US20050124073A1 - Fat collection and preparation system and method - Google Patents
Fat collection and preparation system and method Download PDFInfo
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- US20050124073A1 US20050124073A1 US10/732,125 US73212503A US2005124073A1 US 20050124073 A1 US20050124073 A1 US 20050124073A1 US 73212503 A US73212503 A US 73212503A US 2005124073 A1 US2005124073 A1 US 2005124073A1
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- syringe
- centrifuge
- insert
- substances
- adapter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/32—Surgical cutting instruments
- A61B17/320016—Endoscopic cutting instruments, e.g. arthroscopes, resectoscopes
- A61B17/32002—Endoscopic cutting instruments, e.g. arthroscopes, resectoscopes with continuously rotating, oscillating or reciprocating cutting instruments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/80—Suction pumps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/80—Suction pumps
- A61M1/81—Piston pumps, e.g. syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/80—Suction pumps
- A61M1/81—Piston pumps, e.g. syringes
- A61M1/815—Piston pumps, e.g. syringes the barrel serving as aspiration container, e.g. in a breast pump
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/88—Draining devices having means for processing the drained fluid, e.g. an absorber
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B2017/00017—Electrical control of surgical instruments
- A61B2017/00199—Electrical control of surgical instruments with a console, e.g. a control panel with a display
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B2017/00973—Surgical instruments, devices or methods, e.g. tourniquets pedal-operated
- A61B2017/00977—Surgical instruments, devices or methods, e.g. tourniquets pedal-operated the depression depth determining the power rate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/08—Lipoids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Definitions
- the present invention relates to a fat collection and preparation system and method for harvesting fat from human tissue and preparing harvested fat for injection for cosmetic rejuvenation purposes, including relief of facial aging and wrinkling, as well as for other purposes.
- Fat transplantation involves: (a) harvesting fat tissue, along with other substances surrounding it from a harvesting site; (b) preparing the fat tissue; and then (c) injecting the fat tissue into the affected areas in the recipient.
- Fat tissue resides in the human body together with blood vessels, and other naturally occurring substances.
- a tumescent fluid is first injected into the harvesting site, which may be, e.g., another part of the recipient's body. The tumescent fluid swells or increases the volume of the tissue in the harvesting site, thus reducing blood loss during harvesting, and facilitating the removal of the tissue from the harvesting site.
- Tumescent fluid currently includes a mixture of salt solution, epinephrine and lidocaine, although other substances may be used in order to accomplish the same effect on the fat tissue.
- a mixture of fat tissue, tumescent fluid, and oil (created by the body as a result of harvesting trauma), is then harvested from the site under low vacuum pressure into harvesting syringes through specially designed cannulae inserted into the site.
- the segregated mixture then needs to be further manipulated in a variety of ways in order to remove the oil and tumescent fluid layers, isolate and clean the fat tissue or fatty layer, and transfer the fat tissue to smaller syringes, e.g., 1 cc syringes, for transplantation.
- the various manipulation techniques involve rotating the mixture and moving it from one container to another.
- the harvesting syringe containing the segregated mixture may be flipped around, first to place the tumescent fluid layer on top and decant the tumescent fluid from the mixture, and then to place the oil layer on top and decant the oil from the mixture.
- the fat tissue may then be injected into another container to be washed, and then injected into the smaller transplantation syringes, so that it may then be injected into the desired location.
- Each of these manipulations exposes at least some of the mixture and the fat tissue therein to air, in addition to bringing the mixture and tissue into contact with additional instruments and surfaces, and causing other trauma to the tissue.
- Each exposure to air reduces the viability of the fat tissue, and increases the risk of contamination.
- This invention may increase the desirability of using fat transplants for cosmetic rejuvenation by providing a more cost-effective solution for collecting and preparing fat, and improving the long-term results for fat transplantation.
- the long-term results of fat transplantation are improved by minimizing manipulation of the mixture and the fat tissue contained therein and maximizing the sterility of the mixture, the tissue, and the surrounding environment, at every point during the collection and preparation process, before the fat tissue or fat is injected into the desired location.
- This invention may include: a centrifuge; a centrifuge insert which is sterile and replaceable; a syringe with an asymmetrical head at its distal end, and a plunger with a removable plunger shaft and an aspiration port in the plunger head; and an assembly with an adapter having male luer connectors on each end to be coupled with luer ends or luer lock syringes, and a removable filter cap releasably coupleable with the adapter.
- a mixture of tumescent fluid, fat tissue and oil is first harvested with low vacuum pressure through a cannula into a harvesting syringe.
- the cannula and plunger shaft are removed from the syringe.
- the syringe is placed in an insertion cavity in the centrifuge insert.
- the centrifuge insert is placed on and coupled with the centrifuge. The centrifuge is manually actuated to gently separate or stratify the mixture in the syringe into layers or strata of oil, fat tissue and tumescent fluid with other denser substances.
- the tumescent fluid and other denser substances is decanted out of the distal end of the syringe through the replaceable filter cap coupled with the adapter, which is coupled with the distal or luer end of the syringe.
- the filter cap is removed by unscrewing it from the adapter, or if the filter is integrated into the adapter, by breaking off that portion of the adapter containing the filter, leaving an adapter attached to the syringe.
- the oil is removed from the top of the syringe through an aspiration port in the plunger head in the syringe.
- the plunger shaft is reattached to the plunger head in the harvesting syringe, and used to push the fat tissue through the adapter into smaller injection syringes, used to inject the fat tissue into the desired locations.
- a first syringe is mated with a first end of an adapter assembly.
- a filter assembly is engaged with a second end of the adapter assembly.
- the filter assembly include a filter attached to the rest of the filter assembly.
- the first syringe is inserted into a receiver in a centrifuge insert, such as an insert cavity.
- a combination of substances is releasably retained inside a chamber of the syringe.
- the combination includes oil, fat (e.g., fat tissue or cells), and a denser fluid, which may include tumescent fluid and/or other components denser than the fat or oil, e.g., blood, hormones, or other non-fat components which are harvested from the harvesting site along with the fat.
- the centrifuge insert is coupled with a rotatable centrifuge member of a centrifuge.
- the first syringe is centrifuged (along with other syringes which may be placed in the centrifuge insert) to stratify the combination into strata of the denser fluid, the fat and the oil.
- At least a portion of the denser fluid stratum is filtered from the first syringe through the filter. This filtering step may be accomplished, for example, either during or after the centrifuging process.
- the filter prevents passage of at least a portion of the fat stratum from the first syringe.
- At least a portion of the oil stratum is extracted from an opening in a plunger head in a proximal portion of the syringe. This extraction step may be accomplished, for example, during the centrifuging process, or after the centrifuging process.
- the filter assembly is disengaged or removed from the adapter assembly, e.g., by unscrewing or breaking off the filter assembly from the adapter assembly, once the denser fluid has been filtered from the first syringe.
- an assembly in an example embodiment, includes an adapter and a filter assembly.
- the adapter has a first section, a second section, a passageway from the first section to the second section, and a coupling.
- the first section of the adapter is mateable with a luer connector of a first syringe.
- the second section of the adapter is mateable with a luer connector of a second syringe.
- the filter assembly is releasably coupleable with the adapter via the coupling.
- the coupling may be, for example, a threaded engagement or a breakable connection between the adapter and the filter assembly.
- the filter assembly includes a filter configured to selectively allow passage of a first type of substance out of the first syringe and to prevent passage of a second type of substance out of the first syringe.
- a first syringe is mated with a first section of an adapter.
- a first type of substance is then removed from the first syringe through a filter connected to the adapter.
- the filter prevents passage of a second type of substance from the first syringe.
- a second syringe is then mated with a second section of the adapter. The second type of substance is then transferred from the first syringe to the second syringe through the adapter.
- a more dense substance of a combination of substances is removed from a first syringe through a filter attached to an adapter coupled with the first syringe.
- the filter is configured to permit passage of the more dense substance while preventing passage of a less dense substance from the first syringe.
- the less dense substance is transferred from the first syringe through the adapter.
- an assembly in an example embodiment, includes an adapter and a filter assembly.
- the adapter has a first end, a second end, a passageway between the first end and the second end, and a coupling.
- the first end of the adapter is configured to sealingly engage an opening in a first container, such as, e.g., a syringe.
- the second end of the adapter is configured to sealingly engage an opening in a second container, such as, e.g., another syringe.
- the coupling may be, for example, a threaded engagement or a breakable connection between the adapter and the filter assembly.
- the filter assembly is releasably coupleable with the adapter via the coupling.
- the filter assembly includes a filter configured to selectively allow passage of a first type of substance out of the first container, and to prevent passage of a second type of substance out of the first container.
- a syringe in an example embodiment, includes a chamber, a plunger shaft, and a plunger head.
- the chamber has an open proximal end and an opening in the distal end of the chamber. A center of the opening is offset from a central longitudinal axis of the chamber.
- the plunger head is releasably coupled with the plunger shaft. The plunger head and the plunger shaft are configured to be slidably and sealingly insertable through the open proximal end into the chamber to variate a volume of an interior of the chamber.
- a combination of substances in a chamber in a syringe are centrifuged, in order to separate the combination of substances in the chamber into strata according to densities of each of the substances in the combination.
- a plunger head is slidably and sealingly inserted into an open proximal end of the chamber.
- the chamber has an opening at the distal end of the chamber. The center of the opening is offset from the central longitudinal axis of the chamber.
- the plunger shaft is attached to the plunger head after the centrifuging step.
- an apparatus for centrifuging syringes includes an insert releasably coupleable with a rotatable centrifuge member of a centrifuge.
- the insert has at least one insert cavity configured to receive a syringe.
- an assembly for centrifuging syringes includes a centrifuge and an insert.
- the centrifuge includes a base, and a rotatable centrifuge member rotatably coupled with the base.
- the insert is releasably coupleable with the rotatable centrifuge member.
- the insert has at least one insert cavity configured to receive a syringe.
- a centrifuge insert is coupled with a rotatable centrifuge member of a centrifuge.
- the centrifuge insert has at least one insert cavity retaining at least one syringe within the insert.
- the syringe is configured to releasably retain a combination of substances.
- FIG. 1 illustrates a perspective view of an embodiment of a fat collection and preparation system according to the present invention.
- FIG. 2 illustrates a perspective view of a syringe containing a harvested mixture, and having a cannula attached at a distal end of the syringe, of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 3 illustrates a perspective view of a plunger of the syringe of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 4 illustrates a perspective view of an adapter and filter in an assembly of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 5 illustrates an exploded view of an embodiment of the adapter and filter in the assembly of FIG. 3 .
- FIG. 6 illustrates an exploded view of an embodiment of an adapter and filter in an embodiment of the fat collection and preparation system.
- FIG. 7 illustrates an exploded view of an embodiment of the adapter in the assembly, and the syringes of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 8 illustrates a perspective view of the insert and centrifuge of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 9 illustrates a perspective view of a hand-driven centrifuge of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 10 illustrates a cross-sectional view of the hand-driven centrifuge of FIG. 9 , along the line 10 - 10 of FIG. 9 .
- FIG. 11 illustrates a perspective view of an electrically-driven centrifuge of an embodiment of the fat collection and preparation system.
- FIG. 12 illustrates a cross-sectional view of the electrically-driven centrifuge of FIG. 11 , along the line 11 - 11 of FIG. 11 .
- FIG. 13 illustrates a top view of the rotatable centrifuge member of the centrifuge of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 14 illustrates a top view of the insert placed on the rotatable centrifuge member of FIG. 13 .
- FIG. 15 illustrates a cross-sectional view of the insert and the centrifuge of FIG. 9 .
- FIG. 16 illustrates a cross-sectional view of the syringes, assemblies, insert and centrifuge of FIG. 9 .
- FIG. 17 illustrates a partial perspective view of the insert, syringe and assembly of the embodiment of the fat collection and preparation system of FIG. 1 during centrifugation of the syringes.
- FIG. 18 illustrates a cross-sectional view of the insert, syringe and assembly of FIG. 17 , along the line 18 - 18 of FIG. 17 .
- FIG. 19 illustrates a cross-sectional view of the syringe and assembly of FIG. 5 during centrifugation.
- FIG. 20 illustrates a cross-sectional view of an insert, rotatable centrifuge member, shaft, and base of a centrifuge of an embodiment of the fat collection and preparation system.
- FIG. 21 illustrates a cross-sectional view of a rotatable centrifuge member, shaft, and base of a centrifuge of an embodiment of the fat collection and preparation system.
- FIG. 22 illustrates a top view of the rotatable centrifuge member of FIG. 21 .
- FIG. 23 illustrates a cross-sectional view of an insert, with the rotatable centrifuge member, shaft, and base of the centrifuge of FIG. 21 .
- FIG. 24 illustrates a top view of the insert and rotatable centrifuge member of FIG. 23 .
- FIG. 25 illustrates a cross-sectional view of an insert, with the rotatable centrifuge member, shaft, and base of the centrifuge of FIG. 21 .
- FIG. 26 illustrates a top view of the insert and rotatable centrifuge member of FIG. 25 .
- FIG. 27 illustrates a perspective view of the insert and a syringe of the embodiment of the fat collection and preparation system of FIG. 1 , which has been removed from the centrifuge and placed on a surface.
- FIG. 28 illustrates a perspective view of removal of oil after centrifugation of the embodiment of the fat collection and preparation system of FIG. 1 .
- FIG. 29 illustrates a perspective view of transferring fat from one syringe to another syringe through the adapter of the embodiment of the fat collection and preparation system of FIG. 1 .
- the system includes syringes 100 , an assembly 200 , a centrifuge insert 300 , and a centrifuge 500 .
- a mixture or combination of substances 400 including oil, fat, tumescent fluid and other denser fluid components, is harvested from a harvesting site, through a cannula 102 into a syringe 100 .
- the syringe 100 is placed or inserted (along with other syringes 100 ) in the centrifuge insert 300 .
- the syringes 100 are then subject to centrifugation within the centrifuge insert 300 , which is coupled with the centrifuge 500 .
- Oil 415 may be removed through an aspiration valve or port 161 accessing opening 168 in the plunger head 162 of the plunger 160 in the syringe 100 .
- Fat 425 may then be transferred into smaller syringes 600 , e.g., 1 cc syringes, for injection in facial wrinkles, depressions, or for other medical purposes.
- the extracted or removed oil may be discarded or used for other purposes.
- the syringe 100 used by an operator for harvesting the mixture or combination of substances 400 from a harvesting site may be designed as illustrated in FIGS. 2 to 4 , and FIGS. 18, 19 and 28 .
- the syringe 100 includes a chamber 120 having an opening 130 at its distal or luer end 112 , and an open proximal end 110 .
- the opening 130 at the luer end 112 may be positioned off-center or offset from a central longitudinal axis 114 of the syringe 100 , so that the opening 130 provides a distal-most outlet when the syringe 100 is positioned at an angle during centrifugation, as illustrated in FIGS. 18 and 19 .
- the offset or off-center position of the opening 130 allows for a larger portion of the denser fluid or substances 415 in the chamber 120 to be drained out of the syringe 100 during centrifugation.
- the offset or off-center position of opening 130 allows for the elimination of more of the denser substances 415 including the tumescent fluid from syringe 100 when syringe 100 is in an angled vertical position during centrifugation, without trapping any residual substances or fluid in the chamber 120 of syringe 100 .
- the syringe 100 also includes a plunger 160 slidably and sealingly insertable into the open proximal end 110 , as illustrated in FIGS. 2 and 3 , and FIGS. 28 and 29 .
- the movement of the plunger 160 variates an interior volume 122 of the chamber 120 .
- movement of the plunger 160 in the proximal direction 700 may be used to harvest the substances 400 into the chamber 120 , either alone, or with the use of an aspirator. Movement of the plunger 160 in the distal direction 702 decreases the interior volume 122 of the chamber 120 , and pushes contents in the chamber 120 out of the syringe 100 through the opening 130 .
- the plunger 160 includes a plunger head 162 and a plunger shaft 164 releasably attached or coupled with the plunger head 162 .
- the plunger shaft 164 may be threadably engaged with the plunger head 162 , and removed or unscrewed before the syringe 100 is subject to centrifugation, as illustrated in FIGS. 16 to 18 .
- the plunger head 162 may also include a removal device 166 for removing or extracting oil 415 or other substances in a proximal-most stratum 410 from the syringe 100 , either during or after centrifugation, as illustrated in FIGS. 2, 3 and 28 .
- the removal device 166 is configured such that, when not in use for removing or extracting oil 415 or other substances from syringe 100 , the removal device 166 substantially preserves the sealed enclosure of the interior volume 122 .
- the removal device 166 may include an aspiration valve or a port 161 , providing selective access to opening 168 .
- the opening 168 may be positioned off-center or to the side of the plunger shaft 164 , as illustrated in FIGS.
- the opening 168 may be positioned such that it is exposed only upon removal of the plunger shaft 164 . Regardless of the positioning of the opening 168 , the oil 415 or other substances in the proximal-most stratum 410 may be removed or extracted through the opening 168 after centrifugation of the syringe 100 and the substances 400 contained therein.
- the removal device 166 may also include a filter 169 covering the aspiration port or valve 161 , as illustrated in FIG. 3 .
- the filter 169 permits the oil in the proximal-most stratum 410 to travel in proximal direction 700 out of the syringe 100 , during centrifugation, while preventing passage of the fat 425 in the middle stratum 420 from the syringe 100 .
- a closure 167 may be provided to selectively close the opening 168 , preserving the enclosure of the chamber 120 while moving the plunger 160 in proximal direction 700 to draw substances 400 into the syringe 100 , and while moving the plunger 160 in distal direction 702 to push substances 400 out of the syringe 100 , as illustrated in FIGS. 2 and 3 .
- the luer end 112 of the syringe 100 is configured to accept, and be mated, coupled or otherwise engaged with adapter 230 in assembly 200 , as illustrated in FIGS. 4 to 7 , and FIG. 29 , or to mate or couple the syringe 100 with cannula 102 or other components, devices or assemblies, as illustrated in FIG. 2 .
- the luer end 112 may include a luer locking mechanism 142 , such as, for example, a female luer connector 144 configured to receive a male luer connector 244 from adapter 230 .
- the luer end 112 may have internal threads 146 to threadably engage external threads 254 on adapter 230 .
- Assembly 200 is configured to be secured, attached, engaged, connected, mated or otherwise coupled with syringe 100 , for use during the process of draining, filtering or otherwise removing denser substances 435 in the distal-most stratum 430 from syringe 100 , as illustrated in FIGS. 17 to 19 .
- Assembly 200 is also configured for use during the process of transferring fat cells or fat tissue 425 from fat stratum 420 into smaller syringes 600 , as illustrated in FIGS. 7 and 29 .
- assembly 200 includes adapter 230 and filter assembly 270 releasably coupleable with adapter 230 .
- Adapter 230 has a luer connector 240 at its proximal or first end 232 in its proximal or first section 233 , a luer connector 242 in its distal or second end 234 in its distal or second section 235 , a passageway 236 from proximal end 232 to distal end 234 , and a coupling 250 for releasably coupling filter assembly 270 with adapter 230 .
- Filtering assembly 270 includes filter cap 272 with filter 274 , as illustrated in FIGS. 4 to 6 .
- Filter 274 is integrated with filter cap 272 , and filter 274 is configured to permit or allow passage of denser substances 435 in the distal-most stratum 430 from syringe 100 , while preventing passage of the fat tissue or fat cells 425 in the middle stratum 420 from syringe 100 , when syringe 100 is coupled with adapter 230 and adapter 230 is coupled with filter assembly 270 , as illustrated in FIG. 19 .
- Filter 274 may cover opening 238 at distal end 234 of adapter 230 , or otherwise be positioned along passageway 236 with a surface area covering a cross section of passageway 236 , as illustrated in FIG. 5 .
- Filter cap 272 is configured to accept coupling 250 , and releasably engage, connect, couple or mate filter cap 272 to adapter 230 , as illustrated in FIGS. 5 and 6 .
- the filter cap 272 may be manufactured together with and attached to adapter 230 , with a coupling 250 , such as a breakable connector 252 , with a capability of being detached after use from the adapter 230 , as illustrated in FIG. 6 .
- coupling 250 may include external threads 254 on adapter 230 to be threadably engaged with internal threads 276 in the filter cap 272 , e.g., such as a 720-degree threading for strong and secure fit to syringe 100 , as illustrated in FIG. 5 .
- Filter cap 274 may be provided as a disposable component, capable of being used with any type of adapter 230 and any type of luer locking mechanism 144 on a syringe 100 .
- the adapter 230 is coupled with syringe 100 via the luer connector 240 , and adapter 230 is coupled with syringe 600 after removing the denser substances 435 from the distal-most stratum 430 via the luer connector 242 , as illustrated in FIGS. 7 and 29 .
- Luer connectors 240 and 242 of adapter 230 are releasably coupleable with proximal and distal sections 232 and 234 of adapter 230 with luer ends 112 and 612 of syringes 100 and 600 , respectively.
- a luer connector 240 on adapter 230 such as a male luer connector 244 is configured to couple adapter 230 with luer end 112 of syringe 100 , such as a female luer connector 144 of syringe 100 .
- a luer connector 242 such as a male luer connector 246 is configured to couple adapter 230 with luer end 612 of syringe 600 , such as a female luer connector 644 of syringe 600 .
- assembly 200 (including adapter 230 and filter assembly 270 coupled with adapter 230 ), is coupled with syringe 100 , for example, by snapping male luer connector 246 on adapter 230 with female luer connector 144 of syringe 100 , as illustrated in FIGS. 4 and 5 .
- the syringe 100 and assembly 200 are then inserted or placed in centrifuge insert 300 , as illustrated in FIGS. 1, 16 to 18 .
- Centrifuge insert 300 provides a sterile enclosure or environment for retaining syringes 100 during the centrifugation process in centrifuge 500 , as illustrated in FIGS. 1, 8 , and FIGS. 14 to 18 , and FIG. 27 .
- Centrifuge insert 300 may be molded of plastic, provided as a centrifuge insert mold, and may be disposable after a single use or removable from centrifuge 500 for sterilization before any set of syringes 100 are going to be centrifuged.
- Centrifuge insert 300 may be made or molded of a plastic material of sufficient rigidity to support syringes 100 after the insert 300 and syringes 100 have been removed from centrifuge 500 , in the same angled vertical position as when the syringes 100 and insert 300 are in the centrifuge 500 , as illustrated in FIG. 27 .
- Such a sufficiently rigid centrifuge insert 300 in addition to providing a sterile environment for centrifugation, functions as a syringe rack when the insert 300 together with syringes 100 is decoupled and removed from centrifuge 500 and placed or rested on a surface 800 .
- Centrifuge insert 300 has one or more insert cavities 310 configured to receive syringes 100 .
- Insert cavity 310 has an insert port or opening 312 at a proximal end 302 of insert cavity 310 wide enough to receive assembly 200 coupled with syringe 100 and syringe 100 .
- An outer surface 320 may be shaped (or has a shape configured) to provide a complementary fit to an inner surface 512 of a corresponding centrifuge cavity 510 in the rotatable centrifuge member 520 of centrifuge 500 (described further below), as illustrated in FIGS. 14 to 16 .
- the complementary fit of cavities 310 and 510 contributes to securely retaining the syringes 100 within their respective insert cavities 310 during centrifugation, and also contributes to coupling insert 300 with rotatable centrifuge member 520 .
- An inner surface 330 of insert cavity 310 is shaped to receive at least a portion of a syringe 100 and assembly 200 therein, before centrifugation, as illustrated in FIGS. 1, 8 and 14 to 18 , and 27 .
- the inner surface 330 of insert cavity 310 may also have a stop member 342 or stop surface 344 to prevent the luer end 112 of syringe 100 and assembly 200 from resting on a distal-most portion 332 of insert cavity 310 .
- the insert cavity 310 may have a distal segment 334 in the distal-most portion 332 with a smaller cross sectional area 336 than a proximal portion 316 of cavity 310 , preventing entry of the assembly 200 and luer end 112 of syringe 100 into the distal segment 334 .
- the distal-most portion 332 may receive the denser substances 435 from the distal-most stratum 430 in syringe 100 through filter 274 in assembly 200 during centrifugation of syringe 100 .
- a quantity of material 360 e.g., an absorbent powder, may be provided or arranged in the distal-most portion 332 to turn the received denser substances into a solid or gel-like substance upon contact adhering to an interior of portion 332 .
- absorbent material 360 keeps removed denser substances 435 away from the syringe 100 and prevents the removed denser substances 435 from spilling out of the centrifuge insert 300 , should the insert 300 be knocked over.
- the centrifuge insert 300 also has an insert lid or cover 370 releasably securable or attachable with the insert 300 , as illustrated in FIGS. 1, 8 , 15 and 27 .
- the insert cover 370 may be integrated with insert 300 , or attached to insert 300 with a connector 372 , and have a releasable catch 374 engageable with a locking member 319 on insert 300 to enclose the syringes 100 within insert 300 during centrifugation.
- the insert cover 370 may alternatively or also be threadably engageable with insert 300 . Once the cover 370 is closed, the syringes 100 are enclosed within insert 300 .
- This enclosure maintains the sterility of the environment surrounding the syringes 100 by not exposing them to an interior of centrifuge 500 during centrifugation, and preserves the sterility of the surrounding environment when the insert 300 is removed from centrifuge 500 .
- the enclosure also maintains the cleanliness of centrifuge 500 .
