DE69603723D1 - Verfahren zum nachweis von nukleinsäuren - Google Patents

Verfahren zum nachweis von nukleinsäuren

Info

Publication number
DE69603723D1
DE69603723D1 DE69603723T DE69603723T DE69603723D1 DE 69603723 D1 DE69603723 D1 DE 69603723D1 DE 69603723 T DE69603723 T DE 69603723T DE 69603723 T DE69603723 T DE 69603723T DE 69603723 D1 DE69603723 D1 DE 69603723D1
Authority
DE
Germany
Prior art keywords
target
hybridized
nuclease
target sequence
heteroduplexes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69603723T
Other languages
English (en)
Other versions
DE69603723T2 (de
Inventor
Cassandra Smith
Ron Yaar
Przemyslaw Szafranski
Charles Cantor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Boston University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston University filed Critical Boston University
Application granted granted Critical
Publication of DE69603723D1 publication Critical patent/DE69603723D1/de
Publication of DE69603723T2 publication Critical patent/DE69603723T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
DE69603723T 1995-05-19 1996-05-20 Verfahren zum nachweis von nukleinsäuren Expired - Fee Related DE69603723T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/446,102 US5753439A (en) 1995-05-19 1995-05-19 Nucleic acid detection methods
PCT/US1996/006527 WO1996036731A2 (en) 1995-05-19 1996-05-20 Nucleic acid detection methods

Publications (2)

Publication Number Publication Date
DE69603723D1 true DE69603723D1 (de) 1999-09-16
DE69603723T2 DE69603723T2 (de) 2000-03-02

Family

ID=23771327

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69603723T Expired - Fee Related DE69603723T2 (de) 1995-05-19 1996-05-20 Verfahren zum nachweis von nukleinsäuren

Country Status (7)

Country Link
US (1) US5753439A (de)
EP (1) EP0827551B1 (de)
AT (1) ATE183244T1 (de)
AU (1) AU6248696A (de)
CA (1) CA2221467A1 (de)
DE (1) DE69603723T2 (de)
WO (1) WO1996036731A2 (de)

Families Citing this family (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
EP1262564A3 (de) 1993-01-07 2004-03-31 Sequenom, Inc. Dns-Sequenzierung durch Massenspektrometrie
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US6207373B1 (en) * 1998-02-25 2001-03-27 Nanogen, Inc. Methods for determining nature of repeat units in DNA
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US7803529B1 (en) * 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
DE19525632C2 (de) * 1995-07-14 1997-07-17 Bag Biolog Analysensystem Gmbh Sequenzspezifisches Nukleinsäurenachweisverfahren und Reagenz-System zu dessen Durchführung
US6146854A (en) * 1995-08-31 2000-11-14 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
JP2001517925A (ja) * 1995-12-22 2001-10-09 ベーリングウエルケ、アクティエンゲゼルシャフト 核酸の均質増幅および検出
JP3853845B2 (ja) * 1996-01-26 2006-12-06 アボツト・ラボラトリーズ リピート核酸配列を分析する方法
US5770370A (en) * 1996-06-14 1998-06-23 David Sarnoff Research Center, Inc. Nuclease protection assays
US5777324A (en) 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
WO1998023776A1 (en) * 1996-11-29 1998-06-04 Amersham Pharmacia Biotech Uk Ltd. Method for determining tandem repeat sequence length
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
WO1998029736A1 (en) * 1996-12-31 1998-07-09 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
ES2563643T3 (es) * 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
JP4185185B2 (ja) * 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖ｄｎａを利用したｄｎａの検出方法
US20030054378A1 (en) * 1997-05-08 2003-03-20 Isao Karube Method for detecting target nucleotide sequence
WO1998053098A1 (de) * 1997-05-21 1998-11-26 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Verfahren und kit zur detektion von mutationen in dna's mit hilfe von restriktionsenzymen
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
ATE225858T1 (de) * 1997-07-28 2002-10-15 Medical Biosystems Ltd Sequenzanalyse von nukleinsäuren
EP1041160A4 (de) * 1997-07-31 2003-05-28 Rikagaku Kenkyusho Verfahren zur detektion einer mutation innerhalb einer basensequenz
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
EP1021558A1 (de) * 1997-10-10 2000-07-26 President And Fellows Of Harvard College Oberflächengebundene, doppelsträngige dna-protein-arrays
AU737174B2 (en) 1997-10-10 2001-08-09 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
WO1999028494A1 (en) * 1997-12-04 1999-06-10 Packard Bioscience Company Methods of using probes for analyzing polynucleotide sequence
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US20030039967A1 (en) * 1997-12-19 2003-02-27 Kris Richard M. High throughput assay system using mass spectrometry
US20030096232A1 (en) * 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US6087102A (en) * 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
WO1999036571A2 (en) * 1998-01-13 1999-07-22 Biochip Technologies Gmbh Method for the detection or nucleic acid of nucleic acid sequences
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AR021833A1 (es) * 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2000028090A2 (en) 1998-11-12 2000-05-18 Nyxis, Inc. Diagnostic assay for cancer
US6287773B1 (en) * 1999-05-19 2001-09-11 Hoeschst-Ariad Genomics Center Profile searching in nucleic acid sequences using the fast fourier transformation
CA2377739A1 (en) 1999-07-02 2001-01-11 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
US6342362B1 (en) * 1999-08-05 2002-01-29 Daniel S. Mytelka Methods and compositions for the purification of proteins or other macromolecules
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
US7875442B2 (en) * 2000-03-24 2011-01-25 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
US7829313B2 (en) * 2000-03-24 2010-11-09 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
JP4589480B2 (ja) * 2000-03-30 2010-12-01 オリンパス株式会社 反復配列の多型を分析する方法
EP1158058A1 (de) * 2000-05-19 2001-11-28 Centre National De La Recherche Scientifique Zusammensetzungen und Verfahren geeignet für Nukleinsäureanalyse
US6984522B2 (en) 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
AU2001285219A1 (en) * 2000-08-24 2002-03-04 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
US20040185464A1 (en) * 2000-09-15 2004-09-23 Kris Richard M. High throughput assay system
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
AU2002246978A1 (en) * 2001-01-10 2002-07-24 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface
WO2002061116A1 (en) * 2001-01-31 2002-08-08 The General Hospital Corporation In vivo screening array
EP1430150B1 (de) 2001-09-24 2015-08-19 One Lambda, Inc. Diagnostisches sondennachweissystem
AU2003213107A1 (en) 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
US6713262B2 (en) * 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
CA2507189C (en) * 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
US20050009053A1 (en) * 2003-04-25 2005-01-13 Sebastian Boecker Fragmentation-based methods and systems for de novo sequencing
US9394565B2 (en) * 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US7964344B2 (en) * 2003-09-17 2011-06-21 Canon Kabushiki Kaisha Stable hybrid
JP4339062B2 (ja) * 2003-09-30 2009-10-07 富士フイルム株式会社 ミスマッチ領域検出方法
US7662555B2 (en) 2003-12-24 2010-02-16 Shinichiro Isobe Method for detecting biomolecule, labeling dye used therefore, and labