DE79139T1 - Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer. - Google Patents

Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer.

Info

Publication number
DE79139T1
DE79139T1 DE198282305489T DE82305489T DE79139T1 DE 79139 T1 DE79139 T1 DE 79139T1 DE 198282305489 T DE198282305489 T DE 198282305489T DE 82305489 T DE82305489 T DE 82305489T DE 79139 T1 DE79139 T1 DE 79139T1
Authority
DE
Germany
Prior art keywords
nucleic acid
microorganisms
pair
reagents
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282305489T
Other languages
English (en)
Inventor
Tuula Marjut Fi-02660 Espoo 66 Ranki
Hans Erik Fi-02940 Espoo 94 Soederlund
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ORION CORP Ltd 00510 HELSINKI FI
Original Assignee
ORION CORP Ltd 00510 HELSINKI FI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ORION CORP Ltd 00510 HELSINKI FI filed Critical ORION CORP Ltd 00510 HELSINKI FI
Publication of DE79139T1 publication Critical patent/DE79139T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)

Claims (12)

Ansprüche
1. Anwendung der Sandwich-Hybridisierungstechnik fUr Nukleinsäuren, wobei diese Technik darin besteht, dass man einsträngige NukLeinsäure aus einem Mikroorganismus mit einem Paar verschiedener Nukleinsäurereagenzien in Berührung bringt, wobei beide Reagenzien des Paars einsträngig und mit der vom Mikroorganismus abgeleiteten NukLeinsäure komplementär sind und ein Glied des Paars ein an einen festen Träger gebundenes NukIeinsäurefragment ist, während das andere ein mit einem Indikator markiertes Nukleinsäurefragment darstellt, wodurch sich ein an den festen Träger gebundenes markiertes Hybrid bildet, auf die Identifizierung eines in einer Probe vorliegenden Mikroorganismus bzw. einer Gruppe von Mikroorganismen, wobei das Paar NukLeinsäurereagenzien entsprechend dem Mikroorganismus oder der Gruppe von Mikroorganismen, deren Gegenwart in der Probe zu erwarten ist, ausgewählt und die Richtigkeit der Identifizierung durch Erfassung des Ausmasses der Bildung eines an den festen Träger gebundenen markierten Hybrids nachgewiesen wird.
2. Anwendung der Sandwich-Hybridisierungstechnik für Nukleinsäure nach Anspruch 1, dadurch gekennzeichnet, dass man mehrere NukLeinsäurereagenzienpaare verwendet, wobei jedes Paar mit von einem verschiedenen Mikroorganismus bzw. einer Gruppe von Mikroorganismen, wovon irgendeiner in der Probe zu erwarten ist, abgeleiteten Nukleinsäure komplementär ist.
3. Anwendung der Sandwich-Hybridisierungstechnik für Nukleinsäure nach Anspruch 2, dadurch gekennzeichnet, dass mehrere verschiedene Mikroorganismen bzw. Gruppen von Mikroorganismen in der Probe vorliegen und in dersel-
0079 Ί
ben ungeteiLten Probe durch Anwendung einer Reihe verschiedener Paare von NukIeinsäurereagenzien, die mit Nukleinsäuren aus den verschiedenen Mikroorganismen bzw. Gruppen von Mikroorganismen, deren Gegenwart in der Probe zu erwarten ist, komplementär sind /nachgewiesen werden.
4. Methode zu Identifizierung von in einer Probe vorliegenden Mikroorganismen bzw. Gruppen von Mikroorganismen durch Sandwich-Hybridisierung von Nukleinsäuren unter Verwendung eines Paars völlig verschiedener Nukleinsäurereagenzien, die beide mit Nukleinsäure aus demselben bekannten Mikroorganismus bzw. Gruppe vr>n Mikroorganismen komplementär und damit hybridisierbar sind, wobei ein Glied des Paars von NukLeinsäurereagenzien ein an einen festen Träger gebundenes einsträngiges NukLeinsäurefragment und das andere ein mit einem Indikator markiertes einsträngiges Nukleinsäurefragment ist, wodurch sich bei gelungener Hybridisierung ein an den festen Träger gebundenes markiertes Hybrid biLdet, dadurch gekennzeichnet, dass mehrere in einer einzigen ungeteiLten Probe vorhandene Mikroorganismen bzw. Gruppen von Mikroorganismen identifiziert werden, indem man Nukleinsäuren aus den Mikroorganismen in der Probe, nachdem sie einsträngig gemacht wurden, mit mehreren der Paare völlig verschiedener NukLeinsäurereagentien in Berührung bringt, wobei die Reagenzien in unterschiedlichen Paaren mit Nukleinsäure aus verschiedenen bekannten Mikroorganismen bzw. Gruppen von Mikroorganismen komplementär und damit hybridisierbar sind, und die Bildung oder Nichtbildung an einen festen Träger gebundener markierter Hybride fUr jedes der Paare von NukLeinsäurereagenzien nachweist.
5. Reagenzkombination zur Verwendung nach Anspruch 2 oder 3 bzw. fUr die Methode nach Anspruch 4, gekennzeichnet durch die Gegenwart von mehreren Paaren verschiedener NukLeinsäurereagenzien, wobei die beiden Reagenzien desselben Paars jeweils mit der NukLeinsäure aus demselben Mikroorganismus bzw. Gruppe von Mikroorganismen komplementär und damit hybridisierbar sind und die Reagenzien unterschiedlicher Paare mit NukLeinsäure aus unter-
schied I ichen Mikroorganismen bzw. Gruppen von Mikroorganismen komplementär und damit hybridisierbar sind, wobei ein Reagenz, aus jedem Paar ein an einen festen Träger gebundenes einsträngiges NukLeinsäurefragment und das andere Reagenz in jedem Paar ein mit einem Indikator markiertes einstrangiges Nuk I einsäurefragment ist. 6. Reagenzkombination nach Anspruch 5, dadurch gekennzeichnet, dass die beiden NukI einsäurereagenzien im selben Paar jeweils entweder direkt aus dem Genom des Mikroorganismus, mit dem sie komplementär sind, oder durch eine DNA-Rekombinationstechnik und nachherige Einsträngigmachung hergestellte Fragmente sind sowie eines der Reagenzien an einen festen Träger gebunden und das andere mit einem Indikator markiert ist.
7. Reagenzkombination nach Anspruch 5 oder 6, dadurch gekennzeichnet, dass eines oder mehrere der Paare Nukleinsäure reagenz i en mit Nukleinsäure aus einem fUr eine Infektion der Atemwege oder Durchfall verantwortlichen B a k terium oder Virus, einem fUr eine Geschlechtskrankheit verantwortlichen Bakterium, Virus, einer Hefe oder einem Protozoon oder einem für Sepsis oder unzureichende Lebensmittelhygiene verantwortlichen Bakterium komplementär und damit hybridisierbar sind.
8. Reagenzkombination nach Anspruch 5 oder 6, dadurch 5 gekennzeichnet, dass eines der Paare Nukleinsäurereagenzien mit Nukleinsäure aus Adenovirus komplementär und damit hybridisierbar ist, wobei dieses Paar aus dem an einen festen Träger gebundenen Adenovirusrekombinationsp lasmid Ad_DpBR322 und dem mit einem Indikator markierten Adenovirus-Ad -BamHI-C-Fragment besteht.
9. Reagenzkombination nach einem der Ansprüche 5 bis 8, dadurch gekennzeichnet, dass eines der Paare Nukleinsäurereagenzien mit Nukleinsäure aus Semliki-Forest-Virus komplementär und damit hybridisierbar ist, wobei dieses Paar aus dem an einen festen Träger gebundenen EcoRI-XhoI-Fragment A des pKTH312-Plasmids und dem mit einem Indikator markierten EcoRI-Xhol-Fragment B des pKTH312-Plasmids besteht.
10." Reagenzkombination nach einem der Ansprüche 5 bis
00791339, dadurch gekennzeichnet, dass eines der Paare Nukleinsäurereagenzoen mit Nukleinsäure aus SV40-Virus komplementär und damit hybridisierbar ist, wobei dieses Paar aus dem an einen festen Träger gebundenen PstI-B-Fragment des SV40-Virus und dem mit einem Indikator markierten Pst I-A-Fragment des SV40-Virus besteht.
11. Reagenzkombination nach einem der Ansprüche 5 bis
10, dadurch gekennzeichnet, dass eines der Paare Nukleinsäure reagen zi en mit NukLeinsäure aus Bacillus amylolique fax Jens komplementär und damit hybridisierbar ist, wobei dieses Paar aus dem an einen festen Träger gebundenen EcoRl-BamHI-Fragment des cc-Amyla Segens aus Bacillus a m y IoIiquefa-eiens-Plasmid pKTHiO und dem mit einem Indikator markierten Clal-EcoRI-Fragment des α-Amy lasegens aus Ba-5 eil I us amy Io I iquefaciens-Plasmid ρ K T H1 0 besteht.
12. Reagenzkombination nach Anspruch 5, dadurch gekennzeichnet, dass eines der Paare NukIeinsäurereagenzien mit Nukleinsäure aus E. coli komplementär und damit hybridisierbar ist, wobei dieses Paar aus dem an einen festen Träger gebundenen Plasmid pKTH45 und dem mit einem
Indikator markierten Rekombinationsphagen mKTHi207 besteht.
DE198282305489T 1981-10-16 1982-10-15 Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer. Pending DE79139T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI813251A FI63596C (fi) 1981-10-16 1981-10-16 Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser

Publications (1)

Publication Number Publication Date
DE79139T1 true DE79139T1 (de) 1983-11-24

Family

ID=8514776

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198282305489T Pending DE79139T1 (de) 1981-10-16 1982-10-15 Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer.
DE8282305489T Expired DE3277917D1 (en) 1981-10-16 1982-10-15 A method and reagent combination for the identification of microorganisms and the use of sandwich hybridization of nucleic acids therefor

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8282305489T Expired DE3277917D1 (en) 1981-10-16 1982-10-15 A method and reagent combination for the identification of microorganisms and the use of sandwich hybridization of nucleic acids therefor

Country Status (13)

Country Link
US (2) US4486539A (de)
EP (2) EP0098267A1 (de)
JP (2) JPH0632637B1 (de)
AT (1) ATE31735T1 (de)
AU (1) AU548854B2 (de)
CA (1) CA1192120A (de)
DE (2) DE79139T1 (de)
DK (1) DK173744B1 (de)
FI (1) FI63596C (de)
HU (1) HU196242B (de)
RO (1) RO86356B (de)
SU (1) SU1386031A3 (de)
WO (1) WO1983001459A1 (de)

Families Citing this family (340)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US5723597A (en) * 1983-01-10 1998-03-03 Gen-Probe Incorporated Ribosomal nucleic acid probes for detecting organisms or groups of organisms
US5288611A (en) * 1983-01-10 1994-02-22 Gen-Probe Incorporated Method for detecting, identifying, and quantitating organisms and viruses
US5641632A (en) * 1983-01-10 1997-06-24 Gen-Probe Incorporated Method for preparing rRNA for hybridization with a probe
US4689295A (en) * 1983-01-20 1987-08-25 Integrated Genetics, Inc. Test for Salmonella
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
WO1984003285A1 (en) * 1983-02-22 1984-08-30 Molecular Biosystems Inc Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
US5593832A (en) * 1983-02-28 1997-01-14 Lifecodes Corporation Method for forensic analysis
IL71064A (en) * 1983-02-28 1989-10-31 Lifecodes Corp Paternity and forensic test involving analysis of dna polymorphic genetic regions
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
CA1222680A (en) * 1983-07-05 1987-06-09 Nanibhushan Dattagupta Testing dna samples for particular nucleotide sequences
US4737454A (en) * 1983-07-14 1988-04-12 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
US4959309A (en) * 1983-07-14 1990-09-25 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
CA1231303A (en) * 1983-08-05 1988-01-12 Robert J. Carrico Detection of bacteria by nucleic acid hybridization
US5539097A (en) * 1983-09-02 1996-07-23 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
JPS6091999A (ja) * 1983-10-25 1985-05-23 Fujirebio Inc ポリヌクレオチドの測定方法
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna
IL73577A (en) * 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
US4777129A (en) * 1983-12-12 1988-10-11 Molecular Diagnostics, Inc. Nucleic acid probe detectable by specific nucleic acid binding protein
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
US4849208A (en) * 1984-01-30 1989-07-18 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4843122A (en) * 1984-01-30 1989-06-27 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US5002885A (en) * 1984-01-30 1991-03-26 Enzo Biochem, Inc. Detectable molecules, method preparation and use
US4849505A (en) * 1984-01-30 1989-07-18 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4943523A (en) * 1984-01-30 1990-07-24 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
CA1223222A (en) * 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
EP0175776B1 (de) * 1984-04-05 1991-07-31 Howard Florey Institute Of Experimental Physiology And Medicine Hybridisierungs-histochemie
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US6221581B1 (en) * 1984-04-27 2001-04-24 Enzo Diagnostics, Inc. Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4670380A (en) * 1984-05-23 1987-06-02 Molecular Diagnostics, Inc. Assays utilizing labeled nucleic acid probes
US5132206A (en) * 1984-06-11 1992-07-21 Dreyer William J Fluorescent pigments for tagging biological molecules
US4749647A (en) * 1984-06-22 1988-06-07 Genetic Systems Corporation Polymerization-induced separation assay using recognition pairs
FR2567541B1 (fr) * 1984-07-13 1987-02-06 Pasteur Institut Sonde d'adn et procede pour la detection de " shigelles " et des souches entero-invasives de escherichia coli
US4755458A (en) * 1984-08-30 1988-07-05 Enzo Biochem, Inc. Composition and method for the detection of the presence of a polynucleotide sequence of interest
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes
US5955261A (en) * 1984-09-04 1999-09-21 Gen-Probe Incorporated Method for detecting the presence of group-specific viral mRNA in a sample
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
GB8432118D0 (en) * 1984-12-19 1985-01-30 Malcolm A D B Sandwich hybridisation technique
GB2179448B (en) * 1984-12-19 1989-06-07 Blair Malcolm A D Improved sandwich hybridisation technique for the detection of nucleotide sequences
US4670379A (en) * 1984-12-19 1987-06-02 E. I. Du Pont De Nemours And Company Polynucleotide hydridization assays employing catalyzed luminescence
FI72146C (fi) * 1985-01-02 1987-04-13 Orion Yhtymae Oy Foerfarande foer identifiering av nukleinsyror.
US4785086A (en) * 1985-01-17 1988-11-15 Integrated Genetics, Inc. Test for Campylobacter
US5851767A (en) * 1985-03-04 1998-12-22 The Regents Of The University Of California Detection of prokaryotic organism by DNA hybridization
US4792519A (en) * 1985-03-05 1988-12-20 International Technology Corporation Method for the monitoring and control of microbial populations
US5470974A (en) * 1985-03-15 1995-11-28 Neu-Gene Development Group Uncharged polynucleotide-binding polymers
US5217866A (en) * 1985-03-15 1993-06-08 Anti-Gene Development Group Polynucleotide assay reagent and method
US6197563B1 (en) 1985-03-28 2001-03-06 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences
US6040166A (en) 1985-03-28 2000-03-21 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences, including a probe
EP0200113A3 (de) * 1985-04-30 1987-03-18 Pandex Laboratories, Inc. Verfahren der Nukleinsäuren-Hybridisierungsprobe unter Zusatz eines lumineszierenden Labels
IL79112A (en) * 1985-06-13 1992-01-15 Abbott Lab Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay
US5273882A (en) * 1985-06-13 1993-12-28 Amgen Method and kit for performing nucleic acid hybridization assays
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US5641630A (en) * 1985-06-13 1997-06-24 Amgen Inc. Method and kit for performing nucleic acid hybridization assays