- the centrifuge 500 includes base 530 , a rotatable centrifuge member 520 rotatably coupled with base 530 , an actuating mechanism 540 coupled with the rotatable centrifuge member 520 to drive a rotation of rotatable centrifuge member 520 , and a centrifuge cover 550 .
- the centrifuge cover 550 may be releasably coupleable with the base 530 , thus surrounding the rotatable centrifuge member 520 , as illustrated in FIGS. 15 and 16 .
- the centrifuge cover 550 may alternatively be releasably coupleable with the rotatable centrifuge member 520 .
- the base 530 may be stationary and have a weighted perimeter 532 to increase the stability of the centrifuge 500 during centrifugation.
- the centrifuge 500 may be a hand-driven centrifuge 502 or an electrically operated centrifuge 504 (battery operated and/or with an A/C electric cord).
- the actuating mechanism 540 includes a manually actuated drive shaft 544 coupled with rotatable centrifuge member 520 .
- a button 542 coupled with a spring 541 and the drive shaft 544 may be depressed to actuate rotation of the drive shaft 544 , which, in turn, rotates the rotatable centrifuge member 520 , and the insert 300 with its syringes 100 and assemblies 200 , in order to centrifugate the syringes 100 and substances 400 contained therein.
- the button 542 may be located on the cover 550 , as illustrated, or on the base 530 .
- the button 542 may pumped or pushed three or four times over a three to four minute period to achieve sufficient rotational speed of the rotatable centrifuge member 520 and insert 300 .
- Hand-driven centrifuge 502 may be cost-effective, lightweight and easily transferable to and from an operating suite, together with its contents.
- the centrifuge 500 may be an electrically operated centrifuge 504 (battery powered and/or powered with an A/C electric cord), in which case, the actuating mechanism includes an electrically driven shaft 546 coupled with rotatable centrifuge member 520 .
- the rotation of the electrically driven shaft 546 is driven by an electric motor 545 which may be actuated or controlled with one or more buttons 548 .
- the button 548 may be located on the cover 550 , as illustrated, or on the base 530 .
- the rotatable centrifuge member 520 has at least one centrifuge cavity 510 , as mentioned above.
- the rotatable centrifuge member 520 has a plurality of centrifuge cavities 510 which are all substantially the same size, configured to provide a complementary fit for the plurality of insert cavities 310 in centrifuge insert 300 , the insert cavities 310 are configured to receive substantially the same sized syringes 100 (together with the assembly 200 on each syringe 100 ), as illustrated in FIGS. 10, 13 to 16 .
- each insert cavity 310 has a matching centrifuge cavity 510 .
- a rotatable centrifuge member 520 may alternatively be designed with differently sized centrifuge cavities 510 for accommodating differently sized insert cavities 210 and syringes 100 (together with the assembly 200 on each syringe 100 ), e.g., 10 cc syringes in one centrifugation and 20 cc syringes in another centrifugation, as illustrated in FIGS. 21 to 26 .
- a centrifuge insert 300 has insert cavities 310 dimensioned to receive at least a portion of particularly sized syringes 100 , e.g., 10 cc syringes, and their respective assemblies 200 .
- the insert cavities 310 have matching or corresponding centrifuge cavities 510 dimensioned for the insert cavities 310 and the 10 cc syringes 100 and assemblies 200 .
- the rotatable centrifuge member 520 also has larger centrifuge cavities 510 ′ dimensioned for accommodating larger insert cavities, e.g., for 20 cc syringes.
- Such larger centrifuge cavities 510 ′, and their open ends 511 ′ are covered by a proximal surface 315 of centrifuge insert 300 , as illustrated in FIGS. 23 and 24 .
- Another centrifuge insert 300 may be provided for another centrifugation with insert cavities 310 ′ which are configured to fit only the larger centrifuge cavities 510 ′ and leave the smaller centrifuge cavities 510 unmatched and covered by the proximal surface 315 of the insert 300 , as illustrated in FIGS. 25 and 26 .
- the complementary fit of the insert cavities 310 and centrifuge cavities 510 provides, as described above, a coupling to couple the insert 300 with the rotatable centrifuge member 520 , as illustrated in FIGS. 14 to 16 , and 23 to 26 .
- Insert 300 may have other additional mechanisms or components or members 318 to connect, engage, mate, secure or otherwise couple insert 300 with rotatable centrifuge member 520 , as illustrated in FIGS. 14 , and 23 to 26 .
- rotation of the rotatable centrifuge member 520 drives a rotation of the insert 300 .
- Insert 300 rotates with the rotatable member 520 during centrifugation.
- the rotatable centrifuge member 520 need not have any centrifuge cavities 510 , provided that rotatable centrifuge member 520 has another mechanism, component or member to couple the insert 300 with rotatable centrifuge member 520 , and the insert 300 is sufficiently rigid and securely maintains the syringes 100 in their respective insertion cavities 110 during centrifugation.
- various combinations of the embodiments of the syringe 100 , assembly 200 , centrifuge insert 300 , and centrifuge 500 may be used together with the methods described below to collect and prepare fat 425 for transplantation, without minimal manipulation of syringes 100 and the fat 425 .
- the combination or mixture of substances 400 including oil 415 , fat 425 (such as fat tissue or fat cells), and tumescent fluid and other denser substances 435 are harvested through a cannula 102 connected with syringe 100 , into chamber 120 of syringe 100 , as illustrated in FIG. 2 .
- the substances 400 may be harvested by aspirating the substance 400 from the harvesting site, and/or by pulling the plunger 160 in proximal direction 700 to draw the substances up through cannula 102 into chamber 120 of syringe 100 .
- the cannula 102 is then removed from syringe 100 .
- FIGS. 4 to 6 , and 19 An assembly 200 is then coupled with each such syringe 100 , as illustrated in FIGS. 4 to 6 , and 19 .
- a luer connector 240 of adapter 230 may engage a luer connector 142 of syringe 100 , coupling the adapter 230 with syringe 100 .
- the filter cap 272 is coupled with adapter 230 by threadably engaging the adapter 230 , or the filter cap 272 may be manufactured or provided already coupled with the adapter 230 in assembly 200 .
- the centrifuge insert 300 is coupled with the rotatable centrifuge member 520 , as illustrated in FIGS. 8, 14 , 15 and 20 .
- the insert cavities 310 are inserted into matching or corresponding centrifuge cavities 510 in rotatable centrifuge member 520 , and a small portion of absorbent material 360 is provided in the distal-most portion 332 of each insert cavity 310 , as illustrated in FIGS. 14 and 15 .
- the syringes 100 are placed or inserted, at least in partially, into insert cavities 310 in centrifuge insert 300 , as illustrated in FIGS. 1, 17 and 18 .
- the cover 370 of the centrifuge insert 300 is closed, to enclose the syringes 100 , their respective assemblies 200 and substances 400 within the insert 300 , as illustrated in FIG. 8 .
- the centrifuge 500 is then closed by placing cover 550 over the rotatable member 520 and secured or coupled with base 530 , as illustrated in FIGS. 9 and 16 .
- the syringes 100 , substances 400 and assemblies 200 are then subject to centrifugation or centrifuged in the centrifuge 500 , as illustrated in FIGS. 16 to 19 .
- Centrifugation may be accomplished by periodically depressing button 542 to actuate the spring 541 and rotate or otherwise spin the drive shaft 544 and the rotatable centrifuge member 520 for a hand-driven centrifuge 502 , or by adjusting button 548 to adjust the speed of the motor 545 rotating the drive shaft 546 and the rotatable centrifuge member 520 for an electrically operated centrifuge 504 , illustrated in FIGS. 9 to 12 .
- Rotatable centrifuge member 520 should be rotated at a sufficient rotational speed for a sufficient duration to: stratify the substances into separate oil stratum 410 , fat stratum 420 and denser substances stratum 430 (strata according to the densities of the substances 400 ), as illustrated in FIGS. 18 and 19 while minimizing the structural damage to the fat cells or tissue 425 in the fat stratum 420 during centrifugation.
- centrifuge 500 may be operated at a speed ranging from 500 to 1000 rpm, for approximately 2 to 4 minutes.
- the denser substances 435 in stratum 430 are decanted, drained, filtered, eliminated or otherwise removed from syringe 100 through filter 274 either during or after centrifugation.
- the denser substances 435 may be removed during centrifugation, provided that assemblies 200 including filter caps 272 are coupled with the syringes 100 before centrifugation ad the filter 274 is not covered during centrifugation, as illustrated in FIGS. 16 to 19 .
- the removed denser substances 435 may pass through filter 274 , into the distal-most portion 332 of insert cavity 310 .
- Rotation of syringe 100 provides a force acting radially outward 704 from a central vertical axis 514 of centrifuge 500 , leading the denser substances 435 to travel, forming the distal-most stratum 430 in the luer end 112 of syringe 100 , which is angled further away from axis 514 , and then further downward and outward through filter 274 .
- removed denser substances 435 may turn into a solid or gel-like substance 361 in distal-most portion 332 of insert cavity 310 .
- the removed denser substances 435 may remain in the insert 300 , as illustrated in FIG. 27 , and may be disposed with the insert 300 , after the removal from the insert 300 of syringes 100 with strata 410 and 420 of oil 415 and fat 425 , and assemblies 200 coupled with the syringes.
- the off-center or offset position of opening 130 on the luer end 112 of syringe 100 allows for the elimination of more of the denser substances 415 including the tumescent fluid from syringe 100 when syringe 100 is in an angled vertical position during centrifugation, without trapping any residual substances or fluid in the chamber 120 of syringe 100 , as illustrated in FIGS. 18 and 19 .
- the denser substances 435 in stratum 430 may also be removed from syringe 100 through filter 274 after centrifugation. In this case, the filter 274 is blocked with cover 275 during centrifugation, illustrated in FIGS. 5 and 6 . Once syringe 100 is stationary, cover 275 is removed, and denser substances 435 are permitted to drain or be filtered out through filter 274 .
- filter 274 does not permit passage of fat 425 from syringe 100 .
- the first fat 425 contacting or touching filter 274 creates an airtight seal protecting the rest of fat 425 from air contamination.
- Oil 415 is extracted or removed from the proximal stratum 410 in syringe 100 either during or after centrifugation, as illustrated in FIGS. 3 and 28 .
- Oil 415 may be removed during centrifugation if the plunger 160 includes a filter 169 covering opening 168 in plunger head 162 , as described above, and as illustrated in FIG. 3 .
- a closure 167 may be provided to selectively close the opening 168 and block off filter 169 during movement of plunger 160 in proximal direction 700 to draw substances 400 into syringe 100 , and during movement of plunger 160 in distal direction 702 to push substances 400 out of syringe 100 .
- the syringe or insert may either have a collector 380 or another mechanism, component, device or member for collecting such extracted or removed oil 415 , or the oil is extracted or removed after centrifugation in a more contained environment.
- a filter 169 is not necessary on port or valve 161 .
- the operator may first detach, disengage, decouple or otherwise remove insert 300 (with syringes 100 and their respective assemblies 200 and substances 400 contained therein) from rotatable centrifuge member 520 , place insert 300 on surface 800 , before opening insert cover 370 , in order to maintain cleanliness of centrifuge 500 , as illustrated in FIG. 27 .
- the operator opens insert cover 370 and may operate on syringe 100 while syringe 100 is retained in insert cavity 310 , as illustrated in FIG.
- the syringe 100 then contains only fat 425 .
- the operator may then use the same valve or port 161 and opening 168 to inject additional substances into syringe 100 , e.g., hormones or cleaning solutions which may be regarded as increasing the viability of the fat 425 .
- additional substances may be left mixed in with fat 425 , or centrifuged out again, in a second centrifuging process.
- the fat 425 is then transferred to a series of smaller syringes 600 , e.g., 1 cc syringes, for injection, as illustrated in FIG. 29 .
- the filter cap 274 illustrated in FIG. 5 or 6 is removed from assembly 200 by either breaking it off from adapter 230 or unscrewing it from adapter 230 , leaving syringe 100 coupled with proximal end 232 of adapter 230 .
- Syringe 600 is coupled with adapter 230 , as illustrated in FIG. 7 .
- Plunger shaft 164 is re-coupled or reattached with plunger head 162 , and movement of plunger 160 in distal direction 702 pushes fat 425 (and any additional substances, if any) out from syringe 100 , through passageway 236 in adapter 230 , and into syringe 600 , as illustrated in FIG. 29 .
- the fat 425 is thus collected and prepared for being injected into the desired location with syringe 600 .
Abstract
Description
- The present invention relates to a fat collection and preparation system and method for harvesting fat from human tissue and preparing harvested fat for injection for cosmetic rejuvenation purposes, including relief of facial aging and wrinkling, as well as for other purposes.
- In the field of plastic surgery, various substances and techniques are being developed in the area of cosmetic rejuvenation, for, inter alia, relieving facial aging and wrinkling. Current techniques involve the implantation of foreign substances into the affected areas of the recipient. Two foreign substances currently being used in this area are collagen and synthetic materials. Collagen implants involve the injection of the protein substance of the white fibers of (collagenous fibers) of connective tissue, such as skin, tendon, bone and cartilage. Current synthetic implants involve the injection of silicone, cadaver skin, and other synthesized materials. However, collagen and synthetic implants have various downsides, including infection, inflammation, rejection by the recipient, and limited durability of the injected materials. In an effort to eliminate these and other downsides associated with implanting collagen, synthetic or other foreign materials, fat transplantation (or transplantation of fat or fat tissue or cells) is being regarded as the future mainstay of cosmetic rejuvenation.
- Fat transplantation involves: (a) harvesting fat tissue, along with other substances surrounding it from a harvesting site; (b) preparing the fat tissue; and then (c) injecting the fat tissue into the affected areas in the recipient. Fat tissue resides in the human body together with blood vessels, and other naturally occurring substances. In order to harvest the fat tissue, a tumescent fluid is first injected into the harvesting site, which may be, e.g., another part of the recipient's body. The tumescent fluid swells or increases the volume of the tissue in the harvesting site, thus reducing blood loss during harvesting, and facilitating the removal of the tissue from the harvesting site. Tumescent fluid currently includes a mixture of salt solution, epinephrine and lidocaine, although other substances may be used in order to accomplish the same effect on the fat tissue. A mixture of fat tissue, tumescent fluid, and oil (created by the body as a result of harvesting trauma), is then harvested from the site under low vacuum pressure into harvesting syringes through specially designed cannulae inserted into the site.
- In order to prepare the fat tissue for injection, it must be separated from the other harvested substances in the mixture inside the syringe. Automated centrifuges have been used to segregate the mixture into the three layers of oil, fat tissue (or the fatty layer in the syringe), and tumescent fluid within each harvesting syringe. These automated centrifuges often rotate the harvesting syringes at a rate much higher than needed in order to segregate this type of mixture into its various layers, thus subjecting the tissue to unnecessary manipulation and trauma. Furthermore, since there is no easy, cost-effective way of maintaining the cleanliness of the automated centrifuges (and in particular, the surfaces surrounding the harvesting syringes), the sterility of the environment surrounding the fat tissue is often compromised.
- The segregated mixture then needs to be further manipulated in a variety of ways in order to remove the oil and tumescent fluid layers, isolate and clean the fat tissue or fatty layer, and transfer the fat tissue to smaller syringes, e.g., 1 cc syringes, for transplantation. The various manipulation techniques involve rotating the mixture and moving it from one container to another. For example, the harvesting syringe containing the segregated mixture may be flipped around, first to place the tumescent fluid layer on top and decant the tumescent fluid from the mixture, and then to place the oil layer on top and decant the oil from the mixture. The fat tissue may then be injected into another container to be washed, and then injected into the smaller transplantation syringes, so that it may then be injected into the desired location. Each of these manipulations exposes at least some of the mixture and the fat tissue therein to air, in addition to bringing the mixture and tissue into contact with additional instruments and surfaces, and causing other trauma to the tissue. Each exposure to air reduces the viability of the fat tissue, and increases the risk of contamination.
- Each manipulation of the mixture, and the fat tissue contained therein, affects the long term results of the transplantation, by comprising the sterility of the tissue and its surrounding environment, and subjecting the tissue to additional unnecessary trauma. The compromised sterility increases the chances of infection, inflammation and rejection of the fat transplant. The unnecessary trauma affects the viability and integrity of the fat tissue, and reduces the durability of the transplant. These effects, together with the high cost of the instruments involved in the process, decrease the desirability of using fat transplants for cosmetic rejuvenation.
- This invention may increase the desirability of using fat transplants for cosmetic rejuvenation by providing a more cost-effective solution for collecting and preparing fat, and improving the long-term results for fat transplantation. The long-term results of fat transplantation are improved by minimizing manipulation of the mixture and the fat tissue contained therein and maximizing the sterility of the mixture, the tissue, and the surrounding environment, at every point during the collection and preparation process, before the fat tissue or fat is injected into the desired location.
- This invention may include: a centrifuge; a centrifuge insert which is sterile and replaceable; a syringe with an asymmetrical head at its distal end, and a plunger with a removable plunger shaft and an aspiration port in the plunger head; and an assembly with an adapter having male luer connectors on each end to be coupled with luer ends or luer lock syringes, and a removable filter cap releasably coupleable with the adapter.
- In an example embodiment, a mixture of tumescent fluid, fat tissue and oil is first harvested with low vacuum pressure through a cannula into a harvesting syringe. The cannula and plunger shaft are removed from the syringe. The syringe is placed in an insertion cavity in the centrifuge insert. The centrifuge insert is placed on and coupled with the centrifuge. The centrifuge is manually actuated to gently separate or stratify the mixture in the syringe into layers or strata of oil, fat tissue and tumescent fluid with other denser substances. The tumescent fluid and other denser substances is decanted out of the distal end of the syringe through the replaceable filter cap coupled with the adapter, which is coupled with the distal or luer end of the syringe. The filter cap is removed by unscrewing it from the adapter, or if the filter is integrated into the adapter, by breaking off that portion of the adapter containing the filter, leaving an adapter attached to the syringe. The oil is removed from the top of the syringe through an aspiration port in the plunger head in the syringe. The plunger shaft is reattached to the plunger head in the harvesting syringe, and used to push the fat tissue through the adapter into smaller injection syringes, used to inject the fat tissue into the desired locations.
- In an example embodiment, a first syringe is mated with a first end of an adapter assembly. A filter assembly is engaged with a second end of the adapter assembly. The filter assembly include a filter attached to the rest of the filter assembly. The first syringe is inserted into a receiver in a centrifuge insert, such as an insert cavity. A combination of substances is releasably retained inside a chamber of the syringe. The combination includes oil, fat (e.g., fat tissue or cells), and a denser fluid, which may include tumescent fluid and/or other components denser than the fat or oil, e.g., blood, hormones, or other non-fat components which are harvested from the harvesting site along with the fat. The centrifuge insert is coupled with a rotatable centrifuge member of a centrifuge. The first syringe is centrifuged (along with other syringes which may be placed in the centrifuge insert) to stratify the combination into strata of the denser fluid, the fat and the oil. At least a portion of the denser fluid stratum is filtered from the first syringe through the filter. This filtering step may be accomplished, for example, either during or after the centrifuging process. The filter prevents passage of at least a portion of the fat stratum from the first syringe. Additionally, at least a portion of the oil stratum is extracted from an opening in a plunger head in a proximal portion of the syringe. This extraction step may be accomplished, for example, during the centrifuging process, or after the centrifuging process. The filter assembly is disengaged or removed from the adapter assembly, e.g., by unscrewing or breaking off the filter assembly from the adapter assembly, once the denser fluid has been filtered from the first syringe.
- In an example embodiment, an assembly includes an adapter and a filter assembly. The adapter has a first section, a second section, a passageway from the first section to the second section, and a coupling. The first section of the adapter is mateable with a luer connector of a first syringe. The second section of the adapter is mateable with a luer connector of a second syringe. The filter assembly is releasably coupleable with the adapter via the coupling. The coupling may be, for example, a threaded engagement or a breakable connection between the adapter and the filter assembly. The filter assembly includes a filter configured to selectively allow passage of a first type of substance out of the first syringe and to prevent passage of a second type of substance out of the first syringe.
- In an example embodiment, a first syringe is mated with a first section of an adapter. A first type of substance is then removed from the first syringe through a filter connected to the adapter. The filter prevents passage of a second type of substance from the first syringe. A second syringe is then mated with a second section of the adapter. The second type of substance is then transferred from the first syringe to the second syringe through the adapter.
- In an example embodiment, a more dense substance of a combination of substances is removed from a first syringe through a filter attached to an adapter coupled with the first syringe. The filter is configured to permit passage of the more dense substance while preventing passage of a less dense substance from the first syringe. The less dense substance is transferred from the first syringe through the adapter.
- In an example embodiment, an assembly includes an adapter and a filter assembly. The adapter has a first end, a second end, a passageway between the first end and the second end, and a coupling. The first end of the adapter is configured to sealingly engage an opening in a first container, such as, e.g., a syringe. The second end of the adapter is configured to sealingly engage an opening in a second container, such as, e.g., another syringe. The coupling may be, for example, a threaded engagement or a breakable connection between the adapter and the filter assembly. The filter assembly is releasably coupleable with the adapter via the coupling. The filter assembly includes a filter configured to selectively allow passage of a first type of substance out of the first container, and to prevent passage of a second type of substance out of the first container.
- In an example embodiment, a syringe includes a chamber, a plunger shaft, and a plunger head. The chamber has an open proximal end and an opening in the distal end of the chamber. A center of the opening is offset from a central longitudinal axis of the chamber. The plunger head is releasably coupled with the plunger shaft. The plunger head and the plunger shaft are configured to be slidably and sealingly insertable through the open proximal end into the chamber to variate a volume of an interior of the chamber.
- In an example embodiment, a combination of substances in a chamber in a syringe are centrifuged, in order to separate the combination of substances in the chamber into strata according to densities of each of the substances in the combination. A plunger head is slidably and sealingly inserted into an open proximal end of the chamber. The chamber has an opening at the distal end of the chamber. The center of the opening is offset from the central longitudinal axis of the chamber. The plunger shaft is attached to the plunger head after the centrifuging step.
- In an example embodiment, an apparatus for centrifuging syringes includes an insert releasably coupleable with a rotatable centrifuge member of a centrifuge. The insert has at least one insert cavity configured to receive a syringe.
- In an example embodiment, an assembly for centrifuging syringes includes a centrifuge and an insert. The centrifuge includes a base, and a rotatable centrifuge member rotatably coupled with the base. The insert is releasably coupleable with the rotatable centrifuge member. The insert has at least one insert cavity configured to receive a syringe.
- In an example embodiment, a centrifuge insert is coupled with a rotatable centrifuge member of a centrifuge. The centrifuge insert has at least one insert cavity retaining at least one syringe within the insert. The syringe is configured to releasably retain a combination of substances. After the coupling step, the rotatable centrifuge member, the centrifuge insert and the combination of substances is rotated.
-
FIG. 1 illustrates a perspective view of an embodiment of a fat collection and preparation system according to the present invention. -
FIG. 2 illustrates a perspective view of a syringe containing a harvested mixture, and having a cannula attached at a distal end of the syringe, of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 3 illustrates a perspective view of a plunger of the syringe of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 4 illustrates a perspective view of an adapter and filter in an assembly of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 5 illustrates an exploded view of an embodiment of the adapter and filter in the assembly ofFIG. 3 . -
FIG. 6 illustrates an exploded view of an embodiment of an adapter and filter in an embodiment of the fat collection and preparation system. -
FIG. 7 illustrates an exploded view of an embodiment of the adapter in the assembly, and the syringes of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 8 illustrates a perspective view of the insert and centrifuge of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 9 illustrates a perspective view of a hand-driven centrifuge of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 10 illustrates a cross-sectional view of the hand-driven centrifuge ofFIG. 9 , along the line 10-10 ofFIG. 9 . -
FIG. 11 illustrates a perspective view of an electrically-driven centrifuge of an embodiment of the fat collection and preparation system. -
FIG. 12 illustrates a cross-sectional view of the electrically-driven centrifuge ofFIG. 11 , along the line 11-11 ofFIG. 11 . -
FIG. 13 illustrates a top view of the rotatable centrifuge member of the centrifuge of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 14 illustrates a top view of the insert placed on the rotatable centrifuge member ofFIG. 13 . -
FIG. 15 illustrates a cross-sectional view of the insert and the centrifuge ofFIG. 9 . -
FIG. 16 illustrates a cross-sectional view of the syringes, assemblies, insert and centrifuge ofFIG. 9 . -
FIG. 17 illustrates a partial perspective view of the insert, syringe and assembly of the embodiment of the fat collection and preparation system ofFIG. 1 during centrifugation of the syringes. -
FIG. 18 illustrates a cross-sectional view of the insert, syringe and assembly ofFIG. 17 , along the line 18-18 ofFIG. 17 . -
FIG. 19 illustrates a cross-sectional view of the syringe and assembly ofFIG. 5 during centrifugation. -
FIG. 20 illustrates a cross-sectional view of an insert, rotatable centrifuge member, shaft, and base of a centrifuge of an embodiment of the fat collection and preparation system. -
FIG. 21 illustrates a cross-sectional view of a rotatable centrifuge member, shaft, and base of a centrifuge of an embodiment of the fat collection and preparation system. -
FIG. 22 illustrates a top view of the rotatable centrifuge member ofFIG. 21 . -
FIG. 23 illustrates a cross-sectional view of an insert, with the rotatable centrifuge member, shaft, and base of the centrifuge ofFIG. 21 . -
FIG. 24 illustrates a top view of the insert and rotatable centrifuge member ofFIG. 23 . -
FIG. 25 illustrates a cross-sectional view of an insert, with the rotatable centrifuge member, shaft, and base of the centrifuge ofFIG. 21 . -
FIG. 26 illustrates a top view of the insert and rotatable centrifuge member ofFIG. 25 . -
FIG. 27 illustrates a perspective view of the insert and a syringe of the embodiment of the fat collection and preparation system ofFIG. 1 , which has been removed from the centrifuge and placed on a surface. -
FIG. 28 illustrates a perspective view of removal of oil after centrifugation of the embodiment of the fat collection and preparation system ofFIG. 1 . -
FIG. 29 illustrates a perspective view of transferring fat from one syringe to another syringe through the adapter of the embodiment of the fat collection and preparation system ofFIG. 1 . - The present invention relates to a fat collection and preparation system and method for harvesting fat tissue and preparing harvested tissue for cosmetic rejuvenation and other purposes.
FIGS. 1 through 29 illustrate various aspects of the fat collection and preparation system and method according to the present invention. - As illustrated in
FIGS. 1 and 2 , the system includessyringes 100, anassembly 200, acentrifuge insert 300, and acentrifuge 500. A mixture or combination ofsubstances 400, including oil, fat, tumescent fluid and other denser fluid components, is harvested from a harvesting site, through acannula 102 into asyringe 100. Thesyringe 100 is placed or inserted (along with other syringes 100) in thecentrifuge insert 300. Thesyringes 100 are then subject to centrifugation within thecentrifuge insert 300, which is coupled with thecentrifuge 500. The centrifugation stratifies or separates the mixture or combination ofsubstances 400 into layers or strata according to their densities as illustrated inFIG. 6 , including a top orproximal-most stratum 410 ofoil 415, amiddle stratum 420 offat 425, including fat cells or fat tissue, and a bottom ordistal-most stratum 430 of denser debris orsubstances 435, including tumescent fluid, connective tissue, hormones and/or other non-fat denser components.Denser substances 435 is drained, filtered, decanted or otherwise removed out ofsyringe 100 either during or after centrifugation.Oil 415 may be removed through an aspiration valve orport 161 accessingopening 168 in theplunger head 162 of theplunger 160 in thesyringe 100.Fat 425 may then be transferred intosmaller syringes 600, e.g., 1 cc syringes, for injection in facial wrinkles, depressions, or for other medical purposes. The extracted or removed oil may be discarded or used for other purposes. - The
syringe 100 used by an operator for harvesting the mixture or combination ofsubstances 400 from a harvesting site may be designed as illustrated in FIGS. 2 to 4, andFIGS. 18, 19 and 28. Thesyringe 100 includes achamber 120 having anopening 130 at its distal orluer end 112, and an openproximal end 110. Theopening 130 at theluer end 112 may be positioned off-center or offset from a centrallongitudinal axis 114 of thesyringe 100, so that theopening 130 provides a distal-most outlet when thesyringe 100 is positioned at an angle during centrifugation, as illustrated inFIGS. 18 and 19 . The offset or off-center position of theopening 130 allows for a larger portion of the denser fluid orsubstances 415 in thechamber 120 to be drained out of thesyringe 100 during centrifugation. In other words, the offset or off-center position ofopening 130, allows for the elimination of more of thedenser substances 415 including the tumescent fluid fromsyringe 100 whensyringe 100 is in an angled vertical position during centrifugation, without trapping any residual substances or fluid in thechamber 120 ofsyringe 100. - The
syringe 100 also includes aplunger 160 slidably and sealingly insertable into the openproximal end 110, as illustrated inFIGS. 2 and 3 , andFIGS. 28 and 29 . The movement of theplunger 160 variates aninterior volume 122 of thechamber 120. For example, movement of theplunger 160 in theproximal direction 700, may be used to harvest thesubstances 400 into thechamber 120, either alone, or with the use of an aspirator. Movement of theplunger 160 in thedistal direction 702 decreases theinterior volume 122 of thechamber 120, and pushes contents in thechamber 120 out of thesyringe 100 through theopening 130. Theplunger 160 includes aplunger head 162 and aplunger shaft 164 releasably attached or coupled with theplunger head 162. Theplunger shaft 164 may be threadably engaged with theplunger head 162, and removed or unscrewed before thesyringe 100 is subject to centrifugation, as illustrated in FIGS. 16 to 18. - The
plunger head 162 may also include aremoval device 166 for removing or extractingoil 415 or other substances in aproximal-most stratum 410 from thesyringe 100, either during or after centrifugation, as illustrated inFIGS. 2, 3 and 28. Theremoval device 166 is configured such that, when not in use for removing or extractingoil 415 or other substances fromsyringe 100, theremoval device 166 substantially preserves the sealed enclosure of theinterior volume 122. Theremoval device 166 may include an aspiration valve or aport 161, providing selective access toopening 168. Theopening 168 may be positioned off-center or to the side of theplunger shaft 164, as illustrated inFIGS. 2, 3 and 28, or theopening 168 may be positioned such that it is exposed only upon removal of theplunger shaft 164. Regardless of the positioning of theopening 168, theoil 415 or other substances in theproximal-most stratum 410 may be removed or extracted through theopening 168 after centrifugation of thesyringe 100 and thesubstances 400 contained therein. - Alternatively, the
removal device 166 may also include afilter 169 covering the aspiration port orvalve 161, as illustrated inFIG. 3 . Thefilter 169 permits the oil in theproximal-most stratum 410 to travel inproximal direction 700 out of thesyringe 100, during centrifugation, while preventing passage of the fat 425 in themiddle stratum 420 from thesyringe 100. Aclosure 167 may be provided to selectively close theopening 168, preserving the enclosure of thechamber 120 while moving theplunger 160 inproximal direction 700 to drawsubstances 400 into thesyringe 100, and while moving theplunger 160 indistal direction 702 to pushsubstances 400 out of thesyringe 100, as illustrated inFIGS. 2 and 3 . - The
luer end 112 of thesyringe 100 is configured to accept, and be mated, coupled or otherwise engaged withadapter 230 inassembly 200, as illustrated in FIGS. 4 to 7, andFIG. 29 , or to mate or couple thesyringe 100 withcannula 102 or other components, devices or assemblies, as illustrated inFIG. 2 . Theluer end 112 may include aluer locking mechanism 142, such as, for example, afemale luer connector 144 configured to receive amale luer connector 244 fromadapter 230. Theluer end 112 may haveinternal threads 146 to threadably engageexternal threads 254 onadapter 230. -
Assembly 200 is configured to be secured, attached, engaged, connected, mated or otherwise coupled withsyringe 100, for use during the process of draining, filtering or otherwise removingdenser substances 435 in thedistal-most stratum 430 fromsyringe 100, as illustrated in FIGS. 17 to 19.Assembly 200 is also configured for use during the process of transferring fat cells orfat tissue 425 fromfat stratum 420 intosmaller syringes 600, as illustrated inFIGS. 7 and 29 . As illustrated in FIGS. 4 to 7, andFIG. 19 ,assembly 200 includesadapter 230 andfilter assembly 270 releasably coupleable withadapter 230.Adapter 230 has aluer connector 240 at its proximal orfirst end 232 in its proximal orfirst section 233, aluer connector 242 in its distal orsecond end 234 in its distal orsecond section 235, apassageway 236 fromproximal end 232 todistal end 234, and acoupling 250 for releasablycoupling filter assembly 270 withadapter 230. -
Filtering assembly 270 includesfilter cap 272 withfilter 274, as illustrated in FIGS. 4 to 6.Filter 274 is integrated withfilter cap 272, and filter 274 is configured to permit or allow passage ofdenser substances 435 in thedistal-most stratum 430 fromsyringe 100, while preventing passage of the fat tissue orfat cells 425 in themiddle stratum 420 fromsyringe 100, whensyringe 100 is coupled withadapter 230 andadapter 230 is coupled withfilter assembly 270, as illustrated inFIG. 19 . Once denser substances including tumescent fluid is decanted, filtered, drained or otherwise removed throughfilter 274, thefirst fat 425 from themiddle stratum 420 touchingfilter 274 will create an airtight seal protecting the rest of the fat from air contamination. As illustrated inFIGS. 16 through 19 , the denser substances may be removed throughfilter 274 during centrifugation ofsyringe 100. Alternatively, acover 275 may be placed overfilter 274, as illustrated inFIGS. 5 and 6 before centrifugation and removed after centrifugation, to permit thedenser substances 435 indistal-most stratum 430 to be drained or decanted out throughfilter 274, oncesyringe 100 is stationary. -
Filter 274 may cover opening 238 atdistal end 234 ofadapter 230, or otherwise be positioned alongpassageway 236 with a surface area covering a cross section ofpassageway 236, as illustrated inFIG. 5 .Filter cap 272 is configured to acceptcoupling 250, and releasably engage, connect, couple ormate filter cap 272 toadapter 230, as illustrated inFIGS. 5 and 6 . Thefilter cap 272 may be manufactured together with and attached toadapter 230, with acoupling 250, such as abreakable connector 252, with a capability of being detached after use from theadapter 230, as illustrated inFIG. 6 . Alternatively,coupling 250 may includeexternal threads 254 onadapter 230 to be threadably engaged withinternal threads 276 in thefilter cap 272, e.g., such as a 720-degree threading for strong and secure fit tosyringe 100, as illustrated inFIG. 5 .Filter cap 274 may be provided as a disposable component, capable of being used with any type ofadapter 230 and any type ofluer locking mechanism 144 on asyringe 100. - The
adapter 230 is coupled withsyringe 100 via theluer connector 240, andadapter 230 is coupled withsyringe 600 after removing thedenser substances 435 from thedistal-most stratum 430 via theluer connector 242, as illustrated inFIGS. 7 and 29 .Luer connectors adapter 230 are releasably coupleable with proximal anddistal sections adapter 230 with luer ends 112 and 612 ofsyringes luer connector 240 onadapter 230 such as amale luer connector 244 is configured to coupleadapter 230 withluer end 112 ofsyringe 100, such as afemale luer connector 144 ofsyringe 100. Aluer connector 242 such as amale luer connector 246 is configured to coupleadapter 230 withluer end 612 ofsyringe 600, such as afemale luer connector 644 ofsyringe 600. Whenadapter 230 is coupled withsyringes syringe 100 may be transferred throughpassageway 236 intosyringe 600. - After
substances 400 have been harvested from the harvesting site intosyringe 100, before centrifugation, and transferring fat fromsyringe 100 tosyringe 600, assembly 200 (includingadapter 230 andfilter assembly 270 coupled with adapter 230), is coupled withsyringe 100, for example, by snappingmale luer connector 246 onadapter 230 withfemale luer connector 144 ofsyringe 100, as illustrated inFIGS. 4 and 5 . Thesyringe 100 andassembly 200 are then inserted or placed incentrifuge insert 300, as illustrated inFIGS. 1, 16 to 18. -
Centrifuge insert 300 provides a sterile enclosure or environment for retainingsyringes 100 during the centrifugation process incentrifuge 500, as illustrated inFIGS. 1, 8 , and FIGS. 14 to 18, andFIG. 27 .Centrifuge insert 300 may be molded of plastic, provided as a centrifuge insert mold, and may be disposable after a single use or removable fromcentrifuge 500 for sterilization before any set ofsyringes 100 are going to be centrifuged.Centrifuge insert 300 may be made or molded of a plastic material of sufficient rigidity to supportsyringes 100 after theinsert 300 andsyringes 100 have been removed fromcentrifuge 500, in the same angled vertical position as when thesyringes 100 and insert 300 are in thecentrifuge 500, as illustrated inFIG. 27 . Such a sufficientlyrigid centrifuge insert 300, in addition to providing a sterile environment for centrifugation, functions as a syringe rack when theinsert 300 together withsyringes 100 is decoupled and removed fromcentrifuge 500 and placed or rested on asurface 800. -
Centrifuge insert 300 has one ormore insert cavities 310 configured to receivesyringes 100.Insert cavity 310 has an insert port or opening 312 at aproximal end 302 ofinsert cavity 310 wide enough to receiveassembly 200 coupled withsyringe 100 andsyringe 100. Anouter surface 320 may be shaped (or has a shape configured) to provide a complementary fit to aninner surface 512 of acorresponding centrifuge cavity 510 in therotatable centrifuge member 520 of centrifuge 500 (described further below), as illustrated in FIGS. 14 to 16. The complementary fit ofcavities syringes 100 within theirrespective insert cavities 310 during centrifugation, and also contributes to coupling insert 300 withrotatable centrifuge member 520. - An
inner surface 330 ofinsert cavity 310 is shaped to receive at least a portion of asyringe 100 andassembly 200 therein, before centrifugation, as illustrated inFIGS. 1, 8 and 14 to 18, and 27. Theinner surface 330 ofinsert cavity 310 may also have astop member 342 or stopsurface 344 to prevent theluer end 112 ofsyringe 100 and assembly 200 from resting on adistal-most portion 332 ofinsert cavity 310. Theinsert cavity 310 may have adistal segment 334 in thedistal-most portion 332 with a smaller crosssectional area 336 than aproximal portion 316 ofcavity 310, preventing entry of theassembly 200 andluer end 112 ofsyringe 100 into thedistal segment 334. Thedistal-most portion 332 may receive thedenser substances 435 from thedistal-most stratum 430 insyringe 100 throughfilter 274 inassembly 200 during centrifugation ofsyringe 100. A quantity ofmaterial 360, e.g., an absorbent powder, may be provided or arranged in thedistal-most portion 332 to turn the received denser substances into a solid or gel-like substance upon contact adhering to an interior ofportion 332. The presence ofabsorbent material 360 keeps removeddenser substances 435 away from thesyringe 100 and prevents the removeddenser substances 435 from spilling out of thecentrifuge insert 300, should the insert 300 be knocked over. - The
centrifuge insert 300 also has an insert lid or cover 370 releasably securable or attachable with theinsert 300, as illustrated inFIGS. 1, 8 , 15 and 27. Theinsert cover 370 may be integrated withinsert 300, or attached to insert 300 with aconnector 372, and have areleasable catch 374 engageable with a lockingmember 319 oninsert 300 to enclose thesyringes 100 withininsert 300 during centrifugation. Theinsert cover 370 may alternatively or also be threadably engageable withinsert 300. Once thecover 370 is closed, thesyringes 100 are enclosed withininsert 300. This enclosure maintains the sterility of the environment surrounding thesyringes 100 by not exposing them to an interior ofcentrifuge 500 during centrifugation, and preserves the sterility of the surrounding environment when theinsert 300 is removed fromcentrifuge 500. The enclosure also maintains the cleanliness ofcentrifuge 500. - As illustrated in
FIGS. 1, 8 to 16, thecentrifuge 500 includesbase 530, arotatable centrifuge member 520 rotatably coupled withbase 530, anactuating mechanism 540 coupled with therotatable centrifuge member 520 to drive a rotation ofrotatable centrifuge member 520, and acentrifuge cover 550. Thecentrifuge cover 550 may be releasably coupleable with thebase 530, thus surrounding therotatable centrifuge member 520, as illustrated inFIGS. 15 and 16 . Thecentrifuge cover 550 may alternatively be releasably coupleable with therotatable centrifuge member 520. The base 530 may be stationary and have aweighted perimeter 532 to increase the stability of thecentrifuge 500 during centrifugation. Thecentrifuge 500 may be a hand-drivencentrifuge 502 or an electrically operated centrifuge 504 (battery operated and/or with an A/C electric cord). - In the case of a hand-driven
centrifuge 502, as illustrated inFIGS. 9 and 10 , theactuating mechanism 540 includes a manually actuateddrive shaft 544 coupled withrotatable centrifuge member 520. Abutton 542 coupled with aspring 541 and thedrive shaft 544 may be depressed to actuate rotation of thedrive shaft 544, which, in turn, rotates therotatable centrifuge member 520, and theinsert 300 with itssyringes 100 andassemblies 200, in order to centrifugate thesyringes 100 andsubstances 400 contained therein. Thebutton 542 may be located on thecover 550, as illustrated, or on thebase 530. Thebutton 542 may pumped or pushed three or four times over a three to four minute period to achieve sufficient rotational speed of therotatable centrifuge member 520 and insert 300. Hand-drivencentrifuge 502 may be cost-effective, lightweight and easily transferable to and from an operating suite, together with its contents. - Alternatively, as illustrated in
FIGS. 11 and 12 , thecentrifuge 500 may be an electrically operated centrifuge 504 (battery powered and/or powered with an A/C electric cord), in which case, the actuating mechanism includes an electrically drivenshaft 546 coupled withrotatable centrifuge member 520. The rotation of the electrically drivenshaft 546 is driven by anelectric motor 545 which may be actuated or controlled with one ormore buttons 548. Thebutton 548 may be located on thecover 550, as illustrated, or on thebase 530. - The
rotatable centrifuge member 520 has at least onecentrifuge cavity 510, as mentioned above. In one embodiment, therotatable centrifuge member 520 has a plurality ofcentrifuge cavities 510 which are all substantially the same size, configured to provide a complementary fit for the plurality ofinsert cavities 310 incentrifuge insert 300, theinsert cavities 310 are configured to receive substantially the same sized syringes 100 (together with theassembly 200 on each syringe 100), as illustrated inFIGS. 10, 13 to 16. In such an embodiment, eachinsert cavity 310 has a matchingcentrifuge cavity 510. - In an embodiment, a
rotatable centrifuge member 520 may alternatively be designed with differentlysized centrifuge cavities 510 for accommodating differently sized insert cavities 210 and syringes 100 (together with theassembly 200 on each syringe 100), e.g., 10 cc syringes in one centrifugation and 20 cc syringes in another centrifugation, as illustrated in FIGS. 21 to 26. In this other embodiment, acentrifuge insert 300 hasinsert cavities 310 dimensioned to receive at least a portion of particularlysized syringes 100, e.g., 10 cc syringes, and theirrespective assemblies 200. The insert cavities 310 have matching orcorresponding centrifuge cavities 510 dimensioned for theinsert cavities 310 and the 10cc syringes 100 andassemblies 200. Therotatable centrifuge member 520 also haslarger centrifuge cavities 510′ dimensioned for accommodating larger insert cavities, e.g., for 20 cc syringes. Suchlarger centrifuge cavities 510′, and theiropen ends 511′ are covered by aproximal surface 315 ofcentrifuge insert 300, as illustrated inFIGS. 23 and 24 . Anothercentrifuge insert 300 may be provided for another centrifugation withinsert cavities 310′ which are configured to fit only thelarger centrifuge cavities 510′ and leave thesmaller centrifuge cavities 510 unmatched and covered by theproximal surface 315 of theinsert 300, as illustrated inFIGS. 25 and 26 . - In either embodiment, the complementary fit of the
insert cavities 310 andcentrifuge cavities 510 provides, as described above, a coupling to couple theinsert 300 with therotatable centrifuge member 520, as illustrated in FIGS. 14 to 16, and 23 to 26.Insert 300 may have other additional mechanisms or components ormembers 318 to connect, engage, mate, secure or otherwise couple insert 300 withrotatable centrifuge member 520, as illustrated inFIGS. 14 , and 23 to 26. Onceinsert 300 is coupled withrotatable centrifuge member 520, rotation of therotatable centrifuge member 520 drives a rotation of theinsert 300.Insert 300 rotates with therotatable member 520 during centrifugation. - In an embodiment illustrated in
FIG. 20 , therotatable centrifuge member 520 need not have anycentrifuge cavities 510, provided thatrotatable centrifuge member 520 has another mechanism, component or member to couple theinsert 300 withrotatable centrifuge member 520, and theinsert 300 is sufficiently rigid and securely maintains thesyringes 100 in theirrespective insertion cavities 110 during centrifugation. - In operation, various combinations of the embodiments of the
syringe 100,assembly 200,centrifuge insert 300, andcentrifuge 500 may be used together with the methods described below to collect and prepare fat 425 for transplantation, without minimal manipulation ofsyringes 100 and the fat 425. - After a fat harvesting site is injected with tumescent fluid, the combination or mixture of
substances 400, includingoil 415, fat 425 (such as fat tissue or fat cells), and tumescent fluid and otherdenser substances 435 are harvested through acannula 102 connected withsyringe 100, intochamber 120 ofsyringe 100, as illustrated inFIG. 2 . Thesubstances 400 may be harvested by aspirating thesubstance 400 from the harvesting site, and/or by pulling theplunger 160 inproximal direction 700 to draw the substances up throughcannula 102 intochamber 120 ofsyringe 100. Thecannula 102 is then removed fromsyringe 100. - An
assembly 200 is then coupled with eachsuch syringe 100, as illustrated in FIGS. 4 to 6, and 19. Specifically, aluer connector 240 ofadapter 230 may engage aluer connector 142 ofsyringe 100, coupling theadapter 230 withsyringe 100. Thefilter cap 272 is coupled withadapter 230 by threadably engaging theadapter 230, or thefilter cap 272 may be manufactured or provided already coupled with theadapter 230 inassembly 200. - The
centrifuge insert 300 is coupled with therotatable centrifuge member 520, as illustrated inFIGS. 8, 14 , 15 and 20. The insert cavities 310 are inserted into matching orcorresponding centrifuge cavities 510 inrotatable centrifuge member 520, and a small portion ofabsorbent material 360 is provided in thedistal-most portion 332 of eachinsert cavity 310, as illustrated inFIGS. 14 and 15 . - The
syringes 100, together with theirrespective assemblies 200, are placed or inserted, at least in partially, intoinsert cavities 310 incentrifuge insert 300, as illustrated inFIGS. 1, 17 and 18. Thecover 370 of thecentrifuge insert 300 is closed, to enclose thesyringes 100, theirrespective assemblies 200 andsubstances 400 within theinsert 300, as illustrated inFIG. 8 . Thecentrifuge 500 is then closed by placingcover 550 over therotatable member 520 and secured or coupled withbase 530, as illustrated inFIGS. 9 and 16 . - The
syringes 100,substances 400 andassemblies 200 are then subject to centrifugation or centrifuged in thecentrifuge 500, as illustrated in FIGS. 16 to 19. Centrifugation may be accomplished by periodicallydepressing button 542 to actuate thespring 541 and rotate or otherwise spin thedrive shaft 544 and therotatable centrifuge member 520 for a hand-drivencentrifuge 502, or by adjustingbutton 548 to adjust the speed of themotor 545 rotating thedrive shaft 546 and therotatable centrifuge member 520 for an electrically operatedcentrifuge 504, illustrated in FIGS. 9 to 12.Rotatable centrifuge member 520 should be rotated at a sufficient rotational speed for a sufficient duration to: stratify the substances intoseparate oil stratum 410,fat stratum 420 and denser substances stratum 430 (strata according to the densities of the substances 400), as illustrated inFIGS. 18 and 19 while minimizing the structural damage to the fat cells ortissue 425 in thefat stratum 420 during centrifugation. For example,centrifuge 500 may be operated at a speed ranging from 500 to 1000 rpm, for approximately 2 to 4 minutes. - The
denser substances 435 instratum 430 are decanted, drained, filtered, eliminated or otherwise removed fromsyringe 100 throughfilter 274 either during or after centrifugation. Thedenser substances 435 may be removed during centrifugation, provided thatassemblies 200 including filter caps 272 are coupled with thesyringes 100 before centrifugation ad thefilter 274 is not covered during centrifugation, as illustrated in FIGS. 16 to 19. The removeddenser substances 435 may pass throughfilter 274, into thedistal-most portion 332 ofinsert cavity 310. Rotation ofsyringe 100 provides a force acting radially outward 704 from a centralvertical axis 514 ofcentrifuge 500, leading thedenser substances 435 to travel, forming thedistal-most stratum 430 in theluer end 112 ofsyringe 100, which is angled further away fromaxis 514, and then further downward and outward throughfilter 274. - Upon contact with
material 360, e.g., an absorbent powder, indistal-most portion 332, removeddenser substances 435 may turn into a solid or gel-like substance 361 indistal-most portion 332 ofinsert cavity 310. The removeddenser substances 435 may remain in theinsert 300, as illustrated inFIG. 27 , and may be disposed with theinsert 300, after the removal from theinsert 300 ofsyringes 100 withstrata oil 415 andfat 425, andassemblies 200 coupled with the syringes. As described above, the off-center or offset position of opening 130 on theluer end 112 ofsyringe 100 allows for the elimination of more of thedenser substances 415 including the tumescent fluid fromsyringe 100 whensyringe 100 is in an angled vertical position during centrifugation, without trapping any residual substances or fluid in thechamber 120 ofsyringe 100, as illustrated inFIGS. 18 and 19 . - The
denser substances 435 instratum 430 may also be removed fromsyringe 100 throughfilter 274 after centrifugation. In this case, thefilter 274 is blocked withcover 275 during centrifugation, illustrated inFIGS. 5 and 6 . Oncesyringe 100 is stationary,cover 275 is removed, anddenser substances 435 are permitted to drain or be filtered out throughfilter 274. - Regardless of whether
denser substances 435 are removed during or after centrifugation,filter 274 does not permit passage offat 425 fromsyringe 100. In other words, oncedenser substances 435 are removed throughfilter 274, thefirst fat 425 contacting or touchingfilter 274 creates an airtight seal protecting the rest offat 425 from air contamination. -
Oil 415 is extracted or removed from theproximal stratum 410 insyringe 100 either during or after centrifugation, as illustrated inFIGS. 3 and 28 .Oil 415 may be removed during centrifugation if theplunger 160 includes afilter 169 covering opening 168 inplunger head 162, as described above, and as illustrated inFIG. 3 . Aclosure 167 may be provided to selectively close theopening 168 and block offfilter 169 during movement ofplunger 160 inproximal direction 700 to drawsubstances 400 intosyringe 100, and during movement ofplunger 160 indistal direction 702 to pushsubstances 400 out ofsyringe 100. In this case, during centrifugation, asdenser substances 435 are filtered out throughfilter 274 at theluer end 112 ofsyringe 100,oil 415 moves inproximal direction 700 throughfilter 169 and out ofsyringe 100. - However, operators may want to collect extracted or removed
oil 415 and use it for other purposes. In such a case, the syringe or insert may either have acollector 380 or another mechanism, component, device or member for collecting such extracted or removedoil 415, or the oil is extracted or removed after centrifugation in a more contained environment. - If operators prefer to extract or remove
oil 415 after centrifugation, than afilter 169 is not necessary on port orvalve 161. For removingoil 415 after centrifugation, the operator may first detach, disengage, decouple or otherwise remove insert 300 (withsyringes 100 and theirrespective assemblies 200 andsubstances 400 contained therein) fromrotatable centrifuge member 520,place insert 300 onsurface 800, before openinginsert cover 370, in order to maintain cleanliness ofcentrifuge 500, as illustrated inFIG. 27 . The operator opensinsert cover 370 and may operate onsyringe 100 whilesyringe 100 is retained ininsert cavity 310, as illustrated inFIG. 27 , or removessyringe 100 frominsert cavity 310, before extracting or removingoil 415 therefrom. Oncesyringe 100 andplunger head 162 is exposed, operator may aspirate or draw or extractoil 415 out from theproximal-most stratum 410 through aspiration valve orport 161 andopening 168, as illustrated inFIG. 28 . - The
syringe 100 then contains only fat 425. The operator may then use the same valve orport 161 andopening 168 to inject additional substances intosyringe 100, e.g., hormones or cleaning solutions which may be regarded as increasing the viability of the fat 425. These additional substances may be left mixed in withfat 425, or centrifuged out again, in a second centrifuging process. - Regardless of whether additional substances are introduced into
syringe 100, the fat 425 is then transferred to a series ofsmaller syringes 600, e.g., 1 cc syringes, for injection, as illustrated inFIG. 29 . Thefilter cap 274 illustrated inFIG. 5 or 6 is removed fromassembly 200 by either breaking it off fromadapter 230 or unscrewing it fromadapter 230, leavingsyringe 100 coupled withproximal end 232 ofadapter 230.Syringe 600 is coupled withadapter 230, as illustrated inFIG. 7 .Plunger shaft 164 is re-coupled or reattached withplunger head 162, and movement ofplunger 160 indistal direction 702 pushes fat 425 (and any additional substances, if any) out fromsyringe 100, throughpassageway 236 inadapter 230, and intosyringe 600, as illustrated inFIG. 29 . - The fat 425 is thus collected and prepared for being injected into the desired location with
syringe 600. - In the preceding specification, the present invention has been described with reference to specific exemplary embodiments thereof. It will, however, be evident that various modifications and changes may be made thereunto without departing from the broader spirit and scope of the present invention as set forth in the claims that follow. The specification and drawings are accordingly to be regarded in an illustrative rather than restrictive sense.
Claims (66)
Priority Applications (11)
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US10/732,125 US20050124073A1 (en) | 2003-12-09 | 2003-12-09 | Fat collection and preparation system and method |
US10/844,129 US7306740B2 (en) | 2003-12-09 | 2004-05-11 | Fat collection and preparation system and method |
AU2004296873A AU2004296873B2 (en) | 2003-12-09 | 2004-12-09 | Fat collection and preparation system and method |
BRPI0417131-4A BRPI0417131A2 (en) | 2003-12-09 | 2004-12-09 | fat collection and preparation system and method |
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JP2006544012A JP2007537773A (en) | 2003-12-09 | 2004-12-09 | Fat collection and preparation system and method |
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RU2006124015/14A RU2392869C2 (en) | 2003-12-09 | 2004-12-09 | Method of fat preparation for transplantation |
CA002563543A CA2563543A1 (en) | 2003-12-09 | 2004-12-09 | Fat collection and preparation system and method |
US11/738,950 US7488427B2 (en) | 2003-12-09 | 2007-04-23 | Fat collection and preparation system and method |
US11/937,187 US20080146917A1 (en) | 2003-12-09 | 2007-11-08 | Fat Collection and Preparation System and Method |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050282291A1 (en) * | 2004-06-18 | 2005-12-22 | Pankratz Thomas J R | Method for aspiration of a liquid sample |
US20060051252A1 (en) * | 2004-09-09 | 2006-03-09 | Microfluidic Systems, Inc. | Handheld and portable microfluidic device to automatically prepare nucleic acids for analysis |
US20060246501A1 (en) * | 2004-09-09 | 2006-11-02 | Microfluidic Systems, Inc. | Apparatus and method of extracting and optically analyzing an analyte from a fluid-based sample |
US7976796B1 (en) * | 2008-07-03 | 2011-07-12 | Emcyte Corp. | Centrifuge tube for separating and aspirating biological components |
US20110309037A1 (en) * | 2004-06-23 | 2011-12-22 | Hee Young Lee | Method of Separating Free Oil from Fat |
US20120053041A1 (en) * | 2009-10-28 | 2012-03-01 | Glotech Co., Ltd. | Centrifugal separation kit and methods for centrifugal separation using the same |
US20160333305A1 (en) * | 2015-05-15 | 2016-11-17 | Black Tie Medical Inc. | Device and Method for Breaking Down and Sizing Harvested Fat |
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Families Citing this family (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2004296873B2 (en) * | 2003-12-09 | 2009-10-08 | Lipose Corporation | Fat collection and preparation system and method |
WO2009148969A1 (en) | 2008-06-02 | 2009-12-10 | Sta-Med, Llc | Needle cover assembly for a syringe |
US8446586B2 (en) * | 2008-10-15 | 2013-05-21 | Allan Yang Wu | Method and apparatus for increasing adipose vascular fraction |
US8309343B2 (en) | 2008-12-01 | 2012-11-13 | Baxter International Inc. | Apparatus and method for processing biological material |
US8100874B1 (en) | 2009-05-22 | 2012-01-24 | Donnell Mark Jordan | Tissue refining device |
US8540078B2 (en) * | 2009-06-16 | 2013-09-24 | Biomet Biologics, Llc | Liquid separation from adipose tissue |
US8790519B2 (en) * | 2009-06-16 | 2014-07-29 | Biomet Biologics, Llc | Liquid separation from adipose tissue |
US8465471B2 (en) | 2009-08-05 | 2013-06-18 | Rocin Laboratories, Inc. | Endoscopically-guided electro-cauterizing power-assisted fat aspiration system for aspirating visceral fat tissue within the abdomen of a patient |
US20110213336A1 (en) | 2009-08-05 | 2011-09-01 | Cucin Robert L | Method of and apparatus for sampling, processing and collecting tissue and reinjecting the same into human patients |
US8348929B2 (en) | 2009-08-05 | 2013-01-08 | Rocin Laboratories, Inc. | Endoscopically-guided tissue aspiration system for safely removing fat tissue from a patient |
US8986185B2 (en) * | 2009-09-24 | 2015-03-24 | Lipovera, Llc | Syringe centrifuge systems |
US7927563B1 (en) * | 2009-10-13 | 2011-04-19 | Cytomedix, Inc. | Kit for separation of biological fluids |
US20110086426A1 (en) * | 2009-10-13 | 2011-04-14 | Lipostem Corp. | Methods and apparatus for collecting and separating regenerative cells from adipose tissue |
US8162882B2 (en) | 2010-06-23 | 2012-04-24 | Sta-Med, Llc | Automatic-locking safety needle covers and methods of use and manufacture |
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US8870733B2 (en) | 2010-11-19 | 2014-10-28 | Kensey Nash Corporation | Centrifuge |
AU2011343513B2 (en) * | 2010-12-16 | 2016-07-14 | Ingeneron, Inc. | Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples |
US20130343967A1 (en) * | 2011-01-06 | 2013-12-26 | Lacerta Technologies Inc. | Device for centrifugation and further processing of tissue aspirates in closed system |
ES1073745Y (en) * | 2011-01-19 | 2011-05-06 | Ventosa Enric Jorda | DEVICE FOR EXTRACTION AND OBTAINING BLOOD PLASMA AND / OR FAT |
WO2012166746A1 (en) | 2011-05-31 | 2012-12-06 | Sta-Med, Llc | Blood collection safety devices and methods of use and manufacture |
WO2013051115A1 (en) * | 2011-10-05 | 2013-04-11 | スーガン株式会社 | Injector head with rotating mechanism |
FR2981852B1 (en) * | 2011-10-31 | 2014-12-05 | Symatese | DEVICE FOR THE COLLECTION AND REINJECTION OF A FLUID, INJECTOR AND ASSOCIATED SAMPLING NEEDS |
US10464060B2 (en) * | 2011-11-10 | 2019-11-05 | BioFare Diagnostics, LLC | Loading vials |
CN102961805A (en) * | 2012-06-18 | 2013-03-13 | 韶山市夏龙医疗器械制造有限公司 | Self-purified fat quantitative transplanting device |
US20140081237A1 (en) * | 2012-09-20 | 2014-03-20 | Tissue Genesis, Inc. | Hand-held micro-liposuction adipose harvester, processor, and cell concentrator |
US11821824B2 (en) * | 2012-09-20 | 2023-11-21 | Tissue Genesis International Llc | Cell separation apparatus |
US9333447B2 (en) * | 2012-10-19 | 2016-05-10 | Warsaw Orthopedic, Inc. | Filtration device |
WO2014164815A2 (en) * | 2013-03-12 | 2014-10-09 | Allergan, Inc. | Adipose tissue combinations, devices, and uses thereof |
CA2947955C (en) * | 2013-05-17 | 2020-07-07 | Carrtech Llc | Filtering needle cap |
US20140350516A1 (en) | 2013-05-23 | 2014-11-27 | Allergan, Inc. | Mechanical syringe accessory |
US9248384B2 (en) | 2013-10-02 | 2016-02-02 | Allergan, Inc. | Fat processing system |
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US10029048B2 (en) | 2014-05-13 | 2018-07-24 | Allergan, Inc. | High force injection devices |
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Citations (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3596652A (en) * | 1968-10-14 | 1971-08-03 | Bio Science Labor | Fluid separatory device |
US3750645A (en) * | 1970-10-20 | 1973-08-07 | Becton Dickinson Co | Method of collecting blood and separating cellular components thereof |
US3817389A (en) * | 1973-01-15 | 1974-06-18 | Sherwood Medical Ind Inc | Filter device in tubular fitting for medical injection equipment and the like |
US3865731A (en) * | 1973-12-21 | 1975-02-11 | Baxter Laboratories Inc | Filter skimming device |
US4014797A (en) * | 1973-12-11 | 1977-03-29 | Burron Medical Products, Inc. | Intravenous injection apparatus and needle adapter with filter and method of making same |
US4119542A (en) * | 1974-10-18 | 1978-10-10 | Matsushita Electric Industrial Company, Limited | Centrifugal separator |
US4316462A (en) * | 1980-05-21 | 1982-02-23 | Siloam, Inc. | Filtering device for an injection device |
US4364832A (en) * | 1981-01-21 | 1982-12-21 | Ballies Uwe W | Separating member in a separating tube for centrifugal separation |
US4391274A (en) * | 1981-06-26 | 1983-07-05 | Becton, Dickinson And Company | Filtered hub device for aspirating and injecting liquids |
US4448206A (en) * | 1981-08-17 | 1984-05-15 | Martell Michael D | Vented, aspirating syringe |
US4632672A (en) * | 1985-10-07 | 1986-12-30 | Minnesota Mining And Manufacturing Company | Self venting syringe plunger |
US4660569A (en) * | 1986-02-10 | 1987-04-28 | Sealsyringe Corporation | Venting, automatic-stopping, aspirating plungers for syringes |
US4702162A (en) * | 1987-04-14 | 1987-10-27 | Cuisinarts, Inc. | Salad spinner dryer apparatus rotated by speed reducing friction drive for use wih food processors |
US4781683A (en) * | 1987-04-22 | 1988-11-01 | The Johns Hopkins University | Single-use, self-annulling injection syringe |
US4820276A (en) * | 1988-02-08 | 1989-04-11 | Enrique Moreno | Filter assembly for use with a hypodermic syringe |
US4958557A (en) * | 1989-10-17 | 1990-09-25 | Jeffrey Fiala | Rendered fat separator |
US5002538A (en) * | 1988-10-25 | 1991-03-26 | Johnson Johnnie M | Syringe adapter and method |
US5125415A (en) * | 1990-06-19 | 1992-06-30 | Smiths Industries Medical Systems, Inc. | Syringe tip cap with self-sealing filter |
US5149432A (en) * | 1991-04-30 | 1992-09-22 | Aaron Lavin | Centrifuge for separating liquids of different specific gravities |
US5238003A (en) * | 1992-02-07 | 1993-08-24 | Baidwan Balinderjeet S | Plunger tip for blood gas syringe |
US5255688A (en) * | 1989-09-27 | 1993-10-26 | Bredam Medical Distribution S.A. | Instrument for handling biopsies and specimens |
US5441487A (en) * | 1993-11-30 | 1995-08-15 | Medex, Inc. | Plastic needleless valve housing for standard male luer locks |
US5474675A (en) * | 1988-11-17 | 1995-12-12 | Herco-Cff Chiralflow Filtertechnik Gmbh | Filter separator for separating a composite fluid |
US5480484A (en) * | 1994-03-01 | 1996-01-02 | Statspin Technologies | Cytology centrifuge apparatus |
US5489266A (en) * | 1994-01-25 | 1996-02-06 | Becton, Dickinson And Company | Syringe assembly and method for lyophilizing and reconstituting injectable medication |
US5490453A (en) * | 1995-01-09 | 1996-02-13 | Sas Tv Products, Inc. | Centrifugal fat extraction apparatus |
US5624402A (en) * | 1994-12-12 | 1997-04-29 | Becton, Dickinson And Company | Syringe tip cap |
US5679154A (en) * | 1994-03-01 | 1997-10-21 | Norfolk Scientific, Inc. | Cytology centrifuge apparatus |
US5685864A (en) * | 1993-11-22 | 1997-11-11 | Interventional Research Technologies | Locking device for aspirator, syringe or like instrument |
US5795477A (en) * | 1995-01-25 | 1998-08-18 | Fleetguard, Inc. | Self-driven, cone-stack type centrifuge |
US5807353A (en) * | 1996-09-16 | 1998-09-15 | Schmitz; Thomas David | Aspiration device with separating element |
US5830359A (en) * | 1993-04-07 | 1998-11-03 | Knight Scientific Limited | Method of separating particles from a filter |
US5851201A (en) * | 1996-01-19 | 1998-12-22 | Acacia Laboratories, Inc. | Luer connector |
US5855230A (en) * | 1995-04-06 | 1999-01-05 | Industrie Barla Spa | Luer-lock fitting for medical infusion/tranfusion lines |
US5865803A (en) * | 1997-05-19 | 1999-02-02 | Major; Miklos | Syringe device having a vented piston |
US5919169A (en) * | 1997-06-23 | 1999-07-06 | Grams; Guenter | Cannula lock and seal mechanism |
US20010035101A1 (en) * | 2000-03-31 | 2001-11-01 | Bruce Ancona | Electric salad spinner |
US6383173B1 (en) * | 1999-10-22 | 2002-05-07 | Roy H. Hunt | Syringe drench adapter for livestock |
US20020143298A1 (en) * | 2001-03-30 | 2002-10-03 | Becton, Dickinson And Company | Blunt cannula and filter assembly and method of use with point-of-care testing cartridge |
US6471069B2 (en) * | 1999-12-03 | 2002-10-29 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US20020182664A1 (en) * | 2001-04-09 | 2002-12-05 | Dolecek Victor D. | Methods of isolating blood components using a microcentrifuge and uses thereof |
US6494896B1 (en) * | 1999-11-30 | 2002-12-17 | Closure Medical Corporation | Applicator for laparoscopic or endoscopic surgery |
US6520935B1 (en) * | 1994-12-12 | 2003-02-18 | Becton, Dickinson And Company | Syringe and tip cap assembly |
US20030069543A1 (en) * | 2001-07-10 | 2003-04-10 | Carpenter Kenneth W. | Sterile aspiration/reinjection systems |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5100564A (en) | 1990-11-06 | 1992-03-31 | Pall Corporation | Blood collection and processing system |
AU6542090A (en) | 1989-10-19 | 1991-05-16 | Jeffrey Fiala | Rendered fat separator |
-
2003
- 2003-12-09 US US10/732,125 patent/US20050124073A1/en not_active Abandoned
-
2004
- 2004-05-11 US US10/844,129 patent/US7306740B2/en not_active Expired - Fee Related
- 2004-12-09 BR BRPI0417131-4A patent/BRPI0417131A2/en not_active Application Discontinuation
-
2007
- 2007-04-23 US US11/738,950 patent/US7488427B2/en not_active Expired - Fee Related
Patent Citations (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3596652A (en) * | 1968-10-14 | 1971-08-03 | Bio Science Labor | Fluid separatory device |
US3750645A (en) * | 1970-10-20 | 1973-08-07 | Becton Dickinson Co | Method of collecting blood and separating cellular components thereof |
US3817389A (en) * | 1973-01-15 | 1974-06-18 | Sherwood Medical Ind Inc | Filter device in tubular fitting for medical injection equipment and the like |
US4014797A (en) * | 1973-12-11 | 1977-03-29 | Burron Medical Products, Inc. | Intravenous injection apparatus and needle adapter with filter and method of making same |
US3865731A (en) * | 1973-12-21 | 1975-02-11 | Baxter Laboratories Inc | Filter skimming device |
US4119542A (en) * | 1974-10-18 | 1978-10-10 | Matsushita Electric Industrial Company, Limited | Centrifugal separator |
US4316462A (en) * | 1980-05-21 | 1982-02-23 | Siloam, Inc. | Filtering device for an injection device |
US4364832A (en) * | 1981-01-21 | 1982-12-21 | Ballies Uwe W | Separating member in a separating tube for centrifugal separation |
US4391274A (en) * | 1981-06-26 | 1983-07-05 | Becton, Dickinson And Company | Filtered hub device for aspirating and injecting liquids |
US4448206A (en) * | 1981-08-17 | 1984-05-15 | Martell Michael D | Vented, aspirating syringe |
US4632672A (en) * | 1985-10-07 | 1986-12-30 | Minnesota Mining And Manufacturing Company | Self venting syringe plunger |
US4660569A (en) * | 1986-02-10 | 1987-04-28 | Sealsyringe Corporation | Venting, automatic-stopping, aspirating plungers for syringes |
US4702162A (en) * | 1987-04-14 | 1987-10-27 | Cuisinarts, Inc. | Salad spinner dryer apparatus rotated by speed reducing friction drive for use wih food processors |
US4781683A (en) * | 1987-04-22 | 1988-11-01 | The Johns Hopkins University | Single-use, self-annulling injection syringe |
US4820276A (en) * | 1988-02-08 | 1989-04-11 | Enrique Moreno | Filter assembly for use with a hypodermic syringe |
US5002538A (en) * | 1988-10-25 | 1991-03-26 | Johnson Johnnie M | Syringe adapter and method |
US5474675A (en) * | 1988-11-17 | 1995-12-12 | Herco-Cff Chiralflow Filtertechnik Gmbh | Filter separator for separating a composite fluid |
US5255688A (en) * | 1989-09-27 | 1993-10-26 | Bredam Medical Distribution S.A. | Instrument for handling biopsies and specimens |
US4958557A (en) * | 1989-10-17 | 1990-09-25 | Jeffrey Fiala | Rendered fat separator |
US5125415A (en) * | 1990-06-19 | 1992-06-30 | Smiths Industries Medical Systems, Inc. | Syringe tip cap with self-sealing filter |
US5149432A (en) * | 1991-04-30 | 1992-09-22 | Aaron Lavin | Centrifuge for separating liquids of different specific gravities |
US5238003A (en) * | 1992-02-07 | 1993-08-24 | Baidwan Balinderjeet S | Plunger tip for blood gas syringe |
US5830359A (en) * | 1993-04-07 | 1998-11-03 | Knight Scientific Limited | Method of separating particles from a filter |
US5685864A (en) * | 1993-11-22 | 1997-11-11 | Interventional Research Technologies | Locking device for aspirator, syringe or like instrument |
US5441487A (en) * | 1993-11-30 | 1995-08-15 | Medex, Inc. | Plastic needleless valve housing for standard male luer locks |
US5489266A (en) * | 1994-01-25 | 1996-02-06 | Becton, Dickinson And Company | Syringe assembly and method for lyophilizing and reconstituting injectable medication |
US5679154A (en) * | 1994-03-01 | 1997-10-21 | Norfolk Scientific, Inc. | Cytology centrifuge apparatus |
US5480484A (en) * | 1994-03-01 | 1996-01-02 | Statspin Technologies | Cytology centrifuge apparatus |
US6190364B1 (en) * | 1994-12-12 | 2001-02-20 | Becton, Dickinson And Company | Syringe tip cap |
US5624402A (en) * | 1994-12-12 | 1997-04-29 | Becton, Dickinson And Company | Syringe tip cap |
US6520935B1 (en) * | 1994-12-12 | 2003-02-18 | Becton, Dickinson And Company | Syringe and tip cap assembly |
US5490453A (en) * | 1995-01-09 | 1996-02-13 | Sas Tv Products, Inc. | Centrifugal fat extraction apparatus |
US5795477A (en) * | 1995-01-25 | 1998-08-18 | Fleetguard, Inc. | Self-driven, cone-stack type centrifuge |
US5855230A (en) * | 1995-04-06 | 1999-01-05 | Industrie Barla Spa | Luer-lock fitting for medical infusion/tranfusion lines |
US5851201A (en) * | 1996-01-19 | 1998-12-22 | Acacia Laboratories, Inc. | Luer connector |
US5807353A (en) * | 1996-09-16 | 1998-09-15 | Schmitz; Thomas David | Aspiration device with separating element |
US5865803A (en) * | 1997-05-19 | 1999-02-02 | Major; Miklos | Syringe device having a vented piston |
US5919169A (en) * | 1997-06-23 | 1999-07-06 | Grams; Guenter | Cannula lock and seal mechanism |
US6383173B1 (en) * | 1999-10-22 | 2002-05-07 | Roy H. Hunt | Syringe drench adapter for livestock |
US6494896B1 (en) * | 1999-11-30 | 2002-12-17 | Closure Medical Corporation | Applicator for laparoscopic or endoscopic surgery |
US6471069B2 (en) * | 1999-12-03 | 2002-10-29 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US20010035101A1 (en) * | 2000-03-31 | 2001-11-01 | Bruce Ancona | Electric salad spinner |
US20020143298A1 (en) * | 2001-03-30 | 2002-10-03 | Becton, Dickinson And Company | Blunt cannula and filter assembly and method of use with point-of-care testing cartridge |
US20020182664A1 (en) * | 2001-04-09 | 2002-12-05 | Dolecek Victor D. | Methods of isolating blood components using a microcentrifuge and uses thereof |
US20030069543A1 (en) * | 2001-07-10 | 2003-04-10 | Carpenter Kenneth W. | Sterile aspiration/reinjection systems |
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---|---|---|---|---|
US7205158B2 (en) * | 2004-06-18 | 2007-04-17 | Dade Behring Inc. | Method for aspiration of a liquid sample |
US20050282291A1 (en) * | 2004-06-18 | 2005-12-22 | Pankratz Thomas J R | Method for aspiration of a liquid sample |
US8926487B2 (en) * | 2004-06-23 | 2015-01-06 | Hee Young Lee | Method of separating free oil from fat |
US20110309037A1 (en) * | 2004-06-23 | 2011-12-22 | Hee Young Lee | Method of Separating Free Oil from Fat |
US20060051252A1 (en) * | 2004-09-09 | 2006-03-09 | Microfluidic Systems, Inc. | Handheld and portable microfluidic device to automatically prepare nucleic acids for analysis |
US20060246501A1 (en) * | 2004-09-09 | 2006-11-02 | Microfluidic Systems, Inc. | Apparatus and method of extracting and optically analyzing an analyte from a fluid-based sample |
US7988935B2 (en) * | 2004-09-09 | 2011-08-02 | Microfluidic Systems, Inc. | Handheld and portable microfluidic device to automatically prepare nucleic acids for analysis |
US8053214B2 (en) | 2004-09-09 | 2011-11-08 | Microfluidic Systems, Inc. | Apparatus and method of extracting and optically analyzing an analyte from a fluid-based sample |
US7976796B1 (en) * | 2008-07-03 | 2011-07-12 | Emcyte Corp. | Centrifuge tube for separating and aspirating biological components |
US20120053041A1 (en) * | 2009-10-28 | 2012-03-01 | Glotech Co., Ltd. | Centrifugal separation kit and methods for centrifugal separation using the same |
US9329165B2 (en) * | 2009-10-28 | 2016-05-03 | Glotech Co., Ltd. | Centrifugal separation kit and methods for centrifugal separation using the same |
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Also Published As
Publication number | Publication date |
---|---|
US20080057597A1 (en) | 2008-03-06 |
US7306740B2 (en) | 2007-12-11 |
BRPI0417131A2 (en) | 2009-03-10 |
US20050123895A1 (en) | 2005-06-09 |
US7488427B2 (en) | 2009-02-10 |
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