DE69711893D1 - Elektroporationsgerät mit benutzerkonfigurierten impulsen - Google Patents

Elektroporationsgerät mit benutzerkonfigurierten impulsen

Info

Publication number
DE69711893D1
DE69711893D1 DE69711893T DE69711893T DE69711893D1 DE 69711893 D1 DE69711893 D1 DE 69711893D1 DE 69711893 T DE69711893 T DE 69711893T DE 69711893 T DE69711893 T DE 69711893T DE 69711893 D1 DE69711893 D1 DE 69711893D1
Authority
DE
Germany
Prior art keywords
cells
low voltage
duration
cell
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69711893T
Other languages
English (en)
Other versions
DE69711893T2 (de
Inventor
A Hofmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetronics Inc
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Publication of DE69711893D1 publication Critical patent/DE69711893D1/de
Application granted granted Critical
Publication of DE69711893T2 publication Critical patent/DE69711893T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • H03K3/02—Generators characterised by the type of circuit or by the means used for producing pulses
    • H03K3/53—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback
    • H03K3/57—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback the switching device being a semiconductor device
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
DE69711893T 1996-09-09 1997-01-27 Elektroporationsgerät mit benutzerkonfigurierten impulsen Expired - Fee Related DE69711893T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/709,615 US5869326A (en) 1996-09-09 1996-09-09 Electroporation employing user-configured pulsing scheme
PCT/US1997/001088 WO1998010515A1 (en) 1996-09-09 1997-01-27 Electroporation employing user-configured pulses

Publications (2)

Publication Number Publication Date
DE69711893D1 true DE69711893D1 (de) 2002-05-16
DE69711893T2 DE69711893T2 (de) 2002-11-14

Family

ID=24850605

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69711893T Expired - Fee Related DE69711893T2 (de) 1996-09-09 1997-01-27 Elektroporationsgerät mit benutzerkonfigurierten impulsen

Country Status (9)

Country Link
US (2) US5869326A (de)
EP (1) EP0925647B1 (de)
JP (1) JP3963955B2 (de)
KR (1) KR100420731B1 (de)
AT (1) ATE216155T1 (de)
CA (1) CA2261703A1 (de)
DE (1) DE69711893T2 (de)
ES (1) ES2175343T3 (de)
WO (1) WO1998010515A1 (de)

Families Citing this family (229)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5636166A (en) * 1995-12-05 1997-06-03 Micron Quantum Devices, Inc. Apparatus for externally timing high voltage cycles of non-volatile memory system
US5914613A (en) * 1996-08-08 1999-06-22 Cascade Microtech, Inc. Membrane probing system with local contact scrub
US6241701B1 (en) 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
US6216034B1 (en) 1997-08-01 2001-04-10 Genetronics, Inc. Method of programming an array of needle electrodes for electroporation therapy of tissue
US6055453A (en) 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US6135990A (en) * 1997-12-17 2000-10-24 University Of South Florida Electroporation device and method
US6778853B1 (en) 1997-12-17 2004-08-17 University Of South Florida Electroporation device
EP1086239A1 (de) * 1998-06-08 2001-03-28 Valentis Inc. Formulierungen für die elektroporation
AU760619B2 (en) * 1998-06-26 2003-05-22 Genetronics, Inc. Synergism of photodynamic and electropermeation effects on cell vitality as a novel cytotoxic agent
US7922709B2 (en) 1998-07-13 2011-04-12 Genetronics, Inc. Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
US6678556B1 (en) * 1998-07-13 2004-01-13 Genetronics, Inc. Electrical field therapy with reduced histopathological change in muscle
EP1100579B1 (de) 1998-07-13 2015-09-02 Inovio Pharmaceuticals, Inc. Auf haut und muskel gerrichtete gentherapie durch ein pulsierendes elektrisches feld
EP1102608A4 (de) 1998-07-13 2008-09-17 Genetronics Inc Methode und gerät zur elektrisch unterstützten oberflächlichen auftragung von kosmetikmitteln
US6256882B1 (en) * 1998-07-14 2001-07-10 Cascade Microtech, Inc. Membrane probing system
US6192270B1 (en) * 1998-08-14 2001-02-20 Genetronics, Inc. Apparatus and method for the delivery of drugs and genes into tissue
