EP1128200A3 - Microscope construction - Google Patents
Microscope construction Download PDFInfo
- Publication number
- EP1128200A3 EP1128200A3 EP00123968A EP00123968A EP1128200A3 EP 1128200 A3 EP1128200 A3 EP 1128200A3 EP 00123968 A EP00123968 A EP 00123968A EP 00123968 A EP00123968 A EP 00123968A EP 1128200 A3 EP1128200 A3 EP 1128200A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- light
- fluorescent
- detection
- transmission light
- transmission
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
Abstract
Ein Mikroskop-Aufbau, insbesondere für die konfokale Scan-Mikroskopie, mit einer Lichtquelle (1) zur Beleuchtung eines zu untersuchenden Objekts (6) und mindestens einem Fluoreszenzlicht-Detektor (11, 14) zur Detektion von in dem Objekt (6) erzeugtem Fluoreszenzlicht (10, 13) und mindestens einem Transmissionslicht-Detektor (16) zur Detektion von durch das Objekt (6) tretendem Transmissionslicht (15) ist im Hinblick auf eine sichere Durchführung unterschiedlichster Experimente mit jeweils hohem Detektionsgrad derart ausgestaltet und weitergebildet, dass die Fluoreszenzlicht- und Transmissionslicht-Detektoren (11, 14; 16) derart angeordnet sind, dass eine simultane Detektion von Fluoreszenz- und Transmissionslicht (10, 13; 15) ermöglicht ist. A microscope assembly, in particular for confocal scanning microscopy, with a light source (1) for illuminating an object (6) to be examined and at least one fluorescent light detector (11, 14) for detecting fluorescent light generated in the object (6) (10, 13) and at least one transmission light detector (16) for the detection of transmission light (15) passing through the object (6) is designed and developed with a view to reliably carrying out a wide variety of experiments, each with a high degree of detection, such that the fluorescent light and transmission light detectors (11, 14; 16) are arranged in such a way that a simultaneous detection of fluorescent and transmission light (10, 13; 15) is made possible.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10003570A DE10003570A1 (en) | 2000-01-27 | 2000-01-27 | Microscope setup |
DE10003570 | 2000-01-27 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1128200A2 EP1128200A2 (en) | 2001-08-29 |
EP1128200A3 true EP1128200A3 (en) | 2002-05-15 |
Family
ID=7628932
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00123968A Ceased EP1128200A3 (en) | 2000-01-27 | 2000-11-03 | Microscope construction |
Country Status (4)
Country | Link |
---|---|
US (1) | US6831780B2 (en) |
EP (1) | EP1128200A3 (en) |
JP (1) | JP5162781B2 (en) |
DE (1) | DE10003570A1 (en) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4804665B2 (en) * | 2001-08-09 | 2011-11-02 | オリンパス株式会社 | Laser microscope |
US6888148B2 (en) * | 2001-12-10 | 2005-05-03 | Carl Zeiss Jena Gmbh | Arrangement for the optical capture of excited and /or back scattered light beam in a sample |
DE10314750A1 (en) * | 2003-03-31 | 2004-11-04 | Leica Microsystems Heidelberg Gmbh | Scanning microscope for biological applications has an objective with a contrast device which enables use of the microscope in a Hoffman-modulation contrast mode |
JP4647641B2 (en) * | 2003-04-14 | 2011-03-09 | ナノフォトン株式会社 | Laser microscope |
DE10332064A1 (en) * | 2003-07-11 | 2005-01-27 | Carl Zeiss Jena Gmbh | Arrangement for detecting the illumination radiation in a laser scanning microscope |
DE102004029733B4 (en) | 2003-07-26 | 2022-03-31 | Leica Microsystems Cms Gmbh | Scanning microscope and method for scanning microscopy |
US20050017197A1 (en) * | 2003-07-26 | 2005-01-27 | Leica Microsystems Heidelberg Gmbh | Scanning microscope and method for scanning microscopy |
US6961126B2 (en) * | 2003-10-23 | 2005-11-01 | Honeywell International Inc. | Optical wavelength splitter |
JP4954452B2 (en) * | 2004-07-06 | 2012-06-13 | オリンパス株式会社 | microscope |
DE102004034971A1 (en) | 2004-07-16 | 2006-02-09 | Carl Zeiss Jena Gmbh | Scanning microscope with linear scanning and use |
DE102004034976A1 (en) | 2004-07-16 | 2006-02-16 | Carl Zeiss Jena Gmbh | Scanning microscope and use |
EP1710609A1 (en) * | 2005-04-08 | 2006-10-11 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Optical scanning device and method of deriving same |
JP4512698B2 (en) * | 2005-08-30 | 2010-07-28 | ナノフォトン株式会社 | Laser microscope |
US7924432B2 (en) * | 2006-12-21 | 2011-04-12 | Howard Hughes Medical Institute | Three-dimensional interferometric microscopy |
US7916304B2 (en) | 2006-12-21 | 2011-03-29 | Howard Hughes Medical Institute | Systems and methods for 3-dimensional interferometric microscopy |
DE102007047468A1 (en) * | 2007-09-28 | 2009-04-02 | Carl Zeiss Microimaging Gmbh | Method and arrangement for optically detecting an illuminated sample |
