US20060269961A1 - Dendrimer-based DNA extraction methods and biochips - Google Patents

Dendrimer-based DNA extraction methods and biochips Download PDF

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US20060269961A1
US20060269961A1 US11/501,900 US50190006A US2006269961A1 US 20060269961 A1 US20060269961 A1 US 20060269961A1 US 50190006 A US50190006 A US 50190006A US 2006269961 A1 US2006269961 A1 US 2006269961A1
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Prior art keywords
dendrimer
molecules
flow channel
bound
biochip
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US11/501,900
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Kazuhisa Fukushima
Saya Satou
Tadashi Matsunaga
Haruko Takeyama
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Yokogawa Electric Corp
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Yokogawa Electric Corp
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Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Definitions

  • the present invention relates to a method and a biochip for efficiently retrieving (or alternatively referred to as “extracting”) biopolymers such as DNA, RNA and protein from affected cells, for example, by producing dendrimer molecules in the flow channel of the biochip's preprocessing area.
  • the prior art is explained hereinafter by taking DNA as an example of a biopolymer.
  • the target DNA used for DNA chips is retrieved by means of preprocessing.
  • Methods of retrieving DNA from affected cells or the like dissolved in blood serum, lymphocyte-clastic solution or lysis buffer solution, for example, are roughly classified into two types: the centrifugal separation method and the magnetic bead method.
  • the centrifugal separation method involves the use of large-scale apparatus, the magnetic bead method is expected to become mainstream in the future where downsizing is required.
  • magnetic bead method is discussed hereinafter.
  • non-patent document 1 for example, for details on examples of applications of magnetic beads.
  • the material on which this method is based is referred to not only as magnetic bead, but also as magnetic particle or magnetic body.
  • patent document 1 for example, for details on examples of applications of the method in which the material is referred to as magnetic particles.
  • patent document 2 for example, for details on examples of applications of the method in which the material is referred to as magnetic body.
  • the magnetic bead method is a DNA retrieval method in which molecules of probe DNA or probe antibody are bound to the surfaces of magnetic beads at a specific density, and DNA in a solution is retrieved by means of a complementary combination of target DNA molecules in the solution and probe molecules. Then the magnetic beads are collected and cleaned using a magnet and the DNA is dissociated from the surfaces of the magnetic beads using a solution and is thus retrieved.
  • Patent Document 1
  • Patent Document 2
  • the magnetic bead method has difficulty in binding probes to the surfaces of magnetic beads at an appropriate density.
  • the efficiency of DNA retrieval is currently at issue.
  • the present invention is intended to solve the aforementioned problems.
  • One object of the present invention therefore, is to provide a method for retrieving biopolymers in a highly efficient manner by taking advantage of the dendrimers' ability to achieve highly efficient densities because of their freely controllable structural density, as well as a biochip using dendrimers.
  • Another object of the present invention is to provide a dendrimer-based biochip that has no mechanical moving parts and whose preprocessing area can be easily miniaturized.
  • FIG. 1 is a schematic view illustrating one embodiment of a flow channel formed in the preprocessing area of a dendrimer-based biochip in accordance with the present invention.
  • FIG. 2 is a schematic view illustrating another embodiment of the flow channel.
  • a dendrimer structure is formed in a flow channel through which a DNA solution flows, by taking advantage of the ability of dendrimers to achieve highly efficient densities because of their freely controllable structural density. Then, probe polymer molecules complementary with target biopolymer molecules are bound to the tips of dendrimers so that these biopolymer molecules are retrieved in a highly efficient manner.
  • the conventional method using magnetic beads requires mechanical moving parts since magnetic beads are first collected and then DNA is retrieved from their surfaces. The present invention eliminates the need for such mechanical moving parts, thereby enabling the preprocessing area to be easily miniaturized.
  • FIG. 1 is a schematic view illustrating one embodiment of a flow channel formed in the preprocessing area of a dendrimer-based biochip in accordance with the present invention, wherein FIG. 1 ( a ) is a perspective projection (partially perspective view) of the surfaces of a substrate, and FIG. 1 ( b ) is an enlarged view of a dendrimer molecule.
  • numeral 1 denotes the substrate of a biochip and numeral 2 denotes a flow channel through which a DNA solution flows, and numeral 3 denotes a dendrimer molecule bound to flow channel 2 .
  • Substrate 1 is made of such materials as glass or plastics and U-shaped flow channel 2 is formed in substrate 1 .
  • a plurality of molecules of dendrimer 3 is formed on the walls of flow channel 2 .
  • Dendrimer 3 is, for example, a multibranched polyamide amine dendrimer and is produced by providing aminosilane treatment on the surfaces of flow channel 2 and overlaying a film of amidoamine, which is produced by the reaction of methyl acrylate with ethylenediamine, upon the aminosilane-treated area as a dendron unit.
  • Probe DNA is bound to the tip (surface) of dendrimer 3 which goes into a complementary combination with target DNA.
  • the density of probe DNA molecules bound to the tips of dendrimer molecules has the optimum value, depending on the type of target (DNA, mRNA or protein). This optimum value can be obtained by changing the number of dendrimer layers (generations). Under normal conditions, second or higher generation dendrimers are used.
  • a plurality of concavities and convexities 4 may be formed on the walls of flow channel 2 so that turbulence takes place when a solution containing target DNA flows through flow channel 2 , as illustrated in FIG. 2 . This turbulence further increases the efficiency of the complementary combination between target DNA and probe DNA.
  • DNA segments are bound to the surfaces of dendrimer molecules and target and probe molecules are combined by means of hybridization.
  • molecules of a biopolymer probe other than DNA may be bound so that target molecules combine with the probe molecules.
  • antibody molecules may be bound to the surfaces of dendrimer molecules as probe molecules so that the protein of target molecules is extracted by means of antigen-antibody reaction.

Abstract

The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.

Description

  • This application is a divisional of application Ser. No. 10/928,183, filed Aug. 30, 2004.
    • BACKGROUND OF THE INVENTION
  • 1. Field of the Invention
  • The present invention relates to a method and a biochip for efficiently retrieving (or alternatively referred to as “extracting”) biopolymers such as DNA, RNA and protein from affected cells, for example, by producing dendrimer molecules in the flow channel of the biochip's preprocessing area.
  • 2. Description of the Prior Art
  • The prior art is explained hereinafter by taking DNA as an example of a biopolymer. The target DNA used for DNA chips is retrieved by means of preprocessing.
  • Methods of retrieving DNA from affected cells or the like dissolved in blood serum, lymphocyte-clastic solution or lysis buffer solution, for example, are roughly classified into two types: the centrifugal separation method and the magnetic bead method.
  • Since the centrifugal separation method involves the use of large-scale apparatus, the magnetic bead method is expected to become mainstream in the future where downsizing is required.
  • Accordingly, the magnetic bead method is discussed hereinafter. (See non-patent document 1, for example, for details on examples of applications of magnetic beads. It should be noted that the material on which this method is based, is referred to not only as magnetic bead, but also as magnetic particle or magnetic body. See patent document 1, for example, for details on examples of applications of the method in which the material is referred to as magnetic particles. Likewise, see patent document 2, for example, for details on examples of applications of the method in which the material is referred to as magnetic body.)
  • The magnetic bead method is a DNA retrieval method in which molecules of probe DNA or probe antibody are bound to the surfaces of magnetic beads at a specific density, and DNA in a solution is retrieved by means of a complementary combination of target DNA molecules in the solution and probe molecules. Then the magnetic beads are collected and cleaned using a magnet and the DNA is dissociated from the surfaces of the magnetic beads using a solution and is thus retrieved.
  • Non-Patent Document 1
  • Haruko Takeyama and Hideki Nakayaka, Chapter 7 “DNA Chips Using Magnetic Beads” in “DNA Chips and It's Application” published by CMC Publishing Co., Ltd. in July 2000 under the editorship of Tadashi Matsunaga
  • Patent Document 1
  • Japanese Laid-open Patent Application 1996-176212
  • Patent Document 2
  • Japanese Laid-open Patent Application 1999-313670
  • However, while apparatus using the magnetic bead method is smaller and more convenient than those using the centrifugal separation method, the magnetic bead method has difficulty in binding probes to the surfaces of magnetic beads at an appropriate density. Thus, the efficiency of DNA retrieval is currently at issue. In addition, it is cumbersome to collect magnetic beads with a magnet and then retrieve DNA from the surfaces of the beads. Therefore, an even simpler method of DNA retrieval is required in order to transform the preprocessing area into a chip in the future.
    • SUMMARY OF THE INVENTION
  • The present invention is intended to solve the aforementioned problems. One object of the present invention therefore, is to provide a method for retrieving biopolymers in a highly efficient manner by taking advantage of the dendrimers' ability to achieve highly efficient densities because of their freely controllable structural density, as well as a biochip using dendrimers.
  • Another object of the present invention is to provide a dendrimer-based biochip that has no mechanical moving parts and whose preprocessing area can be easily miniaturized.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1 is a schematic view illustrating one embodiment of a flow channel formed in the preprocessing area of a dendrimer-based biochip in accordance with the present invention.
  • FIG. 2 is a schematic view illustrating another embodiment of the flow channel.
    • DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • In the present invention, a dendrimer structure is formed in a flow channel through which a DNA solution flows, by taking advantage of the ability of dendrimers to achieve highly efficient densities because of their freely controllable structural density. Then, probe polymer molecules complementary with target biopolymer molecules are bound to the tips of dendrimers so that these biopolymer molecules are retrieved in a highly efficient manner. In addition, the conventional method using magnetic beads requires mechanical moving parts since magnetic beads are first collected and then DNA is retrieved from their surfaces. The present invention eliminates the need for such mechanical moving parts, thereby enabling the preprocessing area to be easily miniaturized.
  • The present invention will hereinafter be described in detail with reference to the accompanying drawings. FIG. 1 is a schematic view illustrating one embodiment of a flow channel formed in the preprocessing area of a dendrimer-based biochip in accordance with the present invention, wherein FIG. 1(a) is a perspective projection (partially perspective view) of the surfaces of a substrate, and FIG. 1(b) is an enlarged view of a dendrimer molecule.
  • In FIG. 1, numeral 1 denotes the substrate of a biochip and numeral 2 denotes a flow channel through which a DNA solution flows, and numeral 3 denotes a dendrimer molecule bound to flow channel 2.
  • Substrate 1 is made of such materials as glass or plastics and U-shaped flow channel 2 is formed in substrate 1. A plurality of molecules of dendrimer 3 is formed on the walls of flow channel 2.
  • Dendrimer 3 is, for example, a multibranched polyamide amine dendrimer and is produced by providing aminosilane treatment on the surfaces of flow channel 2 and overlaying a film of amidoamine, which is produced by the reaction of methyl acrylate with ethylenediamine, upon the aminosilane-treated area as a dendron unit.
  • Probe DNA is bound to the tip (surface) of dendrimer 3 which goes into a complementary combination with target DNA.
  • When a solution containing target DNA is poured into the biochip structured as described above and is made to flow through flow channel 2, the target DNA combines with the probe DNA in a complementary manner. Consequently, the target DNA is captured in a highly efficient manner.
  • It should be noted that the density of probe DNA molecules bound to the tips of dendrimer molecules has the optimum value, depending on the type of target (DNA, mRNA or protein). This optimum value can be obtained by changing the number of dendrimer layers (generations). Under normal conditions, second or higher generation dendrimers are used.
  • It is to be understood that the present invention is not restricted to the foregoing embodiments; rather, many other alterations and modifications thereof may be made without departing from the spirit and essential characteristics thereof. It is therefore intended that such alterations and modifications be covered by the appended claims.
  • For example, a plurality of concavities and convexities 4 may be formed on the walls of flow channel 2 so that turbulence takes place when a solution containing target DNA flows through flow channel 2, as illustrated in FIG. 2. This turbulence further increases the efficiency of the complementary combination between target DNA and probe DNA.
  • In the foregoing embodiments, an example has been shown wherein DNA segments are bound to the surfaces of dendrimer molecules and target and probe molecules are combined by means of hybridization. Alternatively, molecules of a biopolymer probe other than DNA may be bound so that target molecules combine with the probe molecules. Alternatively still, antibody molecules may be bound to the surfaces of dendrimer molecules as probe molecules so that the protein of target molecules is extracted by means of antigen-antibody reaction.
  • Alternatively still, it is possible to adopt the desired number and shape of flow channels 2 formed in substrate 1, without being restricted to those discussed in the foregoing embodiments.
  • As is evident from the above description, the following advantageous effects are achieved according to the present invention.
    • (1) By forming dendrimer molecules in a flow channel through which a biopolymer solution flows and binding molecules of a probe biopolymer or antibody to the tips of the dendrimer molecules, it is possible to easily and efficiently retrieve molecules of a target biopolymer or protein by means of a complementary combination or antigen-antibody reaction.
    • (2) While the conventional method using magnetic beads requires mechanical moving parts, the present invention eliminates the need for such mechanical moving parts, making it possible to easily miniaturize the preprocessing area where a target biopolymer or protein is retrieved.

Claims (7)

1. A dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed, is formed in the substrate of said biochip, a plurality of dendrimer molecules, one end of each of which is bound to the walls of said flow channel, are formed thereon, probe biopolymer or antibody molecules are bound to the tips of said dendrimer molecules and, if said probe biopolymer molecules are bound, then target biopolymer molecules are captured by means of a complementary combination and, if said antibody molecules are bound, then protein is extracted by means of antigen-antibody reaction.
2. The dendrimer-based biochip of claim 1, wherein said dendrimer is a multibranched polyamide amine dendrimer produced by providing aminosilane treatment on the walls of said flow channel and overlaying a film of amidoamine, which is produced by the reaction of methyl acrylate with ethylenediamine, upon the aminosilane-treated area as a dendron unit.
3. The dendrimer-based biochip of claim 1, wherein the generation of said dendrimer molecules is second or higher.
4. The dendrimer-based biochip of claim 2, wherein the generation of said dendrimer molecules is second or higher.
5. The dendrimer-based biochip of claim 1, wherein concavities and convexities are formed on the walls of said flow channel.
6. The dendrimer-based biochip of claim 2, wherein concavities and convexities are formed on the walls of said flow channel.
7. The dendrimer-based biochip of claim 3, wherein concavities and convexities are formed on the walls of said flow channel.
US11/501,900 2003-12-16 2006-08-10 Dendrimer-based DNA extraction methods and biochips Abandoned US20060269961A1 (en)

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Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110060136A1 (en) * 2009-09-09 2011-03-10 Tokyo University Of Agriculture And Technology Dendrimer-coated magnetic fine particles, and method for preparing same and utility thereof
CN103232363A (en) * 2013-04-18 2013-08-07 西安理工大学 Method for continuously preparing poly(amido-amine)
JP2013255517A (en) * 2007-07-13 2013-12-26 Handylab Inc Polynucleotide capture material and method for using the same
US9080207B2 (en) 2006-03-24 2015-07-14 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US9670528B2 (en) 2003-07-31 2017-06-06 Handylab, Inc. Processing particle-containing samples
US9677121B2 (en) 2001-03-28 2017-06-13 Handylab, Inc. Systems and methods for thermal actuation of microfluidic devices
US9765389B2 (en) 2011-04-15 2017-09-19 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
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US9815057B2 (en) 2006-11-14 2017-11-14 Handylab, Inc. Microfluidic cartridge and method of making same
US10179910B2 (en) 2007-07-13 2019-01-15 Handylab, Inc. Rack for sample tubes and reagent holders
US10364456B2 (en) 2004-05-03 2019-07-30 Handylab, Inc. Method for processing polynucleotide-containing samples
US10571935B2 (en) 2001-03-28 2020-02-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
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US10799862B2 (en) 2006-03-24 2020-10-13 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11453906B2 (en) 2011-11-04 2022-09-27 Handylab, Inc. Multiplexed diagnostic detection apparatus and methods
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system

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US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
EP1623953A4 (en) * 2003-04-25 2011-10-26 Nat Inst Inf & Comm Tech Probe
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JP2011055751A (en) * 2009-09-09 2011-03-24 Tokyo Univ Of Agriculture & Technology Method for preparing dendrimer-modified magnetic fine particle
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JP6778380B2 (en) 2016-08-23 2020-11-04 昭和電工マテリアルズ株式会社 Adsorbent
WO2019207669A1 (en) * 2018-04-25 2019-10-31 株式会社 日立ハイテクノロジーズ Substrate for nucleic acid analysis and flow cell for nucleic acid analysis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278048A (en) * 1988-10-21 1994-01-11 Molecular Devices Corporation Methods for detecting the effect of cell affecting agents on living cells
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
US20020182603A1 (en) * 2001-04-20 2002-12-05 Chapman William H. Uniformly functionalized surfaces for microarrays
US20030148367A1 (en) * 1997-08-29 2003-08-07 Olympus Optical Co., Ltd., A Japanese Corporation DNA capillary
US20030190608A1 (en) * 1999-11-12 2003-10-09 Gary Blackburn Microfluidic devices comprising biochannels
US20040171097A1 (en) * 2001-04-17 2004-09-02 Jens Schneider-Mergener Method for determining the substrate specificity of an enzymatic activity and a device therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08176212A (en) 1994-12-21 1996-07-09 Japan Synthetic Rubber Co Ltd Method of surface-modifying magnetic particle
JP4196459B2 (en) 1997-12-25 2008-12-17 東ソー株式会社 Magnetic carrier, method for producing the same, and method for extracting nucleic acid using the same
JP3756477B2 (en) * 2002-09-17 2006-03-15 横河電機株式会社 Method for extracting nucleic acid or protein with dendrimer and dendrimer composition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278048A (en) * 1988-10-21 1994-01-11 Molecular Devices Corporation Methods for detecting the effect of cell affecting agents on living cells
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
US20030148367A1 (en) * 1997-08-29 2003-08-07 Olympus Optical Co., Ltd., A Japanese Corporation DNA capillary
US20030190608A1 (en) * 1999-11-12 2003-10-09 Gary Blackburn Microfluidic devices comprising biochannels
US20040171097A1 (en) * 2001-04-17 2004-09-02 Jens Schneider-Mergener Method for determining the substrate specificity of an enzymatic activity and a device therefor
US20020182603A1 (en) * 2001-04-20 2002-12-05 Chapman William H. Uniformly functionalized surfaces for microarrays

Cited By (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
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US10571935B2 (en) 2001-03-28 2020-02-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US10351901B2 (en) 2001-03-28 2019-07-16 Handylab, Inc. Systems and methods for thermal actuation of microfluidic devices
US9677121B2 (en) 2001-03-28 2017-06-13 Handylab, Inc. Systems and methods for thermal actuation of microfluidic devices
US11078523B2 (en) 2003-07-31 2021-08-03 Handylab, Inc. Processing particle-containing samples
US10865437B2 (en) 2003-07-31 2020-12-15 Handylab, Inc. Processing particle-containing samples
US10731201B2 (en) 2003-07-31 2020-08-04 Handylab, Inc. Processing particle-containing samples
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US10604788B2 (en) 2004-05-03 2020-03-31 Handylab, Inc. System for processing polynucleotide-containing samples
US11441171B2 (en) 2004-05-03 2022-09-13 Handylab, Inc. Method for processing polynucleotide-containing samples
US10443088B1 (en) 2004-05-03 2019-10-15 Handylab, Inc. Method for processing polynucleotide-containing samples
US10364456B2 (en) 2004-05-03 2019-07-30 Handylab, Inc. Method for processing polynucleotide-containing samples
US10494663B1 (en) 2004-05-03 2019-12-03 Handylab, Inc. Method for processing polynucleotide-containing samples
US9802199B2 (en) 2006-03-24 2017-10-31 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
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US9080207B2 (en) 2006-03-24 2015-07-14 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10695764B2 (en) 2006-03-24 2020-06-30 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10710069B2 (en) 2006-11-14 2020-07-14 Handylab, Inc. Microfluidic valve and method of making same
US9815057B2 (en) 2006-11-14 2017-11-14 Handylab, Inc. Microfluidic cartridge and method of making same
US10179910B2 (en) 2007-07-13 2019-01-15 Handylab, Inc. Rack for sample tubes and reagent holders
JP2013255517A (en) * 2007-07-13 2013-12-26 Handylab Inc Polynucleotide capture material and method for using the same
US10590410B2 (en) 2007-07-13 2020-03-17 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
JP2015133995A (en) * 2007-07-13 2015-07-27 ハンディーラブ インコーポレイテッド Polynucleotide capture materials, and methods of using the same
US10625261B2 (en) 2007-07-13 2020-04-21 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10625262B2 (en) 2007-07-13 2020-04-21 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10632466B1 (en) 2007-07-13 2020-04-28 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10234474B2 (en) 2007-07-13 2019-03-19 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US10717085B2 (en) 2007-07-13 2020-07-21 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US10139012B2 (en) 2007-07-13 2018-11-27 Handylab, Inc. Integrated heater and magnetic separator
US11549959B2 (en) 2007-07-13 2023-01-10 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US11466263B2 (en) 2007-07-13 2022-10-11 Handylab, Inc. Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
JP2017060501A (en) * 2007-07-13 2017-03-30 ハンディーラブ インコーポレイテッド Polynucleotide capture materials, and methods of using the same
US10844368B2 (en) 2007-07-13 2020-11-24 Handylab, Inc. Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
US10071376B2 (en) 2007-07-13 2018-09-11 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10065185B2 (en) 2007-07-13 2018-09-04 Handylab, Inc. Microfluidic cartridge
US11266987B2 (en) 2007-07-13 2022-03-08 Handylab, Inc. Microfluidic cartridge
US11254927B2 (en) 2007-07-13 2022-02-22 Handylab, Inc. Polynucleotide capture materials, and systems using same
US10875022B2 (en) 2007-07-13 2020-12-29 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US11060082B2 (en) 2007-07-13 2021-07-13 Handy Lab, Inc. Polynucleotide capture materials, and systems using same
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US20110060136A1 (en) * 2009-09-09 2011-03-10 Tokyo University Of Agriculture And Technology Dendrimer-coated magnetic fine particles, and method for preparing same and utility thereof
US10781482B2 (en) 2011-04-15 2020-09-22 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US9765389B2 (en) 2011-04-15 2017-09-19 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
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US10076754B2 (en) 2011-09-30 2018-09-18 Becton, Dickinson And Company Unitized reagent strip
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US11453906B2 (en) 2011-11-04 2022-09-27 Handylab, Inc. Multiplexed diagnostic detection apparatus and methods
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
CN103232363A (en) * 2013-04-18 2013-08-07 西安理工大学 Method for continuously preparing poly(amido-amine)
CN111512158A (en) * 2017-12-22 2020-08-07 威斯康星校友研究基金会 Nano-engineered surfaces for cancer biomarker capture

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JP2005176613A (en) 2005-07-07
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