US20110159549A1 - Temperature control for light-emitting diode stabilization - Google Patents
Temperature control for light-emitting diode stabilization Download PDFInfo
- Publication number
- US20110159549A1 US20110159549A1 US12/895,848 US89584810A US2011159549A1 US 20110159549 A1 US20110159549 A1 US 20110159549A1 US 89584810 A US89584810 A US 89584810A US 2011159549 A1 US2011159549 A1 US 2011159549A1
- Authority
- US
- United States
- Prior art keywords
- led
- temperature
- operating temperature
- various embodiments
- leds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000006641 stabilisation Effects 0.000 title description 7
- 238000011105 stabilization Methods 0.000 title description 7
- 230000005284 excitation Effects 0.000 claims abstract description 59
- 230000001105 regulatory effect Effects 0.000 claims abstract description 41
- 238000006243 chemical reaction Methods 0.000 claims abstract description 37
- 238000001514 detection method Methods 0.000 claims abstract description 20
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 4
- 239000004065 semiconductor Substances 0.000 claims description 4
- 229910052697 platinum Inorganic materials 0.000 claims description 2
- 238000001228 spectrum Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims 2
- 238000000034 method Methods 0.000 abstract description 15
- 230000003287 optical effect Effects 0.000 abstract description 12
- 230000000087 stabilizing effect Effects 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 description 37
- 230000003595 spectral effect Effects 0.000 description 20
- 238000001816 cooling Methods 0.000 description 19
- 239000002096 quantum dot Substances 0.000 description 19
- 238000010438 heat treatment Methods 0.000 description 18
- 238000012546 transfer Methods 0.000 description 16
- 238000005286 illumination Methods 0.000 description 13
- 239000000975 dye Substances 0.000 description 11
- 239000011159 matrix material Substances 0.000 description 7
- 230000037361 pathway Effects 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 230000005855 radiation Effects 0.000 description 5
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 239000010408 film Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 239000007844 bleaching agent Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 239000000806 elastomer Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- -1 for example Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 150000007523 nucleic acids Chemical group 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 101000863856 Homo sapiens Shiftless antiviral inhibitor of ribosomal frameshifting protein Proteins 0.000 description 1
- 230000005679 Peltier effect Effects 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- AMGQUBHHOARCQH-UHFFFAOYSA-N indium;oxotin Chemical compound [In].[Sn]=O AMGQUBHHOARCQH-UHFFFAOYSA-N 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002071 nanotube Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 229960001296 zinc oxide Drugs 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21K—NON-ELECTRIC LIGHT SOURCES USING LUMINESCENCE; LIGHT SOURCES USING ELECTROCHEMILUMINESCENCE; LIGHT SOURCES USING CHARGES OF COMBUSTIBLE MATERIAL; LIGHT SOURCES USING SEMICONDUCTOR DEVICES AS LIGHT-GENERATING ELEMENTS; LIGHT SOURCES NOT OTHERWISE PROVIDED FOR
- F21K9/00—Light sources using semiconductor devices as light-generating elements, e.g. using light-emitting diodes [LED] or lasers
- F21K9/60—Optical arrangements integrated in the light source, e.g. for improving the colour rendering index or the light extraction
- F21K9/64—Optical arrangements integrated in the light source, e.g. for improving the colour rendering index or the light extraction using wavelength conversion means distinct or spaced from the light-generating element, e.g. a remote phosphor layer
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V29/00—Protecting lighting devices from thermal damage; Cooling or heating arrangements specially adapted for lighting devices or systems
- F21V29/50—Cooling arrangements
- F21V29/54—Cooling arrangements using thermoelectric means, e.g. Peltier elements
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V29/00—Protecting lighting devices from thermal damage; Cooling or heating arrangements specially adapted for lighting devices or systems
- F21V29/50—Cooling arrangements
- F21V29/60—Cooling arrangements characterised by the use of a forced flow of gas, e.g. air
- F21V29/67—Cooling arrangements characterised by the use of a forced flow of gas, e.g. air characterised by the arrangement of fans
- F21V29/677—Cooling arrangements characterised by the use of a forced flow of gas, e.g. air characterised by the arrangement of fans the fans being used for discharging
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V29/00—Protecting lighting devices from thermal damage; Cooling or heating arrangements specially adapted for lighting devices or systems
- F21V29/50—Cooling arrangements
- F21V29/70—Cooling arrangements characterised by passive heat-dissipating elements, e.g. heat-sinks
- F21V29/74—Cooling arrangements characterised by passive heat-dissipating elements, e.g. heat-sinks with fins or blades
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0286—Constructional arrangements for compensating for fluctuations caused by temperature, humidity or pressure, or using cooling or temperature stabilization of parts of the device; Controlling the atmosphere inside a spectrometer, e.g. vacuum
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/1919—Control of temperature characterised by the use of electric means characterised by the type of controller
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21Y—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES F21K, F21L, F21S and F21V, RELATING TO THE FORM OR THE KIND OF THE LIGHT SOURCES OR OF THE COLOUR OF THE LIGHT EMITTED
- F21Y2115/00—Light-generating elements of semiconductor light sources
- F21Y2115/10—Light-emitting diodes [LED]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/063—Illuminating optical parts
- G01N2201/0638—Refractive parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/121—Correction signals
- G01N2201/1211—Correction signals for temperature
Landscapes
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Automation & Control Theory (AREA)
- Optics & Photonics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Fastening Of Light Sources Or Lamp Holders (AREA)
- Arrangement Of Elements, Cooling, Sealing, Or The Like Of Lighting Devices (AREA)
- Circuit Arrangement For Electric Light Sources In General (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A system is provided that includes a light-emitting diode (LED); a temperature sensor in thermal contact with the LED and capable of measuring an operating temperature and generating an operating temperature signal; and a temperature regulating system capable of receiving the operating temperature signal and regulating the operating temperature based on the operating temperature signal. A method for stabilizing the temperature of an LED is provided. A method is provided that includes providing a system comprising an LED, a reaction region, and a sample in the reaction region; generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the- operating temperature of the light emitting diode; and adjusting the detection data of an excitation beam characteristic shift related to the operating temperature, when the LED is operated at the operating temperature to generate the excitation beams.
Description
- The present application is a continuation-in-part of co-pending U.S. patent application Ser. No. 10/440,719, filed May 19, 2003, which in turn is a continuation-in-part of co-pending U.S. patent application Ser. No. 10/216,620, filed Aug. 9, 2002, which in turn is a continuation of co-pending U.S. patent application Ser. No. 09/700,536, filed Nov. 29, 2001, which claims priority to PCT/US99/11088, filed May 17, 1999, which published as publication number WO 99/60381 on Nov. 29, 1999, all of which are incorporated herein in their entireties by reference. Cross-reference is made to co-pending U.S. patent application Ser. No. 10/440,920 entitled “Optical Instrument Including Excitation Source” to Boege et al. (Attorney Docket No. 5010-027-01), co-pending U.S. patent application Ser. No. 10/440,852 entitled “Apparatus And Method For Differentiating Multiple Fluorescence Signals By Excitation Wavelength” to King et al. (Attorney Docket No. 5010-047-01), both filed on May 19, 2003, and to U.S. patent application Ser. No. 10/735,339, filed Dec. 12, 2003, all of which are incorporated herein in their entireties by reference.
- The present teachings relate to an optical instrument using excitation beams generated by a light-emitting diode.
- Light-Emitting Diodes (LEDs) can be used as an excitation source for optical detection, for example, in fluorescent measurement. There is a need for providing an LED excitation beam source that does not exhibit beam intensity changes and/or spectral shift. A device compatible with nucleotide amplification reactions, detecting such reactions, and capable of processing a relatively large number of amplification reactions is desirable.
- According to various embodiments, a system is provided that includes one or more light-emitting diode (LED), a temperature sensor, and a temperature regulator. The temperature sensor can be in thermal contact with the LED, can be capable of measuring an operating temperature, and can be capable of generating an operating temperature signal. The temperature regulator can be capable of receiving an operating temperature signal of the LED and regulating the operating temperature based on the operating temperature signal. Herein, it is to be understood that by LED what is meant is at least one LED, and that a group or array of LED's can be included in an “LED” as described herein.
- According to various embodiments, a method for illuminating a reaction region with excitation beams is provided. The method can include providing a system that includes an LED and a reaction region. The method can include generating excitation beams with the LED; directing the excitation beams toward the reaction region; measuring an operating temperature of the LED; and regulating the operating temperature by transferring heat away from and/or into the LED, based on the measured operating temperature. The reaction region can include a sample retained therein.
- According to various embodiments, a method for illuminating a reaction region with excitation beams is provided. The method can include providing a system that includes an LED and a reaction region. The method can include generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the operating temperature of the light emitting diode; and adjusting the detection data based on the operating temperature. The adjustment can be made, for example, by shifting the detection data. The shifting of the detection data can include, for example, a shift in intensity, spectra, or both.
- Additional features and advantages of various embodiments will be set forth in part in the description that follows, and in part will be apparent from the description, or can be learned by practice of various embodiments. Other advantages of the various embodiments will be realized and attained by means of the elements and combinations exemplified in the application.
- Various embodiments of the present teachings are exemplified in the accompanying drawings. The teachings are not limited to the embodiments depicted in the drawings, and include equivalent structures and methods as set forth in the following description and as would be known to those of ordinary skill in the art in view of the present teachings. In the drawings:
-
FIG. 1 is a side view in partial cross-section of a system including a heater providing temperature stabilization for an LED array according to various embodiments; -
FIG. 2 is a view in partial side cross-section of a system including a thermoelectric device providing temperature stabilization for an LED array according to various embodiments; -
FIG. 3 a is a side view in partial side cross-section of a system including a fan and cooling fins providing temperature stabilization for an LED array according to various embodiments; -
FIG. 3 b is a top plan view of a capillary sample holder according to various embodiments; -
FIG. 4 is a top view in partial cross-section of a system including a fan and heating element providing temperature stabilization for an LED according to various embodiments; and -
FIG. 5 is a side view in a partial cross-section of a system providing a strong thermal conductive path according to various embodiments. - It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are intended to provide a further explanation of the various embodiments of the present teachings.
- According to various embodiments, a system is provided that includes one or more light-emitting diode (LED), a temperature sensor in thermal contact with the LED, and a temperature regulator. The temperature sensor can be capable of measuring an operating temperature and generating a signal. The signal can include an operating temperature signal. The signal can be a digital signal. The digital signal can be indicative of whether the temperature being sensed is above or below a set point. The temperature sensor can generate a signal without thermal contact with the LED. The temperature sensor does not have to directly generate an operating temperature signal but rather can simply indicate whether a temperature is above or below a set point. The temperature regulator can be capable of receiving the operating temperature signal and regulating the operating temperature based on the operating temperature signal.
- According to various embodiments, the system can include a heat-transfer device and a control unit capable of controlling the heat-transfer device. The heat-transfer device can include a fan capable of forming an air current in thermal contact with the LED. The heat-transfer device can include a cooling fin in thermal contact with the LED. The heat-transfer device can include a heater in thermal contact with the LED. The heat-transfer device can include a thermoelectric device in thermal contact with the LED. The thermoelectric device can be connected to a reversible-DC-power supply. According to various embodiments, the temperature regulator can include a temperature system that can be capable of increasing and/or decreasing the operating temperature of the LED.
- According to various embodiments, the temperature regulator can comprise a system adapted to control excitation of one or more fluorescent dyes. The temperature regulator can be adapted such that it is capable of maintaining the operating temperature within an operating temperature range including a minimum temperature and a maximum temperature separated by, for example, about 15° C., about 5° C., about 1° C., or about 0.5° C. The operating temperature range can also be specified as a nominal temperature and an acceptable deviation value range.
- According to various embodiments, the temperature regulator can be a temperature regulating system that can include a user input device that is capable of being programmed to maintain an operating temperature range including a minimum temperature and a maximum temperature. The system can include a display capable of displaying the operating temperature signal.
- According to various embodiments, the system can include an error signaling device capable of signaling an alarm when the operating temperature is greater than a maximum temperature. The error signaling device can signal an alarm when the operating temperature is less than a minimum temperature.
- According to various embodiments, the system can include a substrate in contact with the LED. The substrate can include, for example, a Printed-Circuit Board (PCB). According to various embodiments, the reaction region can include a sample retained therein.
- The sample can include one or more nucleotide. The reaction region can include reagents necessary to perform a nucleic acid sequence amplification reaction. The sample can include fluorescent dyes, labels, or markers. The system can include a detector capable of detecting an optical property of the reaction region.
- According to various embodiments, the temperature sensor can include a thermister, a thermocouple, a resistance temperature detector (RTD), a bandgap semiconductor temperature sensor, a non-contact temperature sensor, a bandgap semiconductor resistive temperature detector, a platinum resistive temperature detector, a bi-metallic temperature detector, a combination thereof, or the like.
-
FIG. 1 is side cross-sectional view of asystem 100, according to various embodiments, including anLED array 110 that includes a plurality ofLEDs 111. The system can also include afocal lens 106. Thefocal lens 106 can focus excitation beams emitted by theLED array 110. TheLED array 110 can be in physical and/or thermal contact with asubstrate 112. TheLED array 110 can include one or more rows or patterns of individual LEDs. Thesubstrate 112 can be a PCB. Aheating device 116, for example, a resistive heating element, can be provided in thermal contact with theLED array 110. Theheating device 116 can be included in, on, or in and on thesubstrate 112. Thesystem 100 can include atemperature sensor 118 in thermal contact theLED array 110. The temperature sensor can be centrally located with respect to theLED array 110. Thetemperature sensor 118 can be included on thesubstrate 112. A temperature regulator ortemperature regulating system 122 can be provided that is capable of receiving a signal from thetemperature sensor 118. Thetemperature sensor 118 andtemperature regulating system 122 can be integrated and/or can be of a unitary construction. Thetemperature regulating system 122 can control theheating device 116. Thetemperature regulating system 122 can control afan 114. Thetemperature regulating system 122 can control thefan 114 and theheating device 116. For example, thetemperature regulating system 122 can be used to control theheating device 116 to reach or maintain a nominal operating temperature while thefan 114 prevents the operating temperature from getting too high. This optimization can be used, for example, if theLED array 111 is not continuously on. Thefan 114 can direct an air current over one ormore cooling fins 104. The coolingfins 104 can be in thermal contact with theLED array 110, with thesubstrate 112, or with both. Thetemperature regulating system 122 can send signals to and/or receive signals from thetemperature sensor 118, theheating device 116, and/or thefan 114. Thetemperature regulating system 122 can send and receivesignals using wires 120. Excitation beams can be emitted fromLED array 110 and directed to one ormore reaction region 108. Thereaction region 108 can include asample 107. The reaction region can be a microtiter tray. -
FIG. 2 is a side cross-sectional view of asystem 200, according to various embodiments, that includes a temperature stabilization device for anLED array 210, for example, by including a plurality ofLEDs 111. Afocal lens 206 can be included to focus excitation beams emitted from each of theindividual LEDs 211. TheLED array 210 can be in physical and/or thermal contact with asubstrate 212. Thesystem 200 can include atemperature sensor 218 in thermal contact with theLED array 210, thesubstrate 212, or both. Thetemperature sensor 218 can be included in or on thesubstrate 212. Atemperature regulating system 222 can receive a signal from thetemperature sensor 218. Thetemperature regulating system 222 can control athermoelectric device 214, for example, a Peltier device. Thethermoelectric device 214 can be in thermal contact with theLED array 210, withsubstrate 212, or with both. Thethermoelectric device 214 can transfer thermal energy from an ambient environment to theLED array 210. Thethermoelectric device 214 can transfer thermal energy to an ambient environment from theLED array 210. Thethermoelectric device 214 can include a temperature sensor. A plurality of coolingfins 204 can be in thermal contact with theLED array 210 and/or with thethermoelectric device 214. Thetemperature regulating system 222 can send signals to and/or receive signal from thetemperature sensor 218, and/or thethermoelectric device 214, for example, throughwires 220. Excitation beams can be emitted fromLED array 210 and can be directed to a plurality ofreaction regions 208, for example, held in athermal cycling block 230. Thethermoelectric device 214 can be used to maintain a lower temperature than could be otherwise achieved under operating conditions. This can permit theLED array 210 to operate more efficiently, with a higher total flux output. Thethermoelectric device 214 can be used in a heating mode, for example, to reach or maintain a temperature when theLED array 210 is not on. Thethermoelectric device 214 can be used in a cooling mode when the duty cycle of theLED array 210 is high enough to require cooling. -
FIG. 3 a is a side cross-sectional view of asystem 300 according to various embodiments and capable of providing temperature stabilization for anLED array 310 including a plurality ofindividual LEDs 311. A focal or collimatinglens 306 can be included to focus excitation beams emitted from each of theindividual LEDs 311. The collimating lens can be a Fresnel lens. Abeam splitter 307 can be included to separate excitation beams from emission beams. Thebeam splitter 307 can be replaced by a filter or beam splitter as described, for example, in U.S. patent application Ser. No. 10/735,339, filed Dec. 12, 2003, which is incorporated herein in its entirety by reference. TheLED array 310 can be in contact with asubstrate 312. Thesystem 300 can include atemperature sensor 318 in thermal contact with theLED array 310. Thetemperature sensor 318 can be included in, on, or in and on thesubstrate 312. Atemperature regulating system 322 can receive a signal from thetemperature sensor 318. Thetemperature regulating system 322 can control afan 314. Thefan 314 can direct an air current over a plurality of coolingfins 304. The coolingfins 304 can be in physical and/or thermal contact with theLED array 310. Thetemperature regulating system 322 can communicate with thetemperature sensor 318, and/or the fan, throughwires 320. Excitation beams can be emitted fromLED array 310 and directed to a reaction region 308 formed or disposed in, on, or in and on asubstrate 309. The reaction regions can includecapillaries 330 of a capillary array. Thecapillaries 330 can each have a portion that passes through adetection zone 356. - According to various embodiments, the temperature control system can include a heater. The system can include a cooler. The system can include both a heater and a cooler. Cooling and heating rates can be augmented by using a plurality of heaters and/or coolers as desired. If a heater is provided, it can comprise a plurality of different types of heating devices. If a cooler is provided, it can comprise a plurality of different types of cooling devices.
-
FIG. 3 b is a top plan partial view of the array ofcapillaries 330 shown inFIG. 3 a, and thedetection zone 356. The capillaries can traverse thedetection zone 356, where excitation beams from the LED array 310 (FIG. 3 a) can be directed. For example, the excitation beams can be used for fluorescence detection of analytes in capillaries of a capillary electrophoresis device. Such can be the case in DNA sequencing and fragment length analysis applications. - An LED illumination system can provide consistent illumination, can be light in weight, and can require minimal cooling and/or heating. The LED can be a standard semi-conductor device, an organic LED, or an inorganic LED. Examples of organic LEDs are QDOT-based LEDs and a nanotube-based LEDs. The LED can be a stack of LED's such as a stack of organic LEDs or a stack of organic LED layers.
- According to various embodiments, LEDs producing several different excitation wavelengths can be used, either simultaneously or sequentially. The use of a plurality of different excitation wavelengths can improve the calibration matrix necessary to distinguish fluorescence emissions of various dyes.
- According to various embodiments, a system can comprise LEDs, photodiodes, operational amplifiers, and LED-current control circuits. The components of the system can change properties with operating temperature variations. A temperature regulating system can maintain these components at a constant temperature. The constant temperature can be elevated from an ambient temperature. The constant temperature can be lower than an ambient temperature. For example, the system components can be held at a constant temperature above an ambient temperature using a resistive heating element as a heat source under the control of the temperature regulating system. A strong or high thermal conductivity pathway can be used between the system components, for example, to the temperature sensor from a heat source and/or a heat sink.
- The temperature sensor can be used to measure directly, indirectly, or by calculation, the temperature of the system components. The temperature sensor can measure an operating temperature for various components of the system. The temperature sensor can provide feedback to a temperature regulating system. The temperature regulating system can monitor the amount of heating or cooling provided by a heat source or a heat sink to maintain the system components at a nominal temperature within an acceptable deviation value range.
- The temperature control characteristics of a temperature regulating system can be improved by enclosing the system components in a thermally isolated environment. For example, the system components and the temperature sensor, and/or the temperature regulating system, can be placed in an enclosure or housing. The enclosure can have openings for allowing illumination from the LEDs to illuminate a detection zone. Heat exchange pathways can be disposed in the enclosure to allow for thermal transfer between the system and an ambient environment. The heat exchange pathway can be a vent in the enclosure. A cooling fan can cool the thermally isolated environment provided by the enclosure. The heat exchange pathway can include, for example, a high conductivity thermal surface included in the enclosure and in thermal contact with a thermoelectric device. The system components can be separated from the enclosure using a thermal insulator to lower a heat exchange rate between the enclosure and the temperature control components. The temperature sensor can be in thermal contact with, in heat-transfer communication with, or otherwise thermally coupled to, the substrate. Known methods of heat transfer include, but are not limited to conduction, convection, and thermal radiation.
- According to various embodiments, a heat conductive adhesive or compliant pad can be used to attain good thermal conductivity between a heat sink or heat source, and other system components, for example, to maintain temperature stability in the system. A heat exchange pathway can be established for system components such as photodiodes and LEDs using a ground path to the same metal or layer plate, for example, in a PCB. The plate can be a metal, for example, aluminum, copper, or other electrically conductive metals. The system can thus maintain temperature stability and keep various system components at substantially the same temperature. The heat exchange pathway can exchange heat with the ground plate. Other temperature interface materials, for example, adhesive backed resistive elements, can be used to achieve good contact with the system components. A resistive heater can be disposed in or on a common substrate shared with other electrical circuits included in the system.
-
FIG. 4 is a top plan cross-sectional view of asystem 400. Ahousing 401, also known as a cave, an oven, or an enclosure, can include openings such as 403 and 407 as shown.LEDs 413, 415 can irradiate through respective openings (403) to illuminate one or more reaction regions (not shown). Theopening 407 can allow transmission or passing of emission beams from a reaction region to adetector 440. One ormore temperature sensor 418 can be disposed in or on ahousing substrate 412. Thesubstrate 412 can include aheating device 416. Thetemperature sensor 418 can be disposed on or in thehousing substrate 412.LEDs 413 and 415, anddetector 440, can be disposed on or in thehousing substrate 412. A temperature regulator ortemperature regulating system 422, capable of receiving a signal from thetemperature sensor 418, can be included, for example, in thehousing 412. Thetemperature regulating system 422 can control theheating device 416 and/or a coolingfan 414, as desired, for example, to maintain thesystem 400 within a desired or pre-set temperature range. Thehousing 401 can provide a relatively small, thermally isolated, volume to be temperature-regulated by thetemperature regulating system 422. Control circuits (not shown) necessary to utilize theLEDs 413, 415 and thedetector 440 can be housed within thehousing 401. Excitation beams can be emitted from theLEDs 413, 415 and directed toward one or more reaction regions. LED 413 can produce excitation beams of a different wavelength range thanLED 415, for example, LED 413 can produce blue light andLED 415 can produce green light. LED 413 can be operated simultaneously or sequentially withLED 415. -
FIG. 5 is a side cross-sectional view of a system 500 according to various embodiments. The system 500 can includephotodiode detectors substrate 574. Thesubstrate 574 can havecontrol circuits LED 513 mounted on aplate 568 having a thermal conductivity of about 0.1 w/cm·k or higher, for example, about 0.3 w/cm·k or higher or about 0.5 w/cm·k or higher. For example, theplate 568 can comprise, for example, aluminum, steel, stainless steel, another metal or alloy, a printed circuit board, or a combination thereof. Anelastomer pad 570 having a high thermal conductivity can be disposed between thesubstrate 574 and theplate 568. The substrate can be a multi-layer structure including a layer having a thermal conductivity of about 0.1 w/cm·k or higher. Theelastomer pad 570 can electrically isolate an electricresistive heater 518 from thesubstrate 574. Thephotodiode detectors substrate 574 using, for example, an adhesive 572. A temperature sensor 519 can be disposed in thermal contact with the system 500, for example, the temperature sensor 519 can be disposed in contact with theplate 568.Thermal insulation 576 can be disposed adjacent the second surface or backside 575 of thesubstrate 574 to thermally isolate the system 500 from an ambient environment. The system can maintain thecontrol circuits photodiode detectors LEDs - Various embodiments depicting configurations of LED's, reaction regions, and intervening devices that can be used to direct excitation beams from light sources, for example, LEDs, toward reaction regions, can be found, for example, in U.S. patent application Ser. No. 10/440,920, filed May 19, 2003, entitled “Optical Instrument Including Excitation Source” to Boege et al., U.S. patent application Ser. No. 10/440,719, filed May 19, 2003, entitled “Optical Instrument Including Excitation Source” to Boege et al., U.S. patent application Ser. No. 10/440,852, filed May 19, 2003, entitled “Apparatus And Method For Differentiating Multiple Fluorescence Signals By Excitation Wavelength” to King et al., U.S. patent application Ser. No. 10/735,339, filed Dec. 12, 2003, and International Publication No. PCT/US01/35079. All Patents, Patent Applications, and publications mentioned herein are incorporated herein in their entireties by reference.
- The LED or the LED array can include a plurality of LEDs mounted on a substrate. The LED can thermally contact a temperature regulating system. The temperature regulating system can control a heat-transfer device and/or a temperature sensor. The temperature regulating system can maintain the operating temperature of the LED such that the operating temperature does not change appreciably, by not more than 0.5° C., that is, does not fluctuate by more than 10° C. during operation, for example, by not more than 5° C., by not more than 1° C., by not more than 0.5° C., or by not more than 0.1° C. or less. The temperature regulating system can maintain the operating temperature of the LED such that the operating temperature does not exceed the bounds of a programmed temperature range. According to various embodiments, a temperature regulating system and a temperature sensor can be included in a single-unit or can be included in an integrated device, for example, a Maxim DS1620 device available from Maxim Integrated Products, Inc. of Sunnyvale, Calif.
- The temperature sensor and the LED do not necessarily have to be in physical contact. The temperature regulating system can adjust a monitored temperature of the LED to compensate for any thermal masses intervening between the LED and the temperature sensor and to thus derive, calculate, or estimate an operating temperature.
- According to various embodiments, the LED can be cooled to maintain life and illumination uniformity requirements of a system. According to various embodiments, a forced air cooling system or a thermoelectric device, for example, a Peltier device, can be used to cool the LED and to keep the LED from exceeding a maximum operating temperature.
- According to various embodiments, the temperature of the LED can be monitored, for example, with a temperature sensor, and thermal characteristics of a system and spectral characteristics of any LEDs embedded within the system, can be recorded. With understanding and reproducibility of the spectral coefficients of the LED as a function of an operating temperature, the effects of a spectral shift can be mitigated upon detection of optical properties of a sample. According to various embodiments, a dye matrix or detection data can be altered in accordance with the conditions under which the dye matrix or detection data was gathered or detected. Thermal effects on excitation beams emitted by LEDs, including spectral shifts and intensity changes, can thus be minimized or effectively eliminated. According to various embodiments, the temperature of an LED can be monitored and a computing apparatus can adjust the detection data to compensate for the spectral shifts and/or intensity changes of excitation beams emitted from the LED. The compensation for the shifting can be varied across wavelength ranges, for example, different compensations can be provided for different wavelengths of LEDs. A system can be provided that can include a data adjustment unit comprising a memory adapted to store at least two operating temperatures and at least one respective excitation beam characteristic shift for each operating temperature. A plurality of respective excitation beam characteristic shifts can be stored in the memory. The adjustment data can be in the form of a plurality of respective coefficients. Each coefficient can correspond to a respective LED of an LED array. An exemplary range of coefficients can be from about 0.4 nm/° C. to about 4.0 nm/° C., for example, based on deviation from a set or average operating temperature. The coefficients can include two or more nominal temperature coefficients corresponding to two or more LEDs. The coefficients can be determined or designated based on the position of a respective LED in an LED array.
- According to various embodiments, optical detection instruments utilizing LEDs can obtain very stable intensity or spectral characteristics by stabilizing an operating temperature of an LED. Illumination stability can be important to minimize the signal noise in the system. Illumination stability can improve the sensitivity of the instrument to detect low concentration dyes. Spectral stability can be used to maintain values for the deconvolution matrix associated with a set of dyes to prevent quantification errors. Similarly, variations in intensity resulting from temperature changes can be different for different wavelengths of LEDs, resulting in apparent spectral instability.
- According to various embodiments, illumination stability can be improved by allowing the illumination source to warm-up. According to various embodiments, shutters can block excitation beams from reaching a sample to prevent bleach out. According to various embodiments, shutters can block excitation beams from reaching a sample to prevent bleach out during illumination source warm-up. The illumination source can be brought to a desired operating temperature range prior to enabling or turning on the illumination source, using a heater and/or a cooler. Regulating the temperature of the illumination source prior to enabling the illumination source can prevent the need for a shutter and/or can reduce the warm-up time period. According to various embodiments, samples can be subjected to a reaction or a series of reactions, for example, temperature cycled in a nucleic acid sequence amplification or in a sequencing process. According to various embodiments, the shutter can be unblocked in co-ordination with the reaction or the series of reactions, to detect and collect data at an appropriate time, for example, during a fluorescence detection reading of the sample.
- According to various embodiments, sensitivity of the instrument to detect low concentration dyes can be used. An LED can shift, for example, 5% over a 15° C. ambient temperature range maintained by some optical instrumentation. According to various embodiments, a spectral shift of an LED can vary depending on a center wavelength of the LED, for example, blue LEDs can shift less than red LEDs. The spectral shift can be from about 0.04 nm/° C. to about 0.4 nm/° C. The spectral shift can be different for different temperatures. The spectral shift can be calculated. The spectral shift can be obtained from a look-up table. The table can be sorted by temperature, for example. The table can be provided in a long-term storage of a computer system, for example. According to various embodiments, some optical instrumentation can be sensitive to a dye shift of about 1 nm or less. According to various embodiments, laboratory instrumentation utilizing a relatively more robust dye matrix can be less susceptible to the spectral shift of an LED than a system with a relatively less robust dye matrix. The AB 7500 system available from Applied Biosystems of Foster City, Calif., can have a very good dye matrix and can have little susceptibility to spectral shift for at least most dyes.
- According to various embodiments, the LED radiation source can contain one Light Emitting Diode (LED) or an array of individual LEDs. According to various embodiments, each LED can be a high power LED that can emit greater than or equal to about 1 mW of excitation energy. In various embodiments, a high power LED can be used that can emit at least about 5 mW of excitation energy. In various embodiments wherein the LED or array of LEDs can emit, for example, at least about 50 mW of excitation energy, a cooling device such as, but not limited to, a heat sink or fan can be used with the LED. An array of high-powered LEDs can be used that draws, for example, about 10 watts of energy or less, about five watts of energy or less, or about 3 watts of energy or less. Exemplary LED array sources are available, for example, from Stocker Yale (Salem, N.H.) under the trade name LED AREALIGHTS, and from Lumileds Lighting, LLC (San Jose, Calif.) under the trade name Luxeon Star. According to various embodiments, LED light sources can use about 1 microwatt (μW) of power or more, for example, about 5 mW, about 25 mW, about 50 mW, about 1 W, about 5 W, about 50 W, or about 100 W or more, each individually or collectively when used in an array.
- According to various embodiments, the light source can include a combination of two, three, or more LEDs, laser diodes, and the like, such as, for example, an LED that can emit radiation at about 475 nm, an LED that can emit radiation at about 539 nm, and an LED that can emit radiation at about 593 nm. The LED can be, for example, an Organic Light Emitting Diode (OLED) an inorganic Light Emitted Diode, that can be polymer-based or small-molecule-based (organic or inorganic), an edge emitting diode (ELED), a Thin Film Electroluminescent Device (TFELD), or a Quantum dot based inorganic “organic LED.” The LED can include a phosphorescent OLED (PHOLED). Super bright LEDs can be used and can be arranged in a light array. According to various embodiments, separate LEDs or a packaged set of LEDs can be used in an array. Spectral emissions of the light sources can be effected by an operating temperature of the light source. Other suitable light sources will be apparent to practitioners in the art given the teachings herein. OLEDs can be used as an array while being designed as a single part. As used herein, the terms “excitation source,” “irradiation source,” and “light source” are used interchangeably.
- According to various embodiments, excitation beams emitted from the light source can diverge from the light source at an angle of divergence. The angle of divergence can be, for example, from about 5° to about 75° or more. The angle of divergence can be substantially wide, for example, greater than 45°, yet can be efficiently focused by use of a lens, such as the focusing lens 106 (
FIG. 1 ), 206 (FIG. 2 ), and 306 (FIG. 3 ). The lens can be a collimating lens, for example, a Fresnel lens. - According to various embodiments, a quantum dot can be used as a source for luminescence and as a fluorescent marker. Quantum dots can be used for both. The quantum dot based LED can be tuned to emit light in a tighter emission bandpass, for example, an emission bandpass including a full-width of half-max of about 10 nm or less, about 20 nm or less, or about 50 nm or less. The quantum dot based LED can increase the efficiency of the fluorescent system. The efficiency of a quantum dot based LED can theoretically be higher than that of conventional LEDs, potentially over 90% when sandwiched directly between two conductive films with each film directly touching each quantum dot as opposed to the present 20% efficiency for standard LEDs. Quantum dot based LEDs can be made utilizing a slurry of quantum dots, where current flows through an average of several quantum dots before being emitted as a photon. This conduction through several quantum dots can cause resistive losses in efficiency. Quantum dots can provide many more colors than conventional LEDs.
- A Quantum dot based LED can emit light in an emission band that is narrower than an emission band of a normal LED, for example, about 50% narrower or about 25% narrower. The emission band of the quantum dots can be a function of the size distribution of the quantum dots, and thus can theoretically be extremely narrow. The Quantum dot based LED can also emit light at an electrical energy conversion efficiency of about, 90% or more, for example, approaching 100%. OLED films, including Quantum dot based LEDs, can be applied to a thermal block, used for heating and cooling samples, in a fluorescence system without interfering with the operation of the thermal block.
- According to various embodiments, an OLED can be used and/or produced on a flexible substrate, on an optically clear substrate, on a substrate of an unusual shape, or on a combination thereof. Multiple OLEDs can be combined on a substrate, wherein the multiple OLEDs can emit light at different wavelengths. Multiple OLEDs on a single substrate or multiple adjacent substrates can form an interlaced or a non-interlaced pattern of light of various wavelengths. The pattern can correspond to, for example, a sample reservoir arrangement or array. One or more OLEDs can form a shape surrounding, for example, a sample reservoir, a series of sample reservoirs, an array of a plurality of sample reservoirs, or a sample flow path. The sample flow path can be, for example, a channel, a capillary, or a micro-capillary. One or more OLEDs can be formed to follow the sample flow path. One or more OLEDs can be formed in the shape of a substrate or a portion of a substrate. For example, the OLED can be curved, circular, oval, rectangular, square, triangular, annular, or any other geometrically regular shape. The OLED can be formed as an irregular geometric shape. The OLED can illuminate one or more sample reservoirs, for example, an OLED can illuminate one, two, three, four, or more sample reservoirs simultaneously, or in sequence. The OLED can be designed, for example, to illuminate all the wells of a corresponding multi-well array.
- According to various embodiments, an OLED can be used and can be formed from one or more stable, organic materials. The OLED can include one or more carbon-based thin films and the OLED can be capable of emitting light of various colors when a voltage is applied across the one or more carbon-based thin films. Various LEDs can use different films, for example, quantum dot based LEDs can use Indium tin oxide.
- According to various embodiments, an operating temperature of an LED can be controlled with a Peltier-effect thermoelectric device, a heat pump, an electrical resistance heating element (Joule heater), fluid-flow through channels in a metal block, reservoirs of fluid at different temperatures, tempered air impingement, a combination thereof, or the like. According to various embodiments, the thermal device can include a fan to direct air-flow over cooling fins, or a cold bar to assist in a heat transfer between an LED and another thermal mass, such as air. According to various embodiments, the thermal conductivity of the LED and/or a platform supporting the LED can be greater than that of a surrounding ambient environment, for example, the surrounding air.
- According to various embodiments, a thermoelectric device can be used as a heat-transfer device, for example, an XLT module available from Marlow Industries, Inc. of Dallas, Tex. Controls for the thermoelectric device can include an adjustable-bipolar DC output current power supply. The power supply can provide programmable PID control/ramp to set point control, deviation alarms, and automatic and manual operating modes. In reactions, for example, real-time monitoring of Polymerase Chain Reaction (PCR) reactions, thermoelectric devices can both heat and cool, as desired, the LED by using a bi-directional power supply under programmable and/or logic control. The programmable and logic control can be provided by using a general purpose computer, or custom built hardware, for example, a field programmable gate array (FPGA). Thermoelectric devices can be specifically designed to withstand the continuous temperature excursions required in PCR use.
- According to various embodiments, a heat-transfer device can include a vapor-cycle device, for example, a Freon-based vapor compression or absorption refrigerator. In such units, thermal energy can be extracted from a region, thereby reducing its temperature, then rejected to a “heat sink” region of higher temperature. Vapor-cycle devices can include moving mechanical parts and can include a working fluid, while thermoelectric elements can be totally solid state.
- According to various embodiments, a thermal interface material (TIM) can provide a good thermal contact between two surfaces, for example, between an LED support and a substrate, and/or between an LED housing and a thermoelectric device. The TIM can include silicone-based greases, elastomeric pads, thermally conductive tapes, thermally conductive adhesives, or a combination thereof. Zinc-oxide silicone can be used as a TIM. According to various embodiments, Gap-Pad products, for example, GAP PAD VO ULTRA SOFT materials or SIL-PAD, materials available from Berquist Company of Chanhassen, Minn., can be used a thermal interface materials. A TIM is described in U.S. Pat. No. 5,679,457 to Bergerson, which is incorporated herein in its entirety. According to various embodiments, a TIM can be disposed between a heat-transfer device and an LED.
- According to various embodiments, a method can be provided for maintaining emission intensity and spectral stability of an LED. The method can comprise: providing a system comprising an LED; generating excitation beams with the LED; measuring an operating temperature of the LED; and regulating the operating temperature by at least one of transferring heat from the LED and transferring heat to the LED, based on the operating temperature, to maintain emission intensity and spectral stability of the LED. The regulating can comprise retrieving from a memory source adjustment data corresponding to a desired operating temperature or temperature range at which emission intensity and spectral stability of the LED are maintained.
- Other embodiments will be apparent to those skilled in the art from consideration of the present specification and practice of various embodiments disclosed herein. It is intended that the present specification and examples be considered as exemplary only.
Claims (13)
1-20. (canceled)
21. A system comprising:
a reaction region;
a light-emitting diode (LED) capable of generating and directing excitation beams toward the reaction region;
a temperature sensor in thermal contact with the LED and capable of measuring an operating temperature of the LED and generating an operating temperature signal;
a detector adapted to detect emission signals from the reaction region and capable of generating detection data; and
a data adjustment unit capable of receiving the operating temperature signal and the detection data, and adapted to adjust the detection data for an excitation beam characteristic shift related to the operating temperature, to form shifted detection data.
22. The system of claim 21 , wherein the data adjustment unit comprises a memory adapted to store at least two operating temperatures and at least one respective excitation beam characteristic shift for each operating temperature.
23. The system of claim 21 , wherein the temperature sensor comprises at least one of a thermistor, a thermocouple, a bandgap semiconductor resistive temperature detector, a platinum resistive temperature detector, or a bi-metallic temperature detector.
24. The system of claim 21 , wherein the LED comprises a plurality of light-emitting diodes that are capable of emitting different respective wavelength ranges.
25. The system of claim 24 , wherein the data adjustment unit comprises a memory adapted to store at least two operating temperatures, and at least one respective excitation beam characteristic shift for each operating temperature, and wherein the at least one respective excitation beam characteristic shift comprises at least one respective excitation beam characteristic shift for each different wavelength range.
26. The system of claim 24 , wherein the plurality of LEDs comprises at least one blue LED and at least one green LED.
27. The system of claim 21 , further comprising at least one separation region, and wherein the LED is capable of generating and directing excitation beams toward the at least one separation region.
28. The system of claim 21 , wherein the LED comprises a plurality of LEDs disposed adjacent one another and each LED has a respective operating temperature and a respective excitation beam characteristic shift.
29. The system of claim 21 , wherein the LED comprises a plurality of LEDs stacked along a path for directing excitation beams towards the reaction region, and each LED has a respective operating temperature and a respective excitation beam characteristic shift.
30. The system of claim 21 , further comprising a temperature regulating system capable of receiving the operating temperature signal and regulating the operating temperature based on the operating temperature signal.
31. The system of claim 21 , wherein the excitation beam characteristic comprises a spectrum of the generated excitation beams.
32. The system of claim 21 , wherein the excitation beam characteristic comprises an intensity of the generated excitation beams.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/895,848 US20110159549A1 (en) | 1999-05-17 | 2010-09-30 | Temperature control for light-emitting diode stabilization |
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1999/011088 WO1999060381A1 (en) | 1998-05-16 | 1999-05-17 | Instrument for monitoring polymerase chain reaction of dna |
US09/700,536 US6818437B1 (en) | 1998-05-16 | 1999-05-17 | Instrument for monitoring polymerase chain reaction of DNA |
US10/216,620 US7008789B2 (en) | 1998-05-16 | 2002-08-09 | Instrument for monitoring polymerase chain reaction of DNA |
US10/440,719 US7387891B2 (en) | 1999-05-17 | 2003-05-19 | Optical instrument including excitation source |
US10/981,440 US20050279949A1 (en) | 1999-05-17 | 2004-11-04 | Temperature control for light-emitting diode stabilization |
US11/644,410 US20070105212A1 (en) | 1999-05-17 | 2006-12-22 | Temperature control for light-emitting diode stabilization |
US12/895,848 US20110159549A1 (en) | 1999-05-17 | 2010-09-30 | Temperature control for light-emitting diode stabilization |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/644,410 Continuation US20070105212A1 (en) | 1999-05-17 | 2006-12-22 | Temperature control for light-emitting diode stabilization |
Publications (1)
Publication Number | Publication Date |
---|---|
US20110159549A1 true US20110159549A1 (en) | 2011-06-30 |
Family
ID=35929963
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/981,440 Abandoned US20050279949A1 (en) | 1999-05-17 | 2004-11-04 | Temperature control for light-emitting diode stabilization |
US11/644,410 Abandoned US20070105212A1 (en) | 1999-05-17 | 2006-12-22 | Temperature control for light-emitting diode stabilization |
US12/895,848 Abandoned US20110159549A1 (en) | 1999-05-17 | 2010-09-30 | Temperature control for light-emitting diode stabilization |
US13/619,679 Expired - Fee Related US9285318B2 (en) | 1999-05-17 | 2012-09-14 | Optical instrument including excitation source |
US15/065,834 Abandoned US20160305881A1 (en) | 1999-05-17 | 2016-03-09 | Optical instrument including excitation source |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/981,440 Abandoned US20050279949A1 (en) | 1999-05-17 | 2004-11-04 | Temperature control for light-emitting diode stabilization |
US11/644,410 Abandoned US20070105212A1 (en) | 1999-05-17 | 2006-12-22 | Temperature control for light-emitting diode stabilization |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/619,679 Expired - Fee Related US9285318B2 (en) | 1999-05-17 | 2012-09-14 | Optical instrument including excitation source |
US15/065,834 Abandoned US20160305881A1 (en) | 1999-05-17 | 2016-03-09 | Optical instrument including excitation source |
Country Status (4)
Country | Link |
---|---|
US (5) | US20050279949A1 (en) |
EP (3) | EP1890182A2 (en) |
JP (4) | JP2008519266A (en) |
WO (1) | WO2006052682A2 (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050279949A1 (en) * | 1999-05-17 | 2005-12-22 | Applera Corporation | Temperature control for light-emitting diode stabilization |
US8900282B2 (en) | 2005-02-17 | 2014-12-02 | Biolux Research Ltd. | Light therapy apparatus and methods |
CN105351899A (en) * | 2015-09-23 | 2016-02-24 | 华南理工大学 | LED heat-dissipating device adopting semiconductor refrigerating plate and phase change materials |
CN105451635A (en) * | 2013-08-23 | 2016-03-30 | 奥林巴斯株式会社 | Light source device and endoscope device |
CN105987314A (en) * | 2015-05-16 | 2016-10-05 | 深圳市金达照明有限公司 | Lamp capable of automatically adjusting light source temperature |
US9730780B2 (en) | 2013-10-22 | 2017-08-15 | Biolux Research Ltd. | Intra-oral light-therapy apparatuses and methods for their use |
CN111788867A (en) * | 2018-06-15 | 2020-10-16 | 伊诺瓦半导体有限责任公司 | Method and system device for setting constant wavelength |
Families Citing this family (126)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7498164B2 (en) | 1998-05-16 | 2009-03-03 | Applied Biosystems, Llc | Instrument for monitoring nucleic acid sequence amplification reaction |
EP3093649B1 (en) | 1998-05-16 | 2019-05-08 | Life Technologies Corporation | A combination of a reaction apparatus and an optical instrument monitoring dna polymerase chain reactions |
BR0312430A (en) | 2002-06-19 | 2005-04-26 | Palomar Medical Tech Inc | Method and apparatus for treating skin and subcutaneous conditions |
TWI257465B (en) * | 2004-10-11 | 2006-07-01 | Neobulb Technologies Inc | Lighting device with high heat dissipation efficiency |
EP1880585A1 (en) * | 2005-03-03 | 2008-01-23 | Tir Systems Ltd. | Method and apparatus for controlling thermal stress in lighting devices |
CN100468795C (en) * | 2005-06-03 | 2009-03-11 | 新灯源科技有限公司 | Semiconductor illuminator integrated heat conducting/radiating moudule |
KR100643764B1 (en) * | 2005-03-09 | 2006-11-10 | 삼성전자주식회사 | Image projection apparatus for adjusting white balance by referring to temperature of LED and method thereof |
KR100643774B1 (en) * | 2005-03-09 | 2006-11-10 | 삼성전자주식회사 | Image projection apparatus for adjusting white balance by referring to temperature and light emitting level of LED and method thereof |
CN1840958B (en) * | 2005-03-28 | 2012-07-04 | 新灯源科技有限公司 | System-in-package high-power high-efficiency diode bulb |
JP5177554B2 (en) * | 2005-03-31 | 2013-04-03 | 新灯源科技有限公司 | Lighting equipment using high power LEDs with high efficiency heat dissipation |
US7856985B2 (en) | 2005-04-22 | 2010-12-28 | Cynosure, Inc. | Method of treatment body tissue using a non-uniform laser beam |
US7446288B2 (en) * | 2005-05-06 | 2008-11-04 | Applied Biosystems Inc. | Device including inductively heatable fluid retainment region, and method |
CN1869504B (en) * | 2005-05-25 | 2010-04-07 | 新灯源科技有限公司 | LED cluster bulb |
US8718437B2 (en) | 2006-03-07 | 2014-05-06 | Qd Vision, Inc. | Compositions, optical component, system including an optical component, devices, and other products |
WO2007103310A2 (en) | 2006-03-07 | 2007-09-13 | Qd Vision, Inc. | An article including semiconductor nanocrystals |
US20070194247A1 (en) * | 2005-08-31 | 2007-08-23 | Stratagene California | Compact optical module for fluorescence excitation and detection |
US7821123B2 (en) * | 2005-09-13 | 2010-10-26 | Delphi Technologies, Inc. | LED array cooling system |
TWI391600B (en) * | 2005-09-27 | 2013-04-01 | Koninkl Philips Electronics Nv | Led lighting fixtures |
US8327657B2 (en) * | 2005-10-27 | 2012-12-11 | Lg Electronics Inc. | Refrigerator |
US9951438B2 (en) | 2006-03-07 | 2018-04-24 | Samsung Electronics Co., Ltd. | Compositions, optical component, system including an optical component, devices, and other products |
US9874674B2 (en) | 2006-03-07 | 2018-01-23 | Samsung Electronics Co., Ltd. | Compositions, optical component, system including an optical component, devices, and other products |
WO2007116675A1 (en) * | 2006-03-28 | 2007-10-18 | Terumo Kabushiki Kaisha | Body fluid components measuring device |
DE102006022351A1 (en) * | 2006-05-12 | 2007-11-15 | Patent-Treuhand-Gesellschaft für elektrische Glühlampen mbH | Device for room temperature regulation and room lighting |
US8232091B2 (en) | 2006-05-17 | 2012-07-31 | California Institute Of Technology | Thermal cycling system |
WO2007149696A1 (en) | 2006-06-23 | 2007-12-27 | Applera Corporation | Systems and methods for cooling in biological analysis instruments |
US20080002407A1 (en) * | 2006-06-28 | 2008-01-03 | Chen Jan J | Light emitting module for automatically adjusting lighting power and a method thereof |
DE102006036171B4 (en) * | 2006-07-28 | 2008-10-09 | Analytik Jena Ag | Arrangement and method for multichannel fluorescence measurement in PCR samples |
US7759882B2 (en) * | 2006-07-31 | 2010-07-20 | Microsemi Corp.—Analog Mixed Signal Group Ltd. | Color control for scanning backlight |
US7586957B2 (en) | 2006-08-02 | 2009-09-08 | Cynosure, Inc | Picosecond laser apparatus and methods for its operation and use |
KR20090063258A (en) * | 2006-09-14 | 2009-06-17 | 코닌클리즈케 필립스 일렉트로닉스 엔.브이. | Lighting assembly and method for providing cooling of a light source |
DE102006056596A1 (en) * | 2006-11-30 | 2008-06-05 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Fluorescence signal detecting device for use during investigation of fluorescent sample, has beam splitting device separating excitation and fluorescence signals, and control device providing excitation and receiving control signals |
US8836212B2 (en) | 2007-01-11 | 2014-09-16 | Qd Vision, Inc. | Light emissive printed article printed with quantum dot ink |
US8328387B2 (en) | 2007-01-17 | 2012-12-11 | Osram Gmbh | LED module |
US7733488B1 (en) * | 2007-01-26 | 2010-06-08 | Revolution Optics, Llc | Compact multi-wavelength optical reader and method of acquiring optical data on clustered assay samples using differing-wavelength light sources |
WO2008121761A1 (en) * | 2007-03-30 | 2008-10-09 | Fujifilm Corporation | Method of controlling temperature |
WO2008131584A1 (en) * | 2007-04-27 | 2008-11-06 | Jenshyan Chen | Light emitting diode lighting device |
JP5773646B2 (en) | 2007-06-25 | 2015-09-02 | キユーデイー・ビジヨン・インコーポレーテツド | Compositions and methods comprising depositing nanomaterials |
WO2009000106A1 (en) * | 2007-06-25 | 2008-12-31 | Jenshyan Chen | Led lighting device |
WO2009014707A2 (en) | 2007-07-23 | 2009-01-29 | Qd Vision, Inc. | Quantum dot light enhancement substrate and lighting device including same |
US8128249B2 (en) | 2007-08-28 | 2012-03-06 | Qd Vision, Inc. | Apparatus for selectively backlighting a material |
CN101498435A (en) * | 2008-01-30 | 2009-08-05 | 富士迈半导体精密工业(上海)有限公司 | Luminous diode lighting device and method for reducing heating power thereof |
DE102008001322A1 (en) * | 2008-04-22 | 2009-10-29 | Linos Photonics Gmbh & Co. Kg | Sample array analysis system for use in e.g. pharma research, has detector detecting luminescence radiation emitted by samples, and light conductor array arranged in front of sample plate for conducting light on samples |
WO2009137053A1 (en) | 2008-05-06 | 2009-11-12 | Qd Vision, Inc. | Optical components, systems including an optical component, and devices |
US9207385B2 (en) | 2008-05-06 | 2015-12-08 | Qd Vision, Inc. | Lighting systems and devices including same |
WO2009151515A1 (en) | 2008-05-06 | 2009-12-17 | Qd Vision, Inc. | Solid state lighting devices including quantum confined semiconductor nanoparticles |
US10012375B1 (en) | 2008-05-20 | 2018-07-03 | Nader Salessi | Modular LED lamp |
US8230690B1 (en) | 2008-05-20 | 2012-07-31 | Nader Salessi | Modular LED lamp |
US8159152B1 (en) * | 2008-05-20 | 2012-04-17 | Nader Salessi | High-power LED lamp |
US20100030040A1 (en) | 2008-08-04 | 2010-02-04 | Masimo Laboratories, Inc. | Multi-stream data collection system for noninvasive measurement of blood constituents |
US8577431B2 (en) | 2008-07-03 | 2013-11-05 | Cercacor Laboratories, Inc. | Noise shielding for a noninvasive device |
US8304785B2 (en) * | 2008-07-29 | 2012-11-06 | Industrial Technology Research Institute | LED structure, manufacturing method thereof and LED module |
US8070324B2 (en) * | 2008-07-30 | 2011-12-06 | Mp Design Inc. | Thermal control system for a light-emitting diode fixture |
DE102008045653B4 (en) * | 2008-09-03 | 2020-03-26 | Osram Opto Semiconductors Gmbh | Optoelectronic component |
JP4711009B2 (en) * | 2008-10-16 | 2011-06-29 | ソニー株式会社 | Optical measuring device |
DE102008057347A1 (en) * | 2008-11-14 | 2010-05-20 | Osram Opto Semiconductors Gmbh | Optoelectronic device |
US8240885B2 (en) * | 2008-11-18 | 2012-08-14 | Abl Ip Holding Llc | Thermal management of LED lighting systems |
EP2206653A1 (en) * | 2008-12-09 | 2010-07-14 | Uhlmann Pac-Systeme GmbH & Co. KG | Device for checking the filling of cups in a packaging unit |
EP2384228A4 (en) * | 2009-01-05 | 2012-06-13 | Plextronics Inc | Organic light emitting diode phototherapy lighting system |
US20120046653A1 (en) * | 2009-03-05 | 2012-02-23 | Cynosure, Inc. | Pulsed therapeutic light system and method |
CN202830041U (en) * | 2009-04-03 | 2013-03-27 | Illumina公司 | Device for heating biological sample |
TWI447892B (en) * | 2009-04-20 | 2014-08-01 | Ind Tech Res Inst | Light emitting apparatus and fabrication method thereof |
US20120156766A1 (en) * | 2009-06-24 | 2012-06-21 | Akiko Shiratori | Sample analyzing chip and measurement system using same |
DE102009036066A1 (en) * | 2009-08-04 | 2011-02-10 | Carl Zeiss Microimaging Gmbh | Optoelectronic detector and method of operation for such |
WO2011031377A1 (en) | 2009-09-09 | 2011-03-17 | Helixis, Inc. | Optical system for multiple reactions |
US8602593B2 (en) * | 2009-10-15 | 2013-12-10 | Cree, Inc. | Lamp assemblies and methods of making the same |
TWI518736B (en) * | 2010-03-31 | 2016-01-21 | Ats自動模具系統股份有限公司 | Light generator systems and methods |
JP5050076B2 (en) * | 2010-04-13 | 2012-10-17 | 株式会社日立製作所 | Optical communication module and optical communication device |
US8297798B1 (en) | 2010-04-16 | 2012-10-30 | Cooper Technologies Company | LED lighting fixture |
JP2011237304A (en) * | 2010-05-11 | 2011-11-24 | Nippon Soken Inc | Fuel property measurement device, method for manufacturing fuel property measurement device and vehicle |
EP2615462B1 (en) * | 2010-11-15 | 2016-12-14 | F. Hoffmann-La Roche AG | Instrument and method for the automated thermal treatment of liquid samples |
DE202011001569U1 (en) * | 2011-01-14 | 2012-03-01 | Berthold Technologies Gmbh & Co. Kg | Device for measuring optical properties in microplates |
EP2511693A1 (en) | 2011-04-13 | 2012-10-17 | F. Hoffmann-La Roche AG | Analysis System with a spectrally controlled light source |
US8754593B2 (en) * | 2011-05-02 | 2014-06-17 | Tyco Electronics Corporation | Light emitting diode assembly having active cooling |
US20120300040A1 (en) * | 2011-05-25 | 2012-11-29 | Microsoft Corporation | Imaging system |
US20120300024A1 (en) * | 2011-05-25 | 2012-11-29 | Microsoft Corporation | Imaging system |
CN104114282B (en) | 2011-09-30 | 2017-07-04 | 生命技术公司 | For the system and method for bioanalysis |
EP2581728B1 (en) * | 2011-10-10 | 2013-09-18 | CYCLERtest B.V. | Calibration device for a thermal cycler |
JP5976128B2 (en) * | 2011-12-22 | 2016-08-23 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | Extending light source life in optical systems |
US9974630B2 (en) | 2012-04-13 | 2018-05-22 | Orthoaccel Technologies, Inc. | Laser orthodontic devices |
US9780518B2 (en) | 2012-04-18 | 2017-10-03 | Cynosure, Inc. | Picosecond laser apparatus and methods for treating target tissues with same |
US9907494B2 (en) | 2012-04-18 | 2018-03-06 | Hutchinson Technology Incorporated | NIRS device with optical wavelength and path length correction |
US9929325B2 (en) | 2012-06-05 | 2018-03-27 | Samsung Electronics Co., Ltd. | Lighting device including quantum dots |
JP5663541B2 (en) * | 2012-09-19 | 2015-02-04 | 株式会社日立ハイテクノロジーズ | Reaction vessel, parallel processing device, and sequencer |
US10591416B2 (en) * | 2013-02-22 | 2020-03-17 | Life Technologies Corporation | Optical systems and methods for biological analysis |
JP6151591B2 (en) * | 2013-02-22 | 2017-06-21 | 株式会社小糸製作所 | Vehicle lighting |
AU2013202788B2 (en) * | 2013-03-14 | 2015-10-01 | Gen-Probe Incorporated | Indexing signal detection module |
WO2014145707A2 (en) | 2013-03-15 | 2014-09-18 | Cynosure, Inc. | Picosecond optical radiation systems and methods of use |
JP6241191B2 (en) * | 2013-10-17 | 2017-12-06 | 株式会社サタケ | Lighting device for color sorter |
JP6241190B2 (en) * | 2013-10-17 | 2017-12-06 | 株式会社サタケ | Lighting device for color sorter |
GB2534753B (en) * | 2013-10-17 | 2020-06-17 | Satake Eng Co Ltd | Illumination device for color sorter |
EP2886936A1 (en) * | 2013-12-23 | 2015-06-24 | odelo GmbH | Lighting device and motor vehicle light equipped with same |
WO2015106024A1 (en) * | 2014-01-08 | 2015-07-16 | Vitec Videocom Inc. | Led heater system and method |
JP6755188B2 (en) * | 2014-03-07 | 2020-09-16 | ライフ テクノロジーズ コーポレーション | Optical system for capillary electrophoresis |
DE102014104240A1 (en) * | 2014-03-26 | 2015-10-01 | Sick Ag | Optical sensor |
JP6476856B2 (en) * | 2014-12-26 | 2019-03-06 | 日亜化学工業株式会社 | Display device, display |
JP6487214B2 (en) * | 2015-01-07 | 2019-03-20 | 旭化成エレクトロニクス株式会社 | Light emitting / receiving device and concentration measuring device |
CN113049553A (en) | 2015-02-06 | 2021-06-29 | 生命技术公司 | System and method for evaluating biological samples |
JP6545991B2 (en) * | 2015-03-31 | 2019-07-17 | 株式会社小糸製作所 | Light source module |
JP6638726B2 (en) * | 2015-03-31 | 2020-01-29 | ソニー株式会社 | Light source device, method of operating light source device, and medical system |
CA3158992C (en) * | 2015-06-09 | 2022-10-25 | Gen-Probe Incorporated | Methods and devices for calibrating and/or monitoring optical measurement devices |
DE102016200271A1 (en) * | 2016-01-13 | 2017-07-13 | Institut Dr. Foerster Gmbh & Co. Kg | Device for generating and measuring an emission |
GB201602502D0 (en) * | 2016-02-11 | 2016-03-30 | In Tandem Designs Pty Ltd | Light source |
WO2017161046A1 (en) * | 2016-03-15 | 2017-09-21 | Abbott Laboratories | Nucleic acid amplification and detection devices, systems and methods |
JP6544322B2 (en) * | 2016-09-05 | 2019-07-17 | 株式会社デンソー | Vehicle lamp controller |
JP6775799B2 (en) * | 2016-10-12 | 2020-10-28 | リョーエイ株式会社 | Oil film inspection method and oil film inspection device |
EP3312593A1 (en) * | 2016-10-20 | 2018-04-25 | Hain Lifescience GmbH | Method and device for exciting optically a plurality of analytes in an array of reaction vessels and for measuring the fluorescence from said analytes |
US20180136126A1 (en) * | 2016-11-17 | 2018-05-17 | Promega Corporation | Led-based illumination apparatus for configuration with a spectro-fluorometer system |
KR101800667B1 (en) * | 2016-12-23 | 2017-12-20 | (주)레이 | LCD Type 3D Printer |
JP2018141768A (en) * | 2017-02-24 | 2018-09-13 | パナソニックIpマネジメント株式会社 | Moisture amount sensor and clothing dryer |
JP7005362B2 (en) * | 2018-01-26 | 2022-02-04 | キヤノン株式会社 | Projection type display device |
ES2956109T3 (en) * | 2018-02-15 | 2023-12-13 | Procisedx Inc | Analyzer |
TWI660218B (en) * | 2018-02-22 | 2019-05-21 | 致茂電子股份有限公司 | Automatic fluorescence detection system |
WO2019165426A1 (en) | 2018-02-26 | 2019-08-29 | Cynosure, Inc. | Q-switched cavity dumped sub-nanosecond laser |
US11466190B2 (en) | 2018-06-25 | 2022-10-11 | Abb Schweiz Ag | Forced air cooling system with phase change material |
EP3627570A1 (en) * | 2018-09-18 | 2020-03-25 | Heraeus Additive Manufacturing GmbH | Heat exchanger for semiconductor elements |
GB2578920A (en) * | 2018-11-14 | 2020-06-03 | Duvas Tech Limited | Lighting arrangement for fluid analysis system |
JP7021717B2 (en) * | 2019-03-12 | 2022-02-17 | 株式会社島津製作所 | Spectrophotometer |
DE102019203318A1 (en) * | 2019-03-12 | 2020-09-17 | Robert Bosch Gmbh | Thermal regulation of a sensor device |
CN110793006A (en) * | 2019-10-25 | 2020-02-14 | 深圳市冠科科技有限公司 | Heat radiator and high-power electric light source |
JP7291063B2 (en) | 2019-11-20 | 2023-06-14 | 株式会社日立ハイテク | automatic analyzer |
CN111076103A (en) * | 2019-11-28 | 2020-04-28 | 中国科学院宁波材料技术与工程研究所 | Fluorescent module and laser lighting system |
DE102020106865A1 (en) | 2020-03-12 | 2021-09-16 | Analytik Jena Gmbh | Arrangement and method for PCR with multi-channel fluorescence measurement for spatially distributed samples |
US20220128566A1 (en) * | 2020-10-27 | 2022-04-28 | Quantum-Si Incorporated | Calibration of single-molecule detection system |
EP4283282A1 (en) * | 2021-01-22 | 2023-11-29 | Toho University | Autofluorescence quenching device |
DE102021133081B3 (en) | 2021-12-14 | 2023-05-04 | Bmg Labtech Gmbh | Microplate reader and method of making optical measurements with a microplate reader |
US20240027655A1 (en) * | 2022-07-19 | 2024-01-25 | Trustees Of Boston University | High throughput screening system for engineered cardiac tissues |
Citations (91)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3973129A (en) * | 1975-01-10 | 1976-08-03 | Bell Telephone Laboratories, Incorporated | Fluorimetric apparatus and method for analysis of body fluid |
US4284897A (en) * | 1977-04-30 | 1981-08-18 | Olympus Optical Company Ltd. | Fluorescence determining microscope utilizing laser light |
US4626684A (en) * | 1983-07-13 | 1986-12-02 | Landa Isaac J | Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples |
US4643877A (en) * | 1983-08-12 | 1987-02-17 | Max Planck Gesellschaft Zur Foerderung Der Wissenschaften | Fluorometer |
US4673289A (en) * | 1984-06-20 | 1987-06-16 | Commissariat A L'energie Atomique | Optical device with a high collection efficiency and cytofluorimeter making use of the same |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4762420A (en) * | 1986-04-01 | 1988-08-09 | Fisons Plc | Photometric reading device for serological analysis |
US4852985A (en) * | 1986-10-16 | 1989-08-01 | Olympus Optical Co., Ltd. | Illuminating device for microscopes |
US5073029A (en) * | 1990-02-16 | 1991-12-17 | Eqm Research, Inc. | Multisource device for photometric analysis and associated chromogens |
US5091652A (en) * | 1990-01-12 | 1992-02-25 | The Regents Of The University Of California | Laser excited confocal microscope fluorescence scanner and method |
US5166800A (en) * | 1990-03-26 | 1992-11-24 | Olympus Optical Co., Ltd. | Solid-state imaging device having a widened dynamic range |
US5169601A (en) * | 1990-04-27 | 1992-12-08 | Suzuki Motor Corporation | Immunological agglutination detecting apparatus with separately controlled supplementary light sources |
US5215883A (en) * | 1990-07-09 | 1993-06-01 | The Research Foundation | Electrophoretic mobility of fluorophore labeled particles in gels by fluorophore movement after photobleaching |
US5243540A (en) * | 1991-04-03 | 1993-09-07 | The United States Of America As Represented By The Secretary Of The Army | Computer-driven amino acid indexer for peptide synthesis |
US5256880A (en) * | 1991-01-31 | 1993-10-26 | Metallgesellschaft Aktiengesellschaft | Process for the qualitative analysis of plastic particles |
US5259381A (en) * | 1986-08-18 | 1993-11-09 | Physio-Control Corporation | Apparatus for the automatic calibration of signals employed in oximetry |
US5315375A (en) * | 1992-02-11 | 1994-05-24 | Acrogen, Inc. | Sensitive light detection system |
US5355215A (en) * | 1992-09-30 | 1994-10-11 | Environmental Research Institute Of Michigan | Method and apparatus for quantitative fluorescence measurements |
US5371016A (en) * | 1993-04-26 | 1994-12-06 | Becton, Dickinson And Company | Detecting biological activities in culture vials |
US5383023A (en) * | 1993-03-01 | 1995-01-17 | Walleczek; Jan | Method and apparatus for performing dual-beam dual-wavelength fluorescence spectrophotometric evaluation of a biological specimen |
US5389544A (en) * | 1990-02-21 | 1995-02-14 | Mitsubishi Jukogyo Kabushiki Kaisha | Method for counting living cells of microbes and apparatus therefor |
US5424841A (en) * | 1993-05-28 | 1995-06-13 | Molecular Dynamics | Apparatus for measuring spatial distribution of fluorescence on a substrate |
US5459325A (en) * | 1994-07-19 | 1995-10-17 | Molecular Dynamics, Inc. | High-speed fluorescence scanner |
US5475610A (en) * | 1990-11-29 | 1995-12-12 | The Perkin-Elmer Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
US5477853A (en) * | 1992-12-01 | 1995-12-26 | Somanetics Corporation | Temperature compensation method and apparatus for spectroscopic devices |
US5496517A (en) * | 1989-12-22 | 1996-03-05 | Beckman Instruments, Inc. | Laboratory workstation using thermal vaporization control |
US5547849A (en) * | 1993-02-17 | 1996-08-20 | Biometric Imaging, Inc. | Apparatus and method for volumetric capillary cytometry |
US5557398A (en) * | 1994-04-15 | 1996-09-17 | Molecular Devices Corporation | Photometric device |
US5567947A (en) * | 1995-06-01 | 1996-10-22 | Aerodyne Research, Inc. | Spectral line discriminator for passive detection of fluorescence |
US5595708A (en) * | 1993-08-27 | 1997-01-21 | Becton Dickinson And Company | System for detecting bacterial growth in a plurality of culture vials |
US5656493A (en) * | 1985-03-28 | 1997-08-12 | The Perkin-Elmer Corporation | System for automated performance of the polymerase chain reaction |
US5672880A (en) * | 1994-12-08 | 1997-09-30 | Molecular Dynamics, Inc. | Fluoresecence imaging system |
US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
US5759781A (en) * | 1995-12-22 | 1998-06-02 | Yale University | Multiparametric fluorescence in situ hybridization |
US5766889A (en) * | 1994-06-08 | 1998-06-16 | The Perkin-Elmer Corporation | Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample |
US5779978A (en) * | 1996-02-29 | 1998-07-14 | Avl Medical Instruments Ag | Measuring assembly for luminescence analysis |
US5792610A (en) * | 1996-05-01 | 1998-08-11 | Biorad Laboratories, Inc. | Method for conducting multiparametric fluorescence in situ hybridization |
US5846842A (en) * | 1993-05-18 | 1998-12-08 | University Of Utah Research Foundation | Waveguide immunosensor with coating chemistry and providing enhanced sensitivity |
US5854684A (en) * | 1996-09-26 | 1998-12-29 | Sarnoff Corporation | Massively parallel detection |
US5863502A (en) * | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
US5872623A (en) * | 1996-09-26 | 1999-02-16 | Sarnoff Corporation | Massively parallel detection |
US5926271A (en) * | 1995-12-20 | 1999-07-20 | Zeta Technology | Laser-induced fluorescence detector having a capillary detection cell and method for identifying trace compounds implemented by the same device |
US5943129A (en) * | 1997-08-07 | 1999-08-24 | Cambridge Research & Instrumentation Inc. | Fluorescence imaging system |
US6026323A (en) * | 1997-03-20 | 2000-02-15 | Polartechnics Limited | Tissue diagnostic system |
US6040940A (en) * | 1998-02-04 | 2000-03-21 | Olympus Optical Co., Ltd. | Reflecting fluorescence microscope |
US6057114A (en) * | 1991-12-20 | 2000-05-02 | Sibia Neurosciences, Inc. | Automated assays and methods for detecting and modulating cell surface protein function |
US6066245A (en) * | 1996-12-27 | 2000-05-23 | Genetic Biosystems, Inc. | Method and apparatus for scanning fluorescently labeled particles |
US6096272A (en) * | 1997-05-23 | 2000-08-01 | Becton Dickinson & Company | Automated microbiological testing apparatus and methods therefor |
US6154282A (en) * | 1998-10-26 | 2000-11-28 | Cytotelesis Inc. | Semiconductor based excitation illuminator for fluorescence and phosphorescence microscopy |
US6197575B1 (en) * | 1998-03-18 | 2001-03-06 | Massachusetts Institute Of Technology | Vascularized perfused microtissue/micro-organ arrays |
US6211989B1 (en) * | 1997-02-24 | 2001-04-03 | Bodenseewerk Perkin-Elmer Gmbh | Light-scanning device |
US6229635B1 (en) * | 1997-02-24 | 2001-05-08 | Bodenseewerk Perkin-Elmer Gmbh | Light sensing device |
US6287871B1 (en) * | 1996-03-19 | 2001-09-11 | University Of Utah Research Foundation | System for determining analyte concentration |
US20010033374A1 (en) * | 2000-02-25 | 2001-10-25 | Cambridge Research & Instrumentation Inc. | Multiple label fluorescence polarization assay system and method |
US6309601B1 (en) * | 1993-11-01 | 2001-10-30 | Nanogen, Inc. | Scanning optical detection system |
US6316774B1 (en) * | 1998-08-18 | 2001-11-13 | Molecular Devices Corporation | Optical system for a scanning fluorometer |
US6331438B1 (en) * | 1999-11-24 | 2001-12-18 | Iowa State University Research Foundation, Inc. | Optical sensors and multisensor arrays containing thin film electroluminescent devices |
US6331441B1 (en) * | 1996-12-31 | 2001-12-18 | Genometrix Genomics Incorporated | Multiplexed molecular analysis apparatus and method |
US6337740B1 (en) * | 1996-07-16 | 2002-01-08 | Caliper Technologies Corp. | Microfluidic devices for electrophoretic analysis of materials |
US6353475B1 (en) * | 1999-07-12 | 2002-03-05 | Caliper Technologies Corp. | Light source power modulation for use with chemical and biochemical analysis |
US6352672B1 (en) * | 1991-01-28 | 2002-03-05 | Cis Bio International | Apparatus for measuring the luminescence emitted in a luminescent assay |
US6355934B1 (en) * | 1999-02-26 | 2002-03-12 | Packard Biochip Technologies | Imaging system for an optical scanner |
US6364516B1 (en) * | 1997-06-30 | 2002-04-02 | Spectrumedix Corporation | Electrophoretic sample excitation light assembly |
US6377342B1 (en) * | 1995-09-04 | 2002-04-23 | Societe Francaise De Recherches Et D'investissements (Sfri) | Luminometer, particularly for medical assays |
US20020055178A1 (en) * | 1998-03-07 | 2002-05-09 | Wardlaw Stephen C. | Apparatus and method for analyzing biologic fluids |
US6388788B1 (en) * | 1998-03-16 | 2002-05-14 | Praelux, Inc. | Method and apparatus for screening chemical compounds |
US20020056804A1 (en) * | 2000-09-26 | 2002-05-16 | Fuji Photo Film Co., Ltd. | Light source device, image reading apparatus and image reading method |
US20020060791A1 (en) * | 1999-07-07 | 2002-05-23 | Ljl Biosystems, Inc. | Light detection device |
US6411835B1 (en) * | 1997-01-13 | 2002-06-25 | Medispectra, Inc. | Spectral volume microprobe arrays |
US20020109100A1 (en) * | 2000-10-27 | 2002-08-15 | Jackson Joseph H. | Light detection device |
US6455861B1 (en) * | 1998-11-24 | 2002-09-24 | Cambridge Research & Instrumentation, Inc. | Fluorescence polarization assay system and method |
US20020146688A1 (en) * | 1998-12-07 | 2002-10-10 | Olympus Optical Co., Ltd. | Method of analyzing a target nucleic acid |
US20020185610A1 (en) * | 1996-05-16 | 2002-12-12 | Affymetrix, Inc. | Systems and methods for detection of labeled materials |
US6519032B1 (en) * | 1998-04-03 | 2003-02-11 | Symyx Technologies, Inc. | Fiber optic apparatus and use thereof in combinatorial material science |
US6529275B2 (en) * | 2001-06-22 | 2003-03-04 | Biocal Technology, Inc. | Optical detection in bio-separation device using a widened detection zone |
US6563584B1 (en) * | 1999-05-11 | 2003-05-13 | Hitachi Software Engineering Co., Ltd. | Method and device for fluorescence measurement |
US6563581B1 (en) * | 2000-07-14 | 2003-05-13 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
US20030116497A1 (en) * | 2001-08-10 | 2003-06-26 | Carlson Eric D. | Apparatuses and methods for creating and testing pre-formulations and systems for same |
US6620623B1 (en) * | 2002-05-06 | 2003-09-16 | The University Of Chicago | Biochip reader with enhanced illumination and bioarray positioning apparatus |
US20030176776A1 (en) * | 2002-02-15 | 2003-09-18 | Matti Huiku | Compensation of human variability in pulse oximetry |
US6650411B2 (en) * | 2001-04-26 | 2003-11-18 | Affymetrix, Inc. | System, method, and product for pixel clocking in scanning of biological materials |
US20040009586A1 (en) * | 1998-05-16 | 2004-01-15 | Oldham Mark F. | Instrument for monitoring nucleic acid sequence amplification reaction |
US6686582B1 (en) * | 1997-10-31 | 2004-02-03 | Carl-Zeiss-Stiftung | Optical array system and reader for microtiter plates |
US20040207532A1 (en) * | 2003-04-18 | 2004-10-21 | Smithson Bradley D. | Temperature compensated warning light |
US6818437B1 (en) * | 1998-05-16 | 2004-11-16 | Applera Corporation | Instrument for monitoring polymerase chain reaction of DNA |
US20040253714A1 (en) * | 1994-02-10 | 2004-12-16 | Affymetrix, Inc. | Thermal and fluidic cycling device for nucleic acid hybridization |
US6852986B1 (en) * | 1999-11-12 | 2005-02-08 | E. I. Du Pont De Nemours And Company | Fluorometer with low heat-generating light source |
US20050057749A1 (en) * | 1998-08-21 | 2005-03-17 | Surromed, Inc. | Novel optical architectures for microvolume laser-scanning cytometers |
US20050279949A1 (en) * | 1999-05-17 | 2005-12-22 | Applera Corporation | Temperature control for light-emitting diode stabilization |
US7202953B1 (en) * | 1998-12-21 | 2007-04-10 | Evotec Biosystems Ag | Scanning microscopic method having high axial resolution |
US7423750B2 (en) * | 2001-11-29 | 2008-09-09 | Applera Corporation | Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion |
Family Cites Families (61)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57186169A (en) | 1981-05-12 | 1982-11-16 | Olympus Optical Co Ltd | Detector for particle coagulation pattern |
DE3441179A1 (en) | 1984-11-10 | 1986-05-22 | Dynatech Deutschland GmbH, 7306 Denkendorf | Temperature-control device for microcell arrangements, in particular microtitration plates |
JPH0645654B2 (en) | 1985-06-10 | 1994-06-15 | 博明 江川 | Chelate resin and manufacturing method thereof |
JPH0645654Y2 (en) | 1986-01-17 | 1994-11-24 | マツダ株式会社 | Engine intake system |
JPS62249778A (en) | 1986-04-23 | 1987-10-30 | Fuji Xerox Co Ltd | Electrophotographic image forming apparatus |
JPH07120393B2 (en) | 1986-04-24 | 1995-12-20 | 株式会社日立製作所 | Character recognition / graphic processing device |
GB8807297D0 (en) * | 1988-03-26 | 1988-04-27 | Dean P D G | Intelligent heating block |
JPH07120392B2 (en) | 1989-05-10 | 1995-12-20 | 三菱電機株式会社 | Character pattern cutting device |
JPH0645654A (en) * | 1991-01-23 | 1994-02-18 | Eastman Kodak Japan Kk | Light emitting device |
US5337740A (en) * | 1991-08-01 | 1994-08-16 | New England Pharmaceuticals, Inc. | Inhalation devices |
US5290904A (en) | 1991-07-31 | 1994-03-01 | Triangle Research And Development Corporation | Heat shield |
JPH06201468A (en) * | 1992-12-25 | 1994-07-19 | Hiroshi Maeda | Led light emitting spectroscope |
CA2129787A1 (en) | 1993-08-27 | 1995-02-28 | Russell G. Higuchi | Monitoring multiple amplification reactions simultaneously and analyzing same |
US5626936A (en) | 1993-09-09 | 1997-05-06 | Energy Pillow, Inc. | Phase change insulation system |
JPH07288351A (en) * | 1994-04-19 | 1995-10-31 | Fujitsu Ltd | Peltier control circuit and element structure thereof |
DE69519783T2 (en) | 1994-04-29 | 2001-06-07 | Perkin Elmer Corp | METHOD AND DEVICE FOR REAL-TIME DETECTION OF PRODUCTS OF NUCLEIC ACID AMPLIFICATION |
JPH07174701A (en) | 1994-12-28 | 1995-07-14 | Shimadzu Corp | Apparatus for determining base sequence |
US5784152A (en) | 1995-03-16 | 1998-07-21 | Bio-Rad Laboratories | Tunable excitation and/or tunable detection microplate reader |
US5679457A (en) | 1995-05-19 | 1997-10-21 | The Bergquist Company | Thermally conductive interface for electronic devices |
EP0866953A4 (en) * | 1995-08-22 | 2000-05-24 | Vivorx Pharmaceuticals Inc | Method and apparatus for determining characteristics of a sample in the presence of ambient light |
WO1997023649A1 (en) | 1995-12-22 | 1997-07-03 | Biorad Laboratories, Inc. | Method and apparatus for conducting multiparametric fluorescence in situ hybridization |
AU715627B2 (en) | 1996-02-21 | 2000-02-03 | Biomerieux Vitek, Inc. | Automatic sample testing machine |
JPH09281078A (en) * | 1996-04-09 | 1997-10-31 | Hitachi Electron Eng Co Ltd | Dna base sequence determining apparatus |
JPH09304276A (en) * | 1996-05-18 | 1997-11-28 | Kyokuto Sanki Co Ltd | Apparatus for measuring contamination degree of carpet |
EP0912760B1 (en) | 1996-06-04 | 2005-11-09 | University Of Utah Research Foundation | System and methods for monitoring for dna amplification by fluorescence |
US5844208A (en) * | 1997-04-04 | 1998-12-01 | Unisys Corporation | Temperature control system for an electronic device in which device temperature is estimated from heater temperature and heat sink temperature |
GB9717021D0 (en) * | 1997-08-12 | 1997-10-15 | Kalibrant Limited | A detector |
US6043506A (en) * | 1997-08-13 | 2000-03-28 | Bio-Rad Laboratories, Inc. | Multi parameter scanner |
US6061128A (en) | 1997-09-04 | 2000-05-09 | Avocet Medical, Inc. | Verification device for optical clinical assay systems |
US6597450B1 (en) | 1997-09-15 | 2003-07-22 | Becton, Dickinson And Company | Automated Optical Reader for Nucleic Acid Assays |
US6077597A (en) | 1997-11-14 | 2000-06-20 | Outlast Technologies, Inc. | Interactive thermal insulating system having a layer treated with a coating of energy absorbing phase change material adjacent a layer of fibers containing energy absorbing phase change material |
JP3813730B2 (en) | 1998-03-19 | 2006-08-23 | 浜松ホトニクス株式会社 | Fluorescence measuring device |
US6040586A (en) | 1998-05-05 | 2000-03-21 | The Perkin-Elmer Corporation | Method and system for velocity-normalized position-based scanning |
EP3093649B1 (en) | 1998-05-16 | 2019-05-08 | Life Technologies Corporation | A combination of a reaction apparatus and an optical instrument monitoring dna polymerase chain reactions |
DE19940749A1 (en) | 1998-08-28 | 2000-05-18 | Febit Ferrarius Biotech Gmbh | Integrated synthesis and analysis method e.g. for polymers, comprises a carrier body provided with immobilized receptors to provide respective channels before contact with sample and subsequent analysis |
JP2000121559A (en) | 1998-10-14 | 2000-04-28 | Hitachi Denshi Ltd | Device for reading quantity of light of micro spot |
JP3585753B2 (en) * | 1998-12-15 | 2004-11-04 | 富士写真フイルム株式会社 | Shooting system |
GB9906929D0 (en) | 1999-03-26 | 1999-05-19 | Univ Glasgow | Assay system |
US7119345B2 (en) * | 2003-02-28 | 2006-10-10 | Applera Corporation | Excitation and emission filter |
US7387891B2 (en) * | 1999-05-17 | 2008-06-17 | Applera Corporation | Optical instrument including excitation source |
DE19925161A1 (en) | 1999-06-02 | 2000-12-07 | Stratec Biomedical Systems Ag | Performing serial luminometric assays, useful e.g. for analysis of nucleic acid sequences, comprises automated and sequential transfer of sample cells to measuring station |
WO2001013096A1 (en) | 1999-08-13 | 2001-02-22 | Zeptosens Ag | Device and method for determining multiple analytes |
JP2001083090A (en) * | 1999-09-10 | 2001-03-30 | Fuji Photo Film Co Ltd | Excitation light source for micro titer plate |
JP3551860B2 (en) | 1999-10-05 | 2004-08-11 | 株式会社日立製作所 | DNA testing method and DNA testing device |
EP1228357B1 (en) | 1999-11-12 | 2008-05-28 | E. I. du Pont de Nemours and Company | Fluorometer with low heat-generating light source |
NL1014260C2 (en) * | 2000-02-02 | 2001-08-03 | Lely Entpr Ag | Device for detecting physical abnormalities in milk. |
US6704104B2 (en) | 2000-03-16 | 2004-03-09 | Spectrumedix Llc | Multi-wavelength array reader for biological assay |
JP2001346002A (en) * | 2000-06-05 | 2001-12-14 | Fuji Photo Film Co Ltd | Light source device and image reader |
US20030160957A1 (en) * | 2000-07-14 | 2003-08-28 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
JP2002116141A (en) * | 2000-08-01 | 2002-04-19 | Society For Techno-Innovation Of Agriculture Forestry & Fisheries | Handy non-destructive measuring apparatus for component of fruit |
WO2002026911A1 (en) | 2000-09-27 | 2002-04-04 | Microtek Laboratories, Inc. | Macrocapsules containing microencapsulated phase change materials |
JP2002116508A (en) * | 2000-10-04 | 2002-04-19 | Noritsu Koki Co Ltd | Image forming device |
US6709857B2 (en) | 2001-06-26 | 2004-03-23 | Becton, Dickinson And Company | System and method for optically monitoring the concentration of a gas in a sample vial using photothermal spectroscopy to detect sample growth |
US6744502B2 (en) | 2001-09-28 | 2004-06-01 | Pe Corporation (Ny) | Shaped illumination geometry and intensity using a diffractive optical element |
JP2004247312A (en) * | 2001-11-09 | 2004-09-02 | Ccs Inc | Light source device |
US7635588B2 (en) * | 2001-11-29 | 2009-12-22 | Applied Biosystems, Llc | Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength |
US6645598B2 (en) | 2002-01-04 | 2003-11-11 | Robert J. Alderman | Cell insulation blanket with phase change material, and method of making |
US7211299B2 (en) * | 2003-01-09 | 2007-05-01 | Con-Trol-Cure, Inc. | UV curing method and apparatus |
JP2004219322A (en) * | 2003-01-16 | 2004-08-05 | Astem:Kk | Non-destructive spectrophotometric instrument |
JP3874188B2 (en) * | 2003-02-13 | 2007-01-31 | ノーリツ鋼機株式会社 | LED light source temperature control device |
JP4565631B2 (en) | 2005-01-20 | 2010-10-20 | 日本電信電話株式会社 | Secret calculation method and system, and program |
-
2004
- 2004-11-04 US US10/981,440 patent/US20050279949A1/en not_active Abandoned
-
2005
- 2005-11-03 EP EP07120202A patent/EP1890182A2/en not_active Withdrawn
- 2005-11-03 JP JP2007539342A patent/JP2008519266A/en active Pending
- 2005-11-03 EP EP05849156.4A patent/EP1807726B1/en not_active Not-in-force
- 2005-11-03 EP EP20100075604 patent/EP2306233A3/en not_active Withdrawn
- 2005-11-03 WO PCT/US2005/039839 patent/WO2006052682A2/en active Application Filing
-
2006
- 2006-12-22 US US11/644,410 patent/US20070105212A1/en not_active Abandoned
-
2010
- 2010-09-30 US US12/895,848 patent/US20110159549A1/en not_active Abandoned
- 2010-12-24 JP JP2010288958A patent/JP2011059134A/en not_active Withdrawn
-
2012
- 2012-09-14 US US13/619,679 patent/US9285318B2/en not_active Expired - Fee Related
-
2014
- 2014-02-12 JP JP2014024057A patent/JP2014081391A/en not_active Withdrawn
-
2016
- 2016-01-08 JP JP2016002231A patent/JP6050529B2/en active Active
- 2016-03-09 US US15/065,834 patent/US20160305881A1/en not_active Abandoned
Patent Citations (96)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3973129A (en) * | 1975-01-10 | 1976-08-03 | Bell Telephone Laboratories, Incorporated | Fluorimetric apparatus and method for analysis of body fluid |
US4284897A (en) * | 1977-04-30 | 1981-08-18 | Olympus Optical Company Ltd. | Fluorescence determining microscope utilizing laser light |
US4626684A (en) * | 1983-07-13 | 1986-12-02 | Landa Isaac J | Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples |
US4643877A (en) * | 1983-08-12 | 1987-02-17 | Max Planck Gesellschaft Zur Foerderung Der Wissenschaften | Fluorometer |
US4673289A (en) * | 1984-06-20 | 1987-06-16 | Commissariat A L'energie Atomique | Optical device with a high collection efficiency and cytofluorimeter making use of the same |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5656493A (en) * | 1985-03-28 | 1997-08-12 | The Perkin-Elmer Corporation | System for automated performance of the polymerase chain reaction |
US4683202B1 (en) * | 1985-03-28 | 1990-11-27 | Cetus Corp | |
US4762420A (en) * | 1986-04-01 | 1988-08-09 | Fisons Plc | Photometric reading device for serological analysis |
US5259381A (en) * | 1986-08-18 | 1993-11-09 | Physio-Control Corporation | Apparatus for the automatic calibration of signals employed in oximetry |
US4852985A (en) * | 1986-10-16 | 1989-08-01 | Olympus Optical Co., Ltd. | Illuminating device for microscopes |
US5496517A (en) * | 1989-12-22 | 1996-03-05 | Beckman Instruments, Inc. | Laboratory workstation using thermal vaporization control |
US5091652A (en) * | 1990-01-12 | 1992-02-25 | The Regents Of The University Of California | Laser excited confocal microscope fluorescence scanner and method |
US5073029A (en) * | 1990-02-16 | 1991-12-17 | Eqm Research, Inc. | Multisource device for photometric analysis and associated chromogens |
US5389544A (en) * | 1990-02-21 | 1995-02-14 | Mitsubishi Jukogyo Kabushiki Kaisha | Method for counting living cells of microbes and apparatus therefor |
US5166800A (en) * | 1990-03-26 | 1992-11-24 | Olympus Optical Co., Ltd. | Solid-state imaging device having a widened dynamic range |
US5169601A (en) * | 1990-04-27 | 1992-12-08 | Suzuki Motor Corporation | Immunological agglutination detecting apparatus with separately controlled supplementary light sources |
US5215883A (en) * | 1990-07-09 | 1993-06-01 | The Research Foundation | Electrophoretic mobility of fluorophore labeled particles in gels by fluorophore movement after photobleaching |
US5475610A (en) * | 1990-11-29 | 1995-12-12 | The Perkin-Elmer Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
US6352672B1 (en) * | 1991-01-28 | 2002-03-05 | Cis Bio International | Apparatus for measuring the luminescence emitted in a luminescent assay |
US5256880A (en) * | 1991-01-31 | 1993-10-26 | Metallgesellschaft Aktiengesellschaft | Process for the qualitative analysis of plastic particles |
US5243540A (en) * | 1991-04-03 | 1993-09-07 | The United States Of America As Represented By The Secretary Of The Army | Computer-driven amino acid indexer for peptide synthesis |
US6057114A (en) * | 1991-12-20 | 2000-05-02 | Sibia Neurosciences, Inc. | Automated assays and methods for detecting and modulating cell surface protein function |
US5315375A (en) * | 1992-02-11 | 1994-05-24 | Acrogen, Inc. | Sensitive light detection system |
US5355215A (en) * | 1992-09-30 | 1994-10-11 | Environmental Research Institute Of Michigan | Method and apparatus for quantitative fluorescence measurements |
US5477853A (en) * | 1992-12-01 | 1995-12-26 | Somanetics Corporation | Temperature compensation method and apparatus for spectroscopic devices |
US5547849A (en) * | 1993-02-17 | 1996-08-20 | Biometric Imaging, Inc. | Apparatus and method for volumetric capillary cytometry |
US5383023A (en) * | 1993-03-01 | 1995-01-17 | Walleczek; Jan | Method and apparatus for performing dual-beam dual-wavelength fluorescence spectrophotometric evaluation of a biological specimen |
US5371016A (en) * | 1993-04-26 | 1994-12-06 | Becton, Dickinson And Company | Detecting biological activities in culture vials |
US5846842A (en) * | 1993-05-18 | 1998-12-08 | University Of Utah Research Foundation | Waveguide immunosensor with coating chemistry and providing enhanced sensitivity |
US5424841A (en) * | 1993-05-28 | 1995-06-13 | Molecular Dynamics | Apparatus for measuring spatial distribution of fluorescence on a substrate |
US5595708A (en) * | 1993-08-27 | 1997-01-21 | Becton Dickinson And Company | System for detecting bacterial growth in a plurality of culture vials |
US6309601B1 (en) * | 1993-11-01 | 2001-10-30 | Nanogen, Inc. | Scanning optical detection system |
US20040253714A1 (en) * | 1994-02-10 | 2004-12-16 | Affymetrix, Inc. | Thermal and fluidic cycling device for nucleic acid hybridization |
US5557398A (en) * | 1994-04-15 | 1996-09-17 | Molecular Devices Corporation | Photometric device |
US5766889A (en) * | 1994-06-08 | 1998-06-16 | The Perkin-Elmer Corporation | Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample |
US5459325A (en) * | 1994-07-19 | 1995-10-17 | Molecular Dynamics, Inc. | High-speed fluorescence scanner |
US5672880A (en) * | 1994-12-08 | 1997-09-30 | Molecular Dynamics, Inc. | Fluoresecence imaging system |
US5567947A (en) * | 1995-06-01 | 1996-10-22 | Aerodyne Research, Inc. | Spectral line discriminator for passive detection of fluorescence |
US6377342B1 (en) * | 1995-09-04 | 2002-04-23 | Societe Francaise De Recherches Et D'investissements (Sfri) | Luminometer, particularly for medical assays |
US5926271A (en) * | 1995-12-20 | 1999-07-20 | Zeta Technology | Laser-induced fluorescence detector having a capillary detection cell and method for identifying trace compounds implemented by the same device |
US5759781A (en) * | 1995-12-22 | 1998-06-02 | Yale University | Multiparametric fluorescence in situ hybridization |
US5863502A (en) * | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
US5779978A (en) * | 1996-02-29 | 1998-07-14 | Avl Medical Instruments Ag | Measuring assembly for luminescence analysis |
US6287871B1 (en) * | 1996-03-19 | 2001-09-11 | University Of Utah Research Foundation | System for determining analyte concentration |
US5792610A (en) * | 1996-05-01 | 1998-08-11 | Biorad Laboratories, Inc. | Method for conducting multiparametric fluorescence in situ hybridization |
US20020185610A1 (en) * | 1996-05-16 | 2002-12-12 | Affymetrix, Inc. | Systems and methods for detection of labeled materials |
US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
US6337740B1 (en) * | 1996-07-16 | 2002-01-08 | Caliper Technologies Corp. | Microfluidic devices for electrophoretic analysis of materials |
US5872623A (en) * | 1996-09-26 | 1999-02-16 | Sarnoff Corporation | Massively parallel detection |
US5854684A (en) * | 1996-09-26 | 1998-12-29 | Sarnoff Corporation | Massively parallel detection |
US6066245A (en) * | 1996-12-27 | 2000-05-23 | Genetic Biosystems, Inc. | Method and apparatus for scanning fluorescently labeled particles |
US6331441B1 (en) * | 1996-12-31 | 2001-12-18 | Genometrix Genomics Incorporated | Multiplexed molecular analysis apparatus and method |
US6411835B1 (en) * | 1997-01-13 | 2002-06-25 | Medispectra, Inc. | Spectral volume microprobe arrays |
US6229635B1 (en) * | 1997-02-24 | 2001-05-08 | Bodenseewerk Perkin-Elmer Gmbh | Light sensing device |
US6211989B1 (en) * | 1997-02-24 | 2001-04-03 | Bodenseewerk Perkin-Elmer Gmbh | Light-scanning device |
US6026323A (en) * | 1997-03-20 | 2000-02-15 | Polartechnics Limited | Tissue diagnostic system |
US6096272A (en) * | 1997-05-23 | 2000-08-01 | Becton Dickinson & Company | Automated microbiological testing apparatus and methods therefor |
US6364516B1 (en) * | 1997-06-30 | 2002-04-02 | Spectrumedix Corporation | Electrophoretic sample excitation light assembly |
US5943129A (en) * | 1997-08-07 | 1999-08-24 | Cambridge Research & Instrumentation Inc. | Fluorescence imaging system |
US6686582B1 (en) * | 1997-10-31 | 2004-02-03 | Carl-Zeiss-Stiftung | Optical array system and reader for microtiter plates |
US6040940A (en) * | 1998-02-04 | 2000-03-21 | Olympus Optical Co., Ltd. | Reflecting fluorescence microscope |
US6929953B1 (en) * | 1998-03-07 | 2005-08-16 | Robert A. Levine | Apparatus for analyzing biologic fluids |
US20020055178A1 (en) * | 1998-03-07 | 2002-05-09 | Wardlaw Stephen C. | Apparatus and method for analyzing biologic fluids |
US6388788B1 (en) * | 1998-03-16 | 2002-05-14 | Praelux, Inc. | Method and apparatus for screening chemical compounds |
US6197575B1 (en) * | 1998-03-18 | 2001-03-06 | Massachusetts Institute Of Technology | Vascularized perfused microtissue/micro-organ arrays |
US6519032B1 (en) * | 1998-04-03 | 2003-02-11 | Symyx Technologies, Inc. | Fiber optic apparatus and use thereof in combinatorial material science |
US6818437B1 (en) * | 1998-05-16 | 2004-11-16 | Applera Corporation | Instrument for monitoring polymerase chain reaction of DNA |
US20040009586A1 (en) * | 1998-05-16 | 2004-01-15 | Oldham Mark F. | Instrument for monitoring nucleic acid sequence amplification reaction |
US6316774B1 (en) * | 1998-08-18 | 2001-11-13 | Molecular Devices Corporation | Optical system for a scanning fluorometer |
US20050057749A1 (en) * | 1998-08-21 | 2005-03-17 | Surromed, Inc. | Novel optical architectures for microvolume laser-scanning cytometers |
US6154282A (en) * | 1998-10-26 | 2000-11-28 | Cytotelesis Inc. | Semiconductor based excitation illuminator for fluorescence and phosphorescence microscopy |
US6455861B1 (en) * | 1998-11-24 | 2002-09-24 | Cambridge Research & Instrumentation, Inc. | Fluorescence polarization assay system and method |
US20020146688A1 (en) * | 1998-12-07 | 2002-10-10 | Olympus Optical Co., Ltd. | Method of analyzing a target nucleic acid |
US7202953B1 (en) * | 1998-12-21 | 2007-04-10 | Evotec Biosystems Ag | Scanning microscopic method having high axial resolution |
US6355934B1 (en) * | 1999-02-26 | 2002-03-12 | Packard Biochip Technologies | Imaging system for an optical scanner |
US6563584B1 (en) * | 1999-05-11 | 2003-05-13 | Hitachi Software Engineering Co., Ltd. | Method and device for fluorescence measurement |
US7599060B2 (en) * | 1999-05-17 | 2009-10-06 | Applied Biosystems, Llc | Optical scanning configurations, systems, and methods involving at least one actuator for scanning a scan head |
US20070105212A1 (en) * | 1999-05-17 | 2007-05-10 | Applera Corporation | Temperature control for light-emitting diode stabilization |
US20050279949A1 (en) * | 1999-05-17 | 2005-12-22 | Applera Corporation | Temperature control for light-emitting diode stabilization |
US20020060791A1 (en) * | 1999-07-07 | 2002-05-23 | Ljl Biosystems, Inc. | Light detection device |
US6353475B1 (en) * | 1999-07-12 | 2002-03-05 | Caliper Technologies Corp. | Light source power modulation for use with chemical and biochemical analysis |
US6852986B1 (en) * | 1999-11-12 | 2005-02-08 | E. I. Du Pont De Nemours And Company | Fluorometer with low heat-generating light source |
US6331438B1 (en) * | 1999-11-24 | 2001-12-18 | Iowa State University Research Foundation, Inc. | Optical sensors and multisensor arrays containing thin film electroluminescent devices |
US20010033374A1 (en) * | 2000-02-25 | 2001-10-25 | Cambridge Research & Instrumentation Inc. | Multiple label fluorescence polarization assay system and method |
US6563581B1 (en) * | 2000-07-14 | 2003-05-13 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
US20020056804A1 (en) * | 2000-09-26 | 2002-05-16 | Fuji Photo Film Co., Ltd. | Light source device, image reading apparatus and image reading method |
US6960759B2 (en) * | 2000-09-26 | 2005-11-01 | Fuji Photo Film Co., Ltd. | Light source device, image reading apparatus and image reading method |
US20020109100A1 (en) * | 2000-10-27 | 2002-08-15 | Jackson Joseph H. | Light detection device |
US6650411B2 (en) * | 2001-04-26 | 2003-11-18 | Affymetrix, Inc. | System, method, and product for pixel clocking in scanning of biological materials |
US6529275B2 (en) * | 2001-06-22 | 2003-03-04 | Biocal Technology, Inc. | Optical detection in bio-separation device using a widened detection zone |
US20030116497A1 (en) * | 2001-08-10 | 2003-06-26 | Carlson Eric D. | Apparatuses and methods for creating and testing pre-formulations and systems for same |
US7423750B2 (en) * | 2001-11-29 | 2008-09-09 | Applera Corporation | Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion |
US20030176776A1 (en) * | 2002-02-15 | 2003-09-18 | Matti Huiku | Compensation of human variability in pulse oximetry |
US6620623B1 (en) * | 2002-05-06 | 2003-09-16 | The University Of Chicago | Biochip reader with enhanced illumination and bioarray positioning apparatus |
US20040207532A1 (en) * | 2003-04-18 | 2004-10-21 | Smithson Bradley D. | Temperature compensated warning light |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050279949A1 (en) * | 1999-05-17 | 2005-12-22 | Applera Corporation | Temperature control for light-emitting diode stabilization |
US20070105212A1 (en) * | 1999-05-17 | 2007-05-10 | Applera Corporation | Temperature control for light-emitting diode stabilization |
US9285318B2 (en) | 1999-05-17 | 2016-03-15 | Applied Biosystems, Llc | Optical instrument including excitation source |
US8900282B2 (en) | 2005-02-17 | 2014-12-02 | Biolux Research Ltd. | Light therapy apparatus and methods |
US9308389B2 (en) | 2005-02-17 | 2016-04-12 | Biolux Research Ltd. | Light therapy apparatus and methods |
CN105451635A (en) * | 2013-08-23 | 2016-03-30 | 奥林巴斯株式会社 | Light source device and endoscope device |
US9730780B2 (en) | 2013-10-22 | 2017-08-15 | Biolux Research Ltd. | Intra-oral light-therapy apparatuses and methods for their use |
US10729524B2 (en) | 2013-10-22 | 2020-08-04 | Biolux Research Holdings, Inc. | Intra-oral light-therapy apparatuses and methods for their use |
CN105987314A (en) * | 2015-05-16 | 2016-10-05 | 深圳市金达照明有限公司 | Lamp capable of automatically adjusting light source temperature |
CN105351899A (en) * | 2015-09-23 | 2016-02-24 | 华南理工大学 | LED heat-dissipating device adopting semiconductor refrigerating plate and phase change materials |
CN111788867A (en) * | 2018-06-15 | 2020-10-16 | 伊诺瓦半导体有限责任公司 | Method and system device for setting constant wavelength |
Also Published As
Publication number | Publication date |
---|---|
EP2306233A3 (en) | 2011-07-20 |
EP2306233A2 (en) | 2011-04-06 |
WO2006052682A3 (en) | 2006-08-03 |
US9285318B2 (en) | 2016-03-15 |
JP2014081391A (en) | 2014-05-08 |
US20160305881A1 (en) | 2016-10-20 |
JP2008519266A (en) | 2008-06-05 |
EP1807726B1 (en) | 2016-04-27 |
WO2006052682A2 (en) | 2006-05-18 |
US20070105212A1 (en) | 2007-05-10 |
JP2016076501A (en) | 2016-05-12 |
US20050279949A1 (en) | 2005-12-22 |
EP1890182A2 (en) | 2008-02-20 |
EP1807726A2 (en) | 2007-07-18 |
JP2011059134A (en) | 2011-03-24 |
US20130034859A1 (en) | 2013-02-07 |
JP6050529B2 (en) | 2016-12-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20110159549A1 (en) | Temperature control for light-emitting diode stabilization | |
US7599060B2 (en) | Optical scanning configurations, systems, and methods involving at least one actuator for scanning a scan head | |
EP2555873B1 (en) | Biochemical reactions system | |
EP1743384B1 (en) | Led array having array-based led detectors | |
US7628507B2 (en) | Radiance output and temperature controlled LED radiance source | |
EP2795271B1 (en) | Light source lifetime extension in an optical system | |
US20100203595A1 (en) | Thermal cycling apparatus and process | |
EP0101468A1 (en) | Infrared radiation source arrangement | |
US20060289786A1 (en) | System and method for a pulsed light source used in fluorescence detection | |
WO2007138302A1 (en) | Performance issues in use of vessels for biological applications | |
CN111013688B (en) | qPCR module and modularized qPCR device | |
JP2013544496A (en) | Thermal cycler | |
JP2021166184A (en) | Cooled photomultiplier tube-based photodetector with reduced condensation and related device and method | |
Gu et al. | White LED performance | |
CN214781858U (en) | Temperature control system and gene amplification equipment | |
JPH1137933A (en) | Absorptiometer | |
Ando et al. | LED-based light source for ultraviolet-visible absorption spectrophotometry | |
GB2578920A (en) | Lighting arrangement for fluid analysis system | |
WO2010010361A1 (en) | Improvements in reactor apparatus | |
HU202981B (en) | Measuring arrangement for measuring physical characteristics of the solid, liquid or gaseous material in optical way |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: LIFE TECHNOLOGIES CORPORATION, CALIFORNIA Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:APPLIED BIOSYSTEMS, LLC;REEL/FRAME:035051/0313 Effective date: 20150227 |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |