US20040206093A1

US20040206093A1 - Illumination device in a cryostat

Info

Publication number: US20040206093A1
Application number: US10/824,211
Authority: US
Inventors: Claudia Dorenkamp; Stefan Kuenkel; Gerhard Oppl; Peter Scheck
Original assignee: Leica Microsystems Nussloch GmbH
Current assignee: Leica Biosystems Nussloch GmbH
Priority date: 2003-04-19
Filing date: 2004-04-14
Publication date: 2004-10-21
Also published as: CN1538160A; DE10318026C5; GB0403255D0; JP2004317515A; GB2400645A; DE20321622U1; CN100498271C; DE10318026B4; GB2400645B; DE10318026A1

Abstract

A cryostat (1), having a cryostat chamber (2) in which a microtome for cutting frozen specimens is arranged, is described. An illumination device (4) for illuminating the cryostat chamber (2) is provided, the illumination device (4) comprising at least one LED (5).

Description

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims priority of the German patent application 103 18 026.5 filed Apr. 19, 2003 which is incorporated by reference herein.

FIELD OF THE INVENTION

The invention concerns a cryostat having a cryostat chamber in which a microtome for cutting frozen specimens is arranged, and in particular an illumination device for the cryostat chamber.

BACKGROUND OF THE INVENTION

Cryostats of this kind are used in order to cut frozen specimens with a microtome for subsequent viewing with a microscope. The specimens to be cut are cooled to a specific predefined temperature, the temperatures generally being between −10° C. and −50° C. To ensure temperature invariability, the microtomes are arranged in extensively encapsulated cryostat chambers, and the latter are appropriately cooled.

To ensure safe operation, the cryostat chambers are illuminated with lamps. This has the disadvantage, however, that these light sources generate heat, thus jeopardizing the temperature invariability that is critical for cutting the specimen.

It has moreover been found that the light sources, whose dimensions are quite large, cannot be effectively cleaned.

SUMMARY OF THE INVENTION

It is therefore the object of the present invention to improve the illumination of cryostat chambers so as to result in greater temperature invariability inside the chamber.

This object is achieved, according to the present invention, by way of an illumination device including at least one LED for illuminating the cryostat chamber.

The invention is characterized in that the illumination device comprises as the light source at least one LED, preferably a white-light LED. A compact configuration, very low thermal emission, and good cleaning and disinfection capability are achieved with the LED. In addition, the LEDs can also be very effectively protected against water splashes because of their physical shape.

In an embodiment of the invention, multiple LEDs are grouped together into an illumination module, resulting in an increased luminance with a compact configuration. Furthermore, it is thus also very easy to protect the electrical contacts of the LEDs from moisture or water splashes.

In a development of the invention, the illumination module comprises a housing having a plug connector for supplying power. The result of this is that the illumination module can be arranged replaceably, by way of the plug connector, in the cryostat chamber. Single or multiple illumination modules can thus be replaceably arranged, as needed, in prepared plug-in receptacles in the cryostat chamber.

In a further embodiment of the invention, multiple illumination modules are electrically connected to a common power supply system. This power supply system is preferably arranged outside the cryostat chamber, so that not only minimized complexity but also reduced heat evolution is implemented here.

In a development of the invention, a circuit arrangement is provided for switching single and/or multiple illumination modules on and off. Single regions inside the cryostat chamber can thus be illuminated depending on the application.

In an advantageous development, the circuit arrangement additionally has the function of a dimmer for single and/or multiple illumination modules. This likewise makes possible individual illumination of the cryostat chamber

In a development of the invention, a switch is arranged on the cryostat and is electrically connected to the circuit arrangement, the switch automatically switching and/or dimming single and/or multiple illumination modules upon opening and/or closing of the cryostat chamber. The result of this function is that the illuminating light inside the chamber is automatically controlled when the cover or panel is opened by an operator. It is thus possible, for example, always to switch to optimum illumination upon opening, and to switch off or dim single illumination modules upon closing. It seems advisable, for example, to illuminate only the sample holder and/or the cutting knife of the microtome when the cryostat chamber is closed.

In a further embodiment of the invention, a water-splash protector is associated with at least one LED. Advantageously, the illumination module can be equipped with a seal for encapsulating the electrical LED contacts.

BRIEF DESCRIPTION OF THE DRAWINGS

The invention will be explained in more detail on the basis of an exemplary embodiment, referring to the schematic drawings in which: [0016]
FIG. 1 is a view of a cryostat formed in accordance with an embodiment of the present invention; [0017]
FIG. 2 is a section through an illumination module of the cryostat; [0018]
FIG. 3 is a plan view of the illumination module; and [0019]
FIG. 4 is a plan view of the cryostat with a cryostat chamber.[0020]

DETAILED DESCRIPTION OF THE INVENTION

FIG. 1 is a view of [0021] cryostat 1 having a cryostat chamber 2 in which an illumination device 4, having multiple illumination modules 6, is arranged. Cryostat chamber 2 can be opened and closed by way of a movable cover 17. Illumination modules 6 are electrically connected via leads 14 to a circuit arrangement 10, and via lead 15 to a power supply system 9. A switch 11, which detects the opening and closing of cover 17 of cryostat chamber 2, is arranged on cryostat 1. Switch 11 is likewise connected via a lead (not depicted) to the circuit arrangement 10.
[0022] Single illumination modules 6, or even all of them, can be switched and/or dimmed via circuit arrangement 10. By means of switch 11, single and/or multiple illumination modules 6 can be automatically switched and/or dimmed when cover 17 or cryostat chamber 2 is opened or closed.
FIG. 2 is a section through an [0023] illumination module 6 having a housing 7 and having single LEDs 5 that are arranged on a circuit board 13. Circuit board 13 and the electrical contacts of LEDs 5 are sealed with respect to external influence by way of a water-splash protector 12 provided in the housing.
FIG. 3 shows [0024] illumination module 6 in a plan view, with a plug connector 8 arranged on the side of housing 7. By way of plug connector 8, illumination module 6 can be mounted in cryostat chamber 2 and simultaneously supplied with power.
FIG. 4 is a plan view of [0025] cryostat chamber 2 with the centrally arranged microtome 3 and illumination modules 6 provided around microtome 3. Uniform illumination of all regions inside cryostat 1 is achieved with this arrangement. Also provided in cryostat chamber 2, in addition to microtome 3, is a freezing bar 16 for rapid freezing of specimens and for storing multiple specimen holders. A separate illumination module 6 is arranged for illuminating freezing bar 16.
PARTS LIST [0026]
Cryostat [0027]
[0028] 2 Cryostat chamber
[0029] 3 Microtome
[0030] 4 Illumination device
[0031] 5 LED
[0032] 6 Illumination module
[0033] 7 Housing
[0034] 8 Plug connector
[0035] 9 Power supply system
[0036] 10 Circuit arrangement
[0037] 11 Switch
[0038] 12 Water-splash protector
[0039] 13 Circuit board
[0040] 14 Leads
[0041] 15 Power lead
[0042] 16 Freezing bar
[0043] 17 Cover

Claims

What is claimed is:

1. A cryostat comprising:

a cryostat chamber;

a microtome inside the cryostat chamber for cutting frozen specimens; and

an illumination device including at least one LED for illuminating the cryostat chamber.

2. The cryostat as defined in claim 1, wherein the illumination device includes an illumination module having a plurality of LEDs.

3. The cryostat as defined in claim 2, wherein the illumination module comprises a housing having a plug connector for connecting a power supply system to the illumination module.

4. The cryostat as defined in claim 2, further comprising a power supply system, wherein a plurality of the illumination modules are provided and electrically connected to the power supply system.

5. The cryostat as defined in claim 2, further comprising a circuit arrangement for controlling the illumination module.

6. The cryostat as defined in claim 4, further comprising a circuit arrangement for individually and/or collectively controlling the plurality of illumination modules.

7. The cryostat (1) as defined in claim 5, wherein the circuit arrangement enables dimming of the illumination module.

8. The cryostat as defined in claim 6, wherein the circuit arrangement enables individual and/or collective dimming of the plurality of illumination modules.

9. The cryostat as defined in claim 4, wherein the cryostat chamber includes a cover that can be opened and closed, and the cryostat further comprises a switch electrically connected to the circuit arrangement and activated by opening and closing of the cover, whereby the switch automatically signals the circuit arrangement to control at least one of the plurality of illumination modules upon opening and closing of the cover.

10. The cryostat as defined in claim 2, wherein the illumination module includes a splash protector for protecting the plurality of LEDs.