US20130003062A1 - Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism - Google Patents
Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism Download PDFInfo
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- US20130003062A1 US20130003062A1 US13/593,180 US201213593180A US2013003062A1 US 20130003062 A1 US20130003062 A1 US 20130003062A1 US 201213593180 A US201213593180 A US 201213593180A US 2013003062 A1 US2013003062 A1 US 2013003062A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- This invention relates to scientific instruments and methods, and more particularly to surface plasmon resonance spectroscopy.
- SPR Surface Plasmon Resonance
- SPR microscopy utilizes an angle of incidence of the irradiating beam at the prime SPR angle so that the system is conditioned to operate at its maximum linear response region.
- the procedure then involves rotating both sample and/or the detector and light source to establish the optimum optical pass configuration.
- Fine resolution rotation tables or linear diode arrays have been employed to provide the angular scanning function to obtain the SPR reflecting signal dip.
- Fixed wavelength, coherent angle scanning SPR employing dual rotation tables generally involves instruments having the optical pass configured in the horizontal plane. The physical size required for rotation stages offering fine resolution and providing enough torque to support the swing arms that hold either light source and/or detector gives the SPR instrument a large footprint. Thus, there is a need for an SPR instrument having a reduced footprint that allows SPR angle scanning.
- One embodiment is an SPR spectrometer comprising a semi-circular rail and a driving mechanism, wherein the driving mechanism is attached to a light source mount and a detector mount, and wherein both the light source mount and the detector mount are attached to the semi-circular rail with connectors, each connectors allowing the light source mount and detector mount to slide along the rail.
- one embodiment is an instrument, comprising: a semicircular rail ( 2 ); a sample stage for receiving a sample ( 14 ), the sample stage forming a plane; a light source mount ( 8 ) on the rail ( 2 ); a light source ( 8 a ) on the light source mount ( 8 ); a detector mount ( 10 ) on the rail ( 2 ); a detector ( 10 a ) on the detector mount ( 10 ), wherein the light source mount ( 8 ) and the detector mount ( 10 ) move synchronously along the rail ( 2 ) in opposite directions ( 11 a , 11 b ).
- the synchronous movement of the light source mount ( 8 ) and the detector mount ( 10 ) changes the angle of incidence of a light beam ( 12 ) from the light source ( 8 a ) with respect to the plane of the sample surface on the sample stage ( 14 ).
- the instrument further comprises a driving mechanism that comprises, referring to FIG. 2 : a driving bridge ( 3 ) having a first pivot point ( 4 a ) and a second pivot point ( 6 a ); a first swing arm ( 4 ) with a first end ( 4 b ) and a second end ( 4 c ), the first end ( 4 b ) being connected to the driving bridge ( 3 ) through the first pivot point ( 4 a ); and a second swing arm ( 6 ) with a first end ( 6 b ) and a second end ( 6 c ), the first end ( 6 b ) being connected to the driving bridge ( 3 ) through the second pivot point ( 6 a ), wherein the second end ( 4 c ) of the first swing arm ( 4 ) is connected to a pivot point on the light source mount ( 8 b ) and the second end ( 6 c ) of the second swing arm ( 6 ) is connected to a pivot point on the detector mount ( 10 b ).
- a driving mechanism
- the driving bridge ( 3 ) moves along a path ( 15 ) substantially perpendicular to the plane of the sample stage, the light source mount ( 8 ) and the detector mount ( 10 ) move in opposite directions ( 11 a and 11 b ).
- Using a single actuator to move the driving mechanism significantly reduces the instrument's physical size and mechanical complexity needed when, for example, dual rotation tables are used.
- Another embodiment is a method, comprising: 1) providing a light source, a detector, and a sample, wherein the light source generates a light beam; 2) directing the light beam at the sample to form and angle of incidence between the light beam and the sample; and 3) moving the light source and the detector synchronously by sliding the light source and detector in opposite directions along a semicircular rail, thereby modifying the angle of incidence.
- the sample is a microarray comprising gold and the light beam generates surface plasmon resonance at the gold surface.
- FIG. 1 illustrates one embodiment
- FIG. 2 illustrates another embodiment that includes a driving mechanism.
- FIG. 3 illustrates the movement of some components in FIG. 2 .
- FIG. 4 is a plot of a surface plasmon resonance signal while modifying the angle of incidence.
- one embodiment is an instrument, comprising: a semicircular rail ( 2 ); a sample stage for receiving a sample ( 14 ), the sample stage ( 14 ) forming a plane on which a sample may be placed; a light source mount ( 8 ) on the rail ( 2 ); a light source ( 8 a ) on the light source mount ( 8 ); a detector mount ( 10 ) on the rail ( 2 ); a detector ( 10 a ) on the detector mount ( 10 ), wherein the light source mount ( 8 ) and the detector mount ( 10 ) move synchronously along the rail ( 2 ) in opposite directions (denoted by arrows 11 a and 11 b ).
- the synchronous movement of the light source mount ( 8 ) and the detector mount ( 10 ) changes the angle of incidence of a light beam ( 12 ) from the light source ( 8 a ) with respect to the plane of the sample surface on the sample stage ( 14 ).
- the sample stage ( 14 ) may be used for a microarray sample comprising gold, for example.
- the sample stage ( 14 ) may further include a microfluidic flow cell for supplying a liquid analyte to the surface of the microarray, and temperature regulator that may be used to influence instrument sensitivity by suppressing thermally induced sample changes in refractive index.
- the instrument further comprises a driving mechanism that comprises, referring to FIG. 2 : a driving bridge ( 3 ) having a first pivot point ( 4 a ) and a second pivot point ( 6 a ); a first swing arm ( 4 ) with a first end ( 4 b ) and a second end ( 4 c ), the first end ( 4 b ) being connected to the driving bridge ( 3 ) through the first pivot point ( 4 a ); and a second swing arm ( 6 ) with a first end ( 6 b ) and a second end ( 6 c ), the first end ( 6 b ) being connected to the driving bridge ( 3 ) through the second pivot point ( 6 a ), wherein the second end ( 4 c ) of the first swing arm ( 4 ) is connected to a pivot point on the light source mount ( 8 b ) and the second end ( 6 c ) of the second swing arm ( 6 ) is connected to a pivot point on the detector mount ( 10 b ).
- a driving mechanism
- the movement of the driving bridge ( 3 ) is effected by a linear actuator.
- the light source ( 8 a ) comprises a laser that generates a laser beam.
- the laser beam is scanned across the surface of the sample with a microelectromechanical (MEMS) scanner.
- MEMS microelectromechanical
- the MEMS scanner can use a micromirror to reflect and manipulate the light beam path, for example see U.S. Pat. Nos. 6,245,590; 6,362,912; 6,433,907; and 5,629,790.
- the laser operates at wavelengths from about 360 nm to about 2000 nm.
- the detector ( 10 a ) is a CCD camera.
- the instrument further comprises a prism assembly mounted beneath the sample stage ( 14 ).
- a prism in the prism assembly is located at the bottom of the sample.
- the prism assembly and the sample are made of materials with similar refractive indices and are coupled to each other with an index-matching fluid.
- Light from the light source ( 8 a ) passes through one face of the prism, passes through the face of the prism that is coupled to the substrate of the microarray, and reflects off the sample surface (e.g., a gold surface).
- the reflected light again passes through the face of the prism coupled to the sample substrate, passes through a third face of the prism, and impinges on the detector ( 10 a ).
- the sample plane is roughly perpendicular to the plane of the semi-circular rail ( 2 ).
- the first swing arm ( 4 ) and the second swing arm ( 6 ) may be curved.
- the amount of curvature can depend on many factors including, for example, the distance between the sample ( 14 ) and the light source mount ( 8 ), the corresponding curvature of the rail ( 2 ), and the location of the pivot points ( 4 b , 4 c , 6 b , and 6 c ).
- Each of the light source mount ( 8 ) and the detector mount ( 10 ) can rest, for example, on the semicircular rail ( 2 ) through at least two wheels.
- the light source mount ( 8 ) may further include a polarizer.
- the instrument includes a mirror assembly.
- the mirror assembly can provide flexibility in placing the light source ( 8 a ) on the light source mount ( 8 ).
- the detector mount ( 10 ) further includes a telescope in the light path ( 12 ) between the sample ( 14 ) and the detector ( 10 a ).
- Another embodiment is a method, comprising: providing a light source, a detector, and a sample, wherein the light source generates a light beam; directing the light beam at the sample thereby forming an angle of incidence between the light beam and the sample; and moving the light source and the detector substantially synchronously by sliding the light source and detector in opposite directions along a semicircular rail, thereby modifying the angle of incidence.
- the sample is a microarray comprising gold and the light beam generates a surface plasmon at the gold surface. Methods and systems for producing microarrays on gold are well known.
- Microarrays of, for example, nucleic acids, peptides, or proteins covalently or noncovalently bound to a thiol monolayer can be produced on the surface of a gold substrate.
- the spots on the microarray maybe separated from each other, for example, by hydrophobic areas in cases where the spots are hydrophilic.
- the detector is a CCD camera having pixels.
- One pixel may correspond, for example, to a single spot on the microarray to give a pixel-spot assignment, wherein the pixel-spot assignment does not change as the angle of incidence is modified.
- a group of pixels of the CCD camera may correspond to a single spot on the microarray, forming a pixel group-spot assignment, wherein the pixel group-spot assignment does not change as the angle of incidence is modified.
- at least one linear actuator controls the sliding of the light source and the detector along the semicircular rail.
- the light source can be a laser that forms a laser beam.
- the light beam is scanned across the surface of the sample with a frequency.
- the light beam may be scanned, for example, by using a MEMS scanner as described above.
- the rate at which the light source and the detector slide along the rail may be, for example, slower than the frequency of the scan rate such that sample is scanned at least once before the angle of incidence is substantially modified. This means that the detector can be exposed to one or more full scans before the angle of incidence is modified.
- the light source can include a laser capable of producing light at different wavelengths, for example, from 360 nm to 2000 nm.
- the light source is mounted on a light source mount; the detector is mounted on a detector mount; a first swing arm connects the light mount to a driving bridge; a second swing arm connects the detector mount to the driving bridge, and one linear actuator moves the driving bridge in a path perpendicular to a plane where the sample resides.
- the method comprises: scanning a region on the microarray to be used in an assay; plotting the intensity of light at the detector against the magnitude of the displacement of the linear actuator to give a curve comprising a linear slope ( 50 in (FIG.
- the point is near the bottom of the linear slope ( 52 ).
Abstract
Instruments and methods relating to surface plasmon imaging are described. An instrument comprises a semi-circular rail and a driving mechanism. The driving mechanism is attached to a light source mount and a detector mount, and both the light source mount and the detector mount are attached to the semi-circular rail with connectors. Each connector allows the light source mount and detector mount to slide along the rail. The synchronous movement of the light source mount and the detector mount changes the angle of incidence of a light beam from the light source with respect to the plane of the sample surface on the sample stage.
Description
- This application is a continuation of and claims priority to U.S. patent application Ser. No. 12/958,125 filed Dec. 1, 2010 which is a continuation of and claims priority to U.S. patent application Ser. No. 11/562,197 filed Nov. 21, 2006 which issued as U.S. Pat. No. 7,889,347 and which claims priority to U.S. Provisional Patent Application Ser. No. 60/738,880, filed on Nov. 21, 2005, the entire contents of which are hereby expressly incorporated by reference in their entireties.
- This invention relates to scientific instruments and methods, and more particularly to surface plasmon resonance spectroscopy.
- All patents, patent applications, and publications cited within this application are incorporated herein by reference to the same extent as if each individual patent, patent application or publication was specifically and individually incorporated by reference.
- Surface Plasmon Resonance (SPR) spectroscopy is a powerful method capable of detecting molecular binding events at the nanometer scale by detecting changes in the effective refractive index or thickness of an adsorbed layer on or near an SPR active surface. When light is reflected from an SPR active medium at an angle greater than the critical angle, incident photons can generate surface plasmons. This phenomenon can be observed as a function of the reflected light intensity. The spatial difference of contrast can be acquired in an image format by employing a CCD camera as a detection system, namely SPR microscopy (SPRM).
- Typically, SPR microscopy utilizes an angle of incidence of the irradiating beam at the prime SPR angle so that the system is conditioned to operate at its maximum linear response region. The procedure then involves rotating both sample and/or the detector and light source to establish the optimum optical pass configuration. Fine resolution rotation tables or linear diode arrays have been employed to provide the angular scanning function to obtain the SPR reflecting signal dip. Fixed wavelength, coherent angle scanning SPR employing dual rotation tables generally involves instruments having the optical pass configured in the horizontal plane. The physical size required for rotation stages offering fine resolution and providing enough torque to support the swing arms that hold either light source and/or detector gives the SPR instrument a large footprint. Thus, there is a need for an SPR instrument having a reduced footprint that allows SPR angle scanning.
- One embodiment is an SPR spectrometer comprising a semi-circular rail and a driving mechanism, wherein the driving mechanism is attached to a light source mount and a detector mount, and wherein both the light source mount and the detector mount are attached to the semi-circular rail with connectors, each connectors allowing the light source mount and detector mount to slide along the rail. Referring to
FIG. 1 , one embodiment is an instrument, comprising: a semicircular rail (2); a sample stage for receiving a sample (14), the sample stage forming a plane; a light source mount (8) on the rail (2); a light source (8 a) on the light source mount (8); a detector mount (10) on the rail (2); a detector (10 a) on the detector mount (10), wherein the light source mount (8) and the detector mount (10) move synchronously along the rail (2) in opposite directions (11 a, 11 b). The synchronous movement of the light source mount (8) and the detector mount (10) changes the angle of incidence of a light beam (12) from the light source (8 a) with respect to the plane of the sample surface on the sample stage (14). - In another embodiment, the instrument further comprises a driving mechanism that comprises, referring to
FIG. 2 : a driving bridge (3) having a first pivot point (4 a) and a second pivot point (6 a); a first swing arm (4) with a first end (4 b) and a second end (4 c), the first end (4 b) being connected to the driving bridge (3) through the first pivot point (4 a); and a second swing arm (6) with a first end (6 b) and a second end (6 c), the first end (6 b) being connected to the driving bridge (3) through the second pivot point (6 a), wherein the second end (4 c) of the first swing arm (4) is connected to a pivot point on the light source mount (8 b) and the second end (6 c) of the second swing arm (6) is connected to a pivot point on the detector mount (10 b). Referring toFIGS. 2 and 3 , when the driving bridge (3) moves along a path (15) substantially perpendicular to the plane of the sample stage, the light source mount (8) and the detector mount (10) move in opposite directions (11 a and 11 b). Using a single actuator to move the driving mechanism significantly reduces the instrument's physical size and mechanical complexity needed when, for example, dual rotation tables are used. - Another embodiment is a method, comprising: 1) providing a light source, a detector, and a sample, wherein the light source generates a light beam; 2) directing the light beam at the sample to form and angle of incidence between the light beam and the sample; and 3) moving the light source and the detector synchronously by sliding the light source and detector in opposite directions along a semicircular rail, thereby modifying the angle of incidence. In another embodiment, the sample is a microarray comprising gold and the light beam generates surface plasmon resonance at the gold surface.
