US3355098A - Serum separation apparatus and method - Google Patents
Serum separation apparatus and method Download PDFInfo
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- US3355098A US3355098A US380497A US38049764A US3355098A US 3355098 A US3355098 A US 3355098A US 380497 A US380497 A US 380497A US 38049764 A US38049764 A US 38049764A US 3355098 A US3355098 A US 3355098A
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- plunger
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- nickel
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- 238000000034 method Methods 0.000 title abstract description 10
- 210000002966 serum Anatomy 0.000 title description 28
- 238000000926 separation method Methods 0.000 title description 12
- 239000007788 liquid Substances 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 abstract 6
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 abstract 3
- 229910052744 lithium Inorganic materials 0.000 abstract 3
- 229910052751 metal Inorganic materials 0.000 abstract 3
- 239000002184 metal Substances 0.000 abstract 3
- 229910052759 nickel Inorganic materials 0.000 abstract 3
- 239000004065 semiconductor Substances 0.000 abstract 3
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 abstract 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract 2
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical group ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 abstract 2
- 229910052710 silicon Inorganic materials 0.000 abstract 2
- 239000010703 silicon Substances 0.000 abstract 2
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 abstract 2
- JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 abstract 1
- 229910001218 Gallium arsenide Inorganic materials 0.000 abstract 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 abstract 1
- 239000004411 aluminium Substances 0.000 abstract 1
- 229910052782 aluminium Inorganic materials 0.000 abstract 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 abstract 1
- 239000008367 deionised water Substances 0.000 abstract 1
- 230000008021 deposition Effects 0.000 abstract 1
- 229910052732 germanium Inorganic materials 0.000 abstract 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 abstract 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract 1
- 239000010931 gold Substances 0.000 abstract 1
- 229910052737 gold Inorganic materials 0.000 abstract 1
- 150000002739 metals Chemical class 0.000 abstract 1
- 239000004332 silver Substances 0.000 abstract 1
- 229910052709 silver Inorganic materials 0.000 abstract 1
- 238000010583 slow cooling Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 230000001413 cellular effect Effects 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L21/00—Processes or apparatus adapted for the manufacture or treatment of semiconductor or solid state devices or of parts thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L21/00—Processes or apparatus adapted for the manufacture or treatment of semiconductor or solid state devices or of parts thereof
- H01L21/02—Manufacture or treatment of semiconductor devices or of parts thereof
- H01L21/04—Manufacture or treatment of semiconductor devices or of parts thereof the devices having at least one potential-jump barrier or surface barrier, e.g. PN junction, depletion layer or carrier concentration layer
- H01L21/18—Manufacture or treatment of semiconductor devices or of parts thereof the devices having at least one potential-jump barrier or surface barrier, e.g. PN junction, depletion layer or carrier concentration layer the devices having semiconductor bodies comprising elements of Group IV of the Periodic System or AIIIBV compounds with or without impurities, e.g. doping materials
- H01L21/28—Manufacture of electrodes on semiconductor bodies using processes or apparatus not provided for in groups H01L21/20 - H01L21/268
- H01L21/283—Deposition of conductive or insulating materials for electrodes conducting electric current
- H01L21/288—Deposition of conductive or insulating materials for electrodes conducting electric current from a liquid, e.g. electrolytic deposition
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L29/00—Semiconductor devices adapted for rectifying, amplifying, oscillating or switching, or capacitors or resistors with at least one potential-jump barrier or surface barrier, e.g. PN junction depletion layer or carrier concentration layer; Details of semiconductor bodies or of electrodes thereof ; Multistep manufacturing processes therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Definitions
- This invention relates to the method and apparatus for separation of serum from dispersed solids, particularly blood serum from suspended cells, for the purpose of obtaining samples of clear serum.
- One object of the invention is to provide a method for easy separation of a liquid from suspended solids. Another object is to provide means for easily and quickly separating blood serum from the cellular bodies which have been settled to the bottom portion of a tube, for example as by centrifuging. A further object is to provide inexpensive and disposable apparatus for the removal and storage of the serum from a centrifuged tube of blood. Another object is to provide means for accurately separating one immiscible liquid from another, in a tubular container.
- FIG. 1 is a side elevational view of an assembled serum separation apparatus
- FIG. 2 is a cross-sectional view taken on the line 2-2 of FIG. 1;
- FIG. 3 is a side elevational view of the same in disassembly with parts broken away and shown in section;
- FIG. 4 is a diagrammatic representation of the apparatus in use showing the delivery of serum to the upper chamber of the apparatus for temporary storage therein;
- FIG. 5 is a diagrammatic representation of the apparatus in use showing delivery of serum to another vessel.
- FIG. 6 is a side elevational view of an alternative arrangement with a shortened capillary tube discharging within the plunger tube.
- FIG. 1 there is shown an assembled separation apparatus consisting of an outer tubular member 11, which is generally a sample tube in which the blood sample has been delivered to the laboratory, and in which the blood may be subjected to centrifuging to settle out the cells.
- a plunger tube 12 having a soft rubber piston head 13 attached to its inner end is arranged to be moved longitudinally within and slidingly fitting the inside wall of the member 11, the plunger tube 12 being considerably longer than the tube 11 to provide handle means.
- the outer end 14 of the plunger tube 12 is open, and there is provided within said tube a plastic flexible small bore or capillary tube 15, whose lower end 16 extends through an opening 17 provided in the piston head 13, and whose opposite outer end 18 may extend beyond the open end 14 of'the plunger tube 12, although it may terminate within the 3,355,098 Patented Nov. 28, 1967 plunger tube if desired.
- the outer end portion 18 of the flexible tube 15 may be doubled back into the plunger tube 12 (as shown in FIG. 4) or it may be free to communicate with another receiving vessel 19.
- the method of separating the serum from the suspended solids starts with the centrifuging of the blood sample in the tube 11, following the well known test procedure. After centrifuging, the plunger tube 12 with piston head 13 is inserted in tube 11, the capillary tube 15 being in place with its lower end attached to and penetrating the piston head 13 so that its lower end communicates with the gas or liquid contents of the tube 11 below the piston head 13. As the head 13 is pressed into the tube 11, the air above the serum 20 is released to the atmosphere, and when the end of the capillary tube which extends through the piston head 13 makes contact with the serum 20, the serum passes out through the capillary tube 15, without disturbing the settled out solids or cellular bodies 21.
- the separated serum is collected and retained above the piston head 13 in the plunger tube 12, where it may be kept until required for further examination.
- the outer end 18 of the capillary tube 15 may be extended outside the plunger tube 12, to deliver serum to any desired receiving vessel 19. A portion of serum may be placed in the receiving vessel 19, or several such vessels, and then the remainder of the separated serum may be collected and retained in the plunger tube 12, by doubling back the outer end 18 of the capillary tube 15 into the plunger tube.
- the piston head 13 may be pressed down until all of the separated serum 20 above the settled out solids 21 has been-removed. The portion of the serum which surrounds the cells or solid particles is not removed. Because of the extension of the capillary tube 15 outside the plunger tube 12, the serum in said plunger tube 12 may not return to the tube 11.
- the capillary tube 15A may terminate inside the plunger tube 12 as shown in FIG. 6, in which arrangement the separated serum is collected in the plunger tube 12, from which it may be removed by pouring or in any other convenient manner.
- the plunger tube 12, with piston head 13 and capillary tube 15 may be removed and discarded.
- the tube 11 is generally cleansed and re-used for new blood samples, but if desired, the entire apparatus may be discarded, since the apparatus is very inexpensive, whether made of glass or of synthetic plastic, thus avoiding washing and re-sterilizing.
- the apparatus has other uses. For example, it may be advantageously used to remove a separated top layer in a tube containing immiscible liquids, as sometimes used in liquid extractions, particularly for analytical purposes. It may also be used for removing relatively large amounts of liquid from a settled small body of an analytical precipitate in a tube, in completing well known analytical procedures.
- Word capillary refers to small-bore plastic tubing having a bore of about inch.
- An apparatus for separating a clear liquid from settled-out immiscible liquids and/ or settled-out solids in a suspending liquid contained therein comprising an outer transparent tubular member; a plunger tube longitudinally movable within said outer member; a piston head mounted on the closed inner end of said plunger tube, said piston head making sliding sealing contact with the inner walls of said outer tubular member, and flexible capillary tube disposed within said plunger tube, one end of said capillary tube extending through said piston head to communicate with the space in said outer tubular member below said piston head.
- An apparatus for separating a clear liquid from settled-out immiscible liquids and/or settled-out solids in a suspending liquid contained therein comprising an outer transparent tubular member; a plunger tube longitudinally movable within said outer member; a piston head mounted on the closed inner end of said plunger tube, said piston head making sliding sealing contact with the inner walls of said outer tubular member, and flexible capillary tube disposed within said plunger tube, one end of said capillary tube extending through said piston head to communicate with the space in said outer tubular member below said piston head, said flexible capillary tube extending out of the opposite open end of said plunger tube.
- a plunger tube longitudinally movable within said centrifuge tube; a piston head mounted on the inner end of said plunger tube, said piston head making sliding sealing contact with the inner walls of said outer tubular member, and a flexible capillary tube disposed within said plunger tube, one end of said capillary tube extending through said piston head to communicate with the space in said tubular member below said piston head.
- a flexible capillary tube which extends beyond the outer end of said plunger tube.
- a flexible capillary tube which terminates inside said plunger tube.
Abstract
1,066,165. Semi-conductor devices. DEUTSCHE AKADEMIE DER WISSENSCHAFTEN ZU BERLIN. Aug. 6, 1965, No. 33753/65. Heading H1K. A metal layer is formed on a P-type semiconductor body and lithium is deposited on the layer, the body then being heated to simultaneously temper the metal layer and diffuse lithium into the body to form an N-region and ohmic contact thereon. Suitable semi-conductors are silicon, germanium, and gallium arsenide. Suitable metals are nickel, silver, gold, and aluminium. In an embodiment a 400 ohm. cm. P-type silicon wafer is cleaned ultrasonically in trichloroethylene, etched in hydrofluoric acid, and boiled several times in deionised water before it is nickeled in a hot chemical bath. The nickel is coated with lithium by vapour deposition and the body heated to 600‹ C. After slow cooling the main faces of the wafer are masked and nickel is etched from the edges. The process is completed by a wash in trichloroethylene.
Description
Nov. 28, 1967 A. F. FARR SERUM SEPARATION APPARATUS AND METHOD Filed July 6, 1964 FIG. 5
FIG. 4.
INVENTOR 41701751! IT H I ATTORNEY United States Patent 3,355,098 SERUM SEPARATION APPARATUS AND METHOD Andrew F. Farr, South Gate, Califl, assignor to Bioconsultants, Inc., Glendale, Califi, a corporation of Caiifornia Filed July 6, 1964, Ser. No. 380,497 8 Claims. (Cl. 233-26) This invention relates to the method and apparatus for separation of serum from dispersed solids, particularly blood serum from suspended cells, for the purpose of obtaining samples of clear serum.
One object of the invention is to provide a method for easy separation of a liquid from suspended solids. Another object is to provide means for easily and quickly separating blood serum from the cellular bodies which have been settled to the bottom portion of a tube, for example as by centrifuging. A further object is to provide inexpensive and disposable apparatus for the removal and storage of the serum from a centrifuged tube of blood. Another object is to provide means for accurately separating one immiscible liquid from another, in a tubular container.
These and other objects are attained by my invention which will be understood from the following description, reference being made to the accompanying drawings in which-- FIG. 1 is a side elevational view of an assembled serum separation apparatus;
FIG. 2 is a cross-sectional view taken on the line 2-2 of FIG. 1;
FIG. 3 is a side elevational view of the same in disassembly with parts broken away and shown in section;
FIG. 4 is a diagrammatic representation of the apparatus in use showing the delivery of serum to the upper chamber of the apparatus for temporary storage therein;
FIG. 5 is a diagrammatic representation of the apparatus in use showing delivery of serum to another vessel; and
FIG. 6 is a side elevational view of an alternative arrangement with a shortened capillary tube discharging within the plunger tube.
In the examination of blood samples in a clinical laboratory, it is frequently necessary to examine the blood serum after separation from the suspended cellular material, and for this purpose it is customary to subject the tubes in which the blood samples are delivered to the laboratory, to centrifugal force to cause the settling of the cells to the bottom of the tube. In certain clinical laboratories, hundreds of tube samples of blood are handled per day, and it is important to conserve time and provide completely accurate identification (by attached labels) of each sample both before and after separation of the serum from the solids.
In FIG. 1 there is shown an assembled separation apparatus consisting of an outer tubular member 11, which is generally a sample tube in which the blood sample has been delivered to the laboratory, and in which the blood may be subjected to centrifuging to settle out the cells. A plunger tube 12 having a soft rubber piston head 13 attached to its inner end is arranged to be moved longitudinally within and slidingly fitting the inside wall of the member 11, the plunger tube 12 being considerably longer than the tube 11 to provide handle means. The outer end 14 of the plunger tube 12 is open, and there is provided within said tube a plastic flexible small bore or capillary tube 15, whose lower end 16 extends through an opening 17 provided in the piston head 13, and whose opposite outer end 18 may extend beyond the open end 14 of'the plunger tube 12, although it may terminate within the 3,355,098 Patented Nov. 28, 1967 plunger tube if desired. The outer end portion 18 of the flexible tube 15 may be doubled back into the plunger tube 12 (as shown in FIG. 4) or it may be free to communicate with another receiving vessel 19.
The method of separating the serum from the suspended solids starts with the centrifuging of the blood sample in the tube 11, following the well known test procedure. After centrifuging, the plunger tube 12 with piston head 13 is inserted in tube 11, the capillary tube 15 being in place with its lower end attached to and penetrating the piston head 13 so that its lower end communicates with the gas or liquid contents of the tube 11 below the piston head 13. As the head 13 is pressed into the tube 11, the air above the serum 20 is released to the atmosphere, and when the end of the capillary tube which extends through the piston head 13 makes contact with the serum 20, the serum passes out through the capillary tube 15, without disturbing the settled out solids or cellular bodies 21. If the outer end 18 of the capillary tube is doubled back to reenter the plunger tube 12, as shown in FIG. 4, the separated serum is collected and retained above the piston head 13 in the plunger tube 12, where it may be kept until required for further examination. Alternatively, as shown in FIG. 5, the outer end 18 of the capillary tube 15 may be extended outside the plunger tube 12, to deliver serum to any desired receiving vessel 19. A portion of serum may be placed in the receiving vessel 19, or several such vessels, and then the remainder of the separated serum may be collected and retained in the plunger tube 12, by doubling back the outer end 18 of the capillary tube 15 into the plunger tube. The piston head 13 may be pressed down until all of the separated serum 20 above the settled out solids 21 has been-removed. The portion of the serum which surrounds the cells or solid particles is not removed. Because of the extension of the capillary tube 15 outside the plunger tube 12, the serum in said plunger tube 12 may not return to the tube 11.
In an alternative arrangement, the capillary tube 15A may terminate inside the plunger tube 12 as shown in FIG. 6, in which arrangement the separated serum is collected in the plunger tube 12, from which it may be removed by pouring or in any other convenient manner.
After the separation has been completed, and the retained separated serum is no longer needed, the plunger tube 12, with piston head 13 and capillary tube 15 may be removed and discarded. The tube 11 is generally cleansed and re-used for new blood samples, but if desired, the entire apparatus may be discarded, since the apparatus is very inexpensive, whether made of glass or of synthetic plastic, thus avoiding washing and re-sterilizing.
While the use of the apparatus has been described with reference to the separation and collection of blood serum, the apparatus has other uses. For example, it may be advantageously used to remove a separated top layer in a tube containing immiscible liquids, as sometimes used in liquid extractions, particularly for analytical purposes. It may also be used for removing relatively large amounts of liquid from a settled small body of an analytical precipitate in a tube, in completing well known analytical procedures.
Where the descriptive Word capillary is used herein it refers to small-bore plastic tubing having a bore of about inch.
The advantages of the method and use of the apparatus will be apparent from the above description. The saving in handling time for making the separation, and the avoid ance of re-labeling of the temporarily stored serum (avoiding the danger of mislabeling) make the invention useful in analytical examinations.
The objectives stated in the beginning have been attained.
I claim:
1. An apparatus for separating a clear liquid from settled-out immiscible liquids and/ or settled-out solids in a suspending liquid contained therein, comprising an outer transparent tubular member; a plunger tube longitudinally movable within said outer member; a piston head mounted on the closed inner end of said plunger tube, said piston head making sliding sealing contact with the inner walls of said outer tubular member, and flexible capillary tube disposed within said plunger tube, one end of said capillary tube extending through said piston head to communicate with the space in said outer tubular member below said piston head.
2. An apparatus for separating a clear liquid from settled-out immiscible liquids and/or settled-out solids in a suspending liquid contained therein, comprising an outer transparent tubular member; a plunger tube longitudinally movable within said outer member; a piston head mounted on the closed inner end of said plunger tube, said piston head making sliding sealing contact with the inner walls of said outer tubular member, and flexible capillary tube disposed within said plunger tube, one end of said capillary tube extending through said piston head to communicate with the space in said outer tubular member below said piston head, said flexible capillary tube extending out of the opposite open end of said plunger tube.
3. The apparatus defined in claim 2 in which the outer end of the capillary tube is doubled back so that it communicates with said plunger tube.
4. In an apparatus for separating a clear liquid from separated out solids, contained in a centrifuge tube, a plunger tube longitudinally movable within said centrifuge tube; a piston head mounted on the inner end of said plunger tube, said piston head making sliding sealing contact with the inner walls of said outer tubular member, and a flexible capillary tube disposed within said plunger tube, one end of said capillary tube extending through said piston head to communicate with the space in said tubular member below said piston head.
5. In the plunger tube defined in claim 4, a flexible capillary tube which extends beyond the outer end of said plunger tube.
6. In the plunger tube defined in claim 4, a flexible capillary tube which terminates inside said plunger tube.
5 7. The method of removing a separated lighter liquid from a heavier material contained in the bottom portion of a first zone comprising:
(a) Applying mechanical pressure by pushing tubular plunger means against the surface of the overlaying liquid whereby the end portion means of said plunger means decreases the volume of said bottom portion of said first zone;
(b) Relieving said pressure by displacing said lighter liquid from substantially the surface portion thereof through orifice means in said end portion of said plunger means;
(0) Moving said displaced liquid from said surface through conduit means disposed internally of said plunger means and distinct from the tubular wall of said plunger means to a point displaced from said surface contacted by said plunger means;
(d) Then flowing said moved liquid into a second zone.
8. The method of claim 7 wherein the second zone is within the bottom portion of said tubular plunger means.
References Cited UNITED STATES PATENTS 2,362,081 11/1944 Martin 103-188 2,394,189 2/1964 Kaufman 103188 X 3,131,646 5/1964 Parrott 103--188 X 1,591,604 7/1926 Bienenstok 222-320 1,714,482 5/1929 Schmuziger 222320 FOREIGN PATENTS 1,888 1/1893 Great Britain.