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
US4831125A (en) * 1985-07-01 1989-05-16 E. I. Du Pont De Nemours And Company DNA probe for corynebacterium kutscheri
US4824776A (en) * 1985-07-25 1989-04-25 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US5155216A (en) * 1985-08-15 1992-10-13 Amoco Corporation Nucleic acids labeled with a chemiluminescent acridine ester
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US5595908A (en) * 1985-09-26 1997-01-21 University Of Southern Mississipi Piezoelectric device for detection of polynucleotide hybridization
WO1987002708A1 (en) * 1985-10-24 1987-05-07 Siska Diagnostics, Inc. Lanthanide chelate-tagged nucleic acid probes
US4876186A (en) * 1985-11-05 1989-10-24 Battelle Development Corporation Detection and differentiation of coxiella burnetii in biological fluids
US5258283A (en) * 1985-11-05 1993-11-02 Battelle Memorial Institute Detection and differentiation of coxiella burnetii in biological fluids
EP0249628A4 (de) * 1985-12-03 1989-09-19 M Raafat El-Gewely Verfahren, satz und erzeugnis für krankheitsdiagnose.
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
JP3293820B2 (ja) * 1985-12-13 2002-06-17 エンゾ− バイオケム インコ−ポレイテツド 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物
FI76119C (fi) * 1986-02-27 1988-09-09 Orion Yhtymae Oy Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet.
US4968602A (en) * 1986-03-05 1990-11-06 Molecular Diagnostics, Inc. Solution-phase single hybridization assay for detecting polynucleotide sequences
US5242823A (en) * 1986-03-07 1993-09-07 International Genetic Engineering, Inc. Cloning of the 38kd Mycoplasma hyorhinis regression-associated antigen
US4925785A (en) * 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
DE3650043T2 (de) * 1986-06-25 1995-01-19 Univ California Nachweis von Mykoplasma durch Hybridisierung von DNS.
JP2534529B2 (ja) * 1986-07-24 1996-09-18 ブリティシュ・テレコミュニケ−ションズ・パブリック・リミテッド・カンパニ 放射発生器
JP2641880B2 (ja) * 1986-08-11 1997-08-20 シスカ・ダイアグノスティックス・インコーポレーテッド 核酸プローブアッセイ法および組成物
US5849478A (en) * 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
US4917999A (en) * 1986-09-02 1990-04-17 Miles Inc. Oligonucleotide probes for detection of α-amylase genes
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US7090972B1 (en) 1986-11-24 2006-08-15 Gen-Probe Incorporated Methods for determining the presence of non-viral organisms in a sample
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US6150517A (en) 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US7087742B1 (en) 1986-11-24 2006-08-08 Gen-Probe Incorporated Oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US5994059A (en) * 1986-11-24 1999-11-30 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Streptomyces enterococci
US5145776A (en) * 1987-01-14 1992-09-08 President & Fellows Of Harvard College Method of using T7 DNA polymerase to mutagenize and fill-in DNA
US4946786A (en) * 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
US5266466A (en) * 1987-01-14 1993-11-30 President And Fellows Of Harvard College Method of using T7 DNA polymerase to label the 3' end of a DNA molecule
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US4994372A (en) * 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
CA1317860C (en) * 1987-04-01 1993-05-18 Daniel Louis Kacian Techniques for preparing specimens for bacterial assays
US5225324A (en) * 1987-04-24 1993-07-06 Bioscience International, Inc. Diagnostics for mycobacteria in public health, medical, and veterinary practice
GB8709803D0 (en) * 1987-04-24 1987-05-28 Mcfadden J J Treatment of crohn's disease &c
US4994368A (en) 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
KR960002234B1 (ko) * 1987-07-31 1996-02-13 젠-프로우브 인코오퍼레이티드 바람직하지 못한 교차반응을 제거하기 위한 올리고뉴클레오티드를 사용하는 폴리뉴클레오티드에 대한 측정방법
US5089386A (en) * 1987-09-11 1992-02-18 Gene-Trak Systems Test for listeria
US5656731A (en) * 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
WO1989004375A1 (en) * 1987-10-23 1989-05-18 Siska Diagnostics, Inc. Lanthanide chelate-tagged nucleic acid probes
US6013531A (en) * 1987-10-26 2000-01-11 Dade International Inc. Method to use fluorescent magnetic polymer particles as markers in an immunoassay
CA1340506C (en) * 1987-11-24 1999-04-20 Nicholas H. Carbonetti Production of gonorrheal pi proteins and vaccines
US6348332B1 (en) 1987-11-24 2002-02-19 The University Of North Carolina At Chapel Hill DNA molecule encoding gonorrhoeal hybrid PIA/PIB protein
EP0389564B1 (de) * 1987-12-01 1996-01-24 Amoco Corporation Nachweis von salmonella
US5354657A (en) * 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US20050032048A1 (en) * 1988-05-03 2005-02-10 Oxford Gene Technology Limited Analyzing polynucleotide sequences
US5700637A (en) * 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US6054270A (en) * 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US7811751B2 (en) 1988-05-03 2010-10-12 Oxford Gene Technology Limited Analysing polynucleotide sequences
US5024933A (en) * 1988-05-10 1991-06-18 Enzo Biochem, Inc. Method and kit for sample adherence to test substrate
DE68919706T2 (de) * 1988-05-10 1995-04-13 Du Pont Verfahren zum schnellen nachweis von nukleinsäuren.
US5294533A (en) * 1988-07-05 1994-03-15 Baylor College Of Medicine Antisense oligonucleotide antibiotics complementary to the macromolecular synthesis operon, methods of treating bacterial infections and methods for identification of bacteria
US5047523A (en) * 1988-08-02 1991-09-10 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of neisseria gonorrhoea
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
WO1990006372A1 (en) * 1988-12-01 1990-06-14 Microprobe Corporation Substrates for peroxidase assaying
US7172863B1 (en) 1988-12-09 2007-02-06 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Neisseria gonorrhoeae
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
CA2005927A1 (en) * 1988-12-21 1990-06-21 Chander Bahl Method of preparing nucleotide probes using a bridging complement
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5514550A (en) * 1989-02-03 1996-05-07 Johnson & Johnson Clinical Diagnostics, Inc. Nucleic acid test article and its use to detect a predetermined nucleic acid
CA2009708A1 (en) * 1989-02-13 1990-08-13 Jane D. Madonna Nucleic acid probe for the detection of salmonella human pathogens
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US5049489A (en) * 1989-04-17 1991-09-17 The Standard Oil Company 16S rRNA oligonucleotide probes for the identification of sulfate-reducing bacteria
