DE69836012D1 - Zwei-schritt hybridisierung und einfang von einem polynukleotid - Google Patents

Zwei-schritt hybridisierung und einfang von einem polynukleotid

Info

Publication number
DE69836012D1
DE69836012D1 DE69836012T DE69836012T DE69836012D1 DE 69836012 D1 DE69836012 D1 DE 69836012D1 DE 69836012 T DE69836012 T DE 69836012T DE 69836012 T DE69836012 T DE 69836012T DE 69836012 D1 DE69836012 D1 DE 69836012D1
Authority
DE
Germany
Prior art keywords
target polynucleotide
hybridization
hybridization conditions
polynucleotide
capture probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69836012T
Other languages
English (en)
Other versions
DE69836012T2 (de
Inventor
G Weisburg
H Shaw
M Becker
Mehrdad Majlessi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of DE69836012D1 publication Critical patent/DE69836012D1/de
Publication of DE69836012T2 publication Critical patent/DE69836012T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
DE69836012T 1997-05-02 1998-05-01 Zwei-schritt hybridisierung und einfang von einem polynukleotid Expired - Lifetime DE69836012T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4543097P 1997-05-02 1997-05-02
US45430P 1997-05-02
PCT/US1998/008853 WO1998050583A1 (en) 1997-05-02 1998-05-01 Two-step hybridization and capture of a polynucleotide

Publications (2)

Publication Number Publication Date
DE69836012D1 true DE69836012D1 (de) 2006-11-09
DE69836012T2 DE69836012T2 (de) 2007-04-05

Family

ID=21937832

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69836012T Expired - Lifetime DE69836012T2 (de) 1997-05-02 1998-05-01 Zwei-schritt hybridisierung und einfang von einem polynukleotid

Country Status (11)

Country Link
US (3) US6110678A (de)
EP (1) EP0975807B1 (de)
JP (1) JP4222635B2 (de)
KR (1) KR20010012175A (de)
AT (1) ATE340868T1 (de)
AU (1) AU738708B2 (de)
CA (1) CA2287570C (de)
DE (1) DE69836012T2 (de)
DK (1) DK0975807T3 (de)
ES (1) ES2271994T3 (de)
WO (1) WO1998050583A1 (de)

Families Citing this family (203)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
ES2324503T3 (es) 1997-04-10 2009-08-07 Stichting Katholieke Universiteit University Medical Centre Nijmegen Pca3, genes pca3 y metodos de uso.
EP0975807B1 (de) * 1997-05-02 2006-09-27 Gen-Probe Incorporated Zwei-schritt hybridisierung und einfang von einem polynukleotid
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6849400B1 (en) 1997-07-23 2005-02-01 Gen-Probe Incorporated Methods for detecting and measuring spliced nucleic acids
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
AU4070499A (en) 1998-04-30 1999-11-16 Cornell Research Foundation Inc. Adenoviral vectors with tandem fiber proteins
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
EP1614475B1 (de) 1998-05-01 2007-05-30 Gen-Probe Incorporated Rührvorrichtung für den fluiden Inhalt eines Behälters
EP2045334A1 (de) 1998-06-24 2009-04-08 Illumina, Inc. Dekodierung von Arraysensoren mit Mikrosphären
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
EP2360270B1 (de) 1999-05-20 2016-11-09 Illumina, Inc. Kombinatorische Entschlüsselung von Zufallsnukleinsäurearrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
EP1422298B1 (de) * 1999-07-09 2010-01-27 Gen-Probe Incorporated HIV-1 Detektion mittels Nukleinsäureamplifizierung
EP1218545B1 (de) 1999-08-18 2012-01-25 Illumina, Inc. Methoden zur erzeugung von oligonukleotidlösungen
AU7636400A (en) * 1999-09-29 2001-04-30 Diagnocure Inc. Pca3 messenger rna species in benign and malignant prostate tissues
US6428957B1 (en) * 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
ES2253279T3 (es) 1999-12-15 2006-06-01 Gen-Probe Incorporated Metodos y composiciones para la deteccion de especies del complejo de mycobacterium avium.
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
ATE495268T1 (de) * 1999-12-17 2011-01-15 Gen Probe Inc Nukleinsäureamplifizierung und detektion von mykobacterium-spezies
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US6913884B2 (en) * 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
EP1259643B1 (de) * 2000-02-07 2008-10-15 Illumina, Inc. Nukleinsäure-nachweisverfahren mit universellem priming
AU2001238067B2 (en) 2000-02-07 2007-01-25 Illumina, Inc. Nucleic acid detection methods using universal priming
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US20020039728A1 (en) * 2000-02-10 2002-04-04 Robert Kain Alternative substrates and formats for bead-based array of arrays
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
WO2001061043A2 (en) 2000-02-16 2001-08-23 Illumina, Inc. Parallel genotyping of multiple patient samples
EP1263773A4 (de) 2000-03-14 2005-08-31 Active Motif Analoge von oligonukleotiden, methoden der synthese und methoden der verwendung
US6962906B2 (en) * 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US20040014644A1 (en) * 2000-03-14 2004-01-22 Vladimir Efimov Oligonucleotide analogues and methods of use for modulating gene expression
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
DE60040874D1 (de) 2000-09-01 2009-01-02 Gen Probe Inc Amplifizierung von hiv-1 sequenzen zur detektion ven einhergehen
ES2300375T3 (es) 2000-10-23 2008-06-16 Gen-Probe Incorporated Composiciones y metodos para la deteccion del virus de la inmunodeficiencia humana 2 (hiv-2).
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
DE10126630A1 (de) * 2001-05-31 2003-01-09 Peter Und Traudl Engelhorn Sti Verfahren zur Zellsortierung
JP5100956B2 (ja) 2001-08-31 2012-12-19 ジェン−プローブ・インコーポレーテッド ヒトパルボウイルスb19核酸の検出のためのアッセイ
US20030143527A1 (en) * 2001-10-09 2003-07-31 Venkatakrishna Shyamala Identification of oligonucleotides for the capture, detection and quantitation of hepatitis B viral DNA
WO2003045310A2 (en) * 2001-11-21 2003-06-05 Applera Corporation Digital assay
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
US7499806B2 (en) 2002-02-14 2009-03-03 Illumina, Inc. Image processing in microsphere arrays
AU2003213107A1 (en) 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
DE10211321A1 (de) * 2002-03-14 2003-09-25 Gnothis Holding Sa Ecublens Verwendung von Abfangsonden beim Nachweis von Nukleinsäuren
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
AU2003242605A1 (en) * 2002-06-03 2003-12-19 Pamgene B.V. Novel high density arrays and methods for analyte analysis
MXPA04012501A (es) * 2002-06-12 2005-02-17 Chiron Corp Identificacion de oligonucleotidos para la captura, deteccion y cuatificacion de acido nucleico viral de la hepatitis a.
ATE519861T1 (de) 2002-06-14 2011-08-15 Gen Probe Inc Zusammensetzungen zum nachweis von hepatitis-b- virus
CA2501946C (en) 2002-10-16 2014-12-23 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
GB2395557A (en) * 2002-11-22 2004-05-26 Dynal Biotech Ltd Nucleic acid probes
CA2513780C (en) * 2003-02-07 2014-12-30 Diagnocure Inc. Method to detect prostate cancer from a urine sample
US7381811B2 (en) 2003-05-19 2008-06-03 Gen-Probe Incorporated Compositions and methods for detecting Trichomonas vaginalis in a sample
CA2432365A1 (en) * 2003-06-30 2004-12-30 Jack A. Schalken Specific method of prostate cancer detection based on pca3, and kits therefore
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
AU2004303886B2 (en) 2003-12-19 2009-09-03 Gen-Probe Incorporated Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2
US20060266136A1 (en) * 2004-06-08 2006-11-30 Gnothis Holding Ag Bi-fluorophore marked probes for detecting nucleic acids
ATE466107T1 (de) 2004-07-01 2010-05-15 Gen Probe Inc Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
CA2838428C (en) * 2004-07-13 2015-09-29 Gen-Probe Incorporated Compositions and methods for detection of hepatitis a virus nucleic acid
US7662562B2 (en) * 2004-08-10 2010-02-16 Becton, Dickinson And Company Method for rapid identification of microorganisms
WO2006026654A2 (en) * 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
EP1794328B1 (de) 2004-09-30 2015-05-27 Gen-Probe Incorporated Verfahren zum nachweis und zur quantifizierung von hiv-1
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
CA2491067A1 (en) 2004-12-24 2006-06-24 Stichting Katholieke Universiteit Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer
AU2006214141B2 (en) 2005-02-18 2010-07-15 Gen-Probe Incorporated Sample preparation method incorporating an alkaline shock
CA2871777C (en) 2005-03-10 2015-07-28 Matthew J. Hayes System and methods for detecting multiple optical signals
US20060257902A1 (en) * 2005-03-25 2006-11-16 Ambion, Inc. Methods and compositions for depleting abundant RNA transcripts
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP2471805A3 (de) 2005-05-06 2013-01-16 Gen-Probe Incorporated Zusammensetzungen und Verfahren zur spezifischen Detektion von Nukleinsäuren der Viren Influenza A oder B
JP5187847B2 (ja) * 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド 核酸標的の捕獲方法
WO2006133385A2 (en) * 2005-06-06 2006-12-14 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of chlamydophila pneumoniae in a test sample
JP2009511086A (ja) 2005-10-17 2009-03-19 ジェン−プローブ・インコーポレーテッド レジオネラ・ニューモフィラ核酸を検出するための組成物及び方法
EP2479291A1 (de) * 2005-10-27 2012-07-25 Life Technologies Corporation Nucleinsäureverstärkung unter Verwendung nichtzufälliger Primer
EP2206779B1 (de) 2005-12-06 2013-02-13 Life Technologies Corporation Rückübertragungs-Primer und Verfahren zu deren Entwurf
CA2651946A1 (en) * 2006-05-12 2007-11-22 Gen-Probe Incorporated Compositions and methods to detect enterococci nucleic acid
AU2007267517B2 (en) * 2006-05-24 2012-12-20 Gen-Probe Incorporated Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe
AU2013201413B2 (en) * 2006-05-24 2014-05-29 Gen-Probe Incorporated Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe
PL2017356T3 (pl) 2006-06-06 2012-03-30 Gen Probe Inc Znakowane oligonukleotydy i ich zastosowanie w sposobach amplifikacji kwasu nukleinowego
CA2658105C (en) * 2006-08-01 2016-07-05 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
CA2673017C (en) 2006-12-21 2015-08-04 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1978111B1 (de) 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
EP2067867A1 (de) * 2007-12-03 2009-06-10 Siemens Aktiengesellschaft Verfahren zur Konzentration von Nukleinsäuremolekülen
JP5498954B2 (ja) 2007-12-21 2014-05-21 ジェン−プロウブ インコーポレイテッド 抗生剤耐性微生物の検出
JP5646455B2 (ja) 2008-04-21 2014-12-24 ジェン−プロウブ インコーポレイテッド チクングニヤウイルスを検出するための方法
EP2806037B1 (de) 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
CA2723917A1 (en) 2008-05-30 2009-12-30 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of salmonella
AU2009313808B2 (en) * 2008-11-14 2013-05-16 Gen-Probe Incorporated Compositions, kits and methods for detection of campylobacter nucleic acid
WO2010068473A1 (en) 2008-11-25 2010-06-17 Gen-Probe Incorporated Compositions and methods for detecting small rnas, and uses thereof
US20120100526A1 (en) * 2008-12-10 2012-04-26 Smiths Detection Inc. Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization
CA2746539A1 (en) 2008-12-30 2010-07-08 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of listeria
EP3705486B1 (de) 2009-02-26 2022-11-16 Gen-Probe Incorporated Assay zum nachweisen menschlicher parvoviren-nukleinsäure
US9212397B2 (en) 2009-06-23 2015-12-15 Gen-Probe Incorporated Compositions and methods for detecting nucleic acid from mollicutes
EP2449132B1 (de) 2009-07-01 2015-05-13 Gen-Probe Incorporated Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren
AU2010286368B2 (en) 2009-08-31 2015-09-03 Gen-Probe Incorporated Dengue virus assay
CA2787327C (en) 2010-01-22 2015-09-15 Damon Kittredge Getman Probes for detecting the presence of trichomonas vaginalis in a sample
EP3604558B1 (de) 2010-02-17 2022-10-12 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von atopobium-vaginae-nukleinsäure
US9206484B2 (en) 2010-04-21 2015-12-08 Gen-Probe Incorporated Compositions, methods and kits to detect herpes simplex virus nucleic acids
EP2593569B1 (de) 2010-07-12 2018-01-03 Gen-Probe Incorporated Zusammensetzungen und tests für den nachweis von nukleinsäuren des saisonalen h1 influenza a virus
CN103657754B (zh) 2010-07-23 2016-01-06 贝克曼考尔特公司 吸液管
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
EP2611932A2 (de) 2010-08-30 2013-07-10 Gen-Probe Incorporated Zusammensetzungen, verfahren und reaktionsmischungen für den nachweis von xenotropic murine leukemia virus-related virus (xmrv)
US9938590B2 (en) * 2010-09-16 2018-04-10 Gen-Probe Incorporated Capture probes immobilizable via L-nucleotide tail
EP2625297B1 (de) 2010-10-04 2018-10-10 Gen-Probe Prodesse, Inc. Zusammensetzungen, verfahren und kits für den nachweis von adenovirus-nukleinsäuren
EP2635703B1 (de) 2010-11-01 2018-03-21 Gen-Probe Incorporated Integriertes binden und amplifikation von zielnukleinsäuren zum sequenzieren
WO2012116308A1 (en) 2011-02-24 2012-08-30 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
JP6430826B2 (ja) * 2011-04-01 2018-11-28 オッカム・バイオラブズ・インコーポレイテッドOccam Biolabs, Inc. 無細胞病原体特異的核酸を検出する方法およびキット
WO2012149034A1 (en) 2011-04-25 2012-11-01 Gen-Probe Incorporated Compositions and methods for detecting bv-associated bacterial nucleic acid
ES2945966T3 (es) 2011-07-15 2023-07-11 Gen Probe Inc Composiciones y procedimiento para detectar ácido nucleico del parvovirus humano y ácido nucleico del virus de la hepatitis A
EP4219741A3 (de) 2011-09-06 2023-08-23 Gen-Probe Incorporated Geschlossene nukleinsäurestrukturen
EP3255155B1 (de) 2011-09-08 2019-04-24 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von bv-assoziierter bakterieller nukleinsäure
WO2013044097A1 (en) 2011-09-21 2013-03-28 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
EP2776844B1 (de) 2011-11-07 2020-09-30 Beckman Coulter, Inc. Probenbehälterdetektion
BR112014011048A2 (pt) 2011-11-07 2017-05-02 Beckman Coulter Inc braço robótico
BR112014011035A2 (pt) 2011-11-07 2017-06-13 Beckman Coulter, Inc. sistema de aliquotagem e fluxo de trabalho
CN104105969B (zh) 2011-11-07 2016-10-12 贝克曼考尔特公司 离心机系统和工作流程
CN103975245A (zh) 2011-11-07 2014-08-06 贝克曼考尔特公司 用于试样输送系统的磁阻尼
EP2776848B1 (de) 2011-11-07 2019-12-25 Beckman Coulter, Inc. System und verfahren zum transport von probenbehältern
DE102011120550B4 (de) 2011-12-05 2013-11-07 Gen-Probe Prodesse, Inc. Zusammensetzungen, Verfahren und Kits zur Detektion von Adenovirusnukleinsäuren
WO2013116774A1 (en) 2012-02-01 2013-08-08 Gen-Probe Incorporated Asymmetric hairpin target capture oligomers
CA2865281C (en) 2012-02-24 2021-11-23 Gen-Probe Prodesse, Inc. Detection of shiga toxin genes in bacteria
EP2824180B1 (de) * 2012-03-05 2019-04-03 NGK Insulators, Ltd. Verfahren zur detektion einer zielnucleinsäure
AU2013205110B2 (en) 2012-04-24 2016-10-13 Gen-Probe Incorporated Compositions, Methods and Kits to Detect Herpes Simplex Virus Nucleic Acids
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
AU2013205087B2 (en) 2012-07-13 2016-03-03 Gen-Probe Incorporated Method for detecting a minority genotype
MX364957B (es) 2012-08-14 2019-05-15 10X Genomics Inc Composiciones y metodos para microcapsulas.
US11591637B2 (en) * 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014036369A1 (en) 2012-08-30 2014-03-06 Gen-Probe Incorporated Multiphase nucleic acid amplification
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
AU2013205090B2 (en) 2012-12-07 2016-07-28 Gen-Probe Incorporated Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
BR112015019159A2 (pt) 2013-02-08 2017-07-18 10X Genomics Inc geração de código de barras de polinucleotídeos
US9400285B2 (en) 2013-03-15 2016-07-26 Abbot Laboratories Automated diagnostic analyzers having vertically arranged carousels and related methods
ES2901756T3 (es) 2013-03-15 2022-03-23 Abbott Lab Analizadores de diagnóstico automatizados que tienen sistemas de pista accesibles por la parte trasera y métodos relacionados
JP6165961B2 (ja) 2013-03-15 2017-07-19 アボット・ラボラトリーズAbbott Laboratories 前処理カルーセルを有する診断分析装置および関連方法
US10053742B2 (en) 2013-08-14 2018-08-21 Gen-Probe Incorporated Compositions and methods for detecting HEV nucleic acid
WO2015070173A1 (en) * 2013-11-08 2015-05-14 Isis Pharmaceuticals, Inc. Compounds and methods for detecting oligonucleotides
DE102015017080B3 (de) 2014-02-28 2024-01-04 Gen-Probe Incorporated Verfahren zur Isolierung von Nukleinsäure von zytologischen Proben in flüssigen Konservierungsmitteln, die Formaldehyd enthalten
EP3670664B1 (de) 2014-02-28 2022-09-07 Gen-Probe Incorporated Verfahren zur isolierung von nukleinsäure aus proben in flüssigkeitsbasierten zytologischen konservierungsmitteln mit formaldehyd
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
JP6767374B2 (ja) 2014-10-20 2020-10-14 ジェン−プローブ・インコーポレーテッド 赤血球溶解溶液
EP3242956B1 (de) 2015-01-09 2020-06-17 Gen-Probe Incorporated Verfahren und zusammensetzungen zur diagnose von bakterieller vaginose
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
US10550438B2 (en) 2015-03-16 2020-02-04 Gen-Probe Incorporated Methods and compositions for detecting bacterial nucleic acid
CN106868098A (zh) * 2015-12-10 2017-06-20 益善生物技术股份有限公司 循环肿瘤细胞分型鉴定试剂盒
CA3010232A1 (en) 2016-01-04 2017-07-13 Gen-Probe Incorporated Methods and compositions for detecting candida species
DE202017007129U1 (de) 2016-04-27 2019-08-29 Gen-Probe Incorporated Lysereagenz für Blutzellen
WO2017214511A2 (en) 2016-06-10 2017-12-14 Gen-Probe Incorporated Compositions and methods for detecting zika virus nucleic acid
US20180016623A1 (en) * 2016-07-15 2018-01-18 Northwestern University Compositions and methods for detecting nucleic acids in sputum
AU2017325833B2 (en) 2016-09-15 2022-06-16 Abbott Laboratories Devices and methods for sample analysis
EP3529381B1 (de) 2016-10-19 2022-07-06 Gen-Probe Incorporated Mischungen und verfahren zur detektion und quantifizierung von hepatitis c viren
EP3541959A1 (de) 2016-11-21 2019-09-25 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis oder zur quantifizierung des hepatitis-b-virus
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10799870B2 (en) 2017-03-03 2020-10-13 Gen-Probe Incorporated Evaporation-limiting inserts for reagent containers and related methods of use
EP4212635A1 (de) 2017-03-24 2023-07-19 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von rsv a in proben
JP6956800B2 (ja) 2017-03-25 2021-11-02 ジェン−プローブ・インコーポレーテッド アデノウイルス、メタニューモウイルス、および/またはライノウイルス核酸を検出するための組成物、方法、およびキット
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4230746A3 (de) 2017-05-26 2023-11-01 10X Genomics, Inc. Einzelzellanalyse von transposase-zugänglichem chromatin
JP7292217B2 (ja) 2017-06-07 2023-06-16 ジーイーエヌ-プローブ・インコーポレーテッド 試料中のバベシア種の核酸の検出
CN111032209A (zh) 2017-07-10 2020-04-17 简·探针公司 分析系统和方法
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CA3082909C (en) 2017-11-17 2023-07-25 Gen-Probe Incorporated Compositions and methods for detecting c1orf43 nucleic acid
CA3089078A1 (en) 2018-01-29 2019-08-01 Gen-Probe Incorporated Analytical systems and methods
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN112654721A (zh) 2018-06-13 2021-04-13 简·探针公司 用于检测b群链球菌核酸的组合物和方法
EP3814496B1 (de) 2018-06-28 2023-11-22 Gen-Probe Incorporated Probenvorbereitungsverfahren und -system
WO2020010305A1 (en) * 2018-07-05 2020-01-09 The Board Of Regents Of The University Of Oklahoma Gene signatures for cancer characterization and methods of use
WO2020014400A1 (en) 2018-07-10 2020-01-16 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
EP3830302B1 (de) 2018-08-01 2022-10-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von nukleinsäuren des epstein-barr-virus
WO2020033557A1 (en) 2018-08-08 2020-02-13 Gen-Probe Incorporated Compositions, methods and kits for detecting mycoplasma genitalium
US20220074002A1 (en) 2018-08-21 2022-03-10 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
AU2019324196A1 (en) 2018-08-24 2021-03-18 Gen-Probe Incorporated Compositions and methods for detecting bacterial nucleic acid and diagnosing bacterial vaginosis
EP3856934A2 (de) 2018-09-27 2021-08-04 Gen-Probe Incorporated Zusammensetzungen und verfahren zur detektion von bordetella-pertussis- und bordetella-parapertussis-nukleinsäure
CA3112342A1 (en) 2018-10-01 2020-04-09 Gen-Probe Incorporated Compositions and methods for amplifying or detecting varicella-zoster virus
WO2020086546A1 (en) 2018-10-22 2020-04-30 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human polyomavirus bk virus
TW202030333A (zh) 2018-12-20 2020-08-16 美商簡 探針公司 用於檢測瘧原蟲物種核酸之組成物及方法
EP3914732A1 (de) 2019-01-25 2021-12-01 Gen-Probe Incorporated Nachweis von arzneimittelresistentem mycoplasma genitalium
AU2020299621A1 (en) 2019-07-03 2022-02-24 Gen-Probe Incorporated Oligonucleotides for use in determining the presence of Trichomonas vaginalis in a sample.
US20220298548A1 (en) 2019-08-23 2022-09-22 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
JP2023516683A (ja) 2020-03-04 2023-04-20 ジェン-プローブ・インコーポレーテッド SARS-CoV-2核酸を検出するための組成物および方法
CN115698325A (zh) 2020-05-07 2023-02-03 盖立复诊断解决方案公司 用于检测SARS-CoV-2核酸的方法和组合物
EP4182482A1 (de) 2020-07-17 2023-05-24 Gen-Probe Incorporated Nachweis von makrolidresistentem mycoplasma genitalium
WO2022241291A1 (en) 2021-05-14 2022-11-17 Gen-Probe Incorporated Compositions and methods for detecting human adenovirus nucleic acid
CA3220932A1 (en) 2021-07-01 2023-01-05 Gen-Probe Incorporated Enzyme formulations and reaction mixtures for nucleic acid amplification
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4554088A (en) 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
GB8401368D0 (en) 1984-01-19 1984-02-22 Amersham Int Plc Assay method
EP0177497A4 (de) 1984-04-05 1987-07-06 Life Technologies Inc Immobilisierung von nukleinsäuren.
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4755458A (en) * 1984-08-30 1988-07-05 Enzo Biochem, Inc. Composition and method for the detection of the presence of a polynucleotide sequence of interest
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5386022A (en) 1985-03-28 1995-01-31 Hoffman-La Roche Inc. Primes and probes for the amplification and detection of aids associated nucleic acids
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US5273882A (en) * 1985-06-13 1993-12-28 Amgen Method and kit for performing nucleic acid hybridization assays
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4882269A (en) 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
ATE88762T1 (de) * 1986-08-11 1993-05-15 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
US5750338A (en) * 1986-10-23 1998-05-12 Amoco Corporation Target and background capture methods with amplification for affinity assays
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
IE72468B1 (en) * 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
US5283174A (en) * 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5639604A (en) 1987-09-21 1997-06-17 Gen-Probe Incorporated Homogeneous protection assay
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
AU2735988A (en) * 1987-12-21 1989-07-13 Amoco Corporation Target and background capture methods with amplification for affinity assays
FR2625511B1 (fr) * 1987-12-31 1990-05-18 Sanofi Sa Procede d'obtention in vitro de tissus reconstitues
JPH03504199A (ja) * 1988-05-10 1991-09-19 イー・アイ・デユポン・ド・ネモアース・アンド・コンパニー 迅速な核酸検出法
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA2002076A1 (en) * 1988-11-21 1990-05-21 Brent A. Burdick Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
EP0446260B1 (de) 1988-11-21 1994-03-02 Dynal A.S. Sonden aus nukleinsäuren
US5449769A (en) * 1989-03-06 1995-09-12 Gen-Probe Incorporated Method and reagent for sulfurization of organophosphorous compounds
WO1990010716A1 (en) * 1989-03-10 1990-09-20 Gene-Trak Systems Immobilized oligonucleotide probes and uses therefor
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
US5656207A (en) * 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
ES2103795T3 (es) * 1989-12-04 1997-10-01 Microprobe Corp Mejora de la captura de un acido nucleico diana mediante el uso de oligonucleotidos unidos covalentemente a polimeros.
CA2032203C (en) 1989-12-29 2009-05-19 Christine L. Brakel Amplification capture assay
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
JP4372837B2 (ja) * 1992-08-04 2009-11-25 ジェン−プローブ・インコーポレイテッド 核酸配列増幅
JPH08503957A (ja) * 1993-02-19 1996-04-30 アイシス・ファーマシューティカルス・インコーポレーテッド シクロブチルアンチセンスオリゴヌクレオチド、その作成および使用方法
WO1995003430A1 (en) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
EP0648835A1 (de) * 1993-10-14 1995-04-19 The Procter & Gamble Company Verwendung von alkalischen Polyammoniumsalzen zur Verbesserung der Kationendichte von Textilweichmachern
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
EP0709466B1 (de) * 1994-10-28 2006-09-27 Gen-Probe Incorporated Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
US5731153A (en) * 1996-08-26 1998-03-24 The Regents Of The University Of California Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
US6060246A (en) 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
EP0975807B1 (de) * 1997-05-02 2006-09-27 Gen-Probe Incorporated Zwei-schritt hybridisierung und einfang von einem polynukleotid

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JP2002511745A (ja) 2002-04-16
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CA2287570C (en) 2008-10-28
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US6110678A (en) 2000-08-29
EP0975807A1 (de) 2000-02-02
JP4222635B2 (ja) 2009-02-12
CA2287570A1 (en) 1998-11-12
ATE340868T1 (de) 2006-10-15
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KR20010012175A (ko) 2001-02-15
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