US20060160712A1 - Antimicrobial composition - Google Patents
Antimicrobial composition Download PDFInfo
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- US20060160712A1 US20060160712A1 US11/318,062 US31806205A US2006160712A1 US 20060160712 A1 US20060160712 A1 US 20060160712A1 US 31806205 A US31806205 A US 31806205A US 2006160712 A1 US2006160712 A1 US 2006160712A1
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D5/00—Processes for applying liquids or other fluent materials to surfaces to obtain special surface effects, finishes or structures
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D7/00—Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials
- B05D7/50—Multilayers
- B05D7/52—Two layers
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D7/00—Features of coating compositions, not provided for in group C09D5/00; Processes for incorporating ingredients in coating compositions
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0008—Detergent materials or soaps characterised by their shape or physical properties aqueous liquid non soap compositions
- C11D17/0017—Multi-phase liquid compositions
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2068—Ethers
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2075—Carboxylic acids-salts thereof
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/39—Organic or inorganic per-compounds
- C11D3/3945—Organic per-compounds
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/39—Organic or inorganic per-compounds
- C11D3/3947—Liquid compositions
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/43—Solvents
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/263—Ethers
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/265—Carboxylic acids or salts thereof
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/50—Solvents
- C11D7/5004—Organic solvents
- C11D7/5022—Organic solvents containing oxygen
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D1/00—Processes for applying liquids or other fluent materials
- B05D1/32—Processes for applying liquids or other fluent materials using means for protecting parts of a surface not to be coated, e.g. using stencils, resists
- B05D1/322—Removable films used as masks
- B05D1/325—Masking layer made of peelable film
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D3/00—Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials
- B05D3/06—Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials by exposure to radiation
- B05D3/061—Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials by exposure to radiation using U.V.
- B05D3/065—After-treatment
- B05D3/067—Curing or cross-linking the coating
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2006—Monohydric alcohols
- C11D3/2034—Monohydric alcohols aromatic
Definitions
- This invention relates to compositions that can be used, for example, to clean, reduce the microbial population of, or sterilize surfaces, and to compositions that can be used for aseptic packaging.
- antimicrobial agents having improved antimicrobial efficacy and improved speed of action.
- the specific requirements for such agents vary according to the intended application (e.g., sanitizer, disinfectant, sterilant, aseptic packaging treatment, etc.) and the applicable public health requirements.
- sanitizer should provide a 99.999% reduction (5-log order reduction) within 30 seconds at room temperature, 25 ⁇ 2° C., against several test organisms.
- antimicrobial agents e.g., iodophors, peracids, hypochlorites, chlorine dioxide, ozone, etc.
- these agents sometimes have inadequate activity against bacterial spores, fungal spores, and fungi. Killing, inactivating, or otherwise reducing the active population of bacterial spores and fungi on surfaces is particularly difficult.
- Bacterial spores have a unique chemical composition of spore layers that make them more resistant than vegetative bacteria to the antimicrobial effects of chemical and physical agents.
- the unique chemical composition of fungal cells, especially mold spores makes them more resistant to chemical and physical agents than are other microorganisms. This resistance can be particularly troublesome when the spores or fungi are located on surfaces such as food, food contact sites, ware, hospitals and veterinary facilities, surgical implements, and hospital and surgical linens and garments.
- Control of the mold Chaetomium funicola , and of bacterial spore-forming microorganisms of the Bacillus species, can be especially important during food packaging, particularly during cold or hot aseptic filling of food and beverage products.
- Microorganisms of the Bacillus species include Bacillus cereus, Bacillus mycoides, Bacillus subtilis, Bacillus anthracis , and Bacillus thuringiensis . These latter microorganisms share many phenotypical properties, have a high level of chromosomal sequence similarity, and are known enterotoxin producers.
- Bacillus cereus is one of the most problematic because Bacillus cereus has been identified as possessing increased resistance to germicidal chemicals used to decontaminate environmental surfaces.
- Bacillus cereus is frequently diagnosed as a cause of gastrointestinal disorders and has been suggested to be the cause of several food-borne illness outbreaks. Due to its rapid sporulating capacity, Bacillus cereus easily survives in the environment. Bacillus cereus is omnipresent in nature, and consequently can usually be found in animal feed and fodder. Bacillus cereus can contaminate raw milk via feces and soil, and can survive intestinal passage in cows and the pasteurization process.
- Bacillus cereus is also known to cause serious human illness via environmental contamination.
- Bacillus cereus is known to cause post-traumatic injury eye infections, which can result in visual impairment or loss of vision within 12-48 hours after infection.
- Bacillus cereus is regarded as transferable from washed surgical garments to patients.
- Agents having greater or faster activity against bacterial spores, fungi, and other resistant microorganisms could help meet a substantial public health need, and one that is not adequately addressed by current commonly-used antimicrobial agents.
- the present invention provides, in one aspect, a method for antimicrobial treatment comprising applying to microbes a composition containing a diluting solvent (e.g., water), an antimicrobially-active solvent having a density different from the density of the diluting solvent, and an optional cosolvent, surfactant, or additional antimicrobial agent, wherein the amount of antimicrobially-active solvent or additional antimicrobial agent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus within 10 seconds at 60° C.
- a diluting solvent e.g., water
- the methods of the invention provide broader spectrum antimicrobial action, providing greater than a 1-log order reduction within 10 seconds at 60° C. in one or more additional organisms such as the mold Chaetomium funicola . In a more preferred aspect, the methods of the invention provide greater than a 1-log order reduction within 10 seconds at 60° C. in Chaetomium funicola, Bacillus subtilis and Bacillus cereus.
- the invention provides a method for antimicrobial treatment, comprising applying to microbes a composition as described above, wherein the composition further comprises such additional antimicrobial agent.
- the additional antimicrobial agent comprises a peracid such as peroxyacetic acid; a peroxide such as hydrogen peroxide; or a halogen containing compound such as hypochlorous acid (or its salts), chlorine dioxide, hypobromous acid (or its salts), or an interhalide such as iodine monochloride, iodine dichloride, iodine tetrachloride, bromine chloride, iodine monobromide, or iodine dibromide.
- the invention provides an antimicrobial concentrate and instructions for mixing the concentrate with water, wherein the concentrate comprises an antimicrobially-active solvent that has a density different from that of water, an optional cosolvent or surfactant, and an optional additional antimicrobial agent, the amounts of antimicrobially-active solvent and optional additional antimicrobial agent being sufficiently high and the amount of cosolvent or surfactant being sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus within 10 seconds at 60° C.
- the composition comprises said additional antimicrobial agent and the amount of antimicrobially-active solvent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition does not form a clear single-phase solution or microemulsion when the concentrate is mixed with water according to the instructions.
- the invention provides an antimicrobial composition
- a diluting solvent comprising a diluting solvent, an antimicrobially-active solvent having a density that is different from the density of the diluting solvent, an additional antimicrobial agent, and an optional cosolvent or surfactant, the amounts of antimicrobially-active solvent and of additional antimicrobial agent being sufficiently high and the amount of cosolvent or surfactant being sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus or the mold Chaetomium funicola within 10 seconds at 60° C.
- the invention provides an antimicrobial concentrate and instructions for mixing the concentrate with water, wherein the concentrate comprises an antimicrobially-active solvent that has a density different from that of water, an optional cosolvent or surfactant, and an additional antimicrobial agent, the amounts of antimicrobially-active solvent and additional antimicrobial agent being sufficiently high so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus or the mold Chaetomium funicola within 10 seconds at 60° C.
- the composition comprises a sufficiently high amount of additional antimicrobial agent and antimicrobially-active solvent such that the composition forms a clear single-phase solution when the concentrate is mixed with water according to the instructions, and provides greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus or Bacillus subtilis and in the population of the mold Chaetomium funicola within 10 seconds at 60° C.
- compositions of the invention are especially useful for aseptic packaging, re-use clean-in-place (CIP) or clean-out-of-place (COP) systems, hospital disinfectants, veterinary clinic disinfectants, and as sporicides or sterilants.
- CIP clean-in-place
- COP clean-out-of-place
- the term “sterilant” refers to a physical or chemical agent or process capable of destroying all forms of life (including bacteria, viruses, fungi, and spores) on inanimate surfaces.
- a physical or chemical agent or process capable of destroying all forms of life (including bacteria, viruses, fungi, and spores) on inanimate surfaces.
- One procedure is described in A.O.A.C. Sporicidal Activity of Disinfectants , Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 966.04 and applicable sections, 15 th Edition, 1990 (EPA Guideline 91-2)
- the term “antimicrobial composition” refers to a composition having the ability to cause greater than a 90% reduction (1-log order reduction) in the population of bacteria or spores of Bacillus species within 10 seconds at 60° C., using the above-mentioned Germicidal and Detergent Sanitizing Action of Disinfectants procedure.
- Bacillus cereus or Bacillus subtilis are used in such procedure.
- the antimicrobial compositions of the invention provide greater than a 99% reduction (2-log order reduction), more preferably greater than a 99.99% reduction (4-log order reduction), and most preferably greater than a 99.999% reduction (5-log order reduction) in such population within 10 seconds at 60° C.
- the antimicrobial compositions of the invention also provide greater than a 99% reduction (2-log order reduction), more preferably greater than a 99.99% reduction (4-log order reduction), and most preferably greater than a 99.999% reduction (5-log order reduction) in the population of one or more additional organisms such as the mold Chaetomium funicola . Because in their broadest sense these definitions for antimicrobial activity are different from some of the current governmental regulations, the use in connection with this invention of the term “antimicrobial” is not intended to indicate compliance with any particular governmental standard for antimicrobial activity.
- the term “sporicide” refers to a physical or chemical agent or process having the ability to cause greater than a 90% reduction (1-log order reduction) in the population of spores of Bacillus cereus or Bacillus subtilis within 10 seconds at 60° C.
- the sporicidal compositions of the invention provide greater than a 99% reduction (2-log order reduction), more preferably greater than a 99.99% reduction (4-log order reduction), and most preferably greater than a 99.999% reduction (5-log order reduction) in such population within 10 seconds at 60° C.
- sanitizer refers to an agent that reduces the number of bacterial contaminants to safe levels as judged by public health requirements.
- sanitizers for use in this invention will provide at least a 99.999% reduction (5-log order reduction) using the Germicidal and Detergent Sanitizing Action of Disinfectants procedure referred to above.
- the term “disinfectant” refers to an agent that kills all vegetative cells including most recognized pathogenic microorganisms, using the procedure described in A.O.A.C. Use Dilution Methods , Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 955.14 and applicable sections, 15th Edition, 1990 (EPA Guideline 91-2).
- preservative refers to an agent that extends the storage life of food and non-food products by retarding or preventing deterioration of flavor, odor, color, texture, appearance, nutritive value, or safety.
- a preservative need not provide a lethal, irreversible action resulting in partial or complete microbial cell destruction or incapacitation.
- Sterilants, sanitizers, disinfectants, sporicides, viracides and tuberculocidal agents provide such an irreversible mode of action, sometimes referred to as “bactericidal” action.
- a preservative can provide an inhibitory or bacteriostatic action that is reversible, in that the target microbes can resume multiplication if the preservative is removed.
- the principal differences between a preservative and a sanitizer primarily involve mode of action (a preservative prevents growth rather than killing microorganisms) and exposure time (a preservative has days to months to act whereas a sanitizer has at most a few minutes to act).
- compositions of the invention When applied to microbes (e.g., when applied to a surface containing microbes), the compositions of the invention exhibit antimicrobial action.
- the mechanism by which such action takes place is not completely understood. However, as shown in the Examples set out below, very rapid and substantially complete antimicrobial action can be attained.
- compositions and methods of the invention provide “pseudo-stable” antimicrobial compositions that phase-separate following application of the composition to a surface. These compositions can also be described as exhibiting “phase-splitting” characteristics.
- phase refers to a homogeneous fluid portion that is present in or that can form in a fluid system.
- phases refers to the presence of more than one phase in a heterogeneous fluid system.
- phase-splitting is meant to describe a single phase antimicrobially-active solvent-containing composition that forms at least two laminar phases promptly after being applied atop a generally horizontal surface or on a generally vertical surface, whereby a film containing a concentrated amount of the antimicrobially-active solvent lies between the surface and a film containing a much lower amount of the antimicrobially-active solvent.
- the phase containing a concentrated amount of the antimicrobially-active solvent will be referred to as the solvent phase
- the phase containing a much lower amount of the antimicrobially-active solvent will be referred to as the dilute phase or diluting phase.
- the solvent phase will lie atop the surface (or atop microbes on the surface) and under the dilute phase or phases.
- the solvent phase will lie adjacent the surface (or adjacent microbes on the surface) and under the dilute phase or phases.
- attainment of pseudo-stable phase-splitting behavior can be achieved by employing a sufficiently high amount of antimicrobially-active solvent and a sufficiently low amount of cosolvent or surfactant.
- the amount of antimicrobially-active solvent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition forms a “quasi-stable” antimicrobial composition.
- Such compositions have a clear or slightly cloudy appearance, do not form a clear single-phase solution or microemulsion, and do not undergo phase-splitting.
- they are antimicrobial compositions as defined herein. If in such quasi-stable compositions the amount of antimicrobially-active solvent is increased sufficiently, or if the amount of cosolvent or surfactant is decreased sufficiently, then these compositions will become pseudo-stable.
- these quasi-stable compositions almost exhibit pseudo-stable behavior, and will do so if modified as taught herein. As shown in some of the Examples set out below, these quasi-stable compositions can provide significant antimicrobial activity even though they do not undergo phase-splitting during use.
- compositions that upon standing will form clear one-phase mixtures, cloudy two-phase dispersions or phase-splitting two-phase mixtures, it being understood that compositions forming three or more phases upon standing could be employed if desired.
- compositions of the invention can be formulated and sold for use as is, or as solvent concentrates. If desired, such concentrates can be used full-strength as antimicrobial agents. However, the concentrates typically will be diluted with a fluid (e.g., water) that subsequently forms the dilute phase. Preferably, the concentrate forms a single phase before such dilution and remains so while stored in the container in which it will be sold.
- a fluid e.g., water
- mild agitation e.g., by stirring or pumping the composition
- some compositions of the invention will form a pseudo-stable dispersion, and other compositions of the invention will form a clear or quasi-stable solution or dispersion.
- the composition preferably remains in the pseudo-stable state for a sufficiently long period so that the composition can be applied to a surface before the onset of phase separation.
- the pseudo-stable state need only last for a few seconds when suitably rapid application techniques such as spraying are employed, or when agitation during application is employed.
- the pseudo-stable state desirably lasts for at least one minute or more after mixing and while the composition is stored in a suitable vessel, and preferably lasts for five minutes or more after mixing. Often normal refilling or replenishment of the applicator (e.g., by dipping the applicator in the composition) will provide sufficient agitation to preserve the pseudo-stable state of the composition during application.
- a variety of fluids can be used as the diluting solvent, including water in its liquid form; steam; condensed gases and other supercritical fluids (e.g., CO 2 ); perchloroethylene; oils such as silicone oils (e.g., siloxanes), gear oils, transaxle oils, mineral oils or vegetable oils; and carboxylic esters such as methyl soyate.
- oils such as silicone oils (e.g., siloxanes), gear oils, transaxle oils, mineral oils or vegetable oils; and carboxylic esters such as methyl soyate.
- oils include food grade or food-derived oils, flavorings, or fragrance oils.
- the diluting solvent consists essentially of or consists of water in its liquid form. The remainder of this specification will primarily discuss the use of water in its liquid form as the diluting solvent, it being understood that other suitable fluids could be added to or substituted for water in its liquid form if desired.
- compositions of the invention can contain a variety of antimicrobially-active solvents.
- the antimicrobially-active solvent preferably is insoluble, or only sparingly soluble, in the diluting solvent.
- the antimicrobially-active solvent preferably will have a water solubility less than about 5 wt. %, more preferably less than about 3 wt. %, and most preferably less than about 2 wt. %.
- the antimicrobially-active solvent is selected based upon the characteristics of the surface and microbes to which the antimicrobial composition will be applied and upon the nature of any coating, soil or other material that will be contacted by the antimicrobial composition and optionally removed from the surface.
- Polar solvents, and solvents that are capable of hydrogen bonding typically will perform well on a variety of surfaces and microbes and thus are preferred.
- the antimicrobially-active solvent also has a high flashpoint (e.g., greater than about 30° C., more preferably greater than about 50° C., and most preferably greater than about 100° C.), low odor and low human and animal toxicity.
- the antimicrobially-active solvent is a food-grade or cosmetic or flavorant additive.
- Preferred antimicrobially-active solvents having a density different from that of water include acetamidophenol (specific gravity 1.027); acetanilide (specific gravity 1.219; water solubility ⁇ 1%); acetophenone (specific gravity 1.0238; water solubility ⁇ 1%); [2-acetyl-1-methylpyrrole (specific gravity 1.04); benzyl acetate (specific gravity 1.0515; water solubility ⁇ 1%); benzyl alcohol (specific gravity 1.0413; water solubility ⁇ 4%); benzyl benzoate (specific gravity 1.118; water solubility ⁇ 1%); benzyloxyethanol (specific gravity 1.07; water solubility ⁇ 1%); ethers or hydroxyethers such as ethylene glycol phenyl ether (specific gravity 1.104; water solubility 2.3%; commercially available as DOWANOL EPHTM from Dow Chemical Co.) and propylene glycol pheny
- the water solubilities noted above are room temperature values.
- Benzyl alcohol, phenylethanol, essential oils, dibasic esters, dialkyl carbonates, ethylene glycol phenyl ether and propylene glycol phenyl ether are particularly preferred antimicrobially-active solvents. Mixtures of antimicrobially-active solvents can be used if desired.
- compositions of the invention should contain sufficient antimicrobially-active solvent to provide the desired rate and type of microbial reduction.
- antimicrobial concentrates of the invention will contain at least about 5 wt. % antimicrobially-active solvent, preferably at least about 25 wt. % antimicrobially-active solvent, more preferably at least about 65 wt. % antimicrobially-active solvent, and most preferably about 75 to about 95 wt. % antimicrobially-active solvent.
- compositions of the invention can contain one or more cosolvents or surfactants to assist in providing pseudo-stable or quasi-stable behavior.
- cosolvents or surfactants that are present at concentrations below those at which single-phase coupling arises, or cosolvents or surfactants that are relatively inefficient or ineffective (with respect to their ability completely to solubilize or disperse the antimicrobially-active solvent in the diluting solvent and form a single-phase system), are preferred over cosolvents or surfactants that are present at higher concentrations or are more efficient or effective. This differs from the approach normally taken when formulating compositions containing cosolvents or surfactants. Normally, cosolvents and surfactants are selected for their ability to promote formation of stable single-phase solutions, microemulsions, or dispersions.
- cosolvents can be employed.
- the cosolvent is selected based upon the characteristics of the chosen antimicrobially-active solvent and the solubility of the chosen antimicrobially-active solvent in the diluting solvent.
- the cosolvent generally will have higher water solubility than the water solubility of the chosen solvent.
- the cosolvent has a high flashpoint (e.g., greater than about 30° C., more preferably greater than about 50° C., and most preferably greater than about 100° C.), low odor and low human and animal toxicity.
- Preferred cosolvents include 2-(2-aminoethoxy)ethanol, monoethanolamine, diethanolamine, triethanolamine, amyl acetate, amyl alcohol, butanol, 3-butoxyethyl-2-propanol, butyl acetate, n-butyl propionate, cyclohexanone, diacetone alcohol, diethoxyethanol, diethylene glycol methyl ether, diethylene glycol n-butyl ether, diisobutyl carbinol, diisobutyl ketone, dimethyl heptanol, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene glycol propyl ether, dipropylene glycol tert-butyl ether, ethanol, ethyl acetate, 2-ethylhexanol, ethyl propionate, ethylene glycol butyl ether, ethylene glycol methyl ether
- cosolvents include Butoxyethyl PROPASOLTM, Butyl CARBITOLTM acetate, Butyl CARBITOLTM, Butyl CELLOSOLVETM acetate, Butyl CELLOSOLVETM, Butyl DIPROPASOLTM, Butyl PROPASOLTM, CARBITOLTMPM-600, CARBITOLTM Low Gravity, CELLOSOLVETM acetate, CELLOSOLVETM, Ester EEPTM, FILMER IBTTM, Hexyl CARBITOLTM, Hexyl CELLOSOLVETM, Methyl CARBITOLTM, Methyl CELLOSOLVETM acetate, Methyl CELLOSOLVETM, Methyl DIPROPASOLTM, Methyl PROPASOLTM acetate, Methyl PROPASOLTM, Propyl CARBITOLTM, Propyl CELLOSOLVETM, Propyl CARBITOLTM, Propyl CELLOSOLVE
- compositions of the invention preferably should not contain excessive cosolvent, as the use of an excess of cosolvent will tend to cause formation of an antimicrobially inert single-phase solution or microemulsion.
- the amount of cosolvent preferably is just sufficient to provide the desired level of antimicrobial activity. Larger amounts of cosolvent may diminish the antimicrobial effectiveness of the compositions of the invention.
- antimicrobial concentrates of the invention will contain 0 to about 50 wt. % cosolvent, more preferably 0 to about 25 wt. % cosolvent, and most preferably 0 to about 20 wt. % cosolvent.
- surfactants can be employed.
- the surfactant identity and use level is selected based upon the characteristics of the chosen antimicrobially-active solvent and the solubility of the chosen antimicrobially-active solvent in the diluting solvent.
- the surfactant preferably will have an HLB value greater than or equal to about 13, or less than or equal to about 6. This value reflects the above-noted preference in the present invention for employing surfactants that are relatively inefficient or ineffective as emulsifiers.
- the surfactant does not tend to cause formation of insoluble deposits, and has low odor and low toxicity. Mixtures of surfactants can be used if desired.
- Preferred anionic surfactants include C 6 -C 24 alkylbenzene sulfonates; C 6 -C 24 olefin sulfonates; C 6 -C 24 paraffin sulfonates; cumene sulfonate; xylene sulfonate; C 6 -C 24 alkyl naphthalene sulfonates; C 6 -C 24 alkyl or dialkyl diphenyl ether sulfonates or disulfonates, C 4 -C 24 mono or dialkyl sulfosuccinates; sulfonated or sulfated fatty acids; C 6 -C 24 alcohol sulfates (preferably C 6 -C 12 alcohol sulfates); C 6 -C 24 alcohol ether sulfates having 1 to about 20 ethylene oxide groups; and C 4 -C 24 alkyl, aryl or alkaryl phosphate esters or their alkoxylated an
- Preferred nonionic surfactants include C 6 -C 24 alcohol ethoxylates (preferably C 6 -C 14 alcohol ethoxylates) having 1 to about 20 ethylene oxide groups (preferably about 9 to about 20 ethylene oxide groups); C 6 -C 24 alkylphenol ethoxylates (preferably C 8 -C 10 alkylphenol ethoxylates) having 1 to about 100 ethylene oxide groups (preferably about 12 to about 20 ethylene oxide groups); C 6 -C 24 alkylpolyglycosides (preferably C 6 -C 20 alkylpolyglycosides) having 1 to about 20 glycoside groups (preferably about 9 to about 20 glycoside groups); C 6 -C 24 fatty acid ester ethoxylates, propoxylates or glycerides; and C4-C 24 mono or di alkanolamides.
- C 6 -C 24 alcohol ethoxylates preferably C 6 -C 14 alcohol ethoxylates
- Preferred cationic surfactants include quaternary amine compounds having the formula:
- R, R′, R′′ and R′′′ are each a C 1 -C 24 alkyl, aryl or aralkyl group that can optionally contain one or more P, O, S or N heteroatoms, and X is F, Cl, Br, I or an alkyl sulfate.
- Preferred amphoteric surfactants include amine oxide compounds having the formula:
- R, R′, R′′ and R′′′ are each a C 1 -C 24 alkyl, aryl or aralkyl group that can optionally contain one or more P, O, S or N heteroatoms.
- amphoteric surfactants includes betaine compounds having the formula:
- R, R′, R′′ and R′′′ are each a C 1 -C 24 alkyl, aryl or aralkyl group that can optionally contain one or more P, O, S or N heteroatoms, and n is about 1 to about 10.
- the antimicrobial compositions of the invention should not contain excessive amounts of surfactant, lest an antimicrobially inactive single-phase solution or microemulsion be formed. Instead, the amount of surfactant should be just sufficient to provide the desired level of antimicrobial activity. Larger amounts of surfactant may diminish the antimicrobial effectiveness of the compositions of the invention.
- the solvent concentrates of the invention will contain no more than about 10 wt. % surfactant, more preferably 0 to about 3 wt. % surfactant and most preferably 0 to about 1 wt. % surfactant. Most preferably, the concentrates are substantially surfactant-free.
- the antimicrobial compositions of the invention preferably contain an additional antimicrobial agent.
- This additional antimicrobial agent can be dissolved or dispersed in the antimicrobially-active solvent or in the diluting solvent.
- the additional antimicrobial agent will preferentially dissolve or disperse in the antimicrobially-active solvent rather than in the diluting solvent.
- Suitable additional antimicrobial agents include carboxylic acids, diacids, or triacids (e.g., butyric acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, salycic acid, mandelic acid, succinic acid, adipic acid, glutaric acid, EDTA and citric acid), carboxylic esters (e.g., p-hydroxy alkyl benzoates and alkyl cinnamates), sulfonic acids (e.g., dodecylbenzene sulfonic acid), iodo-compounds or active halogen compounds (e.g., iodine, interhalides, polyhalides, metal hypochlorites, hypochlorous acid, metal hypbromites, hypobromous acid, chloro- and bromo-hydantoins, chlorine dioxide and sodium chlorite), active oxygen compounds including hydrogen peroxide, isolated or equilibrium
- additional antimicrobial agents having about 1-6 carbons, or an ionic charge, would be mostly soluble in the diluting solvent; those with higher carbon numbers would generally be more soluble in the antimicrobially-active solvent. In either case, for a pseudo-stable antimicrobial composition it is preferred to use additional antimicrobial agents that can be drawn into the solvent phase or onto surfaces during phase separation.
- compositions of the invention containing such optional additional antimicrobial agents appear to have substantially greater antimicrobial effectiveness than comparison aqueous solutions or dispersions containing the additional antimicrobial agent alone.
- the additional antimicrobial agent preferably is about 0.01 to about 30 wt. % of the concentrate, more preferably about 0.05 to about 10 wt. % and most preferably about 0.1 to about 5 wt. %.
- the antimicrobial compositions of the invention can contain various adjuvants such as chelants, builders, thickeners, fragrances, dyes, pH adjusters, anticorrosion additives, antirust additives and indicators.
- adjuvants such as chelants, builders, thickeners, fragrances, dyes, pH adjusters, anticorrosion additives, antirust additives and indicators.
- the types and amounts of such adjuvants will be apparent to those skilled in the art.
- compositions of the invention can be formulated to include the diluting solvent (e.g., water) as sold, or the diluting solvent can be added at any time up to the time of use.
- the concentrates of the invention contain little or no diluting solvent as sold.
- a variety of dilution ratios can be employed, so long as the diluted composition exhibits the desired antimicrobial behavior when applied to the target microbes.
- the ingredients in the concentrate can represent about 1 to about 99 wt. % of the diluted mixture, more preferably about 5 to about 50 wt. %, and most preferably about 6 to about 25 wt. %.
- the diluted antimicrobial compositions preferably contain about 0.01 to about 50 wt.
- the diluted composition preferably contains antimicrobially-active solvent in an amount near the solubility limit of the antimicrobially-active solvent in the diluting solvent.
- the diluted antimicrobial compositions preferably are aqueous, contain additional antimicrobial agent, and are clear or quasi-stable.
- compositions of the invention can be sold in the form of a kit containing the composition together with suitable directions for carrying out the method of the invention.
- suitable directions typically will include recommended dilution ratios, applications, application techniques and safety warnings.
- Fuller Formula 3100TM Super Concentrate (Fuller Brush, Québec)
- Fuller Formula 3100TM Super Concentrate is believed to have contained about 49 wt. % benzyl alcohol, 17 wt. % monoethanolamine, 10 wt. % sodium decyldiphenyl ether disulfonate and 24 wt. % water. Dilution of the concentrate at a 1:20 concentrate:water ratio was recommended on the product instructions.
- the resulting mixture formed a stable single-phase solution.
- the resulting mixture forms a quasi-stable or pseudo-stable composition.
- the composition is pseudo-stable and will undergo phase splitting when applied to a substrate and allowed to stand for a few minutes.
- the antimicrobial compositions of the invention can be used for a variety of domestic or industrial applications, e.g., to reduce microbial or viral populations on a surface or object or in a body or stream of water.
- the compositions can be applied in a variety of areas including kitchens, bathrooms, factories, hospitals, dental offices and food plants, and can be applied to a variety of hard or soft surfaces having smooth, irregular or porous topography.
- Suitable hard surfaces include, for example, architectural surfaces (e.g., floors, walls, windows, sinks, tables, counters and signs); eating utensils; hard-surface medical or surgical instruments and devices; and hard-surface packaging.
- Such hard surfaces can be made from a variety of materials comprising, for example, ceramic, metal, glass, wood or hard plastic.
- Suitable soft surfaces include, for example paper; filter media, hospital and surgical linens and garments; soft-surface medical or surgical instruments and devices; and soft-surface packaging.
- Such soft surfaces can be made from a variety of materials comprising, for example, paper, fiber, woven or nonwoven fabric, soft plastics and elastomers.
- the compositions of the invention can also be applied to soft surfaces such as food and skin.
- the compositions are also suitable for application to growing or harvested plant material including leaves, stems, tubers, roots, seeds, and the like.
- the antimicrobial compositions of the invention can be included in products such as sterilants, sanitizers, disinfectants, preservatives, deodorizers, antiseptics, fungicides, germicides, sporicides, virucides, detergents, bleaches, hard surface cleaners, hand soaps and pre- or post-surgical scrubs.
- the compositions have particular utility as cold or hot aseptic packaging treatments.
- the antimicrobial compositions can also be used in veterinary products such as mammalian skin treatments or in products for sanitizing or disinfecting animal enclosures, pens, watering stations, and veterinary treatment areas such as inspection tables and operation rooms.
- the antimicrobial compositions of the invention can be used for treating skin diseases on animals (especially mammals), or those which spread via transfer to air or surface substrates, such as disease from fungi, molds, bacteria spores and viruses.
- These spreadable skin diseases include athletes foot fungus and hairy hoof wart disease, and the many organisms leading to Mastitis and other mammalian milking diseases.
- the disease can be a viral disease such as parvovirus, coxsackie virus, or herpes virus.
- the disease can also be bacterial, such as S. aureus, E. coli, Streptococci, etc., or a Mycobacterium type such as that leading to tuberculosis.
- the compositions may also be used to treat animal carcasses to reduce both pathogenic and non-pathogenic microbial levels.
- the antimicrobial compositions can also be used on foods and plant species to reduce surface microbial populations; used at manufacturing or processing sites handling such foods and plant species; or used to treat process waters around such sites.
- the compositions can be used on food transport lines (e.g., as belt sprays); boot and hand-wash dip-pans; food storage facilities; anti-spoilage air circulation systems; refrigeration and cooler equipment; beverage chillers and warmers, blanchers, cutting boards, third sink areas, and meat chillers or scalding devices.
- the compositions of the invention can be used to treat produce transport waters such as those found in flumes, pipe transports, cutters, slicers, blanchers, retort systems, washers, and the like.
- the antimicrobial compositions have particular value for use on food packaging materials and equipment, and especially for cold or hot aseptic packaging.
- the compositions can also be used on or in ware wash machines, dishware, bottle washers, bottle chillers, warmers, third sink washers, cutting areas (e.g., water knives, slicers, cutters and saws) and egg washers.
- Particular foodstuffs that can be treated with compositions of the invention include eggs, meats, seeds, leaves, fruits and vegetables.
- Particular plant surfaces include both harvested and growing leaves, roots, seeds, skins or shells, stems, stalks, tubers, corms, fruit, and the like.
- Particular treatable surfaces include packaging such as cartons, bottles, films and resins; dish ware such as glasses, plates, utensils, pots and pans; ware wash machines; exposed food preparation area surfaces such as sinks, counters, tables, floors and walls; processing equipment such as tanks, vats, lines, pumps and hoses (e.g., dairy processing equipment for processing milk, cheese, ice cream and other dairy products); and transportation vehicles.
- packaging such as cartons, bottles, films and resins
- dish ware such as glasses, plates, utensils, pots and pans
- ware wash machines such as sinks, counters, tables, floors and walls
- processing equipment such as tanks, vats, lines, pumps and hoses (e.g., dairy processing equipment for processing milk, cheese, ice cream and other dairy products); and transportation vehicles.
- the antimicrobial compositions can also be used on or in other industrial equipment and in other industrial process streams such as heaters, cooling towers, boilers, retort waters, rinse waters, aseptic packaging wash waters, and the like.
- the compositions can be used to treat microbes and odors in recreational waters such as in pools, spas, recreational flumes and water slides, fountains, and the like.
- the antimicrobial compositions can also be used to reduce microbial and viral counts in air and liquids by incorporation into filtering media or breathing filters, e.g., to remove water and air-born pathogens such as Legionella.
- CIP clean-in-place systems
- COP clean-out-of-place systems
- washer-decontaminators sterilizers
- textile laundry machines ultra and nano-filtration systems and indoor air filters
- COP systems can include readily accessible systems including wash tanks, soaking vessels, mop buckets, holding tanks, scrub sinks, vehicle parts washers, non-continuous batch washers and systems, and the like.
- CIP systems include a variety of devices that will be familiar to those skilled in the art, and will typically employ flow rates on the order of about 40 to about 600 liters per minute, temperatures from ambient up to about 70° C., and contact times of at least about 10 seconds, more preferably about 30 to about 120 seconds.
- the antimicrobial compositions can be applied to microbes or to soiled or cleaned surfaces using a variety of methods.
- the antimicrobial composition can be sprayed or wiped onto a surface; the composition can be caused to flow over the surface, or the surface can be dipped into the composition.
- the compositions can be formulated as liquids, gels, aerosols, waxes, solids, or powders. If steam or another gaseous diluting solvent is employed, then the compositions can be formulated to be applied in a gaseous state.
- compositions were evaluated by comparing them against a commercially available aseptic bottle washing biocide based on mixed peracids (MATRIXXTM; Ecolab). Compositions containing only 1000 ppm or 2000 ppm of a single peracid or mixed peracids were used as controls. The remaining compositions were prepared by adding 10% of various solvents to an aqueous solution containing 1000 ppm or 2000 ppm of the mixed peracids. Non-solubilizing amounts of anionic surfactants were added to some of the compositions to affect minimal coupling and to yield, in some cases, pseudo-stable behavior and at least a partial phase-splitting condition. Addition of such non-stabilizing amounts tended to provide partial coupling and improved antimicrobial solution stability but not necessarily improved microbial control.
- compositions and controls were evaluated for antimicrobial activity using the procedure set out in set out in Germicidal and Detergent Sanitizing Action of Disinfectants , Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 960.09 and applicable sections, 15th Edition, 1990 (EPA Guideline 91-2), using a 10 second contact time at 60° C. against the mold Chaetomium funicola ( C. funicola ). This brief contact time presented an especially challenging test, as evidenced by low observed log order reduction values for the controls.
- compositions of the present invention were evaluated for biocidal control, using the method of Example 1, and compared to several commercial products and to formulations from several U.S. patents.
- the comparison compositions formed clear (single-phase) formulations when prepared according to instructions.
- the compositions of the invention formed pseudo-stable cloudy compositions that underwent phase splitting following application. All tested compositions were evaluated against the spore-forming, enterotoxin producing pathogens Bacillus cereus and Bacillus subtilis and the mold C. funicola using a 10 second contact time at 60° C. Set out below in Table II are the run number, benzyl alcohol amount, amounts of additional ingredients, appearance of the mixtures, after they had been allowed to stand for one minute, and observed log order reduction for B.
- compositions of the present invention in Run Nos. 2-10 through 2-19 yielded phase-splitting formulations that formed at least 2 phases.
- Run No. 2-20 yielded a pseudo-stable solution that was just slightly opaque but did not separate during the test time.
- the compositions of the invention exhibited significant antimicrobial efficacy against B. cereus , as well as broad-spectrum efficacy against B. subtilis and C. funicola .
- Example 1 Using the method of Example 1, 5% portions of various sparingly soluble solvents were added to plain water or to commercial peracid bottle washing formulas (KX-6091, 15C, or VORTEXXTM; Ecolab) and tested against the mold C. funicola using a 10 second contact time at 60° C. A non-emulsifying amount of the anionic surfactant sodium octene sulfonate was added to some of the compositions to slow down, but not prevent, phase-splitting. Set out below in Table III are the run number, solvent, peracid, peracid concentration, surfactant concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and observed log order reduction for C. funicola for each composition.
- Run Nos. 3-3 to 3-18 exhibited phase-splitting.
- Table III demonstrate that substantial improvements in antimicrobial efficacy could be obtained by modifying all three commercial aseptic wash products, as can be seen by comparing control Run No. 3-1 with Run Nos. 3-4 through 3-7, 3-9 through 3-13 and 3-15; control Run No. 3-2 with Run Nos. 3-16 and 3-17; and control Run No. 3-3 with Run No. 3-18.
- Run Nos. 3-8 and 3-14 exhibited significant antimicrobial efficacy without an additional antimicrobial agent.
- a composition containing both a sparingly soluble antimicrobially-active solvent and an additional antimicrobial agent exhibited a synergistic improvement in performance compared to the use of either the antimicrobially-active solvent or the additional antimicrobial agent alone, as can be seen by comparing Run No. 3-10 with Run Nos. 3-1 and 3-8.
- Example 2 Using the method of Example 2, varying amounts of several sparingly soluble solvents were added to commercial peracid bottle washing formulations (TSUNAMI-100TM, MATRIXXTM, or KX-6091; Ecolab) and tested against the mold C. funicola using a 10 second contact time at 60° C.
- the surfactant dodecylbenzene sulfonate (“DBS”) was added to some of the compositions to slow down, but not inhibit, phase-splitting.
- DBS dodecylbenzene sulfonate
- Table IV Set out below in Table IV are the run number, solvent, solvent concentration, peracid concentration, DBS concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and observed log order reduction for C. funicola for each composition. TABLE IV Run Solvent Peracid DBS C.
- Example 2 Using the method of Example 2, varying amounts of benzyl alcohol were added to commercial peracid bottle washing formulations (KX-6091, 15C, TSUNAMI-100TM, and VORTEXXTM; Ecolab) and tested against the spore-forming, enterotoxin producing pathogen Bacillus cereus and the mold C. funicola using a 10 second contact time at 60° C.
- Table V Set out below in Table V are the run number, solvent, solvent concentration, peracid concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and the observed log order reduction for Bacillus cereus and C. funicola for each composition.
- TABLE V Run Solvent Peracid 1 Log Reduction No. Solvent (%) (ppm) Appearance B. Cereus C.
- Example 2 Using the method of Example 2, a sparingly soluble solvent was added to various additional antimicrobial agents and tested against Bacillus cereus, Bacillus subtilis, C. funicola and N. fisheri using a 10 second contact time at 60° C.
- Table VIII Set out below in Table VIII are the run number, solvent and antimicrobial agent employed, solvent amount, antimicrobial agent amount, and the observed log order reduction for Bacillus cereus, Bacillus cereus, C. funicola, or N. fisheri for each composition. TABLE VIII Run Solvent + Additional Solvent Additional Log Reduction No. Antimicrobial Agent(s) Amount Agent Amount B. cereus B. subtilis C. funicola N.
- Example 2 Using the method of Example 2, a composition was tested against the spore Bacillus cereus and the mold C. funicola using a 120 second contact time at 40° C. These experiments were run in order to determine the antimicrobial effectiveness of a composition of the present invention at a lower treatment temperature.
- Table IX Set out below in Table IX are the run number, solvent and additional antimicrobial agent, solvent amount, additional antimicrobial agent amount, and the observed log order reduction for Bacillus cereus and C. funicola for each composition. TABLE IX Solvent + Additional Additional Log Reduction Run No. Antimicrobial Agent Solvent (%) Agent Amount B. cereus C.
- Aqueous mixtures containing an antimicrobially-active solvent, a peracid, or mixtures of both were prepared and evaluated against the spore-forming, enterotoxin producing pathogen Bacillus cereus using a 10 second contact time at 60° C.
- Table X are the run number, solvent, solvent concentration, peracid concentration, and the observed log order reduction for B. cereus for each composition. TABLE X Run Solvent, Peracid B. cereus No.
- Run No. 10-7 provided nearly a 3-log reduction improvement compared to the use of the antimicrobially-active solvent or peracid alone, while using a lower quantity of peracid.
- Run No. 10-8 provided an especially effective sporicide at even lower levels of antimicrobially-active solvent and peracid.
- aqueous mixtures containing 3% benzyl alcohol, or varying amounts of several peracids KX-6091, MATRIXXTM, TSUNAMI 100TM or OXONIA ACTIVETM; Ecolab), or mixtures of both benzyl alcohol and peracid were prepared and evaluated as possible sterilant formulations against the spore-forming, enterotoxin producing pathogen Bacillus cereus using a 10 second contact time at 60° C.
- Table VIII are the run number, solvent, peracid concentration, and the observed log order reduction for B. cereus for each composition. TABLE XI Run Solvent, Peracid, B. cereus No.
- Run No. 11-2 provided more than a 6-log reduction improvement compared to the use of the antimicrobially-active solvent alone (Run No. 11-5), and nearly a 6-log reduction improvement compared to the use of the peracid alone (Run No. 11-7), yet required only one-fourth as much peracid.
- aqueous mixtures containing various solvents and varying amounts of a peracid (15C; Ecolab) were prepared and evaluated against the spore-forming, enterotoxin producing pathogen Bacillus cereus using a 10 second contact time at 60° C.
- Table XII are the run number, solvent type and concentration, peracid type and concentration, and the observed log order reduction for B. cereus for each composition. As shown, a wide range of chemical solvent classes yielded substantial spore reductions.
- aqueous mixtures containing varying types and amounts of solvents and varying types and amounts of several peracids were prepared and evaluated as sporicides against Bacillus subtilis , using a 10 second contact time at 60° C.
- Table XIII are the run number, solvent, solvent concentration, peracid type and concentration, and the observed log order reduction for B. subtilis for each composition. TABLE XIII B. subtilis Run Solvent Peracid Type and Log No.
- aqueous mixtures containing 2.5 wt. % DBE-3TMsolvent (diester blend, DuPont Nylon) and a peracid were prepared and evaluated as a general antimicrobial agent against S. aureus, E. coli , or N. fisheri using a 10 second contact time at 60° C.
- Table XIV are the run number, peracid type and amount, and the observed log order reduction for each organism. TABLE XIII Run Peracid Type and Log Reduction No. Amount (ppm) S. aureus E. coli N.
Abstract
A method for antimicrobial treatment comprising applying to microbes a composition containing a diluting solvent (e.g., water), an antimicrobially-active solvent having a density different from the density of the diluting solvent, and an optional cosolvent, surfactant, or additional antimicrobial agent, wherein the amount of antimicrobially-active solvent or additional antimicrobial agent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus within 10 seconds at 60° C. Preferred methods of the invention employ compositions containing an additional antimicrobial agent such as peroxyacetic acid. Compositions for use in the method can be prepared as concentrates, and used full strength or in diluted form.
Description
- This application is a continuation of application Ser. No. 10/413,635 filed Apr. 14, 2003, which is a divisional of application Ser. No. 09/794,790, filed Feb. 27, 2001, which is a continuation-in-part of application Ser. No. 09/560,170, filed Apr. 28, 2000, and a continuation-in-part of application Ser. No. 09/641,775, filed Aug. 18, 2000.
- This invention relates to compositions that can be used, for example, to clean, reduce the microbial population of, or sterilize surfaces, and to compositions that can be used for aseptic packaging.
- There has been a longstanding need for antimicrobial agents having improved antimicrobial efficacy and improved speed of action. The specific requirements for such agents vary according to the intended application (e.g., sanitizer, disinfectant, sterilant, aseptic packaging treatment, etc.) and the applicable public health requirements. For example, as set out in Germicidal and Detergent Sanitizing Action of Disinfectants, Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 960.09 and applicable sections, 15th Edition, 1990 (EPA Guideline 91-2), a sanitizer should provide a 99.999% reduction (5-log order reduction) within 30 seconds at room temperature, 25±2° C., against several test organisms.
- Many antimicrobial agents (e.g., iodophors, peracids, hypochlorites, chlorine dioxide, ozone, etc.) have a broad spectrum of antimicrobial properties. However, these agents sometimes have inadequate activity against bacterial spores, fungal spores, and fungi. Killing, inactivating, or otherwise reducing the active population of bacterial spores and fungi on surfaces is particularly difficult. Bacterial spores have a unique chemical composition of spore layers that make them more resistant than vegetative bacteria to the antimicrobial effects of chemical and physical agents. Likewise, the unique chemical composition of fungal cells, especially mold spores, makes them more resistant to chemical and physical agents than are other microorganisms. This resistance can be particularly troublesome when the spores or fungi are located on surfaces such as food, food contact sites, ware, hospitals and veterinary facilities, surgical implements, and hospital and surgical linens and garments.
- Control of the mold Chaetomium funicola, and of bacterial spore-forming microorganisms of the Bacillus species, can be especially important during food packaging, particularly during cold or hot aseptic filling of food and beverage products. Microorganisms of the Bacillus species include Bacillus cereus, Bacillus mycoides, Bacillus subtilis, Bacillus anthracis, and Bacillus thuringiensis. These latter microorganisms share many phenotypical properties, have a high level of chromosomal sequence similarity, and are known enterotoxin producers. Bacillus cereus is one of the most problematic because Bacillus cereus has been identified as possessing increased resistance to germicidal chemicals used to decontaminate environmental surfaces. For example, Blakistone et al., Efficacy of Oxonia Active Against Selected Sporeformers, Journal of Food Protection, Volume 62, pp. 262-267, reported that Bacillus cereus was more tolerant to the effects of conventionally formulated peroxyacetic acid germicides than all other spore-forming bacteria tested, including other Bacillus and Clostridium species.
- Bacillus cereus is frequently diagnosed as a cause of gastrointestinal disorders and has been suggested to be the cause of several food-borne illness outbreaks. Due to its rapid sporulating capacity, Bacillus cereus easily survives in the environment. Bacillus cereus is omnipresent in nature, and consequently can usually be found in animal feed and fodder. Bacillus cereus can contaminate raw milk via feces and soil, and can survive intestinal passage in cows and the pasteurization process.
- Bacillus cereus is also known to cause serious human illness via environmental contamination. For example, Bacillus cereus is known to cause post-traumatic injury eye infections, which can result in visual impairment or loss of vision within 12-48 hours after infection. In addition, Bacillus cereus is regarded as transferable from washed surgical garments to patients.
- Agents having greater or faster activity against bacterial spores, fungi, and other resistant microorganisms (particularly microorganisms of the Bacillus species) could help meet a substantial public health need, and one that is not adequately addressed by current commonly-used antimicrobial agents.
- The present invention provides, in one aspect, a method for antimicrobial treatment comprising applying to microbes a composition containing a diluting solvent (e.g., water), an antimicrobially-active solvent having a density different from the density of the diluting solvent, and an optional cosolvent, surfactant, or additional antimicrobial agent, wherein the amount of antimicrobially-active solvent or additional antimicrobial agent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus within 10 seconds at 60° C. In a preferred aspect, the methods of the invention provide broader spectrum antimicrobial action, providing greater than a 1-log order reduction within 10 seconds at 60° C. in one or more additional organisms such as the mold Chaetomium funicola. In a more preferred aspect, the methods of the invention provide greater than a 1-log order reduction within 10 seconds at 60° C. in Chaetomium funicola, Bacillus subtilis and Bacillus cereus.
- In another aspect, the invention provides a method for antimicrobial treatment, comprising applying to microbes a composition as described above, wherein the composition further comprises such additional antimicrobial agent. In a particularly preferred embodiment, the additional antimicrobial agent comprises a peracid such as peroxyacetic acid; a peroxide such as hydrogen peroxide; or a halogen containing compound such as hypochlorous acid (or its salts), chlorine dioxide, hypobromous acid (or its salts), or an interhalide such as iodine monochloride, iodine dichloride, iodine tetrachloride, bromine chloride, iodine monobromide, or iodine dibromide.
- In yet another aspect, the invention provides an antimicrobial concentrate and instructions for mixing the concentrate with water, wherein the concentrate comprises an antimicrobially-active solvent that has a density different from that of water, an optional cosolvent or surfactant, and an optional additional antimicrobial agent, the amounts of antimicrobially-active solvent and optional additional antimicrobial agent being sufficiently high and the amount of cosolvent or surfactant being sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus within 10 seconds at 60° C. In a particularly preferred embodiment, the composition comprises said additional antimicrobial agent and the amount of antimicrobially-active solvent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition does not form a clear single-phase solution or microemulsion when the concentrate is mixed with water according to the instructions.
- In a further aspect, the invention provides an antimicrobial composition comprising a diluting solvent, an antimicrobially-active solvent having a density that is different from the density of the diluting solvent, an additional antimicrobial agent, and an optional cosolvent or surfactant, the amounts of antimicrobially-active solvent and of additional antimicrobial agent being sufficiently high and the amount of cosolvent or surfactant being sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus or the mold Chaetomium funicola within 10 seconds at 60° C.
- In yet another aspect, the invention provides an antimicrobial concentrate and instructions for mixing the concentrate with water, wherein the concentrate comprises an antimicrobially-active solvent that has a density different from that of water, an optional cosolvent or surfactant, and an additional antimicrobial agent, the amounts of antimicrobially-active solvent and additional antimicrobial agent being sufficiently high so that the composition will provide greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus or the mold Chaetomium funicola within 10 seconds at 60° C. In a particularly preferred embodiment, the composition comprises a sufficiently high amount of additional antimicrobial agent and antimicrobially-active solvent such that the composition forms a clear single-phase solution when the concentrate is mixed with water according to the instructions, and provides greater than a 1-log order reduction in the population of bacteria or spores of Bacillus cereus or Bacillus subtilis and in the population of the mold Chaetomium funicola within 10 seconds at 60° C.
- The method and compositions of the invention are especially useful for aseptic packaging, re-use clean-in-place (CIP) or clean-out-of-place (COP) systems, hospital disinfectants, veterinary clinic disinfectants, and as sporicides or sterilants.
- As used in this invention, the term “sterilant” refers to a physical or chemical agent or process capable of destroying all forms of life (including bacteria, viruses, fungi, and spores) on inanimate surfaces. One procedure is described in A.O.A.C. Sporicidal Activity of Disinfectants, Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 966.04 and applicable sections, 15th Edition, 1990 (EPA Guideline 91-2)
- As used in this invention, the term “antimicrobial composition” refers to a composition having the ability to cause greater than a 90% reduction (1-log order reduction) in the population of bacteria or spores of Bacillus species within 10 seconds at 60° C., using the above-mentioned Germicidal and Detergent Sanitizing Action of Disinfectants procedure. Preferably, Bacillus cereus or Bacillus subtilis are used in such procedure. Also preferably, the antimicrobial compositions of the invention provide greater than a 99% reduction (2-log order reduction), more preferably greater than a 99.99% reduction (4-log order reduction), and most preferably greater than a 99.999% reduction (5-log order reduction) in such population within 10 seconds at 60° C. Preferably, the antimicrobial compositions of the invention also provide greater than a 99% reduction (2-log order reduction), more preferably greater than a 99.99% reduction (4-log order reduction), and most preferably greater than a 99.999% reduction (5-log order reduction) in the population of one or more additional organisms such as the mold Chaetomium funicola. Because in their broadest sense these definitions for antimicrobial activity are different from some of the current governmental regulations, the use in connection with this invention of the term “antimicrobial” is not intended to indicate compliance with any particular governmental standard for antimicrobial activity.
- As used in this invention, the term “sporicide” refers to a physical or chemical agent or process having the ability to cause greater than a 90% reduction (1-log order reduction) in the population of spores of Bacillus cereus or Bacillus subtilis within 10 seconds at 60° C. Preferably, the sporicidal compositions of the invention provide greater than a 99% reduction (2-log order reduction), more preferably greater than a 99.99% reduction (4-log order reduction), and most preferably greater than a 99.999% reduction (5-log order reduction) in such population within 10 seconds at 60° C.
- As used in this invention, the term “sanitizer” refers to an agent that reduces the number of bacterial contaminants to safe levels as judged by public health requirements. Preferably, sanitizers for use in this invention will provide at least a 99.999% reduction (5-log order reduction) using the Germicidal and Detergent Sanitizing Action of Disinfectants procedure referred to above.
- As used in this invention, the term “disinfectant” refers to an agent that kills all vegetative cells including most recognized pathogenic microorganisms, using the procedure described in A.O.A.C. Use Dilution Methods, Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 955.14 and applicable sections, 15th Edition, 1990 (EPA Guideline 91-2).
- As used in this invention, the term “preservative” refers to an agent that extends the storage life of food and non-food products by retarding or preventing deterioration of flavor, odor, color, texture, appearance, nutritive value, or safety. A preservative need not provide a lethal, irreversible action resulting in partial or complete microbial cell destruction or incapacitation. Sterilants, sanitizers, disinfectants, sporicides, viracides and tuberculocidal agents provide such an irreversible mode of action, sometimes referred to as “bactericidal” action. In contrast, a preservative can provide an inhibitory or bacteriostatic action that is reversible, in that the target microbes can resume multiplication if the preservative is removed. The principal differences between a preservative and a sanitizer primarily involve mode of action (a preservative prevents growth rather than killing microorganisms) and exposure time (a preservative has days to months to act whereas a sanitizer has at most a few minutes to act).
- When applied to microbes (e.g., when applied to a surface containing microbes), the compositions of the invention exhibit antimicrobial action. The mechanism by which such action takes place is not completely understood. However, as shown in the Examples set out below, very rapid and substantially complete antimicrobial action can be attained.
- Some preferred compositions and methods of the invention provide “pseudo-stable” antimicrobial compositions that phase-separate following application of the composition to a surface. These compositions can also be described as exhibiting “phase-splitting” characteristics. The term “phase” refers to a homogeneous fluid portion that is present in or that can form in a fluid system. The term “phases” refers to the presence of more than one phase in a heterogeneous fluid system. The term “pseudo-stable” refers to a composition that forms a single phase when subjected to mild mixing or other agitation and retains that single phase for a sufficient period of time so that the composition can be applied to a surface, but which will promptly form two or more phases when left undisturbed. The term “phase-splitting” is meant to describe a single phase antimicrobially-active solvent-containing composition that forms at least two laminar phases promptly after being applied atop a generally horizontal surface or on a generally vertical surface, whereby a film containing a concentrated amount of the antimicrobially-active solvent lies between the surface and a film containing a much lower amount of the antimicrobially-active solvent. In a composition that has undergone phase splitting, the phase containing a concentrated amount of the antimicrobially-active solvent will be referred to as the solvent phase, and the phase containing a much lower amount of the antimicrobially-active solvent will be referred to as the dilute phase or diluting phase. For example, on counters, floors and other generally horizontal surfaces, the solvent phase will lie atop the surface (or atop microbes on the surface) and under the dilute phase or phases. On walls or other generally vertical surfaces, the solvent phase will lie adjacent the surface (or adjacent microbes on the surface) and under the dilute phase or phases. In such compositions, as is described in more detail below, attainment of pseudo-stable phase-splitting behavior can be achieved by employing a sufficiently high amount of antimicrobially-active solvent and a sufficiently low amount of cosolvent or surfactant.
- In some compositions of the invention (and in some methods of the invention employing such compositions), the amount of antimicrobially-active solvent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition forms a “quasi-stable” antimicrobial composition. Such compositions have a clear or slightly cloudy appearance, do not form a clear single-phase solution or microemulsion, and do not undergo phase-splitting. However, they are antimicrobial compositions as defined herein. If in such quasi-stable compositions the amount of antimicrobially-active solvent is increased sufficiently, or if the amount of cosolvent or surfactant is decreased sufficiently, then these compositions will become pseudo-stable. Thus, these quasi-stable compositions almost exhibit pseudo-stable behavior, and will do so if modified as taught herein. As shown in some of the Examples set out below, these quasi-stable compositions can provide significant antimicrobial activity even though they do not undergo phase-splitting during use.
- For simplicity, the remainder of this specification will discuss compositions that upon standing will form clear one-phase mixtures, cloudy two-phase dispersions or phase-splitting two-phase mixtures, it being understood that compositions forming three or more phases upon standing could be employed if desired.
- The compositions of the invention can be formulated and sold for use as is, or as solvent concentrates. If desired, such concentrates can be used full-strength as antimicrobial agents. However, the concentrates typically will be diluted with a fluid (e.g., water) that subsequently forms the dilute phase. Preferably, the concentrate forms a single phase before such dilution and remains so while stored in the container in which it will be sold. When combined with water or other desired diluting fluid at an appropriate dilution level and subjected to mild agitation (e.g., by stirring or pumping the composition), some compositions of the invention will form a pseudo-stable dispersion, and other compositions of the invention will form a clear or quasi-stable solution or dispersion. If a pseudo-stable composition is formed, then the composition preferably remains in the pseudo-stable state for a sufficiently long period so that the composition can be applied to a surface before the onset of phase separation. The pseudo-stable state need only last for a few seconds when suitably rapid application techniques such as spraying are employed, or when agitation during application is employed. The pseudo-stable state desirably lasts for at least one minute or more after mixing and while the composition is stored in a suitable vessel, and preferably lasts for five minutes or more after mixing. Often normal refilling or replenishment of the applicator (e.g., by dipping the applicator in the composition) will provide sufficient agitation to preserve the pseudo-stable state of the composition during application.
- Some of the highest observed levels of antimicrobial activity have been observed using pseudo-stable antimicrobial compositions of he invention. However, very high levels have also been observed for some clear or quasi-stable antimicrobial compositions of the invention. For some applications these clear or quasi-stable antimicrobial compositions solutions or dispersions will be preferred, as they require little or no mixing before or during use, and have a reduced tendency to separate during storage.
- A variety of fluids can be used as the diluting solvent, including water in its liquid form; steam; condensed gases and other supercritical fluids (e.g., CO2); perchloroethylene; oils such as silicone oils (e.g., siloxanes), gear oils, transaxle oils, mineral oils or vegetable oils; and carboxylic esters such as methyl soyate. Mixtures of diluting solvents can be used if desired. Especially useful oils include food grade or food-derived oils, flavorings, or fragrance oils. Preferably, the diluting solvent consists essentially of or consists of water in its liquid form. The remainder of this specification will primarily discuss the use of water in its liquid form as the diluting solvent, it being understood that other suitable fluids could be added to or substituted for water in its liquid form if desired.
- The compositions of the invention can contain a variety of antimicrobially-active solvents. The antimicrobially-active solvent preferably is insoluble, or only sparingly soluble, in the diluting solvent. Thus for compositions containing water as the diluting solvent, and for concentrates intended to be diluted with water, the antimicrobially-active solvent preferably will have a water solubility less than about 5 wt. %, more preferably less than about 3 wt. %, and most preferably less than about 2 wt. %.
- In general, the antimicrobially-active solvent is selected based upon the characteristics of the surface and microbes to which the antimicrobial composition will be applied and upon the nature of any coating, soil or other material that will be contacted by the antimicrobial composition and optionally removed from the surface. Polar solvents, and solvents that are capable of hydrogen bonding typically will perform well on a variety of surfaces and microbes and thus are preferred. Preferably, the antimicrobially-active solvent also has a high flashpoint (e.g., greater than about 30° C., more preferably greater than about 50° C., and most preferably greater than about 100° C.), low odor and low human and animal toxicity. Most preferably the antimicrobially-active solvent is a food-grade or cosmetic or flavorant additive.
- Preferred antimicrobially-active solvents having a density different from that of water (and thus especially useful in compositions that will be diluted with water and applied atop horizontal or generally horizontal surfaces) include acetamidophenol (specific gravity 1.027); acetanilide (specific gravity 1.219; water solubility<1%); acetophenone (specific gravity 1.0238; water solubility<1%); [2-acetyl-1-methylpyrrole (specific gravity 1.04); benzyl acetate (specific gravity 1.0515; water solubility<1%); benzyl alcohol (specific gravity 1.0413; water solubility ˜4%); benzyl benzoate (specific gravity 1.118; water solubility<1%); benzyloxyethanol (specific gravity 1.07; water solubility<1%); ethers or hydroxyethers such as ethylene glycol phenyl ether (specific gravity 1.104; water solubility 2.3%; commercially available as DOWANOL EPH™ from Dow Chemical Co.) and propylene glycol phenyl ether (specific gravity 1.063; water solubility 1.1%; commercially available as DOWANOL PPH™ from Dow Chemical Co.); essential oils (e.g., benzaldehyde, pinenes (alphas, betas, etc.), terpineols, terpinenes, carvone, cinnamealdehyde, bomeol and its esters, citrals, ionenes, jasmine oil, limonene, dipentene, linalool and its esters); dibasic esters such as dimethyl adipate, dimethyl succinate, dimethyl glutarate (often available in a mix with specific gravities greater than 1.00; including products available under the trade designations DBE, DBE-3, DBE-4, DBE-5, DBE-6, DBE-9, DBE-IB, and DBE-ME from DuPont Nylon), dimethyl malonate, diethyl adipate, diethyl succinate, diethyl glutarate, dibutyl succinate, and dibutyl glutarate; dialkyl carbonates such as dimethyl carbonate, diethyl carbonate, dipropyl carbonate, diisopropyl carbonate, and dibutyl carbonate; C1-16 protonated carboxylic acids such as 2-ethyl-1-hexanoic acid, butyric acid, octanoic acid, heptanoic acid, nonanoic acid, and decanoic acid; C1-12 organic anhydrides such as acetic anhydride, succinic anhydride, phthalic anhydride, maleic anhydride, and alkyl or alkenyl succinic anhydrides; organo-nitriles such as acetonitrile and benzonitrile; organo-phosphates and phosphonates such as tributyl phosphate, tripropyl phosphate, 2-ethyl-1-hexyl phosphate; and phthalate esters such as dibutyl phthalate, diethylhexyl phthalate, and diethyl phthalate. The water solubilities noted above are room temperature values. Benzyl alcohol, phenylethanol, essential oils, dibasic esters, dialkyl carbonates, ethylene glycol phenyl ether and propylene glycol phenyl ether are particularly preferred antimicrobially-active solvents. Mixtures of antimicrobially-active solvents can be used if desired.
- The compositions of the invention should contain sufficient antimicrobially-active solvent to provide the desired rate and type of microbial reduction. Usually, antimicrobial concentrates of the invention will contain at least about 5 wt. % antimicrobially-active solvent, preferably at least about 25 wt. % antimicrobially-active solvent, more preferably at least about 65 wt. % antimicrobially-active solvent, and most preferably about 75 to about 95 wt. % antimicrobially-active solvent.
- The compositions of the invention can contain one or more cosolvents or surfactants to assist in providing pseudo-stable or quasi-stable behavior. In general, cosolvents or surfactants that are present at concentrations below those at which single-phase coupling arises, or cosolvents or surfactants that are relatively inefficient or ineffective (with respect to their ability completely to solubilize or disperse the antimicrobially-active solvent in the diluting solvent and form a single-phase system), are preferred over cosolvents or surfactants that are present at higher concentrations or are more efficient or effective. This differs from the approach normally taken when formulating compositions containing cosolvents or surfactants. Normally, cosolvents and surfactants are selected for their ability to promote formation of stable single-phase solutions, microemulsions, or dispersions.
- A variety of cosolvents can be employed. In general, the cosolvent is selected based upon the characteristics of the chosen antimicrobially-active solvent and the solubility of the chosen antimicrobially-active solvent in the diluting solvent. For compositions in which water serves as the diluting solvent, the cosolvent generally will have higher water solubility than the water solubility of the chosen solvent. Preferably, the cosolvent has a high flashpoint (e.g., greater than about 30° C., more preferably greater than about 50° C., and most preferably greater than about 100° C.), low odor and low human and animal toxicity.
- Preferred cosolvents include 2-(2-aminoethoxy)ethanol, monoethanolamine, diethanolamine, triethanolamine, amyl acetate, amyl alcohol, butanol, 3-butoxyethyl-2-propanol, butyl acetate, n-butyl propionate, cyclohexanone, diacetone alcohol, diethoxyethanol, diethylene glycol methyl ether, diethylene glycol n-butyl ether, diisobutyl carbinol, diisobutyl ketone, dimethyl heptanol, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene glycol propyl ether, dipropylene glycol tert-butyl ether, ethanol, ethyl acetate, 2-ethylhexanol, ethyl propionate, ethylene glycol butyl ether, ethylene glycol methyl ether acetate, hexanol, isobutanol, isobutyl acetate, isobutyl heptyl ketone, isophorone, isopropanol, isopropyl acetate, methanol, methyl amyl alcohol, methyl n-amyl ketone, 2-methyl-1-butanol, methyl ethyl ketone, methyl isobutyl ketone, 1-pentanol, n-pentyl propionate, 1-propanol, n-propyl acetate, n-propyl propionate, propylene glycol n-butyl ether, propylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol n-propyl ether, tripropylene glycol methyl ether and tripropylene glycol n-butyl ether. Ethylene glycol butyl ether and dipropylene glycol n-butyl ether are particularly preferred cosolvents. Mixtures of cosolvents can be used if desired.
- Commercially available cosolvents (all of which are available from Union Carbide Corp.) include Butoxyethyl PROPASOL™, Butyl CARBITOL™ acetate, Butyl CARBITOL™, Butyl CELLOSOLVE™ acetate, Butyl CELLOSOLVE™, Butyl DIPROPASOL™, Butyl PROPASOL™, CARBITOL™PM-600, CARBITOL™ Low Gravity, CELLOSOLVE™ acetate, CELLOSOLVE™, Ester EEP™, FILMER IBT™, Hexyl CARBITOL™, Hexyl CELLOSOLVE™, Methyl CARBITOL™, Methyl CELLOSOLVE™ acetate, Methyl CELLOSOLVE™, Methyl DIPROPASOL™, Methyl PROPASOL™ acetate, Methyl PROPASOL™, Propyl CARBITOL™, Propyl CELLOSOLVE™, Propyl DIPROPASOL™ and Propyl PROPASOL™.
- The compositions of the invention preferably should not contain excessive cosolvent, as the use of an excess of cosolvent will tend to cause formation of an antimicrobially inert single-phase solution or microemulsion. Instead, the amount of cosolvent preferably is just sufficient to provide the desired level of antimicrobial activity. Larger amounts of cosolvent may diminish the antimicrobial effectiveness of the compositions of the invention. Usually, antimicrobial concentrates of the invention will contain 0 to about 50 wt. % cosolvent, more preferably 0 to about 25 wt. % cosolvent, and most preferably 0 to about 20 wt. % cosolvent.
- A variety of surfactants can be employed. In general, the surfactant identity and use level is selected based upon the characteristics of the chosen antimicrobially-active solvent and the solubility of the chosen antimicrobially-active solvent in the diluting solvent. For compositions in which water serves as the diluting solvent, the surfactant preferably will have an HLB value greater than or equal to about 13, or less than or equal to about 6. This value reflects the above-noted preference in the present invention for employing surfactants that are relatively inefficient or ineffective as emulsifiers. Preferably, the surfactant does not tend to cause formation of insoluble deposits, and has low odor and low toxicity. Mixtures of surfactants can be used if desired.
- Preferred anionic surfactants include C6-C24 alkylbenzene sulfonates; C6-C24 olefin sulfonates; C6-C24 paraffin sulfonates; cumene sulfonate; xylene sulfonate; C6-C24 alkyl naphthalene sulfonates; C6-C24 alkyl or dialkyl diphenyl ether sulfonates or disulfonates, C4-C24 mono or dialkyl sulfosuccinates; sulfonated or sulfated fatty acids; C6-C24 alcohol sulfates (preferably C6-C12 alcohol sulfates); C6-C24 alcohol ether sulfates having 1 to about 20 ethylene oxide groups; and C4-C24 alkyl, aryl or alkaryl phosphate esters or their alkoxylated analogues having 1 to about 40 ethylene, propylene or butylene oxide units or mixtures thereof.
- Preferred nonionic surfactants include C6-C24 alcohol ethoxylates (preferably C6-C14 alcohol ethoxylates) having 1 to about 20 ethylene oxide groups (preferably about 9 to about 20 ethylene oxide groups); C6-C24 alkylphenol ethoxylates (preferably C8-C10 alkylphenol ethoxylates) having 1 to about 100 ethylene oxide groups (preferably about 12 to about 20 ethylene oxide groups); C6-C24 alkylpolyglycosides (preferably C6-C20 alkylpolyglycosides) having 1 to about 20 glycoside groups (preferably about 9 to about 20 glycoside groups); C6-C24 fatty acid ester ethoxylates, propoxylates or glycerides; and C4-C24 mono or di alkanolamides.
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- where R, R′, R″ and R′″ are each a C1-C24 alkyl, aryl or aralkyl group that can optionally contain one or more P, O, S or N heteroatoms, and X is F, Cl, Br, I or an alkyl sulfate.
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- where R, R′, R″ and R′″ are each a C1-C24 alkyl, aryl or aralkyl group that can optionally contain one or more P, O, S or N heteroatoms.
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- where R, R′, R″ and R′″ are each a C1-C24 alkyl, aryl or aralkyl group that can optionally contain one or more P, O, S or N heteroatoms, and n is about 1 to about 10.
- The antimicrobial compositions of the invention should not contain excessive amounts of surfactant, lest an antimicrobially inactive single-phase solution or microemulsion be formed. Instead, the amount of surfactant should be just sufficient to provide the desired level of antimicrobial activity. Larger amounts of surfactant may diminish the antimicrobial effectiveness of the compositions of the invention. Usually, the solvent concentrates of the invention will contain no more than about 10 wt. % surfactant, more preferably 0 to about 3 wt. % surfactant and most preferably 0 to about 1 wt. % surfactant. Most preferably, the concentrates are substantially surfactant-free.
- The antimicrobial compositions of the invention preferably contain an additional antimicrobial agent. This additional antimicrobial agent can be dissolved or dispersed in the antimicrobially-active solvent or in the diluting solvent. Desirably, the additional antimicrobial agent will preferentially dissolve or disperse in the antimicrobially-active solvent rather than in the diluting solvent. Suitable additional antimicrobial agents include carboxylic acids, diacids, or triacids (e.g., butyric acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, salycic acid, mandelic acid, succinic acid, adipic acid, glutaric acid, EDTA and citric acid), carboxylic esters (e.g., p-hydroxy alkyl benzoates and alkyl cinnamates), sulfonic acids (e.g., dodecylbenzene sulfonic acid), iodo-compounds or active halogen compounds (e.g., iodine, interhalides, polyhalides, metal hypochlorites, hypochlorous acid, metal hypbromites, hypobromous acid, chloro- and bromo-hydantoins, chlorine dioxide and sodium chlorite), active oxygen compounds including hydrogen peroxide, isolated or equilibrium derived or isolated peracids such as chloroperbenzoic acids, peracetic acid, perheptanoic acid, peroctanoic acid, perdecanoic acid, performic acid, percitric acid, perglycolic acid, perlactic acid, perbenzoic acid, and monoester peracids derived from diacids or diesters (e.g., such as adipic, succinic, glutaric, or malonic acid and mixtures thereof), organic peroxides including benzoyl peroxide, alkyl benzoyl peroxides, ozone, singlet oxygen generators, and mixtures thereof, phenolic derivatives (e.g., o-phenyl phenol, o-benzyl-p-chlorophenol, tert-amyl phenol and C1-C6 alkyl hydroxy benzoates), quaternary ammonium compounds (e.g., alkyldimethylbenzyl ammonium chloride, dialkyldimethyl ammonium chloride and mixtures thereof), and mixtures of such antimicrobial agents, in an amount sufficient to provide the desired degree of microbial protection. Most of the aforementioned additional antimicrobial agents having about 1-6 carbons, or an ionic charge, would be mostly soluble in the diluting solvent; those with higher carbon numbers would generally be more soluble in the antimicrobially-active solvent. In either case, for a pseudo-stable antimicrobial composition it is preferred to use additional antimicrobial agents that can be drawn into the solvent phase or onto surfaces during phase separation.
- Compositions of the invention containing such optional additional antimicrobial agents appear to have substantially greater antimicrobial effectiveness than comparison aqueous solutions or dispersions containing the additional antimicrobial agent alone. If present in the antimicrobial concentrates of the invention, the additional antimicrobial agent preferably is about 0.01 to about 30 wt. % of the concentrate, more preferably about 0.05 to about 10 wt. % and most preferably about 0.1 to about 5 wt. %.
- If desired, the antimicrobial compositions of the invention can contain various adjuvants such as chelants, builders, thickeners, fragrances, dyes, pH adjusters, anticorrosion additives, antirust additives and indicators. The types and amounts of such adjuvants will be apparent to those skilled in the art.
- The compositions of the invention can be formulated to include the diluting solvent (e.g., water) as sold, or the diluting solvent can be added at any time up to the time of use. Preferably, the concentrates of the invention contain little or no diluting solvent as sold. A variety of dilution ratios can be employed, so long as the diluted composition exhibits the desired antimicrobial behavior when applied to the target microbes. The ingredients in the concentrate can represent about 1 to about 99 wt. % of the diluted mixture, more preferably about 5 to about 50 wt. %, and most preferably about 6 to about 25 wt. %. The diluted antimicrobial compositions preferably contain about 0.01 to about 50 wt. % of the antimicrobially-active solvent, with concentrations of about 0.1 to 10 wt. % being more preferred and concentrations of about 0.5 to about 5 wt. % being most preferred. As a further guide, the diluted composition preferably contains antimicrobially-active solvent in an amount near the solubility limit of the antimicrobially-active solvent in the diluting solvent. In addition, the diluted antimicrobial compositions preferably are aqueous, contain additional antimicrobial agent, and are clear or quasi-stable.
- The compositions of the invention can be sold in the form of a kit containing the composition together with suitable directions for carrying out the method of the invention. Such directions typically will include recommended dilution ratios, applications, application techniques and safety warnings.
- Although no longer commercially available, an aqueous floor stripping agent concentrate previously sold in Canada as Fuller Formula 3100™ Super Concentrate (Fuller Brush, Québec) could be used as an antimicrobial composition of the invention. However, to do so the concentrate should be diluted at a ratio not recommended in the product instructions. Fuller Formula 3100™ Super Concentrate is believed to have contained about 49 wt. % benzyl alcohol, 17 wt. % monoethanolamine, 10 wt. % sodium decyldiphenyl ether disulfonate and 24 wt. % water. Dilution of the concentrate at a 1:20 concentrate:water ratio was recommended on the product instructions. At that dilution ratio, the resulting mixture formed a stable single-phase solution. However, if diluted at a sufficiently larger concentrate:water ratio, the resulting mixture forms a quasi-stable or pseudo-stable composition. For example, at a 1:10 concentrate:water ratio, the composition is pseudo-stable and will undergo phase splitting when applied to a substrate and allowed to stand for a few minutes.
- The antimicrobial compositions of the invention can be used for a variety of domestic or industrial applications, e.g., to reduce microbial or viral populations on a surface or object or in a body or stream of water. The compositions can be applied in a variety of areas including kitchens, bathrooms, factories, hospitals, dental offices and food plants, and can be applied to a variety of hard or soft surfaces having smooth, irregular or porous topography. Suitable hard surfaces include, for example, architectural surfaces (e.g., floors, walls, windows, sinks, tables, counters and signs); eating utensils; hard-surface medical or surgical instruments and devices; and hard-surface packaging. Such hard surfaces can be made from a variety of materials comprising, for example, ceramic, metal, glass, wood or hard plastic. Suitable soft surfaces include, for example paper; filter media, hospital and surgical linens and garments; soft-surface medical or surgical instruments and devices; and soft-surface packaging. Such soft surfaces can be made from a variety of materials comprising, for example, paper, fiber, woven or nonwoven fabric, soft plastics and elastomers. The compositions of the invention can also be applied to soft surfaces such as food and skin. The compositions are also suitable for application to growing or harvested plant material including leaves, stems, tubers, roots, seeds, and the like.
- The antimicrobial compositions of the invention can be included in products such as sterilants, sanitizers, disinfectants, preservatives, deodorizers, antiseptics, fungicides, germicides, sporicides, virucides, detergents, bleaches, hard surface cleaners, hand soaps and pre- or post-surgical scrubs. The compositions have particular utility as cold or hot aseptic packaging treatments. The antimicrobial compositions can also be used in veterinary products such as mammalian skin treatments or in products for sanitizing or disinfecting animal enclosures, pens, watering stations, and veterinary treatment areas such as inspection tables and operation rooms.
- The antimicrobial compositions of the invention can be used for treating skin diseases on animals (especially mammals), or those which spread via transfer to air or surface substrates, such as disease from fungi, molds, bacteria spores and viruses. These spreadable skin diseases include athletes foot fungus and hairy hoof wart disease, and the many organisms leading to Mastitis and other mammalian milking diseases. The disease can be a viral disease such as parvovirus, coxsackie virus, or herpes virus. The disease can also be bacterial, such as S. aureus, E. coli, Streptococci, etc., or a Mycobacterium type such as that leading to tuberculosis. The compositions may also be used to treat animal carcasses to reduce both pathogenic and non-pathogenic microbial levels.
- The antimicrobial compositions can also be used on foods and plant species to reduce surface microbial populations; used at manufacturing or processing sites handling such foods and plant species; or used to treat process waters around such sites. For example, the compositions can be used on food transport lines (e.g., as belt sprays); boot and hand-wash dip-pans; food storage facilities; anti-spoilage air circulation systems; refrigeration and cooler equipment; beverage chillers and warmers, blanchers, cutting boards, third sink areas, and meat chillers or scalding devices. The compositions of the invention can be used to treat produce transport waters such as those found in flumes, pipe transports, cutters, slicers, blanchers, retort systems, washers, and the like.
- The antimicrobial compositions have particular value for use on food packaging materials and equipment, and especially for cold or hot aseptic packaging. The compositions can also be used on or in ware wash machines, dishware, bottle washers, bottle chillers, warmers, third sink washers, cutting areas (e.g., water knives, slicers, cutters and saws) and egg washers. Particular foodstuffs that can be treated with compositions of the invention include eggs, meats, seeds, leaves, fruits and vegetables. Particular plant surfaces include both harvested and growing leaves, roots, seeds, skins or shells, stems, stalks, tubers, corms, fruit, and the like. Particular treatable surfaces include packaging such as cartons, bottles, films and resins; dish ware such as glasses, plates, utensils, pots and pans; ware wash machines; exposed food preparation area surfaces such as sinks, counters, tables, floors and walls; processing equipment such as tanks, vats, lines, pumps and hoses (e.g., dairy processing equipment for processing milk, cheese, ice cream and other dairy products); and transportation vehicles.
- The antimicrobial compositions can also be used on or in other industrial equipment and in other industrial process streams such as heaters, cooling towers, boilers, retort waters, rinse waters, aseptic packaging wash waters, and the like. The compositions can be used to treat microbes and odors in recreational waters such as in pools, spas, recreational flumes and water slides, fountains, and the like.
- The antimicrobial compositions can also be used to reduce microbial and viral counts in air and liquids by incorporation into filtering media or breathing filters, e.g., to remove water and air-born pathogens such as Legionella.
- Other hard surface cleaning applications for the antimicrobial compositions of the invention include clean-in-place systems (CIP), clean-out-of-place systems (COP), washer-decontaminators, sterilizers, textile laundry machines, ultra and nano-filtration systems and indoor air filters. COP systems can include readily accessible systems including wash tanks, soaking vessels, mop buckets, holding tanks, scrub sinks, vehicle parts washers, non-continuous batch washers and systems, and the like. CIP systems include a variety of devices that will be familiar to those skilled in the art, and will typically employ flow rates on the order of about 40 to about 600 liters per minute, temperatures from ambient up to about 70° C., and contact times of at least about 10 seconds, more preferably about 30 to about 120 seconds.
- The antimicrobial compositions can be applied to microbes or to soiled or cleaned surfaces using a variety of methods. For example, the antimicrobial composition can be sprayed or wiped onto a surface; the composition can be caused to flow over the surface, or the surface can be dipped into the composition. The compositions can be formulated as liquids, gels, aerosols, waxes, solids, or powders. If steam or another gaseous diluting solvent is employed, then the compositions can be formulated to be applied in a gaseous state.
- The invention is further illustrated in the following non-limiting examples, in which all parts and percentages are by weight unless otherwise indicated. In the examples the following procedures were employed:
- Several compositions were evaluated by comparing them against a commercially available aseptic bottle washing biocide based on mixed peracids (MATRIXX™; Ecolab). Compositions containing only 1000 ppm or 2000 ppm of a single peracid or mixed peracids were used as controls. The remaining compositions were prepared by adding 10% of various solvents to an aqueous solution containing 1000 ppm or 2000 ppm of the mixed peracids. Non-solubilizing amounts of anionic surfactants were added to some of the compositions to affect minimal coupling and to yield, in some cases, pseudo-stable behavior and at least a partial phase-splitting condition. Addition of such non-stabilizing amounts tended to provide partial coupling and improved antimicrobial solution stability but not necessarily improved microbial control.
- The compositions and controls were evaluated for antimicrobial activity using the procedure set out in set out in Germicidal and Detergent Sanitizing Action of Disinfectants, Official Methods of Analysis of the Association of Official Analytical Chemists, paragraph 960.09 and applicable sections, 15th Edition, 1990 (EPA Guideline 91-2), using a 10 second contact time at 60° C. against the mold Chaetomium funicola (C. funicola). This brief contact time presented an especially challenging test, as evidenced by low observed log order reduction values for the controls.
- Set out below in Table I are the run number, solvent, solvent description (in terms of its water solubility), peracid concentration, anionic surfactant concentration, appearance of the mixtures, after they had been allowed to stand for one minute, and observed log order reduction for C. funicola. The solvent description classified the solvents as highly soluble (>60% solubility in water), partially soluble (−20-60%), or sparingly soluble (<20%).
TABLE I Anionic Run Solvent Peracid Surfactant C. funicola No. Solvent Description Concentration Concentration Appearance Log Reduction 1-1 None None 2000 ppm1 0.0% clear 0.1 1-2 None None 1000 ppm2 0.26% clear 0.05 1-3 None None 2000 ppm2 0.52% clear 0.1 1-4 Glycolic acid highly soluble 2000 ppm2 0.62% clear 0.2 1-5 Dimethyl sulfoxide highly soluble 2000 ppm2 0.62% clear 0.3 1-6 Hydrocarbon diol3 partially soluble 2000 ppm2 0.62% slightly cloudy 0.4 1-7 Propylene carbonate partially soluble 2000 ppm2 0.62% slightly cloudy 1.6 1-8 Diester blend4 sparingly soluble 2000 ppm2 0.62% very cloudy 6.0 1-9 Diester blend4 sparingly soluble 2000 ppm1 0.0% very cloudy >4.4 1-10 Benzyl alcohol sparingly soluble 2000 ppm2 0.62% very cloudy 5.0 1-11 Benzyl alcohol sparingly soluble 2000 ppm1 0.0% very cloudy >4.7
1Peracetic acid from TSUNAMI ™ 100 (Ecolab)
2Peracid from MATRIXX ™, a commercial peracid (Ecolab)
3VARONIC ™ TD-1 (Goldschmidt Chemical)
4DBE ™ (Dupont Nylon)
The compositions containing partially soluble solvents (Run Nos. 1-6 and 1-7) exhibited some phase-splitting behavior. The compositions containing sparingly soluble solvents (Run Nos. 1-8 through 1-11) exhibited substantial phase-splitting behavior. The results in Table I demonstrate that the addition of partially soluble and sparingly soluble solvents provided a substantial improvement in the antimicrobial efficacy of a commercial aseptic wash product, as can be seen by comparing control Run Nos. 1-1 through 1-3 to Run Nos. 1-6 through 1-11. The improved performance of Run Nos. 1-8 through 1-11 was especially dramatic, in that the observed activity improvement was 5 or more orders of magnitude compared to control Run Nos. 1-1 through 1-3. Use of highly soluble solvents (Run Nos. 1-4 and 1-5) provided only a small improvement in antimicrobial efficacy. - Several antimicrobial compositions of the present invention were evaluated for biocidal control, using the method of Example 1, and compared to several commercial products and to formulations from several U.S. patents. The comparison compositions formed clear (single-phase) formulations when prepared according to instructions. The compositions of the invention formed pseudo-stable cloudy compositions that underwent phase splitting following application. All tested compositions were evaluated against the spore-forming, enterotoxin producing pathogens Bacillus cereus and Bacillus subtilis and the mold C. funicola using a 10 second contact time at 60° C. Set out below in Table II are the run number, benzyl alcohol amount, amounts of additional ingredients, appearance of the mixtures, after they had been allowed to stand for one minute, and observed log order reduction for B. cereus, Bacillus subtilis and C. funicola for each composition.
TABLE II Benzyl Run Alcohol B. cereus B. subtilis C. funicola No. Amount Additional Ingredient Amounts1 Appearance Log Reduction Log Reduction Log Reduction 2-1 5.0% DBS2 (0.1%) clear (1-phase) 0.2 0.0 >4.4 2-2 2.0% DBS2 (0.1%) clear (1-phase) 0.1 — >4.4 2-3 2.0% DBS2 (5.0%) clear (1-phase) 0.0 0.2 0.3 2-4 2.0% Nonionic surfactant3 (5.0%) clear (1-phase) 0.0 0.1 0.4 2-5 0.0% BUTYL CELLOSOLVE ™ (10.0%), clear (1-phase) 0.0 0.2 3.2 DBS2 (2.4%), anhydrous sodium metasilicate (2.0%)4 2-6 6.0% DBS2 (1.3%), ammonium hydroxide clear (1-phase) 0.0 0.1 >4.8 28% (0.2%), Na-octane-1-sulfonate 40% (1.0%),5 2-7 4.0% Ethanol (10%)6 clear (1-phase) 0.05 0.1 4.6 2-8 2.0% Ethanol (10%)7 clear (1-phase) 0.1 — 4.7 2-9 4.0% Glycerine (10%), DBS (2%) + other clear (1-phase) 0.1 0.0 4.7 chemicals8 2-10 4.0% Peracid9 (0.1%), NAS10 (0.24%) cloudy (2-phase) >6.3 — >4.4 2-11 3.5% Peracid11 (0.15%) cloudy (2-phase) >6.3 — >4.5 2-12 3.0% Peracid12 (0.1%), NAS10 (0.24%) cloudy (2-phase) >6.3 >6.7 3.8 2-13 3.0% Peracid9 (0.1%), NAS10 (0.24%) cloudy (2-phase) >6.5 >6.7 4.0 2-14 3.0% Peracid13 (0.1%) cloudy (2-phase) >6.2 — 3.4 2-15 0.0% Diester blend14 (5%), peracid11 (0.1%), cloudy (2-phase) 5.0 — >4.4 LAS (0.1%) 2-16 0.0% Diester blend14 (5%), H2O2 (2.1%)15 cloudy (2-phase) — 2.9 >4.4 2-17 0.0% Diester blend14 (5%), H2O2 (2.1%)15 cloudy (2-phase) — >6.0 >4.4 2-18 0.0% Diester blend14 (5%), NaOCl (0.02%)16 cloudy (2-phase) 6.0 >6.1 >4.8 2-19 5.0% NaOCl (0.02%)16 cloudy (2-phase) — — >4.8 2-20 0.0% Diester blend14 (2.5%), NaOCl (0.025%)17 clear (1-phase) >6.0 >6.1 >3.4
1The remainder of these compositions contained water
2DBS = dodecylbenzene sulfonate
3TERGITOL ™ 15-S-9 (Union Carbide)
4See Example 25 of U.S. Pat. No. 5,158,710
5See Example 10 of U.S. Pat. No. 5,849,682
6See Example 4 of U.S. Pat. No. 5,180,749
7See Example 1 of U.S. Pat. No. 5,180,749
8See Example 3 of U.S. Pat. No. 5,635,492, made with 0.1% “Rhamsan gum,” 1% phosphate buffer and 0.003% blue dye.
9VORTEXX ™ or MATRIXX ™ commercial peracids (Ecolab)
10NAS = sodium, 1-octane sulfonate
11KX-6091 commercial peracid (Ecolab)
1215C commercial peracid (Ecolab)
13TSUNAMI-100 ™ commercial peracid (Ecolab)
14DBE-3 ™ (Dupont Nylon)
15Aged >18 hours
16Acidified to pH = 5.0 with acetic acid
17Acidified to pH = 6.0 with acetic acid
Except as otherwise noted, the comparative compositions in Run Nos. 2-1 through 2-9 were prepared according to the listed examples of the cited patents or according to the mixing instructions of the cited commercial products. Each was found to yield a non-phase-splitting formulation. The compositions of the present invention in Run Nos. 2-10 through 2-19 yielded phase-splitting formulations that formed at least 2 phases. Run No. 2-20 yielded a pseudo-stable solution that was just slightly opaque but did not separate during the test time. The compositions of the invention exhibited significant antimicrobial efficacy against B. cereus, as well as broad-spectrum efficacy against B. subtilis and C. funicola. However, the composition of Run No. 2-19 underwent a chemical reaction and could not be employed at the desired active level against the Bacillus spores. - Using the method of Example 1, 5% portions of various sparingly soluble solvents were added to plain water or to commercial peracid bottle washing formulas (KX-6091, 15C, or VORTEXX™; Ecolab) and tested against the mold C. funicola using a 10 second contact time at 60° C. A non-emulsifying amount of the anionic surfactant sodium octene sulfonate was added to some of the compositions to slow down, but not prevent, phase-splitting. Set out below in Table III are the run number, solvent, peracid, peracid concentration, surfactant concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and observed log order reduction for C. funicola for each composition.
TABLE III Run Peracid Surfactant C. funicola No. Solvent Peracid Concentration Concentration Appearance Log Reduction 3-1 None KX-60911 2000 ppm 0 ppm clear (1-phase) 0.2 3-2 None 15C1 2000 ppm 2500 ppm clear (1-phase) 0.2 3-3 None VORTEXX1 2000 ppm 5960 ppm clear (1-phase) 0.1 3-4 Glycol solvents2 KX-60911 2000 ppm 0 ppm cloudy (2-phase) 1.3 3-5 Glycol solvents3 KX-60911 2000 ppm 0 ppm cloudy (2-phase) 3.7 3-6 Glycol solvents4 KX-60911 2000 ppm 0 ppm cloudy (2-phase) 3.2 3-7 Diester blend5 KX-60911 2000 ppm 1000 ppm7 cloudy (2-phase) >4.4 3-8 Glycol solvents6 None 0 ppm 1000 ppm7 cloudy (2-phase) 2.8 3-9 Glycol solvents6 KX-60911 1000 ppm 1000 ppm7 cloudy (2-phase) 4.3 3-10 Glycol solvents6 KX-60911 2000 ppm 1000 ppm7 cloudy (2-phase) >4.4 3-11 2-ethyl-1-hexanol KX-60911 2000 ppm 1000 ppm7 cloudy (2-phase) >4.2 3-12 Dipentene KX-60911 2000 ppm 1000 ppm7 cloudy (2-phase) 2.7 3-13 Amyl acetate KX-60911 2000 ppm 1000 ppm7 cloudy (2-phasc) 3.3 3-14 Benzyl alcohol None 0 ppm 0 ppm cloudy (2-phase) >4.2 3-15 Benzyl alcohol KX-60911 2000 ppm 0 ppm cloudy (2-phase) >4.2 3-16 Tetrabutyl ammonium 15C1 2000 ppm 2500 ppm cloudy (2-phase) 1.7 hydroxide7 3-17 Phenoxyethanol 15C1 2000 ppm 2500 ppm cloudy (2-phase) 3.9 3-18 Phenoxyethanol VORTEXX1 2000 ppm 5960 ppm cloudy (2-phase) 4.8
1Commercial peracid (Ecolab)
2DOWANOL DPM ™ (Dow Chemical Co.)
3DOWANOL PPH ™ (Dow Chemical Co.)
4DOWANOL DPNP ™ (Dow Chemical Co.)
5DBE-3 ™ (Dupont Nylon)
6DOWANOL EPH ™ (Dow Chemical Co.)
7Neutralized to pH = 3.7 with acetic acid.
- The compositions in Run Nos. 3-3 to 3-18 exhibited phase-splitting. The results in Table III demonstrate that substantial improvements in antimicrobial efficacy could be obtained by modifying all three commercial aseptic wash products, as can be seen by comparing control Run No. 3-1 with Run Nos. 3-4 through 3-7, 3-9 through 3-13 and 3-15; control Run No. 3-2 with Run Nos. 3-16 and 3-17; and control Run No. 3-3 with Run No. 3-18. Run Nos. 3-8 and 3-14 exhibited significant antimicrobial efficacy without an additional antimicrobial agent. A composition containing both a sparingly soluble antimicrobially-active solvent and an additional antimicrobial agent exhibited a synergistic improvement in performance compared to the use of either the antimicrobially-active solvent or the additional antimicrobial agent alone, as can be seen by comparing Run No. 3-10 with Run Nos. 3-1 and 3-8.
- Using the method of Example 2, varying amounts of several sparingly soluble solvents were added to commercial peracid bottle washing formulations (TSUNAMI-100™, MATRIXX™, or KX-6091; Ecolab) and tested against the mold C. funicola using a 10 second contact time at 60° C. The surfactant dodecylbenzene sulfonate (“DBS”) was added to some of the compositions to slow down, but not inhibit, phase-splitting. Set out below in Table IV are the run number, solvent, solvent concentration, peracid concentration, DBS concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and observed log order reduction for C. funicola for each composition.
TABLE IV Run Solvent Peracid DBS C. funicola No. Solvent (%) (ppm) (ppm) Appearance Log Reduction 4-1 Benzyl alcohol 10% 20001 1000 cloudy (2-phase) >4.4 4-2 Benzyl alcohol 10% 20001 0 cloudy (2-phase) 3.9 4-3 Benzyl alcohol 5% 20001 500 cloudy (2-phase) 4.1 4-4 Benzyl alcohol 1% 20001 100 clear (1-phase) 0.1 4-5 None 0% 20001 0 clear (1-phase) 0.1 4-6 Diester blend,4 10% 20002 1000 cloudy (2-phase) >4.4 4-7 Diester blend,4 10% 20002 0 cloudy (2-phase) >4.4 4-8 Diester blend,4 5% 20002 500 cloudy (2-phase) 4.2 4-9 Diester blend,4 2.5% 15002 0 hazy (2-phase) >4.4 4-10 Diester blend,4 2.5% 12002 0 hazy (2-phase) >4.4 4-11 Diester blend,4 1% 20002 100 hazy (2-phase) 1.2 4-12 None 0% 20002 0 clear (1-phase) 0.2 4-13 Diester blend,2 5% 10003 1000 cloudy (2-phase) >4.4 4-14 Diester blend,2 4% 10003 0 cloudy (2-phase) >4.4 4-15 Diester blend,2 3% 10003 0 cloudy (2-phase) 3.2 4-16 Diester blend,2 2% 10003 0 hazy (2-phase) 3.1 4-17 Diester blend,2 2.5% 15007 0 hazy (2-phase) >4.4 4-18 None 0% 10003 0 clear (1-phase) 0.05 4-19 Solvent Mixture5 5% 20001 0 cloudy (2-phase) 3.6 4-20 Solvent Mixture5 1% 20001 0 clear (1-phase) 0.6 4-21 Phenoxyethanol 5.0% 20003 0 cloudy (2-phase) 4.8 4-22 Phenoxyethanol 5.0% 20006 0 cloudy (2-phase) 3.9 4-23 Phenoxyethanol 2.5% 12006 0 hazy (2-phase) 3.0 4-24 Phenoxyethanol 2.5% 15006 0 hazy (2-phase) >4.0 4-25 Phenylethanol 3.0% 20006 0 cloudy (2-phase) >4.5 4-26 Tetrabutyl ammonium 5.0% 20006 0 cloudy (2-phase) >4.5 hydroxide, pH = 3.7
1TSUNAMI-100 ™ commercial peracid (Ecolab)
2DBE-3 ™ (Dupont Nylon)
3MATRIXX ™, mixed peracids (Ecolab)
4KX-6091 commercial peracid (Ecolab)
5Mixture containing 50% benzyl alcohol, 15% DOWANOL PPH ™ glycol solvent (Dow Chemical Co.), 15% BUTYL CARBITOL ™, 15% DOWANOL DPNB ™ glycol solvent (Dow Chemical Co.) and 5% SURFONIC 24-9 ™ nonionic surfactant (Huntsman Chemicals)
615C commercial peracid (Ecolab)
- For each of the antimicrobial compositions in Table IV, significant antimicrobial efficacy was obtained near, or just above, the solubility limit of the antimicrobially-active solvent in the diluting solvent. The results in Table IV show that substantial improvements in antimicrobial efficacy were obtained by modifying the commercial aseptic wash products, as can be seen by comparing control Run No. 4-5 with Run Nos. 4-1 through 4-3 and 4-19; control Run No. 4-12 with Run Nos. 4-6 through 4-11 and 4-22; and control Run No. 4-18 with Run Nos. 4-13 through 4-17. Compositions with and without added surfactant (DBS) exhibited increased antimicrobial activity, as can be seen, for example, from Run Nos. 4-1 through 4-3 and 4-6 through 4-11. Compositions containing mixtures of antimicrobially-active solvents are shown in Run Nos. 4-19 and 4-20.
- Using the method of Example 2, varying amounts of benzyl alcohol were added to commercial peracid bottle washing formulations (KX-6091, 15C, TSUNAMI-100™, and VORTEXX™; Ecolab) and tested against the spore-forming, enterotoxin producing pathogen Bacillus cereus and the mold C. funicola using a 10 second contact time at 60° C. Set out below in Table V are the run number, solvent, solvent concentration, peracid concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and the observed log order reduction for Bacillus cereus and C. funicola for each composition.
TABLE V Run Solvent Peracid1 Log Reduction No. Solvent (%) (ppm) Appearance B. Cereus C. funicola 5-1 None 0% 10001 clear 1.2 0.1 5-2 None 0% 20001 clear 6.2 0.2 5-3 None 0% 20002 clear — 0.1 5-4 None 0% 20003 clear 0.2 0.2 5-5 None 0% 20004 clear 0.1 0.1 5-6 Benzyl alcohol 10% 0 very cloudy5 — >4.4 5-7 Benzyl alcohol 10% 10001 very cloudy5 — >4.4 5-8 Benzyl alcohol 4.5% 0 very cloudy5 0.1 >4.4 5-9 Benzyl alcohol 4.5% 10001 very cloudy5 6.3 >4.4 5-10 Benzyl alcohol 4.0% 0 very cloudy6 — >4.4 5-11 Benzyl alcohol 4.0% 10001 very cloudy6 3.6 >4.4 5-12 Benzyl alcohol 4.0% 10003 very cloudy6 4.8 >4.4 5-13 Benzyl alcohol 3.5% 0 opaque6 — 4.2 5-14 Benzyl alcohol 3.5% 10001 opaque6 1.8 >4.4 5-15 Benzyl alcohol 3.5% 10002 + clear >6.3 >4.4> 15002 5-16 Benzyl alcohol 3.5% 20002 clear >6.3 4.5 5-17 Benzyl alcohol 3.5% 15003 clear >6.3 >4.5 5-18 Benzyl alcohol 3.5% 0 cloudy5 3.4 2.6 5-19 Benzyl alcohol 3.0% 10001 clear — 2.7 5-20 Benzyl alcohol 3.0% 10002 clear >6.5 3.8 5-21 Benzyl alcohol 3.0% 20002 clear >6.3 >4.7 5-22 Benzyl alcohol 3.0% 25002 clear >6.3 >4.5 5-23 Benzyl alcohol 3.0% 15002 clear >6.3 >4.5 5-24 Benzyl alcohol 3.0% 10003 clear >6.5 4.3 5-25 Benzyl alcohol 3.0% 10004 cloudy5 >6.5 4.0 5-26 Benzyl alcohol 3.0% 10001 hazy >6.2 4.0 5-27 Benzyl alcohol 2.5% 10003 clear 6.4 4.2 5-28 Benzyl alcohol 2.5% 10001 cloudy5 3.0 4.0 5-29 Benzyl alcohol 2.0% 10003 clear 4.3 2.3 5-30 Benzyl alcohol 2.0% 10002 cloudy5 4.7 4.0 5-31 Benzyl alcohol 2.0% 10001 clear 3.7 3.8 5-32 Benzyl alcohol 1.5% 10003 clear 5.9 1.2 5-33 Benzyl alcohol 1.5% 0 cloudy5 0.05 0.2
1KX-6091 commercial peracid (Ecolab)
215C commercial peracid (Ecolab)
3VORTEXX ™ commercial peracid (Ecolab)
4TSUNAMI-100 ™ commercial peracid (Ecolab)
5Rapid phase separation.
6Slow phase separation.
The results in Table V show substantial enhancement in antimicrobial efficacy for compositions both above and below the water solubility limit (as evidenced visually by solution clarity) of the antimicrobially-active solvent in the diluting solvent. Significant antimicrobial efficacy was also obtained against both organisms using some clear solutions (see, e.g., Run Nos. 5-15 through 5-17, 5-20 through 5-24, 5-27, 5-29, 5-31 and 5-32). - 15 Aqueous mixtures containing 3% or 1% benzyl alcohol solvent and 2000 ppm or 1000 ppm of a commercial peracid bottle washing formulation (KX-6091 or VORTEXX™; Ecolab) were prepared. A surfactant was added to some of the mixtures. The mixtures were tested against the mold C. funicola using a 10 second contact time at 60° C. Set out below in Table VI are the run number, solvent concentration, peracid, surfactant, appearance of the mixtures after they had been allowed to stand for 1 minute, and the observed log order reduction for C. funicola for each composition.
TABLE VI C. funicola Run No. Solvent, % Peracid (ppm) Surfactant Appearance Log Reduction 6-1 (3%) 2000 ppm1 None cloudy, phase 3.4 separating 6-2 (3%) 2000 ppm1 mixed2 clear, 1-phase 0.2 microemulsion 6-3 (1%) 1000 ppm3 None clear, 1-phase 0.2 6-4 (1%) 1000 ppm3 LAS-MIPA4 cloudy, phase 2.8 separating
1KX-6091 commercial peracid (Ecolab)
21000 ppm of a mixture of 20% mineral oil, 40% alkyl polyglucoside, and 40% alcohol ethoxylate containing five ethylene oxide units.
3VORTEXX ™ commercial peracid (Ecolab)
41000 ppm monoisopropanol amine salt of linear alkylbenzene sulfonate.
The results in Table VI show that completely emulsifying the solvent system into a single phase using a surfactant can reduce antimicrobial efficacy, as can be seen by comparing Run Nos. 6-1 and 6-2. Conversely, use of a surfactant that can partially solubilize (or even destabilize) the composition can improve antimicrobial efficacy, as can be seen by comparing Run Nos. 6-3 and 6-4. - Using the method of Example 2, varying amounts of sparingly soluble solvent blends were added to a peracid bottle washing formulation (15C; Ecolab) and tested against spores of Bacillus subtilis and the mold C. funicola using a 10 second contact time at 60° C. Set out below in Table VII are the run number, solvents, solvent concentrations, peracid concentration, appearance of the mixtures after they had been allowed to stand for 1 minute, and the observed log order reduction for Bacillus cereus and C. funicola for each composition.
TABLE VII Run Peracid2 Log Reduction No. Solvent(s) Solvent (%) (ppm) Appearance B. subtilis C. funicola 7-1 Diester blend2 2.5% 1500 hazy >6.5 >4.4 7-2 Benzyl alcohol 3.5% 1000 hazy 6.1 5.2 7-3 Diester blend,2/ 1.5/1.0% 0 clear 0 >4.4 benzyl alcohol 7-4 Diester blend,2/ 1.0/1.5% 0 clear 0 >4.4 benzyl alcohol 7-5 Diester blend,2/ 1.0/1.5% 1500 clear >6.0 >4.4 benzyl alcohol 7-6 Diester blend,2/ 1.5/1.0% 1500 clear >6.0 >4.4 benzyl alcohol
115C commercial peracid (Ecolab)
2DBE-3 ™ (DuPont Nylon)
The results in Table VII show substantial enhancement in antimicrobial efficacy for compositions both above and below the water solubility limit (as evidenced visually by solution clarity) of the antimicrobially-active solvent. Most notable are the blended solvent systems shown in Run Nos. 7-5 and 7-6, which utilized each solvent below its solubility limit and a peracid, and provided significant broad-spectrum antimicrobial efficacy using clear solutions. - Using the method of Example 2, a sparingly soluble solvent was added to various additional antimicrobial agents and tested against Bacillus cereus, Bacillus subtilis, C. funicola and N. fisheri using a 10 second contact time at 60° C. Set out below in Table VIII are the run number, solvent and antimicrobial agent employed, solvent amount, antimicrobial agent amount, and the observed log order reduction for Bacillus cereus, Bacillus cereus, C. funicola, or N. fisheri for each composition.
TABLE VIII Run Solvent + Additional Solvent Additional Log Reduction No. Antimicrobial Agent(s) Amount Agent Amount B. cereus B. subtilis C. funicola N. fisheri 8-1 Diester blend,1 + 2.5% 200 ppm >6.3 >6.0 >4.4 >4.6 NaOCl5 8-2 Diester blend,1 + 2.5% 400 ppm — >6.0 >4.4 — NaOCl5 8-32 Diester blend,1 + 5.0% 2.1% — 2.9 >4.4 2.1 H2O2 8-42 Diester blend,1 + 5.0% .2% — >6.0 >4.4 — H2O2 8-5 Diester blend,1 + 2.5% 800 + 1500 ppm — — >4.8 — C8FA3 + POAA4 8-6 Diester blend,1 1.5% 0 — — 0.2 — 8-7 C8FA3 800 ppm 0 — — 0.2 — 8-8 POAA4 1500 ppm 0 — — 0.2 —
1DBE-3 ™ (Dupont Nylon)
2The solution was aged >18 hours prior to use.
3C8FA = octanoic acid.
4POAA = peroxyacetic acid.
5Acidified to pH = 6.0 with acetic acid.
The results in Table VIII illustrate use of various combinations of solvents and additional antimicrobial agents in the present invention. The mixture shown in Run No. 8-5 gave an especially synergistic result compared to the three control compositions of Run Nos. 8-6 through 8-8. - Using the method of Example 2, a composition was tested against the spore Bacillus cereus and the mold C. funicola using a 120 second contact time at 40° C. These experiments were run in order to determine the antimicrobial effectiveness of a composition of the present invention at a lower treatment temperature. Set out below in Table IX are the run number, solvent and additional antimicrobial agent, solvent amount, additional antimicrobial agent amount, and the observed log order reduction for Bacillus cereus and C. funicola for each composition.
TABLE IX Solvent + Additional Additional Log Reduction Run No. Antimicrobial Agent Solvent (%) Agent Amount B. cereus C. funicola 9-1 Diester blend,1 + NaOCl 2.5% 200 ppm >6.3 3.2 9-22 Diester blend,1 + H2O2 3.0% 0.84% >6.3 1.0 9-32 Diester blend,1 + H2O2 2.5% .70% >6.3 1.0 9-4 Diester blend,1 + POAA3 2.5% 1500 ppm >6.3 2.7
1DBE-3 ™ (Dupont Nylon)
2The solution was aged >18 hours prior to use.
3Peroxyacetic acid
The results in Table IX demonstrate the ability to induce effective microbial control at lower treatment temperatures. - Aqueous mixtures containing an antimicrobially-active solvent, a peracid, or mixtures of both were prepared and evaluated against the spore-forming, enterotoxin producing pathogen Bacillus cereus using a 10 second contact time at 60° C. Set out below in Table X are the run number, solvent, solvent concentration, peracid concentration, and the observed log order reduction for B. cereus for each composition.
TABLE X Run Solvent, Peracid B. cereus No. Solvent (wt %) (ppm) Log Reduction 10-1 None 0% 10001 0.2 10-2 None 0% 30001 0.9 10-3 None 0% 40002 0.8 10-4 Benzyl alcohol 3% 0 0.1 10-5 Benzyl alcohol 3% 10001 2.4 10-6 Diester blend,3 3% 0 0.3 10-7 Diester blend,3 3% 10002 3.6 10-8 Diester blend,3 2.5% 15004 >6.3
1OXONIA ACTIVE ™ commercial peracid (Ecolab)
2MATRIXX ™ commercial peracid (Ecolab)
315C commercial peracid (Ecolab)
4DBE-3 ™ (Dupont Nylon)
The results in Table X show the substantial synergistic improvements in sporicidal efficacy that can be obtained by combining the antimicrobially-active solvent and a peracid, as can be seen by comparing Run Nos. 10-1, 10-4 and 10-5, and Run Nos. 10-3, 10-6 and 10-7. Run No. 10-7 provided nearly a 3-log reduction improvement compared to the use of the antimicrobially-active solvent or peracid alone, while using a lower quantity of peracid. Run No. 10-8 provided an especially effective sporicide at even lower levels of antimicrobially-active solvent and peracid. - Using the method of Example 10, aqueous mixtures containing 3% benzyl alcohol, or varying amounts of several peracids (KX-6091, MATRIXX™, TSUNAMI 100™ or OXONIA ACTIVE™; Ecolab), or mixtures of both benzyl alcohol and peracid were prepared and evaluated as possible sterilant formulations against the spore-forming, enterotoxin producing pathogen Bacillus cereus using a 10 second contact time at 60° C. Set out below in Table VIII are the run number, solvent, peracid concentration, and the observed log order reduction for B. cereus for each composition.
TABLE XI Run Solvent, Peracid, B. cereus No. Solvent (wt %) (ppm) Log Reduction 11-1 Benzyl alcohol 3% 10001 >6.5 11-2 Benzyl alcohol 3% 10002 >6.5 11-3 Benzyl alcohol 3% 10003 >5.6 11-4 Benzyl alcohol 3% 10004 2.4 11-5 Benzyl alcohol 3% 10005 >6.3 11-5 Benzyl alcohol 3% None 0.1 11-6 None 0% 40004 0.8 11-7 None 0% 40002 0.8
1KX-6091 commercial peracid (Ecolab)
2MATRIXX ™, mixed peracid (Ecolab)
3TSUNAMI-100 ™ commercial peracid (Ecolab)
4OXONIA ACTIVE ™ commercial peracid (Ecolab)
515C commercial peracid (Ecolab)
The results in Table XI show the substantial synergistic improvements in sporicidal efficacy that can be obtained by combining the antimicrobially-active solvent and a peracid. For example, Run No. 11-2 provided more than a 6-log reduction improvement compared to the use of the antimicrobially-active solvent alone (Run No. 11-5), and nearly a 6-log reduction improvement compared to the use of the peracid alone (Run No. 11-7), yet required only one-fourth as much peracid. - Using the method of Example 10, aqueous mixtures containing various solvents and varying amounts of a peracid (15C; Ecolab) were prepared and evaluated against the spore-forming, enterotoxin producing pathogen Bacillus cereus using a 10 second contact time at 60° C. Set out below in Table XII are the run number, solvent type and concentration, peracid type and concentration, and the observed log order reduction for B. cereus for each composition. As shown, a wide range of chemical solvent classes yielded substantial spore reductions.
TABLE XII Run Solvent Type and Peracid Type and - B. cereus No. Amount (wt %) Amount (ppm) Log Reduction 12-1 Phenoxyethanol (2.5%) 15C1 (1200 ppm) 3.4 12-2 Phenethanol (3.0%) 15C1 (2000 ppm) >6.4 12-3 Benzoic acid (0.5%) 15C1 (2000 ppm) >6.0 12-4 Benzyl benzoate (0.5%) 15C1 (2000 ppm) 2.3 12-5 Diester blend,2 (2.5%) 15C1 (1200 ppm) >6.2
115C commercial peracid (Ecolab)
2DBE-3 ™ (Dupont Nylon)
- Using the method of Example 10, aqueous mixtures containing varying types and amounts of solvents and varying types and amounts of several peracids were prepared and evaluated as sporicides against Bacillus subtilis, using a 10 second contact time at 60° C. Set out below in Table XIII are the run number, solvent, solvent concentration, peracid type and concentration, and the observed log order reduction for B. subtilis for each composition.
TABLE XIII B. subtilis Run Solvent Peracid Type and Log No. Solvent wt % Amount, ppm Reduction 13-1 Benzyl alcohol 3.0% VORTEXX ™,1 >6.7 (1000 ppm) 13-2 Benzyl alcohol 3.0% VORTEXX ™,1 >6.7 (1500 ppm) 13-3 Benzyl alcohol 3.5% VORTEXX ™,1 >6.7 (1000 ppm) 13-4 Benzyl alcohol 3.0% VORTEXX ™,1 >6.7 (1500 ppm) 13-5 Benzyl alcohol 3.5% 15C2 (1000 ppm) 5.6 13-6 Benzyl alcohol 2.5% VORTEXX ™,1 >6.7 (1500 ppm) 13-7 Benzyl alcohol 2.0% VORTEXX ™,1 >6.7 (1500 ppm) 13-84 Benzyl alcohol 2.0% TSUNAMI 100 ™,3 >6.7 (1000 ppm) 13-9 Phenoxyethanol 5.0% 15C2 (1000 ppm) 6.7 13-10 Phenoxyethanol 5.0% VORTEXX ™,1 6.7 (1000 ppm) 13-11 Phenoxyethanol 2.5% 15C2 (1500 ppm) >6.5 13-12 Phenoxyethanol- 5.0% 15C2 (1000 ppm) 6.7 tetra ethoxylate 13-13 Diester blend5 2.5% 15C2 (1500 ppm) >6.5 13-14 Diester blend5 1.5% VORTEXX ™,1 >6.7 (1500 ppm) 13-15 Tert-butanol 5.0% VORTEXX ™,1 >6.7 (1500 ppm)
1VORTEXX ™, mixed peracid (Ecolab)
215C commercial peracid (Ecolab)
3TSUNAMI 100 ™, commercial peracid (Ecolab)
4Also contained 1000 ppm sodium octyl sulfonate
5DBE-3 ™ (Dupont Nylon)
- Using the method of Example 2, aqueous mixtures containing 2.5 wt. % DBE-3™solvent (diester blend, DuPont Nylon) and a peracid were prepared and evaluated as a general antimicrobial agent against S. aureus, E. coli, or N. fisheri using a 10 second contact time at 60° C. Set out below in Table XIV are the run number, peracid type and amount, and the observed log order reduction for each organism.
TABLE XIII Run Peracid Type and Log Reduction No. Amount (ppm) S. aureus E. coli N. fisheri 14-1 15C1 (2000 ppm) — — 3.7 14-2 OXONIA ACTIVE ™,2 >7.2 >7.1 — (75 ppm)
1Commercial peracid (Ecolab)
2Commercial peracid (Ecolab)
- Various modifications and alterations of this invention will be apparent to those skilled in the art without departing from the scope and spirit of this invention. It should be understood that this invention is not limited to the illustrative embodiments set forth above.
Claims (33)
1-49. (canceled)
50. A method of reducing the population of a microbe comprising:
applying to microbes a composition comprising:
diluting solvent;
antimicrobially-active solvent having a density different from the density of the diluting solvent and comprising ether, hydroxyether, C1-6 protonated carboxylic acid, or mixture thereof;
additional antimicrobial agent comprising carboxylic acid, active oxygen compound, or mixture thereof; and
optional cosolvent, surfactant, or mixture thereof.
51. The method of claim 50 , wherein the amount of antimicrobially-active solvent or additional antimicrobial agent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition will provide greater than a I-log order reduction in the population of spores or bacteria of Bacillus cereus within 10 seconds at 60° C.
52. The method of claim 50 , wherein the diluting solvent comprises water.
53. The method of claim 50 , wherein the antimicrobially active solvent comprises ethylene glycol phenyl ether, propylene glycol phenyl ether 2-ethyl-1-hexanoic acid, butyric acid, octanoic acid, heptanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
54. The method of claim 53 , wherein the antimicrobially active solvent comprises 2-ethyl-1-hexanoic acid, butyric acid, octanoic acid, heptanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
55. The method of claim 50 , wherein the additional antimicrobial agent comprises butyric acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
56. The method of claim 50 , wherein the additional antimicrobial agent comprises peracid.
57. The method of claim 56 , wherein the additional antimicrobial agent comprises peracetic acid, perheptanoic acid, peroctanoic acid, perdecanoic acid, or mixture thereof.
58. The method of claim 50 , wherein the composition comprises surfactant and the surfactant comprises anionic surfactant.
59. The method of claim 58 , wherein the anionic surfactant comprises C6-C24 alkylbenzene sulfonates, C6-C24 olefin sulfonates, C6-C24 paraffin sulfonates, cumene sulfonate, xylene sulfonate, C6-C24 alkyl naphthalene sulfonates, C6-C24 alkyl or dialkyl diphenyl ether sulfonates or disulfonates, C4-C24 mono or dialkyl sulfosuccinates, sulfonated or sulfated fatty acids, C6-C24 alcohol sulfates, C6-C24 alcohol ether sulfates having 1 to about 20 ethylene oxide groups C4-C24 alkyl, aryl or alkaryl phosphate esters or their alkoxylated analogues having 1 to about 40 ethylene, propylene or butylene oxide units, or mixture thereof.
60. The method of claim 50 , comprising applying the composition to a hard surface.
61. An antimicrobial concentrate and instructions for mixing the concentrate with water,
the concentrate comprising:
antimicrobially-active solvent having a density different from the density of water and comprising ether, hydroxyether, C1-16 protonated carboxylic acid, or mixture thereof;
additional antimicrobial agent comprising carboxylic acid, active oxygen compound, or mixture thereof, and
optional cosolvent, surfactant, or mixture thereof.
62. The composition of claim 61 , wherein the amount of antimicrobially-active solvent or additional antimicrobial agent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of spores or bacteria of Bacillus cereus within 10 seconds at 60° C.
63. The composition of claim 61 , wherein the antimicrobially active solvent comprises 2-ethyl-1-hexanoic acid, butyric acid, octanoic acid, heptanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
64. The composition of claim 61 , wherein the additional antimicrobial agent comprises butyric acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
65. The composition of claim 61 , wherein the additional antimicrobial agent comprises peracid.
66. The composition of claim 65 , wherein the additional antimicrobial agent comprises peracetic acid, perheptanoic acid, peroctanoic acid, perdecanoic acid, or mixture thereof.
67. The composition of claim 61 , the composition comprising surfactant and the surfactant comprising anionic surfactant.
68. The composition of claim 67 , wherein the anionic surfactant comprises C6-C24 alkylbenzene sulfonates, C6-C24 olefin sulfonates, C6-C24 paraffin sulfonates, cumene sulfonate, xylene sulfonate, C6-C24 alkyl naphthalene sulfonates, C6-C24 alkyl or dialkyl diphenyl ether sulfonates or disulfonates, C4-C24 mono or dialkyl sulfosuccinates, sulfonated or sulfated fatty acids, C6-C24 alcohol sulfates, C6-C24 alcohol ether sulfates having 1 to about 20 ethylene oxide groups, C4-C24 alky, aryl or alkaryl phosphate esters or their alkoxylated analogues having 1 to about 40 ethylene, propylene or butylene oxide units, or mixture thereof.
69. The composition of claim 61 , wherein the amount of antimicrobially-active solvent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that a clear single-phase solution or microemulsion is not formed when the concentrate is mixed with water according to the instructions.
70. An antimicrobial composition comprising:
diluting solvent;
antimicrobially-active solvent having a density different from the density of the diluting solvent and comprising ether, hydroxyether, C1-16 protonated carboxylic acid, or mixture thereof;
additional antimicrobial agent comprising carboxylic acid, active oxygen compound, or mixture thereof; and
optional cosolvent, surfactant, or mixture thereof.
71. The composition of claim 70 , wherein the amount of antimicrobially-active solvent or additional antimicrobial agent is sufficiently high and the amount of cosolvent or surfactant is sufficiently low so that the composition will provide greater than a 1-log order reduction in the population of spores or bacteria of Bacillus cereus within 10 seconds at 60° C.
72. The composition of claim 70 , wherein the diluting solvent comprises water.
73. The composition of claim 70 , wherein the antimicrobially active solvent comprises 2-ethyl-1-hexanoic acid, butyric acid, octanoic acid, heptanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
74. The composition of claim 70 , wherein the additional antimicrobial agent comprises butyric acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, or mixture thereof.
75. The composition of claim 70 , wherein the additional antimicrobial agent comprises peracid.
76. The composition of claim 75 , wherein the additional antimicrobial agent comprises peracetic acid, perheptanoic acid, peroctanoic acid, perdecanoic acid, or mixture thereof.
77. The composition of claim 70 , the composition comprising surfactant and the surfactant comprising anionic surfactant.
78. The composition of claim 77 , wherein the anionic surfactant comprises C6-C24 alkylbenzene sulfonates, C6-C24 olefin sulfonates, C6-C24 paraffin sulfonates, cumene sulfonate, xylene sulfonate, C6-C24 alkyl naphthalene sulfonates, C6-C24 alkyl or dialkyl diphenyl ether sulfonates or disulfonates, C4-C24 mono or dialkyl sulfosuccinates, sulfonated or sulfated fatty acids, C6-C24 alcohol sulfates, C6-C24 alcohol ether sulfates having 1 to about 20 ethylene oxide groups, C4-C24 alkyl, aryl or alkaryl phosphate esters or their alkoxylated analogues having 1 to about 40 ethylene, propylene or butylene oxide units, or mixture thereof.
79. The composition of claim 70 , wherein the composition exhibits pseudo-stable phase-splitting behavior.
80. The composition of claim 70 , wherein the composition exhibits quasi-stable behavior.
81. The composition of claim 70 , wherein the composition is not a clear single-phase solution or microemulsion.
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
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US20100159028A1 (en) * | 2008-12-18 | 2010-06-24 | Minntech Corporation | Sporicidal hand sanitizing lotion |
US20100249166A1 (en) * | 2007-09-19 | 2010-09-30 | Xy, Inc. | Differential evaporation potentiated disinfectant system |
US20160102275A1 (en) * | 2008-02-11 | 2016-04-14 | Ecolab Usa Inc. | Use of activator complexes to enhance lower temperature cleaning in alkaline peroxide cleaning systems |
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Families Citing this family (133)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6010729A (en) | 1998-08-20 | 2000-01-04 | Ecolab Inc. | Treatment of animal carcasses |
WO2001082694A1 (en) | 2000-04-28 | 2001-11-08 | Ecolab Inc. | Antimicrobial composition |
US20030180377A1 (en) | 2002-02-12 | 2003-09-25 | Ramirez Jose A. | Enhanced activity hydrogen peroxide disinfectant |
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US9072294B2 (en) * | 2006-02-15 | 2015-07-07 | Cognis Ip Management Gmbh | Emulsifier system and pesticidal formulations containing the emulsifier system |
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EP2120561A4 (en) | 2007-02-19 | 2012-11-21 | Plurogen Therapeutics Inc | Compositions for treating biofilms and methods for using same |
EP2182811A4 (en) * | 2007-08-15 | 2012-11-28 | Virox Technologies Inc | Antimicrobial compositions |
US9271494B2 (en) * | 2007-08-30 | 2016-03-01 | Ecolab USA, Inc. | Shelf stable, reduced corrosion, ready to use peroxycarboxylic acid antimicrobial compositions |
US7828885B1 (en) * | 2008-01-17 | 2010-11-09 | World-Pharm Trust | Anti-microbial compositions and polymers |
EP2133102B1 (en) * | 2008-03-19 | 2014-12-03 | Symrise AG | Odor reducers |
PE20100026A1 (en) * | 2008-03-25 | 2010-02-06 | Albemarle Corp | CONTAINERS AND METHODS TO REDUCE OR ELIMINATE BACTERIA IN EGGS FOR HUMAN CONSUMPTION |
WO2010008899A2 (en) * | 2008-06-24 | 2010-01-21 | Fresh Express Incorporated | Peracid and 2-hydroxy organic acid compositions and methods for treating produce |
BRPI0914184A2 (en) | 2008-09-30 | 2015-10-20 | Virox Technologies Inc | concentrated hydrogen peroxide disinfectant solutions. |
JP5463026B2 (en) * | 2008-12-11 | 2014-04-09 | 花王株式会社 | Antibacterial composition |
FR2941462B1 (en) * | 2009-01-23 | 2013-07-05 | Rhodia Operations | STRIPPING COMPOSITION |
EP3205344A1 (en) | 2009-05-19 | 2017-08-16 | Plurogen Therapeutics, LLC | Surface active agent compositions and methods for enhancing oxygenation, reducing bacteria and improving wound healing |
EP3480132A1 (en) | 2009-05-28 | 2019-05-08 | Ecolab USA Inc. | Wetting agents for aseptic filling |
WO2011079079A1 (en) * | 2009-12-21 | 2011-06-30 | Fresh Express Incorporated | Peracid and 2-hydroxy organic acid compositions and methods for treating items |
JP2013515072A (en) * | 2009-12-21 | 2013-05-02 | フレッシュ・エクスプレス・インコーポレイテッド | Sterilization of articles with peracid and 2-hydroxy organic acid compositions |
US8394751B2 (en) * | 2010-01-29 | 2013-03-12 | W. M. Barr & Company | Organic residue remover composition |
US10563153B2 (en) | 2010-05-20 | 2020-02-18 | Ecolab Usa Inc. | Rheology modified low foaming liquid antimicrobial compositions and methods of use thereof |
US8389457B2 (en) | 2010-09-22 | 2013-03-05 | Ecolab Usa Inc. | Quaternary functionalized alkyl polyglucosides for enhanced food soil removal |
US8329633B2 (en) | 2010-09-22 | 2012-12-11 | Ecolab Usa Inc. | Poly quaternary functionalized alkyl polyglucosides for enhanced food soil removal |
US8658584B2 (en) | 2010-06-21 | 2014-02-25 | Ecolab Usa Inc. | Sulfosuccinate functionalized alkyl polyglucosides for enhanced food and oily soil removal |
US20110312867A1 (en) | 2010-06-21 | 2011-12-22 | Ecolab Usa Inc. | Betaine functionalized alkyl polyglucosides for enhanced food soil removal |
US20110312866A1 (en) | 2010-06-21 | 2011-12-22 | Ecolab Usa Inc. | Alkyl polypentosides and alkyl polyglucosides (c8-c11) used for enhanced food soil removal |
US20120046215A1 (en) | 2010-08-23 | 2012-02-23 | Ecolab Usa Inc. | Poly sulfonate functionalized alkyl polyglucosides for enhanced food soil removal |
US20120046208A1 (en) | 2010-08-23 | 2012-02-23 | Ecolab Usa Inc. | Poly phosphate functionalized alkyl polyglucosides for enhanced food soil removal |
KR101527105B1 (en) * | 2010-07-09 | 2015-06-08 | 가켄 테크 가부시키가이샤 | Liquid concentrate for cleaning composition, cleaning composition and cleaning method |
JP6215511B2 (en) * | 2010-07-16 | 2017-10-18 | 栗田工業株式会社 | Anticorrosive for boiler |
US8460477B2 (en) | 2010-08-23 | 2013-06-11 | Ecolab Usa Inc. | Ethoxylated alcohol and monoethoxylated quaternary amines for enhanced food soil removal |
US8877703B2 (en) | 2010-09-22 | 2014-11-04 | Ecolab Usa Inc. | Stearyl and lauryl dimoniumhydroxy alkyl polyglucosides for enhanced food soil removal |
JP5657318B2 (en) * | 2010-09-27 | 2015-01-21 | 富士フイルム株式会社 | Semiconductor substrate cleaning agent, cleaning method using the same, and semiconductor device manufacturing method |
US9949477B2 (en) * | 2010-12-30 | 2018-04-24 | Kimberly-Clark Worldwide, Inc. | Durable antimicrobial composition |
US20130280392A1 (en) * | 2011-01-17 | 2013-10-24 | Delaval Holding Ab | Process for controlling microorganisms in beverage products |
US9512387B2 (en) * | 2011-02-11 | 2016-12-06 | Dubois Chemicals, Inc. | Cleaning compositions for removing polymeric contaminants from papermaking surfaces |
US8455551B2 (en) | 2011-03-04 | 2013-06-04 | American Sterilizer Company | Broad spectrum disinfectant |
US10085447B2 (en) | 2011-03-11 | 2018-10-02 | Ecolab Usa Inc. | Acidic biofilm remediation |
US8906963B2 (en) | 2011-07-14 | 2014-12-09 | Ecolab Usa Inc | Deodorization of peracids |
US8883848B2 (en) | 2011-07-14 | 2014-11-11 | Ecolab Usa Inc. | Enhanced microbial peracid compositions and methods of use at reduced temperatures in aseptic cleaning |
US8324146B2 (en) * | 2011-08-30 | 2012-12-04 | Zalk Yehuda | Sodium metasilicate based cleaning solutions |
WO2013056901A1 (en) * | 2011-10-18 | 2013-04-25 | Unilever N.V. | Cleansing composition |
AU2012244292B2 (en) * | 2011-11-04 | 2015-03-05 | Bissell Inc. | Enzyme cleaning composition and method of use |
EP2825212B1 (en) | 2012-03-13 | 2018-01-03 | PeroxyChem LLC | Improved sterilization method |
ITCA20120004A1 (en) * | 2012-03-30 | 2012-06-29 | Abdelkrim Harchi | SINGLE DEHYDRATING AND DIAPHANIZING REAGENT FOR HISTOLOGY AND NON-HARMFUL AND NON-TOXIC, BIODEGRADABLE CITOLOGY 88%, LOW VOLATILITY |
US9758716B2 (en) | 2012-07-03 | 2017-09-12 | Raymond J Roccon | Process for treating a wastewater stream produced by hydrocarbon production operations for repurposing as a disinfectant for hydrocarbon production operations |
US9120966B2 (en) | 2012-07-03 | 2015-09-01 | Raymond J Roccon | Process for disinfecting and stabilizing production water using in-situ hypobromous acid generation |
US8871699B2 (en) | 2012-09-13 | 2014-10-28 | Ecolab Usa Inc. | Detergent composition comprising phosphinosuccinic acid adducts and methods of use |
US20140308162A1 (en) | 2013-04-15 | 2014-10-16 | Ecolab Usa Inc. | Peroxycarboxylic acid based sanitizing rinse additives for use in ware washing |
US9752105B2 (en) | 2012-09-13 | 2017-09-05 | Ecolab Usa Inc. | Two step method of cleaning, sanitizing, and rinsing a surface |
US9994799B2 (en) | 2012-09-13 | 2018-06-12 | Ecolab Usa Inc. | Hard surface cleaning compositions comprising phosphinosuccinic acid adducts and methods of use |
US9873854B2 (en) * | 2013-01-16 | 2018-01-23 | Jelmar, Llc | Stain removing solution |
SG11201509578UA (en) | 2013-05-23 | 2015-12-30 | Nature Seal Inc | Antimicrobial wash |
JP6359392B2 (en) * | 2013-11-27 | 2018-07-18 | 花王株式会社 | Disinfectant composition for food processing equipment or cooking utensils |
WO2015104687A1 (en) * | 2014-01-13 | 2015-07-16 | 99 Holding S.A.R.L. | Activated hydrogen peroxide biocide composition |
US20150252310A1 (en) | 2014-03-07 | 2015-09-10 | Ecolab Usa Inc. | Alkyl amides for enhanced food soil removal and asphalt dissolution |
US11946021B2 (en) * | 2014-03-22 | 2024-04-02 | United Laboratories International, Llc | Solvent composition and process for removal of asphalt and other contaminant materials |
US11053464B2 (en) | 2014-03-22 | 2021-07-06 | United Laboratories International, Llc | Solvent composition and process for removal of asphalt and other contaminant materials |
US10993448B2 (en) | 2014-07-25 | 2021-05-04 | Dole Fresh Vegetables, Inc. | Method for sanitizing fresh produce |
CN117142998A (en) | 2014-12-18 | 2023-12-01 | 艺康美国股份有限公司 | Production of peroxyformic acid by polyol formates |
AU2016209327A1 (en) | 2015-01-20 | 2017-08-10 | Plurogen Therapeutics, Llc | Compositions and methods of treating microbes |
BR112017015615B1 (en) | 2015-01-21 | 2022-09-20 | Arch Chemicals, Inc. | METHOD TO TREAT A BODY OF RECREATIONAL WATER WITH A BIOCIDAL COMPOSITION |
KR101699468B1 (en) * | 2015-03-30 | 2017-01-25 | 주식회사 파이토켐텍 | Preservative composition containing PEG-2 phenyl ether as an active ingredient |
AU2016245785B2 (en) * | 2015-04-09 | 2020-07-09 | Isp Investments Llc | Synergistic preservative compositions |
US10017714B2 (en) | 2015-05-19 | 2018-07-10 | Ecolab Usa Inc. | Efficient surfactant system on plastic and all types of ware |
US9765287B2 (en) | 2015-06-03 | 2017-09-19 | Metrex Research Corporation | Stabilized hydrogen peroxide compositions and method of making same |
US10208274B1 (en) | 2015-07-02 | 2019-02-19 | Zee Company | Brewing vessel cleaning composition and related methods of use |
US10308901B2 (en) * | 2015-07-20 | 2019-06-04 | The James Grady Co. Inc. | Kit for adhesive removal on surfaces and methods and devices thereof |
WO2017036977A1 (en) * | 2015-08-28 | 2017-03-09 | Technische Universiteit Eindhoven | Process for cleaning contaminated plastic material for recycling |
US10251971B2 (en) | 2015-09-03 | 2019-04-09 | The Administrators Of The Tulane Educational Fund | Compositions and methods for multipurpose disinfection and sterilization solutions |
MA45743A (en) * | 2016-02-25 | 2019-01-02 | Ecolab Usa Inc | ETHER AMINES FOR BETTER SPORICIDE PERFORMANCE |
JP6904979B2 (en) | 2016-03-01 | 2021-07-21 | エコラボ ユーエスエー インコーポレイティド | Sterilized rinse based on the synergistic effect of quaternary anionic surfactant |
KR20190028712A (en) * | 2016-06-22 | 2019-03-19 | 브리오테크 인코포레이티드 | Inactivation of high resistant infectious microorganisms and proteins by non-buffered hypohalous acid compositions |
US10426162B2 (en) | 2016-08-11 | 2019-10-01 | Ecolab Usa Inc. | Interaction between antimicrobial quaternary compounds and anionic surfactants |
CA3043589A1 (en) | 2016-11-21 | 2018-05-24 | Virox Technologies Inc. | Novel peroxide stabilizers |
KR101858875B1 (en) * | 2016-12-29 | 2018-06-29 | 주식회사 효성 | Antibacterial spandex using organic antibacterial agent |
EP3418368A1 (en) * | 2017-06-21 | 2018-12-26 | The Procter & Gamble Company | Solvent containing hard surface cleaning compositions |
US10450535B2 (en) | 2017-10-18 | 2019-10-22 | Virox Technologies Inc. | Shelf-stable hydrogen peroxide antimicrobial compositions |
JP7334174B2 (en) | 2018-02-14 | 2023-08-28 | エコラボ ユーエスエー インコーポレイティド | Compositions and methods for reducing biofilms and spores from membranes |
WO2020086929A1 (en) | 2018-10-26 | 2020-04-30 | Ecolab Usa Inc. | Synergistic surfactant package for cleaning of food and oily soils |
KR102140537B1 (en) * | 2018-11-01 | 2020-08-03 | 전남대학교산학협력단 | Controlling composition for blight and controlling method for blight using the same |
CN113166590A (en) | 2018-11-07 | 2021-07-23 | 伊士曼性能薄膜有限责任公司 | Tackifying solutions and their use in applying films to substrates |
US10506811B1 (en) | 2018-11-21 | 2019-12-17 | Pur Products LLC | Anti-microbial solution for seeds, crops, livestock and processed foods |
WO2020167933A1 (en) | 2019-02-12 | 2020-08-20 | Alden Medical, Llc | Alcohol-free hydrogen peroxide disinfectant compositions and methods of use thereof |
US11312922B2 (en) | 2019-04-12 | 2022-04-26 | Ecolab Usa Inc. | Antimicrobial multi-purpose cleaner comprising a sulfonic acid-containing surfactant and methods of making and using the same |
WO2020257222A1 (en) | 2019-06-17 | 2020-12-24 | Ecolab Usa Inc. | Textile bleaching and disinfecting using the mixture of hydrophilic and hydrophobic peroxycarboxylic acid composition |
US20210071108A1 (en) | 2019-09-06 | 2021-03-11 | Ecolab Usa Inc. | Concentrated surfactant systems for rinse aid and other applications |
CN112147262B (en) * | 2020-11-02 | 2022-07-08 | 宁夏大学 | Method for identifying cooked fresh and alive Chinese mitten crabs |
Citations (97)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2512640A (en) * | 1949-07-25 | 1950-06-27 | Buffalo Electro Chem Co | Treatment of raw plant tissue |
US3122417A (en) * | 1959-06-03 | 1964-02-25 | Henkel & Cie Gmbh | Stabilizing agent for peroxy-compounds and their solutions |
US3248281A (en) * | 1963-06-26 | 1966-04-26 | Dow Chemical Co | Iodine-peroxide-bisulfate antimicrobial composition |
US3514278A (en) * | 1968-01-08 | 1970-05-26 | Betz Laboratories | Slime control agent and methods of application |
US3867300A (en) * | 1972-08-10 | 1975-02-18 | Carbolabs Inc | Bactericidal composition |
US3895116A (en) * | 1971-11-29 | 1975-07-15 | Eastman Kodak Co | Mixtures of volatile fatty acids having anti-fungal and anti-bacterial activity |
US4191660A (en) * | 1978-02-27 | 1980-03-04 | International Flavors & Fragrances Inc. | Organoleptic uses of 1-(3,3-dimethyl-2-norbornyl)-2-propanone in cationic, anionic and nonionic detergents and soaps |
US4244884A (en) * | 1979-07-12 | 1981-01-13 | The Procter & Gamble Company | Continuous process for making peroxycarboxylic acids |
US4330531A (en) * | 1976-03-26 | 1982-05-18 | Howard Alliger | Germ-killing materials |
US4370199A (en) * | 1981-11-09 | 1983-01-25 | Westvaco Corporation | Enzymatic catalyzed biocide system |
US4501681A (en) * | 1981-12-23 | 1985-02-26 | Colgate-Palmolive Company | Detergent dish-washing composition |
US4517330A (en) * | 1983-03-30 | 1985-05-14 | Rohm And Haas Company | Floor polish composition having improved durability |
US4529534A (en) * | 1982-08-19 | 1985-07-16 | The Procter & Gamble Company | Peroxyacid bleach compositions |
US4592488A (en) * | 1985-05-24 | 1986-06-03 | Simon Gilbert I | Method for the preparation of chemotherapeutic compositions for the treatment of periodontal disease, compositions therefor and use thereof |
US4592787A (en) * | 1984-11-05 | 1986-06-03 | The Dow Chemical Company | Composition useful for stripping photoresist polymers and method |
US4655781A (en) * | 1984-07-02 | 1987-04-07 | The Clorox Company | Stable bleaching compositions |
US4666622A (en) * | 1985-01-03 | 1987-05-19 | Lever Brothers Company | Stable thickened low pH liquid bleaching compositions containing inorganic peroxy compounds |
US4738840A (en) * | 1986-03-03 | 1988-04-19 | Simon Gilbert I | Presurgical sterilization method |
US4802994A (en) * | 1986-07-17 | 1989-02-07 | Nalco Chemical Company | Biocide treatment to control sulfate-reducing bacteria in industrial process waste waters |
US4891073A (en) * | 1987-07-13 | 1990-01-02 | Pennzoil Products Company | Method of treating surface with water-in-oil emulsion composition |
US4900721A (en) * | 1986-06-09 | 1990-02-13 | Henkel Kommanditgesellschaft Auf Aktien | Disinfectants and their use for disinfecting the skin and mucous membrane |
US4906617A (en) * | 1986-09-30 | 1990-03-06 | L'oreal | Pharmaceutical compositions containing saturated aromatic peroxides |
US4908306A (en) * | 1987-06-12 | 1990-03-13 | Life Technologies, Inc. | Human papillomavirus 56 nucleic acid hybridization probes and methods for employing the same |
US4917815A (en) * | 1988-06-10 | 1990-04-17 | Sterling Drug Inc. | Stable aqueous aromatic percarboxylic acid solution |
US4923677A (en) * | 1985-08-07 | 1990-05-08 | Roy T. Witkin | Chemical sterilization |
US4937066A (en) * | 1989-06-22 | 1990-06-26 | David G. Vlock | Zinc containing oral compositions |
US4943414A (en) * | 1987-07-30 | 1990-07-24 | Johnson & Johnson Medical, Inc. | Method for vapor sterilizaton of articles having lumens |
US4945110A (en) * | 1987-06-15 | 1990-07-31 | Quali Tech, Inc. | Membrame-forming veterinary antibacterial teat dip |
US4992108A (en) * | 1990-01-18 | 1991-02-12 | Ward Irl E | Photoresist stripping compositions |
US4996062A (en) * | 1988-10-28 | 1991-02-26 | Stabra Ag | Glucose oxidase food treatment and storage method |
US4997571A (en) * | 1990-01-05 | 1991-03-05 | Mogul Corporation | Method of treating water |
US4997625A (en) * | 1985-08-07 | 1991-03-05 | Simon Gilbert I | Chemical sterilization |
US5004760A (en) * | 1990-02-16 | 1991-04-02 | The Dow Chemical Company | Biocidal foams |
US5007969A (en) * | 1988-05-20 | 1991-04-16 | The Boeing Company | Low toxicity liquid solvent |
US5010109A (en) * | 1988-06-30 | 1991-04-23 | Chisso Corporation | Antimicrobial agent composition |
US5015408A (en) * | 1988-03-19 | 1991-05-14 | Reckitt Gmbh | Denture cleaning tablet containing a bleach activator and an organic phosphonic acid stabilizer |
US5078896A (en) * | 1986-09-29 | 1992-01-07 | Akzo N.V. | Thickened aqueous cleaning compositions |
US5080831A (en) * | 1989-06-29 | 1992-01-14 | Buckeye International, Inc. | Aqueous cleaner/degreaser compositions |
US5080822A (en) * | 1990-04-10 | 1992-01-14 | Buckeye International, Inc. | Aqueous degreaser compositions containing an organic solvent and a solubilizing coupler |
US5084239A (en) * | 1990-08-31 | 1992-01-28 | Abtox, Inc. | Plasma sterilizing process with pulsed antimicrobial agent treatment |
US5093140A (en) * | 1988-07-20 | 1992-03-03 | Eisai Co., Ltd. | Aqueous bactericide for animal treatment |
US5096501A (en) * | 1990-08-27 | 1992-03-17 | E. I. Du Pont De Nemours & Company | Environmentally safe cleaning process and cleaning composition useful therein |
US5114718A (en) * | 1990-09-20 | 1992-05-19 | The Procter & Gamble Company | Sustained release compositions for treating periodontol disease |
US5122538A (en) * | 1990-07-23 | 1992-06-16 | Ecolab Inc. | Peroxy acid generator |
US5130124A (en) * | 1991-05-01 | 1992-07-14 | Isp Investments Inc. | Stabilized, aqueous, film-forming antimicrobial compositions of hydrogen peroxide |
US5129824A (en) * | 1989-12-21 | 1992-07-14 | Keller Duane C | Method for treating periodontal disease |
US5176899A (en) * | 1991-11-25 | 1993-01-05 | Montgomery Robert E | Antimicrobial dentifrice |
US5180749A (en) * | 1989-08-22 | 1993-01-19 | Sterling Winthrop, Inc. | Antimicrobial composition |
US5200189A (en) * | 1991-07-23 | 1993-04-06 | Ecolab Inc. | Peroxyacid antimicrobial composition |
US5208057A (en) * | 1991-11-12 | 1993-05-04 | Rohm And Haas Company | Process for butchering and disinfecting fowl |
US5230821A (en) * | 1991-12-03 | 1993-07-27 | E. I. Du Pont De Nemours And Company | Cleaning composition |
US5292447A (en) * | 1988-06-14 | 1994-03-08 | Ausimont S.R.L. | Heterocyclic peroxides having n-amidic heteroatoms |
US5298222A (en) * | 1989-08-09 | 1994-03-29 | Osteotech, Inc. | Process for disinfecting musculoskeletal tissue and tissues prepared thereby |
US5319018A (en) * | 1991-04-23 | 1994-06-07 | Rohm And Haas Company | Transition metal crosslinking of acid-containing polymers |
US5391324A (en) * | 1991-02-01 | 1995-02-21 | Hoechst Aktiengesellschaft | Aqueous suspensions of peroxycarboxylic acids |
US5409713A (en) * | 1993-03-17 | 1995-04-25 | Ecolab Inc. | Process for inhibition of microbial growth in aqueous transport streams |
US5419908A (en) * | 1991-06-04 | 1995-05-30 | Ecolab Inc. | Sanitizing composition comprising a blend of aromatic and polyunsaturated carboxylic acids |
US5419848A (en) * | 1993-07-02 | 1995-05-30 | Buckeye International, Inc. | Aqueous degreaser emulsion compositions |
US5435808A (en) * | 1993-09-03 | 1995-07-25 | Birko Corporation | Hide raceway treatment and improved method of curing hides |
US5436868A (en) * | 1992-07-07 | 1995-07-25 | Oki Electric Industry Co., Ltd. | Word line selection circuit for selecting memory cells |
US5489434A (en) * | 1991-07-23 | 1996-02-06 | Ecolab Inc. | Peroxyacid antimicrobail composition |
US5494588A (en) * | 1993-08-05 | 1996-02-27 | Nalco Chemical Company | Method and composition for inhibiting growth of microorganisms including peracetic acid and a second organic biocide |
US5494707A (en) * | 1991-09-12 | 1996-02-27 | Mannington Mills, Inc. | Resilient floor covering and method of making same |
US5502148A (en) * | 1993-01-28 | 1996-03-26 | Bayer Aktiengesellschaft | Two-component polyurethane coating compositions and their use for coating or sealing floors |
US5508046A (en) * | 1991-07-15 | 1996-04-16 | Minntech Corporation | Stable, anticorrosive peracetic/peroxide sterilant |
US5512309A (en) * | 1989-02-09 | 1996-04-30 | Rhone-Poulenc Inc. | Process for treating poultry carcasses to increase shelf-life |
US5529887A (en) * | 1993-03-31 | 1996-06-25 | Morton International, Inc. | Water soluble fluoride-containing solution for removing cured photoresist and solder resist mask |
US5591706A (en) * | 1989-08-08 | 1997-01-07 | Akzo Nobel N.V. | Aqueous peroxide compositions with improved safety profile |
US5595967A (en) * | 1995-02-03 | 1997-01-21 | The Procter & Gamble Company | Detergent compositions comprising multiperacid-forming bleach activators |
US5597790A (en) * | 1990-10-22 | 1997-01-28 | The Procter & Gamble Company | Liquid detergent compositions containing a suspended peroxygen bleach |
US5616616A (en) * | 1994-06-01 | 1997-04-01 | Minntech Corporation | Room Temperature sterilant |
US5616335A (en) * | 1993-05-05 | 1997-04-01 | Chemoxal S.A. | Stable thickened disinfecting aqueous composition containing an organic peroxy acid intended for human or animal use |
US5632676A (en) * | 1993-10-12 | 1997-05-27 | Fmc Corporation | Use of peracetic acid to sanitize processed fowl |
US5635492A (en) * | 1990-08-31 | 1997-06-03 | Diversey Lever, Inc. | Teat treating compositions, production and use |
US5637559A (en) * | 1993-03-30 | 1997-06-10 | Minnesota Mining And Manufacturing Company | Floor stripping composition and method |
US5641530A (en) * | 1995-11-27 | 1997-06-24 | Eka Nobel Inc. | Method of disinfection |
US5712239A (en) * | 1996-04-08 | 1998-01-27 | Lever Brothers Company, Division Of Conopco, Inc. | Aqueous liquid compositions comprising peracid compounds and substituted phenolic compounds |
US5744440A (en) * | 1993-03-30 | 1998-04-28 | Minnesota Mining And Manufacturing Company | Hard surface cleaning compositions including a very slightly water-soluble organic solvent |
US5756139A (en) * | 1993-08-03 | 1998-05-26 | Solvay Interox Limited | Egg washing and disinfection process |
US5773487A (en) * | 1991-05-15 | 1998-06-30 | Uv Coatings, Inc. | Finishing composition which is curable by UV light and method of using same |
US5785867A (en) * | 1993-08-05 | 1998-07-28 | Nalco Chemical Company | Method and composition for inhibiting growth of microorganisms including peracetic acid and a non-oxidizing biocide |
US5891392A (en) * | 1996-11-12 | 1999-04-06 | Reckitt & Colman Inc. | Ready to use aqueous hard surface cleaning and disinfecting compositions containing hydrogen peroxide |
US5900256A (en) * | 1996-09-18 | 1999-05-04 | Cottrell, Ltd. | Hydrogen peroxide disinfecting and sterilizing compositions |
US5902619A (en) * | 1994-12-02 | 1999-05-11 | Rubow; Ulrik | Method and apparatus for disinfecting or sterilizing foodstuffs and other articles |
US6010995A (en) * | 1995-12-28 | 2000-01-04 | Buckeye International, Inc. | No/low volatile organic compound cleaner/degreaser composition |
US6010729A (en) * | 1998-08-20 | 2000-01-04 | Ecolab Inc. | Treatment of animal carcasses |
US6022551A (en) * | 1998-01-20 | 2000-02-08 | Ethicon, Inc. | Antimicrobial composition |
US6024986A (en) * | 1999-05-24 | 2000-02-15 | Ecolab Inc. | Method of protecting growing plants from the effects of plant pathogens |
US6028104A (en) * | 1997-01-30 | 2000-02-22 | Ecolab Inc. | Use of peroxygen compounds in the control of hairy wart disease |
US6033705A (en) * | 1998-07-08 | 2000-03-07 | Isaacs; Charles E. | Method for treating foodstuffs to reduce or prevent microbial activity |
US6049002A (en) * | 1994-03-09 | 2000-04-11 | Kemira Chemicals B.V. | Method for the preparation of aqueous solutions containing performic acid as well as their use |
US6080416A (en) * | 1998-01-20 | 2000-06-27 | Ethicon, Inc. | Low tack lotion, gels and creams |
US6238685B1 (en) * | 1998-04-06 | 2001-05-29 | Ecolab Inc. | Peroxy acid treatment to control pathogenic organisms on growing plants |
US6399689B1 (en) * | 1999-03-03 | 2002-06-04 | Lilly Industries, Inc. | Abrasion resistant coatings |
US6506261B1 (en) * | 1999-06-24 | 2003-01-14 | Ecolab Inc. | Detergent compositions for the removal of complex organic or greasy soils |
US6544942B1 (en) * | 2000-04-28 | 2003-04-08 | Ecolab Inc. | Phase-separating solvent composition |
US6998369B2 (en) * | 2000-04-28 | 2006-02-14 | Ecolab Inc. | Antimicrobial composition |
Family Cites Families (96)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3350265A (en) | 1966-01-04 | 1967-10-31 | Grace W R & Co | Aqueous polymeric latex with silver and sodium hypochlorite, hydrogen peroxide, or formaldehyde germicides |
US3996386A (en) | 1971-12-15 | 1976-12-07 | Yrjo Malkki | Method for preventing microbial surface deterioration of foods and feeds |
DE2365172A1 (en) | 1973-12-29 | 1975-07-10 | Hoechst Ag | BLEACHING AGENTS FOR DETERGENTS AND DETERGENTS |
NL7608265A (en) | 1975-08-16 | 1977-02-18 | Henkel & Cie Gmbh | STABLE CONCENTRATES OF FUNCTIONAL RESOURCES IN STORAGE. |
NL7608266A (en) | 1975-08-16 | 1977-02-18 | Henkel & Cie Gmbh | CONCENTRATES OF MICROBICIDE AGENTS. |
US4130435A (en) * | 1975-09-18 | 1978-12-19 | E. I. Du Pont De Nemours And Company | Process for preparing a ball-point pen ink |
FR2324626A1 (en) | 1975-09-19 | 1977-04-15 | Anvar | Percarboxylic acids prepn. from acid chlorides in soln. - using conc. hydrogen peroxide, opt. with pyridine |
US4041149A (en) | 1976-01-12 | 1977-08-09 | Colgate-Palmolive Company | Composition and method of controlling and preventing mouth odor |
DE2654164C2 (en) | 1976-11-30 | 1978-08-10 | Schuelke & Mayr Gmbh, 2000 Norderstedt | Aqueous perglutaric acid solution and its use |
DE2725067A1 (en) | 1977-06-03 | 1978-12-14 | Schuelke & Mayr Gmbh | ALCOHOLIC DISINFECTANT WITH SPORICIDAL EFFECT |
CA1146851A (en) | 1978-05-01 | 1983-05-24 | Donald F. Greene | Hydrogen peroxide disinfecting and sterilizing compositions |
US4414128A (en) * | 1981-06-08 | 1983-11-08 | The Procter & Gamble Company | Liquid detergent compositions |
US4404040A (en) * | 1981-07-01 | 1983-09-13 | Economics Laboratory, Inc. | Short chain fatty acid sanitizing composition and methods |
US4421782A (en) * | 1981-10-26 | 1983-12-20 | Armstrong World Industries, Inc. | Process for providing improved radiation-curable surface coverings and products produced thereby |
US4478683A (en) | 1981-11-09 | 1984-10-23 | Westvaco Corporation | Enzymatic catalyzed biocide system |
US4477438A (en) | 1982-11-12 | 1984-10-16 | Surgikos, Inc. | Hydrogen peroxide composition |
GB8328654D0 (en) | 1983-10-26 | 1983-11-30 | Interox Chemicals Ltd | Hydrogen peroxide compositions |
US4689168A (en) * | 1984-06-08 | 1987-08-25 | The Drackett Company | Hard surface cleaning composition |
GB8506735D0 (en) | 1985-03-15 | 1985-04-17 | Diversey Corp | Sanitising & rinsing process |
DE3543500A1 (en) | 1985-12-10 | 1987-06-11 | Schuelke & Mayr Gmbh | Aqueous solution of aromatic percarboxylic acids and its use |
GB8603960D0 (en) | 1986-02-18 | 1986-03-26 | Interox Chemicals Ltd | Disinfection process |
US4715980A (en) | 1986-03-17 | 1987-12-29 | Diversey Wyandotte Corporation | Antimicrobial sanitizing composition containing n-alkyl and n-alkenyl succinic acid and methods for use |
DE3619375A1 (en) * | 1986-06-09 | 1987-12-10 | Henkel Kgaa | USE OF ALKYLGLYCOSIDES AS A POTENTIZING AGENT IN ANTISEPTIC AGENTS CONTAINING ALCOHOLIC OR CARBONIC ACID, AND DISINFECTING AND CLEANING AGENTS CONTAINING ALKOHOLIC OR CARBONIC ACID WITH REINFORCED BACTICIDE |
DE3642569A1 (en) | 1986-12-12 | 1988-06-23 | Utz Ag Georg | RECOVERABLE FLOOR COVERING |
US5656302A (en) | 1987-05-14 | 1997-08-12 | Minntech Corporation | Stable, shippable, peroxy-containing microbicide |
DE3836622C1 (en) | 1987-10-31 | 1989-06-08 | Harry 5608 Radevormwald De Post | |
PT89876A (en) | 1988-02-29 | 1989-10-04 | Metchem Pty Ltd | NEW METHOD FOR DECORATING AND PROTECTIVE COATING OF FLOOR SURFACES |
FR2639233B1 (en) | 1988-11-23 | 1994-05-06 | Air Liquide | HEMODIALYSIS HYGIENE AGENT |
US4865752A (en) | 1988-12-05 | 1989-09-12 | Jacobs Albert L | Separation and filtration membranes and their regeneration |
US4923077A (en) | 1989-02-14 | 1990-05-08 | Pymah Corporation | Modular heat sink package |
DE3906044A1 (en) | 1989-02-27 | 1990-08-30 | Henkel Kgaa | BLEACHING LIQUID DETERGENT |
US5114178A (en) | 1989-03-13 | 1992-05-19 | Baxter David A | Suspension apparatus |
US5149463A (en) | 1989-04-21 | 1992-09-22 | The Clorox Company | Thickened acidic liquid composition with sulfonate fwa useful as a bleaching agent vehicle |
JP2661745B2 (en) * | 1989-05-02 | 1997-10-08 | 株式会社片山化学工業研究所 | Industrial disinfectant and disinfection method |
US5158710A (en) * | 1989-06-29 | 1992-10-27 | Buckeye International, Inc. | Aqueous cleaner/degreaser microemulsion compositions |
US5145596A (en) * | 1989-08-07 | 1992-09-08 | Dow Corning Corporation | Antimicrobial rinse cycle additive |
US5139788A (en) | 1989-10-17 | 1992-08-18 | Ecolab Inc. | Noncontaminating antimicrobial composition |
CA2033637A1 (en) | 1990-01-08 | 1991-07-09 | Levi J. Cottington | Radiation curable abrasion resistant cyclic ether acrylate coating compositions |
US5069286A (en) | 1990-04-30 | 1991-12-03 | The Mogul Corporation | Method for prevention of well fouling |
EP0461700A1 (en) | 1990-06-12 | 1991-12-18 | Akzo Nobel N.V. | Aqueous disinfectant compositions, and concentrates for preparing the same |
US5043176A (en) | 1990-06-13 | 1991-08-27 | Haarmann & Reimer Corp. | Synergistic antimicrobial compositions |
GB9109929D0 (en) | 1991-05-08 | 1991-07-03 | Interox Chemicals Ltd | Thickened compositions |
WO1992020719A1 (en) | 1991-05-15 | 1992-11-26 | Sokol Andrew A | Finishing composition which is curable by uv light and method of using same |
US5234719A (en) | 1991-06-04 | 1993-08-10 | Ecolab Inc. | Food additive sanitizing compositions |
US5830937A (en) | 1992-02-04 | 1998-11-03 | Congoleum Corporation | Coating and wearlayer compositions for surface coverings |
US5268003A (en) | 1992-03-31 | 1993-12-07 | Lever Brothers Company, Division Of Conopco, Inc. | Stable amido peroxycarboxylic acids for bleaching |
EP0569066B1 (en) | 1992-04-16 | 1995-10-25 | Unilever N.V. | Disinfectant compositions |
US5234703A (en) | 1992-10-31 | 1993-08-10 | Guthery B Eugene | Disinfecting product and process |
US5342551A (en) | 1992-11-04 | 1994-08-30 | Cello Corporation | Noncaustic floor finish remover |
US5436008A (en) * | 1992-12-11 | 1995-07-25 | Ecolab Inc. | Sanitizing compositions |
JP3284643B2 (en) * | 1993-01-25 | 2002-05-20 | 栗田工業株式会社 | Fungicide composition |
US5683724A (en) | 1993-03-17 | 1997-11-04 | Ecolab Inc. | Automated process for inhibition of microbial growth in aqueous food transport or process streams |
WO1994023012A1 (en) * | 1993-04-02 | 1994-10-13 | The Dow Chemical Company | Microemulsion and emulsion cleaning compositions |
US5658467A (en) | 1993-08-05 | 1997-08-19 | Nalco Chemical Company | Method and composition for inhibiting growth of microorganisms including peracetic acid and a non-oxidizing biocide |
US5444094A (en) * | 1993-08-24 | 1995-08-22 | Stepan Company | Methods and compositions for disinfecting surfaces containing tuberculosis causing bacteria |
US5567444A (en) * | 1993-08-30 | 1996-10-22 | Ecolab Inc. | Potentiated aqueous ozone cleaning and sanitizing composition for removal of a contaminating soil from a surface |
GB2286603B (en) | 1994-02-14 | 1998-03-25 | Jeyes Group Plc | Bleach compositions |
US5578134A (en) | 1994-04-19 | 1996-11-26 | Ecolab Inc. | Method of sanitizing and destaining tableware |
US5571570A (en) | 1994-04-22 | 1996-11-05 | Red Spot Paint And Varnish Co., Inc. | UV curable blend compositions and processes |
DE4420127C1 (en) | 1994-06-09 | 1995-05-11 | Jun Heinz Stemmler | Means for improving carcass keeping quality |
US5585341A (en) * | 1995-02-27 | 1996-12-17 | Buckeye International, Inc. | Cleaner/degreaser concentrate compositions |
WO1996038522A1 (en) * | 1995-06-02 | 1996-12-05 | Ashland Inc. | Stable microemulsion cleaners having low volatile organic content |
ATE200514T1 (en) * | 1995-07-01 | 2001-04-15 | Bactria Gmbh & Co Kg Industrie | STORAGE-Stable COMPOSITION BASED ON PERACIDS |
CA2231459A1 (en) * | 1995-07-18 | 1997-02-06 | Unilever Plc | Concentrated aqueous degreasing cleanser |
FR2736936B1 (en) | 1995-07-19 | 1997-08-14 | Air Liquide | DEGREASING PROCESS BASED ON HYDROGEN PEROXIDE AND APPLICATIONS TO METAL ARTICLES |
GB9523222D0 (en) * | 1995-11-14 | 1996-01-17 | Reckitt & Colman Inc | Improved compositions containing organic compounds |
EP0881877B1 (en) | 1995-12-01 | 2005-01-26 | Minntech Corporation | Room temperature sterilant for medical devices |
EP0779357A1 (en) | 1995-12-16 | 1997-06-18 | The Procter & Gamble Company | Stable emulsions comprising a hydrophobic liquid ingredient |
US5827542A (en) * | 1996-02-12 | 1998-10-27 | Healthpoint, Ltd. | Quick acting chemical sterilant |
US5674828A (en) | 1996-04-08 | 1997-10-07 | Lever Brothers Company, Division Of Conopco, Inc. | Aqueous liquid compositions comprising peracid compounds and defined N-oxide compounds |
EP0805198A1 (en) | 1996-05-03 | 1997-11-05 | The Procter & Gamble Company | Cleaning compositions |
US5854187A (en) * | 1996-08-09 | 1998-12-29 | The Clorox Company | Microemulsion dilutable cleaner |
RU2102447C1 (en) | 1996-08-29 | 1998-01-20 | Борис Алексеевич Ильин | Detergent biocide agent |
AU723683B2 (en) | 1996-09-13 | 2000-08-31 | Minnesota Mining And Manufacturing Company | Floor finish compositions |
ATE238408T1 (en) | 1996-11-13 | 2003-05-15 | Procter & Gamble | AQUEOUS ALKALINE PEROXIDE BLEACHING COMPOSITIONS |
AU721046B2 (en) | 1996-12-19 | 2000-06-22 | Rohm And Haas Company | Coating substrates |
GB9626637D0 (en) * | 1996-12-21 | 1997-02-12 | Solvay Interox Ltd | Percarboxyilic acid solutions |
FR2761080B1 (en) * | 1997-03-21 | 2002-07-19 | Quadrimex | COMPOSITION BASED ON PERACIDS FOR THE CLEANING, DISINFECTION AND DECONTAMINATION OF SURFACES STAINED BY TOXIC AGENTS |
US5922665A (en) * | 1997-05-28 | 1999-07-13 | Minnesota Mining And Manufacturing Company | Aqueous cleaning composition including a nonionic surfactant and a very slightly water-soluble organic solvent suitable for hydrophobic soil removal |
US5968539A (en) | 1997-06-04 | 1999-10-19 | Procter & Gamble Company | Mild, rinse-off antimicrobial liquid cleansing compositions which provide residual benefit versus gram negative bacteria |
US5997893A (en) * | 1998-01-20 | 1999-12-07 | Ethicon, Inc. | Alcohol based anti-microbial compositions with cosmetic appearance |
US6015763A (en) * | 1998-02-17 | 2000-01-18 | Dotolo Research Corp. | Cleaner impregnated towel |
DE19811386A1 (en) * | 1998-03-16 | 1999-09-23 | Henkel Kgaa | Aqueous multiphase detergent forming temporary emulsion on shaking and used on hard surfaces |
DE19812591A1 (en) * | 1998-03-23 | 1999-09-30 | Degussa | Process for the control of plant pathogenic microorganisms in water circuits in greenhouses |
US6096266A (en) * | 1998-07-10 | 2000-08-01 | Box 03 International | Method for disinfecting and sterilizing microbial contaminated materials |
AU5005399A (en) * | 1998-07-22 | 2000-02-14 | Calgon Corporation | Synergistic antimicrobial composition of peroxyacetic acid and a phosphorus compound |
AU758625B2 (en) | 1998-08-20 | 2003-03-27 | Ecolab Inc. | The treatment of meat products |
US5977042A (en) | 1998-10-01 | 1999-11-02 | S. C. Johnson Commercial Markets, Inc. | Concentrated stripper composition and method |
EP1133321A1 (en) | 1998-11-23 | 2001-09-19 | Ecolab Incorporated | Non-corrosive sterilant composition |
IL128374A0 (en) * | 1999-02-04 | 2000-01-31 | Weitzman Abraham | Wide range cleaning and disinfecting preparations |
US5998358A (en) | 1999-03-23 | 1999-12-07 | Ecolab Inc. | Antimicrobial acid cleaner for use on organic or food soil |
US6444134B1 (en) | 2000-02-22 | 2002-09-03 | The Flecto Company, Inc. | Wood floor refinishing process and product |
US6593283B2 (en) * | 2000-04-28 | 2003-07-15 | Ecolab Inc. | Antimicrobial composition |
US7150884B1 (en) * | 2000-07-12 | 2006-12-19 | Ecolab Inc. | Composition for inhibition of microbial growth |
US6627593B2 (en) * | 2001-07-13 | 2003-09-30 | Ecolab Inc. | High concentration monoester peroxy dicarboxylic acid compositions, use solutions, and methods employing them |
US6962714B2 (en) | 2002-08-06 | 2005-11-08 | Ecolab, Inc. | Critical fluid antimicrobial compositions and their use and generation |
-
2001
- 2001-04-24 WO PCT/US2001/013118 patent/WO2001082694A1/en active IP Right Grant
- 2001-04-24 CA CA002407098A patent/CA2407098C/en not_active Expired - Lifetime
- 2001-04-24 AT AT01927310T patent/ATE306812T1/en not_active IP Right Cessation
- 2001-04-24 DE DE60114174T patent/DE60114174T2/en not_active Expired - Lifetime
- 2001-04-24 AU AU5377701A patent/AU5377701A/en active Pending
- 2001-04-24 AU AU2001253777A patent/AU2001253777B2/en not_active Expired
- 2001-04-24 MX MXPA02010640A patent/MXPA02010640A/en active IP Right Grant
- 2001-04-24 EP EP01927310A patent/EP1276372B1/en not_active Expired - Lifetime
- 2001-04-24 JP JP2001579587A patent/JP2004508291A/en not_active Withdrawn
-
2002
- 2002-02-28 US US10/086,765 patent/US6927237B2/en not_active Expired - Lifetime
-
2003
- 2003-04-14 US US10/413,635 patent/US6998369B2/en not_active Expired - Lifetime
-
2004
- 2004-12-02 US US11/002,349 patent/US20050096245A1/en not_active Abandoned
-
2005
- 2005-12-22 US US11/318,062 patent/US20060160712A1/en not_active Abandoned
-
2009
- 2009-02-09 US US12/368,070 patent/US8246906B2/en not_active Expired - Lifetime
Patent Citations (99)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2512640A (en) * | 1949-07-25 | 1950-06-27 | Buffalo Electro Chem Co | Treatment of raw plant tissue |
US3122417A (en) * | 1959-06-03 | 1964-02-25 | Henkel & Cie Gmbh | Stabilizing agent for peroxy-compounds and their solutions |
US3248281A (en) * | 1963-06-26 | 1966-04-26 | Dow Chemical Co | Iodine-peroxide-bisulfate antimicrobial composition |
US3514278A (en) * | 1968-01-08 | 1970-05-26 | Betz Laboratories | Slime control agent and methods of application |
US3895116A (en) * | 1971-11-29 | 1975-07-15 | Eastman Kodak Co | Mixtures of volatile fatty acids having anti-fungal and anti-bacterial activity |
US3867300A (en) * | 1972-08-10 | 1975-02-18 | Carbolabs Inc | Bactericidal composition |
US4330531A (en) * | 1976-03-26 | 1982-05-18 | Howard Alliger | Germ-killing materials |
US4191660A (en) * | 1978-02-27 | 1980-03-04 | International Flavors & Fragrances Inc. | Organoleptic uses of 1-(3,3-dimethyl-2-norbornyl)-2-propanone in cationic, anionic and nonionic detergents and soaps |
US4244884A (en) * | 1979-07-12 | 1981-01-13 | The Procter & Gamble Company | Continuous process for making peroxycarboxylic acids |
US4370199A (en) * | 1981-11-09 | 1983-01-25 | Westvaco Corporation | Enzymatic catalyzed biocide system |
US4501681A (en) * | 1981-12-23 | 1985-02-26 | Colgate-Palmolive Company | Detergent dish-washing composition |
US4529534A (en) * | 1982-08-19 | 1985-07-16 | The Procter & Gamble Company | Peroxyacid bleach compositions |
US4517330A (en) * | 1983-03-30 | 1985-05-14 | Rohm And Haas Company | Floor polish composition having improved durability |
US4655781A (en) * | 1984-07-02 | 1987-04-07 | The Clorox Company | Stable bleaching compositions |
US4592787A (en) * | 1984-11-05 | 1986-06-03 | The Dow Chemical Company | Composition useful for stripping photoresist polymers and method |
US4666622A (en) * | 1985-01-03 | 1987-05-19 | Lever Brothers Company | Stable thickened low pH liquid bleaching compositions containing inorganic peroxy compounds |
US4592488A (en) * | 1985-05-24 | 1986-06-03 | Simon Gilbert I | Method for the preparation of chemotherapeutic compositions for the treatment of periodontal disease, compositions therefor and use thereof |
US4923677A (en) * | 1985-08-07 | 1990-05-08 | Roy T. Witkin | Chemical sterilization |
US4997625A (en) * | 1985-08-07 | 1991-03-05 | Simon Gilbert I | Chemical sterilization |
US4738840A (en) * | 1986-03-03 | 1988-04-19 | Simon Gilbert I | Presurgical sterilization method |
US4900721A (en) * | 1986-06-09 | 1990-02-13 | Henkel Kommanditgesellschaft Auf Aktien | Disinfectants and their use for disinfecting the skin and mucous membrane |
US4802994A (en) * | 1986-07-17 | 1989-02-07 | Nalco Chemical Company | Biocide treatment to control sulfate-reducing bacteria in industrial process waste waters |
US5078896A (en) * | 1986-09-29 | 1992-01-07 | Akzo N.V. | Thickened aqueous cleaning compositions |
US4906617A (en) * | 1986-09-30 | 1990-03-06 | L'oreal | Pharmaceutical compositions containing saturated aromatic peroxides |
US4908306A (en) * | 1987-06-12 | 1990-03-13 | Life Technologies, Inc. | Human papillomavirus 56 nucleic acid hybridization probes and methods for employing the same |
US4945110A (en) * | 1987-06-15 | 1990-07-31 | Quali Tech, Inc. | Membrame-forming veterinary antibacterial teat dip |
US4891073A (en) * | 1987-07-13 | 1990-01-02 | Pennzoil Products Company | Method of treating surface with water-in-oil emulsion composition |
US4943414A (en) * | 1987-07-30 | 1990-07-24 | Johnson & Johnson Medical, Inc. | Method for vapor sterilizaton of articles having lumens |
US5015408A (en) * | 1988-03-19 | 1991-05-14 | Reckitt Gmbh | Denture cleaning tablet containing a bleach activator and an organic phosphonic acid stabilizer |
US5007969A (en) * | 1988-05-20 | 1991-04-16 | The Boeing Company | Low toxicity liquid solvent |
US4917815A (en) * | 1988-06-10 | 1990-04-17 | Sterling Drug Inc. | Stable aqueous aromatic percarboxylic acid solution |
US5292447A (en) * | 1988-06-14 | 1994-03-08 | Ausimont S.R.L. | Heterocyclic peroxides having n-amidic heteroatoms |
US5010109A (en) * | 1988-06-30 | 1991-04-23 | Chisso Corporation | Antimicrobial agent composition |
US5093140A (en) * | 1988-07-20 | 1992-03-03 | Eisai Co., Ltd. | Aqueous bactericide for animal treatment |
US4996062A (en) * | 1988-10-28 | 1991-02-26 | Stabra Ag | Glucose oxidase food treatment and storage method |
US5512309A (en) * | 1989-02-09 | 1996-04-30 | Rhone-Poulenc Inc. | Process for treating poultry carcasses to increase shelf-life |
US4937066A (en) * | 1989-06-22 | 1990-06-26 | David G. Vlock | Zinc containing oral compositions |
US5080831A (en) * | 1989-06-29 | 1992-01-14 | Buckeye International, Inc. | Aqueous cleaner/degreaser compositions |
US5591706A (en) * | 1989-08-08 | 1997-01-07 | Akzo Nobel N.V. | Aqueous peroxide compositions with improved safety profile |
US5298222A (en) * | 1989-08-09 | 1994-03-29 | Osteotech, Inc. | Process for disinfecting musculoskeletal tissue and tissues prepared thereby |
US5180749A (en) * | 1989-08-22 | 1993-01-19 | Sterling Winthrop, Inc. | Antimicrobial composition |
US5129824A (en) * | 1989-12-21 | 1992-07-14 | Keller Duane C | Method for treating periodontal disease |
US4997571A (en) * | 1990-01-05 | 1991-03-05 | Mogul Corporation | Method of treating water |
US4992108A (en) * | 1990-01-18 | 1991-02-12 | Ward Irl E | Photoresist stripping compositions |
US5004760A (en) * | 1990-02-16 | 1991-04-02 | The Dow Chemical Company | Biocidal foams |
US5080822A (en) * | 1990-04-10 | 1992-01-14 | Buckeye International, Inc. | Aqueous degreaser compositions containing an organic solvent and a solubilizing coupler |
US5122538A (en) * | 1990-07-23 | 1992-06-16 | Ecolab Inc. | Peroxy acid generator |
US5096501A (en) * | 1990-08-27 | 1992-03-17 | E. I. Du Pont De Nemours & Company | Environmentally safe cleaning process and cleaning composition useful therein |
US5635492A (en) * | 1990-08-31 | 1997-06-03 | Diversey Lever, Inc. | Teat treating compositions, production and use |
US5084239A (en) * | 1990-08-31 | 1992-01-28 | Abtox, Inc. | Plasma sterilizing process with pulsed antimicrobial agent treatment |
US5114718A (en) * | 1990-09-20 | 1992-05-19 | The Procter & Gamble Company | Sustained release compositions for treating periodontol disease |
US5597790A (en) * | 1990-10-22 | 1997-01-28 | The Procter & Gamble Company | Liquid detergent compositions containing a suspended peroxygen bleach |
US5391324A (en) * | 1991-02-01 | 1995-02-21 | Hoechst Aktiengesellschaft | Aqueous suspensions of peroxycarboxylic acids |
US5319018A (en) * | 1991-04-23 | 1994-06-07 | Rohm And Haas Company | Transition metal crosslinking of acid-containing polymers |
US5130124A (en) * | 1991-05-01 | 1992-07-14 | Isp Investments Inc. | Stabilized, aqueous, film-forming antimicrobial compositions of hydrogen peroxide |
US5773487A (en) * | 1991-05-15 | 1998-06-30 | Uv Coatings, Inc. | Finishing composition which is curable by UV light and method of using same |
US5419908A (en) * | 1991-06-04 | 1995-05-30 | Ecolab Inc. | Sanitizing composition comprising a blend of aromatic and polyunsaturated carboxylic acids |
US5508046A (en) * | 1991-07-15 | 1996-04-16 | Minntech Corporation | Stable, anticorrosive peracetic/peroxide sterilant |
US5200189A (en) * | 1991-07-23 | 1993-04-06 | Ecolab Inc. | Peroxyacid antimicrobial composition |
US5314687A (en) * | 1991-07-23 | 1994-05-24 | Ecolab Inc. | Peroxyacid antimicrobial composition |
US5489434A (en) * | 1991-07-23 | 1996-02-06 | Ecolab Inc. | Peroxyacid antimicrobail composition |
US5718910A (en) * | 1991-07-23 | 1998-02-17 | Ecolab Inc. | Peroxyacid antimicrobial composition |
US5494707A (en) * | 1991-09-12 | 1996-02-27 | Mannington Mills, Inc. | Resilient floor covering and method of making same |
US5208057A (en) * | 1991-11-12 | 1993-05-04 | Rohm And Haas Company | Process for butchering and disinfecting fowl |
US5176899A (en) * | 1991-11-25 | 1993-01-05 | Montgomery Robert E | Antimicrobial dentifrice |
US5230821A (en) * | 1991-12-03 | 1993-07-27 | E. I. Du Pont De Nemours And Company | Cleaning composition |
US5436868A (en) * | 1992-07-07 | 1995-07-25 | Oki Electric Industry Co., Ltd. | Word line selection circuit for selecting memory cells |
US5502148A (en) * | 1993-01-28 | 1996-03-26 | Bayer Aktiengesellschaft | Two-component polyurethane coating compositions and their use for coating or sealing floors |
US5409713A (en) * | 1993-03-17 | 1995-04-25 | Ecolab Inc. | Process for inhibition of microbial growth in aqueous transport streams |
US5637559A (en) * | 1993-03-30 | 1997-06-10 | Minnesota Mining And Manufacturing Company | Floor stripping composition and method |
US5744440A (en) * | 1993-03-30 | 1998-04-28 | Minnesota Mining And Manufacturing Company | Hard surface cleaning compositions including a very slightly water-soluble organic solvent |
US5529887A (en) * | 1993-03-31 | 1996-06-25 | Morton International, Inc. | Water soluble fluoride-containing solution for removing cured photoresist and solder resist mask |
US5616335A (en) * | 1993-05-05 | 1997-04-01 | Chemoxal S.A. | Stable thickened disinfecting aqueous composition containing an organic peroxy acid intended for human or animal use |
US5419848A (en) * | 1993-07-02 | 1995-05-30 | Buckeye International, Inc. | Aqueous degreaser emulsion compositions |
US5756139A (en) * | 1993-08-03 | 1998-05-26 | Solvay Interox Limited | Egg washing and disinfection process |
US5494588A (en) * | 1993-08-05 | 1996-02-27 | Nalco Chemical Company | Method and composition for inhibiting growth of microorganisms including peracetic acid and a second organic biocide |
US5785867A (en) * | 1993-08-05 | 1998-07-28 | Nalco Chemical Company | Method and composition for inhibiting growth of microorganisms including peracetic acid and a non-oxidizing biocide |
US5435808A (en) * | 1993-09-03 | 1995-07-25 | Birko Corporation | Hide raceway treatment and improved method of curing hides |
US5632676A (en) * | 1993-10-12 | 1997-05-27 | Fmc Corporation | Use of peracetic acid to sanitize processed fowl |
US6049002A (en) * | 1994-03-09 | 2000-04-11 | Kemira Chemicals B.V. | Method for the preparation of aqueous solutions containing performic acid as well as their use |
US5616616A (en) * | 1994-06-01 | 1997-04-01 | Minntech Corporation | Room Temperature sterilant |
US5902619A (en) * | 1994-12-02 | 1999-05-11 | Rubow; Ulrik | Method and apparatus for disinfecting or sterilizing foodstuffs and other articles |
US5595967A (en) * | 1995-02-03 | 1997-01-21 | The Procter & Gamble Company | Detergent compositions comprising multiperacid-forming bleach activators |
US5641530A (en) * | 1995-11-27 | 1997-06-24 | Eka Nobel Inc. | Method of disinfection |
US6010995A (en) * | 1995-12-28 | 2000-01-04 | Buckeye International, Inc. | No/low volatile organic compound cleaner/degreaser composition |
US5712239A (en) * | 1996-04-08 | 1998-01-27 | Lever Brothers Company, Division Of Conopco, Inc. | Aqueous liquid compositions comprising peracid compounds and substituted phenolic compounds |
US5900256A (en) * | 1996-09-18 | 1999-05-04 | Cottrell, Ltd. | Hydrogen peroxide disinfecting and sterilizing compositions |
US5891392A (en) * | 1996-11-12 | 1999-04-06 | Reckitt & Colman Inc. | Ready to use aqueous hard surface cleaning and disinfecting compositions containing hydrogen peroxide |
US6028104A (en) * | 1997-01-30 | 2000-02-22 | Ecolab Inc. | Use of peroxygen compounds in the control of hairy wart disease |
US6080416A (en) * | 1998-01-20 | 2000-06-27 | Ethicon, Inc. | Low tack lotion, gels and creams |
US6022551A (en) * | 1998-01-20 | 2000-02-08 | Ethicon, Inc. | Antimicrobial composition |
US6238685B1 (en) * | 1998-04-06 | 2001-05-29 | Ecolab Inc. | Peroxy acid treatment to control pathogenic organisms on growing plants |
US6033705A (en) * | 1998-07-08 | 2000-03-07 | Isaacs; Charles E. | Method for treating foodstuffs to reduce or prevent microbial activity |
US6010729A (en) * | 1998-08-20 | 2000-01-04 | Ecolab Inc. | Treatment of animal carcasses |
US6399689B1 (en) * | 1999-03-03 | 2002-06-04 | Lilly Industries, Inc. | Abrasion resistant coatings |
US6024986A (en) * | 1999-05-24 | 2000-02-15 | Ecolab Inc. | Method of protecting growing plants from the effects of plant pathogens |
US6506261B1 (en) * | 1999-06-24 | 2003-01-14 | Ecolab Inc. | Detergent compositions for the removal of complex organic or greasy soils |
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US6998369B2 (en) * | 2000-04-28 | 2006-02-14 | Ecolab Inc. | Antimicrobial composition |
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Also Published As
Publication number | Publication date |
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CA2407098A1 (en) | 2001-11-08 |
EP1276372B1 (en) | 2005-10-19 |
MXPA02010640A (en) | 2003-05-14 |
WO2001082694A1 (en) | 2001-11-08 |
US20090196939A1 (en) | 2009-08-06 |
DE60114174D1 (en) | 2006-03-02 |
CA2407098C (en) | 2009-12-29 |
US6998369B2 (en) | 2006-02-14 |
US6927237B2 (en) | 2005-08-09 |
DE60114174T2 (en) | 2006-07-20 |
US20050096245A1 (en) | 2005-05-05 |
AU5377701A (en) | 2001-11-12 |
US20020168422A1 (en) | 2002-11-14 |
ATE306812T1 (en) | 2005-11-15 |
US8246906B2 (en) | 2012-08-21 |
EP1276372A1 (en) | 2003-01-22 |
AU2001253777B2 (en) | 2005-05-19 |
US20030228996A1 (en) | 2003-12-11 |
JP2004508291A (en) | 2004-03-18 |
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