JP5038094B2 (en) * | 2007-10-31 | 2012-10-03 | オリンパス株式会社 | Laser scanning microscope |
FI20115999A0 (en) * | 2011-10-11 | 2011-10-11 | Teknologian Tutkimuskeskus Vtt Oy | Optical measurement |
JP6014449B2 (en) * | 2012-10-02 | 2016-10-25 | アストロデザイン株式会社 | Laser scanning microscope equipment |
JP6243110B2 (en) * | 2012-10-15 | 2017-12-06 | アストロデザイン株式会社 | Laser scanning microscope equipment |
JP2015127738A (en) * | 2013-12-27 | 2015-07-09 | ソニー株式会社 | Microscope system and control method |
WO2015163261A1 (en) * | 2014-04-24 | 2015-10-29 | オリンパス株式会社 | Microscope and microscopic observation method |
WO2017046863A1 (en) | 2015-09-15 | 2017-03-23 | オリンパス株式会社 | Microscope and microscope observation method |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3742806A1 (en) * | 1987-12-17 | 1989-07-13 | Zeiss Carl Fa | Method and device for producing fluorescence images |
WO1994018593A1 (en) * | 1993-02-12 | 1994-08-18 | Medical Research Council | Detecting means for a scanning optical microscope |
US5420717A (en) * | 1992-02-18 | 1995-05-30 | Olympus Optical Co., Ltd. | Adjustable-contrast microscope |
US5796112A (en) * | 1993-06-03 | 1998-08-18 | Hamamatsu Photonics K.K. | Laser scanning optical system and laser scanning optical apparatus |
US5945669A (en) * | 1996-08-27 | 1999-08-31 | Olympus Optical Co., Ltd. | Laser scan microscope and light-measuring apparatus |
DE19902625A1 (en) * | 1998-01-28 | 1999-09-30 | Leica Microsystems | Device for simultaneous detection of several spectral ranges of a light beam, such as that used with a laser scanner |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1215954B (en) * | 1963-02-08 | 1966-05-05 | Leitz Ernst Gmbh | Photometers for observation instruments, especially microscopes |
JPS63306413A (en) * | 1987-06-09 | 1988-12-14 | Olympus Optical Co Ltd | Scanning type optical microscope |
JPH0527177A (en) * | 1991-07-25 | 1993-02-05 | Fuji Photo Film Co Ltd | Scanning type microscope |
US5535052A (en) * | 1992-07-24 | 1996-07-09 | Carl-Zeiss-Stiftung | Laser microscope |
US5874726A (en) * | 1995-10-10 | 1999-02-23 | Iowa State University Research Foundation | Probe-type near-field confocal having feedback for adjusting probe distance |
-
2000
- 2000-01-27 DE DE10003570A patent/DE10003570A1/en not_active Withdrawn
- 2000-11-03 EP EP00123968A patent/EP1128200A3/en not_active Ceased
-
2001
- 2001-01-12 US US09/759,509 patent/US6831780B2/en not_active Expired - Lifetime
- 2001-01-16 JP JP2001007783A patent/JP5162781B2/en not_active Expired - Lifetime
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3742806A1 (en) * | 1987-12-17 | 1989-07-13 | Zeiss Carl Fa | Method and device for producing fluorescence images |
US5420717A (en) * | 1992-02-18 | 1995-05-30 | Olympus Optical Co., Ltd. | Adjustable-contrast microscope |
WO1994018593A1 (en) * | 1993-02-12 | 1994-08-18 | Medical Research Council | Detecting means for a scanning optical microscope |
US5796112A (en) * | 1993-06-03 | 1998-08-18 | Hamamatsu Photonics K.K. | Laser scanning optical system and laser scanning optical apparatus |
US5945669A (en) * | 1996-08-27 | 1999-08-31 | Olympus Optical Co., Ltd. | Laser scan microscope and light-measuring apparatus |
DE19902625A1 (en) * | 1998-01-28 | 1999-09-30 | Leica Microsystems | Device for simultaneous detection of several spectral ranges of a light beam, such as that used with a laser scanner |
Also Published As
Publication number | Publication date |
---|---|
JP5162781B2 (en) | 2013-03-13 |
US20010012151A1 (en) | 2001-08-09 |
EP1128200A2 (en) | 2001-08-29 |
JP2001235683A (en) | 2001-08-31 |
US6831780B2 (en) | 2004-12-14 |
DE10003570A1 (en) | 2001-08-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR |
|
AX | Request for extension of the european patent |
Free format text: AL;LT;LV;MK;RO;SI |
|
RTI1 | Title (correction) |
Free format text: MICROSCOPE CONSTRUCTION |
|
PUAL | Search report despatched |
Free format text: ORIGINAL CODE: 0009013 |
|
AK | Designated contracting states |
Kind code of ref document: A3 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR |
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AX | Request for extension of the european patent |
Free format text: AL;LT;LV;MK;RO;SI |
|
RIC1 | Information provided on ipc code assigned before grant |
Free format text: 7G 02B 21/24 A, 7G 02B 21/00 B, 7G 02B 21/16 B |
|
17P | Request for examination filed |
Effective date: 20020928 |
|
17Q | First examination report despatched |
Effective date: 20021126 |
|
AKX | Designation fees paid |
Designated state(s): CH DE FR GB LI |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED |
|
18R | Application refused |
Effective date: 20050724 |