OTHER REFERENCES German printed application 1,014,348, August 1957.
HENRY T. KLINKSIEK, Primary Examiner.
M. CARY NELSON, Examiner.
Claims (1)
1. AN APPARATUS FOR SEPARATING A CLEAR LIQUID FROM SETTLED-OUT IMMISCIBLE LIQUIDS AND/OR SETTLED-OUT SOLIDS IN A SUSPENDING LIQUID CONTAINED THEREIN, COMPRISING AN OUTER TRANSPARENT TUBULAR MEMBER; A PLUNGER TUBE LONGITUDINALLY MOVABLE WITHIN SAID OUTER MEMBER; A PISTON HEAD MOUNTED ON THE CLOSED INNER END OF SAID PLUNGER TUBE, SAID PISTON HEAD MAKING SLIDING SEALING CONTACT WITH THE INNER WALLS OF SAID OUTER TUBULAR MEMBER, AND FLEXIBLE CAPILLARY TUBE DISPOSED WITHIN SAID PLUNGER TUBE, ONE END OF SAID CAPILLARY TUBE EXTENDING THROUGH SAID PISTON HEAD TO COMMUNICATE WITH THE SPACE IN SAID OUTER TUBULAR MEMBER BELOW SAID PISTON HEAD.
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US380497A US3355098A (en) | 1964-07-06 | 1964-07-06 | Serum separation apparatus and method |
GB25703/65A GB1066164A (en) | 1964-07-06 | 1965-06-17 | Serum separation apparatus and method |
DE19651598053 DE1598053B2 (en) | 1964-07-06 | 1965-06-25 | DEVICE FOR SEPARATING LIQUID FROM DISPERSED SOLIDS |
GB33753/65A GB1066165A (en) | 1964-07-06 | 1965-08-06 | Process for the production of barrier layer elements |
JP4786469A JPS542693B1 (en) | 1964-07-06 | 1969-06-16 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US380497A US3355098A (en) | 1964-07-06 | 1964-07-06 | Serum separation apparatus and method |
GB33753/65A GB1066165A (en) | 1964-07-06 | 1965-08-06 | Process for the production of barrier layer elements |
Publications (1)
Publication Number | Publication Date |
---|---|
US3355098A true US3355098A (en) | 1967-11-28 |
Family
ID=26261999
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US380497A Expired - Lifetime US3355098A (en) | 1964-07-06 | 1964-07-06 | Serum separation apparatus and method |
Country Status (4)
Country | Link |
---|---|
US (1) | US3355098A (en) |
JP (1) | JPS542693B1 (en) |
DE (1) | DE1598053B2 (en) |
GB (2) | GB1066164A (en) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3508653A (en) * | 1967-11-17 | 1970-04-28 | Charles M Coleman | Method and apparatus for fluid handling and separation |
US3651945A (en) * | 1970-03-16 | 1972-03-28 | Lilly Co Eli | Continuous culture separation apparatus |
US3661265A (en) * | 1970-07-27 | 1972-05-09 | Contemporary Research And Dev | Serum separator type container |
JPS4933690A (en) * | 1972-07-22 | 1974-03-28 | ||
US3905528A (en) * | 1974-04-10 | 1975-09-16 | Hugh V Maiocco | Two-piece concentric centrifuge sample container |
US3983037A (en) * | 1973-11-05 | 1976-09-28 | Jae Yoon Lee | Apparatus for transfer, storage, and distribution of liquid |
JPS51115368A (en) * | 1975-04-02 | 1976-10-09 | Sanki Eng Kk | Method and apparatus for continuously centrifugally separating |
US4003834A (en) * | 1975-03-31 | 1977-01-18 | The United States Of America As Represented By The Secretary Of The Department Of Health, Education And Welfare | Density gradient fractionation by piston displacement |
US4046699A (en) * | 1976-11-01 | 1977-09-06 | Corning Glass Works | Access device for centrifugal separation assemblies |
US4142668A (en) * | 1976-10-01 | 1979-03-06 | Lee Jae Y | Serum-plasma separator and transfer apparatus |
DE3046979A1 (en) * | 1979-12-12 | 1981-10-01 | Michael Prof. Cais | METHOD AND DEVICE FOR MASS TRANSFER OPERATIONS IN IMMUNOASSAY AND OTHER APPLICATIONS |
US4326959A (en) * | 1981-02-04 | 1982-04-27 | Ferrara Louis T | Blood separator and dispenser |
WO1984002004A1 (en) * | 1982-11-15 | 1984-05-24 | Quidel | Integrated single tube plunger immunoassay system |
US4487696A (en) * | 1978-08-14 | 1984-12-11 | Ferrara Louis T | Blood separator and dispenser |
WO1985002260A1 (en) * | 1983-11-08 | 1985-05-23 | Quidel | Rapid plunger immunoassay method and apparatus |
US5550060A (en) * | 1992-11-03 | 1996-08-27 | Chronomed, Inc. | Method and procedure for preparing red blood fractions |
US5980734A (en) * | 1996-05-09 | 1999-11-09 | Itoh; Teruaki | Auxiliary apparatus for sampling blood serum |
US20040256331A1 (en) * | 2002-10-04 | 2004-12-23 | Arking E. James | System and method for fractionation of a centrifuged sample |
EP1773494A1 (en) * | 2004-05-28 | 2007-04-18 | Stephen Clark Wardlaw | Specimen analysis tube |
US11786894B2 (en) | 2020-10-29 | 2023-10-17 | Ashim Gupta | Whole blood separator device and method of use |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4022576A (en) * | 1975-06-09 | 1977-05-10 | I. C. L. Scientific | Method and apparatus for preparation of liquids containing suspended material for examination |
IL60645A (en) * | 1980-07-21 | 1984-02-29 | Cais Michael | Method and device for mass transfer and separation through selective barriers |
JPS61110276U (en) * | 1984-12-25 | 1986-07-12 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US1591604A (en) * | 1921-10-31 | 1926-07-06 | Milwaukee Tank Works | Grease distributor |
US1714482A (en) * | 1927-05-28 | 1929-05-21 | Schmuziger Willy | Discharging device for paints and the like |
US2362081A (en) * | 1943-04-26 | 1944-11-07 | Wilco Company | Pump unit for spray or jet devices |
US2394189A (en) * | 1942-10-06 | 1946-02-05 | Texaco Development Corp | Control of paraffin deposition |
US3131646A (en) * | 1962-10-15 | 1964-05-05 | Parco Products Co | Hand pump |
-
1964
- 1964-07-06 US US380497A patent/US3355098A/en not_active Expired - Lifetime
-
1965
- 1965-06-17 GB GB25703/65A patent/GB1066164A/en not_active Expired
- 1965-06-25 DE DE19651598053 patent/DE1598053B2/en not_active Withdrawn
- 1965-08-06 GB GB33753/65A patent/GB1066165A/en not_active Expired
-
1969
- 1969-06-16 JP JP4786469A patent/JPS542693B1/ja active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US1591604A (en) * | 1921-10-31 | 1926-07-06 | Milwaukee Tank Works | Grease distributor |
US1714482A (en) * | 1927-05-28 | 1929-05-21 | Schmuziger Willy | Discharging device for paints and the like |
US2394189A (en) * | 1942-10-06 | 1946-02-05 | Texaco Development Corp | Control of paraffin deposition |
US2362081A (en) * | 1943-04-26 | 1944-11-07 | Wilco Company | Pump unit for spray or jet devices |
US3131646A (en) * | 1962-10-15 | 1964-05-05 | Parco Products Co | Hand pump |
Cited By (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3508653A (en) * | 1967-11-17 | 1970-04-28 | Charles M Coleman | Method and apparatus for fluid handling and separation |
US3651945A (en) * | 1970-03-16 | 1972-03-28 | Lilly Co Eli | Continuous culture separation apparatus |
US3661265A (en) * | 1970-07-27 | 1972-05-09 | Contemporary Research And Dev | Serum separator type container |
JPS4933690A (en) * | 1972-07-22 | 1974-03-28 | ||
US3983037A (en) * | 1973-11-05 | 1976-09-28 | Jae Yoon Lee | Apparatus for transfer, storage, and distribution of liquid |
US3905528A (en) * | 1974-04-10 | 1975-09-16 | Hugh V Maiocco | Two-piece concentric centrifuge sample container |
US4003834A (en) * | 1975-03-31 | 1977-01-18 | The United States Of America As Represented By The Secretary Of The Department Of Health, Education And Welfare | Density gradient fractionation by piston displacement |
JPS51115368A (en) * | 1975-04-02 | 1976-10-09 | Sanki Eng Kk | Method and apparatus for continuously centrifugally separating |
JPS541057B2 (en) * | 1975-04-02 | 1979-01-19 | ||
US4142668A (en) * | 1976-10-01 | 1979-03-06 | Lee Jae Y | Serum-plasma separator and transfer apparatus |
US4046699A (en) * | 1976-11-01 | 1977-09-06 | Corning Glass Works | Access device for centrifugal separation assemblies |
US4487696A (en) * | 1978-08-14 | 1984-12-11 | Ferrara Louis T | Blood separator and dispenser |
DE3046979A1 (en) * | 1979-12-12 | 1981-10-01 | Michael Prof. Cais | METHOD AND DEVICE FOR MASS TRANSFER OPERATIONS IN IMMUNOASSAY AND OTHER APPLICATIONS |
US4326959A (en) * | 1981-02-04 | 1982-04-27 | Ferrara Louis T | Blood separator and dispenser |
US4458020A (en) * | 1982-11-15 | 1984-07-03 | Quidel | Integrated single tube plunger immunoassay system having plural reagent chambers |
WO1984002004A1 (en) * | 1982-11-15 | 1984-05-24 | Quidel | Integrated single tube plunger immunoassay system |
WO1985002260A1 (en) * | 1983-11-08 | 1985-05-23 | Quidel | Rapid plunger immunoassay method and apparatus |
US5550060A (en) * | 1992-11-03 | 1996-08-27 | Chronomed, Inc. | Method and procedure for preparing red blood fractions |
US5980734A (en) * | 1996-05-09 | 1999-11-09 | Itoh; Teruaki | Auxiliary apparatus for sampling blood serum |
US20040256331A1 (en) * | 2002-10-04 | 2004-12-23 | Arking E. James | System and method for fractionation of a centrifuged sample |
US20100238287A1 (en) * | 2002-10-04 | 2010-09-23 | Ikonisys, Inc. | System and method for fractionation of a centrifuged sample |
EP1773494A1 (en) * | 2004-05-28 | 2007-04-18 | Stephen Clark Wardlaw | Specimen analysis tube |
EP1773494A4 (en) * | 2004-05-28 | 2009-06-03 | Wardlaw Stephen C | Specimen analysis tube |
US11786894B2 (en) | 2020-10-29 | 2023-10-17 | Ashim Gupta | Whole blood separator device and method of use |
Also Published As
Publication number | Publication date |
---|---|
JPS542693B1 (en) | 1979-02-10 |
GB1066164A (en) | 1967-04-19 |
DE1598053B2 (en) | 1972-01-27 |
DE1598053A1 (en) | 1971-07-29 |
GB1066165A (en) | 1967-04-19 |
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