EP1045248A3 - Luminescent labeled immunoassay method and analysis element therefor - Google Patents

Luminescent labeled immunoassay method and analysis element therefor Download PDF

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Publication number
EP1045248A3
EP1045248A3 EP00107951A EP00107951A EP1045248A3 EP 1045248 A3 EP1045248 A3 EP 1045248A3 EP 00107951 A EP00107951 A EP 00107951A EP 00107951 A EP00107951 A EP 00107951A EP 1045248 A3 EP1045248 A3 EP 1045248A3
Authority
EP
European Patent Office
Prior art keywords
antibody
enzyme
membrane
immunoassay method
analysis element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP00107951A
Other languages
German (de)
French (fr)
Other versions
EP1045248A2 (en
Inventor
Hiroshi c/o Fuji Photo Film Co. Ltd. Shinoki
Osamu C/O Fuji Photo Film Co. Ltd. Seshimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Publication of EP1045248A2 publication Critical patent/EP1045248A2/en
Publication of EP1045248A3 publication Critical patent/EP1045248A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/02—Hepadnaviridae, e.g. hepatitis B virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae

Abstract

A liquid sample containing an analyte antibody and a solution containing an enzyme-labeled second antibody are fed successively to a microporous membrane on which an antigen is immobilized, to form a complex of the immobilized antigen, the antibody and the enzyme-labeled second antibody. Thereafter, an enzyme substrate is fed to the membrane to cause a luminous reaction on the membrane. The existence and the quantity of the antibody are detected based on the luminous quantity. The use of chemiluminescence enables to detect the antibody at extremely high sensitivity. When the luminescence is detected by means of a photography film, adjustment of the exposure time enables to set up the sensitivity at any magnitude. Especially, use of an instant photography film can provide an extremely convenient and rapid detection of the antibody.
EP00107951A 1999-04-16 2000-04-17 Luminescent labeled immunoassay method and analysis element therefor Ceased EP1045248A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP10906899 1999-04-16
JP11109068A JP2000304750A (en) 1999-04-16 1999-04-16 Luminous label immunoassay and its analysis element

Publications (2)

Publication Number Publication Date
EP1045248A2 EP1045248A2 (en) 2000-10-18
EP1045248A3 true EP1045248A3 (en) 2000-12-13

Family

ID=14500793

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00107951A Ceased EP1045248A3 (en) 1999-04-16 2000-04-17 Luminescent labeled immunoassay method and analysis element therefor

Country Status (2)

Country Link
EP (1) EP1045248A3 (en)
JP (1) JP2000304750A (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004051268A1 (en) * 2002-12-03 2004-06-17 Celltech R & D Limited Assay for identifying antibody producing cells
ES2360369T5 (en) * 2004-12-23 2021-12-13 Hoffmann La Roche Detection of a therapeutic antibody in an experimental animal
US8012770B2 (en) 2009-07-31 2011-09-06 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
EP2486120B1 (en) 2009-10-09 2014-04-02 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
EP3608021A3 (en) 2011-01-27 2020-04-22 Invisible Sentinel, Inc. Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof
WO2013134503A2 (en) 2012-03-09 2013-09-12 Invisible Sentinel, Inc. Methods And Compositions For Detecting Multiple Analytes With A Single Signal
CN103616511A (en) * 2013-12-06 2014-03-05 苏州长光华医生物医学工程有限公司 Hepatitis B core antibody measurement kit and detection method thereof
KR102351951B1 (en) * 2020-05-06 2022-01-17 한국원자력연구원 Method for detecting mycobacterium tuberculosis

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4472498A (en) * 1981-07-24 1984-09-18 Fuji Photo Film Co., Ltd. Analysis film and a method of analysis using the same
US4966856A (en) * 1985-06-19 1990-10-30 Konishiroku Photo Industry Co., Ltd. Analytical element and the analytical method using the element
EP0420170A2 (en) * 1989-09-28 1991-04-03 Becton, Dickinson and Company Stabilizing reagent for membrane immunoassay
EP0512390A1 (en) * 1991-05-02 1992-11-11 FUJIREBIO Inc. Test strip for immunoassay, assay method using the same and immunoassay device
WO1995023969A1 (en) * 1994-03-04 1995-09-08 Aquaculture Diagnostics Limited Rapid immunological test

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4472498A (en) * 1981-07-24 1984-09-18 Fuji Photo Film Co., Ltd. Analysis film and a method of analysis using the same
US4966856A (en) * 1985-06-19 1990-10-30 Konishiroku Photo Industry Co., Ltd. Analytical element and the analytical method using the element
EP0420170A2 (en) * 1989-09-28 1991-04-03 Becton, Dickinson and Company Stabilizing reagent for membrane immunoassay
EP0512390A1 (en) * 1991-05-02 1992-11-11 FUJIREBIO Inc. Test strip for immunoassay, assay method using the same and immunoassay device
WO1995023969A1 (en) * 1994-03-04 1995-09-08 Aquaculture Diagnostics Limited Rapid immunological test

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ROITT, I; BROSTOFF, J; MALE, D: "Immunology", GOWER MEDICAL PUBLISHING, LONDON, XP002149631 *

Also Published As

Publication number Publication date
EP1045248A2 (en) 2000-10-18
JP2000304750A (en) 2000-11-02

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