US3618734A - Specimen incubator - Google Patents

Specimen incubator Download PDF

Info

Publication number
US3618734A
US3618734A US831957A US3618734DA US3618734A US 3618734 A US3618734 A US 3618734A US 831957 A US831957 A US 831957A US 3618734D A US3618734D A US 3618734DA US 3618734 A US3618734 A US 3618734A
Authority
US
United States
Prior art keywords
incubator
cabinet
specimen
trays
opening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US831957A
Inventor
Waheed N Khan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Res Foundation Of Children S H
RESEARCH FOUNDATION OF CHILDREN'S HOSPITAL OF DISTRICT OF COLUMBIA
Original Assignee
Res Foundation Of Children S H
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Foundation Of Children S H filed Critical Res Foundation Of Children S H
Application granted granted Critical
Publication of US3618734A publication Critical patent/US3618734A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346Heating or cooling arrangements
    • G01N2035/00356Holding samples at elevated temperature (incubation)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/0401Sample carriers, cuvettes or reaction vessels
    • G01N2035/0406Individual bottles or tubes
    • G01N2035/0408Individual bottles or tubes connected in a flexible chain
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/809Incubators or racks or holders for culture plates or containers

Definitions

  • a specimen incubator including a closed cabinet having an access opening which can be selectively opened and closed, and wherein are mounted a plurality of specimen support trays movable in a path in the cabinet in a manner for selective placement of any given tray at the access opening, and further including means for controllably circulating a gaseous medium in the cabinet with means provided to preclude entrance of external atmosphere into the incubator under open conditions thereof, the incubator preferably including pluralities of specimen support trays in vertically separate tiers, and movement of the trays in the individual tiers being individually separately controllable.
  • the present invention permits easy insertion and removal of a plurality of individual specimens in the incubator, and incorporates means operable in such a manner as to render each individual specimen readily and easily accessible to a technician.
  • the invention also includes a structure whereby external atmosphere, which might invite contamination within an incubator, is precluded when an access opening into the incubator is used for insertion or removal of a test specimen.
  • FIG. 1 is a perspective view of an incubator according to the invention, arranged on a support, and showing an access door in closed position;
  • FIG. 2 is a vertical elevational view of the incubator with a frzmt wall removed to disclose a specimen tray arrangement and schematically air circulation flow;
  • FIG. 3 is a horizontal sectional view of the invention generally on line 3-3 of FIG. 2, parts being broken away, and disclosing in greater clarity the arrangement of air circulating means including a spacing shelf and circulation apertures therein;
  • FIG. 4 is a fragmentary vertical sectional view illustrating structure adapted to maintain desired atmospheric conditions within the incubator with an open access door;
  • FIG. 5 is a fragmentary enlarged sectional view generally along line 55 of FIG. 4, with the access door closed;
  • FIG. 6 is a view similar to FIG. 5 with the access door open;
  • FIG. 7 is an enlarged fragmentary view disclosing a portion of drive mechanism for specimen trays or containers, shown operatively connected thereto;
  • FIG. 8 is a vertical sectional view of a specimen tray and specimen tube-supporting means thereon;
  • FIG. 9 is a top plan view of the tray of FIG. 8;
  • FIG. 10 is a view similar to FIG. 9 showing a modified specimen tube support.
  • FIG. 11 is a fragmentary schematic plan view showing drive mechanism for specimen trays, taken generally along lines 11-11 ofFIG. 7.
  • the incubator of the present invention includes a cabinet 22 adapted for placement on any suitable support such as shown for example in broken lines at 24.
  • Inspection and viewing windows 26 are provided in the front and rear of the cabinet to permit readily viewing the interior of the cabinet and these windows can, if desired, be provided with tinted glass for light control.
  • Access doors 28 are provided in the front and rear sides of the cabinet, and are selectively closable by means of sliding glass, or other transparent material, doors or panels 30, and having operating handles 32. Any suitable support and guide track means can be provided for the sliding doors 30, and preferably maintain a relatively tight sealing engagement with these doors to insure controlled atmospheric conditions within the incubator.
  • a plurality of vertically spaced shelves 34 are mounted in the cabinet, and can, if desired, be removable.
  • the shelves have a plurality of circulation openings 36 therethrough, to permit airflow and circulation in the cabinet and the inner compartmented structure thereof.
  • a plurality of specimen trays 38 are arranged in vertically spaced tiers as shown in FIG. 2, above the respective shelves 34, and are adapted to be moved, selectively in the individual tiers, in paths 40 whereby the individual trays can be positioned at one of the access doors 28 for specimen placement in the trays or removal therefrom by an operator or technician.
  • the individual trays can consist of generally cylindrical shaped containers 42 having closed bottoms 44 and open tops 46. The sides of the cylinders are open as indicated at 48 for access to the tray, and additionally for viewing of specimens placed therein.
  • Shafts 50 are afiixed to the bottoms of the trays by any desired means such as by hubs 52, the shafts depending vertically downwardly from the trays.
  • FIG. 11 of the drawings Movement of the trays along the paths, in each of the tiers, and support of the individual trays, will be readily apparent from FIG. 11 of the drawings.
  • Upper and lower link chains 54 and 56 are supported on hubbed sprockets 58 and 60 supported on the shelves in spaced positions along the paths, and in the embodiment shown at the four corners of the paths.
  • the sprocket wheels engage in the chain link openings in a usual manner for operatively driving the chains.
  • Chain tensioners 62 are mounted at the corners of the paths to properly maintain driving tension on the chains, and to give adequate support for the containers.
  • the specimen trays or containers have their shafts 50 inserted in spaced ones of open chain link connecting pins 64 of the chains.
  • the provision of the upper and lower chain runs serve to support the specimen trays, through the shafts 50, in a vertical position, and, additionally, as the chains are moved in the respective paths will convey the individual specimen trays around the paths in an obvious manner.
  • the drive means whereby the chains and trays can be moved includes motor 66 and gear reduction box 68, supported on brackets 70 or the like.
  • Output shaft 72 is connected to one-half of a separable pin-type coupling, generally designated 74, with the lowermost portion thereof being operatively spring tensioned by spring 76 into coupling relationship with the upper portion thereof.
  • a handle 78 connected to the lowermost coupling part is adapted for manual movement thereof, as indicated by the arrow in FIG. 7, for manual coupling or uncoupling.
  • a handwheel 81 is connected to shaft 80 of the sprockets, as is the uppermost portion of the coupling, and upon disengagement of the coupling with the handwheel can be used for manual movement of the specimen trays along the paths.
  • drive means for the different sets of chains are individually provided and, as will be pointed out hereinafter, are selectively and individually operable for separate movement of the different sets of trays. Obviously, only one drive mechanism is needed for each of the sets of chains.
  • a fan blower 82 is mounted in the cabinet, including a motor and fan as shown in FIG. 2, and within, as regards the fan, a housing 84 within which is also mounted filter 86 and resistance heater 88.
  • housing 84 The inlet end of housing 84 is open to permit air within the incubator to enter and be driven by the fan through the filter and over the heater toward a clos ing flange 90 or the like having, in the embodiment shown, two openings 92 in proximity to the periphery thereof, to which are connected conduits 94 which extend to proximity with access doors 28, and terminate in perforated end conduit sections 96.
  • the flange 90 is also provided with a centrally disposed air opening 96.
  • the arrangement of the conduits and opening is such that, dependent upon operation of other components of the incubator, a flow of air is established as indicated by the arrows through the confines of the incubator when the access doors are closed, and when the access doors are open will create an air film or curtain across the access openings to prevent entry of outside air into the incubator.
  • Means, as will appear hereinafter, are provided to restrict airflow through the perforated conduit sections 96 when the doors are closed. Under normal operation of the incubator, it is desirable to maintain a temperature therein in the neighborhood of 37 C. although, as will be readily understood, this can be varied as dictated by operating requirements for the incubator. Control of the air heater effects this operation.
  • Cages 98 are attached to the cabinet around the access openings and within which the access doors 28 are slidably contained and in sealing relationship with respect to the interior and exterior of the cabinet.
  • An air inlet opening 100 is provided in each of the conduits 94 at its juncture point with the perforated end sections 94A.
  • the doors have valve plates 102 formed as extensions thereof, or as plates affixed thereto, and which are operable upon closing of an access door to obturate air inlet openings 100 to preclude airflow from the conduit into and through the perforated sections.
  • the doors when closed additionally prevent entrance of exterior air into the incubator. Airflow to form the film or curtain upon opening a door is indicated by arrows 104 in FIGS. 1 and 2.
  • Circulation within the incubator is possible by means of, including, the apertures 36, the flow being indicated by arrows 106. If desired, air leakage can be provided for in connection with the air inlet opening 100 and plate valve 102 to further ensure against entry ofexterior air into the incubator.
  • Control of operation of the incubator is accomplished by a plurality of switches mounted on the exterior of the cabinet generally as indicated at 108.
  • Motor switch sets M8,, M8 and MS are operatively connected to respective ones of motors 66 to operate the chains in the various tiers.
  • the motors being reversible, the left-hand ones of the switch buttons will operate the chains in one direction, and conversely the righthand switches will operate the chains in the other direction.
  • the blower motor is operated by switch B and resistance heater is actuated by switch T.
  • This is a variable temperature switch or rheostat, the control factor being indicated generally at 110 in FIG. 2,
  • the shelves 34 can be provided with upraised ridges 112, FIG. 3, and boxes 114 adapted to contain specimen material or the like can be supported on the shelves, and restrained thereon by means ofthe ridge.
  • FIGS. 8-10 inclusive means can be incorporated for supporting test tubes or the like.
  • this includes an upper open ring 116 and a closed bottom cup 118.
  • the upper ring is replaced by a spring clip 120 of a known construction, and
  • the incubator of the invention permits quick and easy disposition of any specimen within the incubator for easy access at the front and rear sides by operation of the various motor control switches.
  • the condition of the air within the incubator is also readily controllable by means of the door structures and the control means for the air flow within the incubator in the manner hereinbefore described.
  • the present incubator has desirable small dimensions adapted to contain a plurality of specimens, each of which is readily available through easy access means, and the size of the incubator permits plural placement in desirable use areas.
  • the multiplicity of specimens which can be placed in such an incubator in a centrally located position saves a substantial amount of time in a technician s placement or removal to increase available incubation time. This saving in time results in speeding up operations where quick results are desired, such as in sensitivity tests and the like, due to minimizing time required following taking a specimen and placement in an incubator, and subsequent observation, and saving in overall reporting time.
  • the number of technicians required under normal operating conditions is very substantially reduced due to the convenience and operation of, and selective placement of a plurality of these incubators.
  • a specimen incubator comprising:
  • E. housed controllable gas circulating means comprising a selectively operable fan and gas flow conduit leading from said housed means and terminating in a perforated end-portion positioned exteriorly on the said cabinet adjacent to said access opening;
  • said gas-circulating means operable when the closure means are opened to thereby prevent entrance of external atmosphere into the said cabinet.
  • a specimen incubator as claimed in claim 1, wherein the closure means for said access opening comprises a slidable door in sealing engagement with said access opening when in closed position, said conduit having an air opening therein preceding said perforated end portion, said door having a plate valve thereon adapted for insertion in said air opening in said conduit with said door in closed position to preclude flow through said perforations, and said housing having an opening therein for directing gas medium flow therefrom into the interior of the cabinet and through the shelf openings for circulation through and about the cabinet interior in a recirculating cycle of the medium.
  • a specimen incubator as claimed in claim 2 and further including a filter and an adjustable heater in said housing for filtration and temperature control of the gaseous medium circulated within said cabinet.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

A specimen incubator including a closed cabinet having an access opening which can be selectively opened and closed, and wherein are mounted a plurality of specimen support trays movable in a path in the cabinet in a manner for selective placement of any given tray at the access opening, and further including means for controllably circulating a gaseous medium in the cabinet with means provided to preclude entrance of external atmosphere into the incubator under open conditions thereof, the incubator preferably including pluralities of specimen support trays in vertically separate tiers, and movement of the trays in the individual tiers being individually separately controllable.

Description

United States Patent [72] inventor Waheed N. Khan Silver Spring, Md.
[2i] Appl. No. 831,957
[22] Filed June 10, 1969 [45] Patented Nov. 9, 1971 [73] Assignee Research Foundation of Children s Hospital of the District at Columbia Washington, [LC
[54] SPECIMEN INCUBATOR 127.142; 119/15. 35 153/15, 51, s4;2|/s0; 34/205, 21 l, 2 i6, 233; 99/182 [56] Reterences Cited UNITED STATES PATENTS 2,325,368 7Il943 Christensen l95/i42 2,703,570 3/l955 Young i l9/i5 3,002,895 l0/l96l Freedman I 95/ i 43 3,302,6l5 2/i967 Tietje I l9/l5 Primary Examiner-Alvin E. Taneuhoitz Anomey.l. Gibson Semmes ABSTRACT: A specimen incubator including a closed cabinet having an access opening which can be selectively opened and closed, and wherein are mounted a plurality of specimen support trays movable in a path in the cabinet in a manner for selective placement of any given tray at the access opening, and further including means for controllably circulating a gaseous medium in the cabinet with means provided to preclude entrance of external atmosphere into the incubator under open conditions thereof, the incubator preferably including pluralities of specimen support trays in vertically separate tiers, and movement of the trays in the individual tiers being individually separately controllable.
SHEET 1 [1F 2 PATENTEDNOV 91971 SPECIMEN INCUBATOR BACKGROUND OF THE INVENTION Heretofore, specimen incubator systems and arrangements in facilities such as hospitals, laboratories and the like, such as used in microbiological studies, for example, have been cumbersome and inconvenient in structure and placement within a facility. In many such known installations a centrally located communally used area was provided which, in many instances, required a substantial amount of a technician's time in transporting specimens to the area, and in such areas the arrangement was such that placement and arrangement of the specimens, observation thereof, removal for study and reporting required considerable periods of time, and in many instances necessitated inconvenient operations of a tedious nature. In such hitherto known arrangements there was, accordingly, a very substantial time loss factor for the laboratory technicians, as well as a highly undesirable delay in obtaining results of specimen culturing, studies, and reporting, with possible serious results stemming therefrom.
SUMMARY OF THE INVENTION It is a principle feature of the present invention to provide a specimen incubator of the nature in question, which overcomes drawbacks and undesirable features in previous known systems or installations, and which provides a self-contained incubator having such dimensions, construction and operation as to be easily placed in one or more convenient locations for use, and which is capable of holding a plurality of test specimens in such a manner that they are easily accessible for placement, observation, and removal at areas readily and quickly available for a technician. Time delays in overall incubation time and obtaining results thereof is substantially minimized, and results can be made available much more rapidly with a very substantial saving in technicians time. The present invention permits easy insertion and removal of a plurality of individual specimens in the incubator, and incorporates means operable in such a manner as to render each individual specimen readily and easily accessible to a technician. The invention also includes a structure whereby external atmosphere, which might invite contamination within an incubator, is precluded when an access opening into the incubator is used for insertion or removal of a test specimen.
Other and additional objects of the invention will be more readily understood from the following detailed description of an embodiment thereof when taken together with the accompanying drawings, in which:
FIG. 1 is a perspective view of an incubator according to the invention, arranged on a support, and showing an access door in closed position;
FIG. 2 is a vertical elevational view of the incubator with a frzmt wall removed to disclose a specimen tray arrangement and schematically air circulation flow;
FIG. 3 is a horizontal sectional view of the invention generally on line 3-3 of FIG. 2, parts being broken away, and disclosing in greater clarity the arrangement of air circulating means including a spacing shelf and circulation apertures therein;
FIG. 4 is a fragmentary vertical sectional view illustrating structure adapted to maintain desired atmospheric conditions within the incubator with an open access door;
FIG. 5 is a fragmentary enlarged sectional view generally along line 55 of FIG. 4, with the access door closed;
FIG. 6 is a view similar to FIG. 5 with the access door open;
FIG. 7 is an enlarged fragmentary view disclosing a portion of drive mechanism for specimen trays or containers, shown operatively connected thereto;
FIG. 8 is a vertical sectional view of a specimen tray and specimen tube-supporting means thereon;
FIG. 9 is a top plan view of the tray of FIG. 8;
FIG. 10 is a view similar to FIG. 9 showing a modified specimen tube support; and
FIG. 11 is a fragmentary schematic plan view showing drive mechanism for specimen trays, taken generally along lines 11-11 ofFIG. 7.
Referring now in detail to the drawings, the incubator of the present invention, generally designated 20, includes a cabinet 22 adapted for placement on any suitable support such as shown for example in broken lines at 24. Inspection and viewing windows 26 are provided in the front and rear of the cabinet to permit readily viewing the interior of the cabinet and these windows can, if desired, be provided with tinted glass for light control. Access doors 28 are provided in the front and rear sides of the cabinet, and are selectively closable by means of sliding glass, or other transparent material, doors or panels 30, and having operating handles 32. Any suitable support and guide track means can be provided for the sliding doors 30, and preferably maintain a relatively tight sealing engagement with these doors to insure controlled atmospheric conditions within the incubator.
A plurality of vertically spaced shelves 34 are mounted in the cabinet, and can, if desired, be removable. The shelves have a plurality of circulation openings 36 therethrough, to permit airflow and circulation in the cabinet and the inner compartmented structure thereof.
A plurality of specimen trays 38 are arranged in vertically spaced tiers as shown in FIG. 2, above the respective shelves 34, and are adapted to be moved, selectively in the individual tiers, in paths 40 whereby the individual trays can be positioned at one of the access doors 28 for specimen placement in the trays or removal therefrom by an operator or technician. The individual trays can consist of generally cylindrical shaped containers 42 having closed bottoms 44 and open tops 46. The sides of the cylinders are open as indicated at 48 for access to the tray, and additionally for viewing of specimens placed therein. Shafts 50 are afiixed to the bottoms of the trays by any desired means such as by hubs 52, the shafts depending vertically downwardly from the trays. Movement of the trays along the paths, in each of the tiers, and support of the individual trays, will be readily apparent from FIG. 11 of the drawings. Upper and lower link chains 54 and 56 are supported on hubbed sprockets 58 and 60 supported on the shelves in spaced positions along the paths, and in the embodiment shown at the four corners of the paths. The sprocket wheels engage in the chain link openings in a usual manner for operatively driving the chains. Chain tensioners 62 are mounted at the corners of the paths to properly maintain driving tension on the chains, and to give adequate support for the containers. The specimen trays or containers have their shafts 50 inserted in spaced ones of open chain link connecting pins 64 of the chains. The provision of the upper and lower chain runs serve to support the specimen trays, through the shafts 50, in a vertical position, and, additionally, as the chains are moved in the respective paths will convey the individual specimen trays around the paths in an obvious manner.
The drive means whereby the chains and trays can be moved includes motor 66 and gear reduction box 68, supported on brackets 70 or the like. Output shaft 72 is connected to one-half of a separable pin-type coupling, generally designated 74, with the lowermost portion thereof being operatively spring tensioned by spring 76 into coupling relationship with the upper portion thereof. A handle 78 connected to the lowermost coupling part is adapted for manual movement thereof, as indicated by the arrow in FIG. 7, for manual coupling or uncoupling. A handwheel 81 is connected to shaft 80 of the sprockets, as is the uppermost portion of the coupling, and upon disengagement of the coupling with the handwheel can be used for manual movement of the specimen trays along the paths. As will be seen, drive means for the different sets of chains are individually provided and, as will be pointed out hereinafter, are selectively and individually operable for separate movement of the different sets of trays. Obviously, only one drive mechanism is needed for each of the sets of chains.
In addition to the foregoing described important feature, whereby any individual specimen tray can be brought to a position in front of one of the access doors, it is also of substantial importance that means be provided for ensuring that the ambient atmosphere within the incubator be maintained uniform throughout at the proper temperature, with means also being incorporated for filtering of air within the incubator. To this end, a fan blower 82 is mounted in the cabinet, including a motor and fan as shown in FIG. 2, and within, as regards the fan, a housing 84 within which is also mounted filter 86 and resistance heater 88. The inlet end of housing 84 is open to permit air within the incubator to enter and be driven by the fan through the filter and over the heater toward a clos ing flange 90 or the like having, in the embodiment shown, two openings 92 in proximity to the periphery thereof, to which are connected conduits 94 which extend to proximity with access doors 28, and terminate in perforated end conduit sections 96. The flange 90 is also provided with a centrally disposed air opening 96. The arrangement of the conduits and opening is such that, dependent upon operation of other components of the incubator, a flow of air is established as indicated by the arrows through the confines of the incubator when the access doors are closed, and when the access doors are open will create an air film or curtain across the access openings to prevent entry of outside air into the incubator. Means, as will appear hereinafter, are provided to restrict airflow through the perforated conduit sections 96 when the doors are closed. Under normal operation of the incubator, it is desirable to maintain a temperature therein in the neighborhood of 37 C. although, as will be readily understood, this can be varied as dictated by operating requirements for the incubator. Control of the air heater effects this operation.
Cages 98 are attached to the cabinet around the access openings and within which the access doors 28 are slidably contained and in sealing relationship with respect to the interior and exterior of the cabinet. An air inlet opening 100 is provided in each of the conduits 94 at its juncture point with the perforated end sections 94A. The doors have valve plates 102 formed as extensions thereof, or as plates affixed thereto, and which are operable upon closing of an access door to obturate air inlet openings 100 to preclude airflow from the conduit into and through the perforated sections. The doors when closed additionally prevent entrance of exterior air into the incubator. Airflow to form the film or curtain upon opening a door is indicated by arrows 104 in FIGS. 1 and 2. Circulation within the incubator is possible by means of, including, the apertures 36, the flow being indicated by arrows 106. If desired, air leakage can be provided for in connection with the air inlet opening 100 and plate valve 102 to further ensure against entry ofexterior air into the incubator.
Control of operation of the incubator is accomplished by a plurality of switches mounted on the exterior of the cabinet generally as indicated at 108. Motor switch sets M8,, M8 and MS, are operatively connected to respective ones of motors 66 to operate the chains in the various tiers. The motors being reversible, the left-hand ones of the switch buttons will operate the chains in one direction, and conversely the righthand switches will operate the chains in the other direction. The blower motor is operated by switch B and resistance heater is actuated by switch T. This is a variable temperature switch or rheostat, the control factor being indicated generally at 110 in FIG. 2,
If desired, the shelves 34 can be provided with upraised ridges 112, FIG. 3, and boxes 114 adapted to contain specimen material or the like can be supported on the shelves, and restrained thereon by means ofthe ridge.
Referring to FIGS. 8-10 inclusive, means can be incorporated for supporting test tubes or the like. In FIGS. 8 and 9 this includes an upper open ring 116 and a closed bottom cup 118. In the modification shown in FIG. 10, the upper ring is replaced by a spring clip 120 of a known construction, and
serves to increase stability of a supported test tube From the foregoing description, it will be readily seen that the incubator of the invention permits quick and easy disposition of any specimen within the incubator for easy access at the front and rear sides by operation of the various motor control switches. The condition of the air within the incubator is also readily controllable by means of the door structures and the control means for the air flow within the incubator in the manner hereinbefore described.
Under certain conditions, it is desirable to have a controllable carbon dioxide content within the incubator. To this end, a supply of carbon dioxide, not shown, can be introduced into the incubator by manipulation of C0, button C.
It will be apparent that the present incubator has desirable small dimensions adapted to contain a plurality of specimens, each of which is readily available through easy access means, and the size of the incubator permits plural placement in desirable use areas. The multiplicity of specimens which can be placed in such an incubator in a centrally located position saves a substantial amount of time in a technician s placement or removal to increase available incubation time. This saving in time results in speeding up operations where quick results are desired, such as in sensitivity tests and the like, due to minimizing time required following taking a specimen and placement in an incubator, and subsequent observation, and saving in overall reporting time. The number of technicians required under normal operating conditions is very substantially reduced due to the convenience and operation of, and selective placement of a plurality of these incubators.
Manifestly, minor changes can be effected in the invention without departing from the spirit and scope thereof, as defined in, and limited solely by the appended claims.
1. A specimen incubator comprising:
A. a closed cabinet having an access opening;
B. means to selectively open and close said opening;
C. a plurality of vertically spaced shelves having openings therein within said cabinet;
D. a plurality of individually tiered arrays of specimen support trays above said shelves, means for mounting said trays for movement along a path within said cabinet and means operable for selective placement of any of said trays at said opening;
E. housed controllable gas circulating means, comprising a selectively operable fan and gas flow conduit leading from said housed means and terminating in a perforated end-portion positioned exteriorly on the said cabinet adjacent to said access opening; and
F. said gas-circulating means operable when the closure means are opened to thereby prevent entrance of external atmosphere into the said cabinet.
2. A specimen incubator as claimed in claim 1, wherein the closure means for said access opening comprises a slidable door in sealing engagement with said access opening when in closed position, said conduit having an air opening therein preceding said perforated end portion, said door having a plate valve thereon adapted for insertion in said air opening in said conduit with said door in closed position to preclude flow through said perforations, and said housing having an opening therein for directing gas medium flow therefrom into the interior of the cabinet and through the shelf openings for circulation through and about the cabinet interior in a recirculating cycle of the medium.
3. A specimen incubator as claimed in claim 2, and further including a filter and an adjustable heater in said housing for filtration and temperature control of the gaseous medium circulated within said cabinet.

Claims (2)

  1. 2. A specimen incubator as claimed in claim 1, wherein the closure means for said access opening comprises a slidable door in sealing engagement with said access opening when in closed position, said conduit having an air opening therein preceding said perforated end portion, said door having a plate valve thereon adapted for insertion in said air opening in said conduit with said door in closed position to preclude flow through said perforations, and said housing having an opening therein for directing gas medium flow therefrom into the interior of the cabinet and through the shelf openings for circulation through and about the cabinet interior in a recirculating cycle of the medium.
  2. 3. A specimen incubator as claimed in claim 2, and further including a filter and an adjustable heater in said housing for filtration and temperature control of the gaseous medium circulated within said cabinet.
US831957A 1969-06-10 1969-06-10 Specimen incubator Expired - Lifetime US3618734A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83195769A 1969-06-10 1969-06-10

Publications (1)

Publication Number Publication Date
US3618734A true US3618734A (en) 1971-11-09

Family

ID=25260292

Family Applications (1)

Application Number Title Priority Date Filing Date
US831957A Expired - Lifetime US3618734A (en) 1969-06-10 1969-06-10 Specimen incubator

Country Status (1)

Country Link
US (1) US3618734A (en)

Cited By (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4005679A (en) * 1974-02-13 1977-02-01 Pas Henricus G H M Method and apparatus for pre-incubating eggs
US4219529A (en) * 1977-11-28 1980-08-26 Eastman Kodak Company Incubator for chemical analyzer
US4250266A (en) * 1979-12-19 1981-02-10 Honeywell Inc. Automated micro-organism culture growth and detection instrument
US4301252A (en) * 1980-04-04 1981-11-17 Baker Fraser L Controlled environment incubator for light microscopy
EP0203869A1 (en) * 1985-05-30 1986-12-03 MASSON, Alain Device based on crystalline mineral material related to an energy source and the use of this device to improve bacterial metabolism
US4966853A (en) * 1987-07-20 1990-10-30 Kirin Beer Kabushiki Kaisha Cell culturing apparatus
US5061448A (en) * 1988-04-29 1991-10-29 Barnstead Thermolyne Corporation Incubator
US5346050A (en) * 1993-01-28 1994-09-13 Fleetwood Systems, Inc. Carton conveying apparatus
US5453077A (en) * 1993-12-17 1995-09-26 Hill-Rom Company, Inc. Infant thermal support device
EP0759464A2 (en) * 1995-08-22 1997-02-26 Venturedyne Ltd. Cytogenetic chamber and related method
US5681492A (en) * 1995-02-17 1997-10-28 Van Praet; Peter Incubator for micro titer plates
US5759149A (en) * 1993-12-17 1998-06-02 Hill-Rom, Inc. Patient thermal support device
US5789241A (en) * 1995-10-04 1998-08-04 Samsung Electronics Co., Ltd. Garbage decomposing apparatus having blower forming an air curtain blocking escape of odors
US5817003A (en) * 1993-12-17 1998-10-06 Hill-Rom, Inc. Controller for a patient warming device
US5976871A (en) * 1997-07-02 1999-11-02 Venturedyne, Ltd. Cytogenetic chamber
US6178921B1 (en) * 1998-04-16 2001-01-30 L'air Liquide Method of improving breeding conditions in newborn pigs
US20020047311A1 (en) * 1996-02-09 2002-04-25 Thermo Forma Inc. Controlled atmosphere incubator
US6469285B2 (en) * 2000-06-13 2002-10-22 Shimadzu Corporation Automatic temperature control device
US6611978B1 (en) 1999-11-15 2003-09-02 Hill-Rom Services, Inc. Patient-support apparatus
US6709384B1 (en) 1993-12-17 2004-03-23 Hill-Rom Services, Inc. Infant thermal support device
US20040147012A1 (en) * 2002-11-19 2004-07-29 Yasuhiko Yokoi Storage apparatus
US6880188B1 (en) 1999-11-15 2005-04-19 Draeger Medical Infant Care, Inc. Infant care apparatus with movable infant support
US20050115773A1 (en) * 2003-10-27 2005-06-02 Samsung Electronics Co., Ltd. Lift for substrate
US20080247628A1 (en) * 2005-10-14 2008-10-09 Unisense Fertilitech A/S Determination of a Change in a Cell Population
WO2010003423A2 (en) * 2008-07-05 2010-01-14 Unisense Fertilitech A/S One to one identification system
US20100041090A1 (en) * 2006-06-16 2010-02-18 Unisense Fertilitech A/S Embryo quality assessment based on blastomere division and movement
US20110165609A1 (en) * 2007-06-29 2011-07-07 Ramsing Niels B Device, a system and a method for monitoring and/or cultivation of microscopic objects
US20110183367A1 (en) * 2002-12-23 2011-07-28 Lars Ditlev Morck Ottosen Device and method for non-invasive measurement of the individual metabolic rate of a substantially spherical metabolizing particle
US20150122190A1 (en) * 2013-11-04 2015-05-07 Rose Acre Farms, Inc. Aviary walkway and ventilation system and method of circulating air in an aviary
US20160018429A1 (en) * 2010-09-08 2016-01-21 Tecan Trading Ag Microplate-reader with a controlled gas atmosphere and a corresponding method of controlling the gas atmosphere
WO2016149527A1 (en) * 2015-03-19 2016-09-22 President And Fellows Of Harvard College Incubating enclosure
US10779513B2 (en) 2013-11-04 2020-09-22 Rose Acre Farms, Inc. Aviary building construction system and method
US11041655B2 (en) 2013-11-04 2021-06-22 Rose Acre Farms, Inc. Aviary ventilation system and method
US11044892B2 (en) 2013-11-04 2021-06-29 Rose Acre Farms, Inc. Aviary system and method of circulating litter in an aviary
US11185053B2 (en) 2016-07-06 2021-11-30 Rose Acre Farms, Inc. Walkway and walkway system for an aviary

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2325368A (en) * 1940-10-07 1943-07-27 Nat Agrol Company Inc Apparatus for promoting mold growth
US2703570A (en) * 1952-06-12 1955-03-08 Univ Minnesota Apparatus for production of quadruped mammals
US3002895A (en) * 1957-02-11 1961-10-03 Freedman David Incubator-shaker apparatus
US3302615A (en) * 1965-04-15 1967-02-07 Becton Dickinson Co Controlled environmental system for handling and rearing laboratory and research animals

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2325368A (en) * 1940-10-07 1943-07-27 Nat Agrol Company Inc Apparatus for promoting mold growth
US2703570A (en) * 1952-06-12 1955-03-08 Univ Minnesota Apparatus for production of quadruped mammals
US3002895A (en) * 1957-02-11 1961-10-03 Freedman David Incubator-shaker apparatus
US3302615A (en) * 1965-04-15 1967-02-07 Becton Dickinson Co Controlled environmental system for handling and rearing laboratory and research animals

Cited By (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4005679A (en) * 1974-02-13 1977-02-01 Pas Henricus G H M Method and apparatus for pre-incubating eggs
US4219529A (en) * 1977-11-28 1980-08-26 Eastman Kodak Company Incubator for chemical analyzer
US4250266A (en) * 1979-12-19 1981-02-10 Honeywell Inc. Automated micro-organism culture growth and detection instrument
US4301252A (en) * 1980-04-04 1981-11-17 Baker Fraser L Controlled environment incubator for light microscopy
EP0203869A1 (en) * 1985-05-30 1986-12-03 MASSON, Alain Device based on crystalline mineral material related to an energy source and the use of this device to improve bacterial metabolism
FR2582669A1 (en) * 1985-05-30 1986-12-05 Masson Alain DEVICE BASED ON MINERAL CRYSTALLINE MATERIAL CONNECTED TO AN ENERGY SOURCE AND USE OF SAID DEVICE FOR IMPROVING BACTERIAL METABOLISM
US4762795A (en) * 1985-05-30 1988-08-09 Alain Masson Device based on an inorganic crystalline matter connected to a source of energy and use of said device for improving bacterial metabolism
US4966853A (en) * 1987-07-20 1990-10-30 Kirin Beer Kabushiki Kaisha Cell culturing apparatus
US5061448A (en) * 1988-04-29 1991-10-29 Barnstead Thermolyne Corporation Incubator
US5346050A (en) * 1993-01-28 1994-09-13 Fleetwood Systems, Inc. Carton conveying apparatus
US5971914A (en) * 1993-12-17 1999-10-26 Hill-Rom Company, Inc. Infant thermal support device
US7008371B2 (en) 1993-12-17 2006-03-07 Draeger Medical Infant Care, Inc. Patient thermal support device
US6709384B1 (en) 1993-12-17 2004-03-23 Hill-Rom Services, Inc. Infant thermal support device
US5759149A (en) * 1993-12-17 1998-06-02 Hill-Rom, Inc. Patient thermal support device
US6746394B2 (en) 1993-12-17 2004-06-08 Hill-Rom Services, Inc. Infant thermal support device
US5817002A (en) * 1993-12-17 1998-10-06 Hill-Rom Company, Inc. Infant thermal support device
US5817003A (en) * 1993-12-17 1998-10-06 Hill-Rom, Inc. Controller for a patient warming device
US6296606B1 (en) 1993-12-17 2001-10-02 Charles Goldberg Patient thermal support device
US5453077A (en) * 1993-12-17 1995-09-26 Hill-Rom Company, Inc. Infant thermal support device
US6270452B1 (en) 1993-12-17 2001-08-07 Hill-Rom Services, Inc. Infant thermal support device
US6761682B2 (en) 1993-12-17 2004-07-13 Hill-Rom Services, Inc. Patient thermal support device
US6036634A (en) * 1993-12-17 2000-03-14 Hill-Rom Company, Inc. Patient thermal support device
US5681492A (en) * 1995-02-17 1997-10-28 Van Praet; Peter Incubator for micro titer plates
EP0759464A3 (en) * 1995-08-22 2000-02-02 Venturedyne Ltd. Cytogenetic chamber and related method
EP0759464A2 (en) * 1995-08-22 1997-02-26 Venturedyne Ltd. Cytogenetic chamber and related method
US5851790A (en) * 1995-08-22 1998-12-22 Venturedyne, Ltd. Method for drying cells using cytogenetic chamber
US5789241A (en) * 1995-10-04 1998-08-04 Samsung Electronics Co., Ltd. Garbage decomposing apparatus having blower forming an air curtain blocking escape of odors
US6503751B2 (en) * 1996-02-09 2003-01-07 Thermo Forma Inc. Controlled atmosphere incubator
US20020047311A1 (en) * 1996-02-09 2002-04-25 Thermo Forma Inc. Controlled atmosphere incubator
US5976871A (en) * 1997-07-02 1999-11-02 Venturedyne, Ltd. Cytogenetic chamber
US6178921B1 (en) * 1998-04-16 2001-01-30 L'air Liquide Method of improving breeding conditions in newborn pigs
US6880188B1 (en) 1999-11-15 2005-04-19 Draeger Medical Infant Care, Inc. Infant care apparatus with movable infant support
US6611978B1 (en) 1999-11-15 2003-09-02 Hill-Rom Services, Inc. Patient-support apparatus
US6469285B2 (en) * 2000-06-13 2002-10-22 Shimadzu Corporation Automatic temperature control device
US20040147012A1 (en) * 2002-11-19 2004-07-29 Yasuhiko Yokoi Storage apparatus
US7326565B2 (en) * 2002-11-19 2008-02-05 Sanyo Electric Co., Ltd. Storage apparatus
US20110183367A1 (en) * 2002-12-23 2011-07-28 Lars Ditlev Morck Ottosen Device and method for non-invasive measurement of the individual metabolic rate of a substantially spherical metabolizing particle
US20050115773A1 (en) * 2003-10-27 2005-06-02 Samsung Electronics Co., Ltd. Lift for substrate
US8265357B2 (en) 2005-10-14 2012-09-11 Unisense Fertilitech A/S Determination of a change in a cell population
US20080247628A1 (en) * 2005-10-14 2008-10-09 Unisense Fertilitech A/S Determination of a Change in a Cell Population
US20100041090A1 (en) * 2006-06-16 2010-02-18 Unisense Fertilitech A/S Embryo quality assessment based on blastomere division and movement
US20110165609A1 (en) * 2007-06-29 2011-07-07 Ramsing Niels B Device, a system and a method for monitoring and/or cultivation of microscopic objects
US9588104B2 (en) 2007-06-29 2017-03-07 Unisense Fertilitech A/S Device, a system and a method for monitoring and/or culturing of microscopic objects
US8633017B2 (en) 2007-06-29 2014-01-21 Unisense Fertilitech A/S Device, a system and a method for monitoring and/or cultivation of microscopic objects
WO2010003423A3 (en) * 2008-07-05 2010-08-12 Unisense Fertilitech A/S One to one identification system
WO2010003423A2 (en) * 2008-07-05 2010-01-14 Unisense Fertilitech A/S One to one identification system
US20110111447A1 (en) * 2008-07-05 2011-05-12 Unisense Fertilitech A/S One to one identification system
US20160018429A1 (en) * 2010-09-08 2016-01-21 Tecan Trading Ag Microplate-reader with a controlled gas atmosphere and a corresponding method of controlling the gas atmosphere
US10180441B2 (en) * 2010-09-08 2019-01-15 Tecan Trading Ag Microplate-reader with a controlled gas atmosphere and a corresponding method of controlling the gas atmosphere
US10779513B2 (en) 2013-11-04 2020-09-22 Rose Acre Farms, Inc. Aviary building construction system and method
US10375935B2 (en) * 2013-11-04 2019-08-13 Rose Acre Farms, Inc. Aviary walkway and ventilation system and method of circulating air in an aviary
US10575502B2 (en) 2013-11-04 2020-03-03 Rose Acre Farms, Inc. Aviary walkway and ventilation system and method of circulating air in an aviary
US20150122190A1 (en) * 2013-11-04 2015-05-07 Rose Acre Farms, Inc. Aviary walkway and ventilation system and method of circulating air in an aviary
US11041655B2 (en) 2013-11-04 2021-06-22 Rose Acre Farms, Inc. Aviary ventilation system and method
US11044892B2 (en) 2013-11-04 2021-06-29 Rose Acre Farms, Inc. Aviary system and method of circulating litter in an aviary
GB2554240A (en) * 2015-03-19 2018-03-28 Harvard College Incubating enclosure
WO2016149527A1 (en) * 2015-03-19 2016-09-22 President And Fellows Of Harvard College Incubating enclosure
US11311009B2 (en) 2015-03-19 2022-04-26 President And Fellows Of Harvard College Incubating enclosure
US11185053B2 (en) 2016-07-06 2021-11-30 Rose Acre Farms, Inc. Walkway and walkway system for an aviary

Similar Documents

Publication Publication Date Title
US3618734A (en) Specimen incubator
US7678568B2 (en) Incubator
US4111753A (en) Controlled atmosphere apparatus and method of transferring specimens to same
US7326565B2 (en) Storage apparatus
US4696902A (en) Modular apparatus for cell culture
JP4248512B2 (en) Storage device
US5061448A (en) Incubator
US4572427A (en) Controlled atmosphere enclosure
US3874754A (en) Biohazard safety cabinet
US5169217A (en) Controlled environment chamber apparatus
JP2008113600A (en) Cell culture device and cell culture cassette
US20110189649A1 (en) Laboratory Apparatus for a Controlled Environment
WO2005040330A1 (en) Laboratory apparatus with two cabinets
US20100151564A1 (en) Biological Work Station
DE3924701A1 (en) Microscope incubator - with transparent lid over petri dish heated by warm air circulation
JP4597244B2 (en) incubator
US5507157A (en) Cold storage
CN216614678U (en) Medical science microbial cultivation case that facilitates use
CN211800994U (en) Dustproof detachable biological test-tube rack
US20040152186A1 (en) Culturing apparatus
US3108399A (en) Plant growth cabinets
CN209322908U (en) A kind of drawing and pulling type cell incubator
US2124250A (en) Bacteria plate cabinet
CN217173762U (en) Tumor cell culture device for anti-tumor experiments
CN220564593U (en) Incubator with adjustable temperature