- The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims.
-
FIG. 1 illustrates one embodiment. -
FIG. 2 illustrates another embodiment that includes a driving mechanism. -
FIG. 3 illustrates the movement of some components inFIG. 2 . -
FIG. 4 is a plot of a surface plasmon resonance signal while modifying the angle of incidence. - Like reference symbols in the various drawings indicate like elements.
- Referring to
FIG. 1 , one embodiment is an instrument, comprising: a semicircular rail (2); a sample stage for receiving a sample (14), the sample stage (14) forming a plane on which a sample may be placed; a light source mount (8) on the rail (2); a light source (8 a) on the light source mount (8); a detector mount (10) on the rail (2); a detector (10 a) on the detector mount (10), wherein the light source mount (8) and the detector mount (10) move synchronously along the rail (2) in opposite directions (denoted byarrows - In another embodiment, the instrument further comprises a driving mechanism that comprises, referring to
FIG. 2 : a driving bridge (3) having a first pivot point (4 a) and a second pivot point (6 a); a first swing arm (4) with a first end (4 b) and a second end (4 c), the first end (4 b) being connected to the driving bridge (3) through the first pivot point (4 a); and a second swing arm (6) with a first end (6 b) and a second end (6 c), the first end (6 b) being connected to the driving bridge (3) through the second pivot point (6 a), wherein the second end (4 c) of the first swing arm (4) is connected to a pivot point on the light source mount (8 b) and the second end (6 c) of the second swing arm (6) is connected to a pivot point on the detector mount (10 b). Referring toFIGS. 2 and 3 , when the driving bridge (3) moves along a path (15) substantially perpendicular to the plane of the sample stage (14), the light source mount (8) and the detector mount (10) move in opposite directions (denoted byarrows FIG. 1 ). - In one embodiment, the movement of the driving bridge (3) is effected by a linear actuator. In another embodiment, the light source (8 a) comprises a laser that generates a laser beam. In many embodiments, the laser beam is scanned across the surface of the sample with a microelectromechanical (MEMS) scanner. The MEMS scanner can use a micromirror to reflect and manipulate the light beam path, for example see U.S. Pat. Nos. 6,245,590; 6,362,912; 6,433,907; and 5,629,790. In one embodiment the laser operates at wavelengths from about 360 nm to about 2000 nm. In many embodiments, the detector (10 a) is a CCD camera. In other embodiments, the instrument further comprises a prism assembly mounted beneath the sample stage (14).
- During operation in such a configuration, a prism in the prism assembly is located at the bottom of the sample. The prism assembly and the sample (e.g., a microarray substrate) are made of materials with similar refractive indices and are coupled to each other with an index-matching fluid. Light from the light source (8 a) passes through one face of the prism, passes through the face of the prism that is coupled to the substrate of the microarray, and reflects off the sample surface (e.g., a gold surface). The reflected light again passes through the face of the prism coupled to the sample substrate, passes through a third face of the prism, and impinges on the detector (10 a).
- In most embodiments, the sample plane is roughly perpendicular to the plane of the semi-circular rail (2). The first swing arm (4) and the second swing arm (6) may be curved. The amount of curvature can depend on many factors including, for example, the distance between the sample (14) and the light source mount (8), the corresponding curvature of the rail (2), and the location of the pivot points (4 b, 4 c, 6 b, and 6 c). Each of the light source mount (8) and the detector mount (10) can rest, for example, on the semicircular rail (2) through at least two wheels. The light source mount (8) may further include a polarizer. In some embodiments, the instrument includes a mirror assembly. The mirror assembly can provide flexibility in placing the light source (8 a) on the light source mount (8). In other embodiments, the detector mount (10) further includes a telescope in the light path (12) between the sample (14) and the detector (10 a).
- Another embodiment is a method, comprising: providing a light source, a detector, and a sample, wherein the light source generates a light beam; directing the light beam at the sample thereby forming an angle of incidence between the light beam and the sample; and moving the light source and the detector substantially synchronously by sliding the light source and detector in opposite directions along a semicircular rail, thereby modifying the angle of incidence. In one embodiment of the method, the sample is a microarray comprising gold and the light beam generates a surface plasmon at the gold surface. Methods and systems for producing microarrays on gold are well known. Microarrays of, for example, nucleic acids, peptides, or proteins covalently or noncovalently bound to a thiol monolayer can be produced on the surface of a gold substrate. The spots on the microarray maybe separated from each other, for example, by hydrophobic areas in cases where the spots are hydrophilic. In many embodiments of the method, the detector is a CCD camera having pixels. One pixel may correspond, for example, to a single spot on the microarray to give a pixel-spot assignment, wherein the pixel-spot assignment does not change as the angle of incidence is modified. Alternatively, a group of pixels of the CCD camera may correspond to a single spot on the microarray, forming a pixel group-spot assignment, wherein the pixel group-spot assignment does not change as the angle of incidence is modified. In another embodiment of the method, at least one linear actuator controls the sliding of the light source and the detector along the semicircular rail.
- In all embodiments, the light source can be a laser that forms a laser beam. In many embodiments, the light beam is scanned across the surface of the sample with a frequency. The light beam may be scanned, for example, by using a MEMS scanner as described above. When the light beam is scanned, the rate at which the light source and the detector slide along the rail may be, for example, slower than the frequency of the scan rate such that sample is scanned at least once before the angle of incidence is substantially modified. This means that the detector can be exposed to one or more full scans before the angle of incidence is modified. In many embodiments the light source can include a laser capable of producing light at different wavelengths, for example, from 360 nm to 2000 nm.
- In many embodiments, the light source is mounted on a light source mount; the detector is mounted on a detector mount; a first swing arm connects the light mount to a driving bridge; a second swing arm connects the detector mount to the driving bridge, and one linear actuator moves the driving bridge in a path perpendicular to a plane where the sample resides. In another embodiment, the method comprises: scanning a region on the microarray to be used in an assay; plotting the intensity of light at the detector against the magnitude of the displacement of the linear actuator to give a curve comprising a linear slope (50 in (FIG. 4)); choosing a specific point on the linear slope; moving the linear actuator to the displacement corresponding to the specific point to give a fixed angle of incidence; and performing the assay at the fixed angle of incidence. In many embodiments, referring to
FIG. 4 , the point is near the bottom of the linear slope (52). - A number of embodiments of the invention have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the invention. Accordingly, other embodiments are within the scope of the following claims.
Claims (20)
1. An instrument comprising:
a rail, where the rail traverses a portion of the perimeter of a circle;
a slidable light source associated with the rail;
a swing arm to locate the position of the light source on the rail;
a sample stage forming a plane adapted to generate surface plasmons when irradiated by the light source;
a slidable detector associated with the rail adapted to detect changes in light intensity; and
a linear actuator to adjust the position of the light source and the detector to an optimum optical pass configuration, where the position of the light source and the position of the detector are moved synchronously in opposite directions along the rail to the optimum optical pass configuration.
2. The instrument of claim 1 , where the angle of incidence of the light source on the sample stage is varied to determine the optimum optical pass configuration.
3. The instrument of claim 1 , where the optimum optical pass configuration is chosen such that light from the source directed at the sample stage is optimally reflected at an angle less than the critical angle to generate surface plasmons.
4. The instrument of claim 1 , where the optimum optical pass configuration is based at least in part on the effective refractive index of the sample stage.
5. The instrument of claim 1 , further comprising a micromirror located at one or both the sample stage and the detector.
6. The instrument of claim 1 , further comprising a telescope tube located at the detector.
7. The instrument of claim 1 , where the detector is a CCD camera.
8. The instrument of claim 1 , where the sample stage is positioned roughly perpendicular to the plane of the rail.
9. The instrument of claim 1 , where the light source is a laser.
10. The instrument of claim 9 , further comprising a rotating mirror assembly to scan the laser.
11. The instrument of claim 1 , further comprising a prism positioned to alter the light emitted by the light source.
12. The instrument of claim 11 , where the optimum optical pass configuration is based at least in part on optimizing the refractive index at the wavelength of the light of the prism.
13. The instrument of claim 11 , where the prism and the sample stage are made of materials with similar refractive indices.
14. The instrument of claim 11 , where the prism and the sample stage are coupled to each other with an index-matching substance.
15. The instrument of claim 11 , where light from the light source passes through one face of the prism, passes through the prism and is reflected off the sample surface coupled to the prism, exits the third face of the prism and impinges on the detector.
16. The instrument of claim 1 , further comprising one or more light polarizers positioned to alter the light emitted by the light source.
17. The instrument of claim 1 , further comprising one or more wave plates positioned to alter the light emitted by the light source.
18. An instrument comprising:
a rail, where the rail traverses a portion of the perimeter of a circle;
a slidable light source associated with the rail;
a first swing arm to locate the position of the light source on the rail;
a sample stage forming a plane adapted to generate surface plasmons when irradiated by the light source;
a slidable detector adapted to detect changes in light intensity associated with the rail;
a second swing arm to locate the position of the slidable detector on the rail; and
a linear actuator to adjust the position of the light source and the slidable detector to an optimum optical pass configuration, where the first and second swing arms are connected to the linear actuator, where the position of the light source and the position of the detector are moved synchronously in opposite directions along the rail to the optimum optical pass configuration.
19. The instrument of claim 17 , where one or both the first and second swing arms are curved.
20. An instrument comprising:
a rail, where the rail traverses a portion of the perimeter of a circle;
a light emitting diode (LED) associated with the rail;
a swing arm to locate the position of the LED on the rail;
a sample stage adapted to generate surface plasmons when irradiated by the LED;
a slidable detector associated with the rail adapted to detect changes in light intensity; and
a linear actuator to adjust the position of the LED and the slidable detector to an optimum optical pass configuration, where the position of the LED and the position of the detector are moved synchronously in opposite directions along the rail to the optimum optical pass configuration.
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US13/593,180 US20130003062A1 (en) | 2005-11-21 | 2012-08-23 | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
US14/155,116 US20140185051A1 (en) | 2005-11-21 | 2014-01-14 | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
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US73888005P | 2005-11-21 | 2005-11-21 | |
US11/562,197 US7889347B2 (en) | 2005-11-21 | 2006-11-21 | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
US12/958,125 US8264691B2 (en) | 2005-11-21 | 2010-12-01 | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
US13/593,180 US20130003062A1 (en) | 2005-11-21 | 2012-08-23 | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
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US13/593,180 Abandoned US20130003062A1 (en) | 2005-11-21 | 2012-08-23 | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
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US20040258832A1 (en) * | 2003-06-17 | 2004-12-23 | Barklund Anna M. | Method of chemical analysis using microwells patterned from self-assembled monolayers and substrates |
US7745143B2 (en) | 2004-11-19 | 2010-06-29 | Plexera, Llc | Plasmon resonance biosensor and method |
US20090262356A1 (en) * | 2008-03-27 | 2009-10-22 | Plexera, Llc | User interface and method for using an spr system |
US7889347B2 (en) | 2005-11-21 | 2011-02-15 | Plexera Llc | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
US7463358B2 (en) | 2005-12-06 | 2008-12-09 | Lumera Corporation | Highly stable surface plasmon resonance plates, microarrays, and methods |
US7473916B2 (en) * | 2005-12-16 | 2009-01-06 | Asml Netherlands B.V. | Apparatus and method for detecting contamination within a lithographic apparatus |
US8263377B2 (en) | 2007-04-03 | 2012-09-11 | Plexera, Llc | Label free kinase assays and reagents |
US7695976B2 (en) * | 2007-08-29 | 2010-04-13 | Plexera Bioscience, Llc | Method for uniform analyte fluid delivery to microarrays |
US20090060786A1 (en) * | 2007-08-29 | 2009-03-05 | Gibum Kim | Microfluidic apparatus for wide area microarrays |
GB0721482D0 (en) | 2007-11-01 | 2007-12-12 | Univ Exeter | Plasmon resonance based sensor |
US8004669B1 (en) | 2007-12-18 | 2011-08-23 | Plexera Llc | SPR apparatus with a high performance fluid delivery system |
US8199507B2 (en) * | 2008-12-19 | 2012-06-12 | Openpeak Inc. | Telephony and digital media services device |
WO2014089120A1 (en) | 2012-12-07 | 2014-06-12 | Integrated Plasmonics Corporation | Plasmonic spectroscopic sensor and cuvette therefor |
US9976963B2 (en) | 2012-12-21 | 2018-05-22 | Integrated Plasmonics Corporation | Microcuvette cartridge |
US10481089B2 (en) | 2013-03-12 | 2019-11-19 | Integrated Plasmonics Corporation | Optical detection system with tilted sensor |
US9784986B2 (en) | 2013-03-14 | 2017-10-10 | Integrated Plasmonics Corporation | Self-aligned spatial filter |
WO2014143235A1 (en) | 2013-03-14 | 2014-09-18 | Integrated Plasmonics Corporation | Ambient light assisted spectroscopy |
KR101490921B1 (en) * | 2013-07-11 | 2015-02-06 | 현대자동차 주식회사 | Quality inspecting device of automotive parts |
CN108318451A (en) * | 2018-05-07 | 2018-07-24 | 中国计量大学 | A kind of solid material refractive index measuring instrument from motion tracking reflection light |
CN109115727A (en) * | 2018-08-08 | 2019-01-01 | 河南农业大学 | A kind of surface plasma resonance biological sensing detection device of multichannel |
TWI685252B (en) * | 2018-08-15 | 2020-02-11 | 友達光電股份有限公司 | Scenario projection system and controlling method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4542963A (en) * | 1984-03-28 | 1985-09-24 | The United States Of America As Represented By The United States National Aeronautics And Space Administration | Optical system with reflective baffles |
US5641640A (en) * | 1992-06-29 | 1997-06-24 | Biacore Ab | Method of assaying for an analyte using surface plasmon resonance |
US20050200845A1 (en) * | 2004-03-11 | 2005-09-15 | Nataliya Nabatova-Gabain | Measuring method, analyzing method, measuring apparatus, analyzing apparatus, ellipsometer, and computer program |
US20070109542A1 (en) * | 2003-08-01 | 2007-05-17 | Tracy David H | Optical resonance analysis unit |
US7312069B2 (en) * | 2003-12-26 | 2007-12-25 | Matsushita Electric Industrial Co., Ltd. | Method of analyzing ligand in sample and apparatus for analyzing ligand in sample |
Family Cites Families (177)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3105902A (en) * | 1960-09-19 | 1963-10-01 | Standard Oil Co | Controlled atmosphere X-ray diffraction spectrometer |
US3751587A (en) | 1972-01-20 | 1973-08-07 | Saxon Ind Inc | Laser printing system |
US3891507A (en) | 1974-05-30 | 1975-06-24 | American Cyanamid Co | Organ function test cards |
US4038030A (en) | 1975-04-10 | 1977-07-26 | American Hospital Supply Corporation | Profile analysis pack and method |
US3990850A (en) | 1976-01-06 | 1976-11-09 | Akzona Incorporated | Diagnostic test card |
US4148057A (en) | 1977-10-25 | 1979-04-03 | Solution Sciences, Inc. | Direct laser printing and forming apparatus |
US4375025A (en) | 1980-06-19 | 1983-02-22 | Automated Industrial Systems, Inc. | Laser strip marker |
US4585931A (en) | 1983-11-21 | 1986-04-29 | At&T Technologies, Inc. | Method for automatically identifying semiconductor wafers |
GB8417301D0 (en) | 1984-07-06 | 1984-08-08 | Serono Diagnostics Ltd | Assay |
US4707722A (en) | 1984-12-17 | 1987-11-17 | Motorola, Inc. | Laser marking method and ablative coating for use therein |
US4753863A (en) | 1985-02-01 | 1988-06-28 | Motorola Inc. | Laser markable molding compound |
US4740468A (en) | 1985-02-14 | 1988-04-26 | Syntex (U.S.A.) Inc. | Concentrating immunochemical test device and method |
US4638144A (en) | 1985-04-24 | 1987-01-20 | Automated Industrial Systems | Indexing laser marker |
JPS6234920A (en) | 1985-08-07 | 1987-02-14 | Toshiba Corp | Epoxy resin composition and resin-encapsulated semiconductor device produced by using same |
EP0215669A3 (en) | 1985-09-17 | 1989-08-30 | Seiko Instruments Inc. | Analytical device and method for analysis of biochemicals, microbes and cells |
KR910000826B1 (en) | 1986-11-14 | 1991-02-09 | 미쓰비시덴기 가부시기가이샤 | Method of laser marking |
US5192507A (en) | 1987-06-05 | 1993-03-09 | Arthur D. Little, Inc. | Receptor-based biosensors |
EP0382736B1 (en) | 1987-07-27 | 1994-11-02 | Commonwealth Scientific And Industrial Research Organisation | Receptor membranes |
CA1321488C (en) * | 1987-08-22 | 1993-08-24 | Martin Francis Finlan | Biological sensors |
DE3731835A1 (en) | 1987-09-22 | 1989-03-30 | Siemens Ag | LASER BEAM INDUCED COLOR PRINTING |
US5078855A (en) | 1987-10-13 | 1992-01-07 | Taiyo Yuden Co., Ltd. | Chemical sensors and their divided parts |
US6054270A (en) | 1988-05-03 | 2000-04-25 | Oxford Gene Technology Limited | Analying polynucleotide sequences |
SE462408B (en) | 1988-11-10 | 1990-06-18 | Pharmacia Ab | OPTICAL BIOSENSOR SYSTEM USING SURFACE MONITORING RESONSE FOR THE DETECTION OF A SPECIFIC BIOMOLIC CYCLE, TO CALIBRATE THE SENSOR DEVICE AND TO CORRECT FOUND BASELINE OPERATION IN THE SYSTEM |
JPH02133185A (en) | 1988-11-10 | 1990-05-22 | Mitsubishi Electric Corp | Laser marking method for semiconductor device |
SE8804074D0 (en) | 1988-11-10 | 1988-11-10 | Pharmacia Ab | SENSOR UNIT AND ITS USE IN BIOSENSOR SYSTEM |
SE462454B (en) | 1988-11-10 | 1990-06-25 | Pharmacia Ab | METHOD FOR USE IN BIOSENSORS |
US5200051A (en) | 1988-11-14 | 1993-04-06 | I-Stat Corporation | Wholly microfabricated biosensors and process for the manufacture and use thereof |
US5089112A (en) | 1989-03-20 | 1992-02-18 | Associated Universities, Inc. | Electrochemical biosensor based on immobilized enzymes and redox polymers |
DK0418355T3 (en) | 1989-04-04 | 1994-09-12 | Urban Gerald | Method for immobilizing proteins, peptides, coenzymes, etc. on a substrate |
DE59002516D1 (en) | 1989-04-06 | 1993-10-07 | Ciba Geigy | Laser marking of ceramic materials, glazes, ceramic glasses and glasses. |
US5116481A (en) | 1989-04-07 | 1992-05-26 | Hitachi, Ltd. | Anion-selective, sensitive film, electrode containing the same and the use thereof |
US6416952B1 (en) | 1989-06-07 | 2002-07-09 | Affymetrix, Inc. | Photolithographic and other means for manufacturing arrays |
US6346413B1 (en) | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5268305A (en) | 1989-06-15 | 1993-12-07 | Biocircuits Corporation | Multi-optical detection system |
US5491097A (en) | 1989-06-15 | 1996-02-13 | Biocircuits Corporation | Analyte detection with multilayered bioelectronic conductivity sensors |
US5368712A (en) | 1989-11-02 | 1994-11-29 | Synporin Technologies, Inc. | Biologically mimetic synthetic ion channel transducers |
US5068124A (en) | 1989-11-17 | 1991-11-26 | International Business Machines Corporation | Method for depositing high quality silicon dioxide by pecvd |
US5104619A (en) | 1990-01-24 | 1992-04-14 | Gds Technology, Inc. | Disposable diagnostic system |
GB9005872D0 (en) | 1990-03-15 | 1990-05-09 | British Aerospace | A laser markable white pigment composition |
US5478756A (en) | 1990-07-24 | 1995-12-26 | Fisons Plc | Chemical sensor for detecting binding reactions |
DE69130962T2 (en) | 1990-11-07 | 1999-10-28 | Teijin Ltd | Polyester resin composition |
DE69132843T2 (en) | 1990-12-06 | 2002-09-12 | Affymetrix Inc N D Ges D Staat | Identification of nucleic acids in samples |
DE69232641T2 (en) | 1991-03-27 | 2003-02-20 | Ambri Ltd | IONIC RESERVOIR ON THE SURFACE OF AN ELECTRODE |
US6111645A (en) * | 1991-04-29 | 2000-08-29 | Massachusetts Institute Of Technology | Grating based phase control optical delay line |
US5474796A (en) | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
JP2862413B2 (en) | 1991-10-02 | 1999-03-03 | ポリプラスチックス株式会社 | Laser marking method |
DE69233331T3 (en) | 1991-11-22 | 2007-08-30 | Affymetrix, Inc., Santa Clara | Combinatorial Polymersynthesis Strategies |
US5756355A (en) | 1992-04-22 | 1998-05-26 | Ecole Polytechnique Federale De Lausanne | Lipid membrane sensors |
JP3102822B2 (en) | 1992-05-29 | 2000-10-23 | 日本ジーイープラスチックス株式会社 | Resin composition for laser marking |
US5736410A (en) | 1992-09-14 | 1998-04-07 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
US5445923A (en) | 1992-09-30 | 1995-08-29 | Somar Corporation | Laser beam absorbing resin composition and laser beam marking method |
ATE210731T1 (en) | 1992-10-01 | 2001-12-15 | Au Membrane & Biotech Res Inst | IMPROVED SENSOR MEMBRANES |
JP2751089B2 (en) | 1992-11-30 | 1998-05-18 | 大日本インキ化学工業株式会社 | Laser marking method and printing ink |
JPH0810729B2 (en) | 1993-01-20 | 1996-01-31 | 日本電気株式会社 | Stamping machine |
DE4303860C2 (en) | 1993-02-10 | 1995-11-09 | Draegerwerk Ag | Carrier for colorimetric gas detection in composite film construction |
US5329090A (en) | 1993-04-09 | 1994-07-12 | A B Lasers, Inc. | Writing on silicon wafers |
US5512492A (en) | 1993-05-18 | 1996-04-30 | University Of Utah Research Foundation | Waveguide immunosensor with coating chemistry providing enhanced sensitivity |
NZ267842A (en) | 1993-05-28 | 1997-09-22 | Baylor College Medicine | Apparatus for measuring molecular mass (by mass spectrometry) in which the sample is ionised on the sample holder and desorbed therefrom by laser pulses |
US6020208A (en) | 1994-05-27 | 2000-02-01 | Baylor College Of Medicine | Systems for surface-enhanced affinity capture for desorption and detection of analytes |
GB9315847D0 (en) | 1993-07-30 | 1993-09-15 | Isis Innovation | Tag reagent and assay method |
WO1995006240A1 (en) | 1993-08-24 | 1995-03-02 | Metrika Laboratories, Inc. | Novel disposable electronic assay device |
US5512131A (en) | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
US5629790A (en) | 1993-10-18 | 1997-05-13 | Neukermans; Armand P. | Micromachined torsional scanner |
US5470952A (en) | 1993-10-20 | 1995-11-28 | Regeneron Pharmaceuticals, Inc. | CNTF and IL-6 antagonists |
US5527711A (en) | 1993-12-13 | 1996-06-18 | Hewlett Packard Company | Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic techniques |
IL108726A (en) | 1994-02-22 | 1999-12-31 | Yissum Res Dev Co | Electrobiochemical method and system for the determination of an analyte which is a member of a recognition pair in a liquid medium and electrodes therefor |
US5536822A (en) | 1994-03-09 | 1996-07-16 | University Of Virginia Alumni Patents Foundation | γ-phosphate linked adenosine 5' triphosphate sepharose |
FR2720832A1 (en) | 1994-04-22 | 1995-12-08 | Francis Garnier | Electroactive electrodes and membranes based on bioactive peptides, for the recognition, extraction or release of biologically active species. |
US5824483A (en) | 1994-05-18 | 1998-10-20 | Pence Inc. | Conformationally-restricted combinatiorial library composition and method |
US5514501A (en) | 1994-06-07 | 1996-05-07 | The United States Of America As Represented By The Secretary Of Commerce | Process for UV-photopatterning of thiolate monolayers self-assembled on gold, silver and other substrates |
US6287850B1 (en) | 1995-06-07 | 2001-09-11 | Affymetrix, Inc. | Bioarray chip reaction apparatus and its manufacture |
CA2152756A1 (en) | 1994-06-28 | 1995-12-29 | Tadakazu Yamauchi | Method and device for specific binding assay |
US5485277A (en) | 1994-07-26 | 1996-01-16 | Physical Optics Corporation | Surface plasmon resonance sensor and methods for the utilization thereof |
DE4430023A1 (en) | 1994-08-24 | 1996-02-29 | Boehringer Mannheim Gmbh | Electrochemical sensor |
US5624537A (en) | 1994-09-20 | 1997-04-29 | The University Of British Columbia - University-Industry Liaison Office | Biosensor and interface membrane |
SE9403245D0 (en) | 1994-09-26 | 1994-09-26 | Pharmacia Biosensor Ab | Improvements relating to bilayer lipid membranes |
US5688642A (en) | 1994-12-01 | 1997-11-18 | The United States Of America As Represented By The Secretary Of The Navy | Selective attachment of nucleic acid molecules to patterned self-assembled surfaces |
US5567301A (en) | 1995-03-01 | 1996-10-22 | Illinois Institute Of Technology | Antibody covalently bound film immunobiosensor |
US5798030A (en) | 1995-05-17 | 1998-08-25 | Australian Membrane And Biotechnology Research Institute | Biosensor membranes |
US5690894A (en) | 1995-05-23 | 1997-11-25 | The Regents Of The University Of California | High density array fabrication and readout method for a fiber optic biosensor |
AUPN366995A0 (en) | 1995-06-20 | 1995-07-13 | Australian Membrane And Biotechnology Research Institute | Self-assembly of bilayer membrane sensors |
IT1275482B (en) | 1995-07-05 | 1997-08-07 | Cooperativa Centro Ricerche Po | ELECTROCHEMICAL BIOSENSORS BASED ON COMPOSITE TRANSDUCERS |
US5838361A (en) | 1996-01-11 | 1998-11-17 | Micron Technology, Inc. | Laser marking techniques |
GB9602542D0 (en) | 1996-02-08 | 1996-04-10 | Fisons Plc | Analytical device |
US6165335A (en) | 1996-04-25 | 2000-12-26 | Pence And Mcgill University | Biosensor device and method |
US5955379A (en) | 1996-04-25 | 1999-09-21 | Mcgill University | Biosensor device and method |
US5938595A (en) | 1996-05-24 | 1999-08-17 | The Regents Of The University Of California | Fiber optic D dimer biosensor |
US5707502A (en) | 1996-07-12 | 1998-01-13 | Chiron Diagnostics Corporation | Sensors for measuring analyte concentrations and methods of making same |
US5832165A (en) | 1996-08-28 | 1998-11-03 | University Of Utah Research Foundation | Composite waveguide for solid phase binding assays |
US6024925A (en) | 1997-01-23 | 2000-02-15 | Sequenom, Inc. | Systems and methods for preparing low volume analyte array elements |
US6379929B1 (en) | 1996-11-20 | 2002-04-30 | The Regents Of The University Of Michigan | Chip-based isothermal amplification devices and methods |
SE9700384D0 (en) | 1997-02-04 | 1997-02-04 | Biacore Ab | Analytical method and apparatus |
US6406845B1 (en) | 1997-05-05 | 2002-06-18 | Trustees Of Tuft College | Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample |
NZ516848A (en) | 1997-06-20 | 2004-03-26 | Ciphergen Biosystems Inc | Retentate chromatography apparatus with applications in biology and medicine |
US6245506B1 (en) | 1997-07-30 | 2001-06-12 | Bbi Bioseq, Inc. | Integrated sequencing device |
US6207370B1 (en) | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
US5922617A (en) | 1997-11-12 | 1999-07-13 | Functional Genetics, Inc. | Rapid screening assay methods and devices |
US6101946A (en) | 1997-11-21 | 2000-08-15 | Telechem International Inc. | Microarray printing device including printing pins with flat tips and exterior channel and method of manufacture |
AU751280B2 (en) | 1997-12-12 | 2002-08-08 | Applera Corporation | Optical resonance analysis system |
US6232066B1 (en) | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
US6074616A (en) | 1998-01-05 | 2000-06-13 | Biosite Diagnostics, Inc. | Media carrier for an assay device |
US6565813B1 (en) | 1998-02-04 | 2003-05-20 | Merck & Co., Inc. | Virtual wells for use in high throughput screening assays |
AU2586799A (en) | 1998-02-06 | 1999-08-23 | Affymetrix, Inc. | Method of quality control in manufacturing processes |
US6150147A (en) | 1998-02-06 | 2000-11-21 | Affymetrix, Inc. | Biological array fabrication methods with reduction of static charge |
US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
US6197599B1 (en) | 1998-07-30 | 2001-03-06 | Guorong Chin | Method to detect proteins |
US6937221B2 (en) | 1998-08-05 | 2005-08-30 | Microvision, Inc. | Scanned beam display |
US6380365B1 (en) | 1998-09-04 | 2002-04-30 | Affitech As | Temperature dependent ligand facilitated purification of proteins |
FR2783179B1 (en) | 1998-09-16 | 2000-10-06 | Commissariat Energie Atomique | CHEMICAL OR BIOLOGICAL ANALYSIS DEVICE COMPRISING A PLURALITY OF ANALYSIS SITES ON A MEDIUM, AND ITS MANUFACTURING METHOD |
US6472179B2 (en) | 1998-09-25 | 2002-10-29 | Regeneron Pharmaceuticals, Inc. | Receptor based antagonists and methods of making and using |
AU1717600A (en) | 1998-11-10 | 2000-05-29 | Biocrystal Limited | Methods for identification and verification |
WO2000052456A1 (en) | 1999-03-02 | 2000-09-08 | Helix Biopharma Corporation | Biosensor device and method |
US6489102B2 (en) | 1999-08-05 | 2002-12-03 | Wisconsin Alumni Research Foundation | Biomolecule and/or cellular arrays on metal surfaces and product produced thereby |
US6245590B1 (en) | 1999-08-05 | 2001-06-12 | Microvision Inc. | Frequency tunable resonant scanner and method of making |
US6433907B1 (en) | 1999-08-05 | 2002-08-13 | Microvision, Inc. | Scanned display with plurality of scanning assemblies |
US6362912B1 (en) | 1999-08-05 | 2002-03-26 | Microvision, Inc. | Scanned imaging apparatus with switched feeds |
US6558623B1 (en) | 2000-07-06 | 2003-05-06 | Robodesign International, Inc. | Microarray dispensing with real-time verification and inspection |
US6448089B1 (en) | 1999-10-12 | 2002-09-10 | Aurora Biosciences Corporation | Multiwell scanner and scanning method |
US6219138B1 (en) | 2000-01-10 | 2001-04-17 | The United States Of America As Represented By The Secretary Of The Navy | Particle sizing technique |
WO2001064710A2 (en) | 2000-02-29 | 2001-09-07 | Progenics Pharmaceuticals, Inc. | Sulfated ccr5 peptides for hiv-1 infection |
US6489106B1 (en) | 2000-03-10 | 2002-12-03 | Nec Research Institute, Inc. | Control of the expression of anchored genes using micron scale heaters |
US6447723B1 (en) | 2000-03-13 | 2002-09-10 | Packard Instrument Company, Inc. | Microarray spotting instruments incorporating sensors and methods of using sensors for improving performance of microarray spotting instruments |
AU2001242927A1 (en) | 2000-03-14 | 2001-09-24 | Institutet Polymerutveckling Ab | Improved imaging spr apparatus |
WO2001070861A2 (en) | 2000-03-17 | 2001-09-27 | Dow Global Technologies Inc. | Polyolefin foam for sound and thermal insulation |
US6806361B1 (en) | 2000-03-17 | 2004-10-19 | Affymetrix, Inc. | Methods of enhancing functional performance of nucleic acid arrays |
US7126688B2 (en) | 2000-07-11 | 2006-10-24 | Maven Technologies, Llc | Microarray scanning |
DE50115382D1 (en) | 2000-08-09 | 2010-04-22 | Artificial Sensing Instr Asi A | WAVEGUIDE STRUCTURE AND OPTICAL MEASURING ARRANGEMENT |
CA2419490C (en) | 2000-08-15 | 2010-01-26 | Discerna Limited | Functional protein arrays |
US6545758B1 (en) | 2000-08-17 | 2003-04-08 | Perry Sandstrom | Microarray detector and synthesizer |
US6789040B2 (en) | 2000-08-22 | 2004-09-07 | Affymetrix, Inc. | System, method, and computer software product for specifying a scanning area of a substrate |
AU2002213043A1 (en) | 2000-10-06 | 2002-04-15 | Protasis Corporation | Fluid separation conduit cartridge |
US7118710B2 (en) | 2000-10-30 | 2006-10-10 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
DE10064146A1 (en) | 2000-12-22 | 2002-07-04 | Andreas Hofmann | Biosensor and method for its production |
WO2002059601A1 (en) | 2001-01-23 | 2002-08-01 | President And Fellows Of Harvard College | Nucleic-acid programmable protein arrays |
EP1370690B1 (en) | 2001-03-16 | 2012-03-14 | Kalim Mir | Arrays and methods of use |
US6885454B2 (en) | 2001-03-28 | 2005-04-26 | Fuji Photo Film Co., Ltd. | Measuring apparatus |
US6862398B2 (en) | 2001-03-30 | 2005-03-01 | Texas Instruments Incorporated | System for directed molecular interaction in surface plasmon resonance analysis |
US20020197729A1 (en) | 2001-06-21 | 2002-12-26 | Fuji Photo Film Co., Ltd. | Biochemical analysis unit and method for manufacturing the same |
US6485918B1 (en) | 2001-07-02 | 2002-11-26 | Packard Bioscience Corporation | Method and apparatus for incubation of a liquid reagent and target spots on a microarray substrate |
GB0119062D0 (en) | 2001-08-06 | 2001-09-26 | Cambridge Consultants | Interferometer |
WO2003018797A2 (en) | 2001-08-22 | 2003-03-06 | Helix Biopharma Corporation | Method and device for integrated protein expression, purification and detection |
WO2003027652A1 (en) * | 2001-09-21 | 2003-04-03 | Olympus Corporation | Defect inspection apparatus |
US7300798B2 (en) | 2001-10-18 | 2007-11-27 | Agilent Technologies, Inc. | Chemical arrays |
AU2002365404A1 (en) | 2001-11-27 | 2003-06-10 | Compound Therapeutics, Inc. | Solid-phase immobilization of proteins and peptides |
GR1004178B (en) | 2001-11-29 | 2003-03-05 | "����������" | Integrated optoelectronic silicon biosensor for the detection of biomolecules labeled with chromophore groups or nanoparticles |
KR20030047567A (en) | 2001-12-11 | 2003-06-18 | 한국전자통신연구원 | Surface plasmon resonance sensor system |
WO2003054524A1 (en) | 2001-12-11 | 2003-07-03 | Sau Lan Tang Staats | Microfluidic devices and methods for two-dimensional separations |
US20040048311A1 (en) | 2002-01-24 | 2004-03-11 | Dana Ault-Riche | Use of collections of binding sites for sample profiling and other applications |
US20040014946A1 (en) | 2002-04-25 | 2004-01-22 | Heman Chao | Protein interaction method and composition |
US7064827B2 (en) | 2002-05-20 | 2006-06-20 | Brown University Research Foundation | Optical tracking and detection of particles by solid state energy sources |
EP1526373B1 (en) | 2002-06-21 | 2013-11-27 | Olympus Corporation | Biomolecule analyzer |
US20040067597A1 (en) | 2002-07-31 | 2004-04-08 | Caliper Technologies Corp. | High density reagent array preparation methods |
US20040043384A1 (en) | 2002-08-28 | 2004-03-04 | Oleinikov Andrew V. | In vitro protein translation microarray device |
EP1583816A4 (en) | 2002-12-22 | 2007-06-13 | Scripps Research Inst | Protein arrays |
EP1587840B1 (en) | 2003-01-13 | 2008-08-20 | The Regents Of The University Of Michigan | Method to form a protein microarray system |
JP3856763B2 (en) | 2003-03-11 | 2006-12-13 | 財団法人川村理化学研究所 | Manufacturing method of microfluidic device |
JP3775677B2 (en) | 2003-05-07 | 2006-05-17 | 船井電機株式会社 | MEMS mirror device and optical disk device |
US7373255B2 (en) | 2003-06-06 | 2008-05-13 | Biacore Ab | Method and system for determination of molecular interaction parameters |
US20040258832A1 (en) | 2003-06-17 | 2004-12-23 | Barklund Anna M. | Method of chemical analysis using microwells patterned from self-assembled monolayers and substrates |
WO2005019811A2 (en) | 2003-08-26 | 2005-03-03 | Blueshift Biotechnologies, Inc. | Time dependent fluorescence measurements |
US20050095577A1 (en) | 2003-10-31 | 2005-05-05 | Yang Dan-Hui D. | Protein bioarray on silane-modified substrate surface |
US20070009198A1 (en) | 2004-07-02 | 2007-01-11 | Robert Petcavich | Fiber optic bio-sensor |
US7745143B2 (en) | 2004-11-19 | 2010-06-29 | Plexera, Llc | Plasmon resonance biosensor and method |
US7445887B2 (en) | 2005-01-07 | 2008-11-04 | Fortebio, Inc. | Enzyme activity measurements using bio-layer interferometry |
KR100668323B1 (en) | 2005-01-19 | 2007-01-12 | 삼성전자주식회사 | Portable biochip scanner using surface plasmon resonance |
US20060234265A1 (en) | 2005-03-21 | 2006-10-19 | Jim Richey | Microarrays having multi-functional, compartmentalized analysis areas and methods of use |
US20070081163A1 (en) | 2005-06-03 | 2007-04-12 | Minhua Liang | Method and apparatus for scanned beam microarray assay |
US20070139653A1 (en) | 2005-06-07 | 2007-06-21 | Guan Hann W | MEMS Micromirror Surface Plasmon Resonance Biosensor and Method |
JP4671346B2 (en) | 2005-09-13 | 2011-04-13 | キヤノン株式会社 | Biochemical reaction cassette with improved liquid filling |
US7889347B2 (en) | 2005-11-21 | 2011-02-15 | Plexera Llc | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
US7463358B2 (en) | 2005-12-06 | 2008-12-09 | Lumera Corporation | Highly stable surface plasmon resonance plates, microarrays, and methods |
US20070140918A1 (en) | 2005-12-19 | 2007-06-21 | Hongfeng Yin | Fluidic separation devices and methods with reduced sample broadening |
US7695976B2 (en) | 2007-08-29 | 2010-04-13 | Plexera Bioscience, Llc | Method for uniform analyte fluid delivery to microarrays |
US20090060786A1 (en) | 2007-08-29 | 2009-03-05 | Gibum Kim | Microfluidic apparatus for wide area microarrays |
-
2006
- 2006-11-21 US US11/562,197 patent/US7889347B2/en active Active
- 2006-11-21 WO PCT/US2006/044957 patent/WO2007061981A2/en active Application Filing
-
2010
- 2010-12-01 US US12/958,125 patent/US8264691B2/en active Active
-
2012
- 2012-08-23 US US13/593,180 patent/US20130003062A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4542963A (en) * | 1984-03-28 | 1985-09-24 | The United States Of America As Represented By The United States National Aeronautics And Space Administration | Optical system with reflective baffles |
US5641640A (en) * | 1992-06-29 | 1997-06-24 | Biacore Ab | Method of assaying for an analyte using surface plasmon resonance |
US20070109542A1 (en) * | 2003-08-01 | 2007-05-17 | Tracy David H | Optical resonance analysis unit |
US7312069B2 (en) * | 2003-12-26 | 2007-12-25 | Matsushita Electric Industrial Co., Ltd. | Method of analyzing ligand in sample and apparatus for analyzing ligand in sample |
US20050200845A1 (en) * | 2004-03-11 | 2005-09-15 | Nataliya Nabatova-Gabain | Measuring method, analyzing method, measuring apparatus, analyzing apparatus, ellipsometer, and computer program |
Also Published As
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US20070222996A1 (en) | 2007-09-27 |
US7889347B2 (en) | 2011-02-15 |
WO2007061981A2 (en) | 2007-05-31 |
US20110085167A1 (en) | 2011-04-14 |
US8264691B2 (en) | 2012-09-11 |
WO2007061981A3 (en) | 2009-06-04 |
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