US6532386B2 (en) 1998-08-31 2003-03-11 Johnson & Johnson Consumer Companies, Inc. Electrotransort device comprising blades
US6150148A (en) * 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US6937890B2 (en) * 1998-12-17 2005-08-30 University Of South Florida Nonpenetrating electroporation device
US6314316B1 (en) * 1998-12-17 2001-11-06 University Of South Florida Nonpenetrating electroporation device and method
EP1171195B1 (de) 1999-04-16 2005-03-16 Johnson & Johnson Consumer Companies, Inc. Vorrichtung zur iontophoretischen verabreichung von medikamenten mit internen sensoren
US6578264B1 (en) * 1999-06-04 2003-06-17 Cascade Microtech, Inc. Method for constructing a membrane probe using a depression
WO2001001949A1 (en) 1999-07-01 2001-01-11 Johnson And Johnson Consumer Companies, Inc. Cleansing compositions
US20050009717A1 (en) * 1999-07-01 2005-01-13 Lukenbach Elvin R. Foaming make-up removing cleansing compositions
US7074747B1 (en) 1999-07-01 2006-07-11 Johnson & Johnson Consumer Companies, Inc. Cleansing compositions
US6762158B2 (en) 1999-07-01 2004-07-13 Johnson & Johnson Consumer Companies, Inc. Personal care compositions comprising liquid ester mixtures
WO2001055294A1 (fr) * 2000-01-27 2001-08-02 The Mollennium Laboratories Dispositif, auxiliaire, et procede de transfert moleculaire
US6868289B2 (en) * 2002-10-02 2005-03-15 Standen Ltd. Apparatus for treating a tumor or the like and articles incorporating the apparatus for treatment of the tumor
US7016725B2 (en) * 2001-11-06 2006-03-21 Standen Ltd. Method and apparatus for destroying dividing cells
US7599746B2 (en) 2000-02-17 2009-10-06 Standen Ltd Apparatus and method for preventing the spread of cancerous metastases and for elimination of metastases
US7146210B2 (en) * 2000-02-17 2006-12-05 Standen Ltd. Apparatus and method for optimizing tumor treatment efficiency by electric fields
US7136699B2 (en) * 2002-10-02 2006-11-14 Standen, Ltd. Apparatus for destroying dividing cells
US7089054B2 (en) * 2002-10-02 2006-08-08 Standen Ltd. Apparatus and method for treating a tumor or the like
US8175698B2 (en) * 2000-02-17 2012-05-08 Novocure Ltd. Treating bacteria with electric fields
US8447395B2 (en) * 2000-02-17 2013-05-21 Novocure Ltd Treating bacteria with electric fields
EP2335776A1 (de) * 2000-02-17 2011-06-22 Yoram Palti Verfahren und vorrichtung zur zerstörung von teilenden zellen
US6714816B1 (en) * 2000-02-22 2004-03-30 University Of South Florida Electroporation and electrophoresis system and method for achieving molecular penetration into cells in vivo
US8024048B2 (en) * 2000-03-13 2011-09-20 Ionix Medical Inc. Method and device for treating cancer with electrical therapy in conjunction with chemotherapeutic agents and radiation therapy
US7742811B2 (en) * 2000-03-13 2010-06-22 Onco Stim Implantable device and method for the electrical treatment of cancer
CA2409603A1 (en) 2000-05-22 2001-11-29 Merck & Company, Inc. System and method for assessing the performance of a pharmaceutical agent delivery system
DE10031179A1 (de) * 2000-06-27 2002-01-31 Amaxa Gmbh Verfahren zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
US6914423B2 (en) 2000-09-05 2005-07-05 Cascade Microtech, Inc. Probe station
US6965226B2 (en) 2000-09-05 2005-11-15 Cascade Microtech, Inc. Chuck for holding a device under test
DE10143173A1 (de) 2000-12-04 2002-06-06 Cascade Microtech Inc Wafersonde
US6645757B1 (en) 2001-02-08 2003-11-11 Sandia Corporation Apparatus and method for transforming living cells
DE10119901A1 (de) * 2001-04-23 2002-10-24 Amaxa Gmbh Schaltungsanordnung zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
US6733485B1 (en) 2001-05-25 2004-05-11 Advanced Bionics Corporation Microstimulator-based electrochemotherapy methods and systems
US7127284B2 (en) * 2001-06-11 2006-10-24 Mercator Medsystems, Inc. Electroporation microneedle and methods for its use
US20040204669A1 (en) * 2001-07-05 2004-10-14 Hofmann Gunter A. Apparatus for electroporation mediated delivery for drugs and genes
KR20030012995A (ko) * 2001-08-06 2003-02-14 주식회사 토이랩 Ｄｃ 전원을 사용한 전기천공기
WO2003052435A1 (en) * 2001-08-21 2003-06-26 Cascade Microtech, Inc. Membrane probing system
US20030170898A1 (en) * 2001-12-04 2003-09-11 Gundersen Martin A. Method for intracellular modifications within living cells using pulsed electric fields
US20030129716A1 (en) * 2001-12-06 2003-07-10 Bio-Rad Laboratories, Inc. Automatic electroporation optimization system
CA2476452A1 (en) * 2002-02-15 2003-08-28 Zycos Inc. Electroporation methods for introducing bioactive agents into cells
PT1480720T (pt) * 2002-03-07 2018-11-23 Inovio Pharmaceuticals Inc Conjunto de elétrodos para eletroporação de corrente constante e uso
US7245963B2 (en) * 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US8209006B2 (en) * 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
US20030204161A1 (en) * 2002-04-25 2003-10-30 Bozidar Ferek-Petric Implantable electroporation therapy device and method for using same
EP1515775A4 (de) 2002-05-07 2010-03-03 Oncostim Inc Verfahren und vorrichtung zur behandlung von krebs mit elektrotherapie in verbindung mit chemotherapeutischen mitteln und strahlentherapie
US6929949B1 (en) 2002-06-14 2005-08-16 University Of South Florida Corona ion generating method and apparatus for the manipulation of molecules and biological cells
US7057404B2 (en) 2003-05-23 2006-06-06 Sharp Laboratories Of America, Inc. Shielded probe for testing a device under test
US7492172B2 (en) 2003-05-23 2009-02-17 Cascade Microtech, Inc. Chuck for holding a device under test
US6969604B1 (en) 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US7250626B2 (en) 2003-10-22 2007-07-31 Cascade Microtech, Inc. Probe testing structure
US8500713B2 (en) * 2003-10-29 2013-08-06 Medtronic, Inc. Implantable electroporation therapy device and method for using same
US7187188B2 (en) 2003-12-24 2007-03-06 Cascade Microtech, Inc. Chuck with integrated wafer support
GB2425844B (en) 2003-12-24 2007-07-11 Cascade Microtech Inc Active wafer probe
US20050222646A1 (en) * 2004-04-06 2005-10-06 Kai Kroll Method and device for treating cancer with modified output electrical therapy
WO2006080934A2 (en) * 2004-04-19 2006-08-03 Invitrogen Corporation Electroporation apparatus and methods
CN1976738B (zh) * 2004-04-23 2010-09-01 诺沃库勒有限公司 使用不同频率的电场治疗肿瘤等
CA2565793A1 (en) * 2004-05-06 2005-12-08 The Trustees Of Dartmouth College Method and apparatus for bacterial transformation by electroporation with waveforms incorporating pulsed rf between 3 and 125 mhz
ES2465467T3 (es) * 2004-06-14 2014-06-05 Lonza Cologne Ag Procedimiento y disposición de circuito para el tratamiento de material biológico
TW200613554A (en) 2004-06-17 2006-05-01 Wyeth Corp Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV
US7368927B2 (en) * 2004-07-07 2008-05-06 Cascade Microtech, Inc. Probe head having a membrane suspended probe
US7420381B2 (en) 2004-09-13 2008-09-02 Cascade Microtech, Inc. Double sided probing structures
US7776373B2 (en) * 2004-11-19 2010-08-17 Eteka Llc Apparatus and method for the enhancement of food properties and food prepared therefrom
US7715921B2 (en) * 2004-12-07 2010-05-11 Standen Ltd. Electrodes for applying an electric field in-vivo over an extended period of time
CA2594231C (en) * 2004-12-27 2016-04-19 Standen Ltd. Treating a tumor or the like with electric fields at different orientations
SG123643A1 (en) * 2004-12-28 2006-07-26 Nanyan Polytechnic Programmable apparatus and method for optimizing and real time monitoring of gene transfection basedon user configured arbitrary waveform pulsing tra in
US7656172B2 (en) 2005-01-31 2010-02-02 Cascade Microtech, Inc. System for testing semiconductors
US7535247B2 (en) 2005-01-31 2009-05-19 Cascade Microtech, Inc. Interface for testing semiconductors
DE502005002350D1 (de) * 2005-07-07 2008-02-07 Amaxa Ag Verfahren zur Behandlung geringer Volumina mit elektrischem Strom
ES2534488T3 (es) 2005-10-03 2015-04-23 Novocure Ltd. Características de optimización de un campo eléctrico para aumentar el efecto del campo sobre las células que proliferan
FR2893256B1 (fr) * 2005-11-14 2008-08-15 Yves Scherman Generateur d'impulsions electriques unipolaires.
US7824870B2 (en) * 2006-01-03 2010-11-02 Alcon, Inc. System for dissociation and removal of proteinaceous tissue
US20070232984A1 (en) * 2006-03-30 2007-10-04 Michael Lovell Hand-held electrical stimulation device
US8019414B2 (en) 2006-04-05 2011-09-13 Novocure Ltd. Treating cancer using electromagnetic fields in combination with other treatment regimens
US7403028B2 (en) 2006-06-12 2008-07-22 Cascade Microtech, Inc. Test structure and probe for differential signals
US7764072B2 (en) 2006-06-12 2010-07-27 Cascade Microtech, Inc. Differential signal probing system
US7723999B2 (en) 2006-06-12 2010-05-25 Cascade Microtech, Inc. Calibration structures for differential signal probing
US20080012578A1 (en) * 2006-07-14 2008-01-17 Cascade Microtech, Inc. System for detecting molecular structure and events
CN105457157B (zh) 2006-10-17 2020-04-14 因诺维奥制药公司 电穿孔装置及用其进行哺乳动物细胞电穿孔的方法
EP3231480A1 (de) 2007-03-06 2017-10-18 Novocure Ltd. Krebsbehandlung mithilfe elektromagnetischer felder in kombination mit einer photodynamischen therapie
US7876114B2 (en) 2007-08-08 2011-01-25 Cascade Microtech, Inc. Differential waveguide probe
PT2183024T (pt) * 2007-08-14 2019-08-01 Novocure Ltd Tratando parasitas com campos elétricos
DE102007056488A1 (de) 2007-11-22 2009-07-23 Biontex Laboratories Gmbh Steigerung von Transfektionseffizienzen nicht-viraler Genliefersysteme durch Blockierung des angeborenen Immunsystems
DE102008016275A1 (de) 2008-03-28 2009-11-19 Biontex Laboratories Gmbh Verbesserung von Transfektionsergebnissen nicht-viraler Genliefersysteme durch Blockierung des angeborenen Immunsystems
DE102008023913A1 (de) 2008-05-16 2009-11-19 Biontex Laboratories Gmbh Verbesserung von Transfektionsergebnissen nicht-viraler Genliefersysteme durch Beeinflussung des angeborenen Immunsystems
WO2009065618A2 (de) 2007-11-22 2009-05-28 Biontex Laboratories Gmbh Verbesserung von transfektionsergebnissen nicht-viraler genliefersysteme durch beeinflussung des angeborenen immunsystems
US20110045001A1 (en) * 2008-03-28 2011-02-24 Biontex Laboratories Gmbh Transfection results of non-viral gene delivery systems by influencing of the innate immune system
US10842555B2 (en) * 2008-08-20 2020-11-24 Prostacare Pty Ltd Catheter for treating tissue with non-thermal ablation
US8222909B2 (en) * 2008-08-22 2012-07-17 Bio-Rad Laboratories, Inc. Electroporation system with dial-in time constant
US7888957B2 (en) * 2008-10-06 2011-02-15 Cascade Microtech, Inc. Probing apparatus with impedance optimized interface
US8410806B2 (en) 2008-11-21 2013-04-02 Cascade Microtech, Inc. Replaceable coupon for a probing apparatus
US8319503B2 (en) 2008-11-24 2012-11-27 Cascade Microtech, Inc. Test apparatus for measuring a characteristic of a device under test
US20110118729A1 (en) * 2009-11-13 2011-05-19 Alcon Research, Ltd High-intensity pulsed electric field vitrectomy apparatus with load detection
US20110118734A1 (en) * 2009-11-16 2011-05-19 Alcon Research, Ltd. Capsularhexis device using pulsed electric fields
US20110135626A1 (en) * 2009-12-08 2011-06-09 Alcon Research, Ltd. Localized Chemical Lysis of Ocular Tissue
US20110144638A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Shockwave-Induced Tissue Disruption
US20110144562A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Pharmacological Treatment of Ocular Tissue Using High-Intensity Pulsed Electrical Fields
WO2011081897A1 (en) * 2009-12-15 2011-07-07 Alcon Research, Ltd. High-intensity pulsed electric field vitrectomy apparatus
JP4713671B1 (ja) * 2010-01-22 2011-06-29 ネッパジーン株式会社 エレクトロポレーション法による外来遺伝子導入法
EP2399984B1 (de) 2010-06-22 2013-03-20 Lonza Cologne GmbH Verfahren und Elektrodenanordnung zur Behandlung von adhärenten Zellen
US8546979B2 (en) 2010-08-11 2013-10-01 Alcon Research, Ltd. Self-matching pulse generator with adjustable pulse width and pulse frequency
CA2809360A1 (en) 2010-09-27 2012-04-05 China Agricultural University Combined antigen and dna vaccine for preventing and treating autoimmune diseases
WO2012158132A1 (en) 2011-05-18 2012-11-22 Eteka Llc Apparatus for the enhancement of food properties by electroporation or pulsed electric fields
CN103239734B (zh) 2012-02-10 2016-02-24 北京艾棣维欣生物技术有限公司 用于预防和/或治疗呼吸道合胞病毒感染的疫苗
JP2013198637A (ja) * 2012-03-26 2013-10-03 Toshiyuki Moriizumi エレクロトポレーター用電源
ES2757623T3 (es) 2012-07-25 2020-04-29 Broad Inst Inc Proteínas de unión a ADN inducibles y herramientas de perturbación genómica y aplicaciones de las mismas
JP2015534862A (ja) * 2012-11-14 2015-12-07 インターナショナル・サイエンティフィック・プロプライエタリー・リミテッド 経皮デリバリーデバイスを制御するための装置、システム、方法、コンピュータプログラム、および、データ信号
JP6523174B2 (ja) 2012-12-13 2019-05-29 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア Ｗｔ１ワクチン
US10364451B2 (en) 2013-05-30 2019-07-30 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
EP3003342B1 (de) 2013-05-30 2022-01-26 Duke University Enzymkatalysierte synthese von ortsspezifischen und stöchiometrischen biomolekül-polymer-konjugaten
US10392611B2 (en) 2013-05-30 2019-08-27 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
KR20160066033A (ko) 2013-10-07 2016-06-09 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 어쥬번트로서 인터류킨-33을 갖는 백신
US10370664B2 (en) 2013-11-07 2019-08-06 University Of Southern California Use of IKK epsilon inhibitors to activate NFAT and T cell response
EP3068427A1 (de) 2013-11-14 2016-09-21 The Trustees Of The University Of Pennsylvania Hiv-1-env-dna-impfstoff plus proteinboost
AU2014354797C1 (en) 2013-11-29 2018-02-01 Inovio Pharmaceuticals, Inc. MERS-CoV vaccine
EP3092004A4 (de) 2014-01-06 2017-02-22 The Trustees Of The University Of Pennsylvania Pd1- und pdl1-antikörper und impfstoffkombinationen und verwendung davon zur immuntherapie
US9708597B2 (en) * 2014-01-17 2017-07-18 General Electric Company Electric pulse generation systems using capacitive coupling
US9593219B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spin coating (IIa)
US9193835B1 (en) 2014-05-30 2015-11-24 Pall Corporation Self-assembling polymers—IV
US9598543B2 (en) 2014-05-30 2017-03-21 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (VIa)
US9169361B1 (en) 2014-05-30 2015-10-27 Pall Corporation Self-assembling polymers—VI
US9328206B2 (en) 2014-05-30 2016-05-03 Pall Corporation Self-assembling polymers—III
US9593218B2 (en) 2014-05-30 2017-03-14 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (IIIa)
US9593217B2 (en) 2014-05-30 2017-03-14 Pall Corporation Self-assembled structure and membrane comprising block copolymer and process for producing the same by spin coating (Va)
US9604181B2 (en) 2014-05-30 2017-03-28 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spray coating (IIc)
US9592477B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by hybrid casting (Ib)
US9765171B2 (en) 2014-05-30 2017-09-19 Pall Corporation Self-assembling polymers—V
US9592476B2 (en) 2014-05-30 2017-03-14 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by hybrid casting (IIb)
US9616395B2 (en) 2014-05-30 2017-04-11 Pall Corportaion Membrane comprising self-assembled block copolymer and process for producing the same by spray coating (Ic)
US9163122B1 (en) 2014-05-30 2015-10-20 Pall Corporation Self-assembling polymers—II
US9162189B1 (en) 2014-05-30 2015-10-20 Pall Corporation Membrane comprising self-assembled block copolymer and process for producing the same by spin coating (Ia)
US9309367B2 (en) 2014-06-30 2016-04-12 Pall Corporation Membranes comprising cellulosic material and hydrophilic block copolymer (V)
US9303133B2 (en) 2014-06-30 2016-04-05 Pall Corporation Hydrophilic membranes and method of preparation thereof (IV)
US9718924B2 (en) 2014-06-30 2017-08-01 Pall Corporation Hydrophilic block copolymers and membranes prepared therefrom (II)
US9394407B2 (en) 2014-06-30 2016-07-19 Pall Corporation Hydrophilic block copolymers and membranes prepared therefrom (I)
US9962662B2 (en) 2014-06-30 2018-05-08 Pall Corporation Fluorinated polymer and use thereof in the preparation of hydrophilic membranes (vi)
US9260569B2 (en) 2014-06-30 2016-02-16 Pall Corporation Hydrophilic block copolymers and method of preparation thereof (III)
US9254466B2 (en) 2014-06-30 2016-02-09 Pall Corporation Crosslinked cellulosic membranes
EP3200831A4 (de) 2014-10-01 2018-02-21 The Trustees Of The University Of Pennsylvania Impfstoffe mit einem antigen und interleukin-21 als adjuvans
WO2016094874A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
KR20170106453A (ko) 2015-01-29 2017-09-20 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 관문 억제제 및 백신 조합 및 면역요법을 위한 이들의 사용
US11160976B2 (en) 2015-02-17 2021-11-02 Eunsung Global Corp. Skin treatment device using needles
KR101740950B1 (ko) 2015-02-17 2017-06-15 주식회사 은성글로벌 니들을 이용한 피부 치료장치
WO2016154530A1 (en) 2015-03-26 2016-09-29 Duke University Targeted therapeutic agents comprising multivalent protein-biopolymer fusions
KR102575342B1 (ko) 2015-06-18 2023-09-05 더 브로드 인스티튜트, 인코퍼레이티드 표적외 효과를 감소시키는 crispr 효소 돌연변이
AU2016301391B2 (en) 2015-08-04 2022-07-28 Duke University Genetically encoded intrinsically disordered stealth polymers for delivery and methods of using same
US20190233814A1 (en) 2015-12-18 2019-08-01 The Broad Institute, Inc. Novel crispr enzymes and systems
EP3393500B1 (de) 2015-12-21 2021-10-13 Duke University Polymerkonjugate mit verringerter antigenizität
US11752213B2 (en) 2015-12-21 2023-09-12 Duke University Surfaces having reduced non-specific binding and antigenicity
US11246924B2 (en) 2016-04-01 2022-02-15 Duke University Alpha-helical peptide nanofibers as a self-adjuvanting vaccine platform
SG10202010311SA (en) 2016-04-19 2020-11-27 Broad Inst Inc Novel Crispr Enzymes and Systems
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
AU2017253107B2 (en) 2016-04-19 2023-07-20 Massachusetts Institute Of Technology CPF1 complexes with reduced indel activity
WO2017190147A1 (en) 2016-04-29 2017-11-02 Inovio Pharmaceuticals, Inc. The in vivo use of chondroitinase and/or hyaluronidase to enhance delivery of an agent
US11467156B2 (en) 2016-06-01 2022-10-11 Duke University Nonfouling biosensors
WO2018005873A1 (en) 2016-06-29 2018-01-04 The Broad Institute Inc. Crispr-cas systems having destabilization domain
US10233419B2 (en) 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
RU2019110848A (ru) 2016-09-14 2020-10-15 Дьюк Юниверсити Наночастицы на основе триблочных полипептидов для доставки гидрофильных лекарственных средств
EP3515928A4 (de) 2016-09-23 2020-04-01 Duke University Unstrukturierte nichtrepititive polypeptide mit lcst-verhalten
US10376495B2 (en) 2016-11-23 2019-08-13 University Of South Florida Small molecules that mimic or antagonize actions of granulocyte colony-stimulating-factor (G-CSF)
BR112019012924A2 (pt) 2016-12-22 2019-12-10 Ohio State Innovation Foundation microestruturas interpenetrantes para liberação de carga com base em nanocanal
US11648200B2 (en) 2017-01-12 2023-05-16 Duke University Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly
US10813935B2 (en) 2017-02-23 2020-10-27 Transgenex Nanobiotech, Inc. Methods and compositions for treating drug resistance in cancer
US11318155B2 (en) 2017-02-24 2022-05-03 University Of South Florida Hsp90 activator Aha1 drives production of pathological tau aggregates
US10272052B2 (en) 2017-02-24 2019-04-30 University Of South Florida Compositions and methods for the treatment of tauopathies
US20200071773A1 (en) 2017-04-12 2020-03-05 Massachusetts Eye And Ear Infirmary Tumor signature for metastasis, compositions of matter methods of use thereof
JP6518971B2 (ja) * 2017-05-12 2019-05-29 株式会社ベックス エレクトロポレーター用電源
WO2018213320A1 (en) 2017-05-15 2018-11-22 Duke University Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents
CN111417727A (zh) 2017-05-18 2020-07-14 博德研究所 用于靶向核酸编辑的系统、方法和组合物
EP3638218A4 (de) 2017-06-14 2021-06-09 The Broad Institute, Inc. Zusammensetzungen und verfahren zum targeting von komplementierender komponente 3 zur hemmung des tumorwachstums
CN111328290A (zh) 2017-06-26 2020-06-23 博德研究所 用于靶向核酸编辑的基于crispr/cas-腺嘌呤脱氨酶的组合物、系统和方法
WO2019006374A1 (en) 2017-06-30 2019-01-03 Duke University ORDER AND DISORDER AS A DESIGN PRINCIPLE FOR STIMULI-SENSITIVE BIOPOLYMER NETWORKS
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
CN111511388A (zh) 2017-09-21 2020-08-07 博德研究所 用于靶向核酸编辑的系统、方法和组合物
EP3692152A4 (de) 2017-10-04 2021-12-01 The Broad Institute, Inc. Verfahren und zusammensetzungen zur veränderung der funktion und struktur von chromatinschleifen und/oder -domänen
WO2019094983A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods and compositions for treating cancer by targeting the clec2d-klrb1 pathway
DE202018106744U1 (de) 2017-11-27 2019-05-13 Prostacare Pty Ltd Vorrichtung für die Behandlung einer Prostataerkrankung
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
WO2019168949A1 (en) 2018-02-28 2019-09-06 Prostacare Pty Ltd System for managing high impedance changes in a non-thermal ablation system for bph
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
WO2019210268A2 (en) 2018-04-27 2019-10-31 The Broad Institute, Inc. Sequencing-based proteomics
WO2019232542A2 (en) 2018-06-01 2019-12-05 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
WO2020010388A1 (en) * 2018-07-13 2020-01-16 International Scientific Pty Ltd A method and apparatus for user-controlled or practitioner-prescribed transdermal, intradermal or transappendageal delivery of therapeutic, cosmetic or skin care ingredients
US11649275B2 (en) 2018-08-02 2023-05-16 Duke University Dual agonist fusion proteins
CN113286884A (zh) 2018-08-07 2021-08-20 博德研究所 新型cas12b酶和系统
US20210317429A1 (en) 2018-08-20 2021-10-14 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
WO2020041384A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. 3-phenyl-2-cyano-azetidine derivatives, inhibitors of rna-guided nuclease activity
WO2020041387A1 (en) 2018-08-20 2020-02-27 The Brigham And Women's Hospital, Inc. Degradation domain modifications for spatio-temporal control of rna-guided nucleases
EP3852813A4 (de) 2018-09-18 2022-06-22 VNV Newco Inc. Lichtbogenbasierte kapside und deren verwendungen
US20220401460A1 (en) 2018-10-10 2022-12-22 Dana-Farber Cancer Institute, Inc. Modulating resistance to bcl-2 inhibitors
WO2020077236A1 (en) 2018-10-12 2020-04-16 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020081730A2 (en) 2018-10-16 2020-04-23 Massachusetts Institute Of Technology Methods and compositions for modulating microenvironment
WO2020090312A1 (ja) * 2018-10-29 2020-05-07 株式会社ベックス 気泡噴出方法、気泡噴出用電源装置、および、気泡噴出装置
AU2019379160A1 (en) 2018-11-14 2021-06-24 Massachusetts Institute Of Technology CRISPR system based droplet diagnostic systems and methods
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
US11384344B2 (en) 2018-12-17 2022-07-12 The Broad Institute, Inc. CRISPR-associated transposase systems and methods of use thereof
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
WO2020183984A1 (ja) * 2019-03-14 2020-09-17 株式会社ベックス エレクトロポレーションによるプロトプラストまたは藻類への物質導入方法、および、エレクトロポレーター用電源装置
US20220177863A1 (en) 2019-03-18 2022-06-09 The Broad Institute, Inc. Type vii crispr proteins and systems
US20220195514A1 (en) 2019-03-29 2022-06-23 The Broad Institute, Inc. Construct for continuous monitoring of live cells
US20220175848A1 (en) 2019-04-01 2022-06-09 The Broad Institute, Inc. Methods and compositions for cell therapy
WO2020206036A1 (en) 2019-04-01 2020-10-08 The Broad Institute, Inc. Novel nucleic acid modifier
US20220235340A1 (en) 2019-05-20 2022-07-28 The Broad Institute, Inc. Novel crispr-cas systems and uses thereof
WO2020236972A2 (en) 2019-05-20 2020-11-26 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
US20220243178A1 (en) 2019-05-31 2022-08-04 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
US11512314B2 (en) 2019-07-12 2022-11-29 Duke University Amphiphilic polynucleotides
US20220298501A1 (en) 2019-08-30 2022-09-22 The Broad Institute, Inc. Crispr-associated mu transposase systems
JP2022547233A (ja) * 2019-09-02 2022-11-10 ミライ メディカル リミテッド エレクトロポレーション装置および方法
WO2021074695A1 (en) 2019-10-16 2021-04-22 Avacta Life Sciences Limited PD-L1 INHIBITOR - TGFβ INHIBITOR BISPECIFIC DRUG MOIETIES.
IL295697A (en) 2020-02-25 2022-10-01 Inovio Pharmaceuticals Inc Vaccines against corona virus and methods of use
US11744885B2 (en) 2020-05-14 2023-09-05 Inovio Pharmaceuticals, Inc. Vaccines for recurrent respiratory papillomatosis and methods of using the same
GB202101299D0 (en) 2020-06-09 2021-03-17 Avacta Life Sciences Ltd Diagnostic polypetides and methods
IL298874A (en) 2020-06-12 2023-02-01 Univ Rochester Encoding and expression of ACE-tRNAs
WO2022234003A1 (en) 2021-05-07 2022-11-10 Avacta Life Sciences Limited Cd33 binding polypeptides with stefin a protein
TW202334196A (zh) 2021-10-07 2023-09-01 英商阿法克塔生命科學有限公司 Pd-l1結合多肽
TW202332694A (zh) 2021-10-07 2023-08-16 英商阿凡克塔生命科學公司 血清半衰期延長之ｐｄ－ｌ１結合多肽
WO2023150753A1 (en) 2022-02-07 2023-08-10 University Of Rochester Optimized sequences for enhanced trna expression or/and nonsense mutation suppression

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3607689A (en) * 1967-10-17 1971-09-21 Inoue K Power supply for large-surface electrochemical machining
US4063146A (en) * 1975-05-22 1977-12-13 Reliance Electric Company Digital firing control for a converter
US4408268A (en) * 1982-08-09 1983-10-04 General Electric Company Pulse modulated electronic voltage controller with smooth voltage output
IT1181297B (it) * 1984-04-19 1987-09-23 Icm Italiana Cost Metalmecc Dispositivo per il controllo automatico dell'alimentazione di un filo tubolare, in particolare in materiale prezioso nell'industria orafa
US4713740A (en) * 1984-07-27 1987-12-15 Sms Advanced Power, Inc. Switch-mode power supply
US4663292A (en) * 1984-12-21 1987-05-05 Wong Daniel T High-voltage biological macromolecule transfer and cell fusion system
US4946793A (en) * 1986-05-09 1990-08-07 Electropore, Inc. Impedance matching for instrumentation which electrically alters vesicle membranes
US4750100A (en) * 1986-06-06 1988-06-07 Bio-Rad Laboratories Transfection high voltage controller
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5389069A (en) * 1988-01-21 1995-02-14 Massachusetts Institute Of Technology Method and apparatus for in vivo electroporation of remote cells and tissue
US5186800A (en) * 1988-04-18 1993-02-16 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells
US4885943A (en) * 1988-05-11 1989-12-12 Hydro-Craft, Inc. Electronic flowmeter system and method
US5047007A (en) * 1989-12-22 1991-09-10 Medtronic, Inc. Method and apparatus for pulsed iontophoretic drug delivery
BE1004328A3 (fr) * 1990-05-16 1992-11-03 Scient Equipment Design & Dev Procede et dispositif de permeabilisation de cellules vivantes.
US5097403A (en) * 1991-02-11 1992-03-17 Astec International Ltd. Current sensing synchronous rectifier apparatus
US5169384A (en) * 1991-08-16 1992-12-08 Bosniak Stephen L Apparatus for facilitating post-traumatic, post-surgical, and/or post-inflammatory healing of tissue
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5501662A (en) * 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US5507724A (en) * 1992-07-01 1996-04-16 Genetronics, Inc. Electroporation and iontophoresis apparatus and method for insertion of drugs and genes into cells
US5304120A (en) * 1992-07-01 1994-04-19 Btx Inc. Electroporation method and apparatus for insertion of drugs and genes into endothelial cells
US5273525A (en) * 1992-08-13 1993-12-28 Btx Inc. Injection and electroporation apparatus for drug and gene delivery
US5315498A (en) * 1992-12-23 1994-05-24 International Business Machines Corporation Apparatus providing leading leg current sensing for control of full bridge power supply
US5439440A (en) * 1993-04-01 1995-08-08 Genetronics, Inc. Electroporation system with voltage control feedback for clinical applications
US5422272A (en) * 1993-07-14 1995-06-06 Andrew A. Papp Improvements to apparatus and method for electroporation
IL108775A (en) * 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
US5642035A (en) * 1994-06-16 1997-06-24 Bio-Rad Laboratories Transfection high-voltage controller
US5542967A (en) * 1994-10-06 1996-08-06 Ponizovsky; Lazar Z. High voltage electrical apparatus for removing ecologically noxious substances from gases
US5641680A (en) * 1994-11-14 1997-06-24 Zhao; Xi Gene transfer apparatus and method for using the same

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EP0925647B1 (de) 2002-04-10
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ES2175343T3 (es) 2002-11-16
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CA2261703A1 (en) 1998-03-12
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US5869326A (en) 1999-02-09
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