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6416952B1 (en) 1989-06-07 2002-07-09 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US6955915B2 (en) * 1989-06-07 2005-10-18 Affymetrix, Inc. Apparatus comprising polymers
US6919211B1 (en) * 1989-06-07 2005-07-19 Affymetrix, Inc. Polypeptide arrays
US5925525A (en) * 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
EP0405592A3 (en) * 1989-06-30 1991-07-17 Sanyo Chemical Industries Ltd. A method for detection of nucleic acid and reagents for their detection
US5106730A (en) * 1989-07-24 1992-04-21 Microprobe Corporation Lactam-containing compositions and methods useful for the hybridization of nucleic acids
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
CA2028012A1 (en) * 1989-10-23 1991-04-24 Randall Dimond Hybridization assay for campylobacter rrna
GB8924989D0 (en) * 1989-11-06 1989-12-28 Scotgen Ltd Method and device for the detection of antibiotic resistance
WO1991009141A1 (en) * 1989-12-14 1991-06-27 Baxter Diagnostics Inc. Magnetically responsive fluorescent polymer particles and application thereof
CA2032203C (en) * 1989-12-29 2009-05-19 Christine L. Brakel Amplification capture assay
CA2075186A1 (en) * 1990-02-02 1991-08-03 Rudi Rossau Hybridization probes for the detection of branhamella catarrhalis strains
US5721097A (en) * 1990-02-02 1998-02-24 N. V. Innogenetics S.A. Hybridization probes for the detection of branhamella catarrhalis strains
US6013431A (en) * 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
AU7429591A (en) * 1990-04-18 1991-10-24 Gene-Trak Systems Nucleic acid probes for the detection of giardia lamblia
RU2107730C1 (ru) * 1990-05-04 1998-03-27 Чирон Корпорейшн Молекулярный зонд
CA2116215C (en) * 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
US5232830A (en) * 1990-05-11 1993-08-03 Microprobe Corporation Intrinsic fluorescent quenching methods
US5695926A (en) * 1990-06-11 1997-12-09 Bio Merieux Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support
IE912586A1 (en) * 1990-07-24 1992-01-29 Uab Research Foundation Bovine respiratory syncytial virus vaccines
US6730305B1 (en) 2000-05-08 2004-05-04 The Uab Research Foundation Nucleotide sequences encoding bovine respiratory syncytial virus immunogenic proteins
IL99025A0 (en) * 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
AU9115891A (en) * 1990-11-14 1992-06-11 Siska Diagnostics, Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
DE69132843T2 (de) * 1990-12-06 2002-09-12 Affymetrix Inc N D Ges D Staat Identifizierung von Nukleinsäuren in Proben
USH1398H (en) * 1990-12-28 1995-01-03 Campbell; James R. DNA-based fluourescent sensor
EP0497464A1 (de) * 1991-01-31 1992-08-05 Amoco Corporation Schnellnachweis von Mikroben durch in situ-Hybridisation in wässeriger Suspension
EP0525181B1 (de) * 1991-02-14 1997-08-06 Dade MicroScan Inc. Neue oligonukleotide konjugiert zu lanthanide-chelaten
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
EP0533952A4 (en) * 1991-04-15 1993-07-07 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
US5556748A (en) * 1991-07-30 1996-09-17 Xenopore Corporation Methods of sandwich hybridization for the quantitative analysis of oligonucleotides
US5612199A (en) * 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
CA2080305A1 (en) 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
US6569382B1 (en) * 1991-11-07 2003-05-27 Nanogen, Inc. Methods apparatus for the electronic, homogeneous assembly and fabrication of devices
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6652808B1 (en) 1991-11-07 2003-11-25 Nanotronics, Inc. Methods for the electronic assembly and fabrication of devices
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
ES2049618B1 (es) * 1991-11-13 1994-11-01 Consejo Superior Investigacion Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi.
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
US5543292A (en) * 1992-06-16 1996-08-06 Hitachi, Ltd. Process for the measurement of nucleic acids
DE69310179T2 (de) * 1992-07-31 1997-07-31 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
US7713528B1 (en) 1993-02-18 2010-05-11 Enzo Therapeutics, Inc. Method for in vivo delivery of active compounds using reagent conjugate
US6294323B1 (en) 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids
JPH08510649A (ja) * 1993-05-28 1996-11-12 マウント シナイ スクール オブ メディシン オブ ザ シティー ユニバーシティー オブ ニューヨーク Ｂｃ２００ｒｎａ、それに対するプローブ及びその使用
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren
US5854084A (en) 1996-07-12 1998-12-29 Biotraces, Inc. Enhanced chromatography using multiphoton detection
US5532122A (en) * 1993-10-12 1996-07-02 Biotraces, Inc. Quantitation of gamma and x-ray emitting isotopes
US7314708B1 (en) 1998-08-04 2008-01-01 Nanogen, Inc. Method and apparatus for electronic synthesis of molecular structures
US7101661B1 (en) 1993-11-01 2006-09-05 Nanogen, Inc. Apparatus for active programmable matrix devices
US6068818A (en) 1993-11-01 2000-05-30 Nanogen, Inc. Multicomponent devices for molecular biological analysis and diagnostics
US6726880B1 (en) 1993-11-01 2004-04-27 Nanogen, Inc. Electronic device for performing active biological operations and method of using same
US6254827B1 (en) 1993-11-01 2001-07-03 Nanogen, Inc. Methods for fabricating multi-component devices for molecular biological analysis and diagnostics
US7172864B1 (en) 1993-11-01 2007-02-06 Nanogen Methods for electronically-controlled enzymatic reactions
US6638482B1 (en) 1993-11-01 2003-10-28 Nanogen, Inc. Reconfigurable detection and analysis apparatus and method
US6319472B1 (en) 1993-11-01 2001-11-20 Nanogen, Inc. System including functionally separated regions in electrophoretic system
US6287517B1 (en) 1993-11-01 2001-09-11 Nanogen, Inc. Laminated assembly for active bioelectronic devices
US6309602B1 (en) 1993-11-01 2001-10-30 Nanogen, Inc. Stacked, reconfigurable system for electrophoretic transport of charged materials
US20040077074A1 (en) * 1993-11-01 2004-04-22 Nanogen, Inc. Multi-chambered analysis device
US6375899B1 (en) 1993-11-01 2002-04-23 Nanogen, Inc. Electrophoretic buss for transport of charged materials in a multi-chamber system
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6315953B1 (en) 1993-11-01 2001-11-13 Nanogen, Inc. Devices for molecular biological analysis and diagnostics including waveguides
KR100230909B1 (ko) * 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법
EP0754241B1 (de) * 1994-04-04 1998-12-02 Ciba Corning Diagnostics Corp. Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen
US7378236B1 (en) 1994-06-17 2008-05-27 The Board Of Trustees Of The Leland Stanford Junior University Method for analyzing gene expression patterns
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US7323298B1 (en) * 1994-06-17 2008-01-29 The Board Of Trustees Of The Leland Stanford Junior University Microarray for determining the relative abundances of polynuceotide sequences
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US5545527A (en) * 1994-07-08 1996-08-13 Visible Genetics Inc. Method for testing for mutations in DNA from a patient sample
US8236493B2 (en) 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
CA2163393C (en) * 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5783387A (en) * 1995-02-06 1998-07-21 The Regents Of The University Of California Method for identifying and quantifying nucleic acid sequence aberrations
US5731153A (en) * 1996-08-26 1998-03-24 The Regents Of The University Of California Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions
US5610023A (en) * 1995-03-31 1997-03-11 Lee Laboratories, Inc. Method of purification of clostridium difficile toxin A and production of mono-specific antibodies
US5783453A (en) * 1995-06-29 1998-07-21 Chiron Diagnostics Corporation Non-separation specific binding chemiluminescent assay
US5616465A (en) * 1995-08-09 1997-04-01 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using competitive hybridization probes
US6027879A (en) * 1995-08-09 2000-02-22 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US20040086917A1 (en) * 1995-09-27 2004-05-06 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
EP0880598A4 (de) 1996-01-23 2005-02-23 Affymetrix Inc Verfahren zur analyse von nukleinsäure
AU2320597A (en) 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US5814490A (en) * 1996-06-11 1998-09-29 Becton, Dickinson And Company Amplification and detection of chlamydia trachomatis nucleic acids
US5910410A (en) * 1996-09-06 1999-06-08 Hewlett-Packard Company Dual tag binding assay
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US6706473B1 (en) 1996-12-06 2004-03-16 Nanogen, Inc. Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides
WO1998029736A1 (en) * 1996-12-31 1998-07-09 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
WO1998050583A1 (en) 1997-05-02 1998-11-12 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
DE19741715A1 (de) * 1997-09-22 1999-03-25 Hoechst Ag Pentopyranosyl-Nucleosid, seine Herstellung und Verwendung
ES2246546T3 (es) * 1997-12-12 2006-02-16 Digene Corporation Valoracion de enfermedades relacionadas con el virus de papiloma humano.
US20030096232A1 (en) * 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US20030039967A1 (en) * 1997-12-19 2003-02-27 Kris Richard M. High throughput assay system using mass spectrometry
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US6232066B1 (en) 1997-12-19 2001-05-15 Neogen, Inc. High throughput assay system
US6458533B1 (en) 1997-12-19 2002-10-01 High Throughput Genomics, Inc. High throughput assay system for monitoring ESTs
DE1075328T1 (de) 1998-05-01 2001-10-11 Ammann Kelly G Automatische diagnostische analysevorrichtung und -verfahren
DE19824900B4 (de) 1998-06-04 2006-05-04 Roche Diagnostics Gmbh DNA-Nachweis über einen Strangreassoziationskomplex
CA2364305A1 (en) 1999-03-31 2000-10-05 The University Of North Carolina At Chapel Hill Isolated dna encoding cullin regulators roc1 and roc2, isolated proteins encoded by the same, and methods utilizing the same
US6235479B1 (en) 1999-04-13 2001-05-22 Bio Merieux, Inc. Methods and devices for performing analysis of a nucleic acid sample
US7101989B1 (en) 1999-07-09 2006-09-05 University Of North Carolina At Chapel Hill DsrA protein and polynucleotides encoding the same
FR2803913B1 (fr) * 2000-01-13 2002-08-16 Pasteur Sanofi Diagnostics Procede d'immobilisation de reactif(s) affin(s) sur phase solide hydrophobe
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
JP2003521252A (ja) * 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
AU2001247328C1 (en) * 2000-03-08 2006-10-26 Datascope Investment Corp. Methods for assay and detection on a microarray
AU3460600A (en) * 2000-03-16 2001-09-24 Bionex, Inc. Method for detecting mutation of nucleic acid
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US20040185464A1 (en) * 2000-09-15 2004-09-23 Kris Richard M. High throughput assay system
US20020169562A1 (en) * 2001-01-29 2002-11-14 Gregory Stephanopoulos Defining biological states and related genes, proteins and patterns
EP1481076A4 (de) 2001-07-12 2005-05-11 Illumina Inc Multiplex-nukleinsäurereaktionen
US6893822B2 (en) 2001-07-19 2005-05-17 Nanogen Recognomics Gmbh Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
US20050147984A1 (en) * 2001-08-31 2005-07-07 Clondiag Chip Technologies Gmbh Interaction detection on several probe arrays
US6696254B2 (en) * 2001-11-21 2004-02-24 Kimberly-Clark Worldwide, Inc. Detection and identification of enteric bacteria
US7041811B2 (en) * 2001-12-19 2006-05-09 Dr. Chip Biotechnology, Inc. Method for detecting Escherichia coli
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
US7579147B2 (en) 2002-05-07 2009-08-25 Wake Forest University Health Sciences Mutations in the macrophage scavenger receptor 1 gene alter risk of prostate cancer, asthma, and cardiovascular disease
US20040029260A1 (en) * 2002-05-17 2004-02-12 Hansen Timothy R. Automated system for isolating, amplifying and detecting a target nucleic acid sequence
US20030219755A1 (en) * 2002-05-24 2003-11-27 Nanibhushan Dattagupta Compositions and methods for performing hybridization assays using target enhanced signal amplification (TESA)
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US20050037369A1 (en) * 2002-10-31 2005-02-17 Neote Kuldeep Singh Panels of molecular targets differentially expressed during CD8+ T cell priming, and methods for therapy and diagnosis utilizing the same
EP1567674A4 (de) * 2002-11-22 2007-09-26 Univ Johns Hopkins Ziel für die therapie einer kognitivenbeeinträchtigung
AU2003296014B2 (en) * 2002-11-26 2010-06-03 University Of Maryland Biotechnology Institute High-sensitivity assays for pathogen detection using metal-enhanced fluorescence
US20040235019A1 (en) * 2003-03-06 2004-11-25 Agouron Pharmaceuticals, Inc. Diagnostics and therapeutics for the gene expression signature of PPAR-gamma receptor ligand
EP1633893B1 (de) 2003-05-19 2012-01-25 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zur bestimmung des vorhandenseins von trichomonas vaginalis in einer testprobe
CA2545917C (en) 2003-11-14 2014-07-29 Duke University Methods of detecting charcot-marie tooth disease type 2a
GB0411081D0 (en) * 2004-05-18 2004-06-23 Glaxo Group Ltd Novel apparatus
DE602005020943D1 (de) 2004-07-01 2010-06-10 Gen Probe Inc Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
US7662562B2 (en) * 2004-08-10 2010-02-16 Becton, Dickinson And Company Method for rapid identification of microorganisms
US7828954B2 (en) * 2004-09-21 2010-11-09 Gamida For Life B.V. Electrode based patterning of thin film self-assembled nanoparticles
US7314542B2 (en) * 2004-09-23 2008-01-01 Nanogen, Inc. Methods and materials for optimization of electronic transportation and hybridization reactions
CA2842232C (en) 2005-03-10 2015-01-27 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
EP2348320B1 (de) 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
WO2006110314A2 (en) 2005-03-25 2006-10-19 Ambion, Inc. Methods and compositions for depleting abundant rna transcripts
JP5187847B2 (ja) 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド 核酸標的の捕獲方法
EP1971690B1 (de) 2005-12-28 2018-10-17 Translational Therapeutics, Inc. Auf translationaler fehlfunktion beruhende therapeutika
WO2008060369A2 (en) 2006-09-29 2008-05-22 Translational Therapeutics, Inc. Eif4e regulon-based diagnostics
US20080118914A1 (en) * 2006-01-04 2008-05-22 Charlotte Dawn Blowe Follistatin gene as a genetic marker for first parity litter size in pigs
US20070186298A1 (en) * 2006-01-04 2007-08-09 Blowe Charlotte D Follistatin gene as a genetic marker for reproductive and performance traits in pigs
AU2007267517B2 (en) 2006-05-24 2012-12-20 Gen-Probe Incorporated Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe
PL2017356T3 (pl) 2006-06-06 2012-03-30 Gen Probe Inc Znakowane oligonukleotydy i ich zastosowanie w sposobach amplifikacji kwasu nukleinowego
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
EP1978111B1 (de) 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
WO2009085221A2 (en) 2007-12-21 2009-07-09 Biomerieux Sa Detection of methicillin-resistant staphylococcus aureus
WO2009126336A1 (en) * 2008-04-11 2009-10-15 Becton, Dickinson And Company Methods of controlling the sensitivity and dynamic range of a homogeneous assay
EP2806037B1 (de) 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
US8703421B2 (en) 2008-05-30 2014-04-22 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
CN102177436B (zh) 2008-08-12 2015-05-13 金帆德尔制药股份有限公司 鉴定疾病风险因子的方法
US8288520B2 (en) * 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
EP2367958B1 (de) * 2008-11-25 2017-08-23 Gen-Probe Incorporated ZUSAMMENSETZUNGEN UND VERFAHREN ZUR ERKENNUNG VON sRNA UND IHRE VERWENDUNG
AU2009335053B2 (en) 2008-12-30 2013-08-01 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Listeria
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
US9797000B2 (en) * 2009-05-01 2017-10-24 Qiagen Gaithersburg Inc. Non-target amplification method for detection of RNA splice-forms in a sample
CN105618264B (zh) 2009-05-15 2021-04-06 简·探针公司 用于在执行磁性分离工序的仪器中实现磁体的自动移动的方法和设备
CA2770888C (en) 2009-08-31 2020-04-14 Gen-Probe Incorporated Dengue virus assay
EP2478087B1 (de) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Zusammensetzungen und verfahren zur rückgewinnung von nukleinsäuren oder proteinen aus in zytologischen medien fixierten gewebeproben
AU2010339861A1 (en) 2009-12-21 2012-10-25 Becton, Dickinson And Company Methods for identifying drug resistant Mycobacterium
US9404160B2 (en) 2009-12-22 2016-08-02 Becton, Dickinson And Company Methods for the detection of microorganisms
WO2011091330A1 (en) 2010-01-22 2011-07-28 Gen-Probe Incorporated Probes for detecting the presence of trichomonas vaginalis in a sample
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
CN102822189B (zh) 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
KR20130080007A (ko) 2010-05-11 2013-07-11 다우 아그로사이언시즈 엘엘씨 Ｓｐｎｋ 균주
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
AU2011258501B2 (en) 2010-05-25 2016-07-07 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
CN103540518B (zh) 2010-07-23 2015-07-08 贝克曼考尔特公司 化验盒
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
DK2520653T3 (en) 2011-05-03 2017-07-03 Dow Agrosciences Llc INTEGRATION OF GENES IN THE CHROMOSOME OF SACCHAROPOLYSPORA SPINOSA
EP3620533B1 (de) 2011-09-06 2023-01-18 Gen-Probe Incorporated Geschlossene nukleinsäurestrukturen
DE102011114984B3 (de) * 2011-09-28 2012-11-22 Universität Rostock Sequenzspezifische Analyse von Nukleinsäuren
US8973736B2 (en) 2011-11-07 2015-03-10 Beckman Coulter, Inc. Magnetic damping for specimen transport system
WO2013070740A1 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Aliquotter system and workflow
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
CN104053997B (zh) 2011-11-07 2016-12-21 贝克曼考尔特公司 用于处理样本的系统和方法
BR112014011046A2 (pt) 2011-11-07 2017-06-13 Beckman Coulter, Inc. fluxo de trabalho e sistema de centrífuga
WO2013070754A1 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Robotic arm
EP3450572A1 (de) 2011-12-23 2019-03-06 bioMérieux Nachweis von meca-varianten-stämmen von methicillin-resistentem staphylococcus aureus
US9631195B2 (en) 2011-12-28 2017-04-25 Dow Agrosciences Llc Identification and characterization of the spinactin biosysnthesis gene cluster from spinosyn producing saccharopolyspora spinosa
CA2865541C (en) * 2012-02-27 2019-11-05 Toray Industries, Inc. Nucleic acid detection method
WO2013181553A2 (en) 2012-06-01 2013-12-05 Akron Biotech Detection of mycoplasma in cell cultures and cell culture derived biologicals
WO2014006025A2 (en) 2012-07-02 2014-01-09 INSERM (Institut National de la Santé et de la Recherche Médicale) Marker of pathogenicity in salmonella
BR112015004537A2 (pt) * 2012-08-31 2017-07-04 Toray Industries método para detecção de um ácido nucleico alvo por hibridização sanduíche
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
EP3841223A1 (de) 2018-08-08 2021-06-30 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis von mycoplasma genitalium
EP3914732A1 (de) 2019-01-25 2021-12-01 Gen-Probe Incorporated Nachweis von arzneimittelresistentem mycoplasma genitalium
AU2020335996A1 (en) 2019-08-23 2022-03-31 Gen-Probe Incorporated Compositions, methods and kits for detecting Treponema pallidum
AU2021308095A1 (en) 2020-07-17 2023-03-09 Gen-Probe Incorporated Detection of macrolide-resistant mycoplasma genitalium

