAGCUA | |
DB | 76 | SEQ. ID 0197 | AACAAAGUAGAAGAGCUAA | |
DB | 77 | SEQ. ID 0198 | ACAAAGUAGAAGAGCUAAA | |
DB | 78 | SEQ. ID 0199 | CAAAGUAGAAGAGCUAAAG | |
DB | 79 | SEQ. ID 0200 | | |
DB | ||||
80 | SEQ. ID 0201 | | ||
DB | ||||
81 | SEQ. ID 0202 | AGUAGAAGAGCUAAAGAAA | ||
DB | 82 | SEQ. ID 0203 | GUAGAAGAGCUAAAGAAAA | |
DB | 83 | SEQ. ID 0204 | UAGAAGAGCUAAAGAAAAA | |
DB | 84 | SEQ. ID 0205 | AGAAGAGCUAAAGAAAAAA | |
DB | 85 | SEQ. ID 0206 | GAAGAGCUAAAGAAAAAAU | |
DB | 86 | SEQ. ID 0207 | AAGAGCUAAAGAAAAAAUA | |
DB | 87 | SEQ. ID 0208 | AGAGCUAAAGAAAAAAUAC | |
DB | 88 | SEQ. ID 0209 | GAGCUAAAGAAAAAAUACG | |
DB | 89 | SEQ. ID 0210 | | |
DB | ||||
90 | SEQ. ID 0211 | | ||
Luc | ||||
1 | SEQ. ID 0212 | | ||
Luc | ||||
2 | SEQ. ID 0213 | | ||
Luc | ||||
3 | SEQ. ID 0214 | | ||
Luc | ||||
4 | SEQ. ID 0215 | | ||
Luc | ||||
5 | SEQ. ID 0216 | | ||
Luc | ||||
6 | SEQ. ID 0217 | | ||
Luc | ||||
7 | SEQ. ID 0218 | | ||
Luc | ||||
8 | SEQ. ID 0219 | | ||
Luc | ||||
9 | SEQ. ID 0220 | | ||
Luc | ||||
10 | SEQ. ID 0221 | | ||
Luc | ||||
11 | SEQ. ID 0222 | | ||
Luc | ||||
12 | SEQ. ID 0223 | | ||
Luc | ||||
13 | SEQ. ID 0224 | | ||
Luc | ||||
14 | SEQ. ID 0225 | | ||
Luc | ||||
15 | SEQ. ID 0226 | | ||
Luc | ||||
16 | SEQ. ID 0227 | | ||
Luc | ||||
17 | SEQ. ID 0228 | | ||
Luc | ||||
18 | SEQ. ID 0229 | | ||
Luc | ||||
19 | SEQ. ID 0230 | | ||
Luc | ||||
20 | SEQ. ID 0231 | | ||
Luc | ||||
21 | SEQ. ID 0232 | | ||
Luc | ||||
22 | SEQ. ID 0233 | | ||
Luc | ||||
23 | SEQ. ID 0234 | | ||
Luc | ||||
24 | SEQ. ID 0235 | | ||
Luc | ||||
25 | SEQ. ID 0236 | | ||
Luc | ||||
26 | SEQ. ID 0237 | | ||
Luc | ||||
27 | SEQ. ID 0238 | | ||
Luc | ||||
28 | SEQ. ID 0239 | | ||
Luc | ||||
29 | SEQ. ID 0240 | | ||
Luc | ||||
30 | SEQ. ID 0241 | | ||
Luc | ||||
31 | SEQ. ID 0242 | | ||
Luc | ||||
32 | SEQ. ID 0243 | UGUUUGUGGACGAAGUACC | ||
Luc | 33 | SEQ. ID 0244 | | |
Luc | ||||
34 | SEQ. ID 0245 | GUUGUGUUUGUGGACGAAG | ||
Luc | 35 | SEQ. ID 0246 | | |
Luc | ||||
36 | SEQ. ID 0247 | | ||
Luc | ||||
37 | SEQ. ID 0248 | GGAGGAGUUGUGUUUGUGG | ||
Luc | 38 | SEQ. ID 0249 | GCGGAGGAGUUGUGUUUGU | |
Luc | 39 | SEQ. ID 0250 | | |
Luc | ||||
40 | SEQ. ID 0251 | UUGCGCGGAGGAGUUGUGU | ||
Luc | 41 | SEQ. ID 0252 | | |
Luc | ||||
42 | SEQ. ID 0253 | AAAGUUGCGCGGAGGAGUU | ||
Luc | 43 | SEQ. ID 0254 | AAAAAGUUGCGCGGAGGAG | |
Luc | 44 | SEQ. ID 0255 | CGAAAAAGUUGCGCGGAGG | |
Luc | 45 | SEQ. ID 0256 | CGCGAAAAAGUUGCGCGGA | |
Luc | 46 | SEQ. ID 0257 | | |
Luc | ||||
47 | SEQ. ID 0258 | CAACCGCGAAAAAGUUGCG | ||
Luc | 48 | SEQ. ID 0259 | AACAACCGCGAAAAAGUUG | |
Luc | 49 | SEQ. ID 0260 | | |
Luc | ||||
50 | SEQ. ID 0261 | AAGUAACAACCGCGAAAAA | ||
Luc | 51 | SEQ. ID 0262 | | |
Luc | ||||
52 | SEQ. ID 0263 | AGUCAAGUAACAACCGCGA | ||
Luc | 53 | SEQ. ID 0264 | CCAGUCAAGUAACAACCGC | |
Luc | 54 | SEQ. ID 0265 | CGCCAGUCAAGUAACAACC | |
Luc | 55 | SEQ. ID 0266 | | |
Luc | ||||
56 | SEQ. ID 0267 | | ||
Luc | ||||
57 | SEQ. ID 0268 | | ||
Luc | ||||
58 | SEQ. ID 0269 | GAUUACGUCGCCAGUCAAG | ||
Luc | 59 | SEQ. ID 0270 | | |
Luc | ||||
60 | SEQ. ID 0271 | CGUGGAUUACGUCGCCAGU | ||
Luc | 61 | SEQ. ID 0272 | | |
Luc | ||||
62 | SEQ. ID 0273 | | ||
Luc | ||||
63 | SEQ. ID 0274 | AGAGAUCGUGGAUUACGUC | ||
Luc | 64 | SEQ. ID 0275 | AAAGAGAUCGUGGAUUACG | |
Luc | 65 | SEQ. ID 0276 | AAAAAGAGAUCGUGGAUUA | |
Luc | 66 | SEQ. ID 0277 | GGAAAAAGAGAUCGUGGAU | |
Luc | 67 | SEQ. ID 0278 | | |
Luc | ||||
68 | SEQ. ID 0279 | UGACGGAAAAAGAGAUCGU | ||
Luc | 69 | SEQ. ID 0280 | | |
Luc | ||||
70 | SEQ. ID 0281 | ACGAUGACGGAAAAAGAGA | ||
Luc | 71 | SEQ. ID 0282 | AGACGAUCACGGAAAAAGA | |
Luc | 72 | SEQ. ID 0283 | AAAGACGAUGACGGAAAAA | |
Luc | 73 | SEQ. ID 0284 | GGAAAGACGAUGACGGAAA | |
Luc | 74 | SEQ. ID 0285 | ACGGAAAGACGAUGACGGA | |
Luc | 75 | SEQ. ID 0286 | GCACGGAAAGACGAUGACG | |
Luc | 76 | SEQ. ID 0287 | GAGCACGGAAAGACGAUGA | |
Luc | 77 | SEQ. ID 0288 | UGGAGCACGGAAAGACGAU | |
Luc | 78 | SEQ. ID 0289 | UUUGGAGCACGGAAAGACG | |
Luc | 79 | SEQ. ID 0290 | | |
Luc | ||||
80 | SEQ. ID 0291 | | ||
Luc | ||||
81 | SEQ. ID 0292 | UGUUGUUUUGGAGCACGGA | ||
Luc | 82 | SEQ. ID 0293 | GUUGUUGUUUUGGAGCACG | |
Luc | 83 | SEQ. ID 0294 | CCGUUGUUGUUUUGGAGCA | |
Luc | 84 | SEQ. ID 0295 | CGCCGUUGUUGUUUUGGAG | |
Luc | 85 | SEQ. ID 0296 | GCCGCCGUUGUUGUUUUGG | |
Luc | 86 | SEQ. ID 0297 | CCGCCGCCGUUGUUGUUUU | |
Luc | 87 | SEQ. ID 0298 | UCCCGCCGCCGUUGUUGUU | |
Luc | 88 | SEQ. ID 0299 | CUUCCCGCCGCCGUUGUUG | |
Luc | 89 | SEQ. ID 0300 | | |
Luc | ||||
90 | SEQ. ID.0301 | UGAACUUCCCGCCGCCGUU | ||
|
GGGAGAUAGUGAUGAAGUA | SEQ. ID NO. 302 | |
|
GAAGUACAUCCAUUAUAAG | SEQ. ID NO. 303 | |
|
GUACGACAACCGGGAGAUA | SEQ. ID NO. 304 | |
|
AGAUAGUGAUGAAGUACAU | SEQ. ID NO. 305 | |
|
UGAAGACUCUGCUCAGUUU | SEQ. ID NO. 306 | |
|
GCAUGCGGCCUCUGUUUGA | SEQ. ID NO. 307 | |
|
UGCGGCCUCUGUUUGAUUU | SEQ. ID NO. 308 | |
|
GAGAUAGUGAUGAAGUACA | SEQ. ID NO. 309 | |
|
GGAGAUAGUGAUGAAGUAC | SEQ. ID NO. 310 | |
|
GAAGACUCUGCUCAGUUUG | SEQ. ID NO. 311 | |
Bcl2 siRNA: Sense Strand, 5′→3′ |
TABLE V | ||||||
SEQ. | ||||||
ID | FORMULA | FORMULA | ||||
GENE | Name | No. | FTLLSEQTENCE | VIII | IX | |
CLTC | NM_004859 | 0312 | GAAAGAATCTGTAGAGAAA | 76 | 94.2 | |
CLTC | NM_004859 | 0313 | GCAATGAGCTGTTTGAAGA | 65 | 39.9 | |
CLTC | NM_004859 | 0314 | TGACAAAGGTGGATAAATT | 57 | 38.2 | |
CLTC | NM_004859 | 0315 | GGAAATGGATCTCTTTGAA | 54 | 49.4 | |
CLTA | NM_001833 | 0316 | GGAAAGTAATGGTCCAACA | 22 | 55.5 | |
CLTA | NM_001833 | 0317 | AGACAGTTATGCAGCTATT | 4 | 22.9 | |
CLTA | NM_001833 | 0318 | CCAATTCTCGGAAGCAAGA | 1 | 17 | |
CLTA | NM_001833 | 0319 | GAAAGTAATGGTCCAACAG | −1 | −13 | |
CLTB | NM_001834 | 0320 | GCGCCAGAGTGAACAAGTA | 17 | 57.5 | |
CLTB | NM_001834 | 0321 | GAAGGTGGCCCAGCTATGT | 15 | −8.6 | |
CLTB | NM_001834 | 0322 | GGAACCAGCGCCAGAGTGA | 13 | 40.5 | |
CLTB | NM_001834 | 0323 | GAGCGAGATTGCAGGCATA | 20 | 61.7 | |
CALM | U45976 | 0324 | GTTAGTATCTGATGACTTG | 36 | −34.6 | |
CALM | U45976 | 0325 | GAAATGGAACCACTAAGAA | 33 | 46.1 | |
CALM | U45976 | 0326 | GGAAATGGAACCACTAAGA | 30 | 61.2 | |
CALM | U45976 | 0327 | CAACTACACTTTCCAATGC | 28 | 6.8 | |
EPS15 | NM_001981 | 0328 | CCACCAAGATTTCATGATA | 48 | 25.2 | |
EPS15 | NM_001981 | 0329 | GATCGGAACTCCAACAAGA | 43 | 49.3 | |
EPS15 | NM_001981 | 0330 | AAACGGAGCTACAGATTAT | 39 | 11.5 | |
EPS15 | NM_001981 | 0331 | CCACACAGCATTCTTGTAA | 33 | −23.6 | |
EPS15R | NM_021235 | 0332 | GAAGTTACCTTGAGCAATC | 48 | 33 | |
EPS15R | NM_021235 | 0333 | GGACTTGGCCGATCCAGAA | 27 | 33 | |
EPS15R | NM_021235 | 0334 | GCACTTGGATCGAGATGAG | 20 | 1.3 | |
EPS15R | NM_021235 | 0335 | CAAAGACCAATTCGCGTTA | 17 | 27.7 | |
DNM_2 | NM_004945 | 0336 | CCGAATCAATCGCATCTTC | 6 | −29.6 | |
DNM_2 | NM_004945 | 0337 | GACATGATCCTGCAGTTCA | 5 | −14 | |
DNM_2 | NM_004945 | 0338 | GAGCGAATCGTCACCACTT | 5 | 24 | |
DNM_2 | NM_004945 | 0339 | CCTCCGAGCTGGCGTCTAC | −4 | −63.6 | |
ARF6 | AF93885 | 0340 | TCACATGGTTAACCTCTAA | 27 | −21.1 | |
ARF6 | AF93885 | 0341 | GATGAGGGACGCCATAATC | 7 | −38.4 | |
ARF6 | AF93885 | 0342 | CCTCTAACTACAAATCTTA | 4 | 16.9 | |
ARF6 | AF93885 | 0343 | GGAAGGTGCTATCCAAAAT | 4 | 11.5 | |
RAB5A | BC001267 | 0344 | GCAAGCAAGTCCTAACATT | 40 | 25.1 | |
RAB5A | BC001267 | 0345 | GGAAGAGGAGTAGACCTTA | 17 | 50.1 | |
RAB5A | BC001267 | 0346 | AGGAATCAGTGTTGTAGTA | 16 | 11.5 | |
RAB5A | BC001267 | 0347 | GAAGAGGAGTAGACCTTAC | 12 | 7 | |
RAB5B | NM_002868 | 0348 | GAAAGTCAAGCCTGGTATT | 14 | 18.1 | |
RAB5B | NM_002868 | 0349 | AAAGTCAAGCCTGGTATTA | 6 | −17.8 | |
RAB5B | NM_002868 | 0350 | GCTATGAACGTGAATGATC | 3 | −21.1 | |
RAB5B | NM_002868 | 0351 | CAAGCCTGGTATTACGTTT | −7 | −37.5 | |
RAB5C | AF141304 | 0352 | GGAACAAGATCTGTCAATT | 38 | 51.9 | |
RAB5C | AF141304 | 0353 | GCAATGAACGTGAACGAAA | 29 | 43.7 | |
RAB5C | AF141304 | 0354 | CAATGAACGTGAACGAAAT | 18 | 43.3 | |
RAB5C | AF141304 | 0355 | GGACAGGAGCGGTATCACA | 6 | 18.2 | |
EEA1 | XM_018197 | 0356 | AGACAGAGCTTGAGAATAA | 67 | 64.1 | |
EEA1 | XM_018197 | 0357 | GAGAAGATCTTTATGCAAA | 60 | 48.7 | |
EEA1 | XM_018197 | 0358 | GAAGAGAAATCAGCAGATA | 58 | 45.7 | |
EEA1 | XM_018197 | 0359 | GCAAGTAACTCAACTAACA | 56 | 72.3 | |
AP2B1 | NM_001282 | 0360 | GAGCTAATCTGCCACATTG | 49 | −12.4 | |
AP2B1 | NM_001282 | 0361 | GCAGATGAGTTACTAGAAA | 44 | 48.9 | |
AP2B1 | NM_001282 | 0362 | CAACTTAATTGTCCAGAAA | 41 | 28.2 | |
AP2B1 | NM_001282 | 0363 | CAACACAGGATTCTGATAA | 33 | −5.8 | |
PLK | NM_005030 | 0364 | AGATTGTCCCTAAGTCTCT | −35 | −3.4 | |
PLK | NM_005030 | 0365 | ATGAAGATCTGGAGGTGAA | 0 | −4.3 | |
PLK | NM_005030 | 0366 | TTTGAGACTTCTTGCCTAA | −5 | −27.7 | |
PLK | NM_005030 | 0367 | AGATCACCCTCCTTAAATA | 15 | 72.3 | |
GAPDH | NM_002046 | 0368 | CAACGGATTTGGTCGTATT | 27 | −2.8 | |
GAPDH | NM_002046 | 0369 | GAAATCCCATCACCATCTT | 24 | 3.9 | |
GAPDH | NM_002046 | 0370 | GACCTCAACTACATGGTTT | 22 | −22.9 | |
GAPDH | NM_002046 | 0371 | TGGTTTACATGTTCCAATA | 9 | 9.8 | |
c-Myc | 0372 | GAAGAAATCGATGTTGTTT | 31 | −11.7 | ||
c-Myc | 0373 | ACACAAACTTGAACAGCTA | 22 | 51.3 | ||
c-Myc | 0374 | GGAAGAAATCGATGTTGTT | 18 | 26 | ||
c-Myc | 0375 | GAAACGACGAGAACAGTTG | 18 | −8.9 | ||
MAP2K1 | NM_002755 | 0376 | GCACATGGATGGAGGTTCT | 26 | 16 | |
MAP2K1 | NM_002755 | 0377 | GCAGAGAGAGCACATTTGA | 16 | 0.4 | |
MAP2K1 | NM_002755 | 0378 | GAGGTTCTCTGGATCAAGT | 14 | 15.5 | |
MAP2K1 | NM_002755 | 0379 | GAGCAGATTTGAAGCAACT | 14 | 18.5 | |
MAP2K2 | NM_030662 | 0380 | CAAAGACGATGACTTCGAA | 37 | 26.4 | |
MAP2K2 | NM_030662 | 0381 | GATCAGCATTTGCATGGAA | 24 | −0.7 | |
MAP2K2 | NM_030662 | 0382 | TCCAGGAGTTTGTCAATAA | 17 | −4.5 | |
MAP2K2 | NM_030662 | 0383 | GGAAGCTGATCCACCTTGA | 16 | 59.2 | |
KNSL1(EG5) | NM_004523 | 0384 | GCAGAAATCTAAGGATATA | 53 | 35.8 | |
KNSL1(EG5) | NM_004523 | 0385 | CAACAAGGATGAACTCTAT | 50 | 18.3 | |
KNSL1(EG5) | NM_004523 | 0386 | CAGCAGAAATCTAAGGATA | 41 | 32.7 | |
KNSL1(EG5) | NM_004523 | 0387 | CTAGATGGCTTTCTCAGTA | 39 | 3.9 | |
CyclophilinA | NM_021130 | 0388 | AGACAAGGTCCCAAAGACA | −16 | 58.1 | |
CyclophilinA | NM_021130 | 0389 | GGAATGGCAAGACCAGCAA | −6 | 36 | |
CyclophilinA | NM_021130 | 0390 | AGAATTATTCCAGGGTTTA | −3 | 16.1 | |
CyclophilinA | NM_021130 | 0391 | GCAGACAAGGTCCCAAAGA | 8 | 8.9 | |
LAMIN A/C | NM_170707 | 0392 | AGAAGCAGCTTCAGGATGA | 31 | 38.8 | |
LAMIN A/C | NM_170707 | 0393 | GAGCTTGACTTCCAGAAGA | 33 | 22.4 | |
LAMIN A/C | NM_170707 | 0394 | CCACCGAAGTTCACCCTAA | 21 | 27.5 | |
LAMIN A/C | NM_170707 | 0395 | GAGAAGAGCTCCTCCATCA | 55 | 30.1 | |
CyclophilinB | M60857 | 0396 | GAAAGAGCATCTACGGTGA | 41 | 83.9 | |
CyclophilinB | M60857 | 0397 | GAAAGGATTTGGCTACAAA | 53 | 59.1 | |
CyclophilinB | M60857 | 0398 | ACAGCAAATTCCATCGTGT | −20 | 28.8 | |
CyclophilinB | M60857 | 0399 | GGAAAGACTGTTCCAAAAA | 2 | 27 | |
DBI1 | NM_020548 | 0400 | CAACACGCCTCATCCTCTA | 27 | −7.6 | |
DBI2 | NM_020548 | 0401 | CATGAAAGCTTACATCAAC | 25 | −30.8 | |
DBI3 | NM_020548 | 0402 | AAGATGCCATGAAAGCTTA | 17 | 22 | |
DBI4 | NM_020548 | 0403 | GCACATACCGCCTGAGTCT | 15 | 3.9 | |
rLUC1 | 0404 | GATCAAATCTGAAGAAGGA | 57 | 49.2 | ||
rLUC2 | 0405 | GCCAAGAAGTTTCCTAATA | 50 | 13.7 | ||
rLUC3 | 0406 | CAGCATATCTTGAACCATT | 41 | −2.2 | ||
rLUC4 | 0407 | GAACAAAGGAAACGGATGA | 39 | 29.2 | ||
SeAP1 | NM_031313 | 0408 | CGGAAACGGTCCAGGCTAT | 6 | 26.9 | |
SeAP2 | NM_031313 | 0409 | GCTTCGAGCAGACATGATA | 4 | −11.2 | |
SeAP3 | NM_031313 | 0410 | CCTACACGGTCCTCCTATA | 4 | 4.9 | |
SeAP4 | NM_031313 | 0411 | GCCAACAACCTCATCATCT | 1 | −9.9 | |
fLUC1 | 0412 | GATATGGGCTGAATACAAA | 54 | 40.4 | ||
fLUC2 | 0413 | GCACTCTGATTGACAAATA | 47 | 54.7 | ||
fLUC3 | 0414 | TGAAGTCTCTGATTAAGTA | 46 | 34.5 | ||
fLUC4 | 0415 | TCAGAGAGATCCTCATAAA | 40 | 11.4 | ||
mCyclo_1 | NM_008907 | 0416 | GCAAGAAGATCACCATTTC | 52 | 46.4 | |
mCyclo_2 | NM_008907 | 0417 | GAGAGAAATTTGAGGATGA | 36 | 70.7 | |
mCyclo_3 | NM_008907 | 0418 | GAAAGGATTTGGCTATAAG | 35 | −1.5 | |
mCyclo_4 | NM_008907 | 0419 | GAAAGAAGGCATGAACATT | 27 | 10.3 | |
BCL2_1 | NM_000633 | 0420 | GGGAGATAGTGATGAAGTA | 21 | 72 | |
BCL2_2 | NM_000633 | 0421 | GAAGTACATCCATTATAAG | 1 | 3.3 | |
BCL2_3 | NM_000633 | 0422 | GTACGACAACCGGGAGATA | 1 | 35.9 | |
BCL2_4 | NM_000633 | 0423 | AGATAGTGATGAAGTACAT | −12 | 22.1 | |
BCL2_5 | NM_000633 | 0424 | TGAAGACTCTGCTCAGTTT | 36 | 19.1 | |
BCL2_6 | NM_000633 | 0425 | GCATGCGGCCTCTGTTTGA | 5 | −9.7 | |
QB1 | NM_003365.1 | 0426 | GCACACAGCUUACUACAUC | 52 | −4.8 | |
QB2 | NM_003365.1 | 0427 | GAAAUGCCCUGGUAUCUCA | 49 | 22.1 | |
QB3 | NM_003365.1 | 0428 | GAAGGAACGUGAUGUGAUC | 34 | 22.9 | |
QB4 | NM_003365.1 | 0429 | GCACUACUCCUGUGUGUGA | 28 | 20.4 | |
ATE1-1 | NM_007041 | 0430 | GAACCCAGCUGGAGAACUU | 45 | 15.5 | |
ATE1-2 | NM_007041 | 0431 | GAUAUACAGUGUGAUCUUA | 40 | 12.2 | |
ATE1-3 | NM_007041 | 0432 | GUACUACGAUCCUGAUUAU | 37 | 32.9 | |
ATE1-4 | NM_007041 | 0433 | GUGCCGACCUUUACAAUUU | 35 | 18.2 | |
EGFR-1 | NM_005228 | 0434 | GAAGGAAACTGAATTCAAA | 68 | 79.4 | |
EGFR-1 | NM_005228 | 0435 | GGAAATATGTACTACGAAA | 49 | 49.5 | |
EGFR-1 | NM_005228 | 0436 | CCACAAAGCAGTGAATTTA | 41 | 7.6 | |
EGFR-1 | NM_005228 | 0437 | GTAACAAGCTCACGCAGTT | 40 | 25.9 | |
TABLE XI | |||
96-WELL | 24-WELL | ||
MIXTURE 1 (TRANSIT-TKO-PLASMID DILUTION MIXTURE) |
Opti-MEM | 9.3 | μl | 46.5 | μl |
TransIT-TKO (1 μg/μl) | 0.5 | μl | 2.5 | |
MIXTURE | ||||
1 | 10.0 | μl | 50.0 | μl |
FINAL VOLUME |
MIXTURE 2 (SIRNA DILUTION MIXTURE) |
Opti-MEM | 9.0 | μl | 45.0 | μl |
siRNA (1 μM) | 1.0 | μl | 5.0 | |
MIXTURE | ||||
2 | 10.0 | μl | 50.0 | μl |
FINAL VOLUME |
MIXTURE 3 (SIRNA-TRANSFECTION REAGENT MIXTURE) |
|
10 | |
50 | |
Mixture | ||||
2 | 10 | |
50 | |
MIXTURE | ||||
3 | 20 | |
100 | μl |
|
Incubate |
20 minutes at room temperature |
MIXTURE 4 (MEDIA-SIRNA/TRANSFECTION |
REAGENT MIXTURE) |
|
20 | |
100 | μl |
|
80 | μl | 400 | |
MIXTURE | ||||
4 | 100 | μl | 500 | μl |
FINAL VOLUME |
Incubate 48 hours at 37° C. |
siRNA Sense Sequence | Sequence ID Number | ||
AAACUGGCCCUGCGUCAUG | 438 | |||
AAAGAAUGGGAGUGGCAAU | 439 | |||
AAAGCCACUUCUCAUACAA | 440 | |||
AAAGGAAACUCAGUCUUCU | 441 | |||
AAGAAGGAUUGCCAUUAGU | 442 | |||
ACAAGCACAAGACCAAGUU | 443 | |||
ACAAGCAGGUGAUCUUCGA | 444 | |||
ACAUGGAGGCCCUGGUGAA | 445 | |||
ACCAGGAACUAUAUCUUUG | 446 | |||
AGAAGAAGUUCCAGAAAUA | 447 | |||
AGAAUGAAGUGUACAAGCA | 448 | |||
AGACUGAGGUCCUGGAGGU | 449 | |||
AGCAAGACAUCGACCUGUA | 450 | |||
AGGAAACUCAGUCUUCUUG | 451 | |||
AGGCCACAGAAGUGAGCAA | 452 | |||
CAAAGGAAACUCAGUCUUC | 453 | |||
CAACCCAGCAAGACAUCGA | 454 | |||
CAAGAAGUUCCCAGGCAUA | 455 | |||
CAAGGGCUUUGUGAAACUG | 456 | |||
CACAGAGGCCAGAGAAGUG | 457 | |||
CACCAGGAACUAUAUCUUU | 458 | |||
CAGAAGAAGUUCCAGAAAU | 459 | |||
CAGAAGUGAGCAAGAAGUU | 460 | |||
CAGCACAGAGGCCACAGAA | 461 | |||
CAGCUAACCUCUCUUCUUC | 462 | |||
CAGGAACUAUAUCUUUGGA | 463 | |||
CAGGACCUCUUCUCUGUCA | 464 | |||
CAUACAAGCCCCUUUAUUG | 465 | |||
CCAACCCUCUCCUGUGUGU | 466 | |||
CCAAGCCCAUCACCACUGU | 467 | |||
CCACAGAAGUGAGCAAGAA | 468 | |||
CCACCAGGAACUAUAUCUU | 469 | |||
CCAGAAGAAGUUCCAGAAA | 470 | |||
CCAGUUAGAUGAUUCACUU | 471 | |||
CCAGUUUGAUCUCCCUUCU | 472 | |||
CCAUCCAGCUGGUGAAGAC | 473 | |||
CCGCAAGGGCUUUGUGAAA | 474 | |||
CCUGAUGUCUGGAGGUAUC | 475 | |||
CGAAAGCCACUUCUCAUAC | 476 | |||
CGCAAGGGCUUUGUGAAAC | 477 | |||
CUACUUUCGAGACUACUUU | 478 | |||
CUGAGGUCCUGGAGGUGAA | 479 | |||
GAAACUGGCCCUGCGUCAU | 480 | |||
GAAAGCCACUUCUCAUACA | 481 | |||
GAACACACCCAAGAAAGGU | 482 | |||
GAACCCAAGCCUCACUUUU | 483 | |||
GAACCGCAAGGGCUUUGUG | 484 | |||
GAAGAAGGAUUGCCAUUAG | 485 | |||
GAAGAAGUUCCAGAAAUAC | 486 | |||
GAAGGAUUGCCAUUAGUGA | 487 | |||
GAAGUUCCAGAAAUACAUU | 488 | |||
GAAUGGGAGUGGCAAUGCU | 489 | |||
GACAAGCACAAGACCAAGU | 490 | |||
GACUGGAACACACCCAAGA | 491 | |||
GAGACUACUUUCCCAUCCA | 492 | |||
GAGAGAAUGAAGUGUACAA | 493 | |||
GAGAGGCGAAAGCCACUUC | 494 | |||
GAGGAUGCCGCCACCAUGA | 495 | |||
GAGUGGCAAUGCUAUCAUC | 496 | |||
GAUGGGUCCAGAAGAAGUU | 497 | |||
GCAAGAAGUUCCCAGGCAU | 498 | |||
GCAAGACAUCGACCUGUAC | 499 | |||
GCAAGGGCUUUGUGAAACU | 500 | |||
GCAAUGCUAUCAUCAUCGU | 501 | |||
GCACAGAGGCCACAGAAGU | 502 | |||
GCAGAUUAGUUCCAAAGCA | 503 | |||
GCAGCAGAUUAGUUCCAAA | 504 | |||
GCAGCUACAGGUCAUCUCA | 505 | |||
GCAGGCAACUUCCGAAUGC | 506 | |||
GCAGUGCCAUCCUCAUGUA | 507 | |||
GCCAUCCUCAUGUACAUAU | 508 | |||
GCCCAUCACCACUGUUGUG | 509 | |||
GCCGAUGGGUCCAGAAGAA | 510 | |||
GCCGGGACACCAUAGACUA | 511 | |||
GCGAAAGCCAGUUCUCAUA | 512 | |||
GCGCUACUUUCGAGACUAC | 513 | |||
GCUCAGCUAACCUCUCUUC | 514 | |||
GCUCAUCGCUGUGCUCUAC | 515 | |||
GCUGUGCUCUACUUCACUU | 516 | |||
GGAACACACCCAAGAAAGG | 517 | |||
GGAACCAGCUGCAAAUCAC | 518 | |||
GGAAGAAGGAUUGCCAUUA | 519 | |||
GGAAUGCUCCAGUUUGAUC | 520 | |||
GGAGGAACCAGCUGCAAAU | 521 | |||
GGAGGUAUCUGCCCUGUCA | 522 | |||
GGAGUUGGAGAGCACAGUU | 523 | |||
GGAUUGCCAUUAGUGACUU | 524 | |||
GGCCUUACCUGGCUACACU | 525 | |||
GGCUAAAUCUGGGCCUAAU | 526 | |||
GGGAAGAAGGAUUGCCAUU | 527 | |||
GGGAGUGGCAAUGCUAUCA | 528 | |||
GGGUGUCUGUGGGUUAUUU | 529 | |||
GGUCACAGUGGGUCCGAAA | 530 | |||
GGUCCUGUCCUUCCUUGUA | 531 | |||
GUACAAGCAGGUGAUCUUC | 532 | |||
GUACAUAUUCUGCACUGAU | 533 | |||
GUAGUAGGCAUCUGGAAUG | 534 | |||
GUCAGUAUUUCAAGUUCUU | 535 | |||
GUCCGAAACUGGGCUGUGU | 536 | |||
GUGCCAUCCUCAUGUACAU | 537 | |||
GUUCCCAUCUACUCCUUUG | 538 | |||
GUUCUGUUAUCUCUUGAUG | 539 | |||
GUUGAGGGAGUACCUGAUG | 540 | |||
UAAAUGAAAGUGAGAAUCC | 541 | |||
UACAUAUUCUGCACUGAUU | 542 | |||
UAGGUGGAGUAACUGGUUU | 543 | |||
UCAAAGAAUGGGAGUGGCA | 544 | |||
UCACUUGGCUGGUGUUUGA | 545 | |||
UCAGCUAACCUCUCUUCUU | 546 | |||
UCAUCGCUGUGCUCUACUU | 547 | |||
UCAUGGAGCUGACCUGGUU | 548 | |||
UCCAGGACCUCUUCUCUGU | 549 | |||
UCGACAAGCACAAGACCAA | 550 | |||
UCUAGGUGGUGGCUAAAUC | 551 | |||
UCUCCUGUGUGUUCUGUUA | 552 | |||
UCUGUCACCUGGCUCAAUA | 553 | |||
UCUGUUAUCUCUUGAUGAG | 554 | |||
UGACCACCAGGAACUAUAU | 555 | |||
UGACCUGGUUCCCAUCUAC | 556 | |||
UGAGAUCAUUGCACCAUGU | 557 | |||
UGAGCUAGGUGGAGUAACU | 558 | |||
UGGAGAGAAUGAAGUGUAC | 559 | |||
UGGCAAUGCUAUCAUCAUC | 560 | |||
UGUACAAGCAGGUGAUCUU | 561 | |||
UGUGUUCUGUUAUCUCUUG | 562 | |||
UGUUCUAGGUGGUGGCUAA | 563 | |||
UUAGUGACUUGGACCAGUU | 564 | |||
UUCCAGAAAUACAUUGGUU | 565 | |||
UUGGAGAGAAUGAAGUGUA | 566 | |||
UUGGGUGGCUGAUGACAUG | 567 | |||
UUUCUUGGGUGGCUGAUGA | 568 | |||
Claims (20)
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US12/660,582 US8247169B2 (en) | 2002-11-14 | 2010-03-01 | SiRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) |
US13/539,630 US20120270751A1 (en) | 2002-11-14 | 2012-07-02 | siRNA Targeting Diacylglycerol O-Acyltransferase Homolog 2 (DGAT2) |
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US50205003P | 2003-09-10 | 2003-09-10 | |
US10/714,333 US8090542B2 (en) | 2002-11-14 | 2003-11-14 | Functional and hyperfunctional siRNA |
PCT/US2004/014885 WO2006006948A2 (en) | 2002-11-14 | 2004-05-12 | METHODS AND COMPOSITIONS FOR SELECTING siRNA OF IMPROVED FUNCTIONALITY |
US10/940,892 US20120052487A9 (en) | 2002-11-14 | 2004-09-14 | Methods and compositions for selecting sirna of improved functionality |
US11/978,398 US7709629B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) |
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US10/940,892 Continuation-In-Part US20120052487A9 (en) | 2002-11-14 | 2004-09-14 | Methods and compositions for selecting sirna of improved functionality |
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US10/940,892 Continuation US20120052487A9 (en) | 2002-11-14 | 2004-09-14 | Methods and compositions for selecting sirna of improved functionality |
US12/660,582 Division US8247169B2 (en) | 2002-11-14 | 2010-03-01 | SiRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) |
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Family Applications (118)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/593,100 Expired - Fee Related US7615541B2 (en) | 2002-11-14 | 2006-11-03 | siRNA targeting TIE-2 |
US11/594,530 Expired - Fee Related US7608706B2 (en) | 2002-11-14 | 2006-11-08 | siRNA targeting ras-related nuclear protein |
US11/594,666 Abandoned US20070128641A1 (en) | 2002-11-14 | 2006-11-08 | siRNA targeting hypoxia-inducible factor 1 |
US11/598,179 Expired - Fee Related US7541453B2 (en) | 2002-11-14 | 2006-11-09 | siRNA targeting aquaporin 4 |
US11/595,698 Expired - Fee Related US7598369B2 (en) | 2002-11-14 | 2006-11-09 | siRNA targeting histamine receptor H1 |
US11/729,388 Abandoned US20070185317A1 (en) | 2002-11-14 | 2007-03-28 | siRNA targeting HtrA serine peptidase 1 |
US11/729,924 Abandoned US20080015114A1 (en) | 2002-11-14 | 2007-03-29 | siRNA targeting connective tissue growth factor (CTGF) |
US11/731,843 Expired - Fee Related US7569684B2 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting gremlin |
US11/731,875 Abandoned US20070299253A1 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting vacuolar ATPase |
US11/731,890 Abandoned US20080045703A1 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting platelet-derived growth factor receptor beta polypeptide (PDGFR) |
US11/731,894 Expired - Fee Related US7521191B2 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting connexin 43 |
US11/732,413 Abandoned US20070238868A1 (en) | 2002-11-14 | 2007-04-03 | siRNA targeting chemokine (C-X-C motif) receptor 4 (CXCR4) |
US11/732,457 Expired - Fee Related US7638621B2 (en) | 2002-11-14 | 2007-04-03 | siRNA targeting insulin-like growth factor 1 receptor (IGF-1R) |
US11/732,809 Abandoned US20070255046A1 (en) | 2002-11-14 | 2007-04-04 | siRNA targeting spectrin SH3 domain binding protein 1 (SSH3BP1) |
US11/732,810 Abandoned US20070219362A1 (en) | 2002-11-14 | 2007-04-04 | siRNA targeting azurocidin 1 (Cartionic Antimicrobial protein 37) |
US11/784,536 Abandoned US20070179286A1 (en) | 2002-11-14 | 2007-04-06 | siRNA targeting testis-specific serine kinase 4 |
US11/784,559 Abandoned US20070213520A1 (en) | 2002-11-14 | 2007-04-06 | siRNA targeting calcium/calmodulin dependent protein kinase IV (CAMK4) |
US11/784,752 Abandoned US20070213521A1 (en) | 2002-11-14 | 2007-04-09 | siRNA targeting cell division cycle 25 (CDC25C) |
US11/784,756 Abandoned US20070232797A1 (en) | 2002-11-14 | 2007-04-09 | siRNA targeting cell division cycle 2-like 5(CDC2L5) |
US11/784,755 Expired - Fee Related US7550572B2 (en) | 2002-11-14 | 2007-04-09 | siRNA targeting cell division cycle 20 homolog (CDC20) |
US11/807,577 Abandoned US20070260048A1 (en) | 2002-11-14 | 2007-05-29 | siRNA targeting centromere protein E, 312kDa (CENPE) |
US11/807,486 Active 2024-07-25 US7745610B2 (en) | 2002-11-14 | 2007-05-29 | siRNA targeting cyclin dependent kinase 11 (CDK11) |
US11/807,530 Abandoned US20070255047A1 (en) | 2002-11-14 | 2007-05-29 | siRNA targeting cell division cycle 6 homolog (CDC6) |
US11/809,909 Abandoned US20070255048A1 (en) | 2002-11-14 | 2007-06-01 | siRNA targeting discoidin domain receptor family, member 1 (DDR1) |
US11/810,074 Abandoned US20070276135A1 (en) | 2002-11-14 | 2007-06-04 | siRNA targeting dual specificity phosphate 5 (DUSP5) |
US11/810,382 Abandoned US20070260047A1 (en) | 2002-11-14 | 2007-06-05 | siRNA targeting EPH receptor A4 (EPHA4) |
US11/810,383 Expired - Fee Related US7595388B2 (en) | 2002-11-14 | 2007-06-05 | siRNA targeting EPH receptor A3 (EPHA3) |
US11/810,384 Abandoned US20070260049A1 (en) | 2002-11-14 | 2007-06-05 | siRNA targeting MAD2 mitotic arrest deficient-like (MAD2L2) |
US11/810,673 Abandoned US20070293664A1 (en) | 2002-11-14 | 2007-06-06 | siRNA targeting minichromosome maintenance deficient 5 (MCM5) |
US11/810,672 Abandoned US20070255050A1 (en) | 2002-11-14 | 2007-06-06 | siRNA targeting minichromosome maintenance deficient 2, mitotin (MCM2) |
US11/811,003 Abandoned US20070287833A1 (en) | 2002-11-14 | 2007-06-07 | siRNA targeting minichromosome maintenance deficient 6 (MCM6) |
US11/811,012 Abandoned US20070260050A1 (en) | 2002-11-14 | 2007-06-07 | siRNA targeting minichromosome maintenance deficient 7 (MCM7) |
US11/811,005 Abandoned US20070265437A1 (en) | 2002-11-14 | 2007-06-07 | siRNA targeting testes development-related NYD-SP21 (NYD-SP21) |
US11/811,424 Abandoned US20070244312A1 (en) | 2002-11-14 | 2007-06-08 | siRNA targeting phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A) |
US11/811,423 Expired - Lifetime US7645870B2 (en) | 2002-11-14 | 2007-06-08 | siRNA targeting proteasome 26S subunit, non-ATPase, 10 (Gankyrin or PSMD10) |
US11/811,209 Abandoned US20080085997A1 (en) | 2002-11-14 | 2007-06-08 | siRNA targeting phosphoinositide-3-kinase, class 2, beta polypeptide (PIK3C2B) |
US11/811,925 Abandoned US20070260051A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting pituitary tumor-transforming 1 (PTTG1) |
US11/811,954 Abandoned US20070249819A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting WEE1 homolog (WEE1) |
US11/811,950 Abandoned US20070260052A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting RAD1 homolog (RAD1) |
US11/811,929 Abandoned US20070255051A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting serine/threonine kinase 22B (STK22B) |
US11/818,547 Abandoned US20070255052A1 (en) | 2002-11-14 | 2007-06-14 | siRNA targeting v-myc myelocytomatosis viral oncogene homolog (MYC) |
US11/818,936 Expired - Lifetime US7598370B2 (en) | 2002-11-14 | 2007-06-15 | siRNA targeting polo-like kinase-1 (PLK-1) |
US11/818,938 Expired - Lifetime US7678896B2 (en) | 2002-11-14 | 2007-06-15 | siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase) |
US11/880,624 Abandoned US20080027215A1 (en) | 2002-11-14 | 2007-07-23 | siRNA targeting vascular endothelial growth factor (VEGF) |
US11/880,965 Expired - Fee Related US7579458B2 (en) | 2002-11-14 | 2007-07-25 | siRNA targeting synuclein, alpha (SNCA-1) |
US11/881,772 Abandoned US20080027216A1 (en) | 2002-11-14 | 2007-07-27 | siRNA targeting sodium channel, voltage-gated, type X, alpha (SCN10A) |
US11/881,767 Abandoned US20080039617A1 (en) | 2002-11-14 | 2007-07-27 | siRNA targeting neuropeptide Y (NPY) |
US11/975,902 Abandoned US20080097091A1 (en) | 2002-11-14 | 2007-10-22 | siRNA targeting TNFalpha |
US11/977,128 Abandoned US20080097092A1 (en) | 2002-11-14 | 2007-10-23 | siRNA targeting kinases |
US11/977,347 Abandoned US20080076908A1 (en) | 2002-11-14 | 2007-10-24 | siRNA targeting nuclear receptors |
US11/977,675 Abandoned US20080071073A1 (en) | 2002-11-14 | 2007-10-25 | siRNA targeting ubiquitin ligases |
US11/977,558 Abandoned US20080097089A1 (en) | 2002-11-14 | 2007-10-25 | siRNA targeting deubiqutination enzymes |
US11/978,125 Abandoned US20080086002A1 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting secreted frizzled-related protein 1 (sFRP1) |
US11/978,097 Expired - Fee Related US7638622B2 (en) | 2002-11-14 | 2007-10-26 | SiRNA targeting intercellular adhesion molecule 1 (ICAM1) |
US11/978,120 Abandoned US20080081904A1 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting carbonic anhydrase 4(CA4) |
US11/978,070 Expired - Fee Related US7582746B2 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting complement component 3 (C3) |
US11/978,106 Expired - Lifetime US7655789B2 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting transient receptor potential cation channel, subfamily V, member 1 (TRPV1) |
US11/978,107 Expired - Fee Related US7605252B2 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting kinase insert domain receptor (KDR) |
US11/978,398 Expired - Lifetime US7709629B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) |
US11/978,475 Abandoned US20080113372A1 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting glucagon receptor (GCGR) |
US11/978,457 Abandoned US20080113371A1 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting beta secretase (BACE) |
US11/978,518 Expired - Fee Related US7632938B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting superoxide dismutase 1 (SOD1) |
US11/978,487 Abandoned US20080113374A1 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting fructose-1,6-bisphosphatase 1 (FBP1) |
US11/978,455 Expired - Fee Related US7795421B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting apolipoprotein B (APOB) |
US11/978,476 Expired - Fee Related US7635771B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US11/980,102 Expired - Fee Related US7662950B2 (en) | 2002-11-14 | 2007-10-30 | siRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US11/980,300 Expired - Fee Related US7592443B2 (en) | 2002-11-14 | 2007-10-30 | siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4) |
US11/980,263 Expired - Fee Related US7632939B2 (en) | 2002-11-14 | 2007-10-30 | siRNA targeting proto-oncogene MET |
US12/321,749 Expired - Fee Related US7666853B2 (en) | 2002-11-14 | 2009-01-23 | siRNA targeting connective tissue growth factor (CTGF) |
US12/322,387 Expired - Fee Related US7589191B2 (en) | 2002-11-14 | 2009-02-02 | siRNA targeting hypoxia-inducible factor 1 |
US12/455,098 Expired - Fee Related US7741470B2 (en) | 2002-11-14 | 2009-05-28 | siRNA targeting gremlin |
US12/460,876 Abandoned US20100004141A1 (en) | 2002-11-14 | 2009-07-24 | siRNA targeting polo-like Kinase-1 (PLK-1) |
US12/462,029 Expired - Fee Related US7745612B2 (en) | 2002-11-14 | 2009-07-28 | siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4) |
US12/462,420 Expired - Fee Related US7737267B2 (en) | 2002-11-14 | 2009-08-04 | siRNA targeting hypoxia-inducible factor 1 |
US12/462,820 Expired - Fee Related US8022198B2 (en) | 2002-11-14 | 2009-08-10 | siRNA targeting histamine receptor H1 |
US12/584,352 Expired - Fee Related US8222395B2 (en) | 2002-11-14 | 2009-09-03 | siRNA targeting kinase insert domain receptor (KDR) |
US12/584,850 Expired - Fee Related US7897754B2 (en) | 2002-11-14 | 2009-09-11 | SiRNA targeting ras-related nuclear protein RAN |
US12/586,167 Expired - Fee Related US7855186B2 (en) | 2002-11-14 | 2009-09-17 | siRNA targeting TIE-2 |
US12/589,879 Expired - Fee Related US8039610B2 (en) | 2002-11-14 | 2009-10-29 | siRNA targeting superoxide dismutase 1 (SOD1) |
US12/590,097 Expired - Fee Related US7816512B2 (en) | 2002-11-14 | 2009-11-02 | siRNA targeting proto-oncogene MET |
US12/590,252 Expired - Fee Related US7829696B2 (en) | 2002-11-14 | 2009-11-04 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US12/592,872 Active 2024-09-09 US8304528B2 (en) | 2002-11-14 | 2009-12-03 | SiRNA targeting fructose-1, 6-bisphosphatase 1 (FBP1) |
US12/653,120 Expired - Fee Related US8022199B2 (en) | 2002-11-14 | 2009-12-08 | SiRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US12/653,402 Expired - Fee Related US7807820B2 (en) | 2002-11-14 | 2009-12-11 | siRNA targeting beta secretase (BACE) |
US12/655,107 Expired - Fee Related US7833989B2 (en) | 2002-11-14 | 2009-12-23 | siRNA targeting connective tissue growth factor (CTGF) |
US12/657,448 Expired - Lifetime US8067576B2 (en) | 2002-11-14 | 2010-01-21 | siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase) |
US12/660,582 Expired - Lifetime US8247169B2 (en) | 2002-11-14 | 2010-03-01 | SiRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) |
US12/798,603 Expired - Fee Related US8030476B2 (en) | 2002-11-14 | 2010-04-07 | siRNA targeting gremlin |
US12/798,802 Expired - Fee Related US7935813B2 (en) | 2002-11-14 | 2010-04-12 | siRNA target hypoxia-inducible factor 1 |
US12/798,906 Active 2024-06-21 US8236942B2 (en) | 2002-11-14 | 2010-04-13 | SiRNA targeting glucagon receptor (GCGR) |
US12/799,758 Expired - Lifetime US8217162B2 (en) | 2002-11-14 | 2010-04-30 | siRNA targeting interleukin-1 receptor-associated kinase 4(IRAK4) |
US12/799,975 Abandoned US20100267587A1 (en) | 2002-11-14 | 2010-05-05 | siRNA targeting cyclin dependent kinase 11 (CDK11) |
US12/804,014 Expired - Fee Related US8071754B2 (en) | 2002-11-14 | 2010-07-12 | siRNA targeting apolipoprotein B (APOB) |
US12/806,570 Expired - Fee Related US7999097B2 (en) | 2002-11-14 | 2010-08-17 | siRNA targeting beta secretase (BACE) |
US12/807,526 Expired - Fee Related US8222396B2 (en) | 2002-11-14 | 2010-09-08 | SiRNA targeting proto-oncogene MET |
US12/924,078 Expired - Lifetime US8268985B2 (en) | 2002-11-14 | 2010-09-20 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US12/924,653 Expired - Lifetime US8138329B2 (en) | 2002-11-14 | 2010-10-01 | siRNA targeting connective tissue growth factor (CTGF) |
US12/927,144 Expired - Fee Related US8314229B2 (en) | 2002-11-14 | 2010-11-08 | siRNA targeting tie-2 |
US13/135,336 Expired - Lifetime US8293887B2 (en) | 2002-11-14 | 2011-07-01 | SiRNA targeting beta secretase (BACE) |
US13/136,780 Expired - Fee Related US8633306B2 (en) | 2002-11-14 | 2011-08-10 | SiRNA targeting histamine receptor H1 |
US13/136,812 Expired - Fee Related US8426579B2 (en) | 2002-11-14 | 2011-08-11 | SiRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US13/199,240 Abandoned US20110319297A1 (en) | 2002-11-14 | 2011-08-23 | siRNA targeting gremlin |
US13/199,946 Abandoned US20120015850A1 (en) | 2002-11-14 | 2011-09-13 | siRNA targeting Superoxide |
US13/317,752 Expired - Lifetime US8232386B2 (en) | 2002-11-14 | 2011-10-27 | SiRNA targeting apolipoprotein B (APOB) |
US13/385,320 Expired - Fee Related US8461326B2 (en) | 2002-11-14 | 2012-02-14 | SiRNA targeting connective tissue growth factor (CTGF) |
US13/489,725 Abandoned US20120252873A1 (en) | 2002-11-14 | 2012-06-06 | siRNA Targeting Interleukin-1 Receptor-associated Kinase 4 (IRAK4) |
US13/494,360 Abandoned US20120258888A1 (en) | 2002-11-14 | 2012-06-12 | siRNA Targeting Proto-oncogene MET |
US13/524,015 Expired - Fee Related US8575329B2 (en) | 2002-11-14 | 2012-06-15 | siRNA targeting kinase insert domain receptor (KDR) |
US13/536,005 Expired - Fee Related US8445668B2 (en) | 2002-11-14 | 2012-06-28 | SiRNA targeting apolipoprotein (APOB) |
US13/539,630 Abandoned US20120270751A1 (en) | 2002-11-14 | 2012-07-02 | siRNA Targeting Diacylglycerol O-Acyltransferase Homolog 2 (DGAT2) |
US13/542,332 Abandoned US20120283311A1 (en) | 2002-11-14 | 2012-07-05 | siRNA Targeting Glucagon Receptor (GCCR) |
US13/551,794 Expired - Fee Related US8658784B2 (en) | 2002-11-14 | 2012-07-18 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US13/613,910 Abandoned US20130023446A1 (en) | 2002-11-14 | 2012-09-13 | siRNA Targeting Beta Secretase (BACE) |
US13/632,519 Abandoned US20130059760A1 (en) | 2002-11-14 | 2012-10-01 | siRNA Targeting Fructose-1, 6-bisphosphatase 1 (FBP1) |
US13/647,869 Expired - Fee Related US8658785B1 (en) | 2002-11-14 | 2012-10-09 | siRNA targeting tie-2 |
US13/847,544 Expired - Fee Related US8883998B2 (en) | 2002-11-14 | 2013-03-20 | siRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US13/867,175 Abandoned US20130225447A1 (en) | 2002-11-14 | 2013-04-22 | siRNA Targeting Apolipoprotein B (APOB) |
US14/099,339 Expired - Fee Related US8907077B2 (en) | 2002-11-14 | 2013-12-06 | siRNA targeting TIE-2 |
Family Applications Before (58)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/593,100 Expired - Fee Related US7615541B2 (en) | 2002-11-14 | 2006-11-03 | siRNA targeting TIE-2 |
US11/594,530 Expired - Fee Related US7608706B2 (en) | 2002-11-14 | 2006-11-08 | siRNA targeting ras-related nuclear protein |
US11/594,666 Abandoned US20070128641A1 (en) | 2002-11-14 | 2006-11-08 | siRNA targeting hypoxia-inducible factor 1 |
US11/598,179 Expired - Fee Related US7541453B2 (en) | 2002-11-14 | 2006-11-09 | siRNA targeting aquaporin 4 |
US11/595,698 Expired - Fee Related US7598369B2 (en) | 2002-11-14 | 2006-11-09 | siRNA targeting histamine receptor H1 |
US11/729,388 Abandoned US20070185317A1 (en) | 2002-11-14 | 2007-03-28 | siRNA targeting HtrA serine peptidase 1 |
US11/729,924 Abandoned US20080015114A1 (en) | 2002-11-14 | 2007-03-29 | siRNA targeting connective tissue growth factor (CTGF) |
US11/731,843 Expired - Fee Related US7569684B2 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting gremlin |
US11/731,875 Abandoned US20070299253A1 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting vacuolar ATPase |
US11/731,890 Abandoned US20080045703A1 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting platelet-derived growth factor receptor beta polypeptide (PDGFR) |
US11/731,894 Expired - Fee Related US7521191B2 (en) | 2002-11-14 | 2007-03-30 | siRNA targeting connexin 43 |
US11/732,413 Abandoned US20070238868A1 (en) | 2002-11-14 | 2007-04-03 | siRNA targeting chemokine (C-X-C motif) receptor 4 (CXCR4) |
US11/732,457 Expired - Fee Related US7638621B2 (en) | 2002-11-14 | 2007-04-03 | siRNA targeting insulin-like growth factor 1 receptor (IGF-1R) |
US11/732,809 Abandoned US20070255046A1 (en) | 2002-11-14 | 2007-04-04 | siRNA targeting spectrin SH3 domain binding protein 1 (SSH3BP1) |
US11/732,810 Abandoned US20070219362A1 (en) | 2002-11-14 | 2007-04-04 | siRNA targeting azurocidin 1 (Cartionic Antimicrobial protein 37) |
US11/784,536 Abandoned US20070179286A1 (en) | 2002-11-14 | 2007-04-06 | siRNA targeting testis-specific serine kinase 4 |
US11/784,559 Abandoned US20070213520A1 (en) | 2002-11-14 | 2007-04-06 | siRNA targeting calcium/calmodulin dependent protein kinase IV (CAMK4) |
US11/784,752 Abandoned US20070213521A1 (en) | 2002-11-14 | 2007-04-09 | siRNA targeting cell division cycle 25 (CDC25C) |
US11/784,756 Abandoned US20070232797A1 (en) | 2002-11-14 | 2007-04-09 | siRNA targeting cell division cycle 2-like 5(CDC2L5) |
US11/784,755 Expired - Fee Related US7550572B2 (en) | 2002-11-14 | 2007-04-09 | siRNA targeting cell division cycle 20 homolog (CDC20) |
US11/807,577 Abandoned US20070260048A1 (en) | 2002-11-14 | 2007-05-29 | siRNA targeting centromere protein E, 312kDa (CENPE) |
US11/807,486 Active 2024-07-25 US7745610B2 (en) | 2002-11-14 | 2007-05-29 | siRNA targeting cyclin dependent kinase 11 (CDK11) |
US11/807,530 Abandoned US20070255047A1 (en) | 2002-11-14 | 2007-05-29 | siRNA targeting cell division cycle 6 homolog (CDC6) |
US11/809,909 Abandoned US20070255048A1 (en) | 2002-11-14 | 2007-06-01 | siRNA targeting discoidin domain receptor family, member 1 (DDR1) |
US11/810,074 Abandoned US20070276135A1 (en) | 2002-11-14 | 2007-06-04 | siRNA targeting dual specificity phosphate 5 (DUSP5) |
US11/810,382 Abandoned US20070260047A1 (en) | 2002-11-14 | 2007-06-05 | siRNA targeting EPH receptor A4 (EPHA4) |
US11/810,383 Expired - Fee Related US7595388B2 (en) | 2002-11-14 | 2007-06-05 | siRNA targeting EPH receptor A3 (EPHA3) |
US11/810,384 Abandoned US20070260049A1 (en) | 2002-11-14 | 2007-06-05 | siRNA targeting MAD2 mitotic arrest deficient-like (MAD2L2) |
US11/810,673 Abandoned US20070293664A1 (en) | 2002-11-14 | 2007-06-06 | siRNA targeting minichromosome maintenance deficient 5 (MCM5) |
US11/810,672 Abandoned US20070255050A1 (en) | 2002-11-14 | 2007-06-06 | siRNA targeting minichromosome maintenance deficient 2, mitotin (MCM2) |
US11/811,003 Abandoned US20070287833A1 (en) | 2002-11-14 | 2007-06-07 | siRNA targeting minichromosome maintenance deficient 6 (MCM6) |
US11/811,012 Abandoned US20070260050A1 (en) | 2002-11-14 | 2007-06-07 | siRNA targeting minichromosome maintenance deficient 7 (MCM7) |
US11/811,005 Abandoned US20070265437A1 (en) | 2002-11-14 | 2007-06-07 | siRNA targeting testes development-related NYD-SP21 (NYD-SP21) |
US11/811,424 Abandoned US20070244312A1 (en) | 2002-11-14 | 2007-06-08 | siRNA targeting phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A) |
US11/811,423 Expired - Lifetime US7645870B2 (en) | 2002-11-14 | 2007-06-08 | siRNA targeting proteasome 26S subunit, non-ATPase, 10 (Gankyrin or PSMD10) |
US11/811,209 Abandoned US20080085997A1 (en) | 2002-11-14 | 2007-06-08 | siRNA targeting phosphoinositide-3-kinase, class 2, beta polypeptide (PIK3C2B) |
US11/811,925 Abandoned US20070260051A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting pituitary tumor-transforming 1 (PTTG1) |
US11/811,954 Abandoned US20070249819A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting WEE1 homolog (WEE1) |
US11/811,950 Abandoned US20070260052A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting RAD1 homolog (RAD1) |
US11/811,929 Abandoned US20070255051A1 (en) | 2002-11-14 | 2007-06-12 | siRNA targeting serine/threonine kinase 22B (STK22B) |
US11/818,547 Abandoned US20070255052A1 (en) | 2002-11-14 | 2007-06-14 | siRNA targeting v-myc myelocytomatosis viral oncogene homolog (MYC) |
US11/818,936 Expired - Lifetime US7598370B2 (en) | 2002-11-14 | 2007-06-15 | siRNA targeting polo-like kinase-1 (PLK-1) |
US11/818,938 Expired - Lifetime US7678896B2 (en) | 2002-11-14 | 2007-06-15 | siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase) |
US11/880,624 Abandoned US20080027215A1 (en) | 2002-11-14 | 2007-07-23 | siRNA targeting vascular endothelial growth factor (VEGF) |
US11/880,965 Expired - Fee Related US7579458B2 (en) | 2002-11-14 | 2007-07-25 | siRNA targeting synuclein, alpha (SNCA-1) |
US11/881,772 Abandoned US20080027216A1 (en) | 2002-11-14 | 2007-07-27 | siRNA targeting sodium channel, voltage-gated, type X, alpha (SCN10A) |
US11/881,767 Abandoned US20080039617A1 (en) | 2002-11-14 | 2007-07-27 | siRNA targeting neuropeptide Y (NPY) |
US11/975,902 Abandoned US20080097091A1 (en) | 2002-11-14 | 2007-10-22 | siRNA targeting TNFalpha |
US11/977,128 Abandoned US20080097092A1 (en) | 2002-11-14 | 2007-10-23 | siRNA targeting kinases |
US11/977,347 Abandoned US20080076908A1 (en) | 2002-11-14 | 2007-10-24 | siRNA targeting nuclear receptors |
US11/977,675 Abandoned US20080071073A1 (en) | 2002-11-14 | 2007-10-25 | siRNA targeting ubiquitin ligases |
US11/977,558 Abandoned US20080097089A1 (en) | 2002-11-14 | 2007-10-25 | siRNA targeting deubiqutination enzymes |
US11/978,125 Abandoned US20080086002A1 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting secreted frizzled-related protein 1 (sFRP1) |
US11/978,097 Expired - Fee Related US7638622B2 (en) | 2002-11-14 | 2007-10-26 | SiRNA targeting intercellular adhesion molecule 1 (ICAM1) |
US11/978,120 Abandoned US20080081904A1 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting carbonic anhydrase 4(CA4) |
US11/978,070 Expired - Fee Related US7582746B2 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting complement component 3 (C3) |
US11/978,106 Expired - Lifetime US7655789B2 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting transient receptor potential cation channel, subfamily V, member 1 (TRPV1) |
US11/978,107 Expired - Fee Related US7605252B2 (en) | 2002-11-14 | 2007-10-26 | siRNA targeting kinase insert domain receptor (KDR) |
Family Applications After (59)
Application Number | Title | Priority Date | Filing Date |
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US11/978,475 Abandoned US20080113372A1 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting glucagon receptor (GCGR) |
US11/978,457 Abandoned US20080113371A1 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting beta secretase (BACE) |
US11/978,518 Expired - Fee Related US7632938B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting superoxide dismutase 1 (SOD1) |
US11/978,487 Abandoned US20080113374A1 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting fructose-1,6-bisphosphatase 1 (FBP1) |
US11/978,455 Expired - Fee Related US7795421B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting apolipoprotein B (APOB) |
US11/978,476 Expired - Fee Related US7635771B2 (en) | 2002-11-14 | 2007-10-29 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US11/980,102 Expired - Fee Related US7662950B2 (en) | 2002-11-14 | 2007-10-30 | siRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US11/980,300 Expired - Fee Related US7592443B2 (en) | 2002-11-14 | 2007-10-30 | siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4) |
US11/980,263 Expired - Fee Related US7632939B2 (en) | 2002-11-14 | 2007-10-30 | siRNA targeting proto-oncogene MET |
US12/321,749 Expired - Fee Related US7666853B2 (en) | 2002-11-14 | 2009-01-23 | siRNA targeting connective tissue growth factor (CTGF) |
US12/322,387 Expired - Fee Related US7589191B2 (en) | 2002-11-14 | 2009-02-02 | siRNA targeting hypoxia-inducible factor 1 |
US12/455,098 Expired - Fee Related US7741470B2 (en) | 2002-11-14 | 2009-05-28 | siRNA targeting gremlin |
US12/460,876 Abandoned US20100004141A1 (en) | 2002-11-14 | 2009-07-24 | siRNA targeting polo-like Kinase-1 (PLK-1) |
US12/462,029 Expired - Fee Related US7745612B2 (en) | 2002-11-14 | 2009-07-28 | siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4) |
US12/462,420 Expired - Fee Related US7737267B2 (en) | 2002-11-14 | 2009-08-04 | siRNA targeting hypoxia-inducible factor 1 |
US12/462,820 Expired - Fee Related US8022198B2 (en) | 2002-11-14 | 2009-08-10 | siRNA targeting histamine receptor H1 |
US12/584,352 Expired - Fee Related US8222395B2 (en) | 2002-11-14 | 2009-09-03 | siRNA targeting kinase insert domain receptor (KDR) |
US12/584,850 Expired - Fee Related US7897754B2 (en) | 2002-11-14 | 2009-09-11 | SiRNA targeting ras-related nuclear protein RAN |
US12/586,167 Expired - Fee Related US7855186B2 (en) | 2002-11-14 | 2009-09-17 | siRNA targeting TIE-2 |
US12/589,879 Expired - Fee Related US8039610B2 (en) | 2002-11-14 | 2009-10-29 | siRNA targeting superoxide dismutase 1 (SOD1) |
US12/590,097 Expired - Fee Related US7816512B2 (en) | 2002-11-14 | 2009-11-02 | siRNA targeting proto-oncogene MET |
US12/590,252 Expired - Fee Related US7829696B2 (en) | 2002-11-14 | 2009-11-04 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US12/592,872 Active 2024-09-09 US8304528B2 (en) | 2002-11-14 | 2009-12-03 | SiRNA targeting fructose-1, 6-bisphosphatase 1 (FBP1) |
US12/653,120 Expired - Fee Related US8022199B2 (en) | 2002-11-14 | 2009-12-08 | SiRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US12/653,402 Expired - Fee Related US7807820B2 (en) | 2002-11-14 | 2009-12-11 | siRNA targeting beta secretase (BACE) |
US12/655,107 Expired - Fee Related US7833989B2 (en) | 2002-11-14 | 2009-12-23 | siRNA targeting connective tissue growth factor (CTGF) |
US12/657,448 Expired - Lifetime US8067576B2 (en) | 2002-11-14 | 2010-01-21 | siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase) |
US12/660,582 Expired - Lifetime US8247169B2 (en) | 2002-11-14 | 2010-03-01 | SiRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2) |
US12/798,603 Expired - Fee Related US8030476B2 (en) | 2002-11-14 | 2010-04-07 | siRNA targeting gremlin |
US12/798,802 Expired - Fee Related US7935813B2 (en) | 2002-11-14 | 2010-04-12 | siRNA target hypoxia-inducible factor 1 |
US12/798,906 Active 2024-06-21 US8236942B2 (en) | 2002-11-14 | 2010-04-13 | SiRNA targeting glucagon receptor (GCGR) |
US12/799,758 Expired - Lifetime US8217162B2 (en) | 2002-11-14 | 2010-04-30 | siRNA targeting interleukin-1 receptor-associated kinase 4(IRAK4) |
US12/799,975 Abandoned US20100267587A1 (en) | 2002-11-14 | 2010-05-05 | siRNA targeting cyclin dependent kinase 11 (CDK11) |
US12/804,014 Expired - Fee Related US8071754B2 (en) | 2002-11-14 | 2010-07-12 | siRNA targeting apolipoprotein B (APOB) |
US12/806,570 Expired - Fee Related US7999097B2 (en) | 2002-11-14 | 2010-08-17 | siRNA targeting beta secretase (BACE) |
US12/807,526 Expired - Fee Related US8222396B2 (en) | 2002-11-14 | 2010-09-08 | SiRNA targeting proto-oncogene MET |
US12/924,078 Expired - Lifetime US8268985B2 (en) | 2002-11-14 | 2010-09-20 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US12/924,653 Expired - Lifetime US8138329B2 (en) | 2002-11-14 | 2010-10-01 | siRNA targeting connective tissue growth factor (CTGF) |
US12/927,144 Expired - Fee Related US8314229B2 (en) | 2002-11-14 | 2010-11-08 | siRNA targeting tie-2 |
US13/135,336 Expired - Lifetime US8293887B2 (en) | 2002-11-14 | 2011-07-01 | SiRNA targeting beta secretase (BACE) |
US13/136,780 Expired - Fee Related US8633306B2 (en) | 2002-11-14 | 2011-08-10 | SiRNA targeting histamine receptor H1 |
US13/136,812 Expired - Fee Related US8426579B2 (en) | 2002-11-14 | 2011-08-11 | SiRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US13/199,240 Abandoned US20110319297A1 (en) | 2002-11-14 | 2011-08-23 | siRNA targeting gremlin |
US13/199,946 Abandoned US20120015850A1 (en) | 2002-11-14 | 2011-09-13 | siRNA targeting Superoxide |
US13/317,752 Expired - Lifetime US8232386B2 (en) | 2002-11-14 | 2011-10-27 | SiRNA targeting apolipoprotein B (APOB) |
US13/385,320 Expired - Fee Related US8461326B2 (en) | 2002-11-14 | 2012-02-14 | SiRNA targeting connective tissue growth factor (CTGF) |
US13/489,725 Abandoned US20120252873A1 (en) | 2002-11-14 | 2012-06-06 | siRNA Targeting Interleukin-1 Receptor-associated Kinase 4 (IRAK4) |
US13/494,360 Abandoned US20120258888A1 (en) | 2002-11-14 | 2012-06-12 | siRNA Targeting Proto-oncogene MET |
US13/524,015 Expired - Fee Related US8575329B2 (en) | 2002-11-14 | 2012-06-15 | siRNA targeting kinase insert domain receptor (KDR) |
US13/536,005 Expired - Fee Related US8445668B2 (en) | 2002-11-14 | 2012-06-28 | SiRNA targeting apolipoprotein (APOB) |
US13/539,630 Abandoned US20120270751A1 (en) | 2002-11-14 | 2012-07-02 | siRNA Targeting Diacylglycerol O-Acyltransferase Homolog 2 (DGAT2) |
US13/542,332 Abandoned US20120283311A1 (en) | 2002-11-14 | 2012-07-05 | siRNA Targeting Glucagon Receptor (GCCR) |
US13/551,794 Expired - Fee Related US8658784B2 (en) | 2002-11-14 | 2012-07-18 | siRNA targeting amyloid beta (A4) precursor protein (APP) |
US13/613,910 Abandoned US20130023446A1 (en) | 2002-11-14 | 2012-09-13 | siRNA Targeting Beta Secretase (BACE) |
US13/632,519 Abandoned US20130059760A1 (en) | 2002-11-14 | 2012-10-01 | siRNA Targeting Fructose-1, 6-bisphosphatase 1 (FBP1) |
US13/647,869 Expired - Fee Related US8658785B1 (en) | 2002-11-14 | 2012-10-09 | siRNA targeting tie-2 |
US13/847,544 Expired - Fee Related US8883998B2 (en) | 2002-11-14 | 2013-03-20 | siRNA targeting myeloid differentiation primary response gene (88) (MYD88) |
US13/867,175 Abandoned US20130225447A1 (en) | 2002-11-14 | 2013-04-22 | siRNA Targeting Apolipoprotein B (APOB) |
US14/099,339 Expired - Fee Related US8907077B2 (en) | 2002-11-14 | 2013-12-06 | siRNA targeting TIE-2 |
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Publication number | Priority date | Publication date | Assignee | Title |
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US7618948B2 (en) | 2002-11-26 | 2009-11-17 | Medtronic, Inc. | Devices, systems and methods for improving and/or cognitive function through brain delivery of siRNA |
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US20060142228A1 (en) | 2004-12-23 | 2006-06-29 | Ambion, Inc. | Methods and compositions concerning siRNA's as mediators of RNA interference |
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US9248141B2 (en) | 2005-02-03 | 2016-02-02 | Coda Therapeutics, Inc. | Methods of treatment by administering anti-connexin proteins and mimetics |
JP4131271B2 (en) * | 2005-03-30 | 2008-08-13 | ソニー株式会社 | Information processing apparatus and method, and program |
CN101313070B (en) * | 2005-09-20 | 2015-07-15 | J.R.西姆普罗特公司 | Low acrylamide foods |
WO2007047913A2 (en) * | 2005-10-20 | 2007-04-26 | Isis Pharmaceuticals, Inc | Compositions and methods for modulation of lmna expression |
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US20070135518A1 (en) * | 2005-12-09 | 2007-06-14 | Marta Weinstock-Rosin | Use of low-dose ladostigil for neuroprotection |
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TW201336514A (en) | 2006-04-13 | 2013-09-16 | Alcon Res Ltd | RNAi-mediated inhibition of spleen tyrosine kinase-related inflammatory conditions |
EP2003971A4 (en) * | 2006-04-13 | 2010-10-27 | Alcon Res Ltd | Rnai-mediated inhibition of histamine receptor h1-related conditions |
WO2007147067A2 (en) | 2006-06-14 | 2007-12-21 | Rosetta Inpharmatics Llc | Methods and compositions for regulating cell cycle progression |
AU2007286545A1 (en) * | 2006-08-24 | 2008-02-28 | Alcon Research, Ltd. | RNAi-mediated inhibition of gremlin for treatment of IOP-related conditions |
US20080214486A1 (en) * | 2006-11-28 | 2008-09-04 | Alcon Manufacturing, Ltd. | RNAi-MEDIATED INHIBITION OF AQUAPORIN 4 FOR TREATMENT OF IOP-RELATED CONDITIONS |
WO2008075871A1 (en) * | 2006-12-18 | 2008-06-26 | Panagene Inc. | Peptide nucleic acid oligomers comprising universal bases, preparation methods thereof, and kits, devices and methods for the analysis, detection or modulation of nucleic acids using the same |
CA2679342A1 (en) * | 2007-03-02 | 2008-09-12 | Mdrna, Inc. | Nucleic acid compounds for inhibiting hif1a gene expression and uses thereof |
ES2447868T3 (en) | 2007-03-14 | 2014-03-13 | Bionsil S.R.L. In Liquidazione | BTK inhibitors for use in the treatment of epithelial tumors resistant to chemotherapeutic drugs |
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US20100184823A1 (en) | 2007-07-05 | 2010-07-22 | Mark Aron Labow | dsRNA For Treating Viral Infection |
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AU2008343754A1 (en) * | 2007-12-21 | 2009-07-09 | Coda Therapeutics, Inc. | Use of anti-connexin polynucleotides for the treatment of surgical adhesions |
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WO2009102931A1 (en) * | 2008-02-15 | 2009-08-20 | Alcon Research, Ltd. | Rnai-mediated inhibition of connexin 43 for treatment of iop-related conditions |
WO2009113579A1 (en) * | 2008-03-11 | 2009-09-17 | 学校法人埼玉医科大学 | Double-stranded nucleic acid molecule suitable for prevention or treatment of cancer, cancer cell proliferation inhibitor, and pharmaceutical preparation |
DK2279254T3 (en) | 2008-04-15 | 2017-09-18 | Protiva Biotherapeutics Inc | PRESENT UNKNOWN LIPID FORMS FOR NUCLEIC ACID ADMINISTRATION |
KR101707669B1 (en) * | 2008-04-17 | 2017-02-16 | 더 존스 홉킨스 유니버시티 | On01910. na enhances chemotherapeutic agent activity in drug-resistant cancers |
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WO2009158432A2 (en) | 2008-06-27 | 2009-12-30 | Amgen Inc. | Ang-2 inhibition to treat multiple sclerosis |
EP3081648A1 (en) | 2008-08-25 | 2016-10-19 | Excaliard Pharmaceuticals, Inc. | Antisense oligonucleotides directed against connective tissue growth factor and uses thereof |
US8946172B2 (en) * | 2008-08-25 | 2015-02-03 | Excaliard Pharmaceuticals, Inc. | Method for reducing scarring during wound healing using antisense compounds directed to CTGF |
WO2010027279A2 (en) * | 2008-09-04 | 2010-03-11 | Genesis Research And Development Corporation Limited | Compositions and methods for the treatment and prevention of neoplastic disorders |
US10138485B2 (en) | 2008-09-22 | 2018-11-27 | Rxi Pharmaceuticals Corporation | Neutral nanotransporters |
EP3025727A1 (en) | 2008-10-02 | 2016-06-01 | The J. David Gladstone Institutes | Methods of treating liver disease |
US9095592B2 (en) * | 2008-11-07 | 2015-08-04 | The Research Foundation For The State University Of New York | Bruton's tyrosine kinase as anti-cancer drug target |
US20100233270A1 (en) | 2009-01-08 | 2010-09-16 | Northwestern University | Delivery of Oligonucleotide-Functionalized Nanoparticles |
WO2010107952A2 (en) * | 2009-03-19 | 2010-09-23 | Merck Sharp & Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF CONNECTIVE TISSUE GROWTH FACTOR (CTGF) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
US20110045080A1 (en) * | 2009-03-24 | 2011-02-24 | William Marsh Rice University | Single-Walled Carbon Nanotube/Bioactive Substance Complexes and Methods Related Thereto |
EA201171175A1 (en) * | 2009-03-27 | 2012-05-30 | Мерк Шарп Энд Домэ Корп. | PHK-MEDIATED INTERFERENCE INHIBITING EXPRESSION OF A CELL INTERCEPTIVE ADHESION MOLECULE 1 (ICAM-1) WITH USING A SHORT INTERFERATING NUCLEINIC ACID (IC) -1 |
EP2414524B1 (en) * | 2009-04-03 | 2017-08-23 | Centre National De La Recherche Scientifique | Gene transfer vectors comprising genetic insulator elements and methods to identify genetic insulator elements |
US20110038852A1 (en) * | 2009-06-10 | 2011-02-17 | 3-V Biosciences, Inc. | Antivirals that target transporters, carriers, and ion channels |
CN101851625B (en) * | 2009-06-25 | 2012-07-18 | 上海交通大学 | RNA interference sequences of glucagon receptor gene |
CN101586103B (en) * | 2009-06-25 | 2010-12-08 | 上海交通大学 | RNA interference sequence of glucagon receptor gene |
CN101851624B (en) * | 2009-06-25 | 2012-04-18 | 上海交通大学 | RNA interference sequences of glucagon receptor gene |
JP5766188B2 (en) | 2009-07-01 | 2015-08-19 | プロチバ バイオセラピューティクス インコーポレイティッド | Lipid formulations for delivering therapeutic agents to solid tumors |
US10165286B2 (en) | 2009-07-08 | 2018-12-25 | Dejero Labs Inc. | System and method for automatic encoder adjustment based on transport data |
US9222086B2 (en) * | 2009-09-23 | 2015-12-29 | Protiva Biotherapeutics, Inc. | Compositions and methods for silencing genes expressed in cancer |
US9799416B2 (en) * | 2009-11-06 | 2017-10-24 | Terrapower, Llc | Methods and systems for migrating fuel assemblies in a nuclear fission reactor |
US20110152343A1 (en) * | 2009-12-22 | 2011-06-23 | Functional Genetics, Inc. | Protease inhibitors and broad-spectrum antiviral |
US20130023578A1 (en) * | 2009-12-31 | 2013-01-24 | Samyang Biopharmaceuticals Corporation | siRNA for inhibition of c-Met expression and anticancer composition containing the same |
US8653047B2 (en) | 2010-01-08 | 2014-02-18 | Isis Pharmaceuticals, Inc. | Modulation of angiopoietin-like 3 expression |
CN102102101A (en) * | 2010-03-05 | 2011-06-22 | 重庆医科大学 | SiRNA for inhibiting expression of Plk1 and use thereof |
FR2957426B1 (en) * | 2010-03-12 | 2012-03-02 | Thales Sa | AUTONOMOUS PSEUDOLITE POSITIONING SYSTEM IN THE CONSTRAINED ZONE AND METHOD OF IMPLEMENTING THE SAME |
US9095504B2 (en) | 2010-03-24 | 2015-08-04 | Rxi Pharmaceuticals Corporation | RNA interference in ocular indications |
JP6060071B2 (en) * | 2010-03-24 | 2017-01-11 | アールエックスアイ ファーマシューティカルズ コーポレーション | RNA interference in skin and fibrosis applications |
EP2380595A1 (en) | 2010-04-19 | 2011-10-26 | Nlife Therapeutics S.L. | Compositions and methods for selective delivery of oligonucleotide molecules to specific neuron types |
WO2011133658A1 (en) | 2010-04-22 | 2011-10-27 | Boston Medical Center Corporation | Compositions and methods for targeting and delivering therapeutics into cells |
EP2575799A4 (en) * | 2010-05-19 | 2013-11-13 | Beth Israel Hospital | Methods for treating inflammatory autoimmune disorders |
EP2390327A1 (en) | 2010-05-27 | 2011-11-30 | Sylentis S.A. | siRNA and their use in methods and compositions for the treatment and/or prevention of eye conditions |
US9316346B2 (en) | 2010-06-09 | 2016-04-19 | Colebrook Bosson Saunders (Products) Limited | Support system |
US9074721B2 (en) | 2010-06-09 | 2015-07-07 | Alex Lau | Support system |
CA2803882C (en) * | 2010-06-23 | 2022-10-18 | Opko Curna, Llc | Treatment of sodium channel, voltage-gated, alpha subunit (scna) related diseases by inhibition of natural antisense transcript to scna |
CA2804847A1 (en) | 2010-07-28 | 2012-02-02 | Alcon Research Ltd. | Sirna targeting vegfa and methods for treatment in vivo |
USD684982S1 (en) | 2010-08-11 | 2013-06-25 | Colebrook Bosson Saunders (Products) Limited | Display support with indicator window |
WO2012027713A2 (en) * | 2010-08-26 | 2012-03-01 | Alnylam Pharmaceuticals, Inc. | Compositions and methods for inhibition of snca |
WO2012034942A1 (en) | 2010-09-13 | 2012-03-22 | Santaris Pharma A/S | Compounds for the modulation of aurora kinase b expression |
ES2732929T3 (en) | 2010-10-22 | 2019-11-26 | Olix Pharmaceuticals Inc | Nucleic acid molecules that induce RNA interference and uses thereof |
WO2012068073A2 (en) | 2010-11-15 | 2012-05-24 | University Of Florida Research Foundation, Inc. | Therapeutic and diagnostic applications targeting tnk-1 |
EP2640853B1 (en) | 2010-11-17 | 2018-12-26 | Ionis Pharmaceuticals, Inc. | Modulation of alpha synuclein expression |
US20150247148A1 (en) * | 2011-01-31 | 2015-09-03 | Kyowa Hakko Kirin Co., Ltd. | Composition for suppressing expression of target gene |
SI2670411T1 (en) | 2011-02-02 | 2019-06-28 | Excaliard Pharmaceuticals, Inc. | Antisense compounds targeting connective tissue growth factor (ctgf) for use in a method of treating keloids or hypertrophic scars |
CN103547588B (en) | 2011-04-13 | 2016-06-29 | Isis制药公司 | The antisense that PTP1B expresses regulates |
CA2831572C (en) | 2011-05-02 | 2019-11-26 | Immunomedics, Inc. | Ultrafiltration concentration of allotype selected antibodies for small-volume administration |
US8802839B2 (en) | 2011-07-15 | 2014-08-12 | Fibrogen, Inc. | Connective tissue growth factor antisense oligonucleotides |
WO2013012806A2 (en) | 2011-07-18 | 2013-01-24 | University Of Kentucky Research Foundation | Protection of cells from alu-rna-induced degenereation and inhibitors for protecting cells |
CN104024413B (en) | 2011-10-19 | 2016-08-17 | 苏州瑞博生物技术有限公司 | SiRNA and application thereof and the method for suppression plk1 gene expression |
WO2013068836A1 (en) | 2011-11-07 | 2013-05-16 | INSERM (Institut National de la Santé et de la Recherche Médicale) | A ddr1 antagonist or an inhibitor of ddr1 gene expression for use in the prevention or treatment of crescentic glomerulonephritis |
EP2725103A3 (en) * | 2011-11-14 | 2016-01-06 | Silenseed Ltd | Methods and compositions for treating prostate cancer |
US9617331B2 (en) | 2011-11-27 | 2017-04-11 | Yeda Research And Development Co. Ltd. | Methods of regulating angiogenesis by administering agents which increase apoB-100 polypeptide |
US9707235B1 (en) | 2012-01-13 | 2017-07-18 | University Of Kentucky Research Foundation | Protection of cells from degeneration and treatment of geographic atrophy |
WO2013123996A1 (en) * | 2012-02-24 | 2013-08-29 | Astrazeneca Uk Limited | Novel sirna inhibitors of human icam-1 |
DK2817287T3 (en) | 2012-02-24 | 2019-01-02 | Arbutus Biopharma Corp | TRIALKYL CATIONIC LIPID AND METHODS FOR USING IT |
AU2013225619A1 (en) * | 2012-02-29 | 2014-10-16 | Benitec Biopharma Limited | Pain treatment |
AU2013232855B2 (en) | 2012-03-15 | 2016-10-27 | Hyun Kee Kim | Gremlin-1 antibody |
DE202012003753U1 (en) * | 2012-04-13 | 2013-07-17 | Joseph Vögele AG | Cross distribution arrangement for a paver |
CN102690826B (en) * | 2012-04-19 | 2014-03-05 | 山西医科大学 | shRNA for specifically reducing human Aurora-A gene expression and application thereof |
CN108148838A (en) | 2012-05-22 | 2018-06-12 | 奥利克斯医药有限公司 | The nucleic acid molecules and purposes of induction RNA interference with intracellular penetration capacity |
US9353422B2 (en) * | 2012-06-04 | 2016-05-31 | Academia Sinica | USP37 inactivation as a treatment for PLZF/RARA-associated acute promyelocytic leukemia |
CA2874864C (en) | 2012-08-14 | 2023-02-21 | Ibc Pharmaceuticals, Inc. | T-cell redirecting bispecific antibodies for treatment of disease |
ES2750125T3 (en) | 2012-09-05 | 2020-03-25 | Sylentis Sau | SiRNA and its use in methods and compositions for the treatment and / or prevention of eye conditions |
GB201215857D0 (en) | 2012-09-05 | 2012-10-24 | Sylentis Sau | siRNA and their use in methods and compositions for the treatment and/or prevention of eye conditions |
AU2013336581A1 (en) | 2012-10-26 | 2015-06-11 | Nlife Therapeutics, S.L. | Compositions and methods for the treatment of Parkinson disease by the selective delivery of oligonucleotide molecules to specific neuron types |
EP2914260A1 (en) | 2012-10-31 | 2015-09-09 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for preventing antiphospholipid syndrome (aps) |
EP2943579B1 (en) | 2013-01-10 | 2018-09-12 | Dharmacon, Inc. | Libraries and methods for generating molecules |
EP2948550A1 (en) * | 2013-01-28 | 2015-12-02 | Council of Scientific & Industrial Research | METHOD FOR INHIBITING TUMOR GROWTH THROUGH RNA-INTERFERENCE USING LIPOSOMALLY ASSOCIATED CDC20 siRNA |
WO2014144423A2 (en) | 2013-03-15 | 2014-09-18 | Techulon Inc. | Antisense molecules for treatment of staphylococcus aureus infection |
KR102206573B1 (en) | 2013-03-15 | 2021-01-25 | 테출론 인코포레이티드 | Antisense molecules for treatment of staphylococcus aureus infection |
RS57418B1 (en) | 2013-05-22 | 2018-09-28 | Alnylam Pharmaceuticals Inc | Serpina1 irna compositions and methods of use thereof |
EP3444350B1 (en) | 2013-07-03 | 2021-12-01 | Dicerna Pharmaceuticals, Inc. | Methods and compositions for the specific inhibition of alpha-1 antitrypsin by double-stranded rna |
CN110592083A (en) * | 2013-07-05 | 2019-12-20 | 柏业公司 | Respiratory disease related gene specific siRNA, double helix oligo RNA structure containing siRNA and application thereof |
KR20150006742A (en) * | 2013-07-09 | 2015-01-19 | (주)바이오니아 | Liver cancer related genes-specific siRNA, double-stranded oligo RNA molecules comprising the siRNA, and composition for the prevention or treatment of cancer comprising the same |
KR102365486B1 (en) | 2013-08-28 | 2022-02-18 | 아이오니스 파마수티컬즈, 인코포레이티드 | Modulation of prekallikrein (pkk) expression |
EP3077511A4 (en) | 2013-12-06 | 2017-07-05 | Dicerna Pharmaceuticals Inc. | Methods and compositions for the specific inhibition of transthyretin (ttr) by double-stranded rna |
IL282401B (en) * | 2013-12-12 | 2022-08-01 | Alnylam Pharmaceuticals Inc | Complement component irna compositions and methods of use thereof |
NZ720286A (en) | 2013-12-24 | 2022-12-23 | Ionis Pharmaceuticals Inc | Modulation of angiopoietin-like 3 expression |
AU2014369850B2 (en) | 2013-12-27 | 2021-04-08 | Dicerna Pharmaceuticals, Inc. | Methods and compositions for the specific inhibition of Glycolate Oxidase (HAO1) by double-stranded RNA |
US9566280B2 (en) | 2014-01-28 | 2017-02-14 | Massachusetts Institute Of Technology | Combination therapies and methods of use thereof for treating cancer |
MX2016010683A (en) | 2014-02-21 | 2017-05-11 | Ibc Pharmaceuticals Inc | Disease therapy by inducing immune response to trop-2 expressing cells. |
US9139649B2 (en) | 2014-02-25 | 2015-09-22 | Immunomedics, Inc. | Humanized anti-CD22 antibody |
WO2015132303A1 (en) | 2014-03-04 | 2015-09-11 | Sylentis Sau | Sirnas and their use in methods and compositions for the treatment and/or prevention of eye conditions |
RU2704619C2 (en) | 2014-04-01 | 2019-10-30 | Биоген Ма Инк. | Compositions for modulating expression of sod-1 |
KR20150137473A (en) * | 2014-05-29 | 2015-12-09 | 한국과학기술연구원 | siRNA for Inhibition of USP15 Expression and Pharmaceutical Composition Containing the same |
AU2015286663B2 (en) * | 2014-07-10 | 2021-09-23 | Stichting Radboud Universitair Medisch Centrum | Antisense oligonucleotides for the treatment of usher syndrome type 2 |
ES2851925T3 (en) * | 2014-07-29 | 2021-09-09 | Wellmarker Bio Co Ltd | MET and IGSF1 inhibitors for the treatment of cancer |
EA201790434A1 (en) | 2014-08-22 | 2017-07-31 | Окленд Юнисервисиз Лимитед | CHANNEL MODULATORS |
CN104258377B (en) * | 2014-09-10 | 2017-06-30 | 中南大学湘雅医院 | Application of the PIK3C2A albumen in liver-cancer medicine is treated |
CN107257858B (en) | 2014-10-10 | 2022-03-08 | 戴瑟纳制药公司 | Therapeutic inhibition of lactate dehydrogenase and reagents therefor |
EP3212794B1 (en) | 2014-10-30 | 2021-04-07 | Genzyme Corporation | Polynucleotide agents targeting serpinc1 (at3) and methods of use thereof |
SG11201703281RA (en) | 2014-11-14 | 2017-05-30 | Voyager Therapeutics Inc | Compositions and methods of treating amyotrophic lateral sclerosis (als) |
EP3907287A1 (en) | 2014-11-14 | 2021-11-10 | Voyager Therapeutics, Inc. | Modulatory polynucleotides |
CN107106493A (en) | 2014-11-21 | 2017-08-29 | 西北大学 | The sequence-specific cellular uptake of spherical nucleic acid nano particle conjugate |
CN104450710B (en) * | 2014-11-28 | 2018-06-05 | 广州市锐博生物科技有限公司 | Inhibit nucleic acid oligomer and its application of MYD88 genes |
US10036017B2 (en) | 2015-02-17 | 2018-07-31 | Dicerna Pharmaceuticals, Inc. | Methods and compositions for the specific inhibition of complement component 5(C5) by double-stranded RNA |
US11129844B2 (en) | 2015-03-03 | 2021-09-28 | Ionis Pharmaceuticals, Inc. | Compositions and methods for modulating MECP2 expression |
CN104673799B (en) * | 2015-03-09 | 2019-02-05 | 浙江大学 | It targets the siRNA of people RAN and its is preparing the purposes in anti-viral hepatitis type C drug |
WO2016183009A2 (en) | 2015-05-08 | 2016-11-17 | Dicerna Pharmaceuticals, Inc. | Methods and compositions for the specific inhibition of antithrombin 3 (at3) by double-stranded rna |
WO2017053781A1 (en) | 2015-09-25 | 2017-03-30 | Ionis Pharmaceuticals, Inc. | Compositions and methods for modulating ataxin 3 expression |
KR20180071362A (en) | 2015-11-16 | 2018-06-27 | 올릭스 주식회사 | Treatment of age-related macular degeneration with RNA complex targeting MyD88 or TLR3 |
CN108601795A (en) | 2015-12-07 | 2018-09-28 | 建新公司 | Method and composition for treating SERPINC1 associated diseases |
EP3386544B1 (en) * | 2015-12-10 | 2020-11-25 | Fibrogen, Inc. | Methods for treatment of motor neuron diseases |
CN105462978B (en) * | 2015-12-18 | 2018-05-15 | 中国科学院北京基因组研究所 | One species specificity suppresses siRNA and its application of MAGEA1 gene expressions |
CN105462977B (en) * | 2015-12-18 | 2018-05-11 | 中国科学院北京基因组研究所 | One species specificity suppresses siRNA and its application of MAGEA1 gene expressions |
WO2017132669A1 (en) | 2016-01-31 | 2017-08-03 | University Of Massachusetts | Branched oligonucleotides |
KR20180104692A (en) | 2016-02-02 | 2018-09-21 | 올릭스 주식회사 | Treatment of angiogenesis related diseases using RNA complexes targeting ANGPT2 and PDGFB |
EP3411480A4 (en) | 2016-02-02 | 2020-01-22 | Olix Pharmaceuticals, Inc. | TREATMENT OF ATOPIC DERMATITIS AND ASTHMA USING RNA COMPLEXES THAT TARGET lL4R , TRPA1, OR F2RL1 |
CA3020487C (en) | 2016-04-11 | 2022-05-31 | Olix Pharmaceuticals, Inc. | Treatment of idiopathic pulmonary fibrosis using rna complexes that target connective tissue growth factor |
IL262784B2 (en) | 2016-05-18 | 2023-10-01 | Voyager Therapeutics Inc | Modulatory polynucleotides |
CN109312403B (en) * | 2016-06-17 | 2023-06-27 | 豪夫迈·罗氏有限公司 | In vitro nephrotoxicity screening assay |
KR101916652B1 (en) | 2016-06-29 | 2018-11-08 | 올릭스 주식회사 | Compounds improving RNA interference of small interfering RNA and use thereof |
US11364304B2 (en) | 2016-08-25 | 2022-06-21 | Northwestern University | Crosslinked micellar spherical nucleic acids |
JOP20190104A1 (en) | 2016-11-10 | 2019-05-07 | Ionis Pharmaceuticals Inc | Compounds and methods for reducing atxn3 expression |
TWI788312B (en) | 2016-11-23 | 2023-01-01 | 美商阿尼拉製藥公司 | SERPINA1 iRNA COMPOSITIONS AND METHODS OF USE THEREOF |
EP3551047A1 (en) | 2016-12-07 | 2019-10-16 | Progenity, Inc. | Gastrointestinal tract detection methods, devices and systems |
BR112019011689A2 (en) | 2016-12-14 | 2019-10-22 | Progenity Inc | treating a gastrointestinal tract disease with a tnf inhibitor |
EP3580339A4 (en) | 2017-02-10 | 2020-12-23 | Research & Business Foundation Sungkyunkwan University | Long double-stranded rna for rna interference |
DE102017206836A1 (en) * | 2017-04-24 | 2018-10-25 | Zf Friedrichshafen Ag | Transmission for a motor vehicle |
WO2018199777A1 (en) | 2017-04-28 | 2018-11-01 | Auckland Uniservices Limited | Methods of treatment and novel constructs |
CN110913866A (en) | 2017-05-05 | 2020-03-24 | 沃雅戈治疗公司 | Compositions and methods for treating Amyotrophic Lateral Sclerosis (ALS) |
WO2018209270A1 (en) | 2017-05-11 | 2018-11-15 | Northwestern University | Adoptive cell therapy using spherical nucleic acids (snas) |
PE20201287A1 (en) | 2017-09-14 | 2020-11-24 | Arrowhead Pharmaceuticals Inc | IRNA AGENTS AND COMPOSITIONS TO INHIBIT THE EXPRESSION OF ANGIOPOYETIN TYPE 3 (ANGPTL3) AND METHODS OF USE |
EP3691670A4 (en) * | 2017-10-02 | 2021-08-04 | Cedars-Sinai Medical Center | Methods and compositions for efficient delivery through multiple bio barriers |
EP3679141B1 (en) | 2017-10-13 | 2023-06-07 | Novo Nordisk Health Care AG | Methods and compositions for inhibiting expression of ldha |
US20200237799A1 (en) | 2017-10-16 | 2020-07-30 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis (als) |
CN111479924A (en) | 2017-10-16 | 2020-07-31 | 沃雅戈治疗公司 | Treatment of amyotrophic lateral sclerosis (A L S) |
TWI809004B (en) | 2017-11-09 | 2023-07-21 | 美商Ionis製藥公司 | Compounds and methods for reducing snca expression |
JP7365052B2 (en) | 2017-12-01 | 2023-10-19 | スーチョウ リボ ライフ サイエンス カンパニー、リミテッド | Nucleic acids, compositions and complexes containing the nucleic acids, and preparation methods and uses |
JP2021504415A (en) | 2017-12-01 | 2021-02-15 | スーチョウ リボ ライフ サイエンス カンパニー、リミテッドSuzhou Ribo Life Science Co., Ltd. | Compositions and complexes containing double-stranded oligonucleotides, double-stranded oligonucleotides, and preparation methods and uses. |
KR102443358B1 (en) | 2017-12-06 | 2022-09-14 | 어비디티 바이오사이언시스 인크. | Compositions and methods for treating muscular dystrophy and myotonic dystrophy |
BR112020012523A2 (en) | 2017-12-21 | 2020-11-24 | F. Hoffmann-La Roche Ag | complementary diagnosis for htra1 rna antagonists |
AU2018394875B2 (en) | 2017-12-29 | 2023-08-03 | Suzhou Ribo Life Science Co., Ltd. | Conjugates and preparation and use thereof |
WO2019136459A1 (en) | 2018-01-08 | 2019-07-11 | Iovance Biotherapeutics, Inc. | Processes for generating til products enriched for tumor antigen-specific t-cells |
SG11202006541UA (en) | 2018-01-08 | 2020-08-28 | Iovance Biotherapeutics Inc | Processes for generating til products enriched for tumor antigen-specific t-cells |
US11713446B2 (en) | 2018-01-08 | 2023-08-01 | Iovance Biotherapeutics, Inc. | Processes for generating TIL products enriched for tumor antigen-specific T-cells |
CN112424353A (en) | 2018-01-12 | 2021-02-26 | 罗氏创新中心哥本哈根有限公司 | Alpha-synuclein antisense oligonucleotides and uses thereof |
US11732260B2 (en) | 2018-03-02 | 2023-08-22 | Ionis Pharmaceuticals, Inc. | Compounds and methods for the modulation of amyloid-β precursor protein |
CR20200604A (en) | 2018-05-09 | 2021-02-09 | Ionis Pharmaceuticals Inc | Compounds and methods for reducing atxn3 expression |
US11162083B2 (en) | 2018-06-14 | 2021-11-02 | University Of South Carolina | Peptide based inhibitors of Raf kinase protein dimerization and kinase activity |
WO2019246313A1 (en) | 2018-06-20 | 2019-12-26 | Progenity, Inc. | Treatment of a disease of the gastrointestinal tract with a tnf inhibitor |
US11332746B1 (en) | 2018-06-27 | 2022-05-17 | Ionis Pharmaceuticals, Inc. | Compounds and methods for reducing LRRK2 expression |
AU2019299861A1 (en) | 2018-07-02 | 2021-01-14 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis and disorders associated with the spinal cord |
JP2021533800A (en) | 2018-08-21 | 2021-12-09 | スーチョウ リボ ライフ サイエンス カンパニー、リミテッドSuzhou Ribo Life Science Co., Ltd. | Nucleic acid, drug compositions and complexes containing the nucleic acid, and their use |
CN111655297A (en) | 2018-09-30 | 2020-09-11 | 苏州瑞博生物技术有限公司 | siRNA conjugate and preparation method and application thereof |
WO2020106750A1 (en) | 2018-11-19 | 2020-05-28 | Progenity, Inc. | Methods and devices for treating a disease with biotherapeutics |
CA3119234A1 (en) * | 2018-11-23 | 2020-05-28 | Silence Therapeutics Gmbh | Nucleic acids for inhibiting expression of c3 in a cell |
EP3956450A4 (en) | 2019-07-26 | 2022-11-16 | Ionis Pharmaceuticals, Inc. | Compounds and methods for modulating gfap |
KR20220047865A (en) | 2019-08-27 | 2022-04-19 | 사일런스 테라퓨틱스 게엠베하 | Nucleic Acids for Inhibiting Expression of C3 in Cells |
CN112442500A (en) * | 2019-08-30 | 2021-03-05 | 恩智(广州)医药科技有限公司 | siRNA for inhibiting MCM7, composition and application thereof |
EP4021448A4 (en) * | 2019-08-30 | 2023-12-20 | Agency for Science, Technology and Research | A method of promoting survival and/or function of a motor neuron and related agents, uses and methods |
CN110938691B (en) * | 2019-12-03 | 2023-07-07 | 兰州大学 | Application of human DUS4L gene and related products |
WO2021119482A1 (en) | 2019-12-13 | 2021-06-17 | Progenity, Inc. | Ingestible device for delivery of therapeutic agent to the gastrointestinal tract |
KR20220156867A (en) | 2020-03-19 | 2022-11-28 | 어비디티 바이오사이언시스 인크. | Compositions and methods for the treatment of facial scapular brachial muscular dystrophy |
KR20220161378A (en) | 2020-03-27 | 2022-12-06 | 어비디티 바이오사이언시스 인크. | Compositions and methods for the treatment of muscular dystrophy |
CN113444723A (en) * | 2020-03-27 | 2021-09-28 | 北京键凯科技股份有限公司 | Interfering RNA for inhibiting vascular endothelial growth factor receptor 2 gene expression and application thereof |
IL297050A (en) | 2020-04-04 | 2022-12-01 | Pfizer | Methods of treating coronavirus disease 2019 |
WO2021241802A1 (en) * | 2020-05-26 | 2021-12-02 | 올릭스 주식회사 | Rnai agent targeting connective tissue growth factor and use thereof |
US11459567B2 (en) | 2020-06-24 | 2022-10-04 | Patricia Virginia Elizalde | Specific siRNA molecules, composition and use thereof for the treatment of triple negative breast cancer |
JP2023532518A (en) | 2020-06-29 | 2023-07-28 | アイオーニス ファーマシューティカルズ, インコーポレーテッド | Compounds and methods for modulating PLP1 |
CN111849992A (en) * | 2020-08-17 | 2020-10-30 | 南通大学 | siRNA molecule of targeted c-Met gene and application thereof |
UY39417A (en) | 2020-09-11 | 2022-03-31 | Arrowhead Pharmaceuticals Inc | ARNI AGENTS FOR INHIBITING DUX4 EXPRESSION, COMPOSITIONS OF SUCH AGENTS, AND METHODS OF USE |
EP4237561A1 (en) * | 2020-12-23 | 2023-09-06 | Argonaute Rna Limited | Treatment of cardiovascular disease |
TW202305131A (en) | 2021-02-12 | 2023-02-01 | 美商艾拉倫製藥股份有限公司 | SUPEROXIDE DISMUTASE 1 (SOD1) iRNA COMPOSITIONS AND METHODS OF USE THEREOF FOR TREATING OR PREVENTING SUPEROXIDE DISMUTASE 1- (SOD1-) ASSOCIATED NEURODEGENERATIVE DISEASES |
AU2022261359A1 (en) | 2021-04-19 | 2023-10-12 | Dicerna Pharmaceuticals, Inc. | Compositions and methods for inhibiting nuclear receptor subfamily 1 group h member 3 (nr1h3) expression |
WO2022248665A1 (en) | 2021-05-28 | 2022-12-01 | Novo Nordisk A/S | Compositions and methods for inhibiting mitochondria amidoxime reducing component 1 (marc1) expression |
WO2023034870A2 (en) | 2021-09-01 | 2023-03-09 | Ionis Pharmaceuticals, Inc. | Compounds and methods for reducing dmpk expression |
WO2023122805A1 (en) | 2021-12-20 | 2023-06-29 | Vestaron Corporation | Sorbitol driven selection pressure method |
WO2023133639A1 (en) * | 2022-01-13 | 2023-07-20 | University Health Network | Targeting the ythdf1 - arhgef2 axis for cancer treatment |
WO2023144798A1 (en) | 2022-01-31 | 2023-08-03 | Genevant Sciences Gmbh | Ionizable cationic lipids for lipid nanoparticles |
US20230263783A1 (en) | 2022-02-18 | 2023-08-24 | Massachusetts Institute Of Technology | Cancer treatment by combined inhibition of polo-like kinase and microtubule polymerization |
US11912997B2 (en) | 2022-06-15 | 2024-02-27 | Arrowhead Pharmaceuticals, Inc. | RNAi agents for inhibiting expression of Superoxide Dismutase 1 (SOD1), compositions thereof, and methods of use |
Citations (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998000532A2 (en) | 1996-07-01 | 1998-01-08 | Wright Jim A | Oligonucleotides from the untranslated regions of ribonucleotide reductase and their use to modulate cell growth |
US6001992A (en) | 1999-01-07 | 1999-12-14 | Isis Pharmaceuticals Inc. | Antisense modulation of novel anti-apoptotic bcl-2-related proteins |
WO2000020645A1 (en) | 1998-10-05 | 2000-04-13 | Isis Pharmaceuticals, Inc. | ANTISENSE OLIGONUCLEOTIDE MODULATION OF TUMOR NECROSIS FACTOR-α (TNF-α) EXPRESSION |
WO2000021559A2 (en) | 1998-10-09 | 2000-04-20 | Musc Foundation For Research Development | Blocking factor b to treat complement-mediated immune disease |
US6111086A (en) | 1998-02-27 | 2000-08-29 | Scaringe; Stephen A. | Orthoester protecting groups |
WO2000076497A1 (en) | 1999-06-14 | 2000-12-21 | Cancer Research Ventures Limited | Cancer therapy |
US6172216B1 (en) | 1998-10-07 | 2001-01-09 | Isis Pharmaceuticals Inc. | Antisense modulation of BCL-X expression |
US6291642B1 (en) | 1994-02-14 | 2001-09-18 | Amgen Inc. | Mammalian cell cycle protein |
WO2002044321A2 (en) | 2000-12-01 | 2002-06-06 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Rna interference mediating small rna molecules |
US20020081578A1 (en) | 1995-12-18 | 2002-06-27 | Gregory Plowman | Diagnosis and treatment of aur1 and/or aur2 related disorders |
US20020086321A1 (en) | 1993-02-02 | 2002-07-04 | Craig Ruth W. | Myeloid cell leukemia associated gene MCL-1 |
US20020086356A1 (en) | 2000-03-30 | 2002-07-04 | Whitehead Institute For Biomedical Research | RNA sequence-specific mediators of RNA interference |
US20020150945A1 (en) | 2000-12-28 | 2002-10-17 | Cell Therapeutics, Inc. | Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis |
US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
WO2003035870A1 (en) | 2001-10-26 | 2003-05-01 | Ribopharma Ag | Drug for treating a carcinoma of the pancreas |
WO2003035869A1 (en) | 2001-10-26 | 2003-05-01 | Ribopharma Ag | Use of a double-stranded ribonucleic acid for specifically inhibiting the expression of a given target gene |
US20030087259A1 (en) | 2001-04-18 | 2003-05-08 | Clancy Brian M. | Methods and compositions for regulating bone and cartilage formation |
US6566127B1 (en) | 1995-10-26 | 2003-05-20 | Ribozyme Pharmaceuticals, Inc. | Method and reagent for the treatment of diseases or conditions related to levels of vascular endothelial growth factor receptor |
US20030105051A1 (en) | 2001-05-29 | 2003-06-05 | Mcswiggen James | Nucleic acid treatment of diseases or conditions related to levels of HER2 |
US20030143732A1 (en) | 2001-04-05 | 2003-07-31 | Kathy Fosnaugh | RNA interference mediated inhibition of adenosine A1 receptor (ADORA1) gene expression using short interfering RNA |
US20030157030A1 (en) | 2001-11-02 | 2003-08-21 | Insert Therapeutics, Inc. | Methods and compositions for therapeutic use of rna interference |
WO2003070969A2 (en) | 2002-02-20 | 2003-08-28 | Sirna Therapeutics, Inc. | RNA INTERFERENCE MEDIATED INHIBITION OF BCL2 GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
WO2003070897A2 (en) | 2002-02-20 | 2003-08-28 | Ribozyme Pharmaceuticals, Incorporated | RNA INTERFERENCE MEDIATED INHIBITION OF TNF AND TNF RECEPTOR SUPERFAMILY GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
WO2003072704A2 (en) | 2002-02-20 | 2003-09-04 | Sirna Therapeutics, Inc. | RNA INTERFERENCE MEDIATED INHIBITION OF PLATELET DERIVED GROWTH FACTOR (PDGF) AND PLATELET DERIVED GROWTH FACTOR RECEPTOR (PDGFR) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
US20030228597A1 (en) | 1998-04-13 | 2003-12-11 | Cowsert Lex M. | Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation |
US20040029275A1 (en) | 2002-08-10 | 2004-02-12 | David Brown | Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs |
US20040054155A1 (en) | 2002-02-01 | 2004-03-18 | Sequitur, Inc. | Oligonucleotide compositions with enhanced efficiency |
US20040063654A1 (en) | 2001-11-02 | 2004-04-01 | Davis Mark E. | Methods and compositions for therapeutic use of RNA interference |
WO2004031237A1 (en) | 2002-09-30 | 2004-04-15 | Oncotherapy Science, Inc. | Genes and polypeptides relating to human myeloid leukemia |
US20040077574A1 (en) | 2002-05-23 | 2004-04-22 | Ceptyr, Inc. | Modulation of biological signal transduction by RNA interference |
US20040101857A1 (en) | 2002-11-23 | 2004-05-27 | Isis Pharmaceuticals Inc. | Modulation of cytokine-inducible kinase expression |
WO2004046324A2 (en) | 2002-11-15 | 2004-06-03 | University Of Massachusetts | Allele-targeted rna interference |
WO2004048511A2 (en) | 2002-11-26 | 2004-06-10 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel viral regulatory genes and uses thereof |
US20040180357A1 (en) | 2002-11-01 | 2004-09-16 | The Trustees Of The University Of Pennsylvania | Compositions and methods for siRNA inhibition of HIF-1 alpha |
US20040192629A1 (en) | 2002-11-04 | 2004-09-30 | University Of Massachusetts | Allele-specific RNA interference |
US20040204380A1 (en) | 1999-01-07 | 2004-10-14 | Ackermann Elizabeth J | Antisense modulation of novel anti-apoptotic bcl-2-related proteins |
WO2004090105A2 (en) | 2003-04-02 | 2004-10-21 | Dharmacon, Inc. | Modified polynucleotides for use in rna interference |
US20040219671A1 (en) | 2002-02-20 | 2004-11-04 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of parkinson disease using short interfering nucleic acid (siNA) |
US20040248299A1 (en) | 2002-12-27 | 2004-12-09 | Sumedha Jayasena | RNA interference |
US20040248296A1 (en) * | 2002-03-20 | 2004-12-09 | Beresford Paul J. | HIV therapeutic |
WO2005001043A2 (en) | 2003-06-02 | 2005-01-06 | University Of Massachusetts | METHODS AND COMPOSITIONS FOR ENHANCING THE EFFICACY AND SPECIFICITY OF FNAi |
US20050048529A1 (en) | 2002-02-20 | 2005-03-03 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA) |
US20050107328A1 (en) | 2002-07-30 | 2005-05-19 | Jacqueline Wyatt | Antisense modulation of polo-like kinase expression |
US20050130181A1 (en) | 2001-05-18 | 2005-06-16 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of wingless gene expression using short interfering nucleic acid (siNA) |
US20050176025A1 (en) | 2001-05-18 | 2005-08-11 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of B-cell CLL/Lymphoma-2 (BCL-2) gene expression using short interfering nucleic acid (siNA) |
WO2005078095A1 (en) | 2004-02-06 | 2005-08-25 | Dharmacon, Inc. | SNP DISCRIMINATORY siRNA |
US20050186586A1 (en) | 2003-06-02 | 2005-08-25 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of RNAi |
WO2005089224A2 (en) | 2004-03-12 | 2005-09-29 | Alnylam Pharmaceuticals, Inc. | iRNA AGENTS TARGETING VEGF |
US20050227935A1 (en) | 2001-05-18 | 2005-10-13 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA) |
US20050239731A1 (en) | 2001-05-18 | 2005-10-27 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of MAP kinase gene expression using short interfering nucleic acid (siNA) |
US20050245475A1 (en) | 2002-11-14 | 2005-11-03 | Dharmacon, Inc. | Functional and hyperfunctional siRNA directed against Bcl-2 |
US6965025B2 (en) | 2001-12-10 | 2005-11-15 | Isis Pharmaceuticals, Inc. | Antisense modulation of connective tissue growth factor expression |
US20050272680A1 (en) * | 2003-08-18 | 2005-12-08 | Sanjay Bhanot | Modulation of diacylglycerol acyltransferase 2 expression |
WO2005117991A2 (en) | 2004-05-04 | 2005-12-15 | Nastech Pharmaceutical Company Inc. | Compositions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells |
US6994979B2 (en) | 2002-12-12 | 2006-02-07 | The Burnham Institute | Conversion of apoptotic proteins |
US7022837B2 (en) | 2000-11-01 | 2006-04-04 | Bki Holding Corporation | Cellulose ethers and method of preparing the same |
US7022831B1 (en) | 1988-12-22 | 2006-04-04 | The Trustees Of The University Of Pennsylvania | Regulation of BCL-2 gene expression |
WO2006110813A2 (en) | 2005-04-12 | 2006-10-19 | Intradigm Corporation | Composition and methods of rnai therapeutics for treatment of cancer and other neovascularization diseases |
US20060286575A1 (en) | 2005-04-16 | 2006-12-21 | Cylene Pharmaceuticals, Inc. | MCL-1 quadruplex nucleic acids and uses thereof |
US7157570B2 (en) | 2002-03-29 | 2007-01-02 | Yun Yen | Human ribonucleotide reductase M2 subunit |
US20070254850A1 (en) | 2002-10-30 | 2007-11-01 | Judy Lieberman | Methods for Treating and Preventing Apoptosis-Related Diseases Using Rna Interfering Agents |
US20070275465A1 (en) | 2003-06-13 | 2007-11-29 | Alnylam Pharmaceuticals | Double-Stranded Ribonucleic Acid with Increased Effectiveness in an Organism |
US20070281901A1 (en) | 2006-05-19 | 2007-12-06 | Alcon Manufacturing, Ltd. | Rnai-mediated inhibition of tumor necrosis factor alpha-related conditions |
US7521191B2 (en) | 2002-11-14 | 2009-04-21 | Dharmacon, Inc. | siRNA targeting connexin 43 |
Family Cites Families (111)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US702831A (en) * | 1901-08-20 | 1902-06-17 | Samuel Thompson | Combined double escutcheon and paint protector. |
US760807A (en) * | 1903-11-11 | 1904-05-24 | Lee Roberts | Cutting-nippers. |
US5741671A (en) * | 1991-12-12 | 1998-04-21 | The Johns Hopkins University | Isolation cloning and expression of transmembrane water channel aquaporin 1(AQP1) |
US5981175A (en) * | 1993-01-07 | 1999-11-09 | Genpharm Internation, Inc. | Methods for producing recombinant mammalian cells harboring a yeast artificial chromosome |
US5731294A (en) | 1993-07-27 | 1998-03-24 | Hybridon, Inc. | Inhibition of neovasularization using VEGF-specific oligonucleotides |
US6410322B1 (en) | 1993-07-27 | 2002-06-25 | Hybridon Inc | Antisense oligonucleotide inhibition of vascular endothelial growth factor expression |
US5641756A (en) | 1993-07-27 | 1997-06-24 | Hybridon, Inc. | Modified VEGF oligonucleotides |
EP0769552A4 (en) | 1994-06-27 | 1997-06-18 | Toagosei Co Ltd | Antisense nucleic acid compound |
US5830879A (en) | 1995-10-02 | 1998-11-03 | St. Elizabeth's Medical Center Of Boston, Inc. | Treatment of vascular injury using vascular endothelial growth factor |
AU1279397A (en) | 1995-12-08 | 1997-07-03 | Hybridon, Inc. | Modified vegf antisense oligonucleotides |
AU1686997A (en) | 1995-12-08 | 1997-06-27 | Hybridon, Inc. | Modified vegf antisense oligonucleotides for treatment of skin disorders |
CA2251945A1 (en) | 1996-04-17 | 1997-10-23 | Aronex Pharmaceuticals, Inc. | Antisense inhibitors of vascular endothelial growth factor (vefg/vpf) expression |
US20050261485A1 (en) | 1996-05-23 | 2005-11-24 | Toagosei Co., Ltd., A Japan Corporation | Method of producing antisense oligonucleotide |
WO1998027425A1 (en) | 1996-12-19 | 1998-06-25 | Isis Pharmaceuticals, Inc. | Large-scale purification of full length oligonucleotides by solid-liquid affinity extraction |
US6800744B1 (en) * | 1997-07-02 | 2004-10-05 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics |
JPH1142091A (en) | 1997-07-25 | 1999-02-16 | Toagosei Co Ltd | Anti-sense nucleic acid compound |
US6046319A (en) | 1997-10-22 | 2000-04-04 | University Technologies International, Inc. | Antisense oligodeoxynucleotides regulating expression of TNF-α |
AU2572499A (en) | 1998-01-29 | 1999-08-16 | Trustees Of Columbia University In The City Of New York, The | Human hairless gene, protein and uses thereof |
EP1071753A2 (en) | 1998-04-20 | 2001-01-31 | Ribozyme Pharmaceuticals, Inc. | Nucleic acid molecules with novel chemical compositions capable of modulating gene expression |
WO1999055910A1 (en) | 1998-04-24 | 1999-11-04 | Arizona Board Of Regents | Method of inducing apoptosis in a target cell |
CA2334960C (en) | 1998-06-10 | 2012-01-03 | Biognostik Gesellschaft Fur Biomolekulare Diagnostik Mbh | Combination of tgf-.beta. inhibition and immune stimulation to treat hyperproliferative diseases |
WO2000001393A2 (en) | 1998-07-02 | 2000-01-13 | The Trustees Of Columbia University In The City Of New York | OLIGONUCLEOTIDE INHIBITORS OF bcl-xL |
HUP0104302A3 (en) | 1998-11-06 | 2002-11-28 | Abbott Gmbh & Co Kg | Inhibition of the formation of vascular hyperpermeability |
US20040235041A1 (en) * | 1998-11-17 | 2004-11-25 | Shimkets Richard A. | Nucleic acids containing single nucleotide polymorphisms and methods of use thereof |
WO2000032765A2 (en) | 1998-12-04 | 2000-06-08 | Immusol, Inc. | Ribozyme therapy for the treatment and/or prevention of restenosis |
US5958773A (en) | 1998-12-17 | 1999-09-28 | Isis Pharmaceuticals Inc. | Antisense modulation of AKT-1 expression |
WO2000044194A1 (en) | 1999-01-19 | 2000-07-27 | Siemens Aktiengesellschaft | Method for the time synchronisation of a computer network and computer network with time synchronisation |
DE19956568A1 (en) | 1999-01-30 | 2000-08-17 | Roland Kreutzer | Method and medicament for inhibiting the expression of a given gene |
DE19928367A1 (en) * | 1999-06-21 | 2000-12-28 | Will E C H Gmbh & Co | Handling of stacked sheet materials has a facility for stacking and separating into selected stack size |
US6770633B1 (en) | 1999-10-26 | 2004-08-03 | Immusol, Inc. | Ribozyme therapy for the treatment of proliferative skin and eye diseases |
GB9927444D0 (en) | 1999-11-19 | 2000-01-19 | Cancer Res Campaign Tech | Inhibiting gene expression |
DE10160151A1 (en) | 2001-01-09 | 2003-06-26 | Ribopharma Ag | Inhibiting expression of target gene, useful e.g. for inhibiting oncogenes, by administering double-stranded RNA complementary to the target and having an overhang |
US7179796B2 (en) | 2000-01-18 | 2007-02-20 | Isis Pharmaceuticals, Inc. | Antisense modulation of PTP1B expression |
WO2003070918A2 (en) | 2002-02-20 | 2003-08-28 | Ribozyme Pharmaceuticals, Incorporated | Rna interference by modified short interfering nucleic acid |
US8202979B2 (en) | 2002-02-20 | 2012-06-19 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid |
EP1272630A2 (en) | 2000-03-16 | 2003-01-08 | Genetica, Inc. | Methods and compositions for rna interference |
US20030084471A1 (en) | 2000-03-16 | 2003-05-01 | David Beach | Methods and compositions for RNA interference |
US6509559B1 (en) * | 2000-06-20 | 2003-01-21 | Ppt Vision, Inc. | Binary optical grating and method for generating a moire pattern for 3D imaging |
US6521258B1 (en) | 2000-09-08 | 2003-02-18 | Ferro Corporation | Polymer matrices prepared by supercritical fluid processing techniques |
CA2422934A1 (en) | 2000-10-13 | 2002-04-18 | Institut De Cardiologie De Montreal | Antisense oligonucleotides directed toward mammalian vegf receptor genes and uses thereof |
US20030143597A1 (en) | 2000-12-28 | 2003-07-31 | Finney Robert E. | Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis |
US20020160393A1 (en) | 2000-12-28 | 2002-10-31 | Symonds Geoffrey P. | Double-stranded RNA-mediated gene suppression |
WO2002053161A1 (en) | 2000-12-29 | 2002-07-11 | Alteon, Inc. | Method for treating fibrotic diseases or other indications |
JP4080337B2 (en) * | 2001-03-22 | 2008-04-23 | 松下電器産業株式会社 | Positive electrode active material and non-aqueous electrolyte secondary battery including the same |
WO2002085309A2 (en) | 2001-04-24 | 2002-10-31 | Epigenesis Pharmaceuticals, Inc. | Composition, formulations & kits for treatment of respiratory & lung disease with anti-sense oligonucleotides & a bronchodilating agent |
US20070021360A1 (en) | 2001-04-24 | 2007-01-25 | Nyce Jonathan W | Compositions, formulations and kit with anti-sense oligonucleotide and anti-inflammatory steroid and/or obiquinone for treatment of respiratory and lung disesase |
WO2003070910A2 (en) | 2002-02-20 | 2003-08-28 | Ribozyme Pharmaceuticals, Incorporated | INHIBITION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) AND VEGF RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
US20050148530A1 (en) | 2002-02-20 | 2005-07-07 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
DE60237518D1 (en) * | 2001-05-29 | 2010-10-14 | Human Cell Systems Inc | TESTICULAR CARNITINE TRANSPORTER AND ITS GENE |
JP2005500025A (en) | 2001-05-29 | 2005-01-06 | サーナ・セラピューティクス・インコーポレイテッド | Nucleic acid-based regulation of female reproductive diseases and conditions |
MXPA04000224A (en) | 2001-07-10 | 2005-07-25 | 4Sc Ag | Novel compounds as anti-inflammatory, immunomodulatory and anti-proliferatory agents. |
US6734017B2 (en) * | 2001-09-28 | 2004-05-11 | Isis Pharmaceuticals, Inc. | Antisense modulation of vascular endothelial growth factor receptor-2 expression |
JPWO2003038077A1 (en) * | 2001-10-30 | 2005-02-24 | 第一製薬株式会社 | Method for amplifying hematopoietic stem cells |
FR2832154B1 (en) | 2001-11-09 | 2007-03-16 | Centre Nat Rech Scient | OLIGONUCLEOTIDES INHIBITORS AND THEIR USE FOR SPECIFICALLY REPRESSING A GENE |
US20030186903A1 (en) | 2001-11-23 | 2003-10-02 | Isis Pharmaceuticals Inc. | Antisense modulation of MyD88 expression |
CA2468326C (en) | 2001-11-26 | 2015-11-17 | Pierre Legrain | Protein-protein interactions in human immunodeficiency virus |
KR100441894B1 (en) * | 2002-01-26 | 2004-07-27 | 한국전자통신연구원 | Micro-integrated near-field optical recording head and optical recording system using the same |
US20030224512A1 (en) | 2002-05-31 | 2003-12-04 | Isis Pharmaceuticals Inc. | Antisense modulation of beta-site APP-cleaving enzyme expression |
AU2003237570A1 (en) | 2002-05-13 | 2003-11-11 | Salviac Limited | Catheter system with procedural catheter and embolic proctection system |
US20040102391A1 (en) | 2002-11-21 | 2004-05-27 | Isis Pharmaceuticals Inc. | Modulation of Gankyrin expression |
CA2489726A1 (en) | 2002-06-18 | 2003-12-24 | Irm Llc | Diagnosis and treatment of chemoresistant tumors |
US7148342B2 (en) | 2002-07-24 | 2006-12-12 | The Trustees Of The University Of Pennyslvania | Compositions and methods for sirna inhibition of angiogenesis |
GB0218010D0 (en) * | 2002-08-05 | 2002-09-11 | Ciba Spec Chem Water Treat Ltd | Production of a fermentation product |
WO2004027030A2 (en) | 2002-09-18 | 2004-04-01 | Isis Pharmaceuticals, Inc. | Efficient reduction of target rna’s by single- and double-stranded oligomeric compounds |
US7612196B2 (en) | 2002-11-14 | 2009-11-03 | Dharmacon, Inc. | siRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B) |
US7781575B2 (en) * | 2002-11-14 | 2010-08-24 | Dharmacon, Inc. | siRNA targeting tumor protein 53 (p53) |
US7691998B2 (en) | 2002-11-14 | 2010-04-06 | Dharmacon, Inc. | siRNA targeting nucleoporin 62kDa (Nup62) |
US7977471B2 (en) | 2002-11-14 | 2011-07-12 | Dharmacon, Inc. | siRNA targeting TNFα |
US7635770B2 (en) | 2002-11-14 | 2009-12-22 | Dharmacon, Inc. | siRNA targeting protein kinase N-3 (PKN-3) |
US7592442B2 (en) | 2002-11-14 | 2009-09-22 | Dharmacon, Inc. | siRNA targeting ribonucleotide reductase M2 polypeptide (RRM2 or RNR-R2) |
US7582747B2 (en) | 2002-11-14 | 2009-09-01 | Dharmacon, Inc. | siRNA targeting inner centromere protein antigens (INCENP) |
US7619081B2 (en) | 2002-11-14 | 2009-11-17 | Dharmacon, Inc. | siRNA targeting coatomer protein complex, subunit beta 2 (COPB2) |
US7250496B2 (en) | 2002-11-14 | 2007-07-31 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory genes and uses thereof |
US7951935B2 (en) | 2002-11-14 | 2011-05-31 | Dharmacon, Inc. | siRNA targeting v-myc myelocytomatosis viral oncogene homolog (MYC) |
WO2004061423A2 (en) * | 2003-01-06 | 2004-07-22 | Wyeth | Compositions and methods for diagnosing and treating colon cancers |
FR2835837B1 (en) | 2003-02-06 | 2007-03-16 | Centre Nat Rech Scient | INHIBITORY OLIGONUCLEOTIDES AND THEIR USE FOR SPECIFICALLY REPRESSING A GENE ENCODING A GROWTH FACTOR |
FR2835838B1 (en) | 2003-02-06 | 2007-11-16 | Centre Nat Rech Scient | OLIGONUCLEOTIDES INHIBITORS AND THEIR USE FOR SPECIFICALLY REPRESSING A GENE ENCODING A TRANSCRIPTION FACTOR |
CA2515677A1 (en) * | 2003-02-11 | 2004-08-26 | Wyeth | Methods for monitoring drug activities in vivo |
WO2004076639A2 (en) * | 2003-02-26 | 2004-09-10 | Wyeth | Use of gene expression profiling in the diagnosis and treatment of lupus nephritis and systemic lupus erythematosus |
ATE479752T1 (en) * | 2003-03-07 | 2010-09-15 | Alnylam Pharmaceuticals Inc | THERAPEUTIC COMPOSITIONS |
AU2003224132A1 (en) | 2003-04-24 | 2004-11-19 | Galapagos Genomics N.V. | Effective sirna knock-down constructs |
US7399853B2 (en) | 2003-04-28 | 2008-07-15 | Isis Pharmaceuticals | Modulation of glucagon receptor expression |
KR20060063788A (en) | 2003-05-30 | 2006-06-12 | 니뽄 신야쿠 가부시키가이샤 | Oligonucleic acid-bearing composite and pharmaceutical composition containing the composite |
ES2712695T3 (en) | 2003-06-02 | 2019-05-14 | Univ Massachusetts | Methods and compositions to control the efficiency of RNA silencing |
DE10341333B4 (en) | 2003-09-08 | 2006-06-08 | Siemens Ag | Piezoelectric actuator and method for producing a piezoelectric actuator |
CN1886793A (en) | 2003-11-27 | 2006-12-27 | 皇家飞利浦电子股份有限公司 | Method and system for chapter marker and title boundary insertion in dv video |
KR100586654B1 (en) * | 2003-12-19 | 2006-06-07 | 이처닷컴 주식회사 | Wireless banking system and wireless banking method using mobile phone |
WO2005062937A2 (en) | 2003-12-22 | 2005-07-14 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended sirna |
JP2005233933A (en) * | 2004-01-19 | 2005-09-02 | Nec Electronics Corp | Combination test method and testing device |
KR101054402B1 (en) * | 2004-02-06 | 2011-08-04 | 엘지전자 주식회사 | Pulley assembly of washing machine |
KR101147147B1 (en) | 2004-04-01 | 2012-05-25 | 머크 샤프 앤드 돔 코포레이션 | Modified polynucleotides for reducing off-target effects in rna interference |
CA2557532A1 (en) | 2004-04-23 | 2005-11-10 | Angela M. Christiano | Inhibition of hairless protein mrna |
CN101052717A (en) | 2004-05-11 | 2007-10-10 | α基因株式会社 | Polynucleotide causing RNA interfere and method of regulating gene expression with the use of the same |
US7605250B2 (en) | 2004-05-12 | 2009-10-20 | Dharmacon, Inc. | siRNA targeting cAMP-specific phosphodiesterase 4D |
LT1799269T (en) | 2004-09-28 | 2016-10-25 | Quark Pharmaceuticals, Inc. | Oligoribonucleotides and methods of use thereof for treatment of alopecia, acute renal failure and other diseases |
WO2006053430A1 (en) * | 2004-11-17 | 2006-05-26 | Protiva Biotherapeutics, Inc. | Sirna silencing of apolipoprotein b |
US7879992B2 (en) * | 2005-01-31 | 2011-02-01 | Isis Pharmaceuticals, Inc. | Modification of MyD88 splicing using modified oligonucleotides |
US7150451B2 (en) * | 2005-02-18 | 2006-12-19 | Gm Global Technology Operations, Inc. | Air spring and jounce shock assembly |
US8268629B2 (en) * | 2005-06-21 | 2012-09-18 | dTEC Systems L.L.C. | Method for the measurement of water and water-soluble components in non-aqueous liquids |
ATE404376T1 (en) | 2005-09-29 | 2008-08-15 | Brother Ind Ltd | INK CARTRIDGE, SET OF INK CARTRIDGES AND INK JET RECORDING SYSTEM |
US7825099B2 (en) | 2006-01-20 | 2010-11-02 | Quark Pharmaceuticals, Inc. | Treatment or prevention of oto-pathologies by inhibition of pro-apoptotic genes |
WO2007087451A2 (en) | 2006-01-25 | 2007-08-02 | University Of Massachusetts | Compositions and methods for enhancing discriminatory rna interference |
US7910566B2 (en) | 2006-03-09 | 2011-03-22 | Quark Pharmaceuticals Inc. | Prevention and treatment of acute renal failure and other kidney diseases by inhibition of p53 by siRNA |
US8523707B2 (en) * | 2006-05-31 | 2013-09-03 | Bridgestone Sports Co., Ltd. | Multi-piece solid golf ball |
US8138160B2 (en) | 2006-08-03 | 2012-03-20 | Warsaw Orthopedic, Inc. | Reagents, methods and systems to suppress pro-inflammatory cytokines |
AU2007286545A1 (en) * | 2006-08-24 | 2008-02-28 | Alcon Research, Ltd. | RNAi-mediated inhibition of gremlin for treatment of IOP-related conditions |
JP2010507387A (en) * | 2006-10-25 | 2010-03-11 | クアーク・ファーマスーティカルス、インコーポレイテッド | Novel siRNA and method of using the same |
SI2170403T1 (en) | 2007-06-27 | 2014-07-31 | Quark Pharmaceuticals, Inc. | Compositions and methods for inhibiting expression of pro-apoptotic genes |
US7572747B2 (en) * | 2007-09-27 | 2009-08-11 | Alfred University | Optical glass |
CN103898110A (en) | 2007-10-03 | 2014-07-02 | 夸克制药公司 | Novel sirna structures |
CN101640467B (en) | 2008-07-28 | 2012-05-30 | 鸿富锦精密工业(深圳)有限公司 | Shrapnel and voice coil motor |
-
2004
- 2004-05-12 WO PCT/US2004/014885 patent/WO2006006948A2/en active Application Filing
-
2006
- 2006-11-03 US US11/593,100 patent/US7615541B2/en not_active Expired - Fee Related
- 2006-11-08 US US11/594,530 patent/US7608706B2/en not_active Expired - Fee Related
- 2006-11-08 US US11/594,666 patent/US20070128641A1/en not_active Abandoned
- 2006-11-09 US US11/598,179 patent/US7541453B2/en not_active Expired - Fee Related
- 2006-11-09 US US11/595,698 patent/US7598369B2/en not_active Expired - Fee Related
-
2007
- 2007-03-28 US US11/729,388 patent/US20070185317A1/en not_active Abandoned
- 2007-03-29 US US11/729,924 patent/US20080015114A1/en not_active Abandoned
- 2007-03-30 US US11/731,843 patent/US7569684B2/en not_active Expired - Fee Related
- 2007-03-30 US US11/731,875 patent/US20070299253A1/en not_active Abandoned
- 2007-03-30 US US11/731,890 patent/US20080045703A1/en not_active Abandoned
- 2007-03-30 US US11/731,894 patent/US7521191B2/en not_active Expired - Fee Related
- 2007-04-03 US US11/732,413 patent/US20070238868A1/en not_active Abandoned
- 2007-04-03 US US11/732,457 patent/US7638621B2/en not_active Expired - Fee Related
- 2007-04-04 US US11/732,809 patent/US20070255046A1/en not_active Abandoned
- 2007-04-04 US US11/732,810 patent/US20070219362A1/en not_active Abandoned
- 2007-04-06 US US11/784,536 patent/US20070179286A1/en not_active Abandoned
- 2007-04-06 US US11/784,559 patent/US20070213520A1/en not_active Abandoned
- 2007-04-09 US US11/784,752 patent/US20070213521A1/en not_active Abandoned
- 2007-04-09 US US11/784,756 patent/US20070232797A1/en not_active Abandoned
- 2007-04-09 US US11/784,755 patent/US7550572B2/en not_active Expired - Fee Related
- 2007-05-29 US US11/807,577 patent/US20070260048A1/en not_active Abandoned
- 2007-05-29 US US11/807,486 patent/US7745610B2/en active Active
- 2007-05-29 US US11/807,530 patent/US20070255047A1/en not_active Abandoned
- 2007-06-01 US US11/809,909 patent/US20070255048A1/en not_active Abandoned
- 2007-06-04 US US11/810,074 patent/US20070276135A1/en not_active Abandoned
- 2007-06-05 US US11/810,382 patent/US20070260047A1/en not_active Abandoned
- 2007-06-05 US US11/810,383 patent/US7595388B2/en not_active Expired - Fee Related
- 2007-06-05 US US11/810,384 patent/US20070260049A1/en not_active Abandoned
- 2007-06-06 US US11/810,673 patent/US20070293664A1/en not_active Abandoned
- 2007-06-06 US US11/810,672 patent/US20070255050A1/en not_active Abandoned
- 2007-06-07 US US11/811,003 patent/US20070287833A1/en not_active Abandoned
- 2007-06-07 US US11/811,012 patent/US20070260050A1/en not_active Abandoned
- 2007-06-07 US US11/811,005 patent/US20070265437A1/en not_active Abandoned
- 2007-06-08 US US11/811,424 patent/US20070244312A1/en not_active Abandoned
- 2007-06-08 US US11/811,423 patent/US7645870B2/en not_active Expired - Lifetime
- 2007-06-08 US US11/811,209 patent/US20080085997A1/en not_active Abandoned
- 2007-06-12 US US11/811,925 patent/US20070260051A1/en not_active Abandoned
- 2007-06-12 US US11/811,954 patent/US20070249819A1/en not_active Abandoned
- 2007-06-12 US US11/811,950 patent/US20070260052A1/en not_active Abandoned
- 2007-06-12 US US11/811,929 patent/US20070255051A1/en not_active Abandoned
- 2007-06-14 US US11/818,547 patent/US20070255052A1/en not_active Abandoned
- 2007-06-15 US US11/818,936 patent/US7598370B2/en not_active Expired - Lifetime
- 2007-06-15 US US11/818,938 patent/US7678896B2/en not_active Expired - Lifetime
- 2007-07-23 US US11/880,624 patent/US20080027215A1/en not_active Abandoned
- 2007-07-25 US US11/880,965 patent/US7579458B2/en not_active Expired - Fee Related
- 2007-07-27 US US11/881,772 patent/US20080027216A1/en not_active Abandoned
- 2007-07-27 US US11/881,767 patent/US20080039617A1/en not_active Abandoned
- 2007-10-22 US US11/975,902 patent/US20080097091A1/en not_active Abandoned
- 2007-10-23 US US11/977,128 patent/US20080097092A1/en not_active Abandoned
- 2007-10-24 US US11/977,347 patent/US20080076908A1/en not_active Abandoned
- 2007-10-25 US US11/977,675 patent/US20080071073A1/en not_active Abandoned
- 2007-10-25 US US11/977,558 patent/US20080097089A1/en not_active Abandoned
- 2007-10-26 US US11/978,125 patent/US20080086002A1/en not_active Abandoned
- 2007-10-26 US US11/978,097 patent/US7638622B2/en not_active Expired - Fee Related
- 2007-10-26 US US11/978,120 patent/US20080081904A1/en not_active Abandoned
- 2007-10-26 US US11/978,070 patent/US7582746B2/en not_active Expired - Fee Related
- 2007-10-26 US US11/978,106 patent/US7655789B2/en not_active Expired - Lifetime
- 2007-10-26 US US11/978,107 patent/US7605252B2/en not_active Expired - Fee Related
- 2007-10-29 US US11/978,398 patent/US7709629B2/en not_active Expired - Lifetime
- 2007-10-29 US US11/978,475 patent/US20080113372A1/en not_active Abandoned
- 2007-10-29 US US11/978,457 patent/US20080113371A1/en not_active Abandoned
- 2007-10-29 US US11/978,518 patent/US7632938B2/en not_active Expired - Fee Related
- 2007-10-29 US US11/978,487 patent/US20080113374A1/en not_active Abandoned
- 2007-10-29 US US11/978,455 patent/US7795421B2/en not_active Expired - Fee Related
- 2007-10-29 US US11/978,476 patent/US7635771B2/en not_active Expired - Fee Related
- 2007-10-30 US US11/980,102 patent/US7662950B2/en not_active Expired - Fee Related
- 2007-10-30 US US11/980,300 patent/US7592443B2/en not_active Expired - Fee Related
- 2007-10-30 US US11/980,263 patent/US7632939B2/en not_active Expired - Fee Related
-
2009
- 2009-01-23 US US12/321,749 patent/US7666853B2/en not_active Expired - Fee Related
- 2009-02-02 US US12/322,387 patent/US7589191B2/en not_active Expired - Fee Related
- 2009-05-28 US US12/455,098 patent/US7741470B2/en not_active Expired - Fee Related
- 2009-07-24 US US12/460,876 patent/US20100004141A1/en not_active Abandoned
- 2009-07-28 US US12/462,029 patent/US7745612B2/en not_active Expired - Fee Related
- 2009-08-04 US US12/462,420 patent/US7737267B2/en not_active Expired - Fee Related
- 2009-08-10 US US12/462,820 patent/US8022198B2/en not_active Expired - Fee Related
- 2009-09-03 US US12/584,352 patent/US8222395B2/en not_active Expired - Fee Related
- 2009-09-11 US US12/584,850 patent/US7897754B2/en not_active Expired - Fee Related
- 2009-09-17 US US12/586,167 patent/US7855186B2/en not_active Expired - Fee Related
- 2009-10-29 US US12/589,879 patent/US8039610B2/en not_active Expired - Fee Related
- 2009-11-02 US US12/590,097 patent/US7816512B2/en not_active Expired - Fee Related
- 2009-11-04 US US12/590,252 patent/US7829696B2/en not_active Expired - Fee Related
- 2009-12-03 US US12/592,872 patent/US8304528B2/en active Active
- 2009-12-08 US US12/653,120 patent/US8022199B2/en not_active Expired - Fee Related
- 2009-12-11 US US12/653,402 patent/US7807820B2/en not_active Expired - Fee Related
- 2009-12-23 US US12/655,107 patent/US7833989B2/en not_active Expired - Fee Related
-
2010
- 2010-01-21 US US12/657,448 patent/US8067576B2/en not_active Expired - Lifetime
- 2010-03-01 US US12/660,582 patent/US8247169B2/en not_active Expired - Lifetime
- 2010-04-07 US US12/798,603 patent/US8030476B2/en not_active Expired - Fee Related
- 2010-04-12 US US12/798,802 patent/US7935813B2/en not_active Expired - Fee Related
- 2010-04-13 US US12/798,906 patent/US8236942B2/en active Active
- 2010-04-30 US US12/799,758 patent/US8217162B2/en not_active Expired - Lifetime
- 2010-05-05 US US12/799,975 patent/US20100267587A1/en not_active Abandoned
- 2010-07-12 US US12/804,014 patent/US8071754B2/en not_active Expired - Fee Related
- 2010-08-17 US US12/806,570 patent/US7999097B2/en not_active Expired - Fee Related
- 2010-09-08 US US12/807,526 patent/US8222396B2/en not_active Expired - Fee Related
- 2010-09-20 US US12/924,078 patent/US8268985B2/en not_active Expired - Lifetime
- 2010-10-01 US US12/924,653 patent/US8138329B2/en not_active Expired - Lifetime
- 2010-11-08 US US12/927,144 patent/US8314229B2/en not_active Expired - Fee Related
-
2011
- 2011-07-01 US US13/135,336 patent/US8293887B2/en not_active Expired - Lifetime
- 2011-08-10 US US13/136,780 patent/US8633306B2/en not_active Expired - Fee Related
- 2011-08-11 US US13/136,812 patent/US8426579B2/en not_active Expired - Fee Related
- 2011-08-23 US US13/199,240 patent/US20110319297A1/en not_active Abandoned
- 2011-09-13 US US13/199,946 patent/US20120015850A1/en not_active Abandoned
- 2011-10-27 US US13/317,752 patent/US8232386B2/en not_active Expired - Lifetime
-
2012
- 2012-02-14 US US13/385,320 patent/US8461326B2/en not_active Expired - Fee Related
- 2012-06-06 US US13/489,725 patent/US20120252873A1/en not_active Abandoned
- 2012-06-12 US US13/494,360 patent/US20120258888A1/en not_active Abandoned
- 2012-06-15 US US13/524,015 patent/US8575329B2/en not_active Expired - Fee Related
- 2012-06-28 US US13/536,005 patent/US8445668B2/en not_active Expired - Fee Related
- 2012-07-02 US US13/539,630 patent/US20120270751A1/en not_active Abandoned
- 2012-07-05 US US13/542,332 patent/US20120283311A1/en not_active Abandoned
- 2012-07-18 US US13/551,794 patent/US8658784B2/en not_active Expired - Fee Related
- 2012-09-13 US US13/613,910 patent/US20130023446A1/en not_active Abandoned
- 2012-10-01 US US13/632,519 patent/US20130059760A1/en not_active Abandoned
- 2012-10-09 US US13/647,869 patent/US8658785B1/en not_active Expired - Fee Related
-
2013
- 2013-03-20 US US13/847,544 patent/US8883998B2/en not_active Expired - Fee Related
- 2013-04-22 US US13/867,175 patent/US20130225447A1/en not_active Abandoned
- 2013-12-06 US US14/099,339 patent/US8907077B2/en not_active Expired - Fee Related
Patent Citations (71)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7022831B1 (en) | 1988-12-22 | 2006-04-04 | The Trustees Of The University Of Pennsylvania | Regulation of BCL-2 gene expression |
US20020086321A1 (en) | 1993-02-02 | 2002-07-04 | Craig Ruth W. | Myeloid cell leukemia associated gene MCL-1 |
US6291642B1 (en) | 1994-02-14 | 2001-09-18 | Amgen Inc. | Mammalian cell cycle protein |
US6566127B1 (en) | 1995-10-26 | 2003-05-20 | Ribozyme Pharmaceuticals, Inc. | Method and reagent for the treatment of diseases or conditions related to levels of vascular endothelial growth factor receptor |
US20020081578A1 (en) | 1995-12-18 | 2002-06-27 | Gregory Plowman | Diagnosis and treatment of aur1 and/or aur2 related disorders |
WO1998000532A2 (en) | 1996-07-01 | 1998-01-08 | Wright Jim A | Oligonucleotides from the untranslated regions of ribonucleotide reductase and their use to modulate cell growth |
US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
US6111086A (en) | 1998-02-27 | 2000-08-29 | Scaringe; Stephen A. | Orthoester protecting groups |
US20030228597A1 (en) | 1998-04-13 | 2003-12-11 | Cowsert Lex M. | Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation |
WO2000020645A1 (en) | 1998-10-05 | 2000-04-13 | Isis Pharmaceuticals, Inc. | ANTISENSE OLIGONUCLEOTIDE MODULATION OF TUMOR NECROSIS FACTOR-α (TNF-α) EXPRESSION |
US6172216B1 (en) | 1998-10-07 | 2001-01-09 | Isis Pharmaceuticals Inc. | Antisense modulation of BCL-X expression |
WO2000021559A2 (en) | 1998-10-09 | 2000-04-20 | Musc Foundation For Research Development | Blocking factor b to treat complement-mediated immune disease |
US20040204380A1 (en) | 1999-01-07 | 2004-10-14 | Ackermann Elizabeth J | Antisense modulation of novel anti-apoptotic bcl-2-related proteins |
US6001992A (en) | 1999-01-07 | 1999-12-14 | Isis Pharmaceuticals Inc. | Antisense modulation of novel anti-apoptotic bcl-2-related proteins |
WO2000076497A1 (en) | 1999-06-14 | 2000-12-21 | Cancer Research Ventures Limited | Cancer therapy |
US20020086356A1 (en) | 2000-03-30 | 2002-07-04 | Whitehead Institute For Biomedical Research | RNA sequence-specific mediators of RNA interference |
US7022837B2 (en) | 2000-11-01 | 2006-04-04 | Bki Holding Corporation | Cellulose ethers and method of preparing the same |
US20040259247A1 (en) | 2000-12-01 | 2004-12-23 | Thomas Tuschl | Rna interference mediating small rna molecules |
WO2002044321A2 (en) | 2000-12-01 | 2002-06-06 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Rna interference mediating small rna molecules |
US20020150945A1 (en) | 2000-12-28 | 2002-10-17 | Cell Therapeutics, Inc. | Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis |
US20030143732A1 (en) | 2001-04-05 | 2003-07-31 | Kathy Fosnaugh | RNA interference mediated inhibition of adenosine A1 receptor (ADORA1) gene expression using short interfering RNA |
US20030087259A1 (en) | 2001-04-18 | 2003-05-08 | Clancy Brian M. | Methods and compositions for regulating bone and cartilage formation |
US20050239731A1 (en) | 2001-05-18 | 2005-10-27 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of MAP kinase gene expression using short interfering nucleic acid (siNA) |
US20050227935A1 (en) | 2001-05-18 | 2005-10-13 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA) |
US20050176025A1 (en) | 2001-05-18 | 2005-08-11 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of B-cell CLL/Lymphoma-2 (BCL-2) gene expression using short interfering nucleic acid (siNA) |
US20050130181A1 (en) | 2001-05-18 | 2005-06-16 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of wingless gene expression using short interfering nucleic acid (siNA) |
US20030105051A1 (en) | 2001-05-29 | 2003-06-05 | Mcswiggen James | Nucleic acid treatment of diseases or conditions related to levels of HER2 |
WO2003035870A1 (en) | 2001-10-26 | 2003-05-01 | Ribopharma Ag | Drug for treating a carcinoma of the pancreas |
WO2003035869A1 (en) | 2001-10-26 | 2003-05-01 | Ribopharma Ag | Use of a double-stranded ribonucleic acid for specifically inhibiting the expression of a given target gene |
US20030157030A1 (en) | 2001-11-02 | 2003-08-21 | Insert Therapeutics, Inc. | Methods and compositions for therapeutic use of rna interference |
US20040063654A1 (en) | 2001-11-02 | 2004-04-01 | Davis Mark E. | Methods and compositions for therapeutic use of RNA interference |
US6965025B2 (en) | 2001-12-10 | 2005-11-15 | Isis Pharmaceuticals, Inc. | Antisense modulation of connective tissue growth factor expression |
US20040054155A1 (en) | 2002-02-01 | 2004-03-18 | Sequitur, Inc. | Oligonucleotide compositions with enhanced efficiency |
WO2003070969A2 (en) | 2002-02-20 | 2003-08-28 | Sirna Therapeutics, Inc. | RNA INTERFERENCE MEDIATED INHIBITION OF BCL2 GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
WO2003070897A2 (en) | 2002-02-20 | 2003-08-28 | Ribozyme Pharmaceuticals, Incorporated | RNA INTERFERENCE MEDIATED INHIBITION OF TNF AND TNF RECEPTOR SUPERFAMILY GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
WO2003072704A2 (en) | 2002-02-20 | 2003-09-04 | Sirna Therapeutics, Inc. | RNA INTERFERENCE MEDIATED INHIBITION OF PLATELET DERIVED GROWTH FACTOR (PDGF) AND PLATELET DERIVED GROWTH FACTOR RECEPTOR (PDGFR) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
US20040219671A1 (en) | 2002-02-20 | 2004-11-04 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of parkinson disease using short interfering nucleic acid (siNA) |
US20050048529A1 (en) | 2002-02-20 | 2005-03-03 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA) |
US20040248296A1 (en) * | 2002-03-20 | 2004-12-09 | Beresford Paul J. | HIV therapeutic |
US7157570B2 (en) | 2002-03-29 | 2007-01-02 | Yun Yen | Human ribonucleotide reductase M2 subunit |
US20040077574A1 (en) | 2002-05-23 | 2004-04-22 | Ceptyr, Inc. | Modulation of biological signal transduction by RNA interference |
US20050107328A1 (en) | 2002-07-30 | 2005-05-19 | Jacqueline Wyatt | Antisense modulation of polo-like kinase expression |
US20040029275A1 (en) | 2002-08-10 | 2004-02-12 | David Brown | Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs |
WO2004031237A1 (en) | 2002-09-30 | 2004-04-15 | Oncotherapy Science, Inc. | Genes and polypeptides relating to human myeloid leukemia |
US20070254850A1 (en) | 2002-10-30 | 2007-11-01 | Judy Lieberman | Methods for Treating and Preventing Apoptosis-Related Diseases Using Rna Interfering Agents |
US20040180357A1 (en) | 2002-11-01 | 2004-09-16 | The Trustees Of The University Of Pennsylvania | Compositions and methods for siRNA inhibition of HIF-1 alpha |
US20040192629A1 (en) | 2002-11-04 | 2004-09-30 | University Of Massachusetts | Allele-specific RNA interference |
US20050245475A1 (en) | 2002-11-14 | 2005-11-03 | Dharmacon, Inc. | Functional and hyperfunctional siRNA directed against Bcl-2 |
US20070031844A1 (en) | 2002-11-14 | 2007-02-08 | Anastasia Khvorova | Functional and hyperfunctional siRNA |
US7521191B2 (en) | 2002-11-14 | 2009-04-21 | Dharmacon, Inc. | siRNA targeting connexin 43 |
US7514550B2 (en) | 2002-11-14 | 2009-04-07 | Dharmacon, Inc. | siRNA targeting myeloid cell leukemia sequence 1 |
US7511132B2 (en) | 2002-11-14 | 2009-03-31 | Dharmacon, Inc. | Functional and hyperfunctional siRNA directed against Bcl-2 |
US7507811B2 (en) | 2002-11-14 | 2009-03-24 | Dharmacon, Inc. | siRNA targeting KRAS |
WO2004046324A2 (en) | 2002-11-15 | 2004-06-03 | University Of Massachusetts | Allele-targeted rna interference |
US20040101857A1 (en) | 2002-11-23 | 2004-05-27 | Isis Pharmaceuticals Inc. | Modulation of cytokine-inducible kinase expression |
WO2004048511A2 (en) | 2002-11-26 | 2004-06-10 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel viral regulatory genes and uses thereof |
US6994979B2 (en) | 2002-12-12 | 2006-02-07 | The Burnham Institute | Conversion of apoptotic proteins |
US20040248299A1 (en) | 2002-12-27 | 2004-12-09 | Sumedha Jayasena | RNA interference |
WO2004090105A2 (en) | 2003-04-02 | 2004-10-21 | Dharmacon, Inc. | Modified polynucleotides for use in rna interference |
US20050181382A1 (en) | 2003-06-02 | 2005-08-18 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of RNAi |
US20050186586A1 (en) | 2003-06-02 | 2005-08-25 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of RNAi |
WO2005001043A2 (en) | 2003-06-02 | 2005-01-06 | University Of Massachusetts | METHODS AND COMPOSITIONS FOR ENHANCING THE EFFICACY AND SPECIFICITY OF FNAi |
US20070275465A1 (en) | 2003-06-13 | 2007-11-29 | Alnylam Pharmaceuticals | Double-Stranded Ribonucleic Acid with Increased Effectiveness in an Organism |
US20050272680A1 (en) * | 2003-08-18 | 2005-12-08 | Sanjay Bhanot | Modulation of diacylglycerol acyltransferase 2 expression |
WO2005078095A1 (en) | 2004-02-06 | 2005-08-25 | Dharmacon, Inc. | SNP DISCRIMINATORY siRNA |
WO2005089224A2 (en) | 2004-03-12 | 2005-09-29 | Alnylam Pharmaceuticals, Inc. | iRNA AGENTS TARGETING VEGF |
WO2005117991A2 (en) | 2004-05-04 | 2005-12-15 | Nastech Pharmaceutical Company Inc. | Compositions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells |
WO2006015389A2 (en) | 2004-08-04 | 2006-02-09 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of rna silencing |
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Non-Patent Citations (99)
Title |
---|
Abeliovich, et al. (2000) Mice Lacking Alph-Synuclein Display Functional Deficits in the Nigrostriatal Dopamine System. Neuron v.25:239-52. |
Abifadel et al., Mutations in PCSK9 cause autosomal dominant hypercholesterolemia, 2003, Nature Genetics, vol. 34, pp. 154-156. |
Amarzguioui M et al, Tolerance for mutations and chemical modifications in a siRNA Nucleic Acids Research vol. 31, No. 2, Jan. 15, 2003 pp. 589-595 , XP002270887 ISSN: 0305-1048. |
Amarzguioui, et al., Secondary Structure Prediction and in vitro Accessibility of mRNA as Tools in the Selection of Target Sites for Ribozymes, Nucleic Acids Research, 2000, vol. 28, No. 21, pp. 4113-4124. |
Amarzguioui, Secondary structure prediction and in vitro accessibility of mRNA as tools in the selection of target sites for ribozymes (2000) vol. 29, No. 21, 4113-4124. |
Bass, B.L., (2001) The Short Answer, Nature V.411:428-29. |
Betrand (2002) Comparison of Antisense Oligonucleotides and siRNAs in cell culture and in vivo, Biotechnical and Biophysicial Research Communication 296:1000-1004. |
Boutla, Shot 5'-phosphorylated double-stranded RNAs induce RNA interference in Drosophila, 1776-1780 (Brief Communication) (2001). |
Brown et al. (1997) Identification and cDNA Cloning of a Novel Mammalian C2 Domain-Containing Phosphoinositide 3-kinase, HsC2-PI3K. Biochemical and Biophysical Research Communications. 233, 537-544. |
Brummelkarna et al. A System for Stable Expression of Short lntereferring RNAs in Mammalian Cells, Science vol. 296 pp. 550-553 (2002). |
Brusca, John S., PCT International Search Report, United States Patent and Trademark Office, Sep. 24, 2007. |
Cahill et al. (1999) Characterization of MAD2B and Other Mitotic Spindle Checkpoint Genes. Genomics. 58:181-187. |
Caplen et al. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate systems, Proc. Natl. Acad. Sci USA vol. 98, pp. 9742-9747 (2001). |
Caplen, dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for analysis of RNA interference, Jul. 11, 2000; Gene; 252(1-2):95-105. |
Caplen, RNAi as a gene therapy approach. Expert Opin. Ther. 3(4):575-586. (2003). |
Chalk, siRNA db: a database of siRNA sequences. Nucleic Acids Research. vol. 33 pp. D131-D134 (2004). |
Chen et al. (2005) TSSK5, A Novel Member of the Testis-Specific Serine/Threonin Kinase Family, Phosphorylates CREB at Ser-122, and Stimulates the CRE-CREB Responsive Pathway. Biochemical and Biophysical Research Communications. 333: 742-749. |
Cherry, Michael J. (1995) Computer Manipulation of DNA and Protein Sequences. Current Protocols in Molecular Biology. 7.7.1-7.7.23. |
Chi, Genomewide view of gene silencing by small interfering RNAs. PNAS vol. 100, No. 11, pp. 6343-6346 (2003). |
Chinault, Overlap Hybridization Screening: Isolation and Characterization of Overlapping DNA Framgments Surroungin the leu2 Gene on Yeast Chromosome III (1979), 5(2):111-26. |
Daniel et al., Specific association of Type I with Ran GTPase in lipopolysacchardie-mediated differentiation, Oncogene 2001, vol. 20: 2618-2625. |
Dodelet et al. (2000) Eph Receptors and Ephrin Ligands: embryogenesis to tumorigenesis. Oncogene 19:5614-5619. |
Domin et al.(1997) Cloning of Human Phosphoinositide 3-Kinase with a C2 Domain that Displays Reduced Sensitivity to the Inhibitor Wortmannin. Biochem. J., v.325:139-47. |
Dottori, et al. (1998) EphA4 (Sek1) receptor tyrosine kinase is required for the development of the corticospinal tract, Proc. Natl. Acad. Sci. vol. 95, pp. 13248-13253, Neurobiology. |
Ekholm, et al.(2004) Deregulation of Cyclin E in Human Cells Interferes with Prereplication Complex Assembly. The Journal of Cell Biology. vol. 165, pp. 789-800. |
El Touny, et al. (2006) Identification of Both Myt-I and Wee-I as Necessary Mediators of the P2l-Independent Inactivation of the Cdc-2/Cyclin BI complex and Growth Inhibition of TRAMP Cancer Cells by Genistein. The Prostate, vol. 66:1542-1555. |
Elabashir, et al., Functional Anatomy of siRNAs for Mediating Efficient RNAi in Drosophila melanogaster Embryo Lysate, European Molecular Biology Organization, 2001, pp. 6877-6888. |
Elbashir et al. (2001) Duplexes of 21-Nucleotide RNAs Mediate RNA Interference in Cultured Mammalian Cells. Nature vol. 411, pp. 494-498. |
Elbashir, et al., Analysis of Gene Function in Somatic mammalian Cells using small Interfering RNAs, Methods, 2000, vol. 26, pp. 199-213. |
Epps-Ford, Janet L., PCT International Search Report, United States Patent and Trademark Office, Feb. 25, 2005. |
European Patent Office, European Patent Application No. 03786798.3 Office Action dated Nov. 15, 2007. |
Far, R-K, The activity of siRNA in mammalian cells is related to structural target accessibility: a comparison with antisense oligonucleotides (2003) Nucleic Acids Research. vol. 31, No. 15, pp. 4417-4424. |
Feng, et al. (2003) Inhibiting the Expression of DNA Replication-Initiation Proteins Induces Apoptosis in Human Cancer Cells. Cancer Research, vol. 63, pp. 7356-7364. |
Fox, et al. (1995) cDNA Cloning and Tissue Distribution of Five Human EPH-like Receptor Protein-tyrosine kinases, Oncogene; 10(5): 897-905. |
Futami et al. (2002) Induction of Apoptosis in Hela Cells with siRNA Expression Vector Targeted Against BLC-2. Nucleic Acids Research Supplement No. 2:251-2. |
Genbank Accession NM 001790 Oct. 10, 2008. |
Genbank Accession No. NM-002645.2, http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=157671928. Accessed on Jul. 31, 2008. |
GenBank Accession No. NM-00345, http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6806896. Accessed on Aug. 1, 2008. |
Genbank Accession No. NM-138578, http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=20336334. Accessed on Aug. 11, 2008. |
Genbank Accession Z15005.1 (1993). |
Hammond et al., Post-transcriptional gene silencing by double-stranded RNA, Nature Reviews, 2001, vol. 2, 110-119 ) MacMilan Magazines Ltd. |
Hao, et al. (2004) Expression Analysis of the Human Testis-specific Serine/Threonine Kinase (TSSK) Homologues. A TSSK Member is Present in the Equatorial Segment of Human Sperm. Molecular Human Reproduction, vol. 10, No. 6 pp. 433-444. |
Harborth et al. Identification of Essential Genes in Cultured Mammalian Cells Using Small Interfering RNAs. (2001) J. Cell. Sci. 114:4557-4565). |
Ho, Potent Antisense Oligunucleotides to the Human Multidrug Resistance-1 mRNA are rationally selected by mapping RNA-accessible sites with Oligonucleotides Libraries. Nucleic Acids Research, vol. 24: 1901-1907 (1996). |
Holen, et al. (2002) Positional Effects of Shorts Interfering RNAs Targeting the Human Coagulation Trigger Tissue Factor. Nucleic Acids Research, vol. 30, No. 8, pp. 1757-1766. |
Human Neuropeptide Y Blast results, http://www.ncbi.nlm.nih.gov/blast, accessed on Mar. 18, 2008. |
Iliakis et al. (1990) Induction and Repair of DNA Strand Breaks in Radiation-resistant Cells Obtained by Transformation of Primary Rat Embryo Cells with the Oncogenes H-ras and v-myc. Cancer Research, 50, pp. 6575-6579. |
Ishibashi et al., A Novel Dual Specificity Phosphatase Induced by Setum Stimulation and Heat Shock, (1994) J. Biol. Chem. 47: 29897-2902). |
Jackson, et al., Expression profiling reveals off-target gene regulation by RNAi, Nature Biotechnology, vol. 21, pp. 635-637 (2003). |
Kalra, Central administration of antisense oligodeoxynucleotides to neuropeptide Y (NPY) mRNA reveals the critical role of newly synthesized NPY in regulation of LHRH release, Regulatory Peptides (1995) 215-220. |
Kasif, et al., A Computational Framework for Optimal Masking in the Synthesis of Oligonucleotide Microarrays, Nucleic Acids Research, 2002, vol. 30, No. 20, pp. 1-6. |
Khvorova, et al., Functional siRNAs and miRNAs Exhibit Strand Bias, Cell, Oct. 2003, vol. 115, pp. 209-216. |
Kretscmer-Kazemei, The Activity of siRNA in mammalian cells is related to structural target accessibility: a comparison with antisense oligonucleoitdes (2003) Nucleic Acids Research. vol. 31, No. 15, 4417-4424. |
Kumar, High-Throughput Selection of Effective RNAi Probes for Gene Silencing (2003). |
Laitala et al., Inhibition of Bone Resorption in Vitro by Antisense RNA and DNA Molecules Targeted against Carbonic Anhydrase II or Two Subuntis of Vacuolar H + ATPase, Journal of Clinical Investigation 1994, vol. 93, pp. 2311-2318. |
Lapidot-Lifson, et al. (1992) Cloning and Antisense Oligodeoxynucleotide Inhibition of a Human Homolog of cdc2 required in Hematopoiesis, Proc. Natl. Acad. Sci. vol. 89, pp. 579-583. |
Levenkova, Gene specific siRNA selector, Bioinofrmatics vol. 20, pp. 430-432 (2004). |
Lindgren, et al. (2002) Contribution of Known and Unknown Susceptibility Genes to Early-Onset Diabetes in Scandinavia, Diabetes vol. 51, 1609-1617. |
lshimi et al., Enhanced expression of MCM proteins in cancer cells derived from uterine cervix, 2003, European Journal of Biochemistry, vol. 270, pp. 1089-1101. |
Lu, et al., The Human AQP4 gene: Definition of the locus encoding two water channel polypeptides in brain, Proc. Natl Acad. Sci vol. 93, pp. 10908-10912 (Oct. 1996). |
Marathi, RAD1, a Human Structural Homolog of the Schizosaccharomyces pombe RAD1 Cell Cycle Checkpoint Gene, Genomcs 54, 344-347 (1998). |
Miller, V M et al., "Allele-specific silencing of dominant disease genes" Proceedings of the National Acadamy of Sciences of USA, vol. 100, No. 12, Jun. 10, 2003, pp. 7195-7200 , XP002276730. |
Miyagashi et al. (2003) Comparison of the Suppressive Effects of Antisense Oligonucleotides and siRNAs Directed Against the Same Targets in Mammalian Cells. Antisense and Nucleic Acid Drug Development 13:1-7. |
Murphy, et al. (2000) Synucleins are Developmentally Expressed, and Alpha-Synuclein Regulates the Size of the Presynaptic Vesicular Pool in Primary Hippocampal Neurons. The Journal of Neuroscience, vol. 20(9):3214-20. |
Naito, siDirect: highly effective, target specific siRNA design software for mammalian RNA interference, Nucleic Acids Research vol. 32, W124-129 (2004). |
NCBI Nucleotide Result for NM-002609, http://www.ncbi.nlm.nih.gov/sites/entrez, accessed on Jul. 14, 2008. |
NCBI Sequence Viewer v2.0 for NM-004438, http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=45439363, accessed on Jul. 3, 2008. |
Oishi, et al., Identification and Characterization of PKN Beta, a Novel lsoform of Protein Kinase PKN: Expression and Arachiodonic Acid Dependency Are Different from those of PKN alpha, 1999 Biochemical and Biophysical Research Communcation, 261, pp. 808-814. |
Olie et al, A Novel Antisense Oligonucleotide Targeting Survivin Expression Induces Apoptosis and Sensitizes Lung Cancer Cells to Chemotherapy, Cancer Research 2000, vol. 60: pp. 2805-2809. |
Pan, et al. (2005) Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation, The EMBO Journal, 24, 2104-2113. |
Promega siRNA Target Designer-Version 1.1, http:///www.promega.com/siRNADesigner/program/default.asp. (2003). |
Promega siRNA Target Designer-Version 1.51, http:///www.promega.com/siRNADesigner/program/default.asp. Accessed Jun. 24, 2008. |
Rabert et al. (1998) A Tetrodextrin-Resistant Voltage-gated Sodium Channel from Human Dorsal Root Ganglia, hPN3/SCN10A. Pain, 78, pp. 107-114. |
Rashid et al. Decreased plasma cholesterol and hypersensitivity to statins inmice lacking PCSK9, 2005 PNAS, vol. 102, pp. 5374-5379. |
Reynolds, et al., Rational siRNA design for RNA interference, Nature Biotechnology, Mar. 2004, vol. 22, No. 3, pp. 326-330. |
Ross, et al. (2001) Inhibition of Kirsten-ras Expression in Human Colorectal Cancer Using Rationally Selected Kirsten-ras Antisense Oligonucleotides, Molecular Cancer Therapeutics vol. 1, 29-41. |
Semizarov, Specificity of short interfereing RNA determined through gene expression signatures, Proceedings of the National Acadamy of Sciences USA vol. 100, pp. 6347-6352 (2003). |
Shi et al. (2001) Gremlin negatively Modulates BMP-4 Induction of Embryonic Mouse Lung Branching Morphogenesis. Am J Physiol Lung Cell Mol Physiol, 280, pp. L1030-L1039. |
siDesign Center for "gene name: src," http://www.dharmacon.com/DesignCenter/DesignCenterPage.aspx, accessed on May 13, 2008. |
siRNA Converter, http://web.archive.org/web/20020101-20021231re-/http://www.ambion.com/techlib/misc/siRNA-finder.html. Accessed Mar. 6, 2008. (siRNA Target Finder). |
siRNA Design for RNA Interference (RNAi) Experiments, http://web.archive.org/web/20010101000000-20021231235959/http://www.ambion.com/techlib/misc/siRNA-design.html. Accessed on Mar. 6, 2008. |
Sorensen, et al. (2003) Gene Silencing by Systemic Delivery of Synthetic siRNAs in Adult Mice, J. Mol. Biol. 327, 761-766. |
Tan et al. (2006) Functional Cooperation Between FACT and MCM Helicase Facilitates Initiation of Chromatin DNA Replication. The EMBO Journal, vol. 25, pp. 3975-3985. |
Truss, HuSide-the Human siRNA database: an open access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells, Nucleic Acids Research vol. 33, pp. D108-D111 (2005). |
Tsuji, et al. (2006) Essential Role of Phosphoorylation of MCM2 by Cdc7/Dbf4 in the Initiation of DNA Replication in Mammalian Cells. Molecular Biology of the Cell, vol. 17, pp. 4459-4472. |
Tuschl et al. (2003) The siRNA User Guide. 6 pages. |
Tuschl, et al. (2001) The siRNA User Guide. Max Planck Institute for Biophysical Chemistry, pp. 1, 3 and 5, http://www.mpibpc.gwdg.de/abteilungen/100/105/siRNAuserguide.pdf. |
Tuschl, Expanding small RNA interference (May 2002), Nature Biotechnology, vol. 20, pp. 446-448. |
Tusterman, et al., Dicers at RISC: The Mechanism of RNAi, Cell, Apr. 2004, vol. 117, pp. 1-4. |
Ui-Tei, et al., Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference, Nucleic Acids Research, Feb. 2004, vol. 32, No. 3, pp. 936-948. |
Vankayalapati, et al. (2003) Targeting Aurora2 Kinase in Oncogenesis: A Structural Bioinformatics Approach to Target Validation and Rational Drug Design. Molecular Cancer Therapeutics, v.2:283:94. |
Vickers et al., J. Biol. Chem., vol. 278, No. 9, pp. 7108-7118 (2003). * |
Vickers, Efficient Reduction of Target RNAs by Small Interfereing RNA and Rnase H-dependent Antisense Agents, J. Biol. Chem. vol. 278, No. 9, 7108-7118 (2003). |
Walton, et al. (1999) Prediction of Antisense Oligonucleotide Binding Affinity to a Structured RNA Target. Biotechnol. Bioengineering, v.65(1):1-9. (Abstract Only). |
Wang, et al., (2006) Dexamethasone Represses Signaling through the Mammalian Target of Rapamycin in Muscle Cells by Enhancing Expression of REDD1. The Journal of Biological Chemistry vol. 281, No. 51, pp. 39128-39134. |
Yao et al. (2000) Nature Cell Biology 2:484-491. |
Yuan, siRNA Selection Server: an automated siRNA oligonucleotide prediction server (2004). |
Zender et al., siRNA based strategies for inhibition of apoptotic pathways in vivo-analytical and therapeutic implications, Jan. 2004, vol. 9, pp. 51-54. |
Zhang, Physical and Functional lnterationc between Myeloid Cell Leukemia 1 Protein (MCL1) ad Fortilin, J. Biol. Chem. 37430-37438 (2002). |
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