US20080115244A1

US20080115244A1 - Method for producing immunoglobulins containing protection proteins and their use

Info

Publication number: US20080115244A1
Application number: US11/605,720
Authority: US
Inventors: Andrew C. Hiatt; Julian K.-C. Ma; Thomas Lehner
Original assignee: University of California
Current assignee: University of California
Priority date: 1994-12-30
Filing date: 2006-11-28
Publication date: 2008-05-15
Also published as: US6808709B1; US7211389B2; US20050202026A1

Abstract

The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment.

The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules.

The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

Description

CROSS REFERENCE TO RELATED APPLICATIONS

This is a continuation of co-pending application Ser. No. 08/434,000 filed May 4, 1995, which is a continuation-in-part of co-pending application Ser. No. 08/367,395 filed Dec. 30, 1994, each of which is hereby incorporated by reference in its entirety including drawings.

FIELD OF INVENTION

The present invention relates to expression of immunoglobulins in plants that contain a protection protein as well as to transgenic plants that express such immunoglobulins. The therapeutic use of these immunoglobulins is also contemplated.

BACKGROUND TO THE INVENTION

Monoclonal antibodies have great potential for numerous therapeutic purposes. The advantages of monoclonal antibody therapeutics over conventional pharmaceuticals include their exquisite selectivity, multiple effector functions, and ease of molecular manipulation such as radioisotope labelling and other types of conjugation. A wide variety of target antigens have been used to generate specific monoclonal antibodies. See for example Therapeutic Monoclonal Antibodies, C. A. K. Borrebaeck and J. W. Larrick eds., Stockton Press, New York, 1990, and The Pharmacology of Monoclonal Antibodies, M. Rosenberg and G. P. Moore eds., Springer-Verlag, Berlin, 1994.
One therapeutic application of monoclonal antibodies is passive immunotherapy in which the exogenously produced immunoglobulins are administered directly to the animal being treated by injection or by ingestion. To be successful, passive immunotherapy must deliver an appropriate amount of an immunoglobulin to the animal, because passive immunotherapy does not rely on an immune response in the animal being treated. The immunoglobulins administered must be specific for the pathogen or molecule desired to effect treatment. One advantage of passive immunotherapy is the speed at which the antibody can be contacted with the target compared to a normal immune response. Passive immunotherapy can also be used as a prophylaxis to prevent the onset of diseases or infections.
A major potential use of passive immunotherapy is in combating bacterial infections. Recent emergence of anti-biotic resistant bacteria make treatment of bacterial infections with passive immunotherapy desirable. Antibiotic treatment targeted to a single pathogen often involves eradication of a large population of normal microbes, and this can have undesired side effects. An alternative approach has been to utilize the inherent specificity of immunoglobulins to inhibit a specific pathogenic function in very specific microbial populations. In this strategy, purified immunoglobulins of the appropriate specificity would be administered in order to provide a passive barrier to pathogen invasion.
In addition, the immunoglobulins used for passive immunotherapies for example, for oral administration of immunoglobulins must meet certain requirements. First, the immunoglobulin must be functional in very harsh environments, such as the gastrointestinal tract. Second, the immunoglobulin must be resistant to the actions of proteases so that it will not be degraded prior to inactivating the target.
Certain types of cells, including epithelial cells and hepatocytes, are capable of assembling immunoglobulin molecules which have been specifically adapted to function in harsh environments. These immunoglobulins are referred to as secretory immunoglobulins (SIg) and include both secretory IgA (SIgA) and secretory IgM (SIgM). The protection provided by endogenous secretory immunoglobulins have been demonstrated. Several mechanisms for protection from bacterial infection by secretory immunoglobulins have been proposed, including, but not limited to, direct killing, agglutination, inhibition of epithelial attachment and invasion, inactivation of enzymes and toxins, opsonization and complement activation. In an animal, endogenously produced SIgA are exposed to very harsh environments where numerous proteases, such as intestinal and bacterial enzymes are extremely active and denaturants, such as stomach acid, are also present.
One component of secretory immunoglobulins, the secretory component, helps to protect the immunoglobulin against these inactivating agents thereby increasing the biological effectiveness of secretory immunoglobulin.
The mechanism of synthesis and assembly of these secretory immunoglobulins, such as SIgA or SIgM is extremely complex. In animal cells, secretory immunoglobulins are assembled in a process involving different cell types. Each secretory immunoglobulin is made up of immunoglobulin heavy and light chains, joining chain (J chain) and a secretory component. The immunoglobulin producing B cells make and assemble the immunoglobulin heavy and light chain together with J chain to produce dimeric or polymeric IgM or IgA. The secretory component is produced by a second type of cell, either epithelial cells or hepatocytes, and secretory immunoglobulin is assembled in and secreted from these cells. The mechanism by which these cells assemble and secrete the secretory immunoglobulin is extremely complex and requires a unique microenvironment provided, for example, by mucosal tissues. The microenvironment places the B cells that produce the polymeric immunoglobulin near the cells that assemble and secrete secretory immunoglobulin onto the mucosal surface of an animal.
The epithelial cells have a receptor, the polyimmunoglobulin receptor (pIgR), that specifically recognizes and binds polymeric immunoglobulin/containing J chain, internalizing it and transporting it through the epithelial cell. Expressed on the basolateral cell surface, the pIgR has an N-terminal signal peptide of 18 amino acids, an extracellular polyimmunoglobulin binding portion of 629 amino acids, a membrane spanning segment of 23 hydrophobic residues, and a cytoplasmic tail of 103 amino acids. The extra-cellular portion contains five immunoglobulin-like domains of 100-111 amino acids each and constitutes the secreted form of the molecule. See for example, Mostov, Ann. Rev. Immol., 12:63-84 (1994) The site at which the polyimmunoglobulin receptor is cleaved to generate mature secretory component has not been accurately determined.
The polyimmunoglobulin receptor is located on the basolateral surface of epithelial cells in animals. Polymeric J chain-containing immunoglobulins produced in B cells interact with and are bound by the receptor resulting in vesicularization, transport across the epithelial cell, and ultimate secretion to the mucosal surface. Transepithelial transport also involves proteolysis and phosphorylation to produce the mature SIg containing the secretory component. The close association of the required cells found in the mucosal microenvironment, specifically the B lymphocytes and epithelial cells, is required for secretory immunoglobulin assembly.
The targeting of the production of immunoglobulins in transgenic organisms, such as mice, is extremely difficult and transgenic organisms made from fungus or plants do not contain the proper cell types and mucosal microenvironment to produce secretory immunoglobulins. The production of large amounts of secretory immunoglobulins in transgenic organisms and cell culture has, before this invention, been impossible. One desiring to produce a secretory immunoglobulin in cell culture or a transgenic organism must express the immunoglobulin heavy chain, the immunoglobulin light chain, and J chain in a B lymphocyte. To mimic the proper mucosal microenvironment a cell having the pIgR receptor on its surface would also have to be present and be in close association with that B lymphocyte to even attempt to assemble a functional secretory immunoglobulin.
This elaborate process required for natural secretory immunoglobulin assembly is extremely difficult to duplicate in cell culture or transgenic organisms. Production of SIg in cell culture or transgenic organisms would require coupling the functions of cells producing immunoglobulin with the functions of epithelial cells in artificial (in vitro) systems. Moreover, if the desired transgenic organism is a fungus, a bacterium, or a plant, the cell types and pathways of receptor-mediated cellular internalization, transcytosis, and secretion simply are not present. Those organisms lack epithelial cells and the required mucosal microenvironment.
To date only the assembly of immunoglobulins having light, heavy and J chain within the same cell has been reported. See Carayannopoulos et al. Proc. Nat. Acad. Sci. U.S.A., 91:8348-8352 (1994). However, the assembly of an immunoglobulin having the additional protein component, secretory component, within a single cell has not been described.
The present invention discloses a novel method for the assembly of these complex molecules. Rather than assemble the tetrameric complex at the epithelial cell surface by the interaction of a membrane bound polyimmunoglobulin receptor with immunoglobulin, we have assembled secretory immunoglobulin composed of alpha, J, and kappa immunoglobulin chains associated with a protection protein derived from pIgR. This invention produces transgenic plants that assemble secretory immunoglobulins with great efficiency. The present invention makes passive immunotherapy economically feasible.

SUMMARY OF THE INVENTION

The present invention contemplates a new type of immunoglobulin molecule. Immunoglobulins of the present invention contain a protection protein in association with an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain. In other embodiments, the immunoglobulin of the present invention further comprise an immunoglobulin derived light chain having at least a portion of an antigen binding domain associated with the immunoglobulin derived heavy chain.
The protection proteins of the present invention give the immunoglobulins containing these protein useful properties including resistance to chemical and enzymatic degradation and resistance to denaturation. These protection proteins enhanced the resistance of the immunoglobulins to environmental conditions.
The protection proteins of the proteins of the present invention comprise at least a segment of amino acid residues 1 to 606 of native polyimmunoglobulin receptor (pIgR) of any species. Other useful protection proteins include protection proteins that contain portions of the pIgR molecule. For example, the protection protein may comprise all or part of: amino acids 1-118 (domain I of rabbit pIgR), amino acids 1 to 223 (domains I and II of rabbit pIgR); amino acids 1 to 332 (domains I, II, III of rabbit pIgR); amino acids 1 to 441 (domains I, II, III, and IV rabbit of pIgR); amino acids 1 to 552 (domains I, II, III, IV and V of rabbit pIgR); and amino acids 1 to 606 or 1 to 627 of pIgR. Additional amino acids, derived either from the pIgR sequence 653-755, or from other sources, may be included so long as they do not constitute a functional transmembrane spanning segment.
In other preferred embodiments, the immunoglobulins of the present invention have a protection protein which has a first amino acid sequence which substantially corresponds to at least a portion of the amino acid residues 1 to 606 or 1 to 627 of the rabbit polyimmunoglobulin receptor and has a second amino acid residue sequence contiguous with said first amino acid sequence, wherein said second amino acid residue sequence does not have an amino acid residue sequence corresponding to the transmembrane segment of the rabbit polyimmunoglobulin receptor.
In more preferred embodiments, the second amino acid residue sequence has at least a portion of an amino acid sequence which corresponds to amino acid residues 655 to 755 of a polyimmunoglobulin receptor. In other preferred embodiments, the second amino acid residue is at least a portion of one or more of the following: an intracellular domain of a polyimmunoglobulin molecule, a domain of a member of the immunoglobulin gene superfamily, an enzyme, a toxin, or a linker.
The present invention contemplates protection proteins which do not have an amino acid residue corresponding to the transmembrane segment of rabbit polyimmunoglobulin receptor but may have amino acid residues corresponding to the intracellular domain of the rabbit polyimmunoglobulin receptor and this are deletion mutants of the receptor.
The present invention also contemplates immunoglobulins containing protection proteins which have an amino acid sequence which does not contain amino acid residues of a polyimmunoglobulin receptor from a species which are analogous to amino acid residues 288 to 755 of the rabbit immunoglobulin receptor, but does contain at least a portion of the amino acid residues or the domains from a polyimmunoglobulin receptor of a species which are analogous to one or more of these amino acid segments: Amino acids corresponding to amino acid residues 20-45 of the rabbit polyimmunoglobulin receptor; amino acids corresponding to or analogous to amino acid residues 1 to 120 of the rabbit polyimmunoglobulin receptor: amino acids corresponding to or analogous to amino acid residues numbers 120-230 of the rabbit immunoglobulin receptor; amino acids corresponding to or analogous to amino acid residues numbers 230-340 of the rabbit polyimmunoglobulin receptor; amino acids corresponding to or analogous to amino acid residues 340-456 of the rabbit polyimmunoglobulin receptor; amino acids corresponding to or analogous to amino acid residues numbers 450-550 to 570 of the rabbit polyimmunoglobulin receptors; amino acids corresponding to or analogous to amino acid residues 550 to 570-606 to 627 of the rabbit polyimmunoglobulin receptor.
The protection proteins of the present invention may be derived from many species and include protection proteins derived from mammals, rodents, humans, bovine, porcine, ovine, fowl, caprine, mouse, rat, guinea pig, chicken or other bird and rabbit.
In preferred embodiments, the immunoglobulins of the present invention contain two or four immunoglobulin derived heavy chains having at least a portion of an antigen binding domain associated with the protection protein and two or four immunoglobulin derived light chains having at least a portion of an antigen binding domain bound to the each of the immunoglobulin derived heavy chains.
In other preferred embodiments, the immunoglobulins of the present invention further comprise immunoglobulin J chain bound to at least one of the immunoglobulin derived heavy chains. In preferred embodiments, the component parts of the immunoglobulins of the present invention are bound together by hydrogen bonds, disulfide bonds, covalent bonds, ionic interactions or combinations of said bonds. In other preferred embodiments, the immunoglobulin of the present invention contain protection proteins and/or immunoglobulin derived heavy, light or J chains that are free from N-linked and/or O-linked oligosaccharides.
The immunoglobulins of the present invention may be used as therapeutic immunoglobulins against, for example, mucosal pathogen antigens. In preferred embodiments, the immunoglobulins of the present invention are capable of preventing dental caries by binding to an antigen from S. mutans serotypes c, e and f; and S. sobrinus stereotype d and g, using older nomenclature S. mutans a, c, d, e, f, g and h.
The present invention also contemplates a eukaryotic cell, including a plant cell, containing an immunoglobulin of the present invention. Eukaryotic cells, including plant cells, containing a nucleotide sequence encoding a protection protein and a nucleotide sequence encoding an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain is also contemplated. Eukaryotic cells, including plant cells, that additionally contain a nucleotide sequence encoding an immunoglobulin derived light chain having at least a portion of an antigen binding domain is also contemplated. In preferred embodiments, the eukaryotic cells, including plant cells, of the present invention contain nucleotide sequences that encode immunoglobulins that have an antigen binding domain is capable of binding an antigen from S. mutans serotypes a, c, d, e, f, and g, h (S. mutans serotypes c, e and f and S. sobrinus serotypes d and g under new nomenclature. The nucleotide sequences include RNA and appropriate DNA molecules arranged for expression.
In preferred embodiments, the plant cells of the present invention are part of a plant such as a whole plant. The present invention contemplates the use of all types of plants, both dicotyledonous and monocotyledonous including alfalfa, and tobacco.
The present invention also contemplates compositions comprising an immunoglobulin of the present invention and plant macromolecules derived from one of the plants useful in practicing the present invention. Particularly contemplated are compositions containing ribulose bisphosphate carboxylase, light harvesting complex, pigments, secondary metabolites or chlorophyll and an immunoglobulin of the present invention. Preferred compositions have an immunoglobulin concentration of between 0.001% and 99.9% mass excluding water. In more preferred embodiments, the immunoglobulin concentrations present in the composition is between 0.1% and 99%. Other preferred compositions have plant macromolecules present in a concentration of between 1% and 99% mass excluding water.
The present invention also contemplates methods for making an immunoglobulin of the present invention comprising introducing into a plant cell an expression vector having a nucleotide sequence encoding a protection protein operably linked to a transcriptional promoter; and introducing into the same plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain, operably linked to a transcriptional promoter. Other methods that further include the step of introducing into the same plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin derived light chain having at least a portion of an antigen binding domain, operably linked to a transcriptional promoter. Other preferred methods include also introducing into a plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin J chain operably linked to a transcriptional promoter.
The present invention also contemplates methods for producing assembled immunoglobulins having heavy, light and J chains and a protection protein by introducing into a eukaryotic cell nucleotide sequences operatively linked for expression to encode an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain, an immunoglobulin light chain having at least a portion of an antigen binding domain, and immunoglobulin J chain, and a protection protein. The method further comprises maintaining the eukaryotic cell under conditions allowing the production and assembly of the immunoglobulin derived heavy and light chains together with the immunoglobulin J chain and the protection protein to form an immunoglobulin containing a protection protein.
The present invention also contemplates methods of making an immunoglobulin resistant to various environmental conditions (more stable) and harsh conditions by operatively linking a nucleotide sequence encoding at least a portion of a desirable antigen binding domain derived from an immunoglobulin heavy chain to a nucleotide sequence encoding at least one domain derived from an immunoglobulin μ or α (IgM or IgA) heavy chain (or other immunoglobulin having increased stability in the environment) to form a nucleotide sequence encoding a chimeric immunoglobulin heavy chain and expressing that nucleotide sequence in a eukaryotic which also contains at least one molecule from the following list: a protection protein, an immunoglobulin derived light chain having at least a portion of an antigen binding domain and an immunoglobulin J chain. The method further comprises allowing the chimeric immunoglobulin heavy chain to assemble with the other molecule present in the same cell to form an immunoglobulin which is resistant to environmental conditions and more stable.
The large scale production of immunoglobulins of the present invention is contemplated by growing the plants of the present invention and extracting the immunoglobulins from those plants. In preferred embodiments, the method of producing therapeutic immunoglobulin compositions containing plant macromolecules includes the step of shearing under pressure a portion of a plant of the present invention to produce a pulp containing a therapeutic immunoglobulin and plant macromolecules in an liquid derived from the apoplast or symplast of the plant and solid plant derived material. Further processing steps are contemplated which include separating the solid plant derived material from the liquid and using a portion of the plant including a leaf, stem, root, tuber, flower, fruit, seed or entire plant. The present invention contemplates the use of a mechanical device or enzymatic method which releases liquid from the apoplast or symplast of said plant followed optionally by separating using centrifugation, settling, flocculation or filtration.
The present invention contemplates immunoglobulins that are chimeric and thus they contain immunoglobulin domains derived from different immunoglobulin molecules. Particularly preferred are immunoglobulins containing domains from IgG, IgM and IgA.
The present invention contemplates immunoglobulins where the immunoglobulin derived heavy chain is comprised of immunoglobulin domains from two different isotopes of immunoglobulin. In preferred embodiments, the immunoglobulin domains used include at least the C_H1, C_H2, or C_H3 domain of mouse IgG, IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, or IgD or the Cvar domain. In other preferred embodiments, the immunoglobulin heavy chain is comprised of at least the Cμ1, Cμ2, Cμ3 or Cμ4 domain of mouse IgM.
The present invention also contemplates immunoglobulin derived heavy chains made up of immunoglobulin domains include at least the C_H1, C_H2, or C_H3 domain of a human IgG, IgG1, IgG2, IgG3, IgG4, IgA1, IgA2, or IgD; or least the Cμ1, Cμ2, Cμ3 or Cμ4 domain of human IgM; or the Cvar domain. The use of immunoglobulin domains derived from mammals, animals or rodents including any IgG isotype, any IgA isotype, IgE, IgM or IgD is contemplated.
The present invention also contemplates tetratransgenic organisms which are comprised of cells containing four different transgenes each encoding a different polypeptide of a multipeptide molecule wherein at least one of those peptides is associated together to form a multipeptide molecule. The transgenic organisms contemplated by the present invention include transgenic organisms which contain as one of the four transgenes present a transgene encoding a protection protein. The protection protein present in the transgenic organism's cells is able to assemble together with immunoglobulin heavy chains when present to form immunoglobulins which contain the protection protein.
In preferred transgenic organisms, the cells of the organism express four transgenes which encode an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain, an immunoglobulin derived light chain having at least a portion of an antigen binding domain, an immunoglobulin J chain, and a protection protein. In other preferred transgenic organisms, the cells contain a transgene which encodes a chimeric immunoglobulin heavy chain, an immunoglobulin heavy chain derived form an IgA heavy chain, an immunoglobulin derived from an IgM heavy chain or an immunoglobulin derived from some other isotype of heavy chain.
In the most preferred embodiment, the transgenic organisms of the present invention are a plant. Various types and species of plants are contemplated by the present invention. In addition, the present invention also contemplates mammals which are transgenic organisms containing the various molecules of the present invention. Mammalian transgenic organisms are contemplated by the present invention and include mammalian transgenic organisms which contain four transgenes encoding different polypeptides.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings will first briefly be described.

FIG. 1 illustrates synthetic oligonucleotides J1-J5 (restriction enzyme sites are underlined) that were used to amplify DNA fragments for Guy's 13 and alpha chain domains in the construction of hybrid IgG/A heavy chains. The relative positions of the areas encoded by each oligonucleotide are shown diagrammatically The resulting recombinant heavy chains produced by combining various DNA fragments expressed in plants are also shown.

DETAILED DESCRIPTION OF THE INVENTION

A. Definitions

Dicotyledon (dicot): A flowering plant whose embryos have two seed halves or cotyledons. Examples of dicots are: tobacco; tomato; the legumes including alfalfa; oaks; maples; roses; mints; squashes; daisies; walnuts; cacti; violets; and buttercups.
Monocotyledon (monocot): A flowering plant whose embryos have one cotyledon or seed leaf. Examples of monocots are: lilies; grasses; corn; grains, including oats, wheat and barley; orchids; irises; onions and palms.
Lower plant: Any non-flowering plant including ferns, gymnosperms, conifers, horsetails, club mosses, liver warts, hornworts, mosses, red algaes, brown algaes, gametophytes, sporophytes of pteridophytes, and green algaes.
Eukaryotic hybrid vector: A DNA by means of which a DNA coding for a polypeptide (insert) can be introduced into a eukaryotic cell.
Extrachromosomal ribosomal DNA (rDNA): A DNA found in unicellular eukaryotes outside the chromosomes, carrying one or more genes coding for ribosomal RNA and replicating autonomously (independent of the replication of the chromosomes).
Palindromic DNA: A DNA sequence with one or more centers of symmetry.
DNA: Deoxyribonucleic acid.
T-DNA: A segment of transferred DNA.
rDNA: Ribosomal DNA.
RNA: Ribonucleic acid.
rRNA: Ribosomal RNA.
Ti-plasmid: Tumor-inducing plasmid.
Ti-DNA: A segment of DNA from Ti-plasmid.
Insert: A DNA sequence foreign to the rDNA, consisting of a structural gene and optionally additional DNA sequences.
Structural gene: A gene coding for a polypeptide and being equipped with a suitable promoter, termination sequence and optionally other regulatory DNA sequences, and having a correct reading frame.
Signal Sequence: A DNA sequence coding for an amino acid sequence attached to the polypeptide which binds the polypeptide to the endoplasmic reticulum and is essential for protein secretion.
(Selective) Genetic marker: A DNA sequence coding for a phenotypical trait by means of which transformed cells can be selected from untransformed cells.
Promoter: A recognition site on a DNA sequence or group of DNA sequences that provide an expression control element for a gene and to which RNA polymerase specifically binds and initiates RNA synthesis (transcription) of that gene.
Inducible promoter: A promoter where the rate of RNA polymerase binding and initiation is modulated by external stimuli. Such stimuli include light, heat, anaerobic stress, alteration in nutrient conditions, presence or absence of a metabolite, presence of a ligand, microbial attack, wounding and the like.
Viral promoter: A promoter with a DNA sequence substantially similar to the promoter found at the 5′ end of a viral gene. A typical viral promoter is found at the 5′ end of the gene coding for the p21 protein of MMTV described by Huang et al., Cell, 27:245 (1981). Other examples include the promoters found in the 35S transcript of the cauliflower mosaic virus as described by Benfey et al., Science, 250:959 (1990).
Synthetic promoter: A promoter that was chemically synthesized rather than biologically derived. Usually synthetic promoters incorporate sequence changes that optimize the efficiency of RNA polymerase initiation.
Constitutive promoter: A promoter where the rate of RNA polymerase binding and initiation is approximately constant and relatively independent of external stimuli. Examples of constitutive promoters include the cauliflower mosaic virus 35S and 19S promoters described by Poszkowski et al., EMBO J., 3:2719 (1989) and Odell et al., Nature, 313:810 (1985).
Regulated promoter: A promoter where the rate of RNA polymerase binding and initiation is modulated at a specific time during development, or in a specific structure of an organism or both of these types of modulation. Examples of regulated promoters are given in Chua et al., Science, 244:174-181 (1989).
Single-chain antigen-binding protein: A polypeptide composed of an immunoglobulin light-chain variable region amino acid sequence (V_L) tethered to an immunoglobulin heavy-chain variable region amino acid sequence (V_H) by a peptide that links the carboxyl terminus of the V_Lsequence to the amino terminus of the V_Hsequence. Generally any combination of the heavy chain and light chain antigen binding domains into the same polypeptide using a linker polypeptide to allow the binding domains to assume a useful conformation. Such combinations include V_H-Linker-V_L, V_H-Linear-Light chain, or V_L-Linear-Fd.
Single-chain antigen-binding protein-coding gene: A recombinant gene coding for a single-chain antigen-binding protein.
Polypeptide and peptide: A linear series of amino acid residues connected one to the other by peptide bonds between the alpha-amino and carboxy groups of adjacent residues.
Protein: A linear series of greater than about 50 amino acid residues connected one to the other as in a polypeptide.
Immunoglobulin product: A polypeptide, protein or protein containing at least the immunologically active portion of an immunoglobulin heavy chain and is thus capable of specifically combining with an antigen. Exemplary immunoglobulin products are an immunoglobulin heavy chain, immunoglobulin molecules, substantially intact immunoglobulin molecules, any portion of an immunoglobulin that contains the paratope, including those portions known in the art as Fab fragments, Fab′ fragment, F(ab′)₂fragment and Fv fragment.
Immunoglobulin molecule: A protein containing the immunologically active portions of an immunoglobulin heavy chain and immunoglobulin light chain covalently coupled together and capable of specifically combining with antigen.
Immunoglobulin derived heavy chain: A polypeptide that contains at least a portion of the antigen binding domain of an immunoglobulin and at least a portion of a variable region of an immunoglobulin heavy chain or at least a portion of a constant region of an immunoglobulin heavy chain. Thus, the immunoglobulin derived heavy chain has significant regions of amino acid sequence homology with a member of the immunoglobulin gene superfamily. For example, the heavy chain in an Fab fragment is an immunoglobulin derived heavy chain.
Immunoglobulin derived light chain: A polypeptide that contains at least a portion of the antigen binding domain of an immunoglobulin and at least a portion of the variable region or at least a portion of a constant region of an immunoglobulin light chain. Thus, the immunoglobulin derived light chain has significant regions of amino acid homology with a member of the immunoglobulin gene superfamily.
Antigen binding domain: The portion of an immunoglobulin polypeptide that specifically binds to the antigen. This antigen is typically bound by antigen binding domains of the immunoglobulin heavy and light chain. However, antigen binding domains may be present on a single polypeptide.
J chain: Is a polypeptide that is involved in the polymerization of immunoglobulins and transport of polymerized immunoglobulins through epithelial cells. See, The Immunoglobulin Helper: The J Chain in Immunoglobulin Genes, at pg. 345, Academic Press (1989). J chain is found in petameric IgM and dimeric IgA and typically attached via disulphide bonds. J chain has been studied in both mouse and human.
Fab fragment: A protein consisting of the portion of an immunoglobulin molecule containing the immunologically active portions of an immunoglobulin heavy chain and an immunoglobulin light chain covalently coupled together and capable of specifically combining with antigen. Fab fragments are typically prepared by proteolytic digestion of substantially intact immunoglobulin molecules with papain using methods that are well known in the art. However an Fab fragment may also be prepared by expressing in a suitable host cell the desired portions of immunoglobulin heavy chain and immunoglobulin light chain using methods well known in the art.
F_vfragment: A protein consisting of the immunologically active portions of an immunoglobulin heavy chain variable region and an immunoglobulin light chain variable region covalently coupled together and capable of specifically combining with antigen. F_vfragments are typically prepared by expressing in suitable host cell the desired portions of immunoglobulin heavy chain variable region and immunoglobulin light chain variable region using methods well known in the art.
Asexual propagation: Producing progeny by regenerating an entire plant from leaf cuttings, stem cuttings, root cuttings, single plant cells (protoplasts) or callus.
Self-pollination: The transfer of pollen from male flower parts to female flower parts on the same plant. This process typically produces seed.
Cross-pollination: The transfer of pollen from the male flower parts of one plant to the female flower parts of another plant. This process typically produces seed from which viable progeny can be grown.
Epitope: A portion of a molecule that is specifically recognized by an immunoglobulin product. It is also referred to as the determinant or antigenic determinant.
Chimeric immunoglobulin heavy chain: An immunoglobulin derived heavy chain having at least a portion of its amino acid sequence derived from an immunoglobulin heavy chain of a different isotype or subtype or some other peptide, polypeptide or protein. Typically, a chimeric immunoglobulin heavy chain has its amino acid residue sequence derived from at least two different isotypes or subtypes of immunoglobulin heavy chain.
Transgene: A gene that has been introduced into the germ line of an animal. The gene may be introduced into the animal at an early developmental stage. However, the gene could be introduced into the cells of an animal at a later stage by, for example, a retroviral vector.
Multiple molecule: A molecule comprised of more than one peptide or polypeptide associated together by any means including chemical bonds.

B. Immunoglobulins Containing Protection Proteins

The present invention provides novel methods for producing immunoglobulin molecules containing protection proteins. The immunoglobulins contain a protection protein in association with an immunoglobulin derived heavy chain that has at least a portion of an antigen binding domain.
The protection proteins of the present invention have an amino acid sequence substantially corresponding to or analogous to at least a portion of residues 1 to 627 of the amino acid residue sequence of the rabbit polyimmunoglobulin receptor and is derived from a precursor protein that does not contain the amino acid residue sequence greater than amino acid residue 627 or analogous to amino acid residue 627 of the rabbit polyimmunoglobulin receptor. The nucleotide sequence and the amino acid sequence of the rabbit polyimmunoglobulin receptor are now and have been described by the Mostov et al., Nature, 308:37 (1984) and EMBL/Gene Bank K01291. The nucleotide sequence of the polyimmunoglobulin receptor is SEQ ID NO. 1 and the corresponding amino acid residue sequence is SEQ ID NO. 2.
The polyimmunoglobulin receptors from any species may be used as a protection protein and these protection proteins do not contain and are derived from a precursor protein that does not contain amino acids having numbers greater than the amino acid number analogous to amino acids 1-627 of the rabbit immunoglobulin sequence. In preferred embodiments, the protection protein is derived from any species and precursor protein that contains amino acids analogous to at least a portion of amino acids 1-606 of the rabbit polyimmunoglobulin receptor and does not contain amino acid residues analogous to residues 607-755 of the rabbit polyimmunoglobulin receptor.
The human polyimmunoglobulin receptor sequence has been determined and reported by Krajci et al., Eur. J. Immunol., 22:2309-2315 (1992) and Krajci et al., Biochem. Biophys. Res. Comm., 158:783-789 (1989) and EMBL/Gene Bank Accession No. X73079. The nucleotide sequence of the human polyimmunoglobulin receptor is SEQ ID NO. 3 and the corresponding amino acid residue sequence is. SEQ ID NO. 4. The human polyimmunoglobulin receptor shows extensive sequence homology and has an analogous domain structure to that of the rabbit polyimmunoglobulin receptor. See, Kraehenbuhl et al., Trends in Cell Biol., 2:170 (1992). The portions of the human polyimmunoglobulin receptor which are analogous to the domains and/or amino acid residues sequence of the rabbit polyimmunoglobulin receptor are shown in Table 1.
The rat polyimmunoglobulin receptor sequence has been determined and reported by Banting et al., FEBS Lett., 254:177-183 (1989) and EMBL/Gene Bank Accession No. X15741. The nucleotide of the rat polyimmunoglobulin receptor nucleotide sequence is SEQ ID NO. 9 and the corresponding amino acid residue sequence is SEQ ID NO 10. The rat polyimmunoglobulin receptor shows extensive sequence homology and has an analogous domain structure to that of the rabbit and human polyimmunoglobulin receptor. See, Kraehenbuhl et al., T. Cell Biol., 2:170 (1992). The portions of the rat polyimmunoglobulin receptor which are analogous to the domains and/or amino acid residue sequence of the rabbit polyimmunoglobulin receptor are shown in Table 1.
The bovine polyimmunoglobulin receptor sequence has been determined and reported in EMBL/Gene Bank Accession No. X81371. The bovine polyimmunoglobulin receptor nucleotide sequence is SEQ ID NO. 5 and the corresponding amino acid residue sequence is SEQ ID NO. 6. The bovine polyimmunoglobulin receptor shows extensive sequence homology and has an analogous domain structure to that of the rabbit and human polyimmunoglobulin receptor. The portions of the bovine polyimmunoglobulin receptor which are analogous to the domains and/or amino acid residues sequence of the rabbit polyimmunoglobulin receptor are shown in Table 1.
The mouse polyimmunoglobulin receptor sequence has been determined and reported by Piskurich et al., J. Immunol., 150:38 (1993) and EMBL/Gene Bank U06431. The mouse polyimmunoglobulin receptor nucleotide is SEQ ID NO. 7 and the corresponding amino acid residue sequence is SEQ ID NO. 8. The mouse polyimmunoglobulin receptor shows extensive sequence homology and has an analogous domain structure to that of the rabbit and human polyimmunoglobulin receptor. The portions of the mouse polyimmunoglobulin receptor which are analogous to the domains and/or amino acid residue sequence of the rabbit polyimmunoglobulin receptor are shown in Table 1.
In addition to the above-identified nucleic acid and corresponding amino acid residue sequences of the polyimmunoglobulin receptor from a variety of species, the present invention contemplates the use of a portion of a polyimmunoglobulin receptor from any species. The conserved domain structure of the polyimmunoglobulin receptor between species allows the selection of analogous amino acid residue sequences within each polyimmunoglobulin receptor from different species. The present invention contemplates the use of such analogous amino acid residue sequences from any polyimmunoglobulin receptor. The analogous sequences from several polyimmunoglobulin receptor amino acid sequences is as shown in Table 1.

TABLE 1

Analogous Regions of the Amino Acid Residue Sequence
of The Polyimmunoglobulin Receptor of Several
Species. The nucleotide sequence coordinates
approximately define the boundaries of the domains of
molecules.

Rabbit	Bovine	Human	Rat	Mouse

(SEQ ID	(SEQ ID	(SEQ ID	(SEQ ID	(SEQ ID

The protection proteins of the present invention may contain substantially less than the entire amino acid residue sequence of the polyimmunoglobulin receptor. In preferred embodiments the protection protein contains at least a portion of the amino acid residues 1 to 606 of the native polyimmunoglobulin receptor of rabbit. Unlike the native polyimmunoglobulin receptor, the protection proteins of the present invention are derived from precursor proteins that do not contain the entire amino acid residue sequence greater than the amino acid residue 627 derived from the native polyimmunoglobulin receptor and thus may contain more amino acids or fewer amino acids than secretory components. In preferred embodiments, the protection proteins of the present invention do not contain the entire amino acid residue sequence greater than amino acid residue 606 of the native polyimmunoglobulin receptor of rabbit. The present invention contemplates using only portions of the native polyimmunoglobulin receptor sequence as a protection protein. In other embodiments, it is contemplated that the protection protein may end at any amino acid between amino acid residue 606 to 627, including every amino acid position between 606 and 627, such as 607, 608, 609, 610, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626.
In preferred embodiments, a protection protein of the present invention has an amino acid sequence which corresponds to one or more of the following amino acid segments:
1) amino acids (AA) corresponding to AA 21-43 of domain I of the rabbit polyimmunoglobulin receptor;
2) amino acids (AA) corresponding to AA 1-118 of domain I of the rabbit polyimmunoglobulin receptor;
3) amino acids (AA) corresponding to AA 119-223 of domain II of the rabbit polyimmunoglobulin receptor;
4) amino acids (AA) corresponding to AA 224-332 of domain III of the rabbit polyimmunoglobulin receptor;
5) amino acids (AA) corresponding to AA 333-441 of domain IV of the rabbit polyimmunoglobulin receptor;
6) amino acids (AA) corresponding to AA 442-552 of domain V of the rabbit polyimmunoglobulin receptor;
7) amino acids (AA) corresponding to AA of 553 to 606 or 553 to 627 of domain VI of the rabbit polyimmunoglobulin receptor; and does not contain amino acid residues corresponding to AA residues 607 to 755 or 628 to 755 of the rabbit polyimmunoglobulin receptor.
It should be noted the exact boundary of a domain may vary within approximately 20 amino acids. However, the domain structure and boundaries will be understood by one skilled in the art.
In addition, the present invention contemplates protection protein ending at the following amino acid residues of the rabbit polyimmunoglobulin receptor or at an amino acid residue which corresponds to the following residues but is in the polyimmunoglobulin receptor of another species: 580-605.
In other preferred embodiments, a protection protein has an amino acid sequence which corresponds to the amino acid sequence of a polyimmunoglobulin receptor for a particular species and which is analogous to the following amino acid segments:
i) amino acids (AA) corresponding to AA 21-43 of domain I of the rabbit polyimmunoglobulin receptor;
2) amino acids (AA) corresponding to AA 1-118 of domain I of the rabbit polyimmunoglobulin receptor;
3) amino acids (AA) corresponding to AA 119-223 of domain II of the rabbit polyimmunoglobulin receptor;
4) amino acids (AA) corresponding to AA 224-332 of domain III of the rabbit polyimmunoglobulin receptor;
5) amino acids (AA) corresponding to AA 333-441 of domain IV of the rabbit polyimmunoglobulin receptor;
6) amino acids (AA) corresponding to AA 442-552 of domain V of the rabbit polyimmunoglobulin receptor;
7) amino acids (AA) corresponding to AA of 553-606 or 553-627 of domain VI of the rabbit polyimmunoglobulin receptor; and does not contain amino acid residues analogous to amino acid residues 607-755 or 630-755 of the rabbit polyimmunoglobulin receptor.
In other preferred embodiments, the protection protein comprises domains I, IV, V and AA 550-606 or 550-627 of domain VI of the rabbit polyimmunoglobulin receptor or the amino acid sequence from analogous domains and regions of a polyimmunoglobulin receptor from a different species.
In other embodiments, a protection protein of the present invention has an amino acid residue sequence which substantially corresponds to at least a portion of the amino acid residues from the polyimmunoglobulin receptor of a species which are analogous to amino acid residues 1-627 of the rabbit polyimmunoglobulin receptor. This portion of the amino acid sequence would correspond to at least a portion of the extracellular domains of the receptor of that species.
In preferred embodiments, a protection protein of the present invention has an amino acid sequence which substantially corresponds to at least a portion of the amino acid residues from the polyimmunoglobulin receptor of a species which are analogous to amino acid residues 1-606 of the rabbit polyimmunoglobulin receptor.
In other preferred embodiments, a protection protein of the present invention has an amino acid residue sequence which substantially corresponds to or is analogous to (if from a species other than rabbit) at least a portion of the following amino acid residue sequences:
1) amino acids (AA) corresponding to AA 21-43 of domain I of the rabbit polyimmunoglobulin receptor;
2) amino acids (AA) corresponding to AA 1-118 to of domain I of the rabbit polyimmunoglobulin receptor;
3) amino acids (AA) corresponding to AA 119-223 of domain II of the rabbit polyimmunoglobulin receptor;
4) amino acids (AA) corresponding to AA 224-332 of domain III of the rabbit polyimmunoglobulin receptor;
5) amino acids (AA) corresponding to AA 333-441 of domain IV of the rabbit polyimmunoglobulin receptor;
6) amino acids (AA) corresponding to AA 442-552 of domain V of the rabbit polyimmunoglobulin receptor;
7) amino acids (AA) corresponding to AA of 553-606 or 553-627 of domain VI of the rabbit polyimmunoglobulin receptor; and does not contain amino acid residues corresponding to AA 628 to 755 of the rabbit polyimmunoglobulin receptor.
In other preferred embodiments, the immunoglobulins of the present invention have a protection protein which has a first amino acid sequence which substantially corresponds to at least a portion of the amino acid residues 1 to 606 or 1 to 627 of the rabbit polyimmunoglobulin receptor and has a second amino acid residue sequence contiguous with said first amino acid sequence, wherein said second amino acid residue sequence does not have an amino acid residue sequence corresponding to the transmembrane segment of the rabbit polyimmunoglobulin receptor.
In more preferred embodiments, the second amino acid residue sequence has at least a portion of an amino acid sequence which corresponds to amino acid residues 655 to 755 of a polyimmunoglobulin receptor. In other preferred embodiments, the second amino acid residue is at least a portion of one or more of the following: an intracellular domain of a polyimmunoglobulin molecule, a domain of a member of the immunoglobulin gene superfamily, an enzyme, a toxin, or a linker.
The present invention contemplates protection proteins which do not have an amino acid residue corresponding to the transmembrane segment of rabbit polyimmunoglobulin receptor but may have amino acid residues corresponding to the intracellular domain of the rabbit polyimmunoglobulin receptor and this are deletion mutants of the receptor.
In other embodiments, protection proteins of the present invention have an amino acid sequence which substantially corresponds to at least one of the extracellular domains of polyimmunoglobulin receptor of a particular species. The protection protein may have an amino acid sequence of which a segment of that amino acid sequence which substantially corresponds to an extracellular domain of the polyimmunoglobulin receptor of one species, and a different segment of that amino acid sequence may be from a second species and substantially correspond to an extracellular domain from a different species. This invention contemplates embodiments in which a protection protein has an amino acid sequence which has one amino acid sequence segment which corresponds to the amino acid sequence of the polyimmunoglobulin receptor from one species and has a second amino acid sequence within the same domain which corresponds to the amino acid and sequence of the polyimmunoglobulin receptor of a different species.
Thus, the protection protein may have individual domains or portions of a particular domain that are comprised of amino acid sequences which correspond to the polyimmunoglobulin receptor from different species.
Other embodiments are contemplated in which protection protein has portions of its amino acid sequence derived from a molecule which is a member of the immunoglobulin superfamily. See, Williams and Barclay, “The Immunoglobulin Superfamily.” In Immunoglobulin Genes, p. 361, Academic Press (Honjo Alt and Rabbits Eds. 1989). These derived portions may include amino acid sequences encoding peptides, domains or multiple domains from an immunoglobulin superfamily molecule.
The present invention also contemplates a nucleotide sequence encoding a protection protein which has a first nucleotide sequence encoding at least a portion of amino acids 1-606 or 1-627 of the rabbit polyimmunoglobulin receptor nucleotide sequence and which does not have a nucleotide sequence which encodes a functional transmembrane segment 3′ of the first nucleotide sequence. Further preferred embodiments include a second nucleotide sequence located 3′ of the first nucleotide sequence which encodes the amino acids 1-606 or 1-627 of the rabbit polyimmunoglobulin receptor sequence. This second nucleotide sequence may encode a variety of molecules including portions of the intracellular domain of rabbit polyimmunoglobulin receptor or another polyimmunoglobulin receptor or a portion of an immunoglobulin superfamily molecule. In addition, embodiments are contemplated in which this second nucleotide sequence encodes various effector molecules, enzymes, toxins and the like. Preferred embodiments include a second nucleotide sequence which encodes amino acid residues which correspond to amino acid residues 655 to 775 of the rabbit polyimmunoglobulin receptor or polyimmunoglobulin receptor from another species.
The present invention also contemplates expression vectors containing a nucleotide sequence encoding a protection protein which has been operatively linked to for expression. These expression vectors place the nucleotide sequence to be expressed in a particular cell 3′ of a promoter sequence which causes the nucleotide sequence to be transcribed and expressed. The expression vector may also contain various enhancer sequences which improve the efficiency of this transcription. In addition, such sequences as terminators, polydenylation (poly A) sites and other 3′ end processing signals may be included to enhance the amount of nucleotide sequence transcribed within a particular cell.
In preferred embodiments, the protection protein is part of an immunoglobulin that is in association with an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain. Immunoglobulin derived heavy chains containing at least a portion of an antigen binding domain are well known in the art and have been described, for example, by Huse et al., Science, 246:1275 (1989), and by Lerner and Sorge, PCT Application WO 90/14430, published Nov. 29, 1990. The disclosure of these documents are hereby incorporated by reference.
In other embodiments, the immunoglobulins of the present invention contain a protection protein and immunoglobulin derived heavy chain and immunoglobulin derived light chain that contain at least a portion of an antigen binding site in association with the immunoglobulin derived heavy chain. Immunoglobulin light chains having at least a portion of an antigen binding domain are well known in the art and are described in available sources. See, for example, Early and Hood, Genetic Engineering, Setlow & Hollaender, (eds.), Vol. 3, Plenum Publishing Corp., New York (1981), pages 157-188; and Kabat et al., Sequences of Immunologic Interest, National Institutes of Health, Bethesda, Md. (1987). The disclosures of all references cited herein are hereby incorporated by reference.
The immunoglobulin components of the complex (alpha, J, kappa or lambda) can contain all or part of the full length polypeptide. Parts of these chains may be used to substitute for the whole chain. For instance, the entire immunoglobulin alpha heavy chain may be replaced by the variable region and only a portion of the alpha constant region sufficient to enable assembly with the other components. Likewise, a truncated kappa or lambda chain, containing only a small section of constant region can replace the full length kappa or lambda chains. The prerequisite of any complex is the ability to bind the protection protein.
In addition to truncated components, the present invention contemplates the combination of different types of immunoglobulins. For example, a heavy chain constant region comprising the C_H1 and C_H2 regions of IgG followed by the C_H2 and C_H3 regions derived from an IgA will form a stable complex containing the protection protein. This is specifically described as an example.
The immunoglobulins containing the protection proteins of the present invention preferably contain at least a portion of an IgM or IgA heavy chain which allows that immunoglobulin heavy chain to bind to immunoglobulin J chain and thereby bind to the protection protein. It is contemplated that the immunoglobulin heavy chain of the present invention may be comprised of individual domains selected from the IgA heavy chain or the IgM heavy chain or from some other isotype of heavy chain. It is also contemplated that an immunoglobulin domain derived from an immunoglobulin heavy chain other than IgA or IgM may be molecularly engineered to bind immunoglobulin J chain and thus may be used to produce immunoglobulins of the present invention.
One skilled in the art will understand that immunoglobulins consist of domains which are approximately 100-110 amino acid residues. These various domains are well known in the art and have known boundaries. The removal of a single domain and its replacement with a domain of another antibody molecule is easily achieved with modern molecular biology. The domains are globular structures which are stabilized by intrachain disulfide bonds. This confers a discrete shape and makes the domains a self-contained unit that can be replaced or interchanged with other similarly shaped domains. The heavy chain constant region domains of the immunoglobulins confer various properties known as antibody effector functions on a particular molecule containing that domain. Example effector functions include complement fixation, placental transfer, binding to staphyloccal protein, binding to streptococcal protein G, binding to mononuclear cells, neutrophils or mast cells and basophils. The association of particular domains and particular immunoglobulins isotopes with these effector functions is well known and for example, described in Immunology, Roitt et al., Mosby St. Louis, Mo. (1993 3rd Ed.)
The immunoglobulins of the present invention may, in addition to the protection protein, contain immunoglobulin heavy chains, immunoglobulin light chains, or immunoglobulin J chain bound to the immunoglobulin derived heavy chains. In preferred embodiments, the immunoglobulin of the present invention comprises two or four immunoglobulin derived heavy chains, together with two or four immunoglobulin light chains and an immunoglobulin J chain bound to at least one of the immunoglobulin derived heavy chains. The immunoglobulin J chain is described and known in the art. See, for example, M. Koshland, The Immunoglobulin Helper: The J Chain, in Immunoglobulin Genes, Academic Press, London, Pg. 345, (1989) and Matsuuchi et al., Proc. Natl. Acad. Sci. U.S.A., 83:456-460 (1986). The sequence of the immunoglobulin J chain is available on various data bases in the United States.
The immunoglobulin of the present invention has a protection protein associated with at least an immunoglobulin derived heavy chain. This association may occur by hydrogen bonds, disulfide bonds, covalent bonds, ionic interactions or combinations of these various bonds. Typically, immunoglobulin molecules are held together by disulfide bonds between the immunoglobulin heavy chains and immunoglobulin light chains. The interaction of the protection protein with the immunoglobulin is by non-covalent or disulfide bonding.
The immunoglobulins of the present invention containing the protection protein, the immunoglobulin derived heavy chain and optionally an immunoglobulin derived light chain, and J chain are typically bonded together by one of the following: hydrogen bonds, disulfide bonds, covalent bonds, ionic interactions or combinations of these bonds. The present invention contemplates molecules in which the required portions of the immunoglobulin heavy, light and/or J chain have been placed into a single polypeptide and function to bind antigen and protection protein. Examples of such proteins are single-chain antigen-binding proteins.
The present invention contemplates a method of assembling a multimeric immunoglobulin comprising the steps of: introducing into an organism a DNA segment encoding all or part of an immunoglobulin J chain, and a DNA segment encoding all or part of an immunoglobulin alpha chain, and a DNA segment encoding all or part of either an immunoglobulin kappa chain or an immunoglobulin lambda chain; and introducing into the same organism a protection protein, said protection protein comprising at least a segment of the amino acid residues 1 to residue 606 of the rabbit polyimmunoglobulin receptor (pIgR) amino acid residue sequence or analogous amino acid residues from other species such that the segment is derived from a precursor protein that does not contain the amino acid residues comprising a functional membrane spanning region nor is the segment derived from a precursor protein in which the sequence of amino acid residues from the beginning of the membrane spanning region (approximately residue 630 of rabbit polyimmunoglobulin receptor) to the carboxyl end of the protein (approximately residue 755 of the rabbit polyimmunoglobulin receptor) are fully intact. In preferred embodiments the precursor protein does not contain amino acid residues greater than 606 of the rabbit polyimmunoglobulin receptor or analogous amino acid residues from other species.
As is understood by those of ordinary skill in the art, a membrane spanning region or functional transmembrane segment consists of a contiguous section of amino acid residues containing from about 20 to about 30 amino acids in which none of the residues is charged, virtually all of the residues are hydrophobic or non-polar, and the segment forms an alpha helix. A functional transmembrane segment is capable of spanning a biomembrane. Membrane spanning regions can be bounded by charged residues. An example of a membrane spanning region of pIgR is residues 630 to 653 of the polyimmunoglobulin receptor amino acid residue sequence of rabbit.
The chains that comprise the immunoglobulin containing the protection protein may be derived from precursors containing a signal sequence at the amino terminal of the protein. Each component can thereby be synthesized into an endomembrane system where assembly occurs. In addition to a signal sequence, the various components of the complex may or may not contain additional signals for N terminal glycosylation or for various other modifications which can affect the structure of the complex. In one embodiment of the invention, the signals for glycosylation (i.e. asparagine-X-serine or threonine or the signals for O-linked glycosylation) are not present or present in more or less places within the nucleotide sequence. The resulting antibody therefore would contain no carbohydrate, which may be advantageous for applications in which carbohydrates elicit an immune response.
In preferred embodiments, the immunoglobulin of the present invention contains a protection protein associated with an immunoglobulin derived heavy chain and the protection protein is free from N-linked and/or O-linked oligosaccharides. One skilled in the art will understand that a gene coding for a polypeptide having within its amino acid residue sequence the N-linked glycosylation signal asparagine-X-serine/threonine where X can be any amino acid residue except possibly proline and aspartic acid, when introduced into a plant cell would be glycosylated via oligosaccharides linked to the asparagine residue of the sequence (N-linked). See, Marshall, Ann. Rev. Biochem., 41:673 (1972) and Marshall, Biochem. Soc. Symp., 40:17 (1974) for a general review of the polypeptide sequences that function as glycosylation signals. These signals are recognized in both mammalian and in plant cells. One skilled in the art will understand that the N-linked glycosylation signal may be easily removed using common mutagenesis procedures to change the DNA sequence encoding the protection protein of the present invention. This mutagenesis typically involves the synthesis of oligonucleotide having the N-linked glycosylation signal deleted and then preparing a DNA strand with that oligonucleotide sequence incorporated into it. Such mutagenesis procedures and reagents are commercially available from many sources such as Stratagene (La Jolla, Calif.).
Assembly of the individual polypeptides that form a multi-peptide molecule (for example immunoglobulin) may be obtained by expressing in a single cell by directly introducing all the transgenes encoding the individual polypeptides into that cell either sequentially or all at once. The transgenes encoding the polypeptides may be present on individual constructs or DNA segments or may be contained in a DNA segment or construct together with one or more other transgenes.
Assembly of these components can be by cross pollination as originally described by Mendel to produce a population of segregants expressing all chains. Previous disclosures have demonstrated this to be an adequate method for the assembly and co-segregation of multimeric glycoconjugates. The disclosure of U.S. Pat. No. 5,202,422 is hereby incorporated by reference and describes these methods. In a preferred embodiment of the present invention, the antibody molecules contain a reduced number of glycans and antibody molecules with no glycans are contemplated.
The immunoglobulins of the present invention containing the protection protein, the immunoglobulin derived heavy chain and optionally an immunoglobulin derived light chain, and J chain may contain a protection protein that is free from N-linked oligosaccharides.
The immunoglobulins of the present invention that contain the protection protein are preferably therapeutic immunoglobulins that are useful in preventing a disease in an animal. In preferred embodiments, the immunoglobulins of the present invention are therapeutic immunoglobulins which are capable of binding to mucosal pathogen antigens. In other preferred embodiments, the therapeutic immunoglobulins of the present invention are capable of preventing dental caries. In the most preferred embodiment, the immunoglobulin of the present invention containing the protection protein contains an antigen binding domain that is capable of binding to an antigen from S. mutans serotypes a, c, d, e, f, g and h (S. mutans c, e and f and S. sobrinus serotypes d and g under new nomenclature). Such antigen binding domains are known in the art and include, for example, the binding domains described in U.S. Pat. No. 5,352,446, J. K-C. Ma et al., Clin. Exp. Immunol. 77:331 (1989); and J. K-C. Ma et al., Eur. J. Immunol. 24:131-138 (1994); U.S. Pat. No. 5,352,446; U.S. Pat. No. 4,594,244; and European Patent Publication 371 017 B1. The disclosures of these documents are hereby incorporated by reference. In preferred embodiments, the immunoglobulins of the present invention are part of a composition that has a therapeutic activity on either animals or humans. Examples of therapeutic immunoglobulins are numerous, however, we envision the most appropriate therapeutic effect to be prophylaxis for mucosal and enteric pathogens by direct oral administration of the composition derived from an edible plant.
Administration of the therapeutic composition can be before or after extraction from the plant or other transgenic organism. Once extracted the immunoglobulins may also be further purified by conventional techniques such as size exclusion, ion exchange, or affinity chromatography. In the preferred embodiment, the transgenic organism is an edible plant and administration of the complex is by ingestion after partial purification. Plant molecules may be co-administered with the complex.
The present invention also contemplates that the relative proportion of plant-derived molecules and animal-derived molecules can vary. Quantities of specific plant proteins, such as RuBisCo, or chlorophyll may be as little as 1% of the mass or as much as 99.9% of the mass of the extract, excluding water.
The present invention also contemplates the use of the therapeutic plant extract containing immunoglobulins having a protection protein directly without any further purification of the specific therapeutic component, e.g. the antibody. Administration may be by topical application, oral ingestion or any other method appropriate for delivering the antibody to the mucosal target pathogen. This form of administration is distinct from parenteral applications involving direct injection or commingling of the therapeutic plant extract with the blood stream.
The present invention also contemplates the use of the therapeutic plant extract containing immunoglobulins having a protection protein after manipulating the taste or texture of the extract. Appropriate quantities of gelling substances or flavorings could be added to enhance the contact of the antibody with the target pathogen in, for example, direct oral applications.
In preferred embodiments, the immunoglobulins of the present invention are used to passively immunize an animal against a preselected ligand by contacting a composition comprising an immunoglobulin containing a protection protein of the present invention that is capable of binding a preselected ligand with a mucosal surface of an animal. Passive immunization requires large amounts of antibody and for wide-spread use this antibody must be inexpensive.
Immunoglobulin molecules containing protection proteins that are capable of binding a preselected antigen can be efficiently and economically produced in plant cells. In preferred embodiments, the immunoglobulin molecule is either IgA, IgM, secretory IgM or secretory IgA or an immunoglobulin having a chimeric immunoglobulin heavy or light chain.
The immunoglobulins containing protection proteins are more resistant to proteolysis and denaturation and therefore are desirable for use in harsh environments. Contemplated harsh environments include acidic environments, protease containing environments, high temperature environments, and other harsh environments. For example, the gastrointestinal tract of an animal is a harsh environment where both proteases and acid are present. See, Kobayashi et al., Immunochemistry, 10:73 (1973).
Passive immunization of the animal using these more resistant immunoglobulins of the present invention is produced by contacting the immunoglobulin containing the protection protein with a mucosal surface of the animal. Animals have various mucosal surfaces including the lungs, the digestive tract, the nasopharyngeal cavity, the urogenital system, and the like. Typically, these mucosal surfaces contain cells that produce various secretions including saliva, lacrimal fluid, nasal fluid, tracheobronchial fluid, intestinal fluid, bile, cervical fluid, and the like.
In preferred embodiments the immunoglobulins that contain the protection protein are immunospecific for a preselected antigen. Typically, this antigen is present on a pathogen that causes a disease that is associated with the mucosal surface such as necrotizing enterocolitis, diarrheal disease, ulcers, and cancer caused by carcinogen absorption in the intestine. See e.g., McNabb and Tomasi, Ann. Revl. Microbiol. 35:477 (1981) and Lawrence et al., Science, 243:1462 (1989). Typical pathogens that cause diseases associated with a mucosal surface include both bacterial and viral pathogens, such as E. coli., S. typhimurium, V. cholera, H. pylori, and S. mutans. See also, European Patent Application 484, 148 A1, published May 6, 1992 and hereby incorporated by reference. The immunoglobulins of the present invention are capable of binding to these pathogens and preventing them from causing mucosal associated diseases.
Immunoglobulins capable of binding to S. mutans and preventing dental caries have been described in European Patent Specification 371,017 which is hereby incorporated by reference. The disclosure of U.S. Pat. No. 5,352,440 is also hereby incorporated by reference.
Therapeutic immunoglobulins of the present invention that contain protection proteins that would be effective against bacterial infection or carcinomas are contemplated. Monoclonal antibodies with therapeutic activity have been described in U.S. Pat. Nos. 4,652,448, 4,443,549 and 5,183,756 which are hereby incorporated by reference.
In preferred embodiments, the immunoglobulin of the invention are part of a composition which is contacted with the animal mucosal surface comprises plant material and an immunoglobulin of the present invention that is capable of binding a preselected ligand. The plant material present may be plant cell walls, plant organelles, plant cytoplasms, intact plant cells, viable plants, and the like. This plant cell material is present in a ratio from about 10,000 grams of plant material to about 100 nanograms of immunoglobulin to about 100 nanograms of plant material for each 10 grams of immunoglobulin present. In more preferred embodiments, the plant material is present in a ratio from about 10,000 grams of plant material for each 1 gram of immunoglobulin present to about a ratio of 100 nanograms of plant material present for each gram of immunoglobulin present. In other preferred embodiments, the plant material is present in a ratio from about 10,000 grams of plant material for each milligram of immunoglobulin present to about 1 milligram of plant material present for each 500 milligram of immunoglobulin present.
In preferred embodiments, the composition containing the immunoglobulins of the present invention is a therapeutic composition. The preparation of therapeutic compositions which contain polypeptides or proteins as active ingredients is well understood in the art. Therapeutic compositions may be liquid solutions or suspensions, solid forms suitable for solution in, or suspension in a liquid prior to ingestion may also be prepared. The therapeutic may also be emulsified. The active therapeutic ingredient is typically mixed with inorganic and/or organic carriers which are pharmaceutically acceptable and compatible with the active ingredient. The carriers are typically physiologically acceptable excipients comprising more or less inert substances when added to the therapeutic composition to confer suitable consistencies and form to the composition. Suitable carriers are for example, water, saline, dextrose, glycerol, and the like and combinations thereof. In addition, if desired the composition can contain minor amounts of auxiliary substances such as wetting or emulsifying agents and pH buffering agents which enhance the effectiveness of the active ingredient. Therapeutic compositors containing carriers that have nutritional value are also contemplated.
In embodiments in which a composition containing an immunoglobulin having a protection protein of the present invention is applied to the tooth or mouth of a mammal, any convenient method may be used. Methods for applying such a composition to the teeth are well known and utilize various materials for a variety of purposes. For example, the composition may be directly applied to the tooth by painting the surface of the tooth with that composition. Alternatively, the composition of the present invention may be included in a toothpaste, mouthwash, chewing gum, lozenge or gel that will result in it being applied to the teeth. In some formulations, it may be desirable to provide for a formulation that prolongs the contact of the composition and therefore the immunoglobulin having the protection protein with the tooth surface. Formulations for this purpose are well known and include such formulations that may be placed in various dental trays that are used to cover the tooth and other dental apparatuses that are used in adjusting various conditions with the teeth.
The exact amount of a composition that must be applied to the teeth during any particular application is not critical because such treatment may be easily repeated at a given interval. For example, compositions present in toothpaste would be applied to the teeth each time that toothpaste is used, typically twice per day. For example, the order of 10 to 100 micrograms of an immunoglobulin having a protection protein can be applied to each tooth on each occasion the composition is applied to the teeth. However, this in no way should be taken as a limitation on a range that may be applied during any particular application as applications of a composition having more or less immunoglobulin of the present invention may be used without detrimental effect. The use of much lower concentrations of an immunoglobulin of the present invention would result in, at some point, a reduction in the protection provided by such formulation.
The exact formulation for the composition of the present invention may vary and will depend on the method of application to be used and the frequency of that application. In general, it may be any formulation which has an appropriate pH and which is free of material which would render the immunoglobulin having the protection protein of the present invention ineffective. For example, the compositions of the present invention may be applied as a simple aqueous solution in which the composition is disbursed at anywhere from 0.1 to 10 milligrams of immunoglobulin per 100 microliters of that solution. Generally, such a solution would be applied during dental surgery at a rate of approximately 1 to 10 microliters of the solution per tooth.
The formulations of the compositions of the present invention which are designed to be self-administered may vary and will be formulated taking in to account the frequency of application of the particular product in which is it used.
In preferred embodiments, a composition containing an immunoglobulin of the present invention comprises an immunoglobulin molecule that is immunospecific for a pathogen antigen. Pathogens are any organism that causes a disease in another organism. Particularly preferred are immunoglobulins that are immunospecific for a mucosal pathogen antigen. A mucosal pathogen antigen is present on a pathogen that invades an organism through mucosal tissue or causes mucosal associated diseases. Mucosal pathogens include lung pathogens, nasal pathogens, intestinal pathogens, oral pathogens, and the like. For a general discussion of pathogens, including mucosal pathogens, see, Davis et al., Microbiology, 3rd ed., Harper and Row, Hagerstown, Md. (1980).
Antibodies immunospecific for a pathogen may be produced using standard monoclonal antibody production techniques. See, Antibodies: A Laboratory Manual, Harlow et al., eds., Cold Spring Harbor, N.Y. (1988). The genes coding for the light chain and heavy chain variable regions can then be isolated using the polymerase chain reaction and appropriately selected primers. See, Orlandi et al., Proc. Natl. Acad. Sci., U.S.A., 86:3833 (1989) and Huse et al., Science, 246:1275 (1989). The variable regions are then inserted into plant expression vectors, such as the expression vectors described by Hiatt et al., Nature, 342:76-78 (1989).
In a preferred embodiment, the immunoglobulin of the present invention is immunospecific for an intestinal pathogen antigen. Particularly preferred are immunoglobulins immunospecific for intestinal pathogens such as bacteria, viruses, and parasites that cause disease in the gastrointestinal tract, such as E. coli, Salmonellae, Vibrio cholerae, Salmonellae typhimurium, Shigella and H. pylori.
In other preferred embodiments, the immunoglobulin containing the protection protein present in the composition is an immunoglobulin molecule that is immunospecific for a dental pathogen such as Streptococcus mutans and the like. Particularly preferred are immunoglobulins immunospecific for a Streptococcus mutans antigen such as the immunoglobulin produced by hybridoma 15B2 (ATCC No. HB 8510); the hybridoma deposited as European Collection of Animal cells Deposit No. 86031901; and the Guy's 13 monoclonal antibody described by Ma et al., Eur. J. Immunol., 24:131 (1994) and Smith and Lehner, Oral Micro. Immunol., 4:153 (1989).
The present invention contemplates producing passive immunity in an animal, such as vertebrate. In preferred embodiments, passive immunity is produced in fish, birds, reptiles, amphibians, or insects. In other preferred embodiments passive is produced in an mammal, such as a human, a domestic animal, such as a ruminant, a cow, a pig, a horse, a dog, a cat, and the like. In particularly preferred embodiments, passive immunity is produced in an adult or child mammal.
In preferred embodiments, passive immunity is produced in an animal, such as a mammal that is weaned and therefore no longer nurses to obtain milk from its mother. Passive immunity is produced in such an animal by administering to the animal a sufficient amount of composition containing an immunoglobulin containing a protection protein immunospecific for a preselected ligand to produce a prophylactic concentration of the immunoglobulin within the animal. A prophylactic concentration of an immunoglobulin is an amount sufficient to bind to a pathogen present and prevent that pathogen from causing detectable disease within the animal. The amount of composition containing the immunoglobulin of the present invention required to produce a prophylactic concentrations will vary as is well known in the art with the size of the animal, the amount of pathogen present, the affinity of the particular immunoglobulin for the pathogen, the efficiency with which the particular immunoglobulin is delivered to its active location within the animal, and the like.

C. Eukaryotic Cells Containing Immunoglobulins Having a Protection Protein

The present invention contemplates eukaryotic cells, including plant cells, containing immunoglobulins of the present invention. The present invention also contemplates plant cells that contain nucleotide sequences encoding the various components of the immunoglobulins of the present invention. One skilled in the art will understand that the nucleotide sequences that encode the protection protein and the various immunoglobulin heavy and light chains and J chain will typically be operably linked to a promoter and present as part of an expression vector or cassette.
After the immunoglobulin heavy and light chain genes, and J chain genes are isolated, they are typically operatively linked to a transcriptional promoter in an expression vector.
Expression of the components in the organism of choice can be derived from an independently replicating plasmid, or from a permanent component of the chromosome, or from any piece of DNA which may transiently give rise to transcripts encoding the components. Organisms suitable for transformation can be either prokaryotic or eukaryotic. Introduction of the components of the complex can be by direct DNA transformation, by ballistic delivery into the organism, or mediated by another organism as for example by the action of recombinant Agrobacteria on plant cells. Expression of proteins in transgenic organisms usually requires co-introduction of an appropriate promoter element and polyadenylation signal. In one embodiment of the invention, the promoter element potentially results in the constitutive expression of the components in all of the cells of a plant. Constitutive expression occurring in most or all of the cells will ensure that precursors can occupy the same cellular endomembrane system as might be required for assembly to occur.
Expression vectors compatible with the host cells, preferably those compatible with plant cells are used to express the genes of the present invention. Typical expression vectors useful for expression of genes in plants are well known in the art and include vectors derived from the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens described by Rogers et al., Meth. in Enzymol., 153:253-277 (1987). However, several other expression vector systems are known to function in plants. See for example, Verma et al., PCT Publication No. WO87/00551; and Cocking and Davey, Science, 236:1259-1262 (1987).
The expression vectors described above contain expression control elements including the promoter. The genes to be expressed are operatively linked to the expression vector to allow the promoter sequence to direct RNA polymerase binding and synthesis of the desired polypeptide coding gene. Useful in expressing the genes are promoters which are inducible, viral, synthetic, constitutive, and regulated. The choice of which expression vector and ultimately to which promoter a nucleotide sequence encoding part of the immunoglobulin of the present invention is operatively linked depends directly, as is well known in the art, on the functional properties desired, e.g. the location and timing of protein expression, and the host cell to be transformed, these being limitations inherent in the art of constructing recombinant DNA molecules. However, an expression vector useful in practicing the present invention is at least capable of directing the replication, and preferably also the expression of the polypeptide coding gene included in the DNA segment to which it is operatively linked.
In preferred embodiments, the expression vector used to express the genes includes a selection marker that is effective in a plant cell, preferably a drug resistance selection marker. A preferred drug resistance marker is the gene whose expression results in kanamycin resistance, i.e., the chimeric gene containing the nopaline synthase promoter, Tn5 neomycin phosphotransferase II and nopaline synthase 3′ nontranslated region described by Rogers et al., in Methods For Plant Molecular Biology, a Weissbach and H. Weissbach, eds., Academic Press Inc., San Diego, Calif. (1988). A useful plant expression vector is commercially available from Pharmacia, Piscataway, N.J.
Expression vectors and promoters for expressing foreign proteins in plants have been described in U.S. Pat. Nos. 5,188,642; 5,349,124; 5,352,605, and 5,034,322 which are hereby incorporated by reference.
A variety of methods have been developed to operatively link DNA to vectors via complementary cohesive termini. For instance, complementary homopolymer tracks can be added to the DNA segment to be inserted and to the vector DNA. The vector and DNA segment are then joined by hydrogen bonding between the complementary homopolymeric tails to form recombinant DNA molecules.
Alternatively, synthetic linkers containing one or more restriction endonuclease sites can be used to join the DNA segment to the expression vector. The synthetic linkers are attached to blunt-ended DNA segments by incubating the blunt-ended DNA segments with a large excess of synthetic linker molecules in the presence of an enzyme that is able to catalyze the ligation of blunt-ended DNA molecules, such as bacteria phage T4 DNA ligase. Thus, the products of the reaction are DNA segments carrying synthetic linker sequences at their ends. These DNA segments are then cleaved with the appropriate restriction endonuclease and ligated into an expression vector that has been cleaved with an enzyme that produces termini compatible with those of the synthetic linker. Synthetic linkers containing a variety of restriction endonuclease sites are commercially available from a number of sources including New England BioLabs, Beverly, Mass.
The nucleotide sequences encoding the protection protein and any other of the immunoglobulins of the present invention are introduced into the same plant cell either directly or by introducing each of the components into a plant cell and regenerating a plant and cross-hybridizing the various components to produce the final plant cell containing all the required components.
Any method may be used to introduce the nucleotide sequences encoding the components of the immunoglobulins of the present invention into a eukaryotic cell. For example, methods for introducing genes into plants include Agrobacterium-mediated plant transformation, protoplast transformation, gene transfer into pollen, injection into reproductive organs and injection into immature embryos. Each of these methods has distinct advantages and disadvantages. Thus, one particular method of introducing genes into a particular eukaryotic cell or plant species may not necessarily be the most effective for another eukaryotic cell or plant species.
Agrobacterium tumefaciens-mediated transfer is a widely applicable system for introducing genes into plant cells because the DNA can be introduced into whole plant tissues, bypassing the need for regeneration of an intact plant from a protoplast. The use of Agrobacterium-mediated expression vectors to introduce DNA into plant cells is well known in the art. See, for example, the methods described by Fraley et al., Biotechnology, 3:629 (1985) and Rogers et al., Methods in Enzymology, 153:253-277 (1987). Further, the integration of the Ti-DNA is a relatively precise process resulting in few rearrangements. The region of DNA to be transferred is defined by the border sequences and intervening DNA is usually inserted into the plant genome as described by Spielmann et al., Mol. Gen. Genet., 205:34 (1986) and Jorgensen et al., Mol. Gen. Genet., 207:471 (1987). Modern Agrobacterium transformation vectors are capable of replication in Escherichia coli as well as Agrobacterium, allowing for convenient manipulations as described by Klee et al., in Plant DNA Infectious Agents, T. Hohn and J. Schell, eds., Springer-Verlag, New York (1985) pp. 179-203. Further recent technological advances in vectors for Agrobacterium-mediated gene transfer have improved the arrangement of genes and restriction sites in the vectors to facilitate construction of vectors capable of expressing various polypeptide coding genes. The vectors described by Rogers et al., Methods in Enzymology, 153:253 (1987), have convenient multi-linker regions flanked by a promoter and a polyadenylation site for direct expression of inserted polypeptide coding genes and are suitable for present purposes.
Agrobacterium-mediated transformation of leaf disks and other tissues appears to be limited to plant species that Agrobacterium tumefaciens naturally infects. Thus, Agrobacterium-mediated transformation is most efficient in dicotyledonous plants. However, the transformation of Asparagus using Agrobacterium can also be achieved. See, for example, Bytebier, et al., Proc. Natl. Acad. Sci., 84:5345 (1987).
In those plant species where Agrobacterium-mediated transformation is efficient, it is the method of choice because of the facile and defined nature of the gene transfer. However, few monocots appear to be natural hosts for Agrobacterium, although transgenic plants have been produced in asparagus using Agrobacterium vectors as described by Bytebier et al., Proc. Natl. Acad. Sci. U.S.A., 84:5345 (1987). Therefore, commercially important cereal grains such as rice, corn, and wheat must be transformed using alternative methods. Transformation of plant protoplasts can be achieved using methods based on calcium phosphate precipitation, polyethylene glycol treatment, electroporation, and combinations of these treatments. See, for example, Potrykus et al., Mol. Gen. Genet., 199:183 (1985); Lorz et al., Mol. Gen. Genet., 199:178 (1985); Fromm et al., Nature, 319:791 (1986); Uchimiya et al., Mol. Gen. Genet., 204:204 (1986); Callis et al., Genes and Development, 1:1183 (1987); and Marcotte et al., Nature, 335:454 (1988).
Application of these systems to different plant species depends upon the ability to regenerate that particular plant species from protoplasts. Illustrative methods for the regeneration of cereals from protoplasts are described in Fujimura et al., Plant Tissue Culture Letters, 2:74 (1985); Toriyama et al., Theor Appl. Genet., 73:16 (1986); Yamada et al., Plant Cell Rep., 4:85 (1986); Abdullah et al., Biotechnology, 4:1087 (1986).
To transform plant species that cannot be successfully regenerated from protoplast, other ways to introduce DNA into intact cells or tissues can be utilized. For example, regeneration of cereals from immature embryos or explants can be effected as described by Vasil, Biotechnology, 6:397 (1988). In addition, “particle gun” or high-velocity microprojectile technology can be utilized as well. Using such technology, DNA is carried through the cell wall and into the cytoplasm on the surface of small (0.525 um) metal particles that have been accelerated to speeds of one to several hundred meters per second as described in Klein et al., Nature, 327:70 (1987); Klein et al., Proc. Natl. Acad. Sci. U.S.A., 85:8502 (1988); and McCabe et al., Biotechnology, 6:923 (1988). The metal particles penetrate through several layers of cells and thus allow the transformation of cells within tissue explants. Metal particles have been used to successfully transform corn cells and to produce fertile, stably transformed tobacco and soybean plants. Transformation of tissue explants eliminates the need for passage through a protoplast stage and thus speeds the production of transgenic plants.
DNA can be introduced into plants also by direct DNA transfer into pollen as described by Zhou et al., Methods in Enzymology, 101:433 (1983); D. Hess, Intern Rev. Cytol., 107:367 (1987); Luo et al., Plant Mol. Biol. Reporter, 6:165 (1988). Expression of polypeptide coding genes can be obtained by injection of the DNA into reproductive organs of a plant as described by Pena et al., Nature, 325:274 (1987). DNA can also be injected directly into the cells of immature embryos and the rehydration of desiccated embryos as described by Neuhaus et al., Theor. Apl. Genet., 75:30 (1987); and Benbrook et al., in Proceedings Bio Expo 1986, Butterworth, Stoneham, Mass., pp. 27-54 (1986).
The regeneration of plants from either single plant protoplasts or various explants is well known in the art. See, for example, Methods for Plant Molecular Biology, A. Weissbach and H. Weissbach, eds., Academic Press, Inc., San Diego, Calif. (1988). This regeneration and growth process includes the steps of selection of transformant cells and shoots, rooting the transformant shoots and growth of the plantlets in soil.
The regeneration of plants containing the foreign gene introduced by Agrobacterium tumefaciens from leaf explants can be achieved as described by Horsch et al., Science, 227:1229-1231 (1985). In this procedure, transformants are grown in the presence of a selection agent and in a medium that induces the regeneration of shoots in the plant species being transformed as described by Fraley et al., Proc. Natl. Acad. Sci. U.S.A., 80:4803 (1983). This procedure typically produces shoots within two to four weeks and these transformant shoots are then transferred to an appropriate root-inducing medium containing the selective agent and an antibiotic to prevent bacterial growth. Transformant shoots that rooted in the presence of the selective agent to form plantlets are then transplanted to soil to allow the production of roots. These procedures will vary depending upon the particular plant species employed, such variations being well known in the art.
The immunoglobulins of the present invention may be produced in any plant cell including plant cells derived from plants that are dicotyledonous or monocotyledonous, solanaceous, alfalfa, legumes, or tobacco.
Transgenic plants of the present invention can be produced from any sexually crossable plant species that can be transformed using any method known to those skilled in the art. Useful plant species are dicotyledons including tobacco, tomato, the legumes, alfalfa, oaks, and maples; monocotyledons including grasses, corn, grains, oats, wheat, and barley; and lower plants including gymnosperms, conifers, horsetails, club mosses, liver warts, horn warts, mosses, algaes, gametophytes, sporophytes of pteridophytes.
The plant cells of the present invention may in addition to the protection protein and the immunoglobulin derived heavy chain also contains a nucleotide sequence encoding an immunoglobulin derived light chain having at least a portion of an antigen binding domain.
The plant cells of the present invention may have an antigen binding domain that is capable of binding an antigen from S. mutans serotypes a, c, d, e, f, g, and h (S. mutans serotypes c, e, and f; and S. sobrinus serotypes d and g under new nomenclature) on the immunoglobulin derived heavy and light chains. The antigen binding domain present in these plant cells also can be able to bind to the responsible mucosal pathogens and prevent dental caries.
The plant cells of the present invention may be part of a plant and make up one of the following types of plants: dicotyledonous, monocotyledonous, solanaceous, alfalfa, tobacco or other type of plant.

D. Compositions Containing Immunoglobulins Having Protection Proteins

The present invention contemplates compositions of matter that comprise immunoglobulins of the present invention and plant macromolecules. Typically these plant macromolecules are derived from any plant useful in the present invention. The plant macromolecules are present together with an immunoglobulin of the present invention for example, in a plant cell, in an extract of a plant cell, or in a plant. Typical plant macromolecules associated with the immunoglobulins of the present invention in a composition are ribulose bisphosphate carboxylase, light harvesting complex, (LH6) pigments, secondary metabolites or chlorophyll. The compositions of the present invention have an immunoglobulin of the present invention present in a concentration of between 1% and 99% mass excluding water. Other preferred compositions include compositions having the immunoglobulins of the present invention present at a concentration of between 1% and 50% mass excluding water. Other preferred compositions include immunoglobulins at a concentration of 1% to 25% mass excluding water.
The compositions of the present invention contain plant macromolecules at a concentration of between 1% and 99% mass excluding water. Typically the mass present in the composition will consist of plant macromolecules and immunoglobulins of the present invention. When the immunoglobulins of the present invention are present at a higher or lower concentration the concentration of plant macromolecules present in the composition will vary inversely. In preferred embodiments the composition of plant macromolecules are present in a concentration of between 50% and 99% mass excluding water. In the most preferred compositions, the plant macromolecules are present in a concentration of between 75% and 99% mass excluding water.
The present invention contemplates a composition of matter comprising all or part of the following: an IgA heavy chain, a kappa or lambda chain, a J chain. These components form a complex and are attached to the protection protein as defined earlier. The composition also contains molecules derived from a plant. This composition may also be obtained after an extraction process yielding functional antibody and plant-derived molecules.
The extraction method comprises the steps of applying a force to a plant containing the complex whereby the apoplastic compartment of the plant is ruptured releasing said complex. The force involves shear, in dyn/cm2, as the primary method of releasing the apoplastic liquid.
The whole plant or plant extract contains an admixture of antibody and various other macromolecules of the plant. Among the macromolecules contained in the admixture is ribulose bisphosphate carboxylase (RuBisCo) or fragments of RuBisCo. Another macromolecule is LHCP. Another molecule is chlorophyll.
Shear force is a useful component of the overall force applied to the plant for disruption of apoplastic spaces. Other types of force may also be included to optimize the effects of shear. Direct pressure, for example, measured in lbs/in2, may enhance the effects of the apparatus used to apply shear. Commonly used homogenization techniques which are not appropriate for antibody extraction involve the use of high speed blades or cylinders which explosively destroy all plant structures.
The compositions of the present invention may contain an immunoglobulin of the present invention and plant molecules that are derived from a dicotyledonous, monocotyledonous, solanaceous, alfalfa, tobacco or other plant. The plant molecules present in the compositions of the present invention can be ribulose bisphosphate carboxylase, light harvesting complex, pigments, secondary metabolites, chlorophyll or other plant molecules.
Other useful methods for preparing composition containing immunoglobulins having protection protein include extraction with various solvents and application of vacuum to the plant material. The compositions of the present invention may contain immunoglobulins of the present in a concentration of between 1% and 99% mass excluding water. The compositions of the present invention may contain plant macromolecules in a concentration of between 1% and 99% mass excluding water.
Therapeutic compositions containing immunoglobulins of the present invention and plant macromolecules may be produced by processing a plant of the present invention by shearing under pressure a portion of that plant to produce a pulp containing the therapeutic immunoglobulin and plant macromolecules in a liquid derived from the apoplast or symplast of the plant which also contains the solid plant derived material. Further processing may be accomplished by separating the solid plant derived material from the plant derived liquid containing the immunoglobulins of the present invention. The starting material for such a process may include plant leaves, stem, roots, tubers, seeds, fruit or the entire plant. Typically, this processing is accomplished by a mechanical device which releases liquid from the apoplast or symplast of the plant. Additional processing steps may include separation of the solid plant derived material from the liquid using centrification settling flocculation or filtration. One skilled in the art will understand that these separation methods result in removing the solid plant derived material from the liquid including the immunoglobulins of the present invention. The methods of the present invention may produce immunoglobulins containing a protection protein and an immunoglobulin derived heavy chain that is comprised of domains or portions of immunoglobulin alpha chain and immunoglobulin gamma chain. The methods of the present invention may produce immunoglobulins containing a protection protein and an immunoglobulin derived light chain that is comprised of domains or portions of immunoglobulin kappa or lambda chain.
The methods of the present invention are operable on plant cells or part of a plant. The methods of the present invention may also included methods that further comprise growing the plant. The methods of the present invention may be applied to any plant including dicotyledonous, monocotyledonous, solanaceous, leguminous, alfalfa or tobacco plant. The methods of the present invention may be used to extract immunoglobulins from a portion of the plant such as a leaf, stem, root, tuber, seeds, fruit or entire plant. The methods of the present invention may use a mechanical device to shear the plants to release liquid from the apoplast or symplast of the plant. The plant pulp of the present invention may be separated to remove the solid plant material using one of the following methods: centrifugation, settling, flocculation or filtration.

E. Methods of Producing Immunoglobulins Containing Protection Proteins

The present invention contemplates methods of producing an immunoglobulin containing a protection protein comprising the steps of:

- (a) Introducing into the plant cell an expression vector containing a nucleotide sequence encoding a protection protein operatively linked to a transcriptional promoter; and
- (b) Introducing into the same plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain operatively linked to a transcriptional promoter.

The methods of the present invention optionally include introducing into the plant cell containing the expression vector with the nucleotide sequences for the protection protein and the immunoglobulin derived heavy chain a nucleotide sequence encoding an immunoglobulin derived light chain at least having a portion of an antigen binding domain operatively linked to a transcriptional promoter. Methods are also contemplated that introduce into a cell that already contains nucleotide sequences and promoters operatively linked to encode a protection protein and an immunoglobulin heavy chain and an immunoglobulin light chain, a promoter operatively linked to a nucleotide sequence encoding J chain. This results in a cell containing the nucleotide sequences operatively linked to promoters for an immunoglobulin heavy chain and an immunoglobulin light chain, J chain and a protection protein.
The plant cells of the present invention may be present as part of a plant that is capable of growth. Particularly useful plants for this invention include dicotyledonous, monocotyledonous, solanaceous, legumes, alfalfa, tomato, and tobacco plants.
The methods of the present invention include producing an assembled immunoglobulin having heavy, light and J chains and a protection protein within a eukaryotic cell. This eukaryotic cell is produced by introducing into that cell nucleotide sequences operatively linked for expression encoding an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain, an immunoglobulin derived light chain having at least a portion of an antigen binding domain, an immunoglobulin J chain, and a protection protein. These nucleotide sequences are operatively linked for expression by attaching appropriate promoters to each individual nucleotide sequence or to more than one nucleotide sequence thereby placing two nucleotide sequences encoding various molecules in tandem.
The eukaryotic cell produced by the present methods which contains these nucleotide sequences encoding the immunoglobulin heavy, light and J chains and the protection protein is maintained under conditions which allow those molecules to reproduce and assemble into an immunoglobulin which contains the protection proteins of the present invention.
The present invention also contemplates methods for making a particular immunoglobulin or antigen binding domain or domains of an immunoglobulin resistant to environmental conditions and more stable by operatively linking a nucleotide sequence encoding at least a portion of an antigen binding domain derived from an immunoglobulin heavy chain to a nucleotide sequence encoding at least one domain derived from an immunoglobulin α or μ heavy chain to form a nucleotide sequence encoding a chimeric immunoglobulin heavy chain. That nucleotide sequence encoding the chimeric immunoglobulin heavy chain is expressed in a eukaryotic cell which also contains at least one other molecule such as a protection protein, an immunoglobulin derived light chain having at least a portion of an antigen binding domain and an immunoglobulin J chain. In preferred embodiments, the cell contains all of the molecules including an immunoglobulin derived light chain having an antigen binding domain which is complementary to the antigen binding domain present on the immunoglobulin derived heavy chain. This method allows the chimeric immunoglobulin heavy chain to assemble with at least one other molecule, for example, the immunoglobulin derived light chain having the complementary antigen binding domain and an immunoglobulin J chain and the protection protein to form an immunoglobulin containing the protection protein which is resistant to environmental conditions.
These immunoglobulins are resistant to environmental conditions and thus more stable when subjected to elevated or reduced temperatures, high or low pH, high ionic or low ionic concentrations proteolytic enzymes and other harsh conditions. Such harsh conditions are typically found in the environment within natural water sources, within the human body, for example within the gut and on mucosal surfaces, and on the surface of an animal such as a mammal.

F. Chimeric Immunoglobulins Containing Protection Proteins

The present invention contemplates immunoglobulins containing a protection protein in which the immunoglobulin domains comprising the heavy and light chain are derived from different isotopes of either heavy or light chain immunoglobulins. One skilled in the art will understand that using molecular techniques these domains can be substituted for a similar domain and thus produce an immunoglobulin that is a hybrid between two different immunoglobulin molecules. These chimeric immunoglobulins allow immunoglobulins containing protection proteins to be constructed that contain a variety of different and desirable properties that are conferred by different immunoglobulin domains.
The present invention also contemplates chimeric immunoglobulins, including heavy, light and J chain which contain less than an entire domain derived from a different molecule. The same molecular techniques may be employed to produce such chimeric immunoglobulins.
In preferred embodiments, the immunoglobulins of the present invention contain at least the C_H1, C_H2, C_H3, domain of mouse IgG, IgG1, IgG2A, IgG2B, IgG3, IgA, IgE, or IgD. Other preferred embodiments of the present invention contain immunoglobulin domains that include at least the Cμ1, Cμ2, Cμ3, or Cμ4 domain of mouse IGM. Preferred immunoglobulins include immunoglobulins that contain the domains of Cε2, Cε3, and Cε4 of mouse immunoglobulin IGE.
The present invention also contemplates chimeric immunoglobulins derived from human immunoglobulins. These chimeric immunoglobulins contain domains from two different isotopes of human immunoglobulin. Preferred immunoglobulins include immunoglobulins that contain immunoglobulin domains including at least the C_H1, C_H2, or C_H3 of human IgG, IgG1, IgG2, IgG3, IgG4, IgA1, IgA2, IgE, or IgD. Other preferred immunoglobulins include immunoglobulins that contain domains from at least the C_H1, C_H2, C_H3, or C_H4 domain of human IgM or IgE The present invention also contemplates immunoglobulins that contain immunoglobulin domains derived from at least two different isotopes of mammalian immunoglobulins. Generally, any of the mammalian immunoglobulins can be used in the preferred embodiments, such as the following isotopes: any isotype of IgG, any isotype of IgA, IgE, IgD or IgM. The immunoglobulins of the present invention contained at least one of the constant region domains from two different isotopes of mammalian immunoglobulin.
The present invention also contemplates immunoglobulins that contain immunoglobulin domains derived from two different isotopes of rodent immunoglobulin. The isotopes of rodent immunoglobulin are well known in the art. The immunoglobulins of the present invention may contain immunoglobulin derived heavy chains that include at least one of the following immunoglobulin domains: the C_H1, C_H2, or C_H3 domain of a mouse IgG, IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, or IgD; the C_H1, C_H2, C_H3, C_H4 domain of mouse IgE or IgM; the C_H1, C_H2, or C_H3 domain of a human IgG, IgG1, IgG2, IgG3, IgG4, IgA1, IgA2, or IgD; the C_H1, C_H2, C_H3, C_H4 domain of human IgM or IgE; the C_H1, C_H2, or C_H3 domain of an isotype of mammalian IgG, an isotype of IgA, IgE, or IgD; the C_H1, C_H2, C_H3, C_H4 domain of a mammalian IgE or IgM; the C_H1, C_H2, or C_H3 domain of an isotype of rodent IgG, IgA, IgE, or IgD; the C_H1, C_H2, C_H3, C_H4 domain of a rodent IgE or IgM; the C_H1, C_H2, or C_H3 domain of an isotype of animal IgG, an isotype of IgA, IgE, or IgD; and the C_H1, C_H2, C_H3, C_H4 domain of an animal IgE or IgM. The present invention also contemplates the replacement or addition of protein domains derived from molecules that are members of the immunoglobulin superfamily. The molecules that belong to the immunoglobulin superfamily have amino acid residue sequence and nucleic acid sequence homology to immunoglobulins. The molecules that are part of the immunoglobulin superfamily can be identified by amino acid or nucleic acid sequence homology. See, for example, p. 361 of Immunoglobulin Genes, Academic Press (1989).

Tetratransgenic Organisms:

The present invention also contemplates a tetratransgenic organism which is comprised of cells having incorporated into the nucleic acid of that cell or plant within the cell four different transgenes, each encoding a different polypeptide. These transgenes are different in that the messenger RNA and polypeptides produced from that transgene are different from the messenger RNA and polypeptides produced from the other of the four transgenes. Thus, the number of transgenes referred to in the present invention does not include multiple copies of the same transgene as is commonly found in transgenic organisms. The present invention is directed to transgenic organisms having four transgenes which are not identical copies of other transgenes. The present invention does not exclude the possibility that each of the four different transgenes may be present in multiple copies. However, at least four separate transgenes that are different are present within the cells of the transgenic organism.
In addition, the present invention contemplates that four different transgenes are related in that the transgenes encode a polypeptide that is part of a multipolypeptide molecule. Therefore, the present invention contemplates that each individual polypeptide chain of a multipeptide molecule would be present on a transgene within a cell of the transgenic organism. The expression of each individual different polypeptide of the multipeptide molecule allows the different polypeptides to associate together to form the multipeptide molecule within the transgenic animal's cells. Thus, the present invention does not include within the four different transgenes in each individual cell, transgenes which encode polypeptides which do not associate together to perform a multipeptide molecule. Examples of such transgenes encoding molecules that do not associate together are polypeptides for antibiotic resistance such as kanamycin or neomycin or thymidine kinase.
In preferred embodiments, the transgenes present within a transgenic organism of the present invention encode the following four different polypeptides: a protection protein; an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain; an immunoglobulin derived light chain having at least a portion of an antigen binding domain; and an immunoglobulin J chain. In other preferred embodiments, one of the transgenes present in the transgenic organism encodes a chimeric immunoglobulin heavy, light or J chain. In other preferred embodiments, a transgene of the transgenic organisms of the present invention encode either an immunoglobulin heavy chain derived at least in part from an IgA or a IgM immunoglobulin. Other preferred embodiments include transgenic organisms containing transgenes which encode at least a portion of the amino acid sequence derived from an immunoglobulin heavy chain derived from either an IgA or IgM immunoglobulin heavy chain.
The present invention contemplates transgenic organisms including mammals, plants, rodents, reptiles, insects, amphibians, fishes or other organisms. In preferred embodiments, the transgenic organism of the present invention is a plant or a mammal. Methods of producing such organisms are well known. See, i.e., U.S. Pat. Nos. 4,736,866; 4,607,388; 4,870,009 and 4,873,191 which are hereby incorporated by reference.
The present invention also contemplates immunoglobulin that contain immunoglobulin derived heavy or immunoglobulin derived light chains that contain immunoglobulin domains which have been engineered to make those domains less immunogenic in a particular species. Typically, the immunoglobulin molecule is engineered as to be “humanized” in that it appears to be a human immunoglobulin even though derived from various other species.

EXAMPLES

The following examples illustrate the disclosed invention. These examples in no way limit the scope of the claimed invention.
1. Construction of DNA Vectors For Expression of Antibodies in Plants.
a. Isolation of the Nucleotide Sequences Encoding the Guy's 13 Immunoglobulin
Molecular cloning of the gamma and kappa chains of the Guy's 13 anti-S. mutans antibody was done by the procedures described in Ma et al., Eur. J. Immunol., 24:131 (1994). Briefly, mRNA was extracted from the Guy's 13 hybridoma cell line and converted to the cDNA by standard procedures. The cDNA was then amplified with the use of a pair of oligonucleotides specifically complementary to either the gamma or kappa cDNA. Amplification was catalyzed by Taq 1 polymerase using a thermal cycler as described. The amplified cDNAs were then digested with the appropriate restriction endonucleases and ligated into the corresponding restriction site in a standard plant expression vector. Numerous examples of such vectors have been reported in the literature and are generally available. An example of one vector that may be used is pBIN19.
In a related series of experiments, the cDNAs were cloned into the bacterial vector bluescript. Using this construct, the sequence of the gamma and kappa cDNAs was determined using the methods of Maxam and Gilbert.
Procedures for cloning antibody cDNAs involving PCR techniques or by construction of cDNA libraries followed by ligation of the obtained cDNAs into appropriate vectors are commonplace techniques which are familiar to one of ordinary skill in the art.
b) Hybrid cDNAs Encoding the Guy's 13 Heavy Chain Variable Region, a Part of the Gamma Chain Constant Region and a Part of an Alpha Chain Constant Region.
These constructs were synthesized as described in Ma et al., Eur. J. Immunol., 24:131 (1994) and ligated into the appropriate plant expression vectors as described above. The final construct had the structure: Guy's 13 variable region—(IgG1 C_H1)-(IgG1 C_H2)-(IgA C_H2)-(IgA C_H3), referred to as IgG2A heavy chain, and Guy's 13 variable region—(IgG1CH₁)-(IgACH2)-(IgACH3).
c) The Protection Protein and J Chain.
The cloned rabbit polyimmunoglobulin receptor (pIgR) cDNA was described by Mostov, Nature, 308:37 (1984) and shown in FIG. 8. The protection protein portion was obtained by PCR amplification of a portion of the nucleotide sequence coding for the (pIgR) and ligation into appropriate plant expression vectors as described above. The protection protein portion of the pIgR used in these constructs included the codon for amino acid number 1 to the codon for amino acid number 606. The method to accomplish this construction are well known in the art and the oligonucleotides can be selected using the pIgR nucleic acid sequence.
d) cDNAs Encoding Aglycosylated Derivatives of Heavy-Chain Constant Regions.
Mutagenesis procedures were performed either according to Stratagene protocols. In each case (i.e. alpha constant region, or protection protein) the codon for the asparagine utilized as the attachment site for carbohydrates, was changed to a codon for histidine.
2. Production of Transgenic Plants Expressing Therapeutic Antibodies.
Plants and plant cells containing immunoglobulins having a protection protein were produced in the following manner.
a) Transfer of Vectors to Agrobacterium tumefaciens
Plant transformation was accomplished by using Agrobacterium tumefaciens. E. coli DH5α bearing the recombinant pMON530 plant expression vector were mated with Agrobacterium in the presence of a helper strain (pRK2013) to provide transfer functions. Alternatively, pMON530 plasmid DNA was introduced into Agrobacteria by direct transformation. In this procedure, the Agrobacterium strain was first grown overnight at 28° C. in YEP medium. 2 ml of the overnight culture was used to inoculate 50 ml of YEP and was grown to an OD₆₀₀Of 1.0. The cells were then chilled to 4° C., pelletted by centrifugation and resuspended in 1 ml of ice cold 20 mM CaCl2. About 1 μg of DNA was added to aliquots of 0.1 ml of ice cold cells. The cells were then rapidly frozen by immersion in liquid nitrogen or in a dry ice ethanol bath. The cells were thawed by incubation at 37° C. for 5 minutes followed by the addition of 1 ml YEP medium. The cells were allowed to incubate for 2-4 hours with gentle shaking. Individual colonies carrying the recombinant vector were isolated by incubation on YEP agar plates containing the appropriate antibiotic.
Agrobacteria containing pMON530 were grown in media containing kanamycin, spectinomycin and chloramphenicol. Small segments of tobacco leaf were then co-cultivated with the Agrobacterium for 2 days after which the leaf segments were transferred to plates containing carbenicillin to kill the Agrobacterium. Regeneration of transformed leaf cells into whole plants was allowed to proceed in the presence of kanamycin selection until the plants were competent for growth in soil.
b) Regeneration of Transformed Tobacco and Petunia Plants.
Leaves from greenhouse grown tobacco or petunia plants were sterilized in 20% (by volume) Chlorox bleach, 0.1% sodium dodecyl sulfate at room temperature for 8 minutes. The leaves were then briefly rinsed in 70% ethanol and allowed to dry in sterile Petri plates.
Leaf discs of approximately 0.5 cm diameter were removed with a sterile hole puncher and placed on agar plates containing MS10 medium (MS10 medium per liter: 4.4 g Murashige and Skoog basal salts with minimal organics [Sigma #M68991, 30 g sucrose, 0.2 mg naphthalene acetic acid, 2 mg benzylaminopurine, 0.1 mg nicotinic acid, 0.1 mg pyridoxin, 0.1 mg thiamine, 10 g agar, pH 5.7 with KOH).
A 2 ml aliquot of a suspension of Agrobacterium in LB (approximately 1×10⁸ Agrobacteria per ml) was then added to the leaf pieces. All surfaces of the leaf discs were contacted with Agrobacteria, excess liquid was poured off the plate, and the discs were co-cultivated with the bacteria for 2 days at room temperature. The discs were then transferred to agar plates containing MS10 medium, 50 μg/ml kanamycin and 250 μg/ml carbenicillin (MS10-KC). Regeneration was allowed to proceed with weekly transfer of discs to fresh MS10-KC plates until regenerating shoots were visible. Shoots were then transferred to agar plates containing MSO-KC medium (MSO-KC per liter: 4.4 g Murashige and Skoog basal salts with minimal organics [Sigma #M68991, 30 g sucrose, 1 mg nicotinic acid, 1 mg pyridoxin, 0.1 mg thiamine, 50 μg/ml kanamycin and 250 μg/ml carbenicillin, 10 g agar, pH 5.7 with KOH).
After root formation, plantlets were transferred to soil and grown to maturity.
c) Regeneration of Transformed Alfalfa Plants.
Alfalfa trifoliates were cut from a greenhouse grown plant and sterilized in 20% (v/v) Chlorox bleach, 0.1% sodium dodecyl sulfate at room temperature for 8 minutes. The trifoliates were then briefly rinsed in 70% ethanol and allowed to dry in sterile Petri plates.
Leaf pieces of approximately 1 cm×4 mm were cut with a sterile scalpel and placed on agar plates containing B5H medium (B5H medium per liter: 3.1 g Gamborg's powdered medium (Sigma #G5893), 500 mg KNO3, 250 mg MgSO4 7H20, 30 g sucrose, 500 mg proline, 1 mg 2,4-dichlorophenoxyacetic acid, 100 μg kinetin, 100 mg inositol, 1 mg nicotinic add, 1 mg pyridoxin, 10 mg thiamine, 10 g agar, 30 ml stock amino acids, pH 5.7 with KOH; stock amino acids consist of 26.6 g L-glutamine, 3.32 g serine, 16.8 mg adenine, 333 mg glutathione per liter and are added after autoclaving when the medium is approximately 50° C.).
To the leaf pieces was then added 2 ml of a suspension of Agrobacterium in LB (approximately 1×10⁸ Agrobacteria per ml). All surfaces of the leaf were contacted with Agrobacteria, excess liquid was poured off the plate, and the leaves were co-cultivated with the bacteria for 2 days at room temperature. The leaf pieces were then transferred to agar plates containing B5H medium, 25 μg/ml kanamycin and 250 μg/ml carbenicillin (B5H-KC). Regeneration was allowed to proceed with weekly transfer of leaf pieces to fresh B5H-KC plates until somatic embryos were visible. Embryos were then transferred to agar plates containing BI02Y-KC medium (BI02Y-KC per liter: 25 ml macronutrients, 10 ml micronutrients, 25 ml iron, 1 ml vitamins, 1 ml aminos, 2 g yeast extract, 100 mg myo-inositol, 30 g sucrose, 10 g agar, 25 mg kanamycin, 250 mg carbenicillin, pH 5.9 with KOH; macronutrients consist of 40 g KNO3, 40 g NH4NO3, 13.88 g Ca(NO3)2-4FUO, 1.4 g MgSO4-7H20, 2.6 g KCl, 12 g Kh2PO4 per liter yielding a 40× stock; vitamins consist of 100 mg thiamine HCl, 500 mg nicotinic acid, 100 mg pyridoxin-HCl per liter yielding a 1000× stock; aminos consists of 2 g per liter glycine yielding a 1000× stock; micronutrients consist of 580 mg MnSO4-4H20, 1550 mg ZnSO4-7H20, 160 mg H3BO3, 80 mg KI per liter yielding a 100× stock; iron consists of 1.28 g NaFeEDTA per liter yielding a 40× stock).
After root formation, plantlets were transferred to soil and grown to maturity.
d) Regeneration of Transformed Tomato Plants.
Cotyledons from 7 day old tomato seedlings were sterilized in 20% (v/v) Chlorox bleach, 0.1% sodium dodecyl sulfate at room temperature for 8 minutes. The leaves were then briefly rinsed in 70% ethanol and allowed to dry in sterile Petri plates.
Cotyledon pieces of approximately 0.5 cm diameter were cut with a sterile scalpel and placed on agar plates containing MS4 medium (MS4 medium per liter: 4.4 g Murashige and Skoog basal salts with minimal organics [Sigma #M68991, 30 g sucrose, 2 mg zeatin riboside, 5 mg nicotinic acid, 0.5 mg pyridoxin, 0.5 mg thiamine, 1 mM acetosyringone, 10 g agar, pH 5.7 with KOH).
To the leaf pieces was then added 2 ml of a suspension of Agrobacterium in LB (approximately 1×10⁸ Agrobacteria per ml). All surfaces of the leaf discs were contacted with Agrobacteria, excess liquid was poured off the plate, and the discs were co-cultivated with the bacteria for 2 days at room temperature. The discs were then transferred to agar plates containing MS4 medium minus acetosyringone containing 50 μg/ml kanamycin and 250 μg/ml carbenicillin (MS4-KC). Regeneration was allowed to proceed with weekly transfer of discs to fresh MS4-KC plates until regenerating shoots were visible. Shoots were then transferred to agar plates containing MSO-KC medium (MSO-KC per liter: 4.4 g Murashige and Skoog basal salts with minimal organics [Sigma #M68991, 30 g sucrose, 1 mg nicotinic acid, 1 mg pyridoxin, 10 mg thiamine, 50 μg/ml kanamycin and 250 μg/ml carbenicillin, 10 g agar, pH 5.7 with KOH).
After root formation, plantlets were transferred to soil and grown to maturity.
e) Regeneration of Transformed Arabidopsis Plants.
Intact roots derived from Arabidopsis thalliana plants grown in sterile culture were first pretreated on callus inducing medium (CIM) for 3 days at 28° C. in the dark (CIM medium per liter: 3.1 g Gamborg's powdered medium (Sigma #G5893), 30 g sucrose, 1 mg 2,4-dichlorophenoxyacetic acid, 100 μg kinetin, 1 mg inositol, 0.1 mg nicotinic acid, 0.1 mg pyridoxin, 0.1 mg thiamine, 8 g agar, pH 5.7 with KOH).
To the intact roots was then added 2 ml of a suspension of Agrobacterium in LB (approximately 1×10⁸ Agrobacteria per ml). All surfaces of the roots were contacted with Agrobacteria and excess liquid was poured off the plate. The intact roots were then cut into 5 mm segments and were co-cultivated with the Agrobacteria for 2 days at 28° C. on CIM plates. The root pieces were then transferred to agar plates containing shoot inducing medium (SIM) containing 50 μg/ml kanamycin and 250 μg/ml carbenicillin (SIM medium per liter: 3.1 g Gamborg's powdered medium (Sigma #G5893), 30 g sucrose, 5 mg N⁶-(2-isopentenyl) adenine, 150 μg indole-3-acetic acid, 1 mg inositol, 0.1 mg nicotinic acid, 0.1 mg pyridoxin, 0.1 mg thiamine, 8 g agar, pH 5.7 with KOH).
Regeneration was allowed to proceed with weekly transfer of root pieces to fresh SIM plates until green regenerating shoots were visible. Shoots were then transferred to agar plates containing EM medium (MSO-KC per liter: 4.4 g Murashige and Skoog basal salts with minimal organics [Sigma #M6899], 10 g sucrose, 1 mg indole-3-butyric acid 1 mg nicotinic acid, 0.1 mg pyridoxin, 0.1 mg thiamine, 250 μg/ml carbenicillin, 8 g agar, pH 5.7 with KOH).
After root formation, plantlets were transferred to soil and grown to maturity.
3. Identification of Transgenic Plants.
Kanamycin resistant transformants expressing individual immunoglobulin chains were identified by ELISA as described. Further analysis of the transformants included evaluation of RNA by Northern blotting and evaluation of immunoglobulin polypeptides by Western blotting, both as described in Maniatis et al.
For each immunoglobulin chain, antigenic material, RNA or protein were detected by the respective assays. Transformants identified as having the highest levels of immunoglobulin chains were used in cross pollination protocols.
4. Assembly of Antibodies by Cross Pollination of Transformants.
Cross pollinations were performed in order to obtain plants co-expressing the various components of the desired antibodies. These crosses yielded alfalfa, tomato, tobacco and Arabidopsis plants containing the following assembled components, all of which also contained the Guy's 13 antigen binding domain.


Type of Antibody	Immunoglobulin Components

1	G1 heavy chain, kappa light chain
2	G2/A heavy chain, kappa light chain
3	G2/A heavy chain, kappa light chain,
	J chain
4	G1/A heavy chain, kappa light, J
	chain, protection protein
5	G1/A heavy chain Kappa light chain

5. Extraction and Evaluation of Guy's 13 Type 1, 2 and 3 & 4 Antibodies From Transgenic Plants.
a) Extraction and Enrichment of Antibody Contained in Leaf.
Leaf pieces were chopped into approximately 1 cm²pieces. The pieces were then added to a cold solution of TBS having 10 μg/ml leupeptin (1 ml TBS per gram of leaf) contained in a chilled porcelain mortar both at approximately 4° C. Plant liquid was extracted by pulverizing the pieces with a cold pestle using a circular motion and hand pressure. Pulverizing was continued until the pieces became a nearly uniform pulp (approximately 3 minutes of pulverizing). The pulp was centrifuged at 4° C. and approximately 50,000×g to yield a supernatant devoid of solid plant pieces. Alternatively, the pulp was filtered through a plastic mesh with a pore size of approximately 100 microns.
Depending on the titer of antibody contained in the particular plant, the supernatant was either directly suitable for exposure to antigen or required enrichment to a suitable concentration. Yields of IgG1's or IgG/A's in the crude extract were routinely less than 10 μg/ml and averaged approximately 5 μg/ml. For applications of a Guy's 13 antibody to mucosal surfaces, enrichment to a concentration of 1 to 4 mg/ml may be required. As a Type 1, 2 or 3 construct, Guy's 13 antibody required a ten to forty-fold enrichment to yield the desired concentration. This was accomplished either by affinity adsorption (utilizing either Protein A or Protein G), or by lyophilization to remove water. Size exclusion chromatography was also used for enrichment but required complete fractionation of the crude extract to yield an antibody of the required concentration. By ELISA assay and by polyacrylamide gel electrophoresis, the co-expressed chains assembled into a complex of approximately 180-200 k daltons for types 1 & 2 and approximately 400 k daltons for type 3. Crude extracts were routinely obtained containing approximately of 5-10 μg/ml.
A dramatic increase in antibody accumulation was observed when the protection protein was crossed into a plant containing Type 3 antibody yielding a plant containing a Type 4 antibody. By ELISA assay and by polyacrylamide gel electrophoresis, the co-expressed chains assembled into a complex of approximately 470,000 daltons. Crude extracts were routinely obtained containing in excess of 200 μg/ml with an average of approximately 250 μg/ml. Therefore, the SIgA construct of the Guy's 13 antibody required minimal enrichment to achieve the target concentration. This enrichment could be accomplished by the techniques described above. Alternatively, it was found that the antibody is readily separated from the majority of plant molecules by a one ultrafiltration step using membrane with a molecular exclusion of 200,000 d.
b) Functionality of the Guy's 13 Type 4 Antibody.
Functional antibody studies were carried out by ELISA. All plants expressing antibody light and heavy chains assembled functional antibody that specifically recognized streptococcal antigen (SA I/II). The levels of binding and titration curves were similar to those of mouse hybridoma cell supernatants. No SA I/II binding was detected with plants expressing only J chain or only protection protein. Likewise, wild-type plants expressing no immunoglobulin showed no detectable levels of binding.
In a similar set of experiments, binding of antibody to immobilized purified streptococcal antigen or native antigen on the bacterial cell surface was detected using an anti-secretory component antiserum. In these assays, only the Type 4 antibody binding was detected. The functional Type 1, 2 or 3 antibodies did not bind the anti-secretory component antiserum. These results confirm that the protection protein was assembled with antibody in the plants expressing Type 4 constructs and in a manner which did not interfere with antigen binding.
6. Expression of Chimeric Immunoglobulins.
The genes encoding the heavy and light chains of a murine monoclonal antibody (mAb Guy's 13) have been cloned and expressed in Nicotiana tabacum. Transgenic plants have been regenerated that secrete full-length Guy's 13 antibody. By manipulation of the heavy chain gene sequence, constant region domains from an immunoglobulin alpha heavy chain have been introduced, and plants secreting Guy's 13 mAb with chimeric gamma/alpha heavy chains have also been produced. For each plant antibody, light and heavy chains have been detected by Western blot analysis and the fidelity of assembly confirmed by demonstrating that the antibody is fully functional, by antigen binding studies. Furthermore, the plant antibodies retained the ability to aggregate streptococci, which confirms that the bivalent antigen-binding capacity of the full length antibodies is intact.
a) Cloning of Heavy and Light Chain Genes
Messenger RNA was purified from the Guy's 13 and a murine IgA (MOPC315) hybridoma cell line, using an acid guanidiniumthiocyanate-phenol-chloroform extraction. Complementary DNA was made using Moloney murine leukemia virus reverse transcriptase (Promega, GB). DNA encoding the gamma and kappa chains of Guy's 13 were amplified by polymerase chain reaction (PCR). The degenerate oligonucleotides used in the PCR were designed to incorporate a 5′ terminal XhoI, and a 3′-terminal EcoRI restriction site in the amplified DNA fragments. Following restriction enzyme digestion, the immunoglobulin chain encoding DNA was ligated into a constitutive plant expression vector (pMON 530), which contains a mouse immunoglobulin leader sequence upstream of the cloning site. The recombinant vector was used to transform E. coli (DH5-α, Gibco BRL) and screening was by Southern blotting, using radiolabeled DNA probes derived from the original PCR products. Plasmid DNA was purified from positive transformants and introduced into Agrobacterium tumefaciens.
A similar approach was used to construct two forms of a hybrid Guy's 13 heavy chain. The synthetic oligonucleotides shown in FIG. 1 were used in PCR to amplify the regions: (a) Guy's 13 signal sequence to the 3′ end of Cτ1 domain (J1-J5), (b) Guy's 13 signal sequence to the 3′ end of Cτ2 domain (J1-J2), and (c) 5′ end of Cα2 domain to the 3′ terminus of DNA from the MOPC 315 hybridoma (J3-J4). The fragments were purified (Geneclean II, Bio 101, La Jolla, Calif.) and digested with HindIII for 1 h at 37° C. The Guy's 13 fragments were ligated to the MOPC 315 fragment with T4 DNA ligase (Gibco, BRL), at 16° C. for 16 h, and an aliquot of the reaction mixture was used as template DNA for a further PCR, using the 5′ terminal oligonucleotide for Guy's 13 (J1) and the 3′ terminal oligonucleotide for MOPC 315 (J4). Amplified DNA fragments were purified and ligated into the pMON 530 vector as described above. The vector used in this procedure did not have a previously inserted mouse leader sequence, as in this case, the DNA encoding the native Guy's 13 leader sequence was included in the PCR amplification.
b) Plant Transformation and Regeneration
Leaf discs, about 6 mm in diameter, were cut from surface-sterilized tobacco leaves (Nicotiana tabacum, var. xanthii) and incubated overnight at 28° C., with a culture of the recombinant A. tumefaciens, containing immunoglobulin cDNA inserts. The discs were transferred to culture plates containing a medium that induces regeneration of shoots, supplemented with kanamycin (200 mg/l) and carbenicillin (500 mg/l). Shoots developing after this stage were excised and transplanted onto a root-inducing medium, supplemented with kanamycin (200 mg/l). Rooted plantlets were transplanted into soil as soon as possible after the appearance of roots. Plants were screened for expression of immunoglobulin chains as described below. Those that expressed heavy chains were crossed with those expressing light chains, by cross-pollination. The resulting seeds were sown in soil and allowed to germinate. Twenty-two transgenic plants were regenerated from transformations with light or heavy chain constructs, as determined by ELISA. Crossing of light and heavy chain-secreting plants resulted in 3/10 F1 progeny plants expressing kappa and gamma chains together, 4/17 plants expressing both kappa and the plant G1/A heavy chain and 3/8 plants expressing both kappa and the plant G2/A heavy chain together.
The three different forms of Guy's 13 monoclonal antibody expressed in plants, therefore, all contain the identical light (kappa) chain, but different heavy chains. These will be abbreviated throughout this report as follows (FIG. 1): Guy's 13 IgG1 with original gamma heavy chain, plant G13, Guy's 13 with IgG/IgA hybrid heavy chain consisting of var-τ1-τ2-α2-α3 domains, plant G2/A. The Guy's 13 hybridoma cell culture supernatant used as a positive control will be abbreviated to Mouse G13. Negative control plants were those that had been transformed with pMON 530 vector containing an insert that encodes an irrelevant mouse protein.
c) Antibody Chain Detection
Production of either gamma, kappa or the gamma/alpha chain hybrids was detected by ELISA. Microtiter wells were coated with a goat anti-mouse heavy or light chain-specific IgG (Fisher, USA; Sigma, GB; Nordic Pharmaceuticals, GB) in 150 mM NaCl, 20 mM Tris-HCl (pH 8) (TBS). Blocking was with 5% non-fat dry milk in TBS at 4° C. overnight. Plant leaves were homogenized in TBS with leupeptin (10 μg/ml) (Calbiochem, USA). The supernatant was added in serial twofold dilutions to the microtiter plate and incubation was at 4° C. overnight. After washing with TBS with 0.05% Tween 20, bound immunoglobulin chains were detected with the appropriate goat anti-mouse heavy or light chain-specific antibody, conjugated with horseradish peroxidase (Fisher; Sigma; Nordic Pharmaceuticals), for 2 h at 37° C. Detection was with 2,2′-azino-di-(3-ethyl-benzthiazoline-sulfonate) (Boehringer, FRG).
A similar assay was used to determine the concentrations of the murine and plant Guy's 13 antibodies. These were compared with a mouse IgG1 mAb (MOPC 21), and a mouse IgA mAb (TEPC 21) used at known concentrations (Sigma). ELISA plates were coated with an anti-mouse kappa antiserum. After blocking, bound antibody was detected with horseradish peroxidase-labeled anti-mouse gamma or alpha antiserum. Antibody concentration was determined by comparison of binding curves for each antibody.
ELISA was also used to detect the binding function of the assembled antibody. Binding to SA I/II was detected using microtiter plates that had been coated with purified SA I/II at an optimized concentration of 2 μg/ml. The ELISA procedure was as described above. The ability to bind S. mutans or E. coli cells was detected using intact cells (strains Guy's c, S. mutans and DH5-α, E. coli) that had been grown to stationary phase, for 18 h at 37° C. and fixed in 10% formalin. All the antibody solutions were adjusted to an initial concentration of 1.5 μg/ml and used in serial twofold dilutions. Extracts from plants expressing wither Guy's 13 heavy or light chain singly were also included in these assays, to determine if the single immunoglobulin chains exhibited any antigen-binding activity. Antibodies bound to either cells or purified SA I/II were detected using a horseradish peroxidase-conjugated goat anti-mouse light or heavy chain antiserum (Nordic Pharmaceuticals). The results are expressed as mean ±standard deviation of duplicate results from three separate assays.
Competition ELISA was performed on microtiter plates coated with purified SA I/II as above. The plates were incubated with plant extracts of Guy's 13 hybridoma supernatant at 1.5 μg/ml and serial twofold dilutions at 37° C. for 1 h and 4° C. overnight. After washing, ¹²⁵I-labeled mouse Guy's 13 was added and left to incubate for 2 h at 37° C. The plates were washed again and the bound radioactivity was counted in a gamma counter (Hydragamma 16, Innotec, GB). The results are expressed as % inhibition of labeled mouse Guy's 13 binding, in which 100% is the radioactive count from wells to which no blocking solution had been added.
d) Western Blot Analysis
Aliquots of 10 μl of leaf homogenates were boiled with 75 mM Tris-HCl (pH 6.8), 2% SDS, under reducing and nonreducing conditions. SDS-PAGE in 10% acrylamide was performed, and the gels were blotted onto nitrocellulose. The blots were incubated for 16 h in TBS with 0.05% Tween 20 and 1% non-fat dry milk, followed by goat anti-mouse IgG1, kappa (Nordic Pharmaceuticals) or alpha chain-specific antisera (Sigma), and incubated for 2 h at 37° C. After washing, the second-layer antibody, an alkaline phosphatase-conjugated rabbit anti-goat IgG (Sigma) was applied for 2 hours at 37° C. Antibody binding was detected by incubation with 300 μg/ml nitroblue tetrazolium and 15 p μg/ml 5-bromo-4-chloro-3-idolyl phosphate (Promega).
e) DNA Sequencing
The DNA sequence of each cloned immunoglobulin gene insert confirmed that no mutations had occurred during PCR amplification or the cloning procedures. The introduction of the HindIII site in the λ/γ hybrid heavy chains resulted in the predicted addition of the leucine residue between the Cγ2 and Cα2 domains in Plant G2/A and leucine-lysine between the Cγ1 and Cα2 domains in Plant G1/A. The additional Cγ2 domain in the Plant G2/A construct is predicted to increase the length of the heavy chain by 141 amino acid residues (approximately 12000 Da). The plant G1/A heavy chain in predicted to be slightly larger than the native Guy's 13 heavy chain, by 33 amino acids, approximately 3000 Da.
Plasmid DNA that was purified from positive transformants in E. coli was sequenced. The immunoglobulin gene inserts were excised and sub-cloned into Bluescript (Stratagene, USA). The DNA sequence was determined by a di-deoxy termination procedure (Sequenase, USB, USA).
f) Expression of Assembled Antibody
Western blot analysis on extracts from three representative F1 progeny plants was performed and reported in FIG. 2 of Ma et al., Eur. J. Immunol., 24:131-138 (1994). Samples run under reducing conditions demonstrate the presence of light (kappa) chain at approximately 25 Kd, in the mouse Guy's 13, as well as in the three transgenic plants, but not in the control plant. Guy's 13 heavy (gamma) chain was also detected in plant G13 at approximately 57 Kd, but not in the control plant extract. A single protein species was detected, unlike the hybridoma producing the Guy's 13 antibody cell culture supernatant, in which a two protein species was a consistent finding. The difference in the molecular size of the mouse heavy chains is probably due to glycosylation differences, and the result suggests that in plants the two heavy chains may be glycosylated in the same way.
The heavy chains of plant G1/A and G2/A were detected with an anti-alpha chain antiserum. Compared with the mouse Guy's 13 heavy chain, (approximately 57 Kd), the heavy chain of plant G1/A has a slightly higher relative molecular mass (approximately 60 Kd) and the plant G2/A heavy chain is much larger (approximately 70 Kd). This is consistent with the molecular weights predicted by sequence analysis. Several other protein species were detected in the transgenic plant extracts. These are likely to be proteolytic fragments of either light/heavy chain complexes, or of the heavy chain, as no bands were detected in the extract from the control transgenic plant. The anti-alpha chain antiserum did not cross-react with the mouse Guy's 13, which only contains gamma chain domains.
Samples were also run under nonreducing conditions to confirm the assembly of heavy and light chains into an immunoglobulin molecule and reported in FIG. 3 of Ma et al., Eur. J. Immunol., 24:131-138 (1994). Detection was with a labeled anti-kappa antiserum, and all three transgenic plants had assembled immunoglobulin at the correct M_rof above 150 Kd for full-length antibody. The plant G13 antibody has the same M_ras the mouse G13, but the plant G2/A and plant G1/A antibodies have higher M_ras predicted. A number of smaller proteolytic fragments were also detected, which is consistent with previous findings and the fact that a number of proteases are released by plants during the antibody extraction procedure. That these are antibody fragments, is confirmed by the absence of any detectable bands in the control plant extract.
g) Antigen Binding
Ten plants which were producing immunoglobulin were made in total, and the concentration of immunoglobulin in plant extracts varied between 1 and 10 μg/ml (mean 4.5 μg/ml). For the murine antibody and the representative plants used in this study, the concentrations estimated by ELISA were: mouse IgG-15.4 μg/ml, plant IgG-7.7 μg/ml, plant G1/A-1.5 μg/ml and plant G2/A-2.1 μg/ml. The concentrations determined for plant antibodies containing hybrid heavy chains are possibly underestimated, as they do not carry all of the constant region determinants, as compared with the standard mAb IgA used.
Titration curves for extracts from the three representative transgenic plants binding to SA I/II were generated and reported in FIG. 4 of Ma et al., Eur. J. Immunol., 24:131-138 (1994). Specific antibody was detectable in all three transgenic plant extracts, and the titration curves were similar to that of the murine hybridoma cell culture supernatant, used at the same concentration. The binding of the plant G1/A antibody appeared to be slightly lower than the other antibodies, although the titration curve followed a similar pattern. No SA I/II binding activity was detected in the negative control plant nor did extracts from plants individually expressing light or heavy chains have binding activity towards purified SA I/II. These findings demonstrate that the transgenic plants expressing both light and heavy chains have assembled the antibody molecule correctly to form a functional antigen binding site and that single light or heavy chains are not capable of binding the antigen.
The plant antibodies also recognized native antigen on the surface of streptococcal cells as shown in FIG. 5 of Ma et al., Eur. J. Immunol., 24:131-138 (1994) (S. mutans serotype c), which further confirms the integrity of the antigen-binding site in the plant antibodies. There were no significant differences between the binding of the different antibodies. Neither extracts from control plants, nor plants expressing only heavy or light chains showed any binding to S. mutans cells. There was no binding to E coli cells by any of the plant extracts, at concentrations of 1.0 and 0.5 μg/ml.
The plant antibodies competed with the original mouse Guy's 13 mbAb for binding to SA I/II. Up to 85% inhibition of ¹²⁵I-labeled mouse Guy's 13 mAb binding to SA I/II was demonstrated using the plant antibodies as shown in FIG. 6 of Ma et al., Eur. J. Immunol., 24:131-138 (1994). As before, the inhibition titration curves of the plant antibodies were similar to each other, and comparable to that of the mouse Guy's 13, whereas the control plant extract gave no inhibition.
h) Aggregation of S. mutans
The action of the immunoglobulin produced in plants having the Guy's 13 antigen binding region on bacteria was determined and reported in FIG. 7 of Ma et al., Eur. J. Immunol., 24:131-138 (1994). Plant extracts were sterilized by filtration through a 0.22 μm pore size filter and diluted tenfold with Todd Hewitt broth. The samples were inoculated with 0.05 vol of an overnight S. mutans culture and incubated at 37° C. overnight. The samples were Gram stained and examined under oil immersion microscopy. S. mutans grown in the presence of mouse Guy's 13, plant Guy's 13, plant G1/A or plant G2/A became aggregated and cell clumping was evident. However, the control plant extract had no effect on S. mutans growth. None of the plant mAb appeared to affect S. mutans rate of growth, as determined by culture of viable organisms at 8, 12 and 16 h. This result demonstrates not only that the plant antibodies have correctly assembled antigen-binding regions, but also that the antibody molecules bind antigen bivalently.

Example 7

Production of Immunoglobulins Containing Protection Proteins

Four transgenic Nicotiana tabacum plants were generated to express (1) a murine monoclonal immunoglobulin kappa chain having the antigen binding site of the Guy's 13 light chain, (2) a hybrid IgA/G murine immunoglobulin heavy chain containing Cγ and Cα chain domains and the antigen binding site of the Guy's 13 heavy chain, (3) a murine J chain and (4) protection protein comprised of amino acids 1-606 of rabbit polyimmunoglobulin receptor and did not contain amino acids 627-675 of the rabbit polyimmunoglobulin receptor. See, Example 1. Successive sexual crosses between these plants resulted in simultaneous expression of all four protein chains in the progeny plants. In some cases, back crossing was used to produce homozygous plants. The four recombinant polypeptides were assembled into a functional, high molecular weight immunoglobulin containing a protection protein of approximately 470,000 Kd. The assembly of the protection protein with the immunoglobulin was dependent on the presence of a J chain, as no association of the protection protein was detected when plants expressing antibody alone were crossed with those expressing the protection protein. Microscopic evaluation of plants expressing the immunoglobulins containing the protection protein demonstrated co-incident expression of protection protein and immunoglobulin heavy chains in single cells. Single cells are able to produce immunoglobulin having a protection protein in transgenic plants, whereas two cells are required for natural production of secretory immunoglobulin in mammals. The results demonstrate that sexual crossing of transgenic plants expressing recombinant sub-units is suitable for large scale production of immunoglobulin containing a protection protein for passive immunotherapy, as well as for expressing other complex protein molecules.
The immunoglobulin which contains the protection protein has the heavy and light chain antigen binding domains from the Guy's 13 monoclonal antibody that specifically recognize the cell surface adhesion molecule SA 1/11 of an oral streptococcus as shown by Smith, R. & Lehner, T. Oral Microbiol. Immunol. 4, 153-158 (1989). Transgenic immunoglobulin of this type containing only heavy and light chains has been generated in Nicotiana tabacum plants as described in Example 6. A mouse J chain construct containing the coding length cDNA was amplified using synthetic oligonucleotide primers corresponding to the N terminus MKTHLL and the C terminus SCYPD of mouse J chain as described by Matsuuchi, L., Cann, G. M. & Koshland, M. E. PNAS 83, 456-460 (1986). This amplified nucleotide sequence was ligated into a constitutive plant expression vector, pMON 530, that includes the 35S promoter from Cauliflower Mosaic Virus and has been described by Rogers, S. G., Klee, H. J., Horsch, R. B. & Fraley, R. T. Meth. Enzymol. 153, 253-276 (1987). Tobacco leaf tissue was transformed using agrobacterium containing the recombinant plasmid as described in the previous Examples. Regenerated plants were screened for the production of messenger RNA encoding J chain and positive transformants were self fertilized in order to generate homozygous progeny. The J chain expressing plants were crossed initially with those expressing the chimeric immunoglobulin heavy chain and kappa chain. Western blot analysis of the plant extract from plants expressing the chimeric immunoglobulin heavy chain with anti-kappa antiserum under non-reducing conditions, revealed a protein species of approximately 210 Kd, which is consistent with the presence of the extra constant region domains present in the chimeric immunoglobulin heavy chain, as compared with the original IgG1 antibody. The progeny from the cross between the plant expressing the immunoglobulin and a J chain plant resulted in the appearance of a major immunoglobulin band at approximately twice the relative molecular mass of approximately 400 Kd, demonstrating that assembly of the 3 polypeptides had occurred to form dimeric immunoglobulin (dlgA/G).
The protection protein construct consisted of a coding length cDNA amplified using synthetic oligonucleotide primers corresponding to the N terminus MALFLL and AVQSAE at amino acids 601-606 of the C terminus of rabbit polyimmunoglobulin receptor. The nucleotide sequence of the rabbit polyimmunoglobulin receptor was reported by Mostov, K. E., Friedlander, M. & Blobel, G. Nature 308, 37-43 (1984). The protection protein was generated in transgenic plants as described above and positive transformants expressing the protection protein were identified by Western blot analysis.
Plants expressing J chain assembled with the immunoglobulin having the IgA/G heavy chains to form dimers were then crossed with a homozygous plant expressing the protection protein. The progeny plants expressing the immunoglobulin having the protection protein contained a higher molecular weight protein species at approximately 470 Kd as determined by Western blot analysis under non-reducing conditions. This molecular size was consistent with that expected for an immunoglobulin containing a protection protein. This high molecular weight protein contained the protection protein as confirmed by Western blotting, using antiserum that specifically recognized the protection protein. The plant extracts also contained a protein species of approximately 400 Kd corresponding to the dimers of IgA/G and a protein species of approximately 210 Kd corresponding to the immunoglobulin with the chimeric heavy chain, but these were only detected by anti-kappa antiserum and not the anti-protection protein antiserum. In the transgenic plant producing the protection protein alone, there was no evidence that the protection protein assembled with endogenous plant proteins or formed multimers, as no high molecular weight proteins were detected in Western blotting under non-reducing conditions. Western blot analysis demonstrated that extracts from the plants expressing immunoglobulin heavy chain (IgA/G, dimeric IgA/G and the immunoglobulin containing a protection protein), but not the plants containing only the protection protein or J chain or wild-type plants, contained identical immunoglobulin derived heavy and light chains. Furthermore, only the plants containing protection proteins and the plants containing the IgG/A immunoglobulin having the protection protein expressed proteins that were recognized by the antiserum that specifically recognized the protection protein. No cross reacting proteins were detected in extracts from the wildtype control plant.
In mammals, the assembly of secretory component with the immunoglobulin requires the presence of J chain as described by Brandtzaeg, P. & Prydz, H. Nature 311, 71-73 (1984). Plants expressing immunoglobulins containing a chimeric heavy chain (IgA/G) were crossed with plants expressing protection protein. None of the 10 resulting progeny that expressed immunoglobulin and the protection protein without J chain produced assembled complexes as compared with the 10/10 plants that co-expressed J chain dimerized immunoglobulin and the protection protein without J chain, which assembled the M_r470 Kd immunoglobulin containing the protection protein. This confirms that J chain is required for the protection protein association with immunoglobulin as found in mammals. Only the approximately 210 Kd monomeric form of the immunoglobulin was recognized by anti-kappa antiserum, and the antisera that specifically bound the protection protein, recognized free protection protein, but no immunoglobulin heavy or light chains proteins.
Functional studies were carried out using the immunoglobulin produced in the 5 plant constructs using ELISA. All plants expressing immunoglobulin light and heavy chains, assembled functional immunoglobulin that specifically recognized streptococcal antigen (SA I/II). The levels of binding and titration curves were similar to those of the native mouse hybridoma cell supernatant. No SA I/II binding was detected in plants expressing only J chain or only protection protein or in wildtype plants. Binding of the immunoglobulins to immobilized purified streptococcal antigen or to native antigen on the bacterial cell surface was also detected using the antiserum which specifically binds the protection protein. In these assays, the binding of the immunoglobulin containing the protection protein to the streptococcal antigen was specifically detected. These results confirmed that the protection protein was assembled with the immunoglobulin to produce an immunoglobulin containing a protection protein in a manner which did not interfere with antigen binding.
The assembly of heavy and light chains into functional immunoglobulin molecules in plants is very efficient as shown by Hiatt, A. C., Cafferkey, R. & Bowdish, K. Nature 342, 76-78 (1989). A signal peptide must be present on both heavy and light chain constructs to direct the recombinant proteins to the endoplasmic reticulum antibody for assembly to take place in plants as was previously shown by Hiatt, A. C., Cafferkey, R. & Bowdish, K. Nature 342, 76-78 (1989). This study has demonstrated the fidelity of immunoglobulin assembly which includes dimerization of monomeric antibody by J chain in the transgenic plants. These results demonstrated that in plants the dimeric immunoglobulin population represents a major proportion (approx. 57%) of the total antibody. These results also demonstrate the production of an assembled immunoglobulin containing a protection protein which binds the corresponding antigen as well as the parent murine monoclonal antibody, which makes up a major proportion of the total antibody when the protection protein is incorporated (approximately 45%).
Co-expression of dimeric immunoglobulin with the protection protein in plants has led to assembly of a functional immunoglobulin containing a protection protein. All four transgenes for this complex protein were introduced into plants with the identical pMON530 expression cassette and native leader sequences. This vector contains a promoter sequence derived from the 35S transcript of the cauliflower mosaic virus which directs expression of transgenes in a variety of cell types of most plant organs as has been described by Benfey, P. N. & Chua, N-H. Science 250, 959-966 (1990); and Barnes, W. M. PNVAS 87, 9183-9187 (1990). Directing expression of all four transgenes with the same promoter maximized the likelihood of coincidental expression in a common plant cell. Microscopic observation of plants expressing an immunoglobulin containing a protection protein revealed that many cell types of the leaves contain the individual protein components that make up the immunoglobulin. These proteins accumulated at highest concentration in bundle sheath cells and were confined by the cell walls of these and other cells, but were not found in intercellular spaces. Restriction of the largest immunoglobulin components, the protection protein and the chimeric immunoglobulin heavy chain, within the confines of a protoplastic or apoplastic compartment of individual cells would constrain the assembly of the secretory immunoglobulin to those cells in which all the component molecules are synthesized. The subcellular site(s) and mechanism of assembly remain to be determined, assembly of IgG heterotetramers in plants requires targeting of both proteins to the endomembrane system as has been previously shown by Hiatt, A. C., Cafferkey, R. & Bowdish, K. Nature 342, 76-78 (1989); and Hein, M. B., Tang, Y., McLeod, D. A., Janda, K. D. & Matt, A. C. Biotechnol Prog. 7, 455-461 (1991).
In addition, we have demonstrated that a protection protein derived from mature secretory component devoid of signals for membrane integration, transcytosis or subsequent proteolysis can be assembled with chimeric immunoglobulin heavy chain containing immunoglobulin gamma and alpha protein domains. These results demonstrate that the inherent functions of IgG constant regions (protein A binding, complement fixation, Fc receptor activity) may be maintained in a dimeric immunoglobulin, capable of binding to a protective protein. These additional capabilities may be employed to enhance the function of an immunoglobulin used for passive immunotherapy and the development of plants capable of generating a functional immunoglobulin containing a protection protein will have significant implications in passive immunotherapy. The level of expression of the immunoglobulin containing a protection protein is high and the production can be scaled up to agricultural proportions, to allow economical production of monoclonal antibodies.

Methods

The following methods were used to prepare and analyze the Immunoglobulin of this Example.
i) Antibody Assembly in Transgenic Nicotiana tabacum.
Leaf segments were homogenized in 150 mM NaCl 20 mM Tris-HCl (pH8) (TBS), with leupeptin (10 μg/ml). The extracts were boiled for 3 minutes, in 75 mM Tris-HCl (pH6.8), 2% SDS, under non-reducing conditions and SDS-PAGE in 4% acrylamide was performed. The gels were blotted onto nitrocellulose. The blots were incubated for 2 hrs in TBS with 0.05% Tween 20 and 1% non-fat dry milk, followed by the appropriate antiserum and incubated for 2 hrs at 37° C. After washing, the second layer alkaline phosphatase conjugated antibody was applied for 2 hrs at 37° C. Antibody binding was detected by incubation with 300 mg/ml nitroblue tetrazolium and 150 mg/ml 5-bromo-4-chloro 3-indolyl phosphate.
These extracts were analyzed using western analysis to determine whether the immunoglobulins were assembled into immunoglobulin molecules by analyzing Western blots of plant extracts prepared under non-reducing conditions, were with anti-kappa antiserum (Bradsure, UK) and an antiserum which specifically recognizes protection protein. The immunoglobulins produced in the plants were compared to the monoclonal IgG1 Guys 13 immunoglobulin described by Smith, R. & Lehner, T. Oral Microbiol. Immunol. 4, 153-158 (1989).
ii) Western Analysis.
Western analysis was performed on each of the plant extracts prepared under reducing conditions to identify individual protein components of the immunoglobulin. Samples of the various plant extracts were prepared as described previously, but with the addition of 5% β-mercaptoethanol. SDS-PAGE in 10% acrylamide was performed and the protein in the gels transferred to nitrocellulose. Individual proteins were detected using anti-mouse γ1 heavy chain (Sigma, UK); anti-mouse kappa chain (Bradsure, UK); or an antiserum that specifically recognized the protection protein, followed by the appropriate alkaline phosphatase conjugated antibody.
iii) Western Analysis to Show Production of Immunoglobulin Having a Protection Protein
Western analysis of transgenic plant extract was performed as described in ii) above. The plant extracts from plants expressing the immunoglobulin containing the protection protein were subjected to SDS-PAGE under both non-reducing and reducing conditions and the proteins transferred to nitrocellulose. The immunoglobulin components were detected with an anti-kappa antiserum or with a sheep antiserum which specifically recognized the protection protein followed by an appropriate alkaline phosphatase labeled 2° antibody.
iv) Expression of Antigen-Specific Immunoglobulin Containing a Protection Protein in Transgenic Nicotiana tabacum.
To demonstrate that the plants were producing antigen-specific immunoglobulin, plant extract binding to purified streptococcal antigen (SA) I/II, detected with horseradish peroxidase labeled anti-kappa chain antiserum was determined. The presence of a protection protein in the antigen-specific immunoglobulin was demonstrated by plant extract binding to purified streptococcal antigen I/II and streptococcal cells detected with a sheep antiserum immunospecific for a protection protein, followed by alkaline phosphatase labeled donkey anti-sheep antiserum. These tests for antigen-specific immunoglobulin were carried out in microtitre plates that were coated with purified SA I/II (2 μg/ml) in TBS, or log phase growth Strep, mutans (NCTC 10449), in bicarbonate buffer (pH 9.8). Blocking was with 5% non-fat dry milk in TBS at room temperature for 2 hours. Plant leaves were homogenized in TBS with 10 μg/ml leupeptin (Calbiochem, USA). Mouse Guy's 13 hybridoma cell culture supernatant (IgG) was used as a positive control. The supernatants were added in serial two-fold dilutions to the microtitre plate and incubation was at room temperature for 2 hours. After washing with TBS with 0.05% Tween 20, bound immunoglobulin chains were detected with either a goat anti-mouse light chain specific antibody, conjugated with horseradish peroxidase (Nordic Pharmaceuticals, UK), or a sheep anti-SC antiserum, followed by an alkaline phosphatase labeled donkey anti-sheep antibody for 2 hours at room temperature. Detection was with 2,2′-azino-di-[3-ethyl-benzthiazolin-sulphonate (Boehringer, W. Germany) for HRPO conjugated antibody or disodium p-nitrophenyl phosphate (Sigma, UK) for alkaline phosphatase conjugated antibody.
v) Localization of Immunoglobulin Components in Plants
Photomicrographs of transgenic plants expressing immunoglobulins containing protection proteins and control Nicotiana tabacum leaf were prepared using immunogold detection of murine alpha chain. Briefly, leaf blades were cut into 2 mm×10 mm segments and fixed in 3% (w/v) paraformaldehyde, 0.5% (w/v) glutaraldehyde, 5% (w/v) sucrose in 100 mM sodium phosphate (pH 7.4). After dehydration in anhydrous ethanol, leaf segments were infiltrated with xylene, embedded in paraffin and cut into 3 mm sections and mounted on glass slides for immunochemical staining. The leaf sections were incubated with primary antibodies, affinity purified rabbit anti-mouse alpha chain (which reacts with the A/G hybrid heavy chain) or sheep anti-rabbit SC, and then with secondary antibody; goat anti-rabbit-10 mn gold or rabbit anti-sheep-10 mn gold. The immunogold signal was intensified by silver enhancement. The plants were visualized using both Phase contrast and bright field microscopy on the same leaf cross section. Immunolocalization of the protection protein on serial sections was used to show the same cellular localization for heavy chain as immunoglobulin. The analysis was carried out on the following cells and cell compartments: spongy mesophyll cells, epidermal cells, intercellular spaces, palisade parenchyma cells, and vascular bundles.
Further analysis of the exact localization of immunoglobulin components was carried out by analyzing serial sections of Nicotiana tabacum vascular bundle and control Nicotiana tabacum vascular bundle with immunogold detection for each of the components of the immunoglobulin. Serial sections of a transgenic plant leaves from plants expressing secretory immunoglobulin were incubated with an antibody that specifically recognizes the protection protein or with anti-IgA antibody followed by the appropriate gold-labeled secondary antibody. A control leaf section from a transgenic plant that did not contain any immunoglobulin coding sequences was also incubated with anti-IgA antibody, followed by gold-labeled goat anti-rabbit antiserum, or with the gold-labeled secondary antibodies alone and confirmed the specificity of staining. Both Phase contrast illumination of a minor vascular bundle and Bright field illumination of the same field were used to show immunogold localization of the protection protein. Bright field illumination of a serial leaf cross section of the vascular bundle demonstrated the same immunogold localization of the immunoglobulin heavy chain as was shown for the protection protein.

Example 8

Production of a Useful Plant Extract Containing Immunoglobulins Having a Protection Protein

Plant pieces (either leaf, stem, flower, root, or combinations) from plants producing immunoglobulins containing a protection protein were mixed with homogenization buffer (2 milliliter buffer per gram of plant material; homogenization buffer: 150 mM NaCl, 20 mM Tris-Cl, pH 7.5), homogenized into a pulp using a Waring blender and centrifuged at 10,000×g to remove debris. The supernatant was then extracted with an equal volume of HPLC-grade ethyl acetate by shaking at room temperature, followed by centrifugation at 10,000×g. The aqueous phase was transferred to another container, remaining ethyl acetate was removed from the aqueous phase by placing the solution under vacuum. The resulting crude extract consistently contained 100 μg immunoglobulin having a protection protein per ml. This method is useful for any plant containing an immunoglobulin having a protection protein.
A number of methods for homogenization have been used including a mortar and pestle or a Polytron and can be performed either in the cold or at room temperature.
The extract may be further purified by delipidation, by extraction with hexane or other organic solvents. Delipidation is not essential for deriving a useful product from the plant extract but is advantageous in cases where the final product is a purified immunoglobulin having a protection protein. In many instances the crude extract will contain a sufficiently high quantity of immunoglobulin having a protection protein (i.e. 100 μg/mL) to be useful without any further purification or enrichment. For an oral application, the extract would be mixed with commonly used flavorings and stabilizers. For a dental application, the extract would in addition be mixed with a gelling reagent to maintain contact of the extract with teeth. For a gastric application, the flavored extract could be swallowed directly.

Example 9

Stability of an Immunoglobulin Containing a Protection Protein

Two sets of crude plant extracts were prepared as described above. The first extract was derived from a plant expressing an IgG1 antibody and the second extract was derived from a plant expressing an immunoglobulin containing a protection protein. Crude plant extracts of this type from plants are known to contain a variety of proteolytic enzymes. Prolonged incubation of extracts at room temperature or at 37° C. therefore constitutes a proteolytic digestion.
Using ELISA the quantity of gamma-kappa complexes in the two extracts was determined as a function of time at both room temperature and 37° C. In these assays, an anti-kappa chain antibody was used to coat the plate followed by incubation with the plant extract at 37° C. for 1 hour. An anti-gamma chain antibody conjugated to HRPO was used for detection of immunoglobulin derived from the plant. The quantity of immunoglobulin having a protection protein contained in the extract immediately after the extract was prepared was taken to be 100%. After 3 hours at room temperature, the IgG1 contained 40% and the immunoglobulin containing the protection protein contained >95%. After 6 hours, the remaining IgG1 antibody was 20% and the immunoglobulin containing the protection protein abundance was still >95%. After 12 hours, there was no detectable IgG1 whereas ˜90% of the immunoglobulin containing the protection protein remained. A significant decrease (to ˜70%) in the abundance of protected antibody was not observed until 48 hours after the extract was prepared.

Example 10

Eukaryotic Tetratransgenic Cells Expressing Immunoglobulins Containing Protection Protein

The four chains comprising the immunoglobulin containing a protection protein can also be expressed in other cell types either in in vitro (cell cultures) or in vivo (transgenic animals). See, Manipulating the Mouse Embryo; A Laboratory Manual, B. Hogan et al., Cold Spring Harbor Laboratory (1986). In the case of transgenic animals, purified preparations of appropriate vector DNAs are adjusted to a final concentration of 2 ng/μl in 10 mM Tris, 0.2 mM EDTA, pH 7.4. Pronuclear injections are performed using zygotes prepared from inbred animals. Injected eggs are then transferred to pseudopregnant females using standard techniques. Live born animals are then screened for the presence of transgenes using any of a number of commonly used techniques such as PCR and ELISA. Members of the pedigree expressing different components of the immunoglobulin containing the protection protein are then mated to produce multi-transgene animals. Progeny from these crosses are then screened to identify those that express all four chains. Depending on the type of vector used for zygotic injections various cell types can be identified in the transgenic animals which assemble the complete immunoglobulin containing a protection protein. These vector DNAs can consist of specific promoter elements which allow transcription of the transgene in particular cell types or tissues. Each vector could express a single component of the protected antibody (IgG/A, J chain, protection protein, or kappa chain) or could potentially express more than one component. In this instance, the vector would contain an appropriate number of promoter regions and restriction sites to allow for transcription of each transgene.
Expression of all four chains in a cell culture system can be achieved using a DNA vector from which each component can be individually promoted. This would require four expression cassettes (containing promoter, multiple cloning site, and polyadenylation region) on the same vector DNA. Alternatively, individual cell lines can be sequentially transfected with individual vectors expressing single chains so long as each vector confers a selective resistance onto the cell line.
Commonly available vectors, such as pMAMneo (Clontech) can be adapted either for multiple expression or as a series of vectors expressing distinct selectable markers.
Transfection of any eukaryotic cells, such as fibroblasts, is done by conventional techniques. Briefly, cells are split 1:20 the day before transfection and are transfected at approximately 30% confluency using 125 mM CaCl2, 140 mM NaCl, 25 mM Hepes, 0.75 mM NaHPO4, pH 7.05, and 5 μg DNA/10 cm dish. After 16 hours of DNA incubation, cells are shocked by 10% dimethyl sulfoxide for 3 minutes. Forty eight hours after transfection, cells are subjected to selection by growth in the appropriate medium containing an antibiotic or other cytotoxic reagent.
The resulting cells produce all the components for the immunoglobulin containing the protection protein. These components are properly assembled to produce a functional immunoglobulin containing a protection protein.

Example 11

Engineering a Protection Protein Fused to a Portion of the Cytoplasmic Domain of the Rabbit Polyimmunoglobulin Receptor

The construction of DNA segments encoding a protection protein fused to a segment encoding a segment of the cytoplasmic domain of the rabbit polyimmunoglobulin receptor is produced as follows. Protection protein cDNA encoding from the first amino acid of the signal sequence (MET_—18) to GLU₆₀₆is ligated into any plant expression vector, such as the pMON530 vector (digested with Bgl II and Xho I) as a Bgl II-Xho I fragment. This protection protein derivative is obtained by PCR amplification using the appropriate oligonucleotide primers containing either a Bgl II or Xho I recognition sequence which are also complementary to DNA encoding residues −18 to −13 and residues 601 to 606 of the rabbit polyimmunoglobulin receptor respectively. The same procedure is performed in order to obtain a protection protein cDNA encoding from MET_—18to ALA₆₂₈except that the oligonucleotide containing an Xho site is also complementary to the protection protein cDNA encoding residues 623 to 628.
The cDNA encoding the rabbit polyimmunoglobulin receptor cytoplasmic domain fragment is obtained, also by PCR amplification, as a Xho I fragment. The oligonucleotides employed are complementary to DNA encoding from ARG₆₅₃to ALA₇₅₅both containing Xho I recognition sequences. This fragment is then ligated into the pMON530 vectors which contain the either of the protection protein cDNAs described above. The appropriate orientation of the cytoplasmic domain cDNA is determined by restriction digestions and by sequence analysis of plasmids obtained from transformed bacterial colonies.
The oligonucleotides employed for PCR amplification contain the appropriate number of nucleotides to ensure that the resulting cDNAs are in frame and capable of being translated as a continuous fusion protein containing both protection protein and cytoplasmic domain.
The resulting constructs in the appropriate orientation encode a protection protein fused directly to the polyimmunoglobulin receptor cytoplasmic domain with no functional transmembrane segment, operably linked to a DNA segment (promoter) enabling expression in a plant cell. The constructs encode two additional amino acids (SER-TRP) which are derived from introduction of the Xho I restriction site and which serve as a linker between the protection protein and the cytoplasmic domain.
These vectors are then used to transform Agrobacterium as previously described which in turn is used to transform plant cells. The same techniques described in the above Examples are used to produce a plant expressing this protein as part of an immunoglobulin.

Claims

1-94. (canceled)

95. An immunoglobulin comprising a protection protein in association with an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain.

96. The immunoglobulin of claim 95 further comprising an immunoglobulin derived light chain having at least a portion of an antigen binding domain associated with said immunoglobulin derived heavy chain.

97. The immunoglobulin of claim 95 further comprising a second immunoglobulin derived heavy chain having at least a portion of an antigen binding domain associated with said protection protein.

98. The immunoglobulin of claim 97 further comprising at least one immunoglobulin derived light chain having at least a portion of an antigen binding domain bound to said second immunoglobulin derived heavy chain.

99. The immunoglobulin of claim 95 further comprising an immunoglobulin J chain bound to said immunoglobulin derived heavy chain and optionally to a second immunoglobulin derived heavy chain.

100. The immunoglobulin of claim 95 that is a therapeutic immunoglobulin.

101. The immunoglobulin of claim 100 wherein said therapeutic immunoglobulin binds to mucosal pathogen antigens.

102. The immunoglobulin of claim 101 that is capable of preventing dental caries.

103. The immunoglobulin of claim 95 wherein said antigen binding domain is capable of binding an antigen from S. mutans serotypes c, e and f or S. sobrinus serotypes d and g.

104. The immunoglobulin of claim 95 wherein said protection protein has an amino acid sequence which substantially corresponds to at least a portion of the amino acid residues selected from the group consisting of 1 to 627 and 1 to 606 of the rabbit polyimmunoglobulin receptor and wherein said protection protein does not have an amino acid residue sequence corresponding to amino acid residues 628-755 of the rabbit polyimmunoglobulin receptor.

105. The immunoglobulin of claim 104 wherein said protection protein has an amino acid sequence which does not contain amino acid residues corresponding to amino acid residues 628 to 775 of the rabbit polyimmunoglobulin receptor and which does contain amino acid residues which correspond to one or more of the following amino acid segments:

a) amino acids corresponding to amino acid residues 21-43 of the rabbit polyimmunoglobulin receptor;

b) amino acids corresponding to amino acid residues 1-118 of the rabbit polyimmunoglobulin receptor;

c) amino acids corresponding to amino acid residues 119-223 of the rabbit polyimmunoglobulin receptor;

d) amino acids corresponding to amino acid residues 224-332 of the rabbit polyimmunoglobulin receptor;

e) amino acids corresponding to amino acid residues 333-441 of the rabbit polyimmunoglobulin receptor;

f) amino acids corresponding to amino acid residues 442-552 of the rabbit polyimmunoglobulin receptor;

g) amino acids corresponding to amino acid residues 553-606 or 553-627 of the rabbit polyimmunoglobulin receptor.

106. The immunoglobulin of claim 95 wherein said protection protein has an amino acid sequence which does not contain amino acid residues of a polyimmunoglobulin receptor of a species which are analogous to amino acid residues 628 to 755 of the rabbit polyimmunoglobulin receptor and which does contain amino acid residues from a polyimmunoglobulin receptor of a species which are analogous to one or more of the following amino acid segments:

107. The immunoglobulin of claim 106 wherein said species is human.

108. The immunoglobulin of claim 95 wherein said protection protein includes the amino acid sequence of at least one of the domains selected from the group consisting of the following portions of the rabbit polyimmunoglobulin receptor: domain I, domain II, domain III, domain IV, domain V, and amino acid residues 553 to 627 of domain VI; and does not have an amino acid sequence corresponding to amino acid residues 628-755 of the rabbit polyimmunoglobulin receptor.

109. The immunoglobulin of claim 95 wherein said protection protein does not have any amino acid sequence which corresponds to or is analogous to amino acid residues 628-755 of the rabbit polyimmunoglobulin receptor and which does include:

a) at least one domain which is from the polyimmunoglobulin receptor of a first animal and which is analogous to at least a portion of the following amino acid segments of the rabbit polyimmunoglobulin receptor: domain I, domain II, domain III, domain IV, domain V, and amino acid residues 553 to 627 of domain VI;

b) at least one domain which is from the polyimmunoglobulin receptor of a second animal and which corresponds to or is analogous to the following amino acid residue segments of the rabbit polyimmunoglobulin receptor: domain I, domain II, domain III, domain IV, domain V, and amino acid residues 553 to 627 of domain VI.

110. The immunoglobulin of claim 95 wherein said protection protein does not have any amino acid sequence which corresponds to or is analogous to amino acid residues 628-755 of the rabbit polyimmunoglobulin receptor and which does include:

a) at least one amino acid segment which is from the polyimmunoglobulin receptor of a first animal and which is analogous to at least a portion of the following amino acid residue segments of the rabbit polyimmunoglobulin receptor: domain I, domain II, domain III, domain IV, domain V, and amino acid residues 553 to 627 of domain VI;

b) at least one amino acid segment which is from the polyimmunoglobulin receptor of a second animal and which corresponds to or is analogous to the following amino acid residue segments of the rabbit polyimmunoglobulin receptor: domain I, domain II, domain III, domain IV, domain V, and amino acid residues 553 to 627 of domain VI.

111. The immunoglobulin of claim 110 wherein said first animal is a mammal and said second animal is a rabbit.

112. The immunoglobulin of claim 110 wherein said first animal is a human and said second animal is a rabbit.

113. The immunoglobulin of claim 95 wherein said immunoglobulin derived heavy chain contains at least a portion of an IgM or IgA heavy chain of any subtype.

114. The immunoglobulin of claim 95 wherein said immunoglobulin derived heavy chain is comprised of immunoglobulin domains from two different isotopes of immunoglobulin.

115. The immunoglobulin of claim 114 wherein said immunoglobulin domains are selected from the group consisting of:

a) the C_H1 of a mouse IgG1 and the C_H2 and C_H3 of mouse IgA; and

b) the C_H1 and C_H2 of a mouse IgG1 and the C_H2 and C_H3 of mouse IgA;

116. The immunoglobulin of claim 95 wherein said antigen binding domain substantially corresponds to the Guy's 13 heavy chain variable region.

117. The immunoglobulin of claim 96 wherein said antigen binding domain substantially corresponds to the Guy's 13 light chain variable region.

118. A composition comprising the immunoglobulin of claim 95 and at least one plant macromolecule.

119. The composition of claim 118 wherein said plant macromolecule is derived from a dicotyledonous, monocotyledonous, solanaceous, alfalfa or tobacco plant.

120. The composition of claim 118 wherein said plant macromolecule is selected from the group consisting of ribulose bisphosphate carboxylase, light harvesting complex, pigments, secondary metabolites and chlorophyll.

121. The composition of claim 118 wherein said immunoglobulin is present in a concentration of between 0.001% and 99% mass excluding water.

122. The composition of claim 119 wherein said plant macromolecules are present in a concentration of between 1% and 99% mass excluding water.

123. A method of producing the immunoglobulin of claim 95 comprising the steps of:

(a) introducing into a plant cell an expression vector containing a nucleotide sequence encoding a protection protein operably linked to a transcriptional promoter; and

(b) introducing into said plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain operably linked to a transcriptional promoter.

124. The method of claim 123 further comprising the step of:

(c) introducing into said plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin derived light chain having at least a portion of an antigen binding domain operably linked to a transcriptional promoter.

125. The method of claim 123 further comprising the step of introducing into said plant cell an expression vector containing a nucleotide sequence encoding an immunoglobulin J chain operably linked to a transcriptional promoter.

126. The method of claim 123 wherein said immunoglobulin derived heavy chain is immunoglobulin alpha chain and said immunoglobulin derived light chain is an immunoglobulin kappa or lambda chain.

127. The method of claim 123 wherein said immunoglobulin derived heavy chain is comprised of portions of immunoglobulin alpha chain and immunoglobulin gamma chain.

128. The method of claim 123 wherein said plant cells are part of a plant.

129. The method of claim 123 further comprising growing said plant cells into a regenerated plant.

130. The method of claim 128 wherein said plant is a dicotyledonous, monocotyledonous, solanaceous, leguminous, alfalfa or tobacco plant.

131. The method of claim 123 wherein said immunoglobulin derived heavy chain is a chimeric immunoglobulin heavy chain.

132. A method of producing a therapeutic immunoglobulin composition containing plant macromolecules, said method comprising the step of shearing under pressure plants or parts thereof to produce a pulp containing a therapeutic immunoglobulin and plant macromolecule mixture, said immunoglobulin comprising a protection protein, and wherein said immunoglobulin is encoded by at least one nucleic acid sequence that has been introduced into the cells of said plants.

133. The method of claim 132 further comprising the step of separating said solid plant derived material from said liquid.

134. The method of claim 132 wherein said portion of said plant is a leaf, stem, root, tuber, fruit or entire plant.

135. The method of claim 132 wherein said shearing is accomplished by a mechanical device which releases liquid from the apoplast or symplast of said plant.

136. The method of claim 133 wherein said separation is by centrifugation, settling, flocculation or filtration.

137. A method for producing an assembled immunoglobulin molecule having heavy, light and J chains and a protection protein comprising the steps of:

a) introducing into a eukaryotic cell nucleotide sequences operably linked for expression encoding:

i) an immunoglobulin derived heavy chain having at least a portion of an antigen binding domain,

ii) an immunoglobulin derived light chain having at least a portion of an antigen binding domain,

iii) an immunoglobulin J chain, and

iv) a protection protein; and

b) maintaining said cell under conditions allowing production and assembly of said immunoglobulin derived heavy and light chains, said immunoglobulin J chain and said protection protein into an immunoglobulin molecule.

138. A method for producing an assembled immunoglobulin molecule having heavy, light and J chains and a protection protein by maintaining under conditions allowing protein production and immunoglobulin assembly, a eukaryotic cell containing nucleotide sequences operably linked for expression encoding:

iii) an immunoglobulin J chain, and

iv) a protection protein.

139. The method of claim 137 wherein said eukaryotic cell is a plant cell.

140. A method of making an immunoglobulin resistant to environmental conditions comprising the steps of:

a) operably linking a nucleotide sequence encoding at least a portion of the antigen binding domain derived from an immunoglobulin heavy chain to a nucleotide sequence encoding at least one domain derived from an immunoglobulin alpha heavy chain to form a nucleotide sequence encoding a chimeric immunoglobulin heavy chain;

b) expressing said nucleotide sequence encoding said chimeric immunoglobulin heavy chain to produce said chimeric immunoglobulin heavy chain in a plant cell which also contains at least one other molecule selected from the group consisting of: a protection protein, an immunoglobulin derived light chain having at least a portion of an antigen binding domain and an immunoglobulin J chain; and

thereby allowing the chimeric immunoglobulin heavy chain to assemble with said at least one other molecule to form said immunoglobulin resistant to said environmental conditions.

141. The method of claim 140 wherein said other molecule is a protection protein and said plant cell also contains an immunoglobulin derived light chain having at least a portion of an antigen binding domain and an immunoglobulin J chain.

142. A process for producing an immunoglobulin resistant to environmental conditions by maintaining under conditions allowing protein production and immunoglobulin assembly a plant cell containing:

a) a nucleotide sequence encoding a chimeric immunoglobulin heavy chain in which a nucleotide sequence encoding at least a portion of an antigen binding domain derived from heavy chain is operably linked to a nucleotide sequence encoding at least one domain derived from an immunoglobulin alpha heavy chain; and

b) at least one other molecule selected from the group consisting of: a protection protein, an immunoglobulin derived light chain having at least a portion of an antigen binding domain and an immunoglobulin J chain; thereby allowing the chimeric immunoglobulin heavy chain to assemble with said at least one other molecule to form said immunoglobulin resistant to said environmental conditions.

143. The immunoglobulin of claim 95 wherein said chimeric immunoglobulin heavy chain contains an immunoglobulin domain from one of the following immunoglobulin heavy chains: IgG, IgA, IgM, IgE, IgD; and also contains a protection protein-binding domain from IgA or IgM.

144. The immunoglobulin of claim 143 wherein said immunoglobulin heavy chains are human, rodent, rabbit, bovine, ovine, caprine, fowl, canine, feline or primate immunoglobulin heavy chains.

145. The immunoglobulin of claim 143 wherein said protection protein-binding domain is from the IgA of a human, rodent, rabbit, bovine, ovine, canine, feline or primate.

146. The immunoglobulin of claim 143 wherein said chimeric immunoglobulin heavy chain is comprised of immunoglobulin chains of mouse IgG1 and said protection protein-binding domain is from mouse IgA or IgM.

147. The immunoglobulin of claim 143 wherein said chimeric immunoglobulin heavy chain is comprised of immunoglobulin domains of a human IgG, IgM, IgD or IgE and said protection protein-binding domain is from a human IgA or IgM.