DE69627314D1 - Kettenabbruchgruppen, ihre verwendung zur sequenzierung und synthese von nukleinsäuren und verfahren zu ihrer herstellung - Google Patents

Kettenabbruchgruppen, ihre verwendung zur sequenzierung und synthese von nukleinsäuren und verfahren zu ihrer herstellung

Info

Publication number
DE69627314D1
DE69627314D1 DE69627314T DE69627314T DE69627314D1 DE 69627314 D1 DE69627314 D1 DE 69627314D1 DE 69627314 T DE69627314 T DE 69627314T DE 69627314 T DE69627314 T DE 69627314T DE 69627314 D1 DE69627314 D1 DE 69627314D1
Authority
DE
Germany
Prior art keywords
sequencing
synthesis
production
nucleic acids
chain termination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69627314T
Other languages
English (en)
Other versions
DE69627314T2 (de
Inventor
Marek Kwiatkowski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
QUIATECH UPPSALA AB
Original Assignee
QUIATECH UPPSALA AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by QUIATECH UPPSALA AB filed Critical QUIATECH UPPSALA AB
Application granted granted Critical
Publication of DE69627314D1 publication Critical patent/DE69627314D1/de
Publication of DE69627314T2 publication Critical patent/DE69627314T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE69627314T 1995-01-31 1996-01-30 Kettenabbruchgruppen, ihre verwendung zur sequenzierung und synthese von nukleinsäuren und verfahren zu ihrer herstellung Expired - Fee Related DE69627314T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9500342 1995-01-31
SE9500342A SE9500342D0 (sv) 1995-01-31 1995-01-31 Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
PCT/SE1996/000096 WO1996023807A1 (en) 1995-01-31 1996-01-30 Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation

Publications (2)

Publication Number Publication Date
DE69627314D1 true DE69627314D1 (de) 2003-05-15
DE69627314T2 DE69627314T2 (de) 2004-03-04

Family

ID=20397031

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69627314T Expired - Fee Related DE69627314T2 (de) 1995-01-31 1996-01-30 Kettenabbruchgruppen, ihre verwendung zur sequenzierung und synthese von nukleinsäuren und verfahren zu ihrer herstellung

Country Status (8)

Country Link
US (1) US6255475B1 (de)
EP (1) EP0808320B1 (de)
JP (1) JPH10513178A (de)
CA (1) CA2212000A1 (de)
DE (1) DE69627314T2 (de)
ES (1) ES2197231T3 (de)
SE (1) SE9500342D0 (de)
WO (1) WO1996023807A1 (de)

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US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
FR2790005B1 (fr) 1999-02-22 2004-01-30 Commissariat Energie Atomique Procede de fabrication de morpholino-nucleotides, et utilisation de ceux-ci pour l'analyse et le marquage de sequences d'acides nucleiques
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
EP1244782A2 (de) * 1999-12-23 2002-10-02 Axaron Bioscience AG Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20030228611A1 (en) * 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (de) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modifizierte nukleotide zur polynukleotidsequenzierung
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US7345163B2 (en) * 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
EP1725572B1 (de) 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
JP2008526877A (ja) * 2005-01-05 2008-07-24 エージェンコート パーソナル ジェノミクス 可逆的ヌクレオチドターミネーターおよびその使用
EP2241637A1 (de) 2005-02-01 2010-10-20 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
EP2239342A3 (de) 2005-02-01 2010-11-03 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
JP5430937B2 (ja) * 2005-10-27 2014-03-05 プレジデント アンド フェロウズ オブ ハーバード カレッジ 核酸を標識するための方法および組成物
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
US20070249014A1 (en) 2006-02-10 2007-10-25 Invitrogen Corporation Labeling and detection of post translationally modified proteins
BRPI0707479B1 (pt) * 2006-02-21 2018-06-05 Huntsman International Llc Processo para a produção de um compósito de poliisocianurato, e, compósito reativo
FI20065162A0 (fi) * 2006-03-13 2006-03-13 Wallac Oy Terminoivia DNA polymeraasisubstraatteja
US7482444B2 (en) * 2006-03-13 2009-01-27 Wallac Oy Terminating substrates for DNA polymerases
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US20090062132A1 (en) * 2007-08-29 2009-03-05 Borner Scott R Alternative nucleic acid sequencing methods
WO2009054922A1 (en) 2007-10-19 2009-04-30 The Trustees Of Columbia University In The City Of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A2 (de) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
EP2222341B1 (de) 2007-11-21 2015-02-25 University Of Georgia Research Foundation, Inc. Alkyne und verfahren zur reaktion von alkynen mit 1,3-dipol-funktionalen verbindungen
CN102119223A (zh) 2008-05-16 2011-07-06 生命技术公司 用于测量细胞增殖的双标记法
GB2473778B (en) * 2008-05-27 2013-04-10 Trilink Biotechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
US20100120034A1 (en) * 2008-07-03 2010-05-13 Life Technologies Corporation Methylation analysis of mate pairs
US9315860B2 (en) 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
DE102012008375A1 (de) 2011-04-27 2012-10-31 Genovoxx Gmbh Methoden und Komponenten zur Detektion von Nukleinsäureketten
US20150086981A1 (en) 2011-05-04 2015-03-26 Genovoxx Gmbh Nucleoside-triphosphate conjugate and methods for the use thereof
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
CN108864232B (zh) 2013-03-15 2022-01-04 伊鲁米纳剑桥有限公司 修饰的核苷或核苷酸
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
EP3699283A1 (de) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modifizierte vorlagenunabhängige enzyme zur polydeoxynukleotidsynthese
US11136565B2 (en) 2018-09-11 2021-10-05 Singular Genomics Systems, Inc. Modified archaeal family B polymerases
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
CA3165571C (en) 2019-12-23 2023-02-07 Singular Genomics Systems, Inc. Methods for long read sequencing
WO2021138094A1 (en) 2019-12-31 2021-07-08 Singular Genomics Systems, Inc. Polynucleotide barcodes for long read sequencing
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
WO2021178893A2 (en) 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
US20220145345A1 (en) 2020-11-11 2022-05-12 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping

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DE3739366A1 (de) 1987-04-10 1988-10-27 Boehringer Mannheim Gmbh Desaza-purin-nucleosid-derivate, verfahren zu deren herstellung sowie deren verwendung bei der nucleinsaeure-sequenzierung sowie als antivirale mittel
DE3916871A1 (de) 1989-05-24 1990-11-29 Boehringer Mannheim Gmbh Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
FR2703052B1 (fr) * 1993-03-26 1995-06-02 Pasteur Institut Nouvelle méthode de séquençage d'acides nucléiques.
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren

Also Published As

Publication number Publication date
US6255475B1 (en) 2001-07-03
DE69627314T2 (de) 2004-03-04
EP0808320A1 (de) 1997-11-26
CA2212000A1 (en) 1996-08-08
EP0808320B1 (de) 2003-04-09
WO1996023807A1 (en) 1996-08-08
ES2197231T3 (es) 2004-01-01
SE9500342D0 (sv) 1995-01-31
JPH10513178A (ja) 1998-12-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee