|Publication number||US7695603 B2|
|Application number||US 11/200,369|
|Publication date||13 Apr 2010|
|Filing date||8 Aug 2005|
|Priority date||13 Jun 2001|
|Also published as||CA2450119A1, CA2450119C, EP1407330A2, EP1407330A4, EP1407330B1, US7597790, US7927477, US8685218, US8795493, US20020189947, US20020195344, US20040163957, US20070000784, US20090090174, US20100012497, US20110186157, WO2002101474A2, WO2002101474A3|
|Publication number||11200369, 200369, US 7695603 B2, US 7695603B2, US-B2-7695603, US7695603 B2, US7695603B2|
|Inventors||Phillip H. Paul, Don Wesley Arnold, Christopher G. Bailey|
|Original Assignee||Eksigent Technologies, Llc|
|Export Citation||BiBTeX, EndNote, RefMan|
|Patent Citations (104), Non-Patent Citations (66), Referenced by (4), Classifications (19), Legal Events (5)|
|External Links: USPTO, USPTO Assignment, Espacenet|
This application is a divisional of U.S. patent application Ser. No. 09/942,884, filed on Aug. 29, 2001, now abandoned which claims the benefit of U.S. Provisional Patent Application 60/298,147, filed Jun. 13, 2001, the entire contents of which are hereby incorporated herein by reference in their entirety.
The invention pertains to the fields of fluid handling and electroosmosis. More particularly, the invention pertains to electroosmotic flow controllers.
Flow controllers are used to manage the flow of fluids through conduits. Traditionally, control of fluid volume and or fluid composition by flow controllers is accomplished by combinations of pumps and valves. In some applications, flow controllers are used to control fluid flows on the order of many milliliters or more per minute, while in other applications, the fluid flow rates are orders of magnitude smaller. Prior art flow controllers tend to suffer from a variety of shortcomings. In high flow and in low flow applications, prior art flow controllers have difficulty in maintaining precise flow rates in the face of changing head pressures, such as those generated by pumping devices. In addition, mechanical feedback loops used to control flow rates through flow controllers often introduce additional imprecision and dead space into flow-controlled devices.
Microfluidic systems are playing an increasingly important role in developing advanced separation techniques needed for chemical analysis. Currently, chemical separations and purification represent severe bottlenecks in many biotechnology and drug discovery applications. Traditional scale separation systems are not compatible with the speed and small sample sizes typically required for high throughput analytical techniques. Analogous to transistors on a computer chip, microfluidic separation systems can be easily fabricated and run in multiple parallel arrays. The increased throughput and speed attained will be critical to allow separation technology to keep pace with the explosive growth of biotechnological advancement.
The recently completed sequencing of the human genome has tremendously increased our knowledge of the role genes play in disease. Translating this knowledge into improved medical diagnostics and treatments requires a much more thorough understanding of biological processes at the cellular and molecular levels. One promising approach for developing the necessary understanding involves analyzing the expression of cellular proteins in normal and disease states. Another approach involves generating protein-protein interaction maps that characterize binding interactions among different proteins to better understand the diverse biochemical signaling pathways underlying normal and pathological cell physiology.
These analytical tasks are in many respects more challenging than genome sequencing efforts. In contrast to DNA, which is chemically and structurally similar irrespective of nucleic acid sequence, proteins have enormous chemical diversity. Thus, different proteins may have different molecular weights, different electronic charges, different solubilities, etc. depending upon their amino acid composition and post-translational modifications.
Therefore, the reliable microseparation techniques developed for gene sequencing are inadequate for protein analysis. Moreover, the genome is a static entity. It does not significantly change over time and identical sequences of DNA are found in almost all of the cells in the body. In contrast, the complement of expressed proteins (i.e., the “proteome”) in, for example, a red blood cell, is very different than that expressed, e.g., in a neuron, or a skin cell, or a liver cell. Also, the proteome changes as a function of age and disease state. Further, considering alternative splicing possibilities, it has been estimated that there are 100 times more proteins present than the number of genes. Given this formidable task, it is clear that fast, reproducible and robust separation methods are needed to thoroughly characterize protein expression levels and interactions.
Currently, cell protein extracts are most often analyzed using 2 dimensional gel (“2D gel”) electrophoretic techniques, followed by one or more rounds of mass spectrometry (“MS”). Many problems exist with 2D gels, however. Inter- and intra-laboratory reproducibility is notoriously poor. The analysis is time consuming, the 2D separation typically taking 1-2 days. After this, proteins on the gel must be stained, identified, cut out, extracted from the gel matrix, destained and serially loaded into a mass spectrometer. A complete analysis for a single cell can take months or longer.
An alternative analytical approach involves the use of gradient liquid chromatography (“LC”) followed by MS. While this technique does not have the separation capacity of 2D gel electrophoresis, it is faster and more reproducible. The proteins found in the effluent of the separation column can be fed directly into a mass spectrometer. Commercially available high performance liquid chromatography (“HPLC”) systems for carrying out analytical separations often use separation columns having diameters on the order of 2-5 millimeters and run at flow rates of a few milliliters per minute (“ml/min”). Since the flow rate into the MS is on the scale of microliters per minute (“μl/min”) or less, most of the effluent from the HPLC, including that containing possibly valuable sample, is thrown away. Using a typical HPLC unit, these tandem liquid chromatography/mass spectroscopy (“LC/MS”) analyses take about an hour.
Increasing the throughput of HPLC separations can be accomplished in several ways. For example, separations can be carried out more quickly and multiple systems can be run in parallel. Given the high cost and large size of conventional HPLC systems, it is impractical to run more than a few systems in parallel. Also, the speed at which a separation can be done is limited by the large sample requirements of the system.
Microscale HPLC systems can, in theory, address both of these needs. Moreover, microscale systems offer other advantages such as reduced waste generation and low sample volume requirements. However, most currently available micro HPLC systems are simply large scale systems outfitted with flow reducers and dampers, and as such are not truly microscale flow devices. The macroscale high pressure pumps used in these systems are often unable to meter fluid flow with sufficient accuracy to generate reliable and rapid gradients, which are critical to reproducible HPLC analysis.
The present invention addresses these and other shortcomings of the prior art by providing electroosmotic flow controllers capable of providing precise flow rates in high and low flow applications using a combination of pressure-driven and electroosmotically-driven flows.
The present invention provides an electroosmotic flow controller capable of controlling fluid flow through a combination of electroosmotic and pressure-driven flows. The inventors have recognized that by adding an electroosmotic flow component to a pressure-driven flow component, one may effect rapid and accurate flow control over a wide range of flow rates. Devices embodying the invention may be made with few or no moving parts and are compatible with most solvents. They may be readily fabricated as true microscale devices. Devices embodying the present invention may be used to reliably and accurately correct for over pressurizations, pressure waves, and flow inaccuracies caused by the use of oversized pumps in microseparations. The devices can be driven by virtually any type of pressure generator, including high pressure syringe pumps, hand pumps, or air-driven systems.
Thus, in one aspect, the invention includes a flow controller that comprises a channel having a fluid inlet in liquid communication with a fluid source at pressure P1, a fluid outlet at pressure P2, wherein P2<P1, and a porous dielectric material disposed in the channel, a fluid contained within the channel, spaced electrodes in electrical communication with the fluid, a power supply in electrical communication with the electrodes for applying an electric potential to the spaced electrodes, whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates a pressure-driven flow component resulting from the P1−P2 pressure differential.
In another preferred embodiment, the flow controller further comprises in addition to the elements set forth above, a first flow element having a first flow element inlet in liquid communication at a first node at pressure PN with the fluid inlet and the fluid source, and a first flow element outlet at pressure P3, and the electroosmotically driven flow affects the proportion of fluid flowing through the channel and the first flow element.
In another preferred embodiment, the flow controller further comprises in addition to the elements set forth above, a second flow element having a second flow element inlet in liquid communication at the fluid source at pressure P1 with the fluid inlet and the fluid source, and a second flow element outlet in liquid communication at the node at pressure PN, and the second flow element affects the amount of fluid available at the node for passage through the channel and the first flow element.
In another preferred embodiment, the flow controller includes at least one sensor for monitoring at least one control signal, and a feedback control mechanism operatively connected to the sensor and the power supply, wherein the feedback control mechanism maintains the control signal within a predetermined range by modulating the electric potential applied by the power supply.
In another preferred embodiment, the sensor is selected from the group consisting of a pressure transducer, a flowmeter, a temperature sensor, a heat flux sensor, a displacement sensor, a load cell, a strain gauge, a conductivity sensor, a selective ion sensor, a pH sensor, a flow spectrophotometer, and a turbidity sensor.
In another preferred embodiment, the feedback control mechanism is programmable.
In still another preferred embodiment, the flow controller further comprises a second channel having a second fluid inlet in liquid communication with a second fluid source at pressure P4, a second fluid outlet at pressure P5, wherein P5<P4, and a second porous dielectric material disposed within the second channel, a second fluid contained within the second channel, second spaced electrodes in electrical communication with the second fluid, a second power supply in electrical communication with the second electrodes for applying a second electric potential to the second spaced electrodes, so that the second electric potential generates a second electroosmotically-driven flow component through the second channel that modulates a second pressure-driven flow component resulting from the P4−P5 pressure differential, a second flow element having a second flow element inlet in liquid communication at a second node at pressure PN2 with the second fluid inlet and the second fluid source, and a second flow element outlet in liquid communication at a third node with the first flow element outlet at pressure P3, so that the second electroosmotically driven flow affects the proportion of second fluid flowing through the second channel and the second flow element. One of ordinary skill will understand that the first and second power supplies need not be separate power supply units. In many instances it is preferable from a manufacturing economy standpoint that the first and second power supplies share components for generating power output. All that is intended by “second power supply” is a source of power output or potential difference that can be set independently from the first power supply. Thus, a single power supply unit with multiple, independently controllable voltage outputs would be considered a first and a second power supply according to the present invention.
In a further preferred embodiment, the flow controller further comprises a plurality of sensors that monitor a plurality of control signals and a feedback control mechanism operatively connected to the sensors, and to the power supplies so that the feedback control mechanism maintains the control signals within predetermined ranges by controlling the electric potentials applied by the power supplies.
In another preferred embodiment, the sensors are selected from the group consisting of a differential pressure transducer, a flowmeter, a temperature sensor, a heat flux sensor, a displacement sensor, a load cell, a strain gauge, a conductivity sensor, a selective ion sensor, a pH sensor, a flow spectrophotometer, and a turbidity sensor.
In a further preferred embodiment, the programmable feedback control mechanism maintains the sum of the fluid flowing through the first flow element outlet and the second flow element outlet within a predetermined range by monitoring a control signal originating at the third node, and adjusting the electric potentials applied by the power supplies.
In a preferred embodiment, the control signal originating at the third node is proportional to the pressure, P3.
In another preferred embodiment, the programmable feedback control mechanism maintains the relative amounts of fluid flowing from the first flow element outlet and the second flow element outlet within a predetermined range by monitoring control signals originating at the first node and the second node, and adjusting the electric potentials applied by the power supplies.
In yet another preferred embodiment, the control signals originating at the first node and the second node are proportional to the pressures PN and PN2.
In a preferred embodiment, the fluid contained within the channel comprises electrolytes.
In yet another preferred embodiment, the power supply is a variable power supply.
In another preferred embodiment, the pressure-driven and electroosmotically-driven flows are in the same direction.
In another preferred embodiment, the pressure-driven and electroosmotically-driven flows are in opposite directions.
In another preferred embodiment, the electrical communication between the spaced electrodes and the channel fluid is accomplished through a bridge.
In one preferred embodiment, the channel has a circular cross-section. In another embodiment, the channel cross-section is other than circular.
In another preferred embodiment, the channel comprises a fused silica capillary.
In another preferred embodiment, the porous dielectric material includes silica particles.
In yet another preferred embodiment, the silica particles have a diameter of between about 100 nm and 5 μm.
In a further preferred embodiment, the porous dielectric material includes porous dielectric materials fabricated by processes selected from the group consisting of lithographic patterning and etching, direct injection molding, sol-gel processing, and electroforming.
In an additional preferred embodiment, the porous dielectric material includes organic polymer materials.
It is yet another objective of the invention to provide methods for controlling fluid flow. In a preferred embodiment, the method comprises applying an electric potential to spaced electrodes in communication with a fluid contained within a porous dielectric disposed within a channel, the channel having a fluid inlet in liquid communication with a fluid source at pressure P1, and a fluid outlet at pressure P2, wherein P2<P1 and whereby the electric potential generates an electroosmotically-driven flow component through the channel that modulates a pressure-driven flow component resulting from the P1−P2 pressure differential.
In another preferred embodiment, the flow control method comprises applying an electric potential to spaced electrodes in communication with a fluid contained within a porous dielectric disposed within a channel, the channel having a fluid inlet in liquid communication at a node at pressure PN with a fluid source at pressure P1 and a first flow element inlet, and a fluid outlet at pressure P2, wherein P2<P1 and whereby the electric potential generates an electroosmotically-driven flow component through the channel that affects the proportion of fluid flowing through the channel and the first flow element.
Electroosmotic flow in open channels and in porous media is a well-know phenomena and has been the subject of many experimental and theoretical studies. When a liquid (e.g. water) is in contact with a dielectric solid (e.g. glass, silica, many plastics and ceramics) the natural electrochemistry of the interaction produces a thin layer of net charge density in the fluid that is localized to the liquid-solid interface. An electrical field applied so as to produce a component tangential to this interface will produce a Lorentz force on this net charge density. This net force will cause a motion of the net charge and this motion will be imparted by viscous action to the remaining neutral fluid. Thus in a channel packed with a porous dielectric material that is filled or saturated by an appropriate liquid, a potential difference ΔV applied end-to-end will produce what is known as an electroosmotic flow of the liquid. This electroosmotic flow may compete with or even dominate the flow that could be produced by application of a pressure difference across the same channel.
Electroosmotic flows may be generated using a wide variety of fluids and dielectric materials. The fluid should provide conditions that yield a high zeta potential with respect to the porous dielectric material. The fluid may be a pure fluid or a mixture of pure fluids that may have in addition some small concentration of a conducting species such as various ions. Preferably, the pure fluids should have high dielectric constant (between about 5 and 100 relative units), low dynamic viscosity (between about 0.1 and 2 centipoise) and low conductivity (between about 10−4 and 10−14 mho/m). Additives are preferably introduced to define or control the pH and ionic strength of the fluid. Additives should be of a kind and of a concentration to completely dissolve in the fluid. The kind and concentration of these additives preferably are chosen so as to enhance or optimize the zeta potential under the conditions imposed by the size of the pores in the porous dielectric medium.
Suitable pure fluids include by way of example, but not limitation: distilled and/or deionized water, cyclic carbonates, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 1-pentanol, 1-hexanol, 1-heptanol, benzyl-alcohol, nitromethane, nitrobenzene, butanone, dimethoxymethane, dimethylacetamide, dioxane, p-dioxane, acetonitrile, formamide, tetrahydrofuran, dimethyl formamide, acetone, acetic acid, triethylamine, dichloromethane, ethylene glycol, dimethylsulfoxide, ammonium acetate.
The zeta potential may be thought of as a property of a fluid-solid interface. It is desirable that the magnitude of this zeta potential be in the range of about unity to 150 mV. The zeta potential may be either positive or negative in sign. It is known that the sign and magnitude of the zeta potential are dependent on the dielectric constant of the fluid, the pH of the fluid, on the ionic strength of the fluid and on the type of ions in the fluid. To yield a zeta potential, generally, the surface of the porous dielectric material exhibits acidic or basic sites that become ionized in the presence of the fluid. These ionizable surface sites may be native to the material or may be the result of adsorption of some species onto the surface material.
Native ionizable materials include by way of example, but not limitation: silica, which exhibits acidic surface sites, alumina (amphoteric) which can exhibit basic or acidic surface sites, Nylon (zwitterionic) which exhibits both acidic (carboxyl) and basic (amine) surface sites. The sign of the zeta potential is the same as the sign of the net surface charge.
As an example of adsorption leading to surface charge, consider admixtures of polyethylene or polypropylene with ionic surfactants. Polyethylene and polypropylene are non-polar polymers having no native ionizable sites. In an aqueous solution containing certain ionic surfactants (e.g. sodium dodecyl sulfate), the hydrophobic tail of the surfactant adsorbs to the polymer. The charged end of the surfactant then appears as a charge site on the surface.
The degree of ionization of the surface sites depends on the pH of the fluid. In most cases there is a pH at which the surface is net neutral and hence the zeta potential is zero. The zeta potential reaches a maximum value for pH values well-above (for acidic surface sites) or pH values well below (for basic surface sites) the pH value at which the surface is net neutral. Ionizable surface sites can be added to a material by chemical reaction or grafting, or induced by creation of reactive surface chemistry or creation of defects via plasma or radiation treatment.
The host dielectric material is selected for properties of: high zeta potential, sign of the zeta potential, insolubility and stability in the fluid with additives, low electrical conductivity, and sufficient mechanical strength.
Examples of suitable oxide materials include: silica, alumina, titania, zirconia, cerium oxide, lanthanum oxide, yttrium oxide, hafnium oxide, magnesium oxide, tantalum oxide. These oxides may be amorphous or glassy or crystalline and may be combined in mixtures having other minor oxide components.
Examples of suitable glass materials include: crown or float or boro-silicate glasses, lanthanum or flint or dense flint glasses, Pyrex™.
Examples of suitable nitride materials include: silicon nitride, boron nitride, aluminum nitride.
Examples of suitable polymers include: Nafion™ (Dupont Trade name, a sulfonated PTFE), polysulfone, polyethersulfone, cellulose acetate, mixed cellulose esters, polycarbonate, polyacrylonitrile, polyvinylidene fluoride, polyamide (Nylon), silicone elastomers, polymethacrylate, and nitro-cellulose (also called collodion).
Other classes of suitable materials include certain semiconductors, carbides (e.g. titanium carbide) and silicides (e.g. germanium silicide).
Ionic species in the fluid are termed counterions (ions that have a charge sign opposite the sign of the zeta potential) and coions (ions that have a charge sign the same as the sign of the zeta potential). It is the net excess of surface charge-balancing counterions near the surface/fluid interface that determines the zeta potential. Increasing the concentration of counterions in the bulk fluid tends to shield the surface charge and thus reduces the magnitude of the zeta potential. For example, consider silica as the dielectric material exposed to water at pH 7 as the pure fluid and KCl as an additive. The zeta potential for this system is measured to be negative with magnitudes of: 120 mV, 100 mV, 70 mV and 30 mV for KCl concentrations of 0.1, 1, 10 and 100 millimolar, respectively. The valence of the counterion may also have a pronounced effect on the character of the zeta potential. Polyvalent (i.e. multiply charged) counterions may bind to the surface sites thus changing the pH of zero net charge (i.e. the “isoelectric pH”). For example: silica in the presence of a singly valent counterion (e.g. Na+) displays an isoelectric pH of about 2.8. Whereas silica in the presence of a bivalent counterion (e.g. Ca2+ or Ba2+) displays an isoelectric pH in the range of 6 to 7. In this regard, the transport fluid preferably is selected or purified so as to be substantially free of polyvalent counterions.
Ionic additives to the fluid may be broken into two general classes: those that fully ionize (e.g. salts, strong acids and strong bases) and those that partially ionize. The former class is often employed primarily to establish the ionic strength of the fluid. The latter class is often employed primarily to buffer the fluid and thus establish and maintain the pH of the fluid. The two classes often are used in conjunction. It is important to note that many but not all buffering species can exist in polyvalent states (e.g. formate exists as neutral or singly charged whereas phosphate exists as neutral, singly, doubly and triply charged). Thus the choice of a buffering compound must be made in view of the issue of polyvalent counterions discussed above.
Examples of ionic and buffering additives include but are not limited to: alkali-halide salts, mineral acids and bases, organic acids and bases, phosphates, borates, acetates, citrates, malates, formates, carbonates, chlorates, nitrates, sulfates and sulfites, nitrates and nitrites, ammonium-, methylammonium-, ethylammonium-, propylammonium-salts, BIS, MES, TRIS, TES, HEPES, TEA.
Certain compounds (sometimes termed anti-static agents) are known to alter or eliminate the zeta potential. For example special agents are added to hydrocarbon fuels to eliminate zeta potentials and thus prevent static buildup during pumping and transport. As a further example, special agents are added to shampoos and conditioners again to eliminate the zeta potential and prevent static buildup. Certain surfactants represent one class of these agents. In this regard the transport fluid is selected or purified so as to be substantially free of agents that degrade or eliminate the zeta potential. As examples: addition of small quantities of the surfactant SDS (sodium dodecyl sulfate) is known to increase the zeta potential of silica in aqueous solutions. Whereas the effect of the surfactant CTAB (cetyl trimethylammonium bromide) on silica in water is to reduce the zeta potential upon addition at low concentrations, to a value near zero as the concentration is increased, and to reverse the sign of the zeta potential at even higher concentrations. Addition of polyamines are also known to reduce or reverse the zeta potential of silica. Surface modification properties of surfactants are reviewed by M. J. Rosen, ‘Adsorption of surface-active agents at interfaces: the electrical double layer,’ Chapter II in, Surfactants and Interaction Phenomena (Wiley, N.Y., 1986), pp. 33-107.
The region of net charge in the fluid and adjacent to the dielectric surface extends some distance into the fluid. The one-on-e (1/e) thickness of this layer is approximately the Debye length in the bulk fluid. The Debye length at a temperature of 20° C. has a value of 0.048 nm times the square root of ratio of the fluid dielectric constant to the fluid ionic strength (the later taken in units of mols/liter). For one millimolar KCl in water the Debye length is about 9.6 nm.
This Debye length scale can be altered by changing the ionic strength of the fluid and is preferably less than about one-fifth the characteristic pore size of the porous dielectric medium. For Debye lengths greater than about one-fifth the characteristic pore size, the charged layers on opposing walls of the pore begin to substantially merge having the effect of reducing the apparent zeta potential. For quantitative determination of the degree of double layer overlap the characteristic pore size, Dpore, is preferably taken as defined by D. L. Johnson and P. N. Sen, Phys. Rev. B 37, 3502-3510 (1988); D. L. Johnson, J. Koplick and J. M. Schwartz, Phys. Rev. Lett. 57, 2564-2567 (1986); and D. L. Johnson, J. Koplick and R. Dashen, J. Fluid Mech. 176, 379-392 (1987). This definition of Dpore produces a strong weighting in favor of the larger through-pores in a porous medium.
The flowrate through a porous medium is given by Darcy's law:
Q=−k D ΔPA/L
Here ΔP is the applied pressure difference driving the flow, A and L are the geometrical cross sectional area and thickness of the porous medium, respectively, kD is the flow or Darcy permeability of the medium. Using the definition of Dpore given above, the Darcy permeability is given by:
k D =D pore 2 M/F
where M is termed the ‘pore geometry number’ and F is the ‘formation factor’ of the porous media. The formation factor, F, may be related to more common descriptors of porous media via F=τ2/φ where τ is termed the tortuoisty and φ is the connected porosity of the solid. The connected porosity is the wetted volume fraction that represents the through-connected pores and excludes dead-ended pores. Each of these descriptors may be determined using any of the methods well known in the art.
The effect of charge-layer overlap in simple geometries (e.g. slit or circular pores) has been studied theoretically. See, e.g., C. L. Rice and R. Whitehead, ‘Electrokinetic flow in a narrow cylindrical pore,’ J. Phys. Chem. 69 pp. 4017-4024 (1965); and D. Burgreen and F. R. Nakache ‘Electrokinetic flow in ultrafine capillary slit,’ J. Phys. Chem. 68 pp. 1084-1091 (1964). The conclusions of these studies can be applied analogously to a general porous medium through the use of Dpore as defined above.
Pores in a porous material vary in size along the length and a variety of pore sizes may be present. Thus a general porous material, saturated with a fluid at some given ionic strength, may have some subset of pores that contain substantially overlapped regions of net charge (here termed ‘nanopores’) with the balance of the pores containing some amount of core fluid that is free of charge-layer overlap (here termed ‘regular’ pores). All of the pores will transport current hence ionic species, but the nanopores will transport flow at a greatly reduced rate compared to the regular pores. An object of the present invention is to apply a current so as to create a flow with minimal alteration of fluid ionic composition. The presence of nanopores reduces the efficiency of this process and may also lead to substantial and performance-degrading ionic strength, composition, and pH gradients across the porous element.
Experimental and theoretical studies of a wide range of porous media suggest that the pore geometry number falls into the range 1/32<M< 1/16, whereas the formation factor, F, is simply greater than or equal to the inverse of the connected porosity. No particular conditions are placed on the values of M or F for practice of the present invention, except to the extent that both values remain finite (essentially a condition that the porous material is indeed porous and connected end-to-end). Note that the topology properties of a porous material are important design parameters and also that optimization of a particular design may involve the specific choice of porous materials having particular values for these parameters.
Porous materials may be fabricated by a wide variety of methods, examples include but are not limited to the following:
The porous materials may be fabricated in-channel or may be fabricated, possibly machined or cut, and then inserted or sealed into the channel. The surface properties may be altered before or after placement within a channel.
The sign and magnitude of the zeta potential can be altered or enhanced by modification of the surface or bulk chemistry of the porous material as described above. Modification of surface chemistry is generally done by reaction with sites (e.g. silanol, hydroxyl, amine) that are present on the native material. Modification of the bulk chemistry is generally done by synthesis of a material that directly incorporates ionizable sites. Examples include but are not limited to the following:
The porous dielectric material is contained in a fluid-impermeable ‘channel’ having a fluid inlet and outlet and spaced electrodes for applying a potential difference to the fluid. Channel materials are selected to meet requirements for mechanical strength, dielectric breakdown strength, transport fluid and fluid additive compatibility, and the capacity to retain the porous dielectric material. The geometry of the channel covers the entire range from long in length and small cross section to short in length and large cross section. An example of the former geometry is a channel that may be a capillary tube or a covered microchannel formed in a substrate having cross sectional shapes including round to rectangular to rectangular with sloped or curved sides. This channel may be formed by any of the means known in the art. An example of the later geometry is a large diameter and thin porous membrane as sandwiched between two electrodes
The choice of pore size, topology numbers and physical geometry (e.g. porous element thickness and cross-sectional area) are particular to a given application. This then drives the needs for ionic strength and buffering capacity. In general, the following considerations may be taken into account for practicing preferred embodiments of the present invention.
The principles and operation of the invention will now be described by reference to the following figures which are intended to serve as illustrative embodiments but not to limit the scope of the invention.
It is well-known to one of skill in the art that application of an electrical potential to a fluid via electrodes 105, 106 in that fluid can generate a current through the fluid, and that gas will be generated at the electrodes 105, 106 via electrolysis of the fluid. It is further appreciated that gas generation within a closed fluid channel may be undesirable. Thus, as shown in
The flow rate may be written as: Q=(νΔV−κΔP)A/LF. This relation is a well known combination of Darcy's law for pressure driven flow and the Helmholtz-Smoluchowski relation as adapted for electroosmotic flow in porous media. Here ν is the effective electroosmotic mobility, κ is the Darcy permeability of the porous media as divided by the dynamic viscosity of the liquid, and F is the above-described the formation factor. The effective mobility may be written as ν=∈ζ(1−ξ)/μ where ∈ and μ are the dielectric permittivity and dynamic viscosity of the fluid, respectively, ζ is the zeta potential and ξ is a factor that provides for the effect of overlapping net charge layers (i.e. a reduction of the apparent zeta potential under conditions that the thickness of the charge layers becomes on the order of the size of the pores in the media). The zeta potential, hence the electroosmotic mobility, may be signed positive or negative depending on the nature of the fluid and the dielectric material (e.g. for a porous dielectric material 104 composed of TiO2 saturated with an aqueous solution, the zeta potential will have a positive sign at low pH and a negative sign at high pH and will be negligibly small at the material iso-electric point which for TiO2 is at about pH 6.2).
The present invention employs a combination of pressure- and electroosmotically-driven flows in a channel 100 filled with a porous dielectric material 104. The applied potential preferably is selected to yield an electroosmotic flow in the same direction as the pressure-driven flow (e.g. for TiO2 at high pH, hence a negative zeta potential hence a negative electroosmotic mobility, the potential would be applied with the negative terminal downstream with respect to direction of the pressure-driven flow). In this configuration the maximum flow rate through the channel 100 will be given by the flow rate equation above and only limited by the magnitude of the potential applied, whereas the minimum flow rate will be for purely pressure-driven flow, that is with ΔV=0, hence Q=−κΔPA/LF. Thus the combination of pressure- and electroosmotically-driven flow in a channel 100 filled with a porous dielectric material 104 provides a voltage-controlled means to vary the flow rate through that channel. In effect, flow control is provided by varying the degree of electroosmotic ‘assist’ to the pressure-driven flow through the channel. As is explained in greater detail with respect to other preferred embodiments described below, sensors may be used to monitor parameters such as pressure, flow rate, etc. at one or more points in the flow controller system. Signals arising from these sensors may be used in a sense and control or servo loop to maintain the signal within a predetermined range by adjusting the voltage outputted by the power supply in response to deviations between the signal and a predetermined set point.
The system of
Consider the gauge pressure P2 to be zero, that is, ambient pressure. However, this embodiment is not limited to this condition which is provided purely for illustration of this application. The flow rate Q3 through flow element 201 is given by:
Q 3 =k 3(k 1 P 1(1−x)−(k 1 +k 2)P 3)/(k 1 +k 2 +k 3)
Here we define the quantities: k≡κA/LF and x≡(ν2/κ2)k2ΔV/k1P1. Those skilled in the art will note that the variable k can be considered effectively as the above-mentioned pressure-driven flow resistance parameter for each flow element or channel. Thus for ΔV=0, hence x=0, the flow rate through flow element 201 has a value of:
Q 3 =k 3(k 1 P 1−(k 1 +k 2)P 3)/(k 1 +k 2 +k 3)
whereas this flow rate Q3 (i.e. the flow rate through flow element 201) is zero when:
x=1−(k 1 +k 2)P 3 /k 1 P 1
hence this flow rate is zero when the potential is set to a value of
ΔV=(k 1 P 1−(k 1 +k 2)P 3)(κ2/ν2)
The flow rate Q3 through flow element 201 can be made negative (i.e. the flow direction through flow element reversed) by the application of even higher values of the potential.
The Darcy flow resistance for flow element 205 is selected based upon on the desired range of flow rates through flow element 201 and the electroosmotic flow rate that is achieved when a maximum voltage is supplied across channel 100 by power source 107. For example, if one desires the ability to halt flow through element 201, Pnode must be equal to P3. The pressure at node 202 is given by: Pnode=(k1P1(1−x)+k3P3)/(k1+k2+k3). Thus, the relative resistances of flow element 205 and channel 100 should be designed to allow electroosmotic flow through channel 100 to be equal to the pressure driven flow through flow element 205. Appropriate selections of relative flow resistances for channel 100, flow element 205, and flow element 201 for a particular application are readily determined using the equations provided above by those skilled in the art.
The control so achievable is limited by the condition that the pressure at P1 remains sufficiently high to supply the required flow rate. This type of feedback control may be accomplished by any of the means that are well-know in the art, for example: observing a pressure or flow reading at node 202 by use of sensor 301 and manually adjusting the potential applied by power source 107; measuring the pressure or flow at node 202 with sensor 301 and supplying this measurement to an analog electronic (or mechanical) servo loop controller 302 driving an electronically (or mechanically) adjustable power supply 107; measuring the pressure or flow at node 202 with a sensor 301 connected to a computer and using the computer to adjust power supply 107, optionally, with higher order corrections applied (e.g. corrections for fluid or sensor temperature variations) in light of other data being supplied to the computer.
One of skill will readily appreciate that multiple devices such as those illustrated in
The servo loop described above may employ a variety of control inputs and action outputs. By way of example, but not limitation, with the object of providing a constant flow rate Q3 through flow element 201 the input to the servo loop is taken as, e.g., the differential pressure across flow element 201 (see
With the object to supply a flow rate of liquid used for heat transfer and by this the control of a temperature or heat flux as a result of the flow of liquid through flow element 201 sensors 301 and 311 (as shown in
With the object of applying a mechanical force or displacement through the application of fluid pressure to a bellows 501 (see
The designs represented in
The system illustrated in
In the example of
The objective in this illustration is to provide constant flow rate to the column 617 while providing a programmed variation in fluid composition. The flow rates of fluids A and B from sources 102 and 602 are independently measured and servo-controlled by two sense-and-control loops involving elements 301, 302, 107, 613, 614, 615, 607 that are provided with set-point inputs 618, 619. The programmed variation in fluid composition may be in the form of a series of step changes, or in the form of a continuous ramp (i.e. a gradient) or any of the other forms known in the separation arts. In applications requiring more than two sources of fluid, attendant flow controllers and servo loops may be combined to provide for more complicated or broad ranging fluid composition variations. Such configurations can be run in parallel from common sources of fluids to be able to perform multiple separations in parallel.
For the purpose of this illustration, sample injection through a sample loop 626 connected to a sample injector valve 616 at the head of the separation column 617 is taken to be performed by any of the means known in the HPLC arts (e.g. by a specialized sample injection valve e.g. 616, or by electroosmotic/electrophoretic injection through a porous media). For the purpose of this illustration, the end-use of the separation is taken to be any of the end-uses know in the HPLC arts (e.g. such as analyte detection by a detector 620 that measures, e.g., laser-induced fluorescence, optical absorption, refractive index or electrochemical potential; collection of the separation components; input to a mass spectrometer or ICP or NMR spectrometers; input to a next stage of separation by HPLC or LC or electrochromatographies; or preparative HPLC).
The data shown in
The data shown in
Since the set point of the flow controller can be changed to almost any value less than the driving pressure, two or more flow controllers may be combined to deliver fast, accurate, and reproducible gradients for use in microscale separations. A single pressure source can be used to drive all of the different fluids used in the gradient. Since the flow controller is a microscale device, it is compatible with being operated in a multiple parallel configuration.
As noted above, the presence of a current-carrying electrode in a closed channel may produce undesirable side effects. Bridges provide one method of removing the electrode from the channel but still providing current. As also noted above, the zeta potential is a function of fluid composition and pH. As such any given flow control porous element may operate under some limited range of fluid conditions.
For example, the material 104 in channel 100 may be silica with a nominal iso-electric point of pH 3 and the material 1104 in channel 1100 may be alumina with an iso-electric point of pH 9.2. As a further example, the material 104 may be modified to display a sulfonic acid group (nominal iso-electric point of pH 1.5) and the media 1104 may be modified to support a quaternary amine (nominal iso-electric point higher than pH 14). For a fluid with a pH between the iso-electric points of the two materials the electroosmotic flow through one channel will be towards the supply anode and the electroosmotic flow through the other channel will be towards the supply cathode. This then provides flow hence flow control over a wider range of pH conditions than could be supported using a single channel and at the same time removes the current-carrying electrodes 105, 106 from the closed channels 100, 1100.
As a specific example consider the materials 104, 1104 in channels 100 and 1100 to be silica and alumina, respectively. With fluid having pH 3 channel 100 filled with silica has a negligible zeta potential and thus does not provide electroosmotic flow, but still carries current. Channel 1100 filled with alumina has a high positive zeta potential with fluid having pH 3 and thus provides the electroosmotic flow (from the common junction 202 of the channels towards the supply anode) needed for flow control. With fluid having pH 9 the roles are reversed, the silica displays a high negative zeta potential whereas the alumina has a negligible zeta potential, thus the electroosmotic flow is through channel 100 filled with silica (from the common junction 202 of the channels towards the supply cathode). For a fluid having a pH between pH 3 and pH 9 channels 100 and 1100 both supply some degree of electroosmotic flow and thus contribute to the ability to achieve flow control.
The foregoing description and figures are intended to illustrate but not limit the scope of the invention. Variations of what has been described will be apparent to those skilled in the art and are encompassed by invention described above. All references to patents, patent applications, and other publications are herein incorporated by reference in their entirety for any and all purposes.
|Cited Patent||Filing date||Publication date||Applicant||Title|
|US2615940||25 Oct 1949||28 Oct 1952||Milton Williams||Electrokinetic transducing method and apparatus|
|US2644900||27 Nov 1951||7 Jul 1953||Hardway Jr Edward V||Electrokinetic device|
|US2644902||27 Nov 1951||7 Jul 1953||Jr Edward V Hardway||Electrokinetic device and electrode arrangement therefor|
|US2661430||27 Nov 1951||1 Dec 1953||Jr Edward V Hardway||Electrokinetic measuring instrument|
|US2995714||13 Jul 1955||8 Aug 1961||Kenneth W Hannah||Electrolytic oscillator|
|US3143691||28 Nov 1958||4 Aug 1964||Union Carbide Corp||Electro-osmotic cell|
|US3209255||22 Apr 1960||28 Sep 1965||Union Carbide Corp||Electro-osmotic current integrator with capillary tube indicator|
|US3427978||24 Jan 1968||18 Feb 1969||Electro Dynamics Inc||Electro-hydraulic transducer|
|US3544237||19 Dec 1968||1 Dec 1970||Dornier System Gmbh||Hydraulic regulating device|
|US3682239||25 Feb 1971||8 Aug 1972||Momtaz M Abu Romia||Electrokinetic heat pipe|
|US3921041||24 Feb 1975||18 Nov 1975||American Radionic||Dual capacitor|
|US3923426||15 Aug 1974||2 Dec 1975||Alza Corp||Electroosmotic pump and fluid dispenser including same|
|US4347131||28 Apr 1981||31 Aug 1982||Robert Brownlee||Liquid chromatographic pump module|
|US4638444||17 Feb 1983||20 Jan 1987||Chemical Data Systems, Inc.||Microprocessor-controlled back-pressure system for small volume chemical analysis applications|
|US4681678||10 Oct 1986||21 Jul 1987||Combustion Engineering, Inc.||Sample dilution system for supercritical fluid chromatography|
|US4684465||10 Oct 1986||4 Aug 1987||Combustion Engineering, Inc.||Supercritical fluid chromatograph with pneumatically controlled pump|
|US4767279||2 Jun 1987||30 Aug 1988||Millipore Corporation||Fluid composition and volumetric delivery control|
|US4810392||17 Dec 1987||7 Mar 1989||W. R. Grace & Co.||Sample dispensing system for liquid chromatography|
|US4921041||15 Jun 1988||1 May 1990||Actronics Kabushiki Kaisha||Structure of a heat pipe|
|US5032264||5 Feb 1990||16 Jul 1991||Geiger Industries And Design Group Ltd.||Catch flow restrictor with opening calibrated to flow and head chart|
|US5040126||15 Oct 1985||13 Aug 1991||Isco, Inc.||Method for predicting steady-state conditions|
|US5219020||15 Aug 1991||15 Jun 1993||Actronics Kabushiki Kaisha||Structure of micro-heat pipe|
|US5249929||13 Aug 1990||5 Oct 1993||The Dow Chemical Company||Liquid chromatographic pump|
|US5302264 *||2 Sep 1992||12 Apr 1994||Scientronix, Inc.||Capillary eletrophoresis method and apparatus|
|US5312233||25 Feb 1993||17 May 1994||Ivek Corporation||Linear liquid dispensing pump for dispensing liquid in nanoliter volumes|
|US5418079||21 Jun 1994||23 May 1995||Sulzer Innotec Ag||Axially symmetric fuel cell battery|
|US5429728||23 Sep 1993||4 Jul 1995||Hewlett-Packard Company||Electroosmotic flow control using back pressure in capillary electrophoresis|
|US5482608||19 Jan 1993||9 Jan 1996||Hewlett Packard Company||Capillary electrophoresis flow control system|
|US5573651||31 May 1995||12 Nov 1996||The Dow Chemical Company||Apparatus and method for flow injection analysis|
|US5630706||31 Aug 1993||20 May 1997||Yang; Frank J.||Multichannel pump apparatus with microflow rate capability|
|US5664938||23 Feb 1995||9 Sep 1997||Yang; Frank Jiann-Fu||Mixing apparatus for microflow gradient pumping|
|US5670707||1 Nov 1996||23 Sep 1997||Varian Associates, Inc.||Calibration method for a chromatography column|
|US5777213||21 Nov 1996||7 Jul 1998||Tfc Corporation||Preparative liquid chromatography apparatus|
|US5797719||30 Oct 1996||25 Aug 1998||Supercritical Fluid Technologies, Inc.||Precision high pressure control assembly|
|US5814742||11 Oct 1996||29 Sep 1998||L C Packings, Nederland B.V.||Fully automated micro-autosampler for micro, capillary and nano high performance liquid chromatography|
|US5858193||9 Nov 1995||12 Jan 1999||Sarnoff Corporation||Electrokinetic pumping|
|US5888050||25 Aug 1998||30 Mar 1999||Supercritical Fluid Technologies, Inc.||Precision high pressure control assembly|
|US5915401||6 Sep 1996||29 Jun 1999||Thomson-Csf||Servocontrol process for a servovalve which can be servocontrolled in terms of flow rate and pressure|
|US5942093||18 Jun 1997||24 Aug 1999||Sandia Corporation||Electro-osmotically driven liquid delivery method and apparatus|
|US5961800||8 May 1997||5 Oct 1999||Sarnoff Corporation||Indirect electrode-based pumps|
|US5997746||29 May 1998||7 Dec 1999||New Objective Inc.||Evaporative packing of capillary columns|
|US6004443||13 Aug 1997||21 Dec 1999||Rhodes; Percy H.||Chromatography-format fluid electrophoresis|
|US6012902||25 Sep 1997||11 Jan 2000||Caliper Technologies Corp.||Micropump|
|US6013164||25 Jun 1997||11 Jan 2000||Sandia Corporation||Electokinetic high pressure hydraulic system|
|US6019882 *||7 Apr 1998||1 Feb 2000||Sandia Corporation||Electrokinetic high pressure hydraulic system|
|US6045933||27 Jan 1998||4 Apr 2000||Honda Giken Kogyo Kabushiki Kaisha||Method of supplying fuel gas to a fuel cell|
|US6068243||5 Jan 1998||30 May 2000||A & B Plastics, Inc.||Self-locking, adjustable-width slat for chain link fences|
|US6068767||29 Oct 1998||30 May 2000||Sandia Corporation||Device to improve detection in electro-chromatography|
|US6086243||1 Oct 1998||11 Jul 2000||Sandia Corporation||Electrokinetic micro-fluid mixer|
|US6106685||24 Dec 1997||22 Aug 2000||Sarnoff Corporation||Electrode combinations for pumping fluids|
|US6139734||20 Oct 1998||31 Oct 2000||University Of Virginia Patent Foundation||Apparatus for structural characterization of biological moieties through HPLC separation|
|US6149787 *||14 Oct 1998||21 Nov 2000||Caliper Technologies Corp.||External material accession systems and methods|
|US6167910||14 Jan 1999||2 Jan 2001||Caliper Technologies Corp.||Multi-layer microfluidic devices|
|US6221332||5 Aug 1998||24 Apr 2001||Microfluidics International Corp.||Multiple stream high pressure mixer/reactor|
|US6224728||13 Aug 1999||1 May 2001||Sandia Corporation||Valve for fluid control|
|US6255551||4 Jun 1999||3 Jul 2001||General Electric Company||Method and system for treating contaminated media|
|US6277257||18 Mar 1999||21 Aug 2001||Sandia Corporation||Electrokinetic high pressure hydraulic system|
|US6280967||5 Aug 1999||28 Aug 2001||Axiom Biotechnologies, Inc.||Cell flow apparatus and method for real-time of cellular responses|
|US6287440||18 Jun 1999||11 Sep 2001||Sandia Corporation||Method for eliminating gas blocking in electrokinetic pumping systems|
|US6289914||16 Aug 2000||18 Sep 2001||Novartis Ag||Microflow splitter|
|US6290909||13 Apr 2000||18 Sep 2001||Sandia Corporation||Sample injector for high pressure liquid chromatography|
|US6299767||29 Oct 1999||9 Oct 2001||Waters Investments Limited||High pressure capillary liquid chromatography solvent delivery system|
|US6315905||24 Jan 2000||13 Nov 2001||University Of Virginia Patent Foundation||Method for structural characterization of biological moieties through HPLC separation|
|US6319410||8 Mar 2000||20 Nov 2001||Isco, Inc.||Apparatus and method for super critical fluid extraction|
|US6386050||21 Dec 1999||14 May 2002||Agilent Technologies, Inc.||Non-invasive fluid flow sensing based on injected heat tracers and indirect temperature monitoring|
|US6402946||26 Oct 2000||11 Jun 2002||Bruker Analytik Gmbh||Device for feeding a chromatography flow|
|US6404193||9 Apr 2001||11 Jun 2002||Waters Investments Limited||Solvent susceptibility compensation for coupled LC-NMR|
|US6406605||8 May 2000||18 Jun 2002||Ysi Incorporated||Electroosmotic flow controlled microfluidic devices|
|US6416642||5 Feb 1999||9 Jul 2002||Caliper Technologies Corp.||Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection|
|US6428666||22 Feb 1999||6 Aug 2002||Sandia National Laboratories||Electrokinetic concentration of charged molecules|
|US6460420||13 Apr 2000||8 Oct 2002||Sandia National Laboratories||Flowmeter for pressure-driven chromatography systems|
|US6477410||31 May 2000||5 Nov 2002||Biophoretic Therapeutic Systems, Llc||Electrokinetic delivery of medicaments|
|US6616823||15 Feb 2001||9 Sep 2003||Caliper Technologies Corp.||Systems for monitoring and controlling fluid flow rates in microfluidic systems|
|US6619311||19 Apr 2002||16 Sep 2003||Nanostream, Inc.||Microfluidic regulating device|
|US6719535||31 Jan 2002||13 Apr 2004||Eksigent Technologies, Llc||Variable potential electrokinetic device|
|US6766817||25 Feb 2002||27 Jul 2004||Tubarc Technologies, Llc||Fluid conduction utilizing a reversible unsaturated siphon with tubarc porosity action|
|US20010008212||28 Feb 2001||19 Jul 2001||Shepodd Timothy J.||Castable three-dimensional stationary phase for electric field-driven applications|
|US20020017484||14 Sep 2001||14 Feb 2002||Waters Investments Limited||High pressure capillary liquid chromatography solvent delivery stystem|
|US20020022802||13 Jun 2001||21 Feb 2002||Simpson Frank B.||Wrap spring clutch syringe ram and frit mixer|
|US20020070116||13 Dec 2000||13 Jun 2002||Tihiro Ohkawa||Ferroelectric electro-osmotic pump|
|US20020072126||3 Oct 2001||13 Jun 2002||Dionex Corporation||Method and system for peak parking in liquid chromatography-mass spectrometer (LC-MS) analysis|
|US20020076598||15 Dec 2000||20 Jun 2002||Motorola, Inc.||Direct methanol fuel cell including integrated flow field and method of fabrication|
|US20020125134||24 Jan 2002||12 Sep 2002||Santiago Juan G.||Electrokinetic instability micromixer|
|US20020189947||29 Aug 2001||19 Dec 2002||Eksigent Technologies Llp||Electroosmotic flow controller|
|US20020195344||24 May 2002||26 Dec 2002||Neyer David W.||Combined electroosmotic and pressure driven flow system|
|US20030052007||17 Sep 2002||20 Mar 2003||Paul Phillip H.||Precision flow control system|
|US20030138678||18 Feb 2003||24 Jul 2003||Walter Preidel||Method for mixing fuel in water, associated device, and implementation of the mixing device|
|US20030190514||4 Dec 2002||9 Oct 2003||Tatsuhiro Okada||Fuel cell|
|US20030215686||4 Mar 2003||20 Nov 2003||Defilippis Michael S.||Method and apparatus for water management of a fuel cell system|
|US20040011648||17 Jul 2002||22 Jan 2004||Paul Phillip H.||Laminated flow device|
|US20040107996||9 Dec 2002||10 Jun 2004||Crocker Robert W.||Variable flow control apparatus|
|USRE35010||27 Sep 1993||8 Aug 1995||Nordson Corporation||Method of compensating for changes in the flow characteristics of a dispensed fluid to maintain the volume of dispensed fluid at a setpoint|
|CN2286429Y||4 Mar 1997||22 Jul 1998||中国科学技术大学||Porous core column electroosmosis pump|
|DE19625648A1||26 Jun 1996||30 Jan 1997||Hewlett Packard Co||Pumpsystem|
|EP0183950A1||17 Oct 1985||11 Jun 1986||Hewlett-Packard GmbH||Method of processing liquid within a tube|
|GB2303885A||Title not available|
|JP6197567A||Title not available|
|JP61237717A||Title not available|
|JPH0618964A||Title not available|
|JPH0772934A||Title not available|
|JPS6197567A||Title not available|
|JPS6468503A||Title not available|
|JPS50116893A||Title not available|
|JPS61237717A||Title not available|
|1||Adamson, A.W. et al., "Electrical Aspects of Surface Chemistry," Physical Chemistry of Surfaces, pp. 185-187 (Wiley, NY 1997).|
|2||Advisory Action for U.S. Appl. No. 09/942,884, mailed on May 24, 2005, 3 pages.|
|3||Agilent Technologies: Agilent 1100 Series Capillary LC System, A one-vendor solution for highest sensitivity and robustness, 7 pages.|
|4||Ananthakrishnan, V. et al., "Laminar Dispersion in Capillaries: Part I. Mathematical Analysis," A.I.Ch.E. Journal, 11(6):1063-1072 (Nov. 1965).|
|5||Aris, R, "On the dispersion of a solute in a fluid flowing through a tube," Oxidation of organic sulphides. VI, Proc. Rov. Soc. (London), 235A:67-77.|
|6||Burgreen, D. et al., "Electrokinetic Flow in Ultrafine Capillary Slits," The Journal of Physical Chemistry, 68:(5) 1084-1091.|
|7||Carvalho, R.T. et al. "Slow-flow measurements and fluid dynamics analysis using the Fresnel drag effect," Applied Optics, vol. 33, No. 25 (Sep. 1, 1994).|
|8||Chatwin, P.C. et al., "The effect of aspect ratio on longitudinal diffusivity in rectangular channels," J. Fluid Mech., 120:347-358 (1982).|
|9||Davis, M.T. et al., "Low Flow High-Performance Liquid Chromatography Solvent Delivery System Designed for Tandem Capillary Liquid Chromatography-Mass Spectrometry," Am. Soc. for Mass Spectrometry, 6:571-577 (1995).|
|10||Davis, M.T. et al., "Low Flow High-Performance Liquid Chromatography Solvent Delivery System Designed for Tandem Capillary Liquid Chromatography—Mass Spectrometry," Am. Soc. for Mass Spectrometry, 6:571-577 (1995).|
|11||Davis, M.T. et al., "Variable Flow Liquid Chromatography-Tandem Mass Spectrometry and the Comprehensive Analysis of Complex Protein Digest Mixtures," Am. Soc. For Mass Spectrometry, 8:1059-1069 (1997).|
|12||Desiderio at al., "A new electrode chamber for stable performance in capillary electrophoresis," Electrophoresis, 20:525-528 (1999).|
|13||Doshl, Mahendra R. et al., "Three Dimensional Laminar Dispersion in Open and Closed Rectangular Conduits," Chemical Engineering Science, 33:795-804 (1978).|
|14||Drott, J. et al., "Porous silicon as the carrier matrix in microstructured enzyme reactors yielding high enzyme activities," J. Micromech. Microeng. 7:14-23 (1997).|
|15||Enoksson, Peter et al., "A Silicon Resonant Sensor Structure for Coriolis Mass-Flow Measurements," Journal of Microelectromechanical Systems, vol. 6, No. 2 (Jun. 1997).|
|16||European Search Report, EP 02 73 9909.|
|17||Gan, W. et al. "Mechanism of porous core electroosmotic pump flow injection system and its application to determination of chromium(VI) in waste-water," Talanta 51:667-675 (2000).|
|18||International Preliminary Examination Report for PCT App. No. PCT/US02/19121, mailed Mar. 7, 2003, 4 pages.|
|19||International Search Report for PCT App. No. PCT/US02/19121, mailed Jan. 3, 2003, 4 pages.|
|20||International Search Report, for PCT Application No. PCT/US03/30008, mailed Mar. 4, 2004, 4 pages.|
|21||Jessensky O. et al., "Self-Organized Formation of Hexagonal Pore Structures in Anodic Alumina," J. Electrochem. Soc. 145(11) :3755-374 (Nov. 1998).|
|22||Johnson, David Linton et al., "Dependence of the conductivity of a porous medium on electrolyte conductivity," Physical Review Letters, 37(7):3502-3510 (Mar. 1, 1998).|
|23||Johnson, David Linton et al., "New Pore-Size Parameter Characterizing Transport in Porous Media," Physical Review Letters, 57(20):2564-2567 (Nov. 17, 1986)|
|24||Johnson, David Linton et al., "Theory dynamice permeability and tortouosity in fluid-saturated porous media," J. Fluid Mech. 176:379-402 (1987).|
|25||Kobatake, Y. et al., "Flows Through Charged Membranes. I. Flip-Flop Current vs Voltage Relation," J. Chem. Phys. 40(8):2212-2218 (Apr. 1964).|
|26||Kobatake, Y. et al., "Flows Through Charged Membranes. II. Oscillation Phenomena," J. Chem. Phys. 40(8):2219-2222 ( Apr. 1964).|
|27||Langridge, J. et al., "High Sensitivity Phosphoprotein Analysis Using a Combination of Variable Flow Chromatography and Precursor Ion Discovery on a Qtof Mass Spectrometer," Presented at 19th Montreux Symposium, Montreux, Switzerland, Nov. 6-8, 2002, 4 pages.|
|28||Langridge, J. et al., "Nano-Scale Variable Flow Chromatography For High Sensitivity Proteome Studies," Presented at COMBIO, Canberra, Australia, Sep. 30-Oct. 4, 2001, 4 pages.|
|29||LeBlanc, Jacques C., "The Stableflow Pump-a low-noise and drift-free pump for high performance liquid chromatography," Rev. Sci. Instrum. 62(6), 1642-1646 (Jun. 1991).|
|30||LeBlanc, Jacques C., "The Stableflow Pump—a low-noise and drift-free pump for high performance liquid chromatography," Rev. Sci. Instrum. 62(6), 1642-1646 (Jun. 1991).|
|31||Ma, Ying et al., "A review of zeolite-like porous materials," Microporous and Mesoporous Materials, 37:243-252 (2000).|
|32||Martin, S.E. et al., "Subfemtomole MS and MS/MS Peptide Sequence Analysis Using Nano-HPLC Micro-ESI Fourier Transform Ion Cyclotron Resonance Mass Spectrometry," Analytical Chemistry, 72(18):4266-4274 (2000).|
|33||McNair, H.M., "High Pressure Liquid Chromatography Equipment-II," Journal of Chromatographic Science (Aug. 1974).|
|34||McNair, H.M., "High Pressure Liquid Chromatography Equipment—II," Journal of Chromatographic Science (Aug. 1974).|
|35||Morrison, F.A. et al., "Electrokinetic Energy Conversion in Ultrafine Capillaries," J. Chem. Phys. 43:2111-2115 (1965).|
|36||Nakanishi, Kazuki et al., "Phase separation in silica sol-gel system containing polyacrylic acid, I. Gel formation behavior and effect of solvent composition," Journal of Crystalline Solids, 139:1-13 (1992).|
|37||Notice of Rejection for Japanese Patent Application No. 2003-504171, dated May 7, 2008, 3 pages (unofficial English translation).|
|38||Notice of Rejection for Japanese Patent Application No. 2004-538456, dated May 26, 2009, 3 pages.|
|39||Office Action for U.S. Appl. 10/246,284, mailed Jan. 27, 2006, 6 pages.|
|40||Office Action for U.S. Appl. 10/246,284, mailed Jul. 16, 2007, 6 pages.|
|41||Office Action for U.S. Appl. 10/246,284, mailed Sep. 30, 2005, 6 pages.|
|42||Office Action for U.S. Appl. No. 09/942,884 mailed on Apr. 22, 2004, 5 pages.|
|43||Office Action for U.S. Appl. No. 09/942,884 mailed on Aug. 12, 2004, 9 pages.|
|44||Office Action for U.S. Appl. No. 09/942,884 mailed on Mar. 9, 2005, 7 pages.|
|45||Office Action for U.S. Appl. No. 10/155,474 mailed on Jul. 13, 2005, 5 pages.|
|46||Office Action for U.S. Appl. No. 10/155,474 mailed on Nov. 4, 2005, 6 pages.|
|47||Office Action for U.S. Appl. No. 10/246,284, mailed Oct. 4, 2006, 5 pages.|
|48||Office Action for U.S. Appl. No. 10/480,619, mailed Oct. 9, 2007, 6 pages.|
|49||Office Action for U.S. Appl.. No. 10/155,474 mailed on Jul. 18, 2006, 6 pages.|
|50||Paul, P.H. et al., "Electrokinetic Generation Of High Pressures Using Porous Microstructures," Micro Total Analysis Systems, pp. 49-52 (1998).|
|51||Paul, P.H. et al., "Electrokinetic Pump Application in Micro-Total Analysis Systems Mechanical Actuation to HPLC," Micro Total Analysis Systems, pp. 583-590 (2000).|
|52||Peters, Eric C. et al., "Molded Rigid Polymer Monoliths as Separation Media for Capillary Electrochromatography," Anal. Chem., 69:3646-3649 (1997).|
|53||Philipse, Albert P., "Solid opaline packings of colloidal sillica spheres," Journal of Materials Science Letters, 8:1371-1373 (1989).|
|54||Rastogi, R.P., "Irreversible Thermodynamics of Electro-osmotic Effects," J. Scient. Ind. Res., (28):284-292 (Aug. 1969).|
|55||Rice, C. L. et al., "Electrokinetic Flow in a Narrow Cylindrical Capillary," The Journal of Physical Chemistry, 69(11):4017-4023 (Nov. 1966).|
|56||Rosen, Milton J., "2. Adsorption of Surface-Active Agents at Interfaces: The Electrical Double Sons Layer," Surfactants and Interfacial Phenomena, Second Ed., John Wiley & Sons.|
|57||Schmid, G. et al., "Electrochemistry of capillary systems with narrow pores V. Streaming potential: Donnan hindrance of electrolyte transport," J. Membrane Sci. 150:197-209 (1998).|
|58||Schmid, G., "Electrochemistry of capillary systems with narrow pores. II. Electroosmosis," J. Membrane Sci. 150:159-170 (1998).|
|59||Stahl, D.C., "Data-Controlled Automation of Liquid Chromatography/Tandem Mass Spectrometry Analysis of Peptide Mixtures," Am. Soc. For Mass Spectromtry, 7:532-540 (1996).|
|60||Taylor, Geoffrey, "Dispersion of soluble matter in solvent flowing slowly through a tube," Proc. Roy. Soc. (London) 21:186-203.|
|61||Vissers, J.P.C. et al., "A Novel Interface for Variable Flow Nanoscale LC/MS/MS for Improved Proteome Coverage," Am. Soc. for Mass Spectrometry, 13:760-771 (2002).|
|62||Vissers, Johannes P.C., "Recent developments in microcolumn liquid chromatography," Journal of Chromatography A, 856, 117-143 (1999).|
|63||Weston, Andrea et al., "Chapter 3 Instrumentation for High-Performance Liquid Chromatography," HPLC and CE, Principles and Practice, pp. 82-85, Academic Press.|
|64||Wijnhoven, Judith et al., "Preparation of Photonic Crystals Made of Air Spheres in Titania," Science, 281:802-804(Aug. 7, 1998).|
|65||Yazawa, T.,"Present Status and Future Potential of Preparation of Porous Glass and its Application," Key Engineering Materials, 115:125-146 (1995).|
|66||Zeng, S. et al., "Fabrication and characterization of electroosmotic micropumps," Sensors and Actuators, B 79:107-114 (2001).|
|Citing Patent||Filing date||Publication date||Applicant||Title|
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|US9416777||26 Sep 2014||16 Aug 2016||Becton, Dickinson And Company||Control circuits for electrochemical pump with E-valves|
|WO2014046806A1 *||13 Aug 2013||27 Mar 2014||Board Of Regents Of The University Of Texas System||Electro-osmotic pumps with electrodes comprising a lanthanide oxide or an actinide oxide|
|U.S. Classification||204/600, 204/450|
|International Classification||G05D11/13, G05D7/06, G01N30/32, B01D61/56|
|Cooperative Classification||Y10T137/85978, Y10T137/85986, Y10T137/86027, G01N2030/324, B01D61/56, G05D7/0694, G01N30/32, G01N2030/326, G05D11/132|
|European Classification||G05D7/06F4G, B01D61/56, G01N30/32, G05D11/13B2|
|18 Feb 2010||AS||Assignment|
Owner name: EKSIGENT TECHNOLOGIES LLC,CALIFORNIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:PAUL, PHILLIP H.;ARNOLD, DON WESLEY;BAILEY, CHRISTOPER G.;SIGNING DATES FROM 20011030 TO 20011031;REEL/FRAME:023958/0677
|12 Mar 2010||AS||Assignment|
Owner name: AB SCIEX LLC,MASSACHUSETTS
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:EKSIGENT TECHNOLOGIES, LLC;REEL/FRAME:024066/0427
Effective date: 20100212
Owner name: AB SCIEX LLC, MASSACHUSETTS
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:EKSIGENT TECHNOLOGIES, LLC;REEL/FRAME:024066/0427
Effective date: 20100212
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