US20050100637A1 - Carbohydrate and electrolyte replacement composition - Google Patents
Carbohydrate and electrolyte replacement composition Download PDFInfo
- Publication number
- US20050100637A1 US20050100637A1 US10/706,420 US70642003A US2005100637A1 US 20050100637 A1 US20050100637 A1 US 20050100637A1 US 70642003 A US70642003 A US 70642003A US 2005100637 A1 US2005100637 A1 US 2005100637A1
- Authority
- US
- United States
- Prior art keywords
- meq
- beverage
- sodium
- composition
- chloride
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 308
- 150000001720 carbohydrates Chemical class 0.000 title claims abstract description 75
- 239000003792 electrolyte Substances 0.000 title claims abstract description 27
- 235000014633 carbohydrates Nutrition 0.000 claims abstract description 75
- 230000000694 effects Effects 0.000 claims abstract description 71
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 67
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 52
- 230000018044 dehydration Effects 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 40
- 239000000796 flavoring agent Substances 0.000 claims abstract description 35
- 235000013355 food flavoring agent Nutrition 0.000 claims abstract description 24
- 235000013361 beverage Nutrition 0.000 claims description 281
- 239000011734 sodium Substances 0.000 claims description 185
- 229910052708 sodium Inorganic materials 0.000 claims description 182
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 180
- 239000012530 fluid Substances 0.000 claims description 111
- 239000012141 concentrate Substances 0.000 claims description 81
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 78
- 239000011591 potassium Substances 0.000 claims description 78
- 229910052700 potassium Inorganic materials 0.000 claims description 78
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 75
- 239000011777 magnesium Substances 0.000 claims description 51
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 49
- 229910052749 magnesium Inorganic materials 0.000 claims description 49
- 239000011575 calcium Substances 0.000 claims description 46
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 44
- 229910052791 calcium Inorganic materials 0.000 claims description 44
- 239000007788 liquid Substances 0.000 claims description 39
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 34
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 34
- 150000002500 ions Chemical class 0.000 claims description 31
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 27
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 27
- 239000008103 glucose Substances 0.000 claims description 27
- 210000003722 extracellular fluid Anatomy 0.000 claims description 25
- 239000003795 chemical substances by application Substances 0.000 claims description 24
- 229930091371 Fructose Natural products 0.000 claims description 21
- 239000005715 Fructose Substances 0.000 claims description 21
- 229930006000 Sucrose Natural products 0.000 claims description 18
- 239000005720 sucrose Substances 0.000 claims description 18
- 239000011780 sodium chloride Substances 0.000 claims description 17
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 15
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 15
- 239000001509 sodium citrate Substances 0.000 claims description 15
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 15
- 229920002774 Maltodextrin Polymers 0.000 claims description 14
- 239000005913 Maltodextrin Substances 0.000 claims description 14
- 229940035034 maltodextrin Drugs 0.000 claims description 14
- 230000002485 urinary effect Effects 0.000 claims description 14
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 13
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 13
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 13
- 229920002498 Beta-glucan Polymers 0.000 claims description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 13
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 13
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 13
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 13
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims description 13
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 13
- 229930182830 galactose Natural products 0.000 claims description 13
- 150000003641 trioses Chemical class 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 125000000185 sucrose group Chemical group 0.000 claims 9
- 230000002708 enhancing effect Effects 0.000 claims 4
- 230000002411 adverse Effects 0.000 abstract description 3
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 description 148
- 238000009472 formulation Methods 0.000 description 139
- 210000004243 sweat Anatomy 0.000 description 61
- 238000011084 recovery Methods 0.000 description 58
- 238000011282 treatment Methods 0.000 description 53
- 238000012360 testing method Methods 0.000 description 43
- 235000019600 saltiness Nutrition 0.000 description 40
- 230000037396 body weight Effects 0.000 description 39
- 230000001953 sensory effect Effects 0.000 description 38
- 238000002474 experimental method Methods 0.000 description 35
- 239000000523 sample Substances 0.000 description 35
- 230000035622 drinking Effects 0.000 description 20
- 230000000717 retained effect Effects 0.000 description 20
- 230000036571 hydration Effects 0.000 description 17
- 238000006703 hydration reaction Methods 0.000 description 17
- 235000013305 food Nutrition 0.000 description 16
- 230000002747 voluntary effect Effects 0.000 description 16
- 229910001415 sodium ion Inorganic materials 0.000 description 15
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 13
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 12
- 235000005911 diet Nutrition 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 11
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- 235000019634 flavors Nutrition 0.000 description 11
- 230000005484 gravity Effects 0.000 description 11
- 230000004044 response Effects 0.000 description 11
- 235000011496 sports drink Nutrition 0.000 description 11
- 229960005069 calcium Drugs 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 235000008504 concentrate Nutrition 0.000 description 9
- 230000037213 diet Effects 0.000 description 9
- 235000012054 meals Nutrition 0.000 description 9
- 230000008901 benefit Effects 0.000 description 8
- 230000008859 change Effects 0.000 description 8
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 8
- 238000011156 evaluation Methods 0.000 description 8
- 230000002496 gastric effect Effects 0.000 description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 description 8
- 235000010755 mineral Nutrition 0.000 description 8
- 239000011707 mineral Substances 0.000 description 8
- 230000000276 sedentary effect Effects 0.000 description 8
- 235000002639 sodium chloride Nutrition 0.000 description 8
- 230000007937 eating Effects 0.000 description 7
- 206010013911 Dysgeusia Diseases 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 6
- 235000021152 breakfast Nutrition 0.000 description 6
- 229960001948 caffeine Drugs 0.000 description 6
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 6
- 230000009429 distress Effects 0.000 description 6
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- 230000001351 cycling effect Effects 0.000 description 5
- 235000021023 sodium intake Nutrition 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 206010028813 Nausea Diseases 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 229940109239 creatinine Drugs 0.000 description 4
- 238000013480 data collection Methods 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 238000002637 fluid replacement therapy Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
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- 230000035922 thirst Effects 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 102000001554 Hemoglobins Human genes 0.000 description 3
- 108010054147 Hemoglobins Proteins 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000036626 alertness Effects 0.000 description 3
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- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
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- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- RYCLIXPGLDDLTM-UHFFFAOYSA-J tetrapotassium;phosphonato phosphate Chemical compound [K+].[K+].[K+].[K+].[O-]P([O-])(=O)OP([O-])([O-])=O RYCLIXPGLDDLTM-UHFFFAOYSA-J 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical class [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 235000013337 tricalcium citrate Nutrition 0.000 description 1
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Images
Classifications
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention relates to an improved rehydration beverage which provides for fluid, carbohydrate, and electrolyte replacement.
- the beverage comprised of a novel mineral blend enhances rehydration by providing superior voluntary fluid consumption and fluid retention.
- Non-fluid components of the beverage composition can be provided in forms other than beverages.
- Rehydration can be accomplished in a number of different ways. In the most basic sense, water replaces some of the fluids lost through sweat and helps maintain body temperature and important cardiovascular functions. Sports drinks have been developed which replace the fluids and electrolytes lost through sweat. This is an improvement over plain water not only because these drinks replace some of the minerals lost in sweat but also because carbohydrates are provided as a source of added energy. However, rehydration would be improved if the beverage also improved fluid retention, reduced urinary fluid loss, stimulated increased voluntary consumption, possessed superior sensory qualities, and supported the physiological response to continue drinking.
- the mineral content of sports drinks varies widely. For instance some beverages can contain 5 mEq/L of sodium while others can contain over four times that amount. Similarly, there are wide differences in the content and amounts of other minerals, such as potassium, magnesium and chloride.
- Fluid replacement after significant dehydration is driven by various physiological changes.
- the two major physiological drivers that encourage voluntary drinking are plasma osmolality and plasma volume.
- plasma osmolality and plasma volume During exercise, the loss of fluid through sweat causes plasma volume to drop and plasma osmolality to increase. These physiological changes cause a thirst response which drives voluntary fluid consumption.
- Scientific studies have shown that sodium also plays an important role in regulating plasma volume and osmolality. Ingesting beverages containing sodium helps increase the rate at which plasma volume and osmolality return to normal. However, ingesting too high a level of sodium causes rapid restoration of plasma volume, which reduces the drinking response and prevents adequate rehydration.
- the sensory properties of a beverage containing too high a level of sodium are unfavorable, and would further reduce the drive to drink.
- electrolytes and minerals play an important role in rehydration by possibly affecting fluid replacement and fluid retention.
- water is distributed between fluid compartments so that both the extracellular and intracellular compartments share the water deficit.
- Sodium, potassium, magnesium, calcium and chloride are some of the more important electrolytes/minerals involved in filling these body fluid compartments, particularly sodium, chloride, potassium and magnesium. Beverages providing sodium and chloride encourage the filling of the extracellular compartment, while beverages providing potassium, magnesium, and calcium favor the filling of the intracellular compartment. Properly balancing the sodium, potassium, magnesium, calcium and chloride levels will further improve the rehydration properties of the beverage.
- electrolyte ions assist in filling these body fluid compartments more rapidly and help to retain the fluid instead of it being excreted as urine. Since both sodium and chloride ions favor the filling of the extracellular compartments, substitution of one with the other may not affect the overall result. The same may be true for potassium and magnesium in regards to intracellular hydration.
- U.S. Pat. No. 4,981,687 issued to Fregly et al., incorporated herein, discloses a beverage comprising water, sugar, and electrolytes, an improvement wherein said beverage further comprises glycerol, pyruvate and/or caffeine.
- the sugar contained in the beverage claimed in this patent can be glucose, sucrose or other appropriate sugar compound, with glucose at a concentration of from about 2% to about 8% being specifically disclosed and glucose at a concentration of about 4% being preferred.
- Examples of electrolytes disclosed in this patent include 15-30 mEq/L sodium, 1-5 mEq/L potassium, 2-8 mEq/L phosphate, bicarbonate, sulfate, chloride, calcium, and magnesium.
- the beverages as disclosed above are said to ameliorate the adverse physiological effects which can result from physical exertion and heat exposure.
- the present invention also seeks to address the adverse physiological effects of physical exertion, but without including stimulants or other chemical compounds which may have known acute effects (e.g. glycerol and pyruvate promoting gastrointestinal distress) and unknown long-term effects.
- the present invention fulfills these needs.
- the beverages of the present invention enhance rehydration, supply necessary electrolytes and energy sources, exhibit organoleptic properties at least substantially equivalent to other sports drinks, improve fluid retention and voluntary fluid consumption.
- the method of the present invention also addresses the above concerns through the administration of the composition of the present invention.
- the composition may be administered orally.
- the composition can take many forms including but not limited to, liquid, gel, dry powder, tablet or capsule. Concentrated forms of the composition such as a powder can be can be added to water and/or other liquids which can include electrolytes and/or carbohydrates, including even sports drinks such as Gatorade® in order to provide beverages of the present invention.
- Example 2 Dehydration is reduced and fluid retention is improved by abating urinary loss when one embodiment of the present invention is taken during exercise, as illustrated in Example 2 hereof.
- Examples 3 through 7 further show that the present invention reduces the effects of dehydration, improves fluid retention, reduces urinary fluid loss, and possess superior sensory properties which should improve voluntary fluid consumption when taken after activity-induced fluid loss. It is further believed that similar results should be obtained when the beverage of the present invention is taken before activity-induced fluid loss.
- the present invention relates to a beverage composition for oral consumption comprising: from about 4% to about 10% by weight of carbohydrates; at least about 30 mEq/L of beverage of sodium; at least about 7 mEq/L of beverage of potassium; from about 10 to about 20 mEq/L of beverage of chloride; from about 0% to about 0.4% of a flavoring agent, when present; from about 0 to about 100 parts per million (ppm) of a clouding agent, when present; from about 0.24% to about 0.38% by weight citric acid, when present; and typically balance water.
- the extracellular-favoring ions may be present in combination at levels of from about 40 to about 78 mEq/L of beverage.
- the complete beverage can be fully formulated as noted above, or it can be made up by providing portions or all of a component or components by added liquids, whether the liquid is water or water already containing components adequate to prepare the final beverage.
- the formulation added to the liquid has components so as to make up the finally prepared beverage when the formulation and the liquid are combined to form the complete beverage.
- the osmolality of the beverage is in the range of from about 250 to about 350 mOsm/Kg.
- the beverage also may include from about 1 to about 6 mEq/L of calcium; and when present, from about 1 to about 6 mEq/L of magnesium.
- the present invention also relates to a beverage concentrate which is formulated to provide the beverage already described herein upon preparation by the consumer.
- the concentrate can take many forms including, but not limited to, gel, dry powder, tablet, capsule and liquid concentrates.
- the concentrates may be added to water and/or other liquids including water and carbohydrates and/or electrolytes, such as Gatorade®.
- Administration can be oral, intravenously, or by other suitable means.
- FIG. 1 shows the hedonic scores of various formulations with regard to overall acceptance and liking of saltiness when consumed during exercise.
- FIG. 2 illustrates the experimental procedure employed to determine ad-libitum drinking characteristics, sensory aspects, hydration characteristics, and voluntary fluid intake of various formulations.
- FIG. 3 shows the total fluid consumed for each of various formulations.
- FIG. 4 shows the trend in urine loss by graphing the urine volume for each formulation.
- FIG. 5 shows dehydration in terms of initial body weight of individuals to whom each formulation was administered.
- FIG. 6 illustrates the ratings of saltiness intensity during exercise and sedentary conditions and further shows the ideal perceived saltiness.
- FIG. 7 shows hedonic scores in terms of overall acceptance and liking of saltiness for the various formulations during exercise and sedentary conditions.
- FIG. 8 illustrates the experimental protocol used to determine how much fluid is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss.
- FIG. 9 shows cumulative urine loss over time during the recovery period for the various formulations.
- FIG. 10 shows fluid retention as a percentage of the volume ingested during recovery time for the various formulations.
- FIG. 11 illustrates the experimental protocol used to determine how much fluid is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss.
- FIG. 12 shows cumulative urine loss over time during the recovery period for various formulations.
- FIG. 13 shows fluid retention as a percentage of the volume ingested during recovery time for various formulations.
- FIG. 14 shows the plasma volume changes over time for various formulations.
- FIG. 15 shows fluid retention as a percentage of the volume ingested during recovery time for various formulations.
- FIG. 16 shows urine production over time during a recovery period for various formulations.
- FIG. 17 shows the urine osmolality over time-before exercise and during recovery-for various formulations.
- FIG. 18 shows urine sodium losses over time for various formulations.
- FIG. 19 shows urine potassium losses over time for various formulations.
- FIG. 20 shows urine specific gravity over time for various formulations.
- FIG. 21 illustrates the experimental protocol used to determine how much fluid is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss.
- FIG. 22 shows the percentage of fluid retained over time of the various formulations.
- FIG. 23 shows the cumulative urine loss in grams over time of the various formulations.
- FIG. 24 indicates the overall acceptance of the various formulations during the rehydration period preceding the measurement of fluid retention.
- FIG. 25 indicates the tartness scores of the various formulations during the rehydration period preceding the measurement of fluid retention.
- FIG. 26 indicates the perceived saltiness of the various formulations during the rehydration period preceding the measurement of fluid retention.
- FIG. 27 shows the percent fluid retained as a percentage of the volume ingested during recovery time for various formulations and historical range of fluid retention.
- FIG. 28 shows the urine osmolality over time-before exercise and during recovery-for various formulations.
- FIG. 29 shows the urine sodium concentration during the recovery period for various formulations.
- FIG. 30 shows the urine specific gravity over time-before and after exercise and during recovery-for various formulations.
- FIG. 31 shows the percent of total fluid retained at the end of the recovery period for various formulations.
- FIG. 32 shows the urine osmolality over time-before exercise and during recovery-for various formulations.
- FIG. 33 shows the urine specific gravity over time-before and after exercise and during recovery-for various formulations.
- FIG. 34 shows the urine potassium concentration during the recovery period for various formulations.
- FIG. 35 shows the overall acceptance ratings from the sensory evaluations for various formulations.
- the beverage of the present invention provides superior rehydration by improving fluid retention and reducing urine fluid loss. Additionally, the beverage enhances rehydration by also improving voluntary fluid intake. Moreover, the organoleptic or sensory properties of the beverage are at least as favorable as other sports drinks.
- the beverage of the present invention optimizes the level of sodium and typically also chloride and potassium levels so as to maximize rehydration without compromising the sensory properties of the beverage.
- potassium, magnesium and chloride at especially advantageous levels, the rehydration effects are further improved without changing the palatability of the beverage.
- the combination of extracellular-favoring ions at a level of between about 40-78 mEq/L, the advantageous rehydration and sensory properties are promoted.
- the experimental formulation having 30 mEq/L of sodium remained the same. It was compared to different experimental formulations. In addition, blood and urine chemical analyses were conducted. The experimental formulation having 30 mEq/L of sodium had improved rehydration properties; urinary fluid loss was reduced, the percentage of fluid retained increased, and overall body weight change was greater.
- the second of these experiments compared the 30 mEq/L of sodium formulation with a 25 mEq/L of sodium formulation, but this time the combined levels of sodium and chloride were more closely matched in the two formulations. (See the formulation table in example 7). No differences in fluid retention were found between these formulations. However, the 25 mEq/L of sodium formulation received initial negative sensory ratings versus the formulation having 30 mEq/L of sodium, but less chloride.
- the beverage of the present invention typically includes from about 4% to about 10%, preferably from about 5.5% to about 6.5%, more preferably about 6% by weight of a carbohydrate source.
- Carbohydrate sources include but are not limited to, sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate.
- a mixture of a minimum of three of these is formed, with the amount of fructose being less than the total amount of glucose from all carbohydrate sources.
- a preferred composition of carbohydrates comprises from about 1% to about 5% sucrose, from about 1% to about 2.5% glucose and from about 0.8% to about 1.8% fructose to produce a total of 6% carbohydrate, and more preferably from about 2 to about 4% sucrose, from about 1.4 to about 2% glucose, and about 1.1 to about 1.5% fructose, to produce a total of 6% carbohydrates.
- the sodium content of the beverage of the present invention comprises at least about 30 mEq/L, preferably from about 30 to about 100 mEq/L of beverage, more preferably from about 30 to about 60 mEq/L of beverage, even more preferably from about 33 to about 40 mEq/L.
- This sodium concentration indicates the total amount of sodium present in the beverage, including sodium contained in the carbohydrate source, flavoring agent (to the extent known), and clouding agent.
- maltodextrin as a carbohydrate source may contain sodium.
- these sources alone cannot raise the sodium levels of the beverage to the necessary levels, and as such additional sodium must be added from another sodium ion source.
- any source of sodium known to be useful to those skilled in the art can be used in the present invention.
- useful sodium sources include, but are not limited to, sodium chloride, sodium citrate, sodium bicarbonate, sodium lactate, sodium pyruvate, sodium acetate and mixtures thereof.
- a mixture of sodium chloride and sodium citrate being preferred, and a mixture of from about 10 to about 50 mEq/L, preferably from about 10 to about 30 mEq/L, and more preferably from about 10 to about 20 mEq/L of sodium from sodium chloride and from about 10 to about 50 mEq/L, preferably from about 10 to about 30 mEq/L, and more preferably from about 10 to about 20 mEq/L of sodium from sodium citrate.
- the beverage of the present invention also includes chloride.
- the chloride ion can come from various sources known to those skilled in the art. Examples of chloride sources include, but are not limited to, sodium chloride, potassium chloride, magnesium chloride and mixtures thereof.
- the concentration of chloride is at least about 10 mEq/L, preferably from about 10 to about 20 mEq/L, more preferably from about 11 to about 18 mEq/L of chloride from sodium chloride.
- the beverage of the present invention also preferably includes a combination of extracellular favoring ions, where the sum of these ions is from about 40 to about 78 mEq/L. This range can even be from about 42 to about 70 mEq/L or from about 46 to about 60 mEq/L.
- Sodium and chloride ions are some of the ions which favor the filling of the extracellular fluid compartment.
- the beverage of the present invention also includes potassium.
- the potassium ion source can come from many sources known to those skilled in the art as being useful in the present invention. Examples of potassium sources useful herein include, but are not limited to, potassium monophosphate, potassium diphosphate, potassium chloride, and mixtures thereof, with potassium monophosphate being preferred.
- the potassium content is at least 8 mEq/L, preferably from about 8 to about 20, and more preferably at from about 10 to about 19 mEq/L.
- the beverage of the present invention further preferably includes magnesium.
- the magnesium ion can also come from many sources known to those skilled in the art. Examples of magnesium sources include, but are not limited to, magnesium oxide, magnesium acetate, magnesium chloride, magnesium carbonate, magnesium diphosphate, magnesium triphosphate, magnesium in the form of an amino acid and mixtures thereof, with magnesium oxide being preferred.
- the concentration of magnesium is at a level of at least 0.1 mEq/L, preferably from about 0.5 to about 6 mEq/L, more preferably from 1 to 3 mEq/L.
- calcium preferably is present in the beverage of the present invention.
- the calcium ion may come from a variety of sources known to those skilled in the art. Examples include but are not limited to, calcium lactate, calcium carbonate, calcium chloride, calcium phosphate salts, calcium citrate and mixtures thereof, with calcium lactate being preferred. Calcium is present at a concentration of at least 0.1 mEq/L, preferably from about 0.5 to about 6 mEq/L, more preferably from 1 to 3 mEq/L.
- a flavoring agent may be used in the beverage of the present invention.
- the flavoring agent of the beverage of the present invention also impacts the overall acceptance of the beverage. In order to achieve this overall acceptance, the strength of the flavor cannot be too intense.
- the flavor intensity of the beverage will depend upon the amount and type of the particular flavoring agent used.
- the same flavor from different suppliers may have differing intensity. Thus, it is difficult to quantify the level of flavoring agent necessary for the present invention.
- a flavoring agent at a concentration of from about 0% to about 0.400% by weight is useful in the present invention.
- flavoring agents themselves may contain gum arabic, ester gum, starches such as, dextrins, “modified food starch”, propylene glycol or alcohol. These additional components may acts as carriers or stabilizers.
- any flavoring agent which satisfies the above criteria and is known to be useful to those skilled in the art can be used in the present invention.
- particularly useful flavoring agents include but are not limited to, lemon-lime, orange, and fruit punch.
- the lemon-lime flavoring agent can be at a concentration in the range of from about 0.050% to about 0.200%, preferably from about 0.080 to about 0.150%, and more preferably from about 0.090 to about 0.120% by weight.
- the beverage of the present invention is formulated to have an osmolality, when initially formulated, in the range of from about 220 to about 350 mOsm/Kg of beverage, and is preferably in the range of from about 250 to about 330, more preferably from about 260 to about 320 mOsm/Kg of beverage.
- the beverage of the present invention are isotonic.
- the scientific and strict definition of the term isotonic is a solution that has the same or nearly the same osmotic pressure as another solution, typically human blood.
- the beverages of the present invention can be isotonic when prepared, even with regards to the strict scientific meaning of the term, the term isotonic as presently used is not meant to be so narrowly defined. With respect to the present specification, isotonic is meant to refer to the fact that the beverages of the present invention are sports type beverages which contain a certain amount of carbohydrates and electrolytes.
- the beverage of the present invention may also include a clouding agent at a concentration range of from about 0 to about 100 ppm of clouding agent.
- clouding agents include, but are not limited to, ester gum, SAIB, starch components and mixtures thereof, with ester gum as the preferred clouding agent at a concentration range of from about 10 to about 50 ppm and more preferably from about 15 to about 35 ppm.
- the beverage of the present invention may further include citric acid at a concentration range of from about 0.24% to about 0.45% by weight.
- Citric acid lowers the pH in order to insure it is a high acid beverage which may be pasteurized under conditions less harsh than required for low acid beverages.
- Beverages of the present invention preferably have a pH of from about 2.5 to about 4.5, preferably from about 2.75 to about 4.25, more preferably from about 2.9 to about 4.0.
- citric acid adds tartness to the beverage.
- the present invention also relates to a beverage concentrate used to prepare the beverage already described herein.
- beverage concentrate refers to a concentrate that is either in liquid or gel form or in essentially dry mixture form.
- the essentially dry mixture is typically in the form of a powder, although it may also be in the form of a single-serving tablet, or any other convenient form.
- the concentrate is formulated to provide a final and complete beverage as already described herein when constituted or diluted with water or other liquid.
- a preferred beverage concentrate of the present invention capable of producing the preferred beverage below when constituted or diluted with water comprises:
- a formulation designated as G30 was evaluated for sensory properties against four other formulations containing the typical ingredients found in commercial sports drinks.
- the G30 formulation consisted of a 6% carbohydrate solution in water, 30 mEq/L of sodium, 3 mEq/L potassium, about 10 mEq/L of chloride, 25 ppm of a clouding agent and 0.103% by weight of a flavoring agent.
- the carbohydrate solution was prepared using a mixture 3% sucrose, 1.7% glucose, and 1.3% fructose.
- both sodium chloride and sodium citrate were used to provide the sodium ions.
- the comparison formulations contained the exact ingredients except that the sodium levels of these beverages were modified so that the first formulation, designated as G0, contained 0 mEq/L of sodium, the second formulation, designated as G18, contained 18 mEq/L of sodium, the third formulation, designated as G40, contained 40 mEq/L of sodium, and the fourth formulation, designated as G60, contained 60 mEq/L of sodium.
- Post-exercise urine volumes indicated an inverse association between sodium level and urine volume produced, which is indicated in FIG. 4 .
- Urine volumes were directionally less as sodium level increased from 0 to 60 mEq/L and was least for G60 at 15 minutes post-exercise compared to other beverages. Analysis of the trend in the data (e.g. slope) for post-exercise urine excretion was significantly different from 0. That is, there is 95% confidence that the downward trend in the data was not due to chance alone. Overall, exercise urine volumes and total urine volumes were similar among beverages.
- Subjects were fed a standard diet for dinner (the evening prior to testing), breakfast, and lunch.
- the total caloric intake was 2440 calories and 2592 mg of sodium.
- Meals were accompanied with 500 ml of water.
- the average total urine losses were 0.546, 0.430, 0.322 and 0.287 liters for 0, 18, 30, 60 mEq/l beverages, respectively as shown in FIG. 9 .
- Both 0 and 18 were significantly (p ⁇ 0.05) different from 30 and 60.
- the 30 and 60 beverages did not differ from each other. Differences in urine loss did not become apparent until 1 hour after the end of the drinking period.
- By 2 hours after the drinking ended (3.5 hrs post-exercise), differences among 0, 18, and the other beverages were greatest.
- Average fluid intakes ranged from 1.8 to 1.95 liters, but were not different across formulations. Two hours after rehydration with a volume equivalent to 100% of total sweat lost, the total percent of fluid retained was 69%, 75%, 82%, and 83% for 0, 18, 30, and 60 mEq/L, respectively as shown in FIG. 10 .
- the G30 beverage scored directionally higher than all other beverages. Sensory evaluation indicated that the 30 mEq/L was liked equally well as G18, but was directionally favored over all other beverages for characteristics of sweetness, flavor, tartness, saltiness, and overall acceptance. For the G18 and G30 beverages, ratings of perceived saltiness were very close to ideal ratings of saltiness throughout the drinking period. In general, hedonic scores declined with increasing time (30 to 90 min) during the rehydration period.
- the G30 formulation performed directionally better than G18 for a number of hedonic ratings and provided a greater fluid retention response during rehydration than other beverages.
- the highest sodium level in the G60 formulation provided no further benefit beyond the G30 formulation in terms of attenuating fluid loss in the form of urine.
- G30 provides the following advantages over G0, G18, G60: (1) directionally higher hedonic ratings in athletes undergoing exercise in the heat and (2) attenuated urinary fluid losses after rehydration with a volume equivalent to 100% of sweat loss.
- Example 3 The same formulations as Example 3 were used except that a G5 formulation was included and the G0 and G60 formulations were eliminated.
- the G5 formulation was identical to the other formulations except that the sodium level was adjusted to 5 mEq/L.
- This testing compared the formulations to determine how rapidly fluid is lost and how much is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss.
- Certain biochemical parameters were measured to determine physiology related changes. The parameters measured were blood related changes in [Na+], [K+], [Ca++], osmolality, Hb, Hct, ⁇ PV, glucose, pH, and urine related changes in volume, osmolality, [Na+], [K+], SEC, FWC, creatinine, and GFR. This was also conducted in a double-blind experimental design with formulations counterbalanced among the study group.
- each subject was required to ingest fluid adequate to establish euhydration. This included a minimum intake of 500-ml of water the evening prior to the test as well as 1-liter of water the day of the test. Pre-experiment hydration was further assured by checking the conductivity of the pre-experiment urine sample prior to the experiment. Subjects whose first experimental urine sample had a conductivity that exceeded 21 mS-cm were dismissed from the experiment and required to reschedule. An indwelling venous catheter was inserted into a forearm vein for repeated blood sampling throughout the experiment.
- a previously determined workload was used to set an exercise intensity equivalent to 70-75% of maximal heart rate as determined by earlier maximal treadmill tests.
- Heart rate was monitored every 10 minutes using a telemetered HR monitor (Polar) and to assure sufficient intensity of exercise.
- a whole blood clinical analyzer (Instrumentation Laboratories, Synthesis IL1735) was used to measure changes in blood sodium, potassium, ionized calcium, glucose, hematocrit, hemoglobin, and pH value. Approximately 1-ml of whole venous blood was collected in a 3-ml heparinized arterial blood-gas syringe (Marquest, GASLYTE) and was used specifically for the clinical analyzer. The arterial blood gas syringe was chosen to avoid sample clotting and to ease presentation of the sample to the clinical analyzer.
- Plasma volume changes were estimated from a published equation (Dill/Costill) relating hematocrit and hemoglobin changes. Plasma osmolality was measured by freezing point depression method on a FISKE 2400 multiple sample osmometer.
- Urine volume was determined by weight, and urine conductivity was immediately determined using a conductivity meter (WTW LF 340, model 19706-20). Urine was then aliquoted and stored at ⁇ 20° F. until later analysis for [Na+], [K+], osmolality and creatinine. Urine [Na+] and [K+] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Urine creatinine was determined on the spectrophotometer utilizing a Sigma Creatinine Kit (Sigma Diagnostics, No. 555). Urine osmolality was determined by the freezing point depression method on an osmometer after thawing frozen samples to room temperature.
- FIG. 12 shows cumulative urine loss among the three formulations across recovery time. Effect sizes estimates for total urine loss were 0.39 (G5 vs. G18), 0.97 (G5 vs. G30), and 0.70 (G18 vs. G30).
- FIG. 13 shows fluid retention during recovery for the three formulations tested.
- Absolute change in bodyweight from baseline weight was significantly greater for G5 compared to G18 and G30 (P ⁇ 0.05).
- Bodyweight losses, corrected for any fluid gains during recovery, were 0.93, 0.80 and 0.74 kg, respectively.
- ANOVA analysis revealed a main effect of sodium (P ⁇ 0.001) and time (P ⁇ 0.0001), with an interaction between sodium and time (P ⁇ 0.0001) on individual urine volumes excreted. Independent of time (summed across time points), average urine volumes were 79.8, 68.0, and 49.7 g for G5, G18, and G30, respectively.
- Urine conductivity varied by sodium level and time, with an interaction between sodium and time (P ⁇ 0.01). Independent of time (summed over all time points), average urine conductivity was 12.3, 12.8 and 14.8 mS-cm for G5, G18, and G30, respectively. Conductivity was significantly higher for G30 compared to G5 and G18.
- Urine sodium and potassium concentrations were not different between sodium levels, but did differ by time. Urine sodium and potassium excretion, corrected for volume, did not differ between formulations, but did differ over recovery time. When summed across formulations, average [Na+] excreted was significantly higher at 0 minutes recovery (7 mEq), dropped to 4.4 mEq at 30 minutes into the drinking period, and remained between 2.2-2.4 mEq from 90 through 210 minutes of recovery.
- the G30 formulation was compared to water (W), a commercial product POWERade® (P), and a G18 formulation identical to the G30 formulation except that the sodium level was adjusted to 18 mEq/L.
- Specific electrolyte formulations are shown below. [Na + ], [K + ], [Cl ⁇ ], Osm Code mEq/L* mEq/L* mEq/L (mOsm/kg)* W 0 0 0 0 P 5 2 5.8 428 G18 18 3 9 320 G30 30 3 9 334 *Indicates measured analytical values
- Beverage temperature was maintained between 41-45° F. and served at that temperature and the beverages were blinded to the subjects as well as the experimenters.
- Workloads were set for the cross-trainer, stationary bike and treadmill sufficient to produce intensities between 70-75% of their measured maximum heart rates (determined from annual stress tests).
- body weights were obtained prior to and following the orientation exercise session in order to predict sweat rates.
- Subjects were provided with standardized meals to ensure consistent sodium intake ( ⁇ 2900 mg) prior to each of the two trials. Meals included dinner (evening prior to experiment), and breakfast and lunch on the day of testing. Subjects were given the option of eating all or a portion of the food provided but were instructed to record which foods and amount left uneaten. These items were then withheld from the food bags for the next trial. In addition, they were given bottled water to drink the evening before (500 ml) and during the testing day (1000 ml) to ensure adequate hydration. Subjects were asked to refrain from caffeine and alcohol use for 24-hours prior to the experiment. Most subjects ate all the food provided to them, however, intakes ranged from 2,109-2,278 kcal and 2,849-2,960 mg sodium. None of the subjects ate food other than what was provided.
- the exercise session consisted of 30-minutes each on the cross trainer, stationary bike and treadmill at 75-80% maximum heart rate for a total of 90 minutes. Heart rates were taken at 15-minute intervals to ensure subjects maintained adequate intensity. Subjects refrained from drinking during the entire exercise period in an effort to produce 2-2.5% dehydration.
- Urine volume was determined by weight and urine specific gravity was measured (A 300 Clinical Refractometer). Urine was the transferred into 4-ml cryovials for further analysis. Urine [Na+] and [K+] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- SPSS version 10.0 was used to analyze the data. ANOVA using a general linear model was used to determine differences among mean values. Data is reported as the mean+the standard deviation.
- Total cumulative fluid output was significantly different among formulations. W resulted in significantly more urine loss than the other three formulations (0.726 ⁇ 0.225 L). P and G18 were not significantly different from each other at 0.496 ⁇ 0.184 L and 0.428 ⁇ 0.196 L, respectively. G30 was different from W and P, but not from G18 (0.367 ⁇ 0.263 L). Urine output at each data collection point was not significantly different among formulations for time points 60, 90 or 120. However, at time point 180, W resulted in greater urine loss compared to the other three formulations.
- Fluid retention or the amount of fluid consumed that was not excreted as urine, was different among formulations. This was calculated in both relative terms (ml/kg) and as a percentage calculated as (fluid in ⁇ fluid out)/fluid in. Relative fluid retention for W was 17.07 ⁇ 5.22 ml/kg, significantly less than the other formulations. P, G18, and G30 were 20.43 ⁇ 6.50 ml/kg, 19.48 ⁇ 4.83 ml/kg, and 21.26 ⁇ 4.83 ml/kg, respectively.
- Percent fluid retained was different among formulations (62.94 ⁇ 9.05% for W, 74.25 ⁇ 11.15% for P, 76.52 ⁇ 10.32% for G18, and 81.45 ⁇ 10.34% for G30) with W significantly less than P, G18 and G30 at minutes 180 and 240. Additionally, fluid retention was significantly greater in the G30 trial compared to W and P at the end of recovery (minute 240 ). G18 was not different from P or G30. See FIG. 15 .
- Average urine osmolality was different among formulations. W resulted in the lowest urine osmolality (348.33 mOsm) and was not different from P (400.66 mOsm) but was significantly different from G18 (431.74 mOsm) and G30 (500.42 mOsm). G18 was not different from P or G30. P was significantly less than G30. See FIG. 17 .
- the other trials did not differ from each other. See FIG. 19 .
- Urine protein was measured via reagent strips (Uristix) to determine whether or not dehydration has an effect on protein excretion.
- TABLE III is a chart indicating the frequency and amount of protein detected in the urine at each time point for each of the four formulations.
- Protein Treatment Time (mg/dl) Water POWERade ® G18 G30 Pre- Negative 16 17 17 17 Exercise Post- Negative 16 13 10 13 Exercise Trace 0 3 5 3 60 Negative 9 8 10 8 Trace 6 5 5 5 5 5 30-100+ 1 2 0 3 100+ 1 1 90 Negative 10 12 11 9 Trace 4 4 0 5 30-100 1 1 0 2 100+ 1 120 Negative 16 16 11 13 Trace 0 1 1 4 30-100 1 180 Negative 15 16 10 13 Trace 0 0 1 2 240 Negative 15 16 10 11 Trace 0 0 1 5
- G30 and G18 were few and rather random. Although the G30 formula was initially less acceptable than the G18 for overall acceptance, flavor, sweetness, and tartness at the initial sensory read (32 minutes into recovery time), the differences between these two beverages decreased rapidly with time. While G18 generally had a numerical score advantage at 42, 62, and 82 minutes in these categories, the advantage was statistically insignificant. The G30 formula scored lower than the G18 for tartness at 62 minutes.
- the G18 formula was rated less salty than ideal at all evaluation times. G18 was rated more sour than ideal at 32, 42, and 82 minutes. There were no differences in perceived saltiness between G18 and G30. Compared to G18, G30 was less sour at 42 and 62 minutes, sweeter at 82 minutes, and less off at 62 minutes. The G30 formula was rated less salty than ideal at all evaluation times.
- subjects were divided into two groups to assess the effect of controlling pre-experiment diet (over 24 hours) on physiology measures. As in the previous phase, subjects were fed a standard diet for dinner the evening prior to testing, and for breakfast and lunch the day of the test.
- the total caloric intake was 2200 calories and approximately 2400 mg of sodium. Subjects who weighed over 150 pounds had slightly higher (+200) calories and sodium intake (+100 mg). All subjects were given a written copy of the dietary and exercise guidelines, and subjects were instructed not to substitute foods, but additional liquids (within guidelines) were permitted until 3 hours prior to the experiment.
- Subjects were then weighed for a baseline bodyweight, and a sensory (GI Symptoms, Energy levels) questionnaire was completed. Subjects cycled in a warm environment (80° F., 40% RH) for 1.5 hours to elicit an exercise-induced dehydration near two percent of initial bodyweight. A previously determined workload was used to set an exercise intensity equivalent to 70-75% of maximal heart rate as determined by earlier maximal treadmill tests. Heart rate was monitored every 10 minutes using a telemetered HR monitor (Polar) and to assure sufficient intensity of exercise.
- GI Symptoms, Energy levels GI Symptoms, Energy levels
- Beverages were mixed prior to the experiment using 1-liter and 2-liter volumetric flasks and beverage grade water that did not contribute any electrolytes to the beverage. Beverages were served chilled from the refrigerator (approximately 40° F.), although the last two beverages were likely to be closer to ambient temperature by the time they were served to the subjects. Beverages were distributed according to a rehydration scheme that involved dosing the volume according to specific times. At 30 minutes into recovery, subjects received their first of six cups of beverage, followed by another cup every ten minutes. Total beverage volume was equivalent to total sweat loss, and was portioned such that subjects received 50% of the total volume in the first 20 minutes and the remaining 50% in 12.5% portions every ten minutes for the remaining 40 minutes. All fluid was consumed within an hour.
- Urine volume was determined by weight, and urine conductivity was immediately determined using a conductivity meter (WTW LF 340, model 19706-20). Urine was then aliquoted and stored at ⁇ 20° F. until later analysis for [Na + ] and [K + ]. Urine [Na + ] and [K + ] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed.
- flame photometry IL943 Automatic Flame Photometer
- the percent of ingested fluid retained differed significantly. G25 resulted in significantly more fluid retention (79.6%) compared to G18 (73.5%) and G30 (75.1%). When corrected for bodyweight (ml/kg) or examined in absolute terms (kilograms), the significant effect disappears. Contrary to results from Project FR-1 phases 2, 3, and 4, G30 did not differentiate from G18 in this study.
- Absolute change in body weight from baseline to end of recovery was ⁇ 0.72, ⁇ 0.62, and ⁇ 0.71 kilograms for G18, G25, and G30, respectively. Expressed as a percentage of initial weight, these values were ⁇ 0.98%, ⁇ 0.84%, and ⁇ 0.96% for G18, G25, and G30, respectively. Both absolute and percentage variables were significantly different for G25 versus G18 and G30 when subject variability was controlled for in the statistical model. G18 did not differ from G30.
- SEC Specific Electrical Conductivity
- Subjects were provided with standardized meals to ensure consistent sodium intake ( ⁇ 2700 mg) prior to each of the two trials. Meals included dinner (evening prior to testing), and breakfast and lunch on the day of testing. Subjects were given the option of eating all or a portion of the food provided but were instructed to record which foods and amount left uneaten. These items were then withheld from the food bags for the next trial. In addition, they were given bottled water to drink the evening before and during the testing day to ensure adequate hydration. Subjects were asked to refrain from caffeine and alcohol use for 24-hours prior to the experiment.
- GI ratings After completing a pre-exercise survey (GI ratings), they began exercise. The exercise session consisted of alternate cycling/running at 15-minute intervals at 75-80% maximum heart rate for a total of 60 minutes. Heart rates were taken at 15-minute intervals to ensure subjects maintained adequate intensity. Subjects refrained from drinking during the entire exercise period in an effort to produce 1.5-2% dehydration.
- Drink #5 (12.5%) was given at 70 minutes
- drink #6 (12.5%) their final drink
- a sensory questionnaire was given at 80 minutes.
- 120, 180 and 240 minutes GI scales were given and urine samples were collected. After the final urine sample, subjects were taken back upstairs for a final nude body weight. They were given meals for the next trial (if necessary) and were released.
- Urine volume was determined by weight and urine specific gravity was measured (A 300 Clinical Refractometer). Urine was the transferred into 4-ml cryovials for further analysis. Urine [Na + ] and [K + ] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- Fluid retention or the amount of fluid consumed that was not excreted as urine, was not different between treatments. This was calculated in both relative terms (ml/kg) and as a percentage calculated as (fluid in ⁇ fluid out)/fluid in. Relative fluid retention was 13.11 ⁇ 3.04 ml/kg and 13.32 ⁇ 3.52 ml/kg for G25 and G30, respectively. Percent fluid retained was also the same for both treatments (74.29 ⁇ 9.76% for G25 and 74.20 ⁇ 10.14% for G30). Predictably, percent fluid retention across time was also the same for each treatment (see below).
- Subjects were asked to rate a number of psychophysiological characteristics on several different scales (e.g. categorical, 100 pt) throughout the experiment period to examine any sensory and perceptual changes associated with the treatments.
- a GI distress and a Sensory (taste) form were used periodically throughout the test to examine subjects' perceptions. There were no important differences between the two beverages on ratings of perceived physiological and psychological well-being. Differences in beverage acceptance are as follows.
- the G30 scored significantly higher than G25 in acceptance and liking of sweetness, tartness, flavor, and saltiness, and had a large effect size (0.7) advantage in aftertaste at 32 minutes. See FIG. 24 for overall acceptance. At 42 minutes, G30 was significantly more acceptable than G25 for aftertaste, and had a medium effects size advantage in tartness.
- G25 was more tart than G30 at all times. See FIG. 25 . G25 was significantly more salty than G30 at 32 minutes and was not different at all other time points. G25 perception of saltiness was initially rated as ideal but became decreasingly less than ideal at all other time points. G30 was less than ideal saltiness at all time points. See FIG. 26 .
- Subjects were provided with standardized meals to ensure consistent sodium intake ( ⁇ 3000 mg) prior to each of the experimental trials. Meals included dinner (evening prior to experiment), and breakfast and lunch on the day of testing. Subjects were given the option of eating all or a portion of the food provided. They were also instructed to record which foods were left uneaten. These items were then withheld from the food bags for the next trial. In addition, they were given bottled water to drink the evening before (500 ml) and during the testing day (1000 ml) to ensure adequate hydration. Subjects were asked to refrain from caffeine and alcohol use for 24-hours prior to the experiment.
- the exercise session consisted of 30-minutes each on the cross trainer, stationary bike and treadmill at 70-75% maximum heart rate for a total of 90 minutes. Heart rates were taken at 15-minute intervals to ensure subjects maintained adequate intensity. Subjects refrained from drinking during the entire exercise period in an effort to produce 2-2.5% dehydration.
- the fifth aliquot (12.5%) was given at 70 minutes, and the sixth and final aliquot (12.5%) and a sensory questionnaire was given at 80 minutes.
- GI scales were given and urine samples were collected. After the final urine sample, subjects were weighed for a final nude body weight.
- Urine volume was determined by weight and urine specific gravity was measured (A 300 Clinical Refractometer). Urine was then transferred into 4-ml cryovials for subsequent analysis of sodium and potassium concentrations using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- SPSS version 11.0 was used to analyze the data. ANOVA using a general linear model was used to determine differences between mean values. Data is reported as the mean+the standard deviation.
- the average urine potassium concentration was significantly higher for the K20 trial (58.44 ⁇ 32.59 mEq/L) compared to P (42.24 ⁇ 40.19 mEq/L). G18 (47.26 ⁇ 37.05) and K10 (51.99 ⁇ 39.33) were not different from any of the treatments.
- Formula K20 and Powerade scored significantly higher for saltiness acceptance than G18 at 32 minutes. This was the only case where a significant difference was found across products at any time. P showed a significant decline in overall acceptance with time. While P initially, had the numerically highest overall acceptance score, it was directionally the least acceptable overall. P also had directional decreases over time for all acceptance scales except aftertaste. Formula G18 had relatively low initial (32 minute) scores for liking of aftertaste, saltiness, and tartness. These scores rebounded into a more typical range at later times.
- the exercise induced 2.5-3% dehydration in the subjects.
- Final body weight as a percentage of initial body weight did not differ between treatments. Subjects returned to 98.96 ⁇ 0.21% of initial body weight following the G30 trial, 98.96 ⁇ 0.29% following K5, and 98.90 ⁇ 0.37% and 98.95 ⁇ 0.31% for K10 and K20, respectively.
- fluid retention did not differ either: 16.59 ⁇ 5.09 ml/kg, 17.29 ⁇ 5.04 ml/kg, 16.69 ⁇ 6.06 ml/kg, and 16.66 ⁇ 5.31 ml/kg for G30, K5, K10 and K20, respectively.
- the average urine potassium concentration was significantly higher for the K20 trial (56.59 ⁇ 33.43 mEq/L) compared to G30 (49.79 ⁇ 32.87 mEq/L), K5 (47.24 ⁇ 36.11) and K10 (46.87 ⁇ 28.96). The latter three were not different from each other. See FIG. 34 .
Abstract
The present invention discloses novel compositions and methods. These compositions and methods may be used to attenuate or reverse the effects of dehydration or other adverse effects of exercise, heat or other activity which causes bodily fluid loss. The novel compositions comprise carbohydrates, electrolytes and water along with flavoring agents.
Description
- The present invention relates to an improved rehydration beverage which provides for fluid, carbohydrate, and electrolyte replacement. The beverage comprised of a novel mineral blend enhances rehydration by providing superior voluntary fluid consumption and fluid retention. Non-fluid components of the beverage composition can be provided in forms other than beverages.
- There are currently available a number of beverages, and concentrated beverage compositions (including liquid, powdered or tablet concentrates) to be prepared by the consumer into a drinkable form, which are used for rehydration of fluids lost during exercise or vigorous activity. These rehydration beverages (also known as “sports drinks”), both in the ready-to-drink form and as prepared by the user, may be consumed before, during and after exercise. While these sports drinks rehydrate the body better than plain water, there remains a need for a superior rehydration beverage that addresses many aspects of rehydration such as voluntary fluid consumption and fluid retention.
- Rehydration can be accomplished in a number of different ways. In the most basic sense, water replaces some of the fluids lost through sweat and helps maintain body temperature and important cardiovascular functions. Sports drinks have been developed which replace the fluids and electrolytes lost through sweat. This is an improvement over plain water not only because these drinks replace some of the minerals lost in sweat but also because carbohydrates are provided as a source of added energy. However, rehydration would be improved if the beverage also improved fluid retention, reduced urinary fluid loss, stimulated increased voluntary consumption, possessed superior sensory qualities, and supported the physiological response to continue drinking.
- Conventional sports drinks typically contain water, small amounts of minerals/electrolytes and an energy source in the form of carbohydrate compounds. The scientific rationale for inclusion of common electrolytes such as sodium and potassium rested on the intuitive notion that it would be logical to replace some of the electrolytes lost in sweat. Stimulating voluntary fluid intake and promoting fluid retention were either secondary considerations or worse, not considered at all.
- In addition, the mineral content of sports drinks varies widely. For instance some beverages can contain 5 mEq/L of sodium while others can contain over four times that amount. Similarly, there are wide differences in the content and amounts of other minerals, such as potassium, magnesium and chloride.
- Some research has indicated that sodium content in fluid replacement beverages has a marked effect on fluid retention after exercise. Basically this research shows that when subjects ingest standard volumes of solutions containing sodium in the range of 40-100 milliequivalents per liter (mEq/L), rehydration is improved by increasing fluid retention and maintaining positive fluid balance. However, the palatability of a fluid replacement beverage containing an increased amount of sodium was not considered. Nadel E., Mack G., and Takamata, A., Thermoregulation, Exercise, and Thirst: Interrelationships in Humans. In Perspectives in Exercise Science and Sports Medicine: Exercise, Heat, and Thermoregulation, vol. 6, pp. 225-251, (Gisolfi, Lamb and Nadel eds., Indianapolis Ind., Brown and Benchmark, 1993); Maughan, R. J. and J. B. Leiper, Sodium Intake and Post-Exercise Rehydration in Man, Eur. J. Appl. Physiol. 71:311-319, (1995); Shirreffs, S. M. and R. J. Maughan, Volume Repletion after Exercise Induced Volume Depletion in Humans: Replacement of Water and Sodium Losses, Am. J. Physiol. 43:F868-F875, (1998). These articles are hereby incorporated by reference.
- Fluid replacement after significant dehydration is driven by various physiological changes. The two major physiological drivers that encourage voluntary drinking are plasma osmolality and plasma volume. During exercise, the loss of fluid through sweat causes plasma volume to drop and plasma osmolality to increase. These physiological changes cause a thirst response which drives voluntary fluid consumption. Scientific studies have shown that sodium also plays an important role in regulating plasma volume and osmolality. Ingesting beverages containing sodium helps increase the rate at which plasma volume and osmolality return to normal. However, ingesting too high a level of sodium causes rapid restoration of plasma volume, which reduces the drinking response and prevents adequate rehydration. In addition, the sensory properties of a beverage containing too high a level of sodium are unfavorable, and would further reduce the drive to drink. Wemple, Richard D., Morocco, Tamara S., Mack, Gary W., Influence of Sodium Replacement on Fluid Ingestion Following Exercised-Induced Dehydration, Int'l J. Sport Nutrition & Exerc. Metabolism 7:104-116 (1997). This article is hereby incorporated by reference.
- It is further believed that other electrolytes and minerals play an important role in rehydration by possibly affecting fluid replacement and fluid retention. In response to fluid loss during dehydration, water is distributed between fluid compartments so that both the extracellular and intracellular compartments share the water deficit. Sodium, potassium, magnesium, calcium and chloride are some of the more important electrolytes/minerals involved in filling these body fluid compartments, particularly sodium, chloride, potassium and magnesium. Beverages providing sodium and chloride encourage the filling of the extracellular compartment, while beverages providing potassium, magnesium, and calcium favor the filling of the intracellular compartment. Properly balancing the sodium, potassium, magnesium, calcium and chloride levels will further improve the rehydration properties of the beverage.
- These electrolyte ions assist in filling these body fluid compartments more rapidly and help to retain the fluid instead of it being excreted as urine. Since both sodium and chloride ions favor the filling of the extracellular compartments, substitution of one with the other may not affect the overall result. The same may be true for potassium and magnesium in regards to intracellular hydration.
- U.S. Pat. No. 4,981,687, issued to Fregly et al., incorporated herein, discloses a beverage comprising water, sugar, and electrolytes, an improvement wherein said beverage further comprises glycerol, pyruvate and/or caffeine. The sugar contained in the beverage claimed in this patent can be glucose, sucrose or other appropriate sugar compound, with glucose at a concentration of from about 2% to about 8% being specifically disclosed and glucose at a concentration of about 4% being preferred. Examples of electrolytes disclosed in this patent include 15-30 mEq/L sodium, 1-5 mEq/L potassium, 2-8 mEq/L phosphate, bicarbonate, sulfate, chloride, calcium, and magnesium.
- The beverages as disclosed above are said to ameliorate the adverse physiological effects which can result from physical exertion and heat exposure. The present invention also seeks to address the adverse physiological effects of physical exertion, but without including stimulants or other chemical compounds which may have known acute effects (e.g. glycerol and pyruvate promoting gastrointestinal distress) and unknown long-term effects.
- Therefore, a need exists for a rehydration beverage that supplies necessary electrolytes and energy sources, exhibits organoleptic properties which are substantially equivalent or superior to those of typical sports drinks, and provides superior rehydration by addressing several mechanisms affecting rehydration, such as fluid retention and voluntary fluid consumption. Furthermore, a need exists for a method that ameliorates the effects of dehydration, enhances rehydration, and addresses mechanisms such as fluid retention, and urinary loss.
- The present invention fulfills these needs. The beverages of the present invention enhance rehydration, supply necessary electrolytes and energy sources, exhibit organoleptic properties at least substantially equivalent to other sports drinks, improve fluid retention and voluntary fluid consumption. The method of the present invention also addresses the above concerns through the administration of the composition of the present invention. The composition may be administered orally. And the composition can take many forms including but not limited to, liquid, gel, dry powder, tablet or capsule. Concentrated forms of the composition such as a powder can be can be added to water and/or other liquids which can include electrolytes and/or carbohydrates, including even sports drinks such as Gatorade® in order to provide beverages of the present invention.
- Dehydration is reduced and fluid retention is improved by abating urinary loss when one embodiment of the present invention is taken during exercise, as illustrated in Example 2 hereof. Examples 3 through 7, further show that the present invention reduces the effects of dehydration, improves fluid retention, reduces urinary fluid loss, and possess superior sensory properties which should improve voluntary fluid consumption when taken after activity-induced fluid loss. It is further believed that similar results should be obtained when the beverage of the present invention is taken before activity-induced fluid loss.
- The present invention relates to a beverage composition for oral consumption comprising: from about 4% to about 10% by weight of carbohydrates; at least about 30 mEq/L of beverage of sodium; at least about 7 mEq/L of beverage of potassium; from about 10 to about 20 mEq/L of beverage of chloride; from about 0% to about 0.4% of a flavoring agent, when present; from about 0 to about 100 parts per million (ppm) of a clouding agent, when present; from about 0.24% to about 0.38% by weight citric acid, when present; and typically balance water. Furthermore, the extracellular-favoring ions (especially sodium and chloride) may be present in combination at levels of from about 40 to about 78 mEq/L of beverage. These quantities are based on the total content of a finally prepared, complete beverage. It will be appreciated that the complete beverage can be fully formulated as noted above, or it can be made up by providing portions or all of a component or components by added liquids, whether the liquid is water or water already containing components adequate to prepare the final beverage. In the latter case, the formulation added to the liquid has components so as to make up the finally prepared beverage when the formulation and the liquid are combined to form the complete beverage.
- The osmolality of the beverage is in the range of from about 250 to about 350 mOsm/Kg. The beverage also may include from about 1 to about 6 mEq/L of calcium; and when present, from about 1 to about 6 mEq/L of magnesium.
- The present invention also relates to a beverage concentrate which is formulated to provide the beverage already described herein upon preparation by the consumer. The concentrate can take many forms including, but not limited to, gel, dry powder, tablet, capsule and liquid concentrates. The concentrates may be added to water and/or other liquids including water and carbohydrates and/or electrolytes, such as Gatorade®. Administration can be oral, intravenously, or by other suitable means.
- The advantages of the invention can be better understood by referring to the following drawings.
-
FIG. 1 shows the hedonic scores of various formulations with regard to overall acceptance and liking of saltiness when consumed during exercise. -
FIG. 2 illustrates the experimental procedure employed to determine ad-libitum drinking characteristics, sensory aspects, hydration characteristics, and voluntary fluid intake of various formulations. -
FIG. 3 shows the total fluid consumed for each of various formulations. -
FIG. 4 shows the trend in urine loss by graphing the urine volume for each formulation. -
FIG. 5 shows dehydration in terms of initial body weight of individuals to whom each formulation was administered. -
FIG. 6 illustrates the ratings of saltiness intensity during exercise and sedentary conditions and further shows the ideal perceived saltiness. -
FIG. 7 shows hedonic scores in terms of overall acceptance and liking of saltiness for the various formulations during exercise and sedentary conditions. -
FIG. 8 illustrates the experimental protocol used to determine how much fluid is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss. -
FIG. 9 shows cumulative urine loss over time during the recovery period for the various formulations. -
FIG. 10 shows fluid retention as a percentage of the volume ingested during recovery time for the various formulations. -
FIG. 11 illustrates the experimental protocol used to determine how much fluid is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss. -
FIG. 12 shows cumulative urine loss over time during the recovery period for various formulations. -
FIG. 13 shows fluid retention as a percentage of the volume ingested during recovery time for various formulations. -
FIG. 14 shows the plasma volume changes over time for various formulations. -
FIG. 15 shows fluid retention as a percentage of the volume ingested during recovery time for various formulations. -
FIG. 16 shows urine production over time during a recovery period for various formulations. -
FIG. 17 shows the urine osmolality over time-before exercise and during recovery-for various formulations. -
FIG. 18 shows urine sodium losses over time for various formulations. -
FIG. 19 shows urine potassium losses over time for various formulations. -
FIG. 20 shows urine specific gravity over time for various formulations. -
FIG. 21 illustrates the experimental protocol used to determine how much fluid is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss. -
FIG. 22 shows the percentage of fluid retained over time of the various formulations. -
FIG. 23 shows the cumulative urine loss in grams over time of the various formulations. -
FIG. 24 indicates the overall acceptance of the various formulations during the rehydration period preceding the measurement of fluid retention. -
FIG. 25 indicates the tartness scores of the various formulations during the rehydration period preceding the measurement of fluid retention. -
FIG. 26 indicates the perceived saltiness of the various formulations during the rehydration period preceding the measurement of fluid retention. -
FIG. 27 shows the percent fluid retained as a percentage of the volume ingested during recovery time for various formulations and historical range of fluid retention. -
FIG. 28 shows the urine osmolality over time-before exercise and during recovery-for various formulations. -
FIG. 29 shows the urine sodium concentration during the recovery period for various formulations. -
FIG. 30 shows the urine specific gravity over time-before and after exercise and during recovery-for various formulations. -
FIG. 31 shows the percent of total fluid retained at the end of the recovery period for various formulations. -
FIG. 32 shows the urine osmolality over time-before exercise and during recovery-for various formulations. -
FIG. 33 shows the urine specific gravity over time-before and after exercise and during recovery-for various formulations. -
FIG. 34 shows the urine potassium concentration during the recovery period for various formulations. -
FIG. 35 shows the overall acceptance ratings from the sensory evaluations for various formulations. - The beverage of the present invention provides superior rehydration by improving fluid retention and reducing urine fluid loss. Additionally, the beverage enhances rehydration by also improving voluntary fluid intake. Moreover, the organoleptic or sensory properties of the beverage are at least as favorable as other sports drinks.
- The beverage of the present invention optimizes the level of sodium and typically also chloride and potassium levels so as to maximize rehydration without compromising the sensory properties of the beverage. In addition, by including potassium, magnesium and chloride at especially advantageous levels, the rehydration effects are further improved without changing the palatability of the beverage. Furthermore, by maintaining the combination of extracellular-favoring ions at a level of between about 40-78 mEq/L, the advantageous rehydration and sensory properties are promoted.
- The advantages discussed above have been demonstrated through a number of experiments. Various formulations were tested for various sensory properties. In addition, these formulations were tested for rehydration ability through the mechanisms of increased voluntary fluid consumption, increased fluid retention, and reduced urine loss. The first experiment to determine the taste characteristics of the beverage was performed on fifty athletes at rest. Of these experimental formulations, the preferred one had a sodium content of 30 mEq/L., had very good hedonic ratings, consistent with the leading sports drink in the U.S.
- Improved voluntary fluid consumption of this formulation also has been shown. An experiment was conducted on 50 athletes that were allowed to drink ad libitum during an exercise protocol. This formulation was directionally favored over other sodium levels for this beverage in terms of voluntary fluid consumption. Another finding was that “salty” beverages become more palatable during and after exercise compared to at rest. In addition, dehydration was abated to a higher degree with this formulation.
- Other testing was directed to fluid retention and urine-loss effects. Subjects were deprived of fluid during an exercise-induced dehydration period resulting in a loss of 2.5-3.0% of body weight. Following the exercise-induced dehydration, the subjects drank enough of the beverage formulations to equal the fluid lost through sweat. Results indicated significantly greater fluid retention with the experimental formulation having 30 mEq/L of sodium. Similarly, this formulation outperformed other experimental formulations in attenuating fluid loss in the form of urine. In addition, this formulation scored directionally higher hedonic ratings. While this formulation was liked equally well as another experimental formulation having 18 mEq/L of sodium, it was directionally favored over all other experimental formulations having different sodium levels for characteristics of sweetness, flavor, tartness, saltiness and overall acceptance.
- Two more experiments were conducted in a similar fashion and with a similar purpose. The experimental formulation having 30 mEq/L of sodium remained the same. It was compared to different experimental formulations. In addition, blood and urine chemical analyses were conducted. The experimental formulation having 30 mEq/L of sodium had improved rehydration properties; urinary fluid loss was reduced, the percentage of fluid retained increased, and overall body weight change was greater.
- Additionally, two experiments were conducted to determine the effects on rehydration, using similar methods, by combining sodium and chloride at certain concentration levels. The first of these experiments compared the 30 mEq/L of sodium formulation described above with experimental formulations containing 18 and 25 mEq/L of sodium. The 25 mEq/L formulation included an amount of chloride so that the combined level of these extracellular favoring ions was substantially higher than both the 30 or 18 mEq/L of sodium formulations. (See the formulation table in example 6). The 25 mEq/L formulation resulted in improved fluid retention compared to the other two formulations.
- The second of these experiments compared the 30 mEq/L of sodium formulation with a 25 mEq/L of sodium formulation, but this time the combined levels of sodium and chloride were more closely matched in the two formulations. (See the formulation table in example 7). No differences in fluid retention were found between these formulations. However, the 25 mEq/L of sodium formulation received initial negative sensory ratings versus the formulation having 30 mEq/L of sodium, but less chloride.
- The beverage of the present invention typically includes from about 4% to about 10%, preferably from about 5.5% to about 6.5%, more preferably about 6% by weight of a carbohydrate source. Carbohydrate sources, include but are not limited to, sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate. Preferably, a mixture of a minimum of three of these is formed, with the amount of fructose being less than the total amount of glucose from all carbohydrate sources. A preferred composition of carbohydrates comprises from about 1% to about 5% sucrose, from about 1% to about 2.5% glucose and from about 0.8% to about 1.8% fructose to produce a total of 6% carbohydrate, and more preferably from about 2 to about 4% sucrose, from about 1.4 to about 2% glucose, and about 1.1 to about 1.5% fructose, to produce a total of 6% carbohydrates.
- The sodium content of the beverage of the present invention comprises at least about 30 mEq/L, preferably from about 30 to about 100 mEq/L of beverage, more preferably from about 30 to about 60 mEq/L of beverage, even more preferably from about 33 to about 40 mEq/L. This sodium concentration indicates the total amount of sodium present in the beverage, including sodium contained in the carbohydrate source, flavoring agent (to the extent known), and clouding agent. For example, maltodextrin as a carbohydrate source may contain sodium. However, these sources alone cannot raise the sodium levels of the beverage to the necessary levels, and as such additional sodium must be added from another sodium ion source.
- Any source of sodium known to be useful to those skilled in the art can be used in the present invention. Examples of useful sodium sources include, but are not limited to, sodium chloride, sodium citrate, sodium bicarbonate, sodium lactate, sodium pyruvate, sodium acetate and mixtures thereof. A mixture of sodium chloride and sodium citrate being preferred, and a mixture of from about 10 to about 50 mEq/L, preferably from about 10 to about 30 mEq/L, and more preferably from about 10 to about 20 mEq/L of sodium from sodium chloride and from about 10 to about 50 mEq/L, preferably from about 10 to about 30 mEq/L, and more preferably from about 10 to about 20 mEq/L of sodium from sodium citrate.
- In addition, the beverage of the present invention also includes chloride. The chloride ion can come from various sources known to those skilled in the art. Examples of chloride sources include, but are not limited to, sodium chloride, potassium chloride, magnesium chloride and mixtures thereof. The concentration of chloride is at least about 10 mEq/L, preferably from about 10 to about 20 mEq/L, more preferably from about 11 to about 18 mEq/L of chloride from sodium chloride.
- Furthermore, the beverage of the present invention also preferably includes a combination of extracellular favoring ions, where the sum of these ions is from about 40 to about 78 mEq/L. This range can even be from about 42 to about 70 mEq/L or from about 46 to about 60 mEq/L. Sodium and chloride ions are some of the ions which favor the filling of the extracellular fluid compartment.
- The beverage of the present invention also includes potassium. The potassium ion source can come from many sources known to those skilled in the art as being useful in the present invention. Examples of potassium sources useful herein include, but are not limited to, potassium monophosphate, potassium diphosphate, potassium chloride, and mixtures thereof, with potassium monophosphate being preferred. The potassium content is at least 8 mEq/L, preferably from about 8 to about 20, and more preferably at from about 10 to about 19 mEq/L.
- The beverage of the present invention further preferably includes magnesium. The magnesium ion can also come from many sources known to those skilled in the art. Examples of magnesium sources include, but are not limited to, magnesium oxide, magnesium acetate, magnesium chloride, magnesium carbonate, magnesium diphosphate, magnesium triphosphate, magnesium in the form of an amino acid and mixtures thereof, with magnesium oxide being preferred. The concentration of magnesium is at a level of at least 0.1 mEq/L, preferably from about 0.5 to about 6 mEq/L, more preferably from 1 to 3 mEq/L.
- Additionally, calcium preferably is present in the beverage of the present invention. The calcium ion may come from a variety of sources known to those skilled in the art. Examples include but are not limited to, calcium lactate, calcium carbonate, calcium chloride, calcium phosphate salts, calcium citrate and mixtures thereof, with calcium lactate being preferred. Calcium is present at a concentration of at least 0.1 mEq/L, preferably from about 0.5 to about 6 mEq/L, more preferably from 1 to 3 mEq/L.
- A flavoring agent may be used in the beverage of the present invention. The flavoring agent of the beverage of the present invention also impacts the overall acceptance of the beverage. In order to achieve this overall acceptance, the strength of the flavor cannot be too intense. Of course, the flavor intensity of the beverage will depend upon the amount and type of the particular flavoring agent used. Furthermore, the same flavor from different suppliers may have differing intensity. Thus, it is difficult to quantify the level of flavoring agent necessary for the present invention. However, in general it has been found that a flavoring agent at a concentration of from about 0% to about 0.400% by weight is useful in the present invention. Furthermore, flavoring agents themselves may contain gum arabic, ester gum, starches such as, dextrins, “modified food starch”, propylene glycol or alcohol. These additional components may acts as carriers or stabilizers.
- In addition, any flavoring agent which satisfies the above criteria and is known to be useful to those skilled in the art can be used in the present invention. Examples of particularly useful flavoring agents, include but are not limited to, lemon-lime, orange, and fruit punch. For example, the lemon-lime flavoring agent can be at a concentration in the range of from about 0.050% to about 0.200%, preferably from about 0.080 to about 0.150%, and more preferably from about 0.090 to about 0.120% by weight.
- The beverage of the present invention is formulated to have an osmolality, when initially formulated, in the range of from about 220 to about 350 mOsm/Kg of beverage, and is preferably in the range of from about 250 to about 330, more preferably from about 260 to about 320 mOsm/Kg of beverage. When prepared the beverages of the present invention are isotonic. The scientific and strict definition of the term isotonic is a solution that has the same or nearly the same osmotic pressure as another solution, typically human blood. Although the beverages of the present invention can be isotonic when prepared, even with regards to the strict scientific meaning of the term, the term isotonic as presently used is not meant to be so narrowly defined. With respect to the present specification, isotonic is meant to refer to the fact that the beverages of the present invention are sports type beverages which contain a certain amount of carbohydrates and electrolytes.
- The beverage of the present invention may also include a clouding agent at a concentration range of from about 0 to about 100 ppm of clouding agent. Examples of clouding agents include, but are not limited to, ester gum, SAIB, starch components and mixtures thereof, with ester gum as the preferred clouding agent at a concentration range of from about 10 to about 50 ppm and more preferably from about 15 to about 35 ppm.
- The beverage of the present invention may further include citric acid at a concentration range of from about 0.24% to about 0.45% by weight. Citric acid lowers the pH in order to insure it is a high acid beverage which may be pasteurized under conditions less harsh than required for low acid beverages. Beverages of the present invention preferably have a pH of from about 2.5 to about 4.5, preferably from about 2.75 to about 4.25, more preferably from about 2.9 to about 4.0. In addition, citric acid adds tartness to the beverage.
- The present invention also relates to a beverage concentrate used to prepare the beverage already described herein. As used herein, the term “beverage concentrate” refers to a concentrate that is either in liquid or gel form or in essentially dry mixture form. The essentially dry mixture is typically in the form of a powder, although it may also be in the form of a single-serving tablet, or any other convenient form. The concentrate is formulated to provide a final and complete beverage as already described herein when constituted or diluted with water or other liquid.
- A preferred beverage concentrate of the present invention capable of producing the preferred beverage below when constituted or diluted with water comprises:
-
- from about 4% to about 10% by weight of carbohydrates;
- at least 30 mEq/L of beverage of sodium with a preferred range of 30-60 mEq/L;
- at least 8 mEq/L of beverage of potassium with a preferred range of 8-20 mEq/L of beverage of potassium;
- from about 10 to about 20 mEq/L of beverage of chloride;
- from about 0% to about 0.4% of a flavoring agent;
- from about 0 to about 100 parts per million(ppm) of a clouding agent; and
- water wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg. The carbohydrates can be selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan and/or trioses such as pyruvate and lactate, preferably as a mixture of at least three of these. Preferably, the amount of fructose is less than the total amount of glucose from all sources of carbohydrates.
- The present invention is further illustrated, but not limited by, the following examples.
- A formulation designated as G30 was evaluated for sensory properties against four other formulations containing the typical ingredients found in commercial sports drinks. The G30 formulation consisted of a 6% carbohydrate solution in water, 30 mEq/L of sodium, 3 mEq/L potassium, about 10 mEq/L of chloride, 25 ppm of a clouding agent and 0.103% by weight of a flavoring agent. The carbohydrate solution was prepared using a
mixture 3% sucrose, 1.7% glucose, and 1.3% fructose. In addition, both sodium chloride and sodium citrate were used to provide the sodium ions. The comparison formulations contained the exact ingredients except that the sodium levels of these beverages were modified so that the first formulation, designated as G0, contained 0 mEq/L of sodium, the second formulation, designated as G18, contained 18 mEq/L of sodium, the third formulation, designated as G40, contained 40 mEq/L of sodium, and the fourth formulation, designated as G60, contained 60 mEq/L of sodium. - Approximately 50 triathletes and runners completed this phase of testing. Tasting was done in a sedentary condition in a sensory laboratory. Under sedentary conditions, liking of the saltiness level and liking of the beverage overall peaked at 18 mEq/L. However, the difference between the ratings for G18 and G30 were not statistically significant. As is shown in
FIG. 1 , the G30 formulation had hedonic ratings which surpassed all but the G18 formulation. - The same formulations were used to determine the ad libitum drinking characteristics, sensory aspects, hydration characteristics, and voluntary fluid intake for each. Fifty subjects completed almost all testing and were included in the subject pool used for analyses. Of this 50, 28 were men (m age 39.7±8.0) and 22 were women (m age 37.2±9.2). For the complete pool, the average number of workout hours per week was 11.25±6.8 (range 1-35) and the average number of races participated in per year was 11.0±8.4 (range 0-35). All subjects were given dietary and exercise guidelines as follows: No heavy exercise on the day before testing. Allow at least one full day of recovery between sessions. No caffeine the day of testing. No high salt or high fat foods the evening before or the day of the testing. Do not eat within 3 hours of testing. Drink a liter of
water 3 hours before testing. Void urine prior to testing. - All subjects were required to follow the diet and exercise guidelines prior to participating in the experiments. In order to assure pre-experiment hydration, 1 liter of fluid was given for each subject to finish consuming by 1.5 to 2 hours before starting the protocol. A protocol was performed to create 2 hours of moderate aerobic exercise in the heat (80° F., 36% RH, 21° C. WBGT) at 70-75% max age-predicted heart rate. Subjects were given approximately 700 ml of fluid per bottle, and they received a fresh, cold bottle each 20 minutes for the entire two hours. Subjects were allowed to drink freely until the end of exercise, at which time no further fluid was provided. All urine samples were collected during each 20-minute segment and again 15 minutes after the exercise session ended. Subjects were asked to urinate as frequently as they felt necessary, and during the protocol.
FIG. 2 illustrates the procedure employed. - As the chart in
FIG. 3 indicates, the total volume of fluid consumed was directionally greatest for G30 and least for G0. However, the main effect for sodium level (product) was only marginally significant (p=0.058). There was no real meaningful difference in the total voluntary fluid intake between beverages. This suggests, in part, that the subjects being athletes were well trained to drink. - Post-exercise urine volumes indicated an inverse association between sodium level and urine volume produced, which is indicated in
FIG. 4 . Urine volumes were directionally less as sodium level increased from 0 to 60 mEq/L and was least for G60 at 15 minutes post-exercise compared to other beverages. Analysis of the trend in the data (e.g. slope) for post-exercise urine excretion was significantly different from 0. That is, there is 95% confidence that the downward trend in the data was not due to chance alone. Overall, exercise urine volumes and total urine volumes were similar among beverages. - The level of dehydration was similar (p=0.354) among all beverages, due to the fact that all beverages were voluntarily consumed in about similar volumes. It was directionally the least for G30 at 1.06% and the most for G0 at 1.23% as shown in
FIG. 5 . On average, HR and RPE were not different between beverages, but did increase over time of exercise. - Overall acceptance (OA) of the beverages was greatest for G18 and G30 and differed significantly from the other beverages (p<0.05). The G18 and G30 beverages did not differ from each other with respect to OA. Liking of saltiness was greatest for G18 and G30 and was significantly different (p<0.05) from 0, 40, and 60 mEq/L beverages. The liking of saltiness in G18 and G30 did not differ from each other. There was also a significant interaction effect between sodium level and time. Liking of saltiness decreased over time for G0 and G18, whereas it increased for G30, G40, and G60 over time. All sodium levels were perceived to be different (p<0.05), increasing in perceived salt intensity as sodium level increased.
- Ideal saltiness ratings were similar across all beverages, ranging from 39 to 43.5 mm (0-100 mm scale) This corresponded to a sodium level lying somewhere between G18 (38±1.5) and G30 (47±1.7). Plotting this data, ideal perceived saltiness would correspond to a sodium level of 24 mEq/L as shown in
FIG. 6 . - A comparison of overall acceptance and liking of saltiness from sedentary to exercise conditions revealed a decline in the curve for OA with increased sodium level softened, meaning thus, at higher sodium levels (30, 40, 60 mEq/L), hedonic scores recorded at 60 minutes during the exercise were higher compared to the scores recorded in sedentary conditions. For liking of saltiness (LOS), the same response was observed, with the curve peak shifting from 18 mEq/L to 30 mEq/L. These results are illustrated in
FIG. 7 . - For both OA and LOS up to a sodium level of 60 mEq/L, hedonic scores never crossed into the “dislike” region of the scale during exercise. Ideal saltiness and perceived saltiness did not change significantly or meaningfully between sedentary and exercise conditions. There was an upward directional shift in perceived saltiness associated with the exercise condition.
- The conclusions gathered from the sedentary and exercise testing indicated that moderate to high levels of sodium in G30 are not associated with any GI distress or associated symptoms during two hours of moderately intense exercise in the heat. Well-trained athletes will drink beverages that are not optimally palatable in order to preserve performance and hydration. Even though the G30 beverage is perceived to be more salty than G18, it appears that from a hedonic standpoint, 30 mEq/L of sodium is liked, is tolerable, and is even directionally favored over both higher sodium levels and no sodium in the other formulations. Acceptance is broadened and liking of sodium is increased in higher (30-60 mEq/L) sodium beverages during exercise compared to at rest.
- This study compared all the formulations except the G40 formulation to determine how rapidly fluid is lost and how much is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss. This was conducted in a double-blind experimental design with formulations counterbalanced among the study group.
- Subjects were fed a standard diet for dinner (the evening prior to testing), breakfast, and lunch. The total caloric intake was 2440 calories and 2592 mg of sodium. Meals were accompanied with 500 ml of water.
- All subjects were required to follow the diet and exercise guidelines prior to participating. In order to assure pre-experiment hydration, each subject was required to ingest adequate fluid. Pre-experiment hydration was further assured by checking the conductivity/osmolality of the baseline urine sample prior to the testing. Subjects whose urine sample exceeded 21 milliSiemens (mS) were dismissed from the experiment and required to reschedule.
- Subjects were asked to complete 90 minutes of moderate-intensity cycling exercise (or enough exercise to result in a loss of approximately 2.5-3.0% of body weight) in a warm environment (80° F., 40% RH). Following exercise, they rested quietly for 3.5 hours. During the first 90 minutes of recovery, subjects drank a volume of fluid equal to 100% of their total sweat loss during the exercise-dehydration period. Over the entire five-hour period, urine samples and perceptual data were collected periodically according to the protocol. The summary of this protocol is illustrated in
FIG. 8 . - Ten subjects completed four trials. Average loss of bodyweight during the exercise-induced dehydration was 2.6±0.6% over 1.5 hours. Dehydration did not differ across formulations.
- At the end of the experiment, the average total urine losses were 0.546, 0.430, 0.322 and 0.287 liters for 0, 18, 30, 60 mEq/l beverages, respectively as shown in
FIG. 9 . Both 0 and 18 were significantly (p<0.05) different from 30 and 60. The 30 and 60 beverages did not differ from each other. Differences in urine loss did not become apparent until 1 hour after the end of the drinking period. By 2 hours after the drinking ended (3.5 hrs post-exercise), differences among 0, 18, and the other beverages were greatest. Average fluid intakes ranged from 1.8 to 1.95 liters, but were not different across formulations. Two hours after rehydration with a volume equivalent to 100% of total sweat lost, the total percent of fluid retained was 69%, 75%, 82%, and 83% for 0, 18, 30, and 60 mEq/L, respectively as shown inFIG. 10 . - The G30 beverage scored directionally higher than all other beverages. Sensory evaluation indicated that the 30 mEq/L was liked equally well as G18, but was directionally favored over all other beverages for characteristics of sweetness, flavor, tartness, saltiness, and overall acceptance. For the G18 and G30 beverages, ratings of perceived saltiness were very close to ideal ratings of saltiness throughout the drinking period. In general, hedonic scores declined with increasing time (30 to 90 min) during the rehydration period.
- Ratings of ideal saltiness were consistent with results demonstrated in Example 2 (n=50), but were slightly higher (43-50 vs. 40-42). In most cases, perceived saltiness for the G30 beverage was scored near to ideal saltiness ratings for that beverage. As consumption continued past 60 minutes, perceived saltiness levels of the beverages became less than ideal. The variation between perceived and ideal saltiness was greater for the G0 and G60 formulations, than for control G18 or G30.
- There were no marked differences in perceived alertness, energy, GI assessments or well-being among the four beverage trials.
- The G30 formulation performed directionally better than G18 for a number of hedonic ratings and provided a greater fluid retention response during rehydration than other beverages. The highest sodium level in the G60 formulation provided no further benefit beyond the G30 formulation in terms of attenuating fluid loss in the form of urine.
- The conclusions gathered indicate that G30 provides the following advantages over G0, G18, G60: (1) directionally higher hedonic ratings in athletes undergoing exercise in the heat and (2) attenuated urinary fluid losses after rehydration with a volume equivalent to 100% of sweat loss.
- The same formulations as Example 3 were used except that a G5 formulation was included and the G0 and G60 formulations were eliminated. The G5 formulation was identical to the other formulations except that the sodium level was adjusted to 5 mEq/L.
- This testing compared the formulations to determine how rapidly fluid is lost and how much is retained up to three hours after exercise-induced dehydration that is followed by replacement of total sweat loss. Certain biochemical parameters were measured to determine physiology related changes. The parameters measured were blood related changes in [Na+], [K+], [Ca++], osmolality, Hb, Hct, ΔPV, glucose, pH, and urine related changes in volume, osmolality, [Na+], [K+], SEC, FWC, creatinine, and GFR. This was also conducted in a double-blind experimental design with formulations counterbalanced among the study group.
- Seventeen (n=17) male subjects dehydrated by 2.25±0.61% of bodyweight during 1.5 hours of cycling at 70% of maximum heart rate in a warm (80° F., 40% RH) environment. Subjects were fed a standard diet for dinner the evening prior to testing, and for breakfast and lunch the day of the test. The total caloric intake was 2440 calories and 2592 mg of sodium. Additional liquids (within guidelines) were permitted until 3 hours prior to the testing.
- In order to assure pre-experiment hydration, each subject was required to ingest fluid adequate to establish euhydration. This included a minimum intake of 500-ml of water the evening prior to the test as well as 1-liter of water the day of the test. Pre-experiment hydration was further assured by checking the conductivity of the pre-experiment urine sample prior to the experiment. Subjects whose first experimental urine sample had a conductivity that exceeded 21 mS-cm were dismissed from the experiment and required to reschedule. An indwelling venous catheter was inserted into a forearm vein for repeated blood sampling throughout the experiment.
- Subjects cycled in a warm environment (80° F., 40% RH) for 1.5 hours to elicit an exercise-induced dehydration near two percent of initial bodyweight. A previously determined workload was used to set an exercise intensity equivalent to 70-75% of maximal heart rate as determined by earlier maximal treadmill tests. Heart rate was monitored every 10 minutes using a telemetered HR monitor (Polar) and to assure sufficient intensity of exercise.
- At 1-hour, subjects were allowed a 5-minute break from cycling during which time a dry weight was measured to determine sweat loss progress. At the end of the exercise-dehydration period, a 15-cc pre-recovery blood sample was taken while upright on the bike, and subjects urinated and were weighed again to determine final sweat loss. The subjects were then seated in a reclined position for the remainder of the recovery period. Where appropriate, the subject's arm containing the indwelling catheter was wrapped in a heating pad to maintain blood flow and vessel dilation that provided for better blood sampling in a dehydrated state.
- Beverage was mixed prior to the experiment using 1-liter and 2-liter volumetric flasks and beverage grade water that did not contribute any electrolytes to the beverage. Beverages were served chilled from the refrigerator (approx. 40° F.), although the last two beverages were likely to be closer to ambient temperature by the time they were served to the subjects.
- At 30 minutes into recovery, subjects received their first of six cups of beverage, followed by another cup every ten minutes. Total beverage volume was equivalent to total sweat loss, and was portioned such that subjects received 50% of the total volume in the first 20 minutes and the remaining 50% in 12.5% portions every ten minutes for the remaining 40 minutes. All fluid was consumed within an hour.
- Every 30 minutes during the 3.5 hour recovery, blood and urine samples were taken. The testing protocol and sequence are shown in
FIG. 11 . - A whole blood clinical analyzer (Instrumentation Laboratories, Synthesis IL1735) was used to measure changes in blood sodium, potassium, ionized calcium, glucose, hematocrit, hemoglobin, and pH value. Approximately 1-ml of whole venous blood was collected in a 3-ml heparinized arterial blood-gas syringe (Marquest, GASLYTE) and was used specifically for the clinical analyzer. The arterial blood gas syringe was chosen to avoid sample clotting and to ease presentation of the sample to the clinical analyzer.
- Plasma volume changes were estimated from a published equation (Dill/Costill) relating hematocrit and hemoglobin changes. Plasma osmolality was measured by freezing point depression method on a FISKE 2400 multiple sample osmometer.
- Urine volume was determined by weight, and urine conductivity was immediately determined using a conductivity meter (WTW LF 340, model 19706-20). Urine was then aliquoted and stored at −20° F. until later analysis for [Na+], [K+], osmolality and creatinine. Urine [Na+] and [K+] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Urine creatinine was determined on the spectrophotometer utilizing a Sigma Creatinine Kit (Sigma Diagnostics, No. 555). Urine osmolality was determined by the freezing point depression method on an osmometer after thawing frozen samples to room temperature.
- Data were analyzed using a computer statistics package (SPSS, v.10). Descriptive statistics and summary tables were generated for all dependent measures. Repeated measures ANOVA was used to test for main effects of treatment and where appropriate time dependent effects. Where main effects existed, a Duncan post-hoc test was used to determine statistical differences among means. Comparisons were tested with an alpha level equal to 0.05. Effect size estimates were made for comparisons of total urine loss between formulations.
- Seventeen subjects (N=17) completed all three trials, with the exception of one subject who became ill (unrelated to the study), but was forced to discontinue the study after already completing two trials. Average loss of bodyweight during the exercise-induced dehydration was 2.25±0.61% over 1.5 hours. The subjects exhibited an average sweat rate of 1.2±0.3 liters/hr and lost an average of 1.7±0.5 liters of sweat during the dehydration period. Dehydration and sweat loss did not differ across formulations.
- During the recovery period, total urine loss was significantly lower for G30 (298±161 g) compared to G5 (479±209 g) and G18 (408±151 g) formulations, but this effect was time dependent. It was not until 120 minutes in recovery (30-min post-drinking) did the effect of G30 become different from G5 and G18.
FIG. 12 shows cumulative urine loss among the three formulations across recovery time. Effect sizes estimates for total urine loss were 0.39 (G5 vs. G18), 0.97 (G5 vs. G30), and 0.70 (G18 vs. G30). - Percent of fluid retained was significantly higher (P<0.05) for G30 versus G5 and G18 at 150 through 210 minutes in recovery. Thus, at the end of the recovery period (2 hours post-consumption), fluid retention was 72.6%, 75.2%, and 81.6% for G5, G18, and G30, respectively.
FIG. 13 shows fluid retention during recovery for the three formulations tested. - Absolute change in bodyweight from baseline weight was significantly greater for G5 compared to G18 and G30 (P<0.05). Bodyweight losses, corrected for any fluid gains during recovery, were 0.93, 0.80 and 0.74 kg, respectively.
- There were no treatment effects on plasma [Na+], [K+], [Ca++], or glucose. Additionally, change in plasma volume (DPV) as calculated from hematocrit and hemoglobin was not different among the three formulations. Change in plasma volume among the three formulations is shown in
FIG. 14 . - ANOVA analysis revealed a main effect of sodium (P<0.001) and time (P<0.0001), with an interaction between sodium and time (P<0.0001) on individual urine volumes excreted. Independent of time (summed across time points), average urine volumes were 79.8, 68.0, and 49.7 g for G5, G18, and G30, respectively.
- Urine conductivity varied by sodium level and time, with an interaction between sodium and time (P<0.01). Independent of time (summed over all time points), average urine conductivity was 12.3, 12.8 and 14.8 mS-cm for G5, G18, and G30, respectively. Conductivity was significantly higher for G30 compared to G5 and G18.
- Urine sodium and potassium concentrations were not different between sodium levels, but did differ by time. Urine sodium and potassium excretion, corrected for volume, did not differ between formulations, but did differ over recovery time. When summed across formulations, average [Na+] excreted was significantly higher at 0 minutes recovery (7 mEq), dropped to 4.4 mEq at 30 minutes into the drinking period, and remained between 2.2-2.4 mEq from 90 through 210 minutes of recovery.
- To examine any sensory (taste) and perceptual changes associated with the various formulations, the subjects rated a number of psychophysiological characteristics on several different scales (e.g. categorical, 100 pt) throughout the experimental period.
- Overall acceptance of beverages was not significantly different among formulations. The G30 beverage significantly declined in overall acceptance, and liking of flavor, saltiness, sweetness, and aftertaste, particularly at the end of the 1-hr drinking period. Acceptance of the G5 and G18 products did not decline over time. There were no differences in perceived saltiness between G5 and G18, but G30 was perceived to be saltier at two of the four evaluation times (32 and 62 minutes). The effect was not consistent.
- Beverage treatment had no effect on ratings of ideal saltiness as ideal saltiness consistently ranged from 47 to 51 pts on a 100-pt scale. Perceived saltiness did not differ from ratings of ideal saltiness for G5 and G30, but G18 was rated significantly less salty than ideal at both 32 and 62 minutes. Again, this effect was not consistent across time.
- There were no differences in perceptions of physiological or psychological well-being among formulations. Perceived energy, alertness, and feelings of well-being were similar with all formulations, but did vary over time consistent with the experimental protocol. At the end of the experiment, the G30 product resulted in significantly lower ratings for hunger compared with G5 and G18. There were no other treatment effects on any of the ratings of perceived gastrointestinal stress (bloating, fullness, nausea, or urge to urinate).
- Although there were no differences in perceived thirst among products, subjects consuming the G18 product were directionally more thirsty than those consuming either G5 or G30.
- For the same purposes as the Example 4, the G30 formulation was compared to water (W), a commercial product POWERade® (P), and a G18 formulation identical to the G30 formulation except that the sodium level was adjusted to 18 mEq/L. Specific electrolyte formulations are shown below.
[Na+], [K+], [Cl−], Osm Code mEq/L* mEq/L* mEq/L (mOsm/kg)* W 0 0 0 0 P 5 2 5.8 428 G18 18 3 9 320 G30 30 3 9 334
*Indicates measured analytical values
- Beverage temperature was maintained between 41-45° F. and served at that temperature and the beverages were blinded to the subjects as well as the experimenters.
- Subjects included trained men (ages 25-50 y). Seventeen completed both trials. Testing followed a 3-hour fast (drinking and eating).
- Workloads were set for the cross-trainer, stationary bike and treadmill sufficient to produce intensities between 70-75% of their measured maximum heart rates (determined from annual stress tests). In addition, body weights were obtained prior to and following the orientation exercise session in order to predict sweat rates.
- Subjects were provided with standardized meals to ensure consistent sodium intake (˜2900 mg) prior to each of the two trials. Meals included dinner (evening prior to experiment), and breakfast and lunch on the day of testing. Subjects were given the option of eating all or a portion of the food provided but were instructed to record which foods and amount left uneaten. These items were then withheld from the food bags for the next trial. In addition, they were given bottled water to drink the evening before (500 ml) and during the testing day (1000 ml) to ensure adequate hydration. Subjects were asked to refrain from caffeine and alcohol use for 24-hours prior to the experiment. Most subjects ate all the food provided to them, however, intakes ranged from 2,109-2,278 kcal and 2,849-2,960 mg sodium. None of the subjects ate food other than what was provided.
- The exercise session consisted of 30-minutes each on the cross trainer, stationary bike and treadmill at 75-80% maximum heart rate for a total of 90 minutes. Heart rates were taken at 15-minute intervals to ensure subjects maintained adequate intensity. Subjects refrained from drinking during the entire exercise period in an effort to produce 2-2.5% dehydration.
- Following exercise, subjects were weighed and gave a post-exercise urine sample, followed by a 3.5 hour recovery period. At 30 minutes into recovery, subjects were given their first beverage equivalent to 25% of their total sweat loss (determined by subtracting post-exercise weight from pre-exercise weight). A beverage (25% of losses) was given again at 40 minutes into recovery. The third aliquot of beverage, equivalent to 12.5% of sweat loss, was given at 50 minutes. At 58 minutes, subjects were given a GI scale to assess gastro-intestinal responses and a urine sample was taken. After which they were given their fourth aliquot (12.5%). The fifth aliquot (12.5%) was given at 70 minutes, and the sixth and final aliquot (12.5%) was given at 80 minutes. Sensory questionnaires were filled out periodically. At 90, 120, 180 and 240 minutes, GI scales were given and urine samples were collected. After the final urine sample, final body weights were measured.
- Urine volume was determined by weight and urine specific gravity was measured (A 300 Clinical Refractometer). Urine was the transferred into 4-ml cryovials for further analysis. Urine [Na+] and [K+] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- SPSS version 10.0 was used to analyze the data. ANOVA using a general linear model was used to determine differences among mean values. Data is reported as the mean+the standard deviation.
- The 90-minute exercise session resulted in similar levels of dehydration among formulations (2.67±0.63%, 2.71±0.64%, 2.61±0.43%, and 2.61±0.54% for W, P, G18 and G30, respectively). In addition, sweat rates among formulations were essentially the same (1.34±0.40 L/hr, 1.36±0.38 L/hr, 1.25±0.28, 1.30±0.33 L/hr W, P, G18 and G30, respectively).
- Total fluid intake among formulations was also similar between trials at 2.01±0.60 L for W, 2.03±0.57 L for P, 1.88±0.41 L for G18 and 1.95±0.50 L for G30. The volume administered varied slightly in mean value because of the modest variability in sweat rate and the protocol of replacing 100% of the total sweat losses.
- Final body weights as percentage of initial body weights differed among formulations. Subjects returned to 98.45+0.29% of initial body weight following the water trial, significantly less than the other three formulations. POWERade® (98.72±0.37%) and G18 (98.87±0.28%) were not different from each other. In addition, G18 was not different from G30 (98.97±0.39%). G30 was significantly different from water and POWERade®.
- Total cumulative fluid output was significantly different among formulations. W resulted in significantly more urine loss than the other three formulations (0.726±0.225 L). P and G18 were not significantly different from each other at 0.496±0.184 L and 0.428±0.196 L, respectively. G30 was different from W and P, but not from G18 (0.367±0.263 L). Urine output at each data collection point was not significantly different among formulations for
time points time point 180, W resulted in greater urine loss compared to the other three formulations. Additionally, attime point 240, W and P resulted in greater urine loss than G18 and G30 (See TABLE I, values being reported in milliliters collected at each of the time points.)TABLE I 60 90 120 180 240 Treatment minutes minutes minutes minutes minutes Water 40.72 ± 24.95 35.32 ± 22.46 115.94 ± 55.65 349.39 ± 152.72* 184.77 ± 122.51{circumflex over ( )} POWERade ® 38.29 ± 26.86 32.75 ± 22.51 76.45 ± 54.42 182.16 ± 84.43 166.08 ± 107.67{circumflex over ( )} G18 49.73 ± 42.37 30.68 ± 21.71 100.75 ± 62.70 151.57 ± 93.18 95.07 ± 59.40 G30 35.05 ± 25.26 24.30 ± 15.42 82.21 ± 72.17 133.14 ± 153.26 92.07 ± 69.09
Each time point represents the number of minutes into recovery.
*Denotes significantly more urine loss than P, G18, and G30.
{circumflex over ( )}Denotes significantly more urine loss than G18 and G30.
- Fluid retention, or the amount of fluid consumed that was not excreted as urine, was different among formulations. This was calculated in both relative terms (ml/kg) and as a percentage calculated as (fluid in−fluid out)/fluid in. Relative fluid retention for W was 17.07±5.22 ml/kg, significantly less than the other formulations. P, G18, and G30 were 20.43±6.50 ml/kg, 19.48±4.83 ml/kg, and 21.26±4.83 ml/kg, respectively. Percent fluid retained was different among formulations (62.94±9.05% for W, 74.25±11.15% for P, 76.52±10.32% for G18, and 81.45±10.34% for G30) with W significantly less than P, G18 and G30 at
minutes FIG. 15 . - Urine produced during the recovery period was different among formulations. As depicted by
FIG. 16 , W (x=134.97 ml) was significantly different (greater urine volume) than P (x=98.99 ml), G18 (x=91.64 ml) or G30 (x=86.38 ml). G30 resulted in significantly less urine production than W and P. G18 was not different than P or G30. SeeFIG. 16 . - Average urine osmolality was different among formulations. W resulted in the lowest urine osmolality (348.33 mOsm) and was not different from P (400.66 mOsm) but was significantly different from G18 (431.74 mOsm) and G30 (500.42 mOsm). G18 was not different from P or G30. P was significantly less than G30. See
FIG. 17 . - Average urine sodium concentration was significantly greater for the G30 trial (65.19±45.68 mEq/L) compared to the other trials (W=49.73±39.78; P=50.14±41.93; G18=52.48±40.44). None of the other trials were different from each other. See
FIG. 18 . - The G30 trial resulted in significantly more potassium loss (52.25±36.56 mEq/L) compared to the other trials (W=39.67±38.15; P=41.82±40.42; G18=41.58±33.97). The other trials did not differ from each other. See
FIG. 19 . - Subjects began each exercise session well hydrated (USG=1.008±0.005). Urine specific gravity did not differ among formulations prior to exercise. Average USG for W treatment was significantly less than P at
minute 120. Additionally W was less than G18 and G30 atminute 180. USG for P was significantly less than G30 atminutes FIG. 20 . - The estimated acute sodium balance is shown in TABLE II.
TABLE II Estimated Intake Out (mEq) Total Percent (mEq) Sweat* Urine{circumflex over ( )} Loss Replaced Water 0 131.15 15.51 ± 7.11 146.66 0 POWERade ® 10.19 132.80 13.52 ± 9.42 146.32 6.96 G18 33.86 122.58 15.65 ± 9.95 138.23 24.50 G30 58.59 127.24 14.79 ± 8.88 142.03 41.25
*Sweat losses estimated from an average of sweat patches placed on each forearm during one of the four exercise sessions. The amount was calculated by multiplying concentration by volume of sweat lost for a given night.
{circumflex over ( )}Cumulative loss determined from the sum of the concentration of sodium in each urine sample multiplied by the volume of each sample. No differences were found in mean urine sodium loss between formulations.
- Urine protein was measured via reagent strips (Uristix) to determine whether or not dehydration has an effect on protein excretion. TABLE III is a chart indicating the frequency and amount of protein detected in the urine at each time point for each of the four formulations.
TABLE III Protein Treatment Time (mg/dl) Water POWERade ® G18 G30 Pre- Negative 16 17 17 17 Exercise Post- Negative 16 13 10 13 Exercise Trace 0 3 5 3 60 Negative 9 8 10 8 Trace 6 5 5 5 30-100+ 1 2 0 3 100+ 1 1 90 Negative 10 12 11 9 Trace 4 4 0 5 30-100 1 1 0 2 100+ 1 120 Negative 16 16 11 13 Trace 0 1 1 4 30-100 1 180 Negative 15 16 10 13 Trace 0 0 1 2 240 Negative 15 16 10 11 Trace 0 0 1 5 - In regards to sensory acceptability, the differences between G30 and G18 were few and rather random. Although the G30 formula was initially less acceptable than the G18 for overall acceptance, flavor, sweetness, and tartness at the initial sensory read (32 minutes into recovery time), the differences between these two beverages decreased rapidly with time. While G18 generally had a numerical score advantage at 42, 62, and 82 minutes in these categories, the advantage was statistically insignificant. The G30 formula scored lower than the G18 for tartness at 62 minutes.
- W scored lower than G18 for overall acceptance and flavor at all evaluation times. It also scored lower for aftertaste than G18 at the initial evaluation (32 minutes).
- The G18 formula was rated less salty than ideal at all evaluation times. G18 was rated more sour than ideal at 32, 42, and 82 minutes. There were no differences in perceived saltiness between G18 and G30. Compared to G18, G30 was less sour at 42 and 62 minutes, sweeter at 82 minutes, and less off at 62 minutes. The G30 formula was rated less salty than ideal at all evaluation times.
- This study compared the electrolyte formulations shown below to determine how rapidly fluid is lost and how much is retained up to three hours after exercise-induced dehydration that is followed by replacement of total fluid loss from sweat.
[Na], [K], [Cl], Osmolality, CODE mEq/L mEq/L mEq/L mOsm/kg G18 18.4 2.8 11.5 307 G25 25.1 9.7 33.9 339 G30 29.7 2.7 20.2 325 - In addition, the study was to determine the effects of lowering the sodium to 25 mEq/L while increasing the sum of the total extracellular ions—sodium and chloride—to levels above the G30 formulation.
- For this study, subjects were divided into two groups to assess the effect of controlling pre-experiment diet (over 24 hours) on physiology measures. As in the previous phase, subjects were fed a standard diet for dinner the evening prior to testing, and for breakfast and lunch the day of the test.
- For the controlled dietary group the total caloric intake was 2200 calories and approximately 2400 mg of sodium. Subjects who weighed over 150 pounds had slightly higher (+200) calories and sodium intake (+100 mg). All subjects were given a written copy of the dietary and exercise guidelines, and subjects were instructed not to substitute foods, but additional liquids (within guidelines) were permitted until 3 hours prior to the experiment.
- All subjects were required to follow the diet and exercise guidelines prior to participating. In order to assure pre-experiment hydration, each subject was required to ingest adequate fluid. Pre-experiment hydration was further assured by checking the conductivity of the pre-experiment urine sample collected immediately prior to the testing. Subjects whose first experimental urine sample had a conductivity that exceeded 21 millisiemens-cm were dismissed from the experiment and required to reschedule.
- Thirty-six subjects (31 men, 5 women) completed the study. On separate occasions, they exercised in the heat (80° F./40% RH) for 1.5 hours and dehydrated on average 1.8±0.6% and replaced 100% of their sweat loss with one of three levels of sodium (18, 25, or 30 mEq/L). Average sweat rate for the entire group across all trials was 0.885±0.32 liters per hour.
- Women were included in this study and the effects associated with changes in the menstrual cycle, fluid balance, and hormonal influence were controlled by studying the women when they were in the same phase of their cycle for all testing. This study was designed to determine the rate of fluid loss after ingesting beverages with varying mineral content following exercise-induced dehydration in the heat. The testing protocol and sequence are shown in
FIG. 21 . - Subjects were then weighed for a baseline bodyweight, and a sensory (GI Symptoms, Energy levels) questionnaire was completed. Subjects cycled in a warm environment (80° F., 40% RH) for 1.5 hours to elicit an exercise-induced dehydration near two percent of initial bodyweight. A previously determined workload was used to set an exercise intensity equivalent to 70-75% of maximal heart rate as determined by earlier maximal treadmill tests. Heart rate was monitored every 10 minutes using a telemetered HR monitor (Polar) and to assure sufficient intensity of exercise.
- At 1-hour, subjects were allowed a 5-minute break from cycling. At the end of the exercise-dehydration period, subjects were given a sensory form, and subjects were weighed again to determine final sweat loss. A post-exercise urine sample was collected immediately following the post-exercise body weight. The subjects were then seated in a reclined position for the remainder of the recovery period.
- Beverages were mixed prior to the experiment using 1-liter and 2-liter volumetric flasks and beverage grade water that did not contribute any electrolytes to the beverage. Beverages were served chilled from the refrigerator (approximately 40° F.), although the last two beverages were likely to be closer to ambient temperature by the time they were served to the subjects. Beverages were distributed according to a rehydration scheme that involved dosing the volume according to specific times. At 30 minutes into recovery, subjects received their first of six cups of beverage, followed by another cup every ten minutes. Total beverage volume was equivalent to total sweat loss, and was portioned such that subjects received 50% of the total volume in the first 20 minutes and the remaining 50% in 12.5% portions every ten minutes for the remaining 40 minutes. All fluid was consumed within an hour.
- Every 30 minutes during the 3.5-hour recovery, subjects were given a sensory form to complete, and urine samples were taken. Urine volume was determined by weight, and urine conductivity was immediately determined using a conductivity meter (WTW LF 340, model 19706-20). Urine was then aliquoted and stored at −20° F. until later analysis for [Na+] and [K+]. Urine [Na+] and [K+] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed.
- Data were analyzed using a computer statistics package (SPSS, v.10). Descriptive statistics and summary tables were generated for all dependent measures. Repeated measures ANOVA was used to test for main effects of treatment and where appropriate time dependent effects. Where main effects existed, a Duncan post-hoc test was used to determine statistical differences among means. Comparisons were tested with an alpha level equal to 0.05. Effect size estimates were made for comparisons of total urine loss between treatments.
- Study results are broken into separate groups. Results for the entire study group are shown first, followed by diet-controlled men, then by men only.
- Thirty-six subjects (N=36) completed all three trials. Four subjects either withdrew or were dismissed from the study due to illness, or inability to tolerate dietary restriction (caffeine withdrawal). Average loss of bodyweight during the exercise-induced dehydration was 1.8±0.6% over 1.5 hours. The subjects exhibited an average sweat rate of 0.89±0.32 liters/hr and lost an average of 1.3±0.5 liters of sweat during the dehydration period. Dehydration and sweat loss did not differ across treatments.
- The percent of ingested fluid retained differed significantly. G25 resulted in significantly more fluid retention (79.6%) compared to G18 (73.5%) and G30 (75.1%). When corrected for bodyweight (ml/kg) or examined in absolute terms (kilograms), the significant effect disappears. Contrary to results from Project FR-1
phases - Average total urine excreted during 2.5 hours of recovery was significantly less with G25 (0.252 L) than with G30 (0.322 L) and G18 (0.349 L). When corrected for bodyweight (e.g. ml/kg) the effect holds significant. Effect size estimates were made for the following comparisons for total urine loss: 18 vs. 25, ES=0.57 (medium effect size, significant); 18 vs. 30, ES=0.14 (no effect); 25 vs. 30, ES=0.49 (medium effect size, non-significant). Formulations began to differentiate at 150 minutes of recovery (1 hr after drinking was complete).
- Absolute change in body weight from baseline to end of recovery was −0.72, −0.62, and −0.71 kilograms for G18, G25, and G30, respectively. Expressed as a percentage of initial weight, these values were −0.98%, −0.84%, and −0.96% for G18, G25, and G30, respectively. Both absolute and percentage variables were significantly different for G25 versus G18 and G30 when subject variability was controlled for in the statistical model. G18 did not differ from G30.
- Mean Specific Electrical Conductivity (SEC) for baseline urine samples ranged between 17-18 mS-cm across all treatments. Average baseline urine volumes were 111.4, 117.1, and 121.8 grams for G18, G25, and G30 respectively. On average, this indicates that subjects started the protocol in moderately hydrated state.
- Average urine volumes excreted, independent of time, were 58.2, 42.0, and 53.7 grams, for G18, G25, and G30, respectively. Interestingly, mean SEC independent of time was 13.1, 16, and 14.5 mS-cm for G18, G25, and G30, respectively.
- To examine any sensory (taste) and perceptual changes associated with the various treatments, the subjects rated a number of psychophysiological characteristics on several different scales (e.g. categorical, 100 pt) throughout the experimental period. Both a GI distress and a Sensory (taste) form were used periodically throughout the test to examine subjects feelings.
- There were no differences across products for any ratings on the GI form. This included aspects of: energy, alertness, thirst, hunger, stomach fullness, nausea, and urge to urinate.
- Some sensory attributes of the different products were rated significantly different. Overall acceptance across products showed similar scores when the beverages were first received, but differed significantly at the last beverage (82 min). G25 had significantly less appeal (6.4) compared to G18 (7.0), but did not differ from G30 (6.8). Liking of saltiness was less for G25 compared to G18 and G30 for all time points tested. This may indicate a negative attribute of the additional chloride in G25. While not significant, G25 was perceived directionally more salty than G18 or G30. Liking of flavor scores were similar across time and product. GI attributes varied over time, consistent with the time of day and protocol. There were no remarkable differences to report. Taste and flavor attributes of the different products did not vary over the short duration (1-hr) that they were consumed.
- Further probing of the data was completed with subgroups as defined below. This was done in response to learning that a variety of factors had significant impact on the outcome. The following results are from analyses in subgroups of: Diet Controlled (Men Only); Men Only. Due to the few women participants, it worked out such that there were only men in the group that tested with a controlled diet.
- Seventeen (n=17) of the 36 subjects were stratified into the group of men that ate a controlled diet. In this subset of participants, average loss of bodyweight during the exercise-induced dehydration was 1.8±0.5% over 1.5 hours. These subjects exhibited an average sweat rate of 0.95±0.28 liters/hr and lost an average of 1.4±0.4 liters of sweat during the dehydration period. Dehydration and sweat loss did not differ across treatments.
- The percent of ingested fluid retained in this subgroup did not differ significantly (p=0.089). Directionally, G25 resulted in significantly more fluid retention (80.7%) compared to G18 (74.9%) and G30 (74.2%), but the differences were not great enough for this subset to reach statistical significance.
- Total urine loss varied by sodium level (p=0.029) Average total urine excreted during 2.5 hours of recovery was significantly less with G25 (0.255 L) than with G30 (0.374 L) and G18 (0.367 L). Effect sizes are as follows: 18 vs. 25, ES=0.58 (medium effect size); 18 vs. 30, ES=0.04 (no effect); 25 vs. 30, ES=0.71 (large effect size).
- Absolute change in body weight from baseline to end of recovery was −0.70, −0.59, and −0.75 kilograms for G18, G25, and G30, respectively (p=0.015). Expressed as a percentage of initial weight, these values were −0.94%, −0.79%, and −0.99% for G18, G25, and G30, respectively. Both absolute and percentage variables were significantly different for G25 versus G18 and G30 when subject variability was controlled for in the statistical model. G18 did not differ from G30.
- Thirty-one (n=31) of the 36 subjects were stratified into a group of men, independent of diet control group. In this subset of the entire group, the largest differences were noted, perhaps due to the large sample size (n=31) and statistical removal of the effect imposed by the small group of women tested. In this subset of participants, average loss of bodyweight during the exercise-induced dehydration was 1.9±0.6% over 1.5 hours. These subjects exhibited an average sweat rate of 0.94±0.31 liters/hr and lost an average of 1.4±0.5 liters of sweat during the dehydration period. Dehydration and sweat loss did not differ across treatments.
- The percent of ingested fluid retained differed significantly (p=0.016). G25 resulted in significantly more fluid retention (79.6%) compared to G18 (73.1%) and G30 (75.0%). The treatment effects became apparent at 150 minutes into the recovery segment [see
FIG. 22 ]. When corrected for bodyweight (ml/kg) or examined in absolute terms (kilograms), the significant effect disappears. - Average total urine excreted during 2.5 hours of recovery was significantly (p=0.009) less with G25 (0.267 L) than with G30 (0.346 L) and G18 (0.374 L). When corrected for bodyweight (e.g. m l/kg) the effect holds significant (p=0.005). Products began to differentiate at 150 minutes of recovery (1 hr after drinking was complete) [see
FIG. 23 ]. Effect size estimates are as follows: 18 vs. 25, ES=0.62 (medium effect size); 18 vs. 30, ES=0.15 (no effect); 25 vs. 30, ES=0.56 (medium effect size). - Absolute change in body weight from baseline to end of recovery was −0.76, −0.65, and −0.75 kilograms for G18, G25, and G30, respectively. Expressed as a percentage of initial weight, these values were −1.01%, −0.85%, and −0.99% for G18, G25, and G30, respectively. Both absolute (p=0.006) and percentage variables (p=0.010) were significantly different for G25 versus G18 and G30 when subject variability was controlled for in the statistical model. G18 did not differ from G30.
- The data are consistent with findings reported for the entire group. There are sporadic differences between beverages. The most notable effect observed was that ‘liking of saltiness’ was lowest and perceived saltiness was highest for G25 compared to G18. This effect was fairly consistent throughout the entire drinking period. There were no remarkable GI related effects observed across the beverages in this subgroup of men.
- This study was similar to the previous one. This time however, the sum of chloride and sodium ions in 25 mEq/L formulation was more similar to the sum of sodium and chloride in the G30 formulation and the 18 mEq/L formulation was not included. Specific formulations of the electrolytes are shown below.
[Na+], [K+], [Cl−], [Mg++], Osm Code mEq/L* mEq/L* mEq/L mEq/L (mOsm/kg)* G25 25.1 9.9 30 2 339 G30 29.5 2.8 20 0 334 - These formulations were compared for their effects on fluid loss and fluid retention. In addition, their sensory qualities were measured.
- Subjects reported to the laboratory and workloads were set for the bicycle and treadmill sufficient to produce intensities between 75-80% of their measured maximum heart rates (determined from annual stress tests). In addition, body weights were obtained prior to and following the orientation exercise session in order to predict sweat rates.
- Subjects were provided with standardized meals to ensure consistent sodium intake (˜2700 mg) prior to each of the two trials. Meals included dinner (evening prior to testing), and breakfast and lunch on the day of testing. Subjects were given the option of eating all or a portion of the food provided but were instructed to record which foods and amount left uneaten. These items were then withheld from the food bags for the next trial. In addition, they were given bottled water to drink the evening before and during the testing day to ensure adequate hydration. Subjects were asked to refrain from caffeine and alcohol use for 24-hours prior to the experiment.
- Subjects included trained men (ages 25-49). Seventeen completed both trials. One subject withdrew from testing due to illness. Subjects reported to the facility following a 3-hour fast (drinking and eating), a urine sample was taken to assess pre-exercise hydration status and were weighed.
- After completing a pre-exercise survey (GI ratings), they began exercise. The exercise session consisted of alternate cycling/running at 15-minute intervals at 75-80% maximum heart rate for a total of 60 minutes. Heart rates were taken at 15-minute intervals to ensure subjects maintained adequate intensity. Subjects refrained from drinking during the entire exercise period in an effort to produce 1.5-2% dehydration.
- Following exercise, subjects were weighed and a post-exercise urine sample was taken. A 3.5 hour recovery period followed. At 30 minutes into recovery, subjects were given their first beverage equivalent to 25% of their total sweat loss (determined by subtracting post-exercise weight from pre-exercise weight) and a sensory questionnaire. A questionnaire and beverage (25% of losses) was given again at 40 minutes into recovery.
Drink # 3, equivalent to 12.5% of sweat loss, was given at 50 minutes. At 58 minutes, subjects were given a GI scale to assess gastro-intestinal responses and were escorted to the restroom for a urine sample. They filled out a sensory form when they returned and were given their 4th drink (12.5%). Drink #5 (12.5%) was given at 70 minutes, drink #6 (12.5%), their final drink, and a sensory questionnaire was given at 80 minutes. At 90, 120, 180 and 240 minutes, GI scales were given and urine samples were collected. After the final urine sample, subjects were taken back upstairs for a final nude body weight. They were given meals for the next trial (if necessary) and were released. - Urine volume was determined by weight and urine specific gravity was measured (A 300 Clinical Refractometer). Urine was the transferred into 4-ml cryovials for further analysis. Urine [Na+] and [K+] were determined using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- Each subject was given food equaling 2,428 kcal and 2,694 mg of sodium. They were instructed to eat as much or as little as they desired, but to remain consistent between treatments. Most subjects ate all the food provided to them, however, intakes ranged from 1,851-2,428 kcal and 1,831-2694 mg sodium. None of the subjects ate food other than what was provided.
- The 60-minute exercise session resulted in similar levels of dehydration between treatments (1.75±0.29% and 1.78±0.33% for G25 and G30, respectively). In addition, sweat rates between treatments were essentially the same (1.39±0.32 L/hour for G25 and 1.40±0.32 L/hour for G30).
- Total fluid intake between treatments was 1.39±0.32 L for the G25 treatment and 1.40±0.32 L for the G30 treatment matching the values for total sweat losses.
- Total cumulative fluid output was not different between treatments (0.35±0.13 L G25, 0.35±0.15 L G30). Additionally, urine output at each data collection point was not significantly different between treatments (See below).
60 90 120 180 240 Treatment minutes minutes minutes minutes minutes G25 35.28 ± 19.65 73.39 ± 25.04 159.68 ± 59.36 276.23 ± 104.25 345.80 ± 130.13 G30 37.55 ± 22.40 70.58 ± 37.21 153.47 ± 86.35 272.45 ± 138.93 352.41 ± 152.08
Each time point represents the number of minutes into recovery. - Fluid retention, or the amount of fluid consumed that was not excreted as urine, was not different between treatments. This was calculated in both relative terms (ml/kg) and as a percentage calculated as (fluid in−fluid out)/fluid in. Relative fluid retention was 13.11±3.04 ml/kg and 13.32±3.52 ml/kg for G25 and G30, respectively. Percent fluid retained was also the same for both treatments (74.29±9.76% for G25 and 74.20±10.14% for G30). Predictably, percent fluid retention across time was also the same for each treatment (see below).
60 90 120 180 240 Treatment minutes minutes minutes minutes minutes G25 95.86 ± 2.10 94.54 ± 1.69 88.00 ± 5.03 87.74 ± 8.84 74.29 ± 9.76 G30 95.31 ± 3.44 94.56 ± 3.22 88.25 ± 7.45 89.02 ± 10.05 74.20 ± 10.14 - Subjects were asked to rate a number of psychophysiological characteristics on several different scales (e.g. categorical, 100 pt) throughout the experiment period to examine any sensory and perceptual changes associated with the treatments. A GI distress and a Sensory (taste) form were used periodically throughout the test to examine subjects' perceptions. There were no important differences between the two beverages on ratings of perceived physiological and psychological well-being. Differences in beverage acceptance are as follows. The G30 scored significantly higher than G25 in acceptance and liking of sweetness, tartness, flavor, and saltiness, and had a large effect size (0.7) advantage in aftertaste at 32 minutes. See
FIG. 24 for overall acceptance. At 42 minutes, G30 was significantly more acceptable than G25 for aftertaste, and had a medium effects size advantage in tartness. G25 was more tart than G30 at all times. SeeFIG. 25 . G25 was significantly more salty than G30 at 32 minutes and was not different at all other time points. G25 perception of saltiness was initially rated as ideal but became decreasingly less than ideal at all other time points. G30 was less than ideal saltiness at all time points. SeeFIG. 26 . - This study determined the effects on rehydration of modifying the G30 formula to include calcium and magnesium and a higher level of potassium. As in Example 5, the G18 and POWERade® formulations were included along with two modified versions of G30 labeled K10 and K20. As in all Examples, subjects as well as the experimenters were blinded to the beverage treatments. Specific formulations of the electrolytes are shown below.
[Na], [K], [Cl], [Ca], [Mg], Code mEq/L mEq/L mEq/L mEq/L mEq/ L P 5 3 ˜6 0 0 G18 18 3 ˜12 0 0 K10 30 10 ˜20 1 3 K20 30 20 ˜20 1 ˜6 - Subjects were provided with standardized meals to ensure consistent sodium intake (˜3000 mg) prior to each of the experimental trials. Meals included dinner (evening prior to experiment), and breakfast and lunch on the day of testing. Subjects were given the option of eating all or a portion of the food provided. They were also instructed to record which foods were left uneaten. These items were then withheld from the food bags for the next trial. In addition, they were given bottled water to drink the evening before (500 ml) and during the testing day (1000 ml) to ensure adequate hydration. Subjects were asked to refrain from caffeine and alcohol use for 24-hours prior to the experiment.
- Subjects included trained men ages 30-50 y. Seventeen completed both trials. Testing followed a 3-hour fast (drinking and eating). Body weights were obtained prior to and following the exercise session. In addition, urine samples were taken to assess pre-exercise hydration status. Subjects also completed a pre-exercise survey (GI ratings).
- The exercise session consisted of 30-minutes each on the cross trainer, stationary bike and treadmill at 70-75% maximum heart rate for a total of 90 minutes. Heart rates were taken at 15-minute intervals to ensure subjects maintained adequate intensity. Subjects refrained from drinking during the entire exercise period in an effort to produce 2-2.5% dehydration.
- Following exercise, subjects were weighed and gave a post-exercise urine sample, followed by a 3.5 hour recovery period. Thirty minutes into recovery, subjects were given their first beverage equivalent to 25% of their total sweat loss (determined by subtracting post-exercise weight from pre-exercise weight) and a sensory questionnaire. A questionnaire and beverage (25% of losses) was given again at 40 minutes into recovery. The third aliquot of beverage, equivalent to 12.5% of sweat loss, was given at 50 minutes. At 58 minutes, subjects were given a GI scale to assess gastro-intestinal responses and urine samples were taken. Subjects then filled out a sensory form and were given their fourth aliquot (12.5%). The fifth aliquot (12.5%) was given at 70 minutes, and the sixth and final aliquot (12.5%) and a sensory questionnaire was given at 80 minutes. At 90, 120, 180 and 240 minutes, GI scales were given and urine samples were collected. After the final urine sample, subjects were weighed for a final nude body weight.
- Urine volume was determined by weight and urine specific gravity was measured (A 300 Clinical Refractometer). Urine was then transferred into 4-ml cryovials for subsequent analysis of sodium and potassium concentrations using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- SPSS version 11.0 was used to analyze the data. ANOVA using a general linear model was used to determine differences between mean values. Data is reported as the mean+the standard deviation.
- Exercise resulted in similar levels of dehydration between treatments (2.56±0.56%, 2.53±0.55%, 2.57±0.51%, and 2.50±0.44% for P, G18, K10, and K20, respectively). In addition, sweat rates between treatments were essentially the same (1.29±0.29 L/hr, 1.27±0.32 L/hr, 1.29±0.31, 1.26±0.26 L/hr P, G18, K10, and K20, respectively).
- Total fluid intake between treatments was also similar between trials at 1.92±0.44 L for P, 1.92±0.48 L for G18, 1.93±0.46 L for K10 and 1.87±0.39 L for K20. The volume administered varied slightly in mean value because of the modest variability in sweat rate and the protocol of replacing 100% of the total sweat losses.
- Final body weight as a percentage of initial body weight did not differ between treatments. Subjects returned to 98.68±0.53% of initial body weight following the P trial, 98.85±0.27% following G18, and 98.84±0.47% and 98.89±0.33% for K10 and K20, respectively.
- Total cumulative fluid output did not reach statistical significance between treatments (
FIG. 32 ). Cumulative fluid output was 501.03±303.93 ml, 444.63±210.19 ml, 434.05±276.62 ml, and 353.08±183.47 ml, for P, G18, K10, and K20, respectively. Urine output at each data collection point was not significantly different between treatments fortime points time points - Fluid retention, or the amount of fluid consumed that was not excreted as urine, was not different between treatments. This was calculated in both relative terms (ml/kg) and as a percentage calculated as (fluid in−fluid out)/fluid in. Relative fluid retention was 18.62±7.81 ml/kg, 19.40±5.65 ml/kg, 19.66±5.99 ml/kg, and 20.17±5.07 ml/kg for P, G18, K10 and K20, respectively. No statistical differences in percent fluid retained were found between treatments (73.70±13.85% for P, 75.74±12.56% for G18, 76.39±14.23% for K10, and 80.53±9.69% for G30). However, an effect size of 0.58 was found between K20 and P (p=0.09). See
FIG. 27 . - Total urine production during the recovery period was not significantly different between treatments. However, an effect size of 0.61 was found between the K20 treatment and P (p=0.07). Additionally, at
time points - Mean urine osmolality was different between treatments. P resulted in the lowest urine osmolality (379.70±222.96 mOsm) and was not different than G18 (415.51±217.89 mOsm) or K10 (413.53±232.11 mOsm) but was significantly different than K20 (462.42±226.70 mOsm). K20 was not different than K10 or G18. See
FIG. 28 . - Average urine sodium concentration was significantly greater for the K20 trial (52.59±31.38 mEq/L) compared to P (40.74±28.58 mEq/L) and G18 (43.81±27.98 mEq/L). K10 (47.87±35.18 mEq/L) was not significantly different than any of the trials. Total sodium excretion (urine volume×urine sodium concentration) was not different across trials (P=16.62±8.32; G18=17.68±10.80; K10=19.46±10.17; K20=20.27±8.77). See
FIG. 29 . - The average urine potassium concentration was significantly higher for the K20 trial (58.44±32.59 mEq/L) compared to P (42.24±40.19 mEq/L). G18 (47.26±37.05) and K10 (51.99±39.33) were not different from any of the treatments.
- Subjects began each exercise session well hydrated (USG=1.008±0.005). Urine specific gravity did not differ between treatments prior to exercise. Average USG for W treatment was significantly less than P at
minute 120. Additionally W was less that G18 and G30 atminute 180. USG for P was significantly less than G30 atminutes FIG. 30 . - There were no differences among the beverages for overall acceptance based on the sensory surveys taken at any of the four evaluation times. Formula K20 and Powerade scored significantly higher for saltiness acceptance than G18 at 32 minutes. This was the only case where a significant difference was found across products at any time. P showed a significant decline in overall acceptance with time. While P initially, had the numerically highest overall acceptance score, it was directionally the least acceptable overall. P also had directional decreases over time for all acceptance scales except aftertaste. Formula G18 had relatively low initial (32 minute) scores for liking of aftertaste, saltiness, and tartness. These scores rebounded into a more typical range at later times.
- There were essentially no significant differences across the beverages for gastrointestinal distress. At 60 minutes, the K20 was rated as contributing to significantly more nausea than G18 and K10. This, however, was directionally consistent with the results at 30 minutes, since feelings of nausea are common following the exercise protocol and just before the beverages are administered. Numerous differences across time were observed. These were consistent with what would be expected from the protocol. No unusual states of gastric distress were observed.
- Besides varying the potassium levels and adding calcium and magnesium as done in the previous Example, the levels of all the electrolytes except for sodium were altered to determine their combined effects on rehydration. Three formulations were tested in addition to G30. All formulations consisted of 6% carbohydrate, 30 mEq/L of sodium and 0.103% flavoring. As in other Examples, the carbohydrate was a blend of sucrose (˜3%), glucose (˜1.7%) and fructose (˜1.3%). Specific formulations of the electrolytes are shown below.
[Na], [K], [Cl], [Ca], [Mg], Code mEq/L mEq/L mEq/L mEq/L mEq/ L G30 30 3 ˜17 0 0 K5 30 5 ˜20 3 3 K10 30 10 ˜17 3 3 K20 30 20 ˜17 1 3 - The subjects included trained men (ages 19-50 y) Eighteen completed all four trials. Nearly identical to the previously examples, subjects were provided with standardized meals each day prior to the studies. Testing followed a 3-hour fast (drinking and eating). Prior to beginning exercise urine samples were obtained to assess pre-exercise hydration status and subjects were weighed.
- The subjects exercised for a total of 90 minutes without consuming any fluids. The session consisted of 30-minutes each on the cross trainer, stationary bike and treadmill at 70-75% maximum heart rate for a total of 90 minutes. The exercise induced 2.5-3% dehydration in the subjects. After exercise, subjects were weighed and urine samples obtained, followed by a 3.5 hour recovery period.
- Thirty minutes into recovery, subjects were given their first beverage equivalent to 25% of their total sweat loss (determined by subtracting post-exercise weight from pre-exercise weight) and a sensory questionnaire. A questionnaire and beverage (25% of losses) was given again at 40 minutes into recovery. The third aliquot of beverage, equivalent to 12.5% of sweat loss, was given at 50 minutes. At 58 minutes, subjects were given a GI scale to assess gastro-intestinal responses and sensation, and were escorted to the restroom for a urine sample. They then filled out a sensory form and were given a fourth aliquot (12.5%). The fifth aliquot (12.5%) was given at 70 minutes and the sixth and final aliquot (12.5%) and a sensory questionnaire was given at 80 minutes. At 90, 120, 180 and 240 minutes, subjects were again assessed for GI responses and had urine samples collected. After the final urine sample, subjects were weighed for a final nude body weight.
- All urine samples were collected in disposable specimen containers, were weighed to determine the urine excretion volume and were measured for urine specific gravity (A 300 Clinical Refractometer). Urine was then transferred into 4-ml cryovials for subsequent analysis of sodium and potassium concentrations using flame photometry (IL943 Automatic Flame Photometer) after centrifuging for 15 minutes to remove any insoluble particles from the sample to be analyzed. Osmolality was measured for each sample (Fiske 2400 Osmometer).
- Total sweat rates were lower than in past studies due to the difficulty in heating the room during the winter months. Nevertheless, sweat rates were sufficient to produce 2-2.5% dehydration. The exercise session resulted in similar levels of dehydration between treatments (2.10±0.50%, 2.18±0.51%, 2.14±0.53%, and 2.15±0.50% for G30, K5, K10 and K20, respectively). In addition, sweat losses between treatments were essentially the same (1.12±0.25 L/hr, 1.16±0.27 L/hr, 1.14±0.26, 1.14±0.25 L G30, K5, K10, and K20, respectively).
- Total fluid intake between treatments was also similar between trials at 1.68±0.38 L for G30, 1.75±0.40 L for K5, 1.71±0.39 L for K10 and 1.72±0.37 L for K20. The volume administered varied slightly in mean value because of the modest variability in sweat rate and the protocol of replacing 100% of the total sweat losses.
- Final body weight as a percentage of initial body weight did not differ between treatments. Subjects returned to 98.96±0.21% of initial body weight following the G30 trial, 98.96±0.29% following K5, and 98.90±0.37% and 98.95±0.31% for K10 and K20, respectively.
- Total cumulative fluid output was not different between treatments. Cumulative fluid output was 353.99±161.73 ml, 369.13±167.35 ml, 380.93±240.35 ml, and 386.93±205.07 ml, for G30, K5, K10, and K20, respectively. Urine output at each data collection point was also not different between treatments for any time point. The percent of fluid retained was not different between formulae (78.17±11.45% for G30, 76.60±16.84% for K5, 78.28±10.83% for K10, and 76.62±12.87% for K2). See
FIG. 31 . Relative to body weight, fluid retention did not differ either: 16.59±5.09 ml/kg, 17.29±5.04 ml/kg, 16.69±6.06 ml/kg, and 16.66±5.31 ml/kg for G30, K5, K10 and K20, respectively. - Mean urine osmolality was also not different between treatments (G30=488±228 mOsm, K5=484±250 mOsm, K10=456±215 mOsm, K20=492±215 mOsm) nor was urine osmolality different at any time during recovery. See
FIG. 32 . Subjects began each exercise session well hydrated (USG=1.008±0.005). Urine specific gravity (USG) did not differ between treatments prior to exercise or at any time point during recovery. Similarly, the change in urine specific gravity did not different between treatments SeeFIG. 33 . - Average urine sodium concentration was not different across trials (54.73±34.53 mEq/L, 60.34±38.44 mEq/L, 53.31±32.56 mEq/L, 60.99±35.99 mEq/L for G30, K5, K10, and K20, respectively). Total sodium excretion (urine volume×urine sodium concentration) was also not different across trials (G30=20.61±10.12 mEq; K5=24.66±12.86 mEq; K10=21.65±10.28 mEq; K20=24.97±12.49 mEq).
- The average urine potassium concentration was significantly higher for the K20 trial (56.59±33.43 mEq/L) compared to G30 (49.79±32.87 mEq/L), K5 (47.24±36.11) and K10 (46.87±28.96). The latter three were not different from each other. See
FIG. 34 . - Overall acceptance as determined from the sensory ratings during the rehydration period showed no differences between the beverages. See
FIG. 35 . - Although this invention has been described in terms of certain preferred compositions and certain discrete methods, other embodiments that may be apparent to those skilled in the art are also within the scope of this invention. Accordingly, the scope of the invention is intended to be determined by reference to the appended claims.
Claims (184)
1. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 8 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
2. The beverage of claim 1 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
3. The beverage of claim 1 wherein said carbohydrate source is a mixture of a minimum of three of the following types of carbohydrates: sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate with the amount of fructose, if selected, always less than the total amount of glucose from all sources.
4. The beverage of claim 1 wherein said sodium comprises from about 30 to about 100 mEq/L.
5. The beverage of claim 1 wherein said sodium comprises from about 30 to about 60 mEq/L.
6. The beverage of claim 1 wherein said sodium comprises from about 33 to about 40 mEq/L.
7. The beverage of claim 4 wherein said sodium comprises from about 10 to about 50 mEq/L of sodium chloride and from about 10 to about 50 mEq/L of sodium citrate.
8. The beverage of claim 5 wherein said sodium comprises from about 10 to about 30 mEq/L of sodium chloride and from about 10 to about 30 mEq/L of sodium citrate.
9. The beverage of claim 1 wherein said chloride comprises from about 11 to about 18 mEq/L.
10. The beverage of claim 1 wherein said potassium comprises from about 8 to about 20 mEq/L.
11. The beverage of claim 1 further comprising calcium from about 1 to about 6 mEq/L.
12. The beverage of claim 1 further comprising magnesium from about 1 to about 6 mEq/L.
13. The beverage of claim 1 further comprising a flavoring from about 0 to about 0.4% by weight.
14. The beverage of claim 1 further comprising a clouding agent from about 0 to about 100 parts per million.
15. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 8 mEq/L of potassium;
(e) from about 1 to about 6 mEq/L of calcium;
(f) from about 1 to about 6 mEq/L of magnesium;
(g) from about 0 to about 0.4% by weight of a flavoring agent;
(h) from about 0 to about 100 parts per million of a clouding agent; and
(i) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
16. The beverage of claim 15 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
17. The beverage of claim 15 wherein said sodium comprises from about 30 to about 100 mEq/L.
18. The beverage of claim 15 wherein said sodium comprises from about 30 to about 60 mEq/L.
19. The beverage of claim 15 wherein said sodium comprises from about 33 to about 40 mEq/L.
20. The beverage of claim 17 wherein said sodium comprises from about 10 to about 50 mEq/L of sodium chloride and from about 10 to about 50 mEq/L of sodium citrate.
21. The beverage of claim 18 wherein said sodium comprises from about 10 to about 30 mEq/L of sodium chloride and from about 10 to about 30 mEq/L of sodium citrate.
22. The beverage of claim 15 wherein said chloride comprises from about 11 to about 18 mEq/L.
23. The beverage of claim 15 wherein said potassium comprises from about 8 to about 20 mEq/L.
24. The beverage of claim 15 wherein said calcium comprises from about 1 to about 3 mEq/L.
25. The beverage of claim 15 wherein said magnesium comprises from about 1 to about 3 mEq/L.
26. A beverage composition comprising:
(a) from about 4.5 to about 6.5% by weight of carbohydrates;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 8 mEq/L of potassium;
(e) from about 1 to about 3 mEq/L of calcium;
(f) from about 1 to about 3 mEq/L of magnesium;
(g) from about 0 to about 0.2% by weight of a flavoring agent;
(h) from about 0 to about 50 parts per million of a clouding agent; and
(i) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
27. The beverage of claim 26 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
28. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 30 to about 40 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 8 mEq/L of potassium;
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
29. The beverage of claim 28 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
30. The beverage of claim 28 wherein said sodium comprises from about 10 to about 20 mEq/L of sodium chloride and from about 10 to about 20 mEq/L of sodium citrate.
31. The beverage of claim 28 wherein said chloride comprises from about 11 to about 18 mEq/L.
32. The beverage of claim 28 wherein said potassium comprises from about 8 to about 20 mEq/L.
33. The beverage of claim 28 further comprising calcium from about 1 to about 6 mEq/L.
34. The beverage of claim 28 further comprising magnesium from about 1 to about 6 mEq/L.
35. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) from about 8 to about 20 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
36. The beverage of claim 35 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
37. The beverage of claim 35 wherein said sodium comprises from about 30 to about 60 mEq/L.
38. The beverage of claim 35 wherein said sodium comprises from about 30 to about 40 mEq/L.
39. The beverage of claim 37 wherein said sodium comprises from about 10 to about 30 mEq/L of sodium chloride and from about 10 to about 30 mEq/L of sodium citrate.
40. The beverage of claim 38 wherein said sodium comprises from about 10 to about 20 mEq/L of sodium chloride and from about 10 to about 20 mEq/L of sodium citrate.
41. The beverage of claim 35 wherein said chloride comprises from about 11 to about 18 mEq/L.
42. The beverage of claim 35 wherein said potassium comprises from about 10 to about 19 mEq/L.
43. The beverage of claim 35 further comprising calcium from about 1 to about 6 mEq/L.
44. The beverage of claim 35 further comprising magnesium from about 1 to about 6 mEq/L.
45. A beverage composition comprising:
(a) from about 4.5 to about 6.5% by weight of carbohydrates;
(b) from about 30 to about 40 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) from at about 8 to about 20 mEq/L of potassium;
(e) from about 1 to about 3 mEq/L of calcium;
(f) from about 1 to about 3 mEq/L of magnesium;
(g) from about 0 to about 0.2% by weight of a flavoring agent;
(h) from about 0 to about 50 parts per million of a clouding agent; and
(i) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
46. The beverage of claim 45 further comprising citric acid.
47. The beverage of claim 46 having a pH of from about 2.5 to about 4.5.
48. The beverage of claim 47 wherein said beverage is isotonic.
49. The beverage of claim 45 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
50. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment; and;
(c) at least about 8 mEq/L of potassium; and
(d) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
51. The beverage of claim 50 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
52. The beverage of claim 50 further comprising from about 1 to about 6 mEq/L of calcium.
53. The beverage of claim 50 further comprising from about 1 to about 6 mEq/L of magnesium.
54. The beverage of claim 50 wherein said ions which favor the filling of the extracellular fluid compartment comprise sodium and chloride ions.
55. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment;
(c) at least about 8 mEq/L of potassium;
(d) from about 1 to about 6 mEq/L of calcium;
(e) from about 1 to about 6 mEq/L of magnesium;
(f) from about 0 to about 0.4% by weight of a flavoring agent;
(g) from about 0 to about 100 parts per million of a clouding agent; and
(h) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
56. The beverage of claim 55 wherein said ions which favor the filling of the extracellular fluid compartment comprise sodium and chloride ions.
57. The beverage of claim 55 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 42 to about 70 mEq/L.
58. The beverage of claim 55 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 46 to about 60 mEq/L.
59. The beverage of claim 57 wherein said potassium comprises from about 8 to about 20 mEq/L.
60. The beverage of claim 57 wherein said calcium comprises from about 1 to about 3 mEq/L.
61. The beverage of claim 57 wherein said magnesium comprises from about 1 to about 3 mEq/L.
62. A beverage composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment;
(c) from about 8 to about 20 mEq/L of potassium;
(d) from about 1 to about 6 mEq/L of calcium;
(e) from about 1 to about 6 mEq/L of magnesium;
(f) from about 0 to about 0.4% by weight of a flavoring agent;
(g) from about 0 to about 100 parts per million of a clouding agent; and
(h) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
63. The beverage of claim 62 wherein said ions which favor the filling of the extracellular fluid compartment comprise sodium and chloride ions.
64. The beverage of claim 62 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 42 to about 70 mEq/L.
65. The beverage of claim 62 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 46 to about 60 mEq/L.
66. The beverage of claim 62 wherein said potassium comprises from about 10 to about 19 mEq/L.
67. The beverage of claim 62 wherein said calcium comprises from about 1 to about 3 mEq/L.
68. The beverage of claim 62 wherein said magnesium comprises from about 1 to about 3 mEq/L.
69. A beverage composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
70. The beverage of claim 69 wherein said sodium comprises up to about 100 mEq/L.
71. The beverage of claim 69 wherein said sodium comprises up to about 60 mEq/L.
72. The beverage of claim 69 wherein said sodium comprises from about 33 to about 40 mEq/L.
73. The beverage of claim 69 wherein said chloride comprises from about 10 to about 50 mEq/L.
74. The beverage of claim 69 wherein said chloride comprises from about 11 to about 20 mEq/L.
75. The beverage of claim 69 wherein said potassium comprises from about 8 to about 16 mEq/L.
76. The beverage of claim 69 further comprising calcium from about 0.1 to about 6 mEq/L.
77. The beverage of claim 69 further comprising magnesium from about 0.1 to about 6 mEq/L.
78. A beverage composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium;
(e) at least about 0.1 to about 6 mEq/L of magnesium;
(f) at least about 0.1 to about 6 mEq/L of calcium;
(g) from about 0 to about 0.4% by weight of a flavoring;
(h) from about 0 to about 100 ppm of a clouding agent;
(i) from about 0.24 to about 0.45% by weight of citric acid; and
(j) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
79. The beverage of claim 78 wherein said sodium comprises up to about 100 mEq/L.
80. The beverage of claim 78 wherein said sodium comprises up to about 60 mEq/L.
81. The beverage of claim 78 wherein said sodium comprises from about 33 to about 40 mEq/L.
82. The beverage of claim 78 wherein said chloride comprises from about 10 to about 50 mEq/L.
83. The beverage of claim 78 wherein said chloride comprises from about 11 to about 20 mEq/L.
84. The beverage of claim 78 wherein said potassium comprises from about 8 to about 16 mEq/L.
85. A beverage composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 1.0 mEq/L of magnesium;
(e) at least about 0.1 mEq/L of calcium; and
(f) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
86. The beverage of claim 85 wherein said sodium comprises from about 30 to about 100 mEq/L.
87. The beverage of claim 85 wherein said chloride comprises from about 10 to about 50 mEq/L.
88. The beverage of claim 85 wherein said magnesium comprises from about 1 to about 6 mEq/L.
89. The beverage of claim 85 wherein said calcium comprises from about 0.1 to about 6 mEq/L.
90. The beverage of claim 85 further comprising at least 7 mEq/L of potassium.
91. The beverage of claim 90 wherein said potassium comprises from about 7 to about 25 mEq/L.
92. The beverage of claim 91 further comprising:
(a) from about 0 to about 0.4% by weight of a flavoring;
(b) from about 0 to about 100 ppm of a clouding agent; and
(c) from about 0.24 to about 0.45% by weight citric acid.
93. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride; and
(d) at least about 8 mEq/L of potassium;
wherein the osmolality of said fluid composition is in the range of from about 250 to about 350 mOsm/Kg.
94. The concentrate composition of claim 93 wherein said liquid is water.
95. The concentrate composition of claim 93 wherein said liquid is a sports beverage.
96. The concentrate composition of claim 93 wherein said liquid includes at least one electrolyte.
97. The concentrate composition of claim 93 wherein said liquid includes a carbohydrate.
98. The concentrate composition of claim 93 wherein said liquid includes at least one electrolyte and a carbohydrate.
99. The concentrate composition of claim 93 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
100. The concentrate composition of claim 93 wherein said carbohydrate source is a mixture of a minimum of three of the following types of carbohydrates: sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate with the amount of fructose, if selected, always less than the total amount of glucose from all sources.
101. The concentrate composition of claim 93 wherein said sodium comprises from about 30 to about 100 mEq/L.
102. The concentrate composition of claim 93 wherein said sodium comprises from about 30 to about 60 mEq/L.
103. The concentrate composition of claim 93 wherein said sodium comprises from about 33 to about 40 mEq/L.
104. The concentrate composition of claim 101 wherein said sodium comprises from about 10 to about 50 mEq/L of sodium chloride and from about 10 to about 50 mEq/L of sodium citrate.
105. The concentrate composition of claim 102 wherein said sodium comprises from about 10 to about 30 mEq/L of sodium chloride and from about 10 to about 30 mEq/L of sodium citrate.
106. The concentrate composition of claim 93 wherein said chloride comprises from about 11 to about 18 mEq/L.
107. The concentrate composition of claim 93 wherein said potassium comprises from about 8 to about 20 mEq/L.
108. The concentrate composition of claim 93 that, when constituted with a liquid, produces a fluid composition further comprising calcium from about 1 to about 6 mEq/L.
109. The concentrate composition of claim 93 that, when constituted with a liquid, produces a fluid composition further comprising magnesium from about 1 to about 6 mEq/L.
110. The concentrate composition of claim 93 that, when constituted with a liquid, produces a fluid composition further comprising a flavoring from about 0 to about 0.4% by weight.
111. The concentrate composition of claim 93 that, when constituted with a liquid, produces a fluid composition further comprising a clouding agent from about 0 to about 100 parts per million.
112. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 8 mEq/L of potassium;
(e) from about 1 to about 6 mEq/L of calcium;
(f) from about 1 to about 6 mEq/L of magnesium;
(g) from about 0 to about 0.4% by weight of a flavoring agent;
(h) from about 0 to about 100 parts per million of a clouding agent; and
(i) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
113. The concentrate composition of claim 112 wherein said sodium comprises from about 30 to about 100 mEq/L.
114. The concentrate composition of claim 112 wherein said sodium comprises from about 30 to about 60 mEq/L.
115. The concentrate composition of claim 112 wherein said sodium comprises from about 33 to about 40 mEq/L.
116. The concentrate composition of claim 113 wherein said sodium comprises from about 10 to about 50 mEq/L of sodium chloride and from about 10 to about 50 mEq/L of sodium citrate.
117. The concentrate composition of claim 114 wherein said sodium comprises from about 10 to about 30 mEq/L of sodium chloride and from about 10 to about 30 mEq/L of sodium citrate.
118. The concentrate composition of claim 112 wherein said chloride comprises from about 11 to about 18 mEq/L.
119. The concentrate composition of claim 112 wherein said potassium comprises from about 8 to about 20 mEq/L.
120. The concentrate composition of claim 112 wherein said calcium comprises from about 1 to about 3 mEq/L.
121. The concentrate composition of claim 112 wherein said magnesium comprises from about 1 to about 3 mEq/L.
122. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4.5 to about 6.5% by weight of carbohydrates;
(b) from about 30 to about 40 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) from at about 8 to about 20 mEq/L of potassium;
(e) from about 1 to about 3 mEq/L of calcium;
(f) from about 1 to about 3 mEq/L of magnesium;
(g) from about 0 to about 0.2% by weight of a flavoring agent;
(h) from about 0 to about 50 parts per million of a clouding agent; and
(i) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
123. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment; and;
(c) at least about 8 mEq/L of potassium; and
(d) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
124. The concentrate composition of claim 123 wherein said carbohydrate source is selected from sucrose, maltose, maltodextrin, glucose, galactose, trehalose, fructose, fructo-oligosaccharides, beta-glucan, and trioses such as pyruvate and lactate and mixtures thereof.
125. The concentrate composition of claim 123 that, when constituted with a liquid, produces a fluid composition further comprising from about 1 to about 6 mEq/L of calcium.
126. The concentrate composition of claim 123 that, when constituted with a liquid, produces a fluid composition further comprising from about 1 to about 6 mEq/L of magnesium.
127. The concentrate composition of claim 123 that, when constituted with a liquid, produces a fluid composition further comprising:
(a) from about 0 to about 0.4% by weight of a flavoring agent; and
(b) from about 0 to about 100 parts per million of a clouding agent.
128. The concentrate composition of claim 127 that, when constituted with a liquid, produces a fluid composition further comprising from about 1 to about 6 mEq/L of calcium.
129. The concentrate composition of claim 128 that, when constituted with a liquid, produces a fluid composition further comprising from about 1 to about 6 mEq/L of magnesium.
130. The concentrate composition of claim 123 wherein said ions which favor the filling of the extracellular fluid compartment comprise sodium and chloride ions.
131. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment;
(c) at least about 8 mEq/L of potassium;
(d) from about 1 to about 6 mEq/L of calcium;
(e) from about 1 to about 6 mEq/L of magnesium;
(f) from about 0 to about 0.4% by weight of a flavoring agent;
(g) from about 0 to about 100 parts per million of a clouding agent; and
(h) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
132. The concentrate composition of claim 131 wherein said ions which favor the filling of the extracellular fluid compartment comprise sodium and chloride ions.
133. The concentrate composition of claim 131 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 42 to about 70 mEq/L.
134. The concentrate composition of claim 131 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 46 to about 60 mEq/L.
135. The concentrate composition of claim 131 wherein said potassium comprises from about 8 to about 20 mEq/L.
136. The concentrate composition of claim 131 wherein said calcium comprises from about 1 to about 3 mEq/L.
137. The concentrate composition of claim 131 wherein said magnesium comprises from about 1 to about 3 mEq/L.
138. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment;
(c) from about 8 to about 20 mEq/L of potassium;
(d) from about 1 to about 6 mEq/L of calcium;
(e) from about 1 to about 6 mEq/L of magnesium;
(f) from about 0 to about 0.4% by weight of a flavoring agent;
(g) from about 0 to about 100 parts per million of a clouding agent; and
(h) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
139. The concentrate composition of claim 138 wherein said ions which favor the filling of the extracellular fluid compartment comprise sodium and chloride ions.
140. The concentrate composition of claim 138 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 42 to about 70 mEq/L.
141. The concentrate composition of claim 138 wherein said ions which favor the filling of the extracellular fluid compartment comprises from about 46 to about 60 mEq/L.
142. The concentrate composition of claim 138 wherein said potassium comprises from about 10 to about 19 mEq/L.
143. The concentrate composition of claim 138 wherein said calcium comprises from about 1 to about 3 mEq/L.
144. The concentrate composition of claim 138 wherein said magnesium comprises from about 1 to about 3 mEq/L.
145. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
146. The concentrate composition of claim 145 wherein said sodium comprises up to about 100 mEq/L.
147. The concentrate composition of claim 145 wherein said sodium comprises up to about 60 mEq/L.
148. The concentrate composition of claim 145 wherein said sodium comprises from about 33 to about 40 mEq/L.
149. The concentrate composition of claim 145 wherein said chloride comprises from about 10 to about 50 mEq/L.
150. The concentrate composition of claim 145 wherein said chloride comprises from about 11 to about 20 mEq/L.
151. The concentrate composition of claim 145 wherein said potassium comprises from about 8 to about 16 mEq/L.
152. The concentrate composition of 145 that, when constituted with a liquid, produces a fluid composition further comprising calcium from about 0.1 to about 6 mEq/L.
153. The concentrate composition of 145 that, when constituted with a liquid, produces a fluid composition further comprising magnesium from about 0.1 to about 6 mEq/L.
154. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium;
(e) at least about 0.1 to about 6 mEq/L of magnesium;
(f) at least about 0.1 to about 6 mEq/L of calcium;
(g) from about 0 to about 0.4% by weight of a flavoring;
(h) from about 0 to about 100 ppm of a clouding agent;
(i) from about 0.24 to about 0.45% by weight of citric acid; and
(j) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
155. The concentrate composition of claim 154 wherein said sodium comprises up to about 100 mEq/L.
156. The concentrate composition of claim 154 wherein said sodium comprises up to about 60 mEq/L.
157. The concentrate composition of claim 154 wherein said sodium comprises from about 33 to about 40 mEq/L.
158. The concentrate composition of claim 154 wherein said chloride comprises from about 10 to about 50 mEq/L.
159. The concentrate composition of claim 154 wherein said chloride comprises from about 11 to about 20 mEq/L.
160. The concentrate composition of claim 154 wherein said potassium comprises from about 8 to about 16 mEq/L.
161. A concentrate composition that, when constituted with a liquid, produces a fluid composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 1.0 mEq/L of magnesium;
(e) at least about 0.1 mEq/L of calcium; and
(f) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
162. The concentrate composition of claim 161 wherein said sodium comprises from about 30 to about 100 mEq/L.
163. The concentrate composition of claim 161 wherein said chloride comprises from about 10 to about 50 mEq/L.
164. The concentrate composition of claim 161 wherein said magnesium comprises from about 1 to about 6 mEq/L.
165. The concentrate composition of claim 161 wherein said calcium comprises from about 0.1 to about 6 mEq/L.
166. The concentrate composition of claim 161 that, when constituted with a liquid, produces a fluid composition further comprising at least 7 mEq/L of potassium.
167. The concentrate composition of claim 166 wherein said potassium comprises from about 7 to about 25 mEq/L.
168. The concentrate composition of claim 167 that, when constituted with a liquid, produces a fluid composition further comprising:
(a) from about 0 to about 0.4% by weight of a flavoring;
(b) from about 0 to about 100 ppm of a clouding agent; and
(c) from about 0.24 to about 0.45% by weight citric acid.
169. A method of reducing the effects of dehydration, said method comprising administering before, during or after activity-induced fluid loss a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 7 mEq/L of potassium; and
(e) water;
wherein the osmolality of said fluid composition is in the range of from about 250 to about 350 mOsm/Kg.
170. A method of reducing the effects of dehydration, said method comprising administering before, during or after activity-induced fluid loss a fluid composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment; and;
(c) at least about 8 mEq/L of potassium; and
(d) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
171. A method of reducing the effects of dehydration, said method comprising administering before, during or after activity-induced fluid loss a fluid composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
172. A method of reducing the effects of dehydration, said method comprising administering before, during or after activity-induced fluid loss a fluid composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 1.0 mEq/L of magnesium;
(e) at least about 0.1 mEq/L of calcium; and
(f) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
173. A method of improving fluid retention, said method comprising administering a composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 7 mEq/L of potassium; and
(e) water;
wherein the osmolality of said composition is in the range of from about 250 to about 350 mOsm/Kg.
174. A method of improving fluid retention, said method comprising administering a composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment; and;
(c) at least about 8 mEq/L of potassium; and
(d) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
175. A method of improving fluid retention, said method comprising administering a composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
176. A method of improving fluid retention, said method comprising administering a composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 1.0 mEq/L of magnesium;
(e) at least about 0.1 mEq/L of calcium; and
(f) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
177. A method of abating urinary loss, said method comprising administering a composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride;
(d) at least about 7 mEq/L of potassium; and
(e) water;
wherein the osmolality of said composition is in the range of from about 250 to about 350 mOsm/Kg.
178. A method of abating urinary loss, said method comprising administering a composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment; and;
(c) at least about 8 mEq/L of potassium; and
(d) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
179. A method of abating urinary loss, said method comprising administering a composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
180. A method of abating urinary loss, said method comprising administering a composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 1.0 mEq/L of magnesium;
(e) at least about 0.1 mEq/L of calcium; and
(f) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
181. A method of enhancing rehydration, improving fluid retention, and reducing urinary fluid loss, said method comprising administering orally or intravenously a composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) from about 10 to about 20 mEq/L of chloride; and
(d) at least about 7 mEq/L of potassium.
182. A method of enhancing rehydration, improving fluid retention, and reducing urinary fluid loss, said method comprising administering orally or intravenously a composition comprising:
(a) from about 4% to about 10% by weight of a carbohydrate source;
(b) from about 40 to about 78 mEq/L of ions which favor the filling of the extracellular fluid compartment; and;
(c) at least about 8 mEq/L of potassium; and
(d) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
183. A method of enhancing rehydration, improving fluid retention, and reducing urinary fluid loss, said method comprising administering orally or intravenously a composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) greater than about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 3 to about less than 16 mEq/L of potassium; and
(e) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
184. A method of enhancing rehydration, improving fluid retention, and reducing urinary fluid loss, said method comprising administering orally or intravenously a composition comprising:
(a) from about 4 to about 10% by weight of a carbohydrate source;
(b) at least about 30 mEq/L of sodium;
(c) at least about 10 mEq/L of chloride;
(d) at least about 1.0 mEq/L of magnesium;
(e) at least about 0.1 mEq/L of calcium; and
(f) water;
wherein the osmolality of said beverage is in the range of from about 250 to about 350 mOsm/Kg.
Priority Applications (16)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/706,420 US20050100637A1 (en) | 2003-11-12 | 2003-11-12 | Carbohydrate and electrolyte replacement composition |
PE2004001102A PE20050650A1 (en) | 2003-11-12 | 2004-11-11 | CARBOHYDRATE AND ELECTROLYTE REPLACEMENT COMPOSITION |
GT200400233A GT200400233A (en) | 2003-11-12 | 2004-11-11 | REPLACEMENT COMPOSITION OF CARBOHYDRATES AND ELECTROLYTES |
AU2004289359A AU2004289359B2 (en) | 2003-11-12 | 2004-11-12 | Carbohydrate and electrolyte replacement composition |
RU2006120415/13A RU2006120415A (en) | 2003-11-12 | 2004-11-12 | COMPOSITION FOR COMPLETING THE LOSS OF CARBOHYDRATES AND ELECTROLYTES |
KR1020067011548A KR100878133B1 (en) | 2003-11-12 | 2004-11-12 | Carbohydrate and electrolyte replacement composition |
PCT/US2004/038155 WO2005046360A2 (en) | 2003-11-12 | 2004-11-12 | Carbohydate and electrolyte replacement composition |
IT000797A ITTO20040797A1 (en) | 2003-11-12 | 2004-11-12 | COMPOSITION FOR THE REPLACEMENT OF CARBOHYDRATES AND ELECTROLYTES. |
ARP040104190A AR046706A1 (en) | 2003-11-12 | 2004-11-12 | COMPOSITION FOR THE REPLACEMENT OF CARBOHYDRATES AND ELECTROLYTES |
CA002545606A CA2545606A1 (en) | 2003-11-12 | 2004-11-12 | Carbohydrate and electrolyte replacement composition |
JP2006539978A JP2007510758A (en) | 2003-11-12 | 2004-11-12 | Composition for supplementing carbohydrates and electrolytes |
BRPI0416505-5A BRPI0416505A (en) | 2003-11-12 | 2004-11-12 | carbohydrate and electrolyte replacement composition |
EP04811040A EP1689254A2 (en) | 2003-11-12 | 2004-11-12 | Carbohydate and electrolyte replacement composition |
AU2008212089A AU2008212089B2 (en) | 2003-11-12 | 2008-09-09 | Carbohydrate and electrolyte replacement composition |
US12/273,609 US7993690B2 (en) | 2003-11-12 | 2008-11-19 | Carbohydrate and electrolyte replacement composition |
US13/177,675 US20110262596A1 (en) | 2003-11-12 | 2011-07-07 | Carbohydrate And Electrolyte Replacement Composition |
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KR (1) | KR100878133B1 (en) |
AR (1) | AR046706A1 (en) |
AU (2) | AU2004289359B2 (en) |
BR (1) | BRPI0416505A (en) |
CA (1) | CA2545606A1 (en) |
GT (1) | GT200400233A (en) |
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PE (1) | PE20050650A1 (en) |
RU (1) | RU2006120415A (en) |
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Also Published As
Publication number | Publication date |
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AU2008212089A1 (en) | 2008-10-02 |
PE20050650A1 (en) | 2005-08-25 |
WO2005046360A3 (en) | 2005-07-14 |
AR046706A1 (en) | 2005-12-21 |
AU2004289359A1 (en) | 2005-05-26 |
AU2004289359B2 (en) | 2008-06-12 |
AU2008212089B2 (en) | 2009-11-19 |
GT200400233A (en) | 2005-08-29 |
JP2007510758A (en) | 2007-04-26 |
WO2005046360A2 (en) | 2005-05-26 |
KR100878133B1 (en) | 2009-01-14 |
CA2545606A1 (en) | 2005-05-26 |
US20110262596A1 (en) | 2011-10-27 |
US7993690B2 (en) | 2011-08-09 |
EP1689254A2 (en) | 2006-08-16 |
BRPI0416505A (en) | 2007-01-09 |
RU2006120415A (en) | 2007-12-27 |
US20090148566A1 (en) | 2009-06-11 |
KR20060130583A (en) | 2006-12-19 |
ITTO20040797A1 (en) | 2005-02-12 |
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