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3896217A (en) * 1973-03-19 1975-07-22 Summa Corp Method and apparatus for radioimmunoassay with regeneration of immunoadsorbent
US3930956A (en) * 1973-10-29 1976-01-06 The Regents Of The University Of Michigan Method for the genetic detection of microorganisms
SU649751A1 (ru) * 1977-06-14 1979-02-28 Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им.М.В.Ломоносова Способ идентификации микроорганизмов
US4139346A (en) * 1977-11-28 1979-02-13 Enzo Bio Chem Incorporated Nucleic acid and protein binding paper
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
FR2444713A1 (fr) * 1978-12-18 1980-07-18 Pasteur Institut Procede de production d'un adn comprenant le genome du virus de l'hepatite b et vecteur le comportant
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4359535A (en) * 1979-10-01 1982-11-16 George Pieczenik Autonomously replicating DNA containing inserted DNA sequences
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4446237A (en) * 1981-03-27 1984-05-01 Life Technologies, Inc. Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid
EP0062286A1 (de) * 1981-03-31 1982-10-13 Albert Einstein College Of Medicine Of Yeshiva University Diagnostischer Nachweis von Hepatitis-B-Virus
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
JPS5840099A (ja) * 1981-07-17 1983-03-08 アモコ・コ−ポレ−ション 光−放射ポリヌクレオチドの交雑診断方法
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser

Also Published As

Publication number Publication date
WO1983001459A1 (en) 1983-04-28
EP0079139B1 (de) 1988-01-07
AU548854B2 (en) 1986-01-02
RO86356B (ro) 1985-04-01
FI63596C (fi) 1983-07-11
JPH0632637B1 (de) 1994-05-02
DK275183D0 (da) 1983-06-15
DK173744B1 (da) 2001-09-03
EP0098267A1 (de) 1984-01-18
DE3277917D1 (en) 1988-02-11
DK275183A (da) 1983-06-15
SU1386031A3 (ru) 1988-03-30
US4563419A (en) 1986-01-07
EP0079139A1 (de) 1983-05-18
AU8957582A (en) 1983-05-05
RO86356A (ro) 1985-03-15
ATE31735T1 (de) 1988-01-15
CA1192120A (en) 1985-08-20
FI63596B (fi) 1983-03-31
HU196242B (en) 1988-10-28
US4486539A (en) 1984-12-04
JPS58501703A (ja) 1983-10-13

Similar Documents

Publication Publication Date Title
DE79139T1 (de) Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer.
Nielsen et al. Evaluation of phenotypic and genotypic methods for subtyping Campylobacter jejuni isolates from humans, poultry, and cattle
DE69937447T2 (de) Verfahren zur erkennung von nukleinsäuren
DE69434255T2 (de) Nukleinsäuresonde zum nachweis von lactobacillus
Blackall, JK Miflin et al. Identification and typing of Pasteurella multocida: a review
Occhialini et al. Distribution of open reading frames of plasticity region of strain J99 in Helicobacter pylori strains isolated from gastric carcinoma and gastritis patients in Costa Rica
EP0173339A3 (en) Composition and method for the dectection of the presence of a polynucleotide sequence of interest
DE07025195T1 (de) Nukleotidsequenzen des Genoms von Retroviren des Typs HIV-1, HIV-2 und SIV und deren Anwendungen, insbesondere zur Amplifikation von Genomen dieser Retroviren und für die In-Vitro-Diagnostik von durch diese Viren ausgelösten Infektionen
DE135159T1 (de) Monoklonale antikoerper spezifisch fuer die doppelstraengige struktur von nativem dns und seine verwendung in diagnostischen methoden.
Chachaty et al. Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typing Clostridium difficile strains
DE19515891C2 (de) Gattungs- und speziesspezifische Identifizierung von Legionellen
Stackebrandt et al. DNA-DNA homology studies among strains of Arthrobacter and Brevibacterium
DE69634790T2 (de) Oligonukleotide für den Nachweis von Salmonella
US5656740A (en) Nucleic acid fragments useful in the detection of Salmonella
León et al. A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum
EP0408077B1 (de) Neisseria gonorrhoeae-spezifische Oligonuleotid-Sonden
Schares et al. Sleeping sickness in Zaire: a nested polymerase chain reaction improves the identification of Trypanosoma (Trypanozoon) brucei gambiense by specific kinetoplast DNA probes
DE60114816T2 (de) Umgekehrter nachweis zur identifizierung und/oder quantifizierung von nukleotid-zielsequenzen mittels biochips
DE60129896T2 (de) Amplifizierung und Detektion von Mycoplasma pneumoniae
Samore et al. Comparison of arbitrarily-primed polymerase chain reaction, restriction enzyme analysis and pulsed-field gel electrophoresis for typing Clostridium difficile
WO2001068900A2 (de) Verfahren zum spezifischen nachweis von mikroorganismen durch polymerase-kettenreaktion
DE3938853A1 (de) Verfahren zum nachweis abweichender nukleotidsequenzen, hybridisierungssonden fuer dieses verfahren sowie derartige sonden enthaltender kit
WO1995031569A1 (en) Detection and differentiation of mycobacterium tuberculosis complex bacteria by direct variant repeat oligotyping
Hartmann et al. Bradyrhizobium japonicum strain identification by RFLP analysis using the repeated sequence RSα
DE3420925A1 (de) Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz