Search Images Maps Play YouTube News Gmail Drive More »
Sign in
Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader.

Patents

  1. Advanced Patent Search
Publication numberUS20040068165 A1
Publication typeApplication
Application numberUS 10/678,342
Publication date8 Apr 2004
Filing date3 Oct 2003
Priority date31 Mar 2000
Also published asCA2404618A1, CA2404618C, CN1191475C, CN1432132A, DE60122517D1, DE60122517T2, EP1292825A2, EP1292825B1, US7050843, US7548772, US20050059872, US20060200021, WO2001075438A2, WO2001075438A3
Publication number10678342, 678342, US 2004/0068165 A1, US 2004/068165 A1, US 20040068165 A1, US 20040068165A1, US 2004068165 A1, US 2004068165A1, US-A1-20040068165, US-A1-2004068165, US2004/0068165A1, US2004/068165A1, US20040068165 A1, US20040068165A1, US2004068165 A1, US2004068165A1
InventorsRobert Shartle, Timothy Ohara, Mahyar Kermani
Original AssigneeShartle Robert Justice, Ohara Timothy J., Kermani Mahyar Z.
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Electrically-conductive patterns for monitoring the filling of medical devices
US 20040068165 A1
Abstract
A flexible diagnostic device has a measurement cell that is sandwiched between the conductive surfaces of two conductive-coated insulating layers. At least one of the conductive surfaces is scored with an insulating pattern, so that the flow of a conductive fluid sample into the cell can be monitored.
Images(8)
Previous page
Next page
Claims(16)
We claim:
1. A medical diagnostic device for measuring an analyte concentration of an electrically conductive biological fluid, comprising a multilayer structure having a first layer and a second layer sandwiching an intermediate layer,
a) the first and second layers each comprising an insulating sheet, having a conductive surface adjoining the intermediate layer,
b) the intermediate layer being an insulating layer with a cutout, having a first end and a second end, which, together with the first and second layers, defines a flow channel to permit the sample to flow from the first end to the second end,
c) the flow channel comprising
(i) a dry reagent on the conductive surface of one of the layers for reacting with the sample to yield a change in an electrical parameter that can be related to the analyte concentration of the fluid and
(ii) an electrochemical cell, within which the electrical parameter is measured,
d) the conductive surface of one of the layers having a first insulating pattern scored into its conductive surface to divide the second layer into two regions, insulated from each other, whereby sample that flows across the pattern provides a conductive path from the first end to the second end.
2. The device of claim 1, in which the first end of the cutout is at a first edge of the intermediate layer and the second end is at a second edge of the intermediate layer, opposite the first edge.
3. The device of claim 1, in which the dry reagent is on the conductive surface of the first layer and the insulating pattern is scored into the conductive surface of the second layer.
4. The device of claim 1, in which sample that enters the flow channel at the first end flows through the electrochemical cell, before it reaches the first insulating pattern.
5. The device of claim 1, in which the biological fluid is blood and the analyte being measured is glucose.
6. The device of claim 1, in which the first and second layers each comprise metallized thermoplastic sheets.
7. The device of claim 1, in which the intermediate layer comprises a thermoplastic sheet having adhesive on both surfaces for adhering to the first and second layers.
8. The device of claim 1, in which the reagent on the conductive surface comprises a buffer, a mediator, and an enzyme.
9. The device of claim 1, in which the flow channel is a capillary channel and the insulating pattern scored into the conductive surface has at least one serration within the flow channel.
10. The device of claim 9, in which the insulating pattern has at least one serration within the flow channel pointing toward each end of the channel.
11. The device of claim 1, further comprising a second insulating pattern scored into the conductive surface of the scored layer between the first end and the first insulating pattern to divide the scored layer into three regions, insulated from each other.
12. The device of claim 11, in which sample that enters the flow channel at the first end reaches the second insulating pattern before it flows through the electrochemical cell.
13. The device of claim 1, further comprising electrical circuit means for detecting the flow of fluid through the flow channel.
14. A method for preparing an electrically-conductive pattern comprising passing a web of a conductive-coated flexible insulator between a cutting die and anvil, in which the cutting die has a cutting element that is raised a height greater than the thickness of the conductive coating for scoring through preselected portions of the conductive coating.
15. The method of claim 14, in which the cutting die and anvil are rollers.
16. The method of claim 14, in which the conductive coating has a thickness in the range from about 5 to about 100 nm and the cutting element is raised about one thousand times the coating thickness.
Description
    BACKGROUND OF THE INVENTION
  • [0001]
    1. Field of the Invention
  • [0002]
    This invention relates to a diagnostic device that has an insulating pattern scored into a conductive coating on the device to facilitate analytical measurements; more particularly, to monitor filling of the device.
  • [0003]
    2. Description of the Related Art
  • [0004]
    A variety of medical diagnostic procedures involve tests on biological fluids, such as blood, urine, or saliva, to determine an analyte concentration in the fluid. The procedures measure a variety of physical parameters—mechanical, optical, electrical, etc.,—of the biological fluid.
  • [0005]
    Among the analytes of greatest interest is glucose, and dry phase reagent strips incorporating enzyme-based compositions are used extensively in clinical laboratories, physicians' offices, hospitals, and homes to test samples of biological fluids for glucose concentration. In fact, reagent strips have become an everyday necessity for many of the nation's estimated 16 million people with diabetes. Since diabetes can cause dangerous anomalies in blood chemistry, it can contribute to vision loss, kidney failure, and other serious medical consequences. To minimize the risk of these consequences, most people with diabetes must test themselves periodically, then adjust their glucose concentration accordingly, for instance, through diet, exercise, and/or insulin injections. Some patients must test their blood glucose concentration as often as four times or more daily.
  • [0006]
    One type of glucose measurement system operates electrochemically, detecting the oxidation of blood glucose on a dry reagent strip. The reagent generally includes an enzyme, such as glucose oxidase or glucose dehydrogenase, and a redox mediator, such as ferrocene or ferricyanide. This type of measurement system is described in U.S. Pat. No. 4,224,125, issued on Sep. 23, 1980, to Nakamura et al.; and U.S. Pat. No. 4,545,382, issued on Oct. 8, 1985, to Higgins et al., incorporated herein by reference.
  • [0007]
    Hodges et al., WO 9718464 A1, published on May 22, 1997, discloses an electrochemical device for measuring blood glucose that includes two metallized polyethylene terephthalate (PET) layers sandwiching an adhesive-coated PET intermediate layer. The metallized layers constitute first and second electrodes, and a cutout in the adhesive-coated layer defines an electrochemical cell. The cell contains the reagent that reacts with the glucose in a blood sample. The device is elongated, and the sample is introduced at an inlet on one of the long sides.
  • [0008]
    Nakai et al., U.S. Pat. No. 5,266,179, issued on Nov. 30, 1993, discloses an electrochemical system for measuring blood glucose, in which the sample application time is determined by a resistance drop between a pair of electrodes to which a constant voltage was applied.
  • [0009]
    White et al., U.S. Pat. No. 5,366,609, issued on Nov. 22, 1994, describes the same principle of monitoring the resistance drop between the electrodes to determine the time at which blood was applied to a dry glucose reagent strip. In both patents, a constant voltage is applied between working and reference electrodes to track resistance changes that result from the introduction of a blood sample to a dry reagent strip.
  • [0010]
    Accurately determining an analyte concentration generally requires a sufficient supply of sample. Yoshioka et al., U.S. Pat. No. 5,264,103, issued on Nov. 23, 1993, discloses a biosensor for electrochemically measuring concentration of an analyte, such as glucose, in a biological fluid. An impedance change indicates that a sufficient supply of sample has been supplied to the sensor.
  • [0011]
    Littlejohn et al., U.S. Pat. No. 4,940,945, issued on Jul. 10, 1990, discloses a portable apparatus that can measure pH of a blood sample. The apparatus detects the presence of a sample in a cell by injecting a constant current between a fill electrode outside the sample chamber and one of two electrodes inside the chamber. When the impedance decreases by at least two orders of magnitude, the meter recognizes that sufficient sample has been provided and emits a beep. The fill electrode is then cut out of the circuit that includes the two electrodes inside the sample cell, and measurements are made potentiometrically.
  • [0012]
    Crismore et al., U.S. Pat. No. 5,997,817, issued on Dec. 7, 1999, discloses an electrochemical sensor strip that includes a window through which a user can determine visually whether enough sample has been applied to the strip.
  • [0013]
    None of the above references discloses a mechanism for monitoring the movement of a blood sample into (and through) an electrochemical cell.
  • SUMMARY OF THE INVENTION
  • [0014]
    This invention provides a medical diagnostic device for measuring an analyte concentration of an electrically conductive biological fluid. The device comprises a multilayer structure having a first layer and a second layer sandwiching an intermediate layer,
  • [0015]
    a) the first and second layers each comprising an insulating sheet, having a conductive surface adjoining the intermediate layer,
  • [0016]
    b) the intermediate layer being an insulating layer with a cutout, having a first end and a second end, which, together with the first and second layers, defines a flow channel to permit the sample to flow from the first end to the second end,
  • [0017]
    c) the flow channel comprising (i) a dry reagent on the conductive surface of one of the layers, for reacting with the sample to yield a change in an electrical parameter that can be related to the analyte concentration of the fluid, and
  • [0018]
    (ii) an electrochemical cell, within which the electrical parameter is measured,
  • [0019]
    d) the conductive surface of one of the layers having a first insulating pattern scored into its conductive surface to divide the layer into two regions, insulated from each other, whereby sample that flows across the pattern provides a conductive path from the first end to the second end.
  • [0020]
    For convenience, we refer to “one of” the layers in the above description and the claims, but we do not intend that phrase to exclude “both” layers.
  • [0021]
    A method for preparing an electrically conductive pattern of the present invention comprises passing a web of a conductive-coated flexible insulator between a cutting die and anvil, in which the cutting die has a cutting element that is raised a height greater than the thickness of the conductive coating for scoring through preselected portions of the conductive coating.
  • [0022]
    The present invention provides a medical diagnostic device that can easily sense when an adequate sample of a conductive biological fluid has been introduced into the device, without relying on the vision of the user. When the device measures glucose concentration, the user generally has diabetes and is frequently vision-impaired. In another embodiment, the invention provides a method for preparing an element of the diagnostic device. The method is well adapted for a high-speed, continuous line production process.
  • BRIEF DESCRIPTION OF THE DRAWINGS.
  • [0023]
    [0023]FIG. 1 is an exploded perspective view of a device of the present invention.
  • [0024]
    [0024]FIG. 2 is a plan view of another device of the present invention.
  • [0025]
    [0025]FIG. 3A schematically depicts the operation of a stop junction in stopping fluid flow through a capillary channel.
  • [0026]
    FIGS. 3B, and 3C schematically depict fluid flow through a capillary channel of the device of FIG. 2.
  • [0027]
    [0027]FIG. 4 is a cross-section through the device of FIG. 2.
  • [0028]
    [0028]FIG. 5 is a block diagram of a fill-detection circuit of the present invention.
  • [0029]
    [0029]FIG. 6 is an exploded perspective view of an alternative embodiment of the device of FIG. 1.
  • [0030]
    [0030]FIG. 7 depicts an apparatus for practicing a method of the present invention.
  • DETAILED DESCRIPTION OF THE INVENTION
  • [0031]
    This invention relates to an electrochemical method of measuring an analyte concentration of an electrically conductive biological fluid. In the interest of brevity, the description below emphasizes amperometrically measuring glucose concentration in samples of whole blood; however, the person of ordinary skill in the medical diagnostics art will recognize how the description can be adapted to measure other analytes (such as cholesterol, ketone bodies, alcohol, etc.) in other fluids (such as saliva, urine, interstitial fluid, etc.)
  • [0032]
    The electrochemical (amperometric) method for measuring an analyte concentration in an aqueous sample involves placing the sample into an electrochemical cell that has at least two electrodes and an impedance that is suitable for the amperometric measurement. The analyte is allowed to react directly with an electrode or with a redox reagent to form an oxidizable (or reducible) substance in an amount that corresponds to the analyte concentration. The quantity of oxidizable (or reducible) substance is then determined electrochemically.
  • [0033]
    In order to obtain an accurate measurement of the substance, it is important to assure that sufficient sample is provided to the cell. For example, if the sample is insufficient, it can reduce the effective electrode area and give an incorrect result. Assurance that the sample is sufficient is provided by the device of this invention, shown in FIG. 1.
  • [0034]
    [0034]FIG. 1 is an exploded view of an embodiment of multilayer device 10. Top insulating sheet 12 has an electrically conductive surface 14, which is typically a metal, plated on a surface of insulating sheet 12 by vacuum deposition, sputtering, electroplating, or any other suitable method for providing a conductive surface, well known in the art. In from a longitudinal edge of surface 14 is scored insulating line 16. Scored line 16 extends through the thickness of surface 14, to provide a gap in the conductive path across the width of the device.
  • [0035]
    Intermediate insulating layer 18 is sandwiched between conductive surface 14 of top insulating sheet 12 and conductive surface 20 of bottom insulating sheet 22. Intermediate layer 18 is preferably a thermoplastic sheet with adhesive on both surfaces for adhering to sheets 12 and 22. Conductive surface 20 is typically a metal plated on sheet 22 by one of the methods mentioned earlier. Cutout 30 in intermediate layer 18 provides—between conductive-coated sheets 12 and 22—inlet 32, outlet 34, and the electrochemical cell 36 that lies between the inlet and outlet. An optional serration 17 in scored line 16 enhances flow from inlet 32 to outlet 34, by a mechanism that is described later. Within channel 30, a dry reagent, consisting of buffer, mediator, and enzyme, is deposited on conductive surface 20 and/or, 14. Electrochemical cell 36 is the region within which is measured an electrical parameter of the fluid/reagent combination. The region in which the reagent is coated generally, but not necessarily, corresponds to the cell 36. For simplicity, that correspondence is assumed in the description below. The reagent and electrochemical cell 36 may be limited to the region between insulating line 16 and the inlet 32. Alternatively, the reagent coating (and cell) may extend over the entire cutout region between the edges of the device.
  • [0036]
    [0036]FIG. 2 is a plan view of another embodiment of the device of FIG. 1. The device 10′ of FIG. 2 includes a second scored line 16A, in from the other longitudinal edge of conductive surface 14. Thus, the device of FIG. 2 is symmetrical, so that the sample can be admitted from either edge; i.e., there is no distinction between inlet and outlet. Score lines 16 and 16A divide conductive surface 14 into three regions—14A, 14B, and 14C—each insulated from the other two. As is clear in FIG. 2, score lines 16 and 16A have serrations 40 and 40A, respectively, that form angles, whose vertices “point” to both edges of the device. The serrations are provided to enhance flow through channel 30 in both directions, as described below.
  • [0037]
    When fluid flows through a capillary channel, such as channel 30, a discontinuity in channel cross section can form a “stop junction,” which can stop the fluid flow, as described in U.S. Pat. Nos. 4,426,451; 5,230,866; and 5,912,134, incorporated herein by reference. Score lines 16 and 16A create such cross section discontinuities. The stop junction results from surface tension that creates a back pressure that stops the fluid meniscus from proceeding through the discontinuity. The stop junction is weakened, and flow thereby enhanced, when the leading edge of the meniscus encounters the vertex of an acute angle and is then stretched along the arms of the angle. This may be described as the angle “pointing” in a direction opposite to the direction of fluid flow. This process may be better understood by reference to FIGS. 3A, 3B, and 3C.
  • [0038]
    [0038]FIG. 3A depicts the action of the stop junction when the serration is absent. The fluid (flowing left-to-right in the figure) is stopped at score line 16. A serration in score line 16 (such as serration 17 in FIG. 1) serves to weaken the stop junction and facilitates flow through the scored region. Although serration 17 would weaken the stop junction, and thereby facilitate fluid flow in both directions through capillary channel 30, the effect is not the same for both directions.
  • [0039]
    [0039]FIGS. 3B and 3C show the fluid before and just after the meniscus breaks through a stop junction having serrations whose vertices point in opposite directions (like that of FIG. 2). Note that the breakthrough occurs first at the vertex that “points” opposite to the direction of fluid flow. The effectiveness of the serration in enhancing flow through a stop junction in a capillary channel depends on the angle and the length of the legs that form the angle. The smaller the angle and the longer the legs, the greater the effectiveness of the serration. Thus, if the angle is small and the legs long, only a small hydraulic pressure differential across the scored region will cause the sample to flow through it.
  • [0040]
    [0040]FIG. 4 is a cross section along the line 4-4 of FIG. 2. As is clear from FIG. 4, score lines 16 and 16A interrupt conductive surface 14 and extend into insulating sheet 12. Conductive surface 14 is typically gold, and conductive surface 20 is typically palladium, but each may alternatively be any other conductive material that does not react with the reagent or sample and that can be applied to an insulating surface. Suitable materials include tin oxide, platinum, iridium, stainless steel, and carbon. The thickness of the coating should at least be sufficient to provide adequate. conductivity—generally, about. 10 ohms per square, or less. Gold coatings are typically about 10-20 nm, palladium typically about 20-40 nm. The conductive coatings preferably have a hydrophilic overcoating to enhance filling when channel 30 is a capillary channel. The overcoat must adhere to the conductive coating but not react with the sample fluid. Insulating sheets 12 and 22 may be any suitable thermoplastic sheet, such as polyester, polyethylene, polypropylene, polycarbonate, polyimide, etc. Polyester of about 0.2 mm thickness is suitable and inexpensive.
  • [0041]
    As seen in FIGS. 4 and 5, a fill-detection device 10′ of the type shown in FIG. 2 has available four discrete electrodes—14A, 14B, 14C, and 20. Thus, an electrochemical meter could, in principle, measure the current or voltage output across six different electrode pairs—14A, 20; 14B, 20; 14C, 20; 14A, 14B; 14B, 14C; and 14A, 14C. In a preferred embodiment, a meter periodically (e.g., every 0.1 second) measures the voltages (at constant current) across 14A, 20 and 14C, 20. In that way, the meter detects sample entry and identifies which side of channel 30 the sample is entering. For example, if (conductive) sample enters at the left edge, as shown in FIGS. 4 and 5, voltage 14A, 20 drops. Thereafter, a drop in any of 14A, 14C; 14B, 14C.; or 14C, 20 voltages signals that the volume of channel 30 is filled between 16 and 16A. If the time to fill under normal conditions is known, simple error trapping allows a strip to be rejected if fill time exceeds a preset maximum. Similarly, if sample enters at the right edge, voltage 14C, 20 drops, and a drop in 14A, 14C; 14A, 14B, or 14A, 20 signals that channel 30 is filled (at least beyond the serration most distant from the sample entry).
  • [0042]
    Instead of, or in addition to, monitoring 14A, 20 and 14C, 20 to detect sample entry, 14A, 14B, and 14B, 14C could be monitored to detect partial filling of channel 30. The time at which the cell has filled is determined as described above.
  • [0043]
    Another alternative for monitoring partial filling is to just measure voltage 14B, 20. That alternative requires less switching and simple controls. By not requiring very rapid switching there may also be a cost saving. The time at which the cell has filled is then signaled by a drop in voltage 14A, 14C. More generally, changes in current or voltage across one or more of the pairs can be used to track the progress of sample into and through the cell. Of course, if only a single score is used (as shown in FIG. 1), there are only three discrete electrodes, and the monitoring options are correspondingly reduced. Inversely, if surface 20 is scored, instead of or in addition to 14, sample flow can be monitored using other, or additional, voltage pairs.
  • [0044]
    [0044]FIG. 5 depicts a block diagram that shows circuitry which can accomplish the fill detection described above. Initially, a constant current source (101) is applied to one of the electrode pair combinations, using switches 105 and 106. With no sample on the strip, the resistances between all six electrode pairs are very large, and the current passing through the strip is negligible. The output voltage of voltage buffer 104(V) is high in this condition. When sample bridges the gap of a monitored pair, the resistance and voltage drop significantly. V then is fed to microcontroller 104 through analog-to-digital converter 103. Microcontroller 104, recognizing this reduced voltage as sample detection, switches 105 and 106 to probe one of the other electrode pairs, to confirm that the cell is filled.
  • [0045]
    [0045]FIG. 6 is an exploded perspective view of an alternative embodiment of a device of this invention, in which sample is applied to the end, rather than side, of the device. Top layer 112 has a coating 114 of a conductor, such as gold, on its underside. The coating has insulating line 116 scored into the surface, and serration 140 in score line 116 facilitates flow of sample into channel 130 of insulating layer 118. Bottom layer 122 has a coating 120 of palladium, or other conductor. Electrical contact with coating 120 is made through access hole 142 in top layer 112 and gap 144 in insulating layer 118. Electrical contact with coating 114 is made through openings 146 in bottom layer 122 and gap 144 in insulating layer 118. Electrochemical cell 136 is formed by channel 130 and the metal-coated top and bottom layers. Note that after the device is assembled, through-hole 148 is punched through all three layers to provide a vent in top layer 112 to permit filling of channel 130 and to provide a stop junction at the distal end of the channel (where hole 148 is cut into insulating layer 118). At the same time, the proximal (open) end of the channel is cut, together with the adjoining ends of layers 112 and 122. The two cuts, made simultaneously in the assembled layers, provide accurate and reproducible channel length, which in turn permits accurate and reproducible measurements.
  • [0046]
    A device of the type described above can use a glucose oxidase (GOD)/ferricyanide system to determine glucose concentrations via the following reactions, in which GOD* is the reduced enzyme.
  • glucose+GOD→gluconic acid+GOD*   Reaction 1
  • GOD*+2ferricyanide→GOD+2ferrocyanide.   Reaction 2
  • [0047]
    Ferricyanide ([Fe(CN)6]3−) is the mediator, which returns the GOD* to its catalytic state. GOD, an enzyme catalyst, will continue to oxidize glucose so long as excess mediator is present. Ferrocyanide ([Fe(CN)6]4−) is the product of the total reaction. Ideally, there is no ferrocyanide initially, although in practice there is often a small quantity. After the reaction is complete, the concentration of ferrocyanide (measured electrochemically) indicates the initial concentration of glucose. The total reaction, reaction 3, is the sum of reactions 1 and 2.
  • [0048]
    “Glucose” refers specifically to β-D-glucose.
  • [0049]
    Details of this system are described in U.S. Pat. No. 5,942,102, issued on Aug. 24, 1999, and incorporated herein by reference.
  • [0050]
    A second embodiment of the present invention is a method for providing an electrically-conductive pattern on a conductive-coated flexible insulator, such as sheet 12 of FIG. 2. An apparatus for preparing a pattern such as that designated 16 and 16A in conductive coating 14 is depicted in FIG. 7.
  • [0051]
    As shown in FIG. 7, web 42, comprising conductive coating 44 on flexible insulator 46 passes between anvil 48 and cutting die 50 to score selected areas of coating 44. The knife regions of die 50 are raised a height h, greater than the thickness of coating 44, so that the cut areas become insulating regions in the coating. However, the knife regions should not be raised so high that the mechanical strength of insulator 46 is undermined. Preferably, the knife height h is about one thousand to ten thousand times the thickness of coating 44, depending on the uniformity and precision of the tooling for cutting. Preferably, as shown, anvil 48 and cutting die 50 are rollers that the web passes between.
  • [0052]
    Alternative methods for providing a pattern of score lines in a conductive coating will be apparent to a person of ordinary skill in the art. For example, if insulator 46 is deformable, then standard relief patterning methods can be used, such as those used in microreplication. (See, e.g., U.S. Pat. Nos. 5,642,015; 5,514,120; and 5,728,446.)
  • [0053]
    It will be understood by those skilled in the art that the foregoing descriptions are illustrative of practicing the present invention, but are in no way limiting. Variations of the detail presented herein may be made, without departing from the scope and spirit of the present invention.
Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US3256170 *25 May 196414 Jun 1966Mercil Plating Equipment CompaElectroplating barrel
US3547724 *7 Feb 196715 Dec 1970Rogers CorpMethod of and apparatus for producing printed circuits
US3713944 *28 May 197030 Jan 1973Essex International IncA method of manufacture of printed circuits by die stamping
US4244125 *7 Mar 197913 Jan 1981Corey Jan JLabel system for making integrated circuit diagrams and printed circuit boards
US4545382 *22 Oct 19828 Oct 1985Genetics International, Inc.Sensor for components of a liquid mixture
US4940945 *2 Nov 198710 Jul 1990Biologix Inc.Interface circuit for use in a portable blood chemistry measuring apparatus
US5264103 *15 Oct 199223 Nov 1993Matsushita Electric Industrial Co., Ltd.Biosensor and a method for measuring a concentration of a substrate in a sample
US5366609 *8 Jun 199322 Nov 1994Boehringer Mannheim CorporationBiosensing meter with pluggable memory key
US5437999 *22 Feb 19941 Aug 1995Boehringer Mannheim CorporationElectrochemical sensor
US5514120 *22 Aug 19937 May 1996Minnesota Mining And Manufacturing CompanyLiquid management member for absorbent articles
US5642015 *1 May 199524 Jun 1997The University Of British ColumbiaElastomeric micro electro mechanical systems
US5798031 *12 May 199725 Aug 1998Bayer CorporationElectrochemical biosensor
US5997817 *5 Dec 19977 Dec 1999Roche Diagnostics CorporationElectrochemical biosensor test strip
US6004441 *10 Jul 199721 Dec 1999Matsushita Electric Industrial Co., Ltd.Biosensor
US6071391 *15 Dec 19976 Jun 2000Nok CorporationEnzyme electrode structure
US6156173 *18 Jan 20005 Dec 2000Nok CorporationEnzyme electrode structure
US6212417 *20 Aug 19993 Apr 2001Matsushita Electric Industrial Co., Ltd.Biosensor
US6252822 *28 Feb 200026 Jun 2001The United States Of America As Represented By The Secretary Of The NavyCountermeasure device with air bag hover system and pressure compensated acoustic projectors
US6287451 *2 Jun 199911 Sep 2001Handani WinartaDisposable sensor and method of making
US6299757 *6 Oct 19999 Oct 2001Therasense, Inc.Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US8532731 *8 May 200910 Sep 2013Abbott Diabetes Care Inc.Methods of determining analyte concentration
US879517630 Jul 20075 Aug 2014Abbott Diabetes Care Inc.Integrated sample acquisition and analyte measurement device
US90172595 Aug 201428 Apr 2015Abbott Diabetes Care Inc.Integrated sample acquisition and analyte measurement device
US923486319 May 201412 Jan 2016Abbott Diabetes Care Inc.Small volume in vitro analyte sensor
US923486419 Aug 201412 Jan 2016Abbott Diabetes Care Inc.Small volume in vitro analyte sensor
US927166927 Apr 20151 Mar 2016Abbott Diabetes Care Inc.Method for integrated sample acquisition and analyte measurement device
US929159219 May 201422 Mar 2016Abbott Diabetes Care Inc.Small volume in vitro analyte sensor
US931660919 May 201419 Apr 2016Abbott Diabetes Care Inc.Small volume in vitro analyte sensor
US934159119 May 201417 May 2016Abbott Diabetes Care Inc.Small volume in vitro analyte sensor
US966205717 Feb 201630 May 2017Abbott Diabetes Care Inc.Integrated sample acquisition and analyte measurement method
US20060247509 *28 Apr 20062 Nov 2006Tuccillo Mark JECG cable for use in MRI
US20090270764 *8 May 200929 Oct 2009Abbott Diabetes Care Inc.Methods of determining concentration of ketone bodies
Classifications
U.S. Classification600/345
International ClassificationG01N33/487, G01N27/416, G01N27/49, G01N33/483, G01N27/327
Cooperative ClassificationG01N27/3272
European ClassificationG01N27/404B, G01N27/327B
Legal Events
DateCodeEventDescription
3 Oct 2003ASAssignment
Owner name: LIFESCAN, INC., CALIFORNIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:SHARTLE, ROBERT JUSTICE;OHARA, TIMOTHY J.;KERMANI, MAHYAR Z.;REEL/FRAME:014584/0779
Effective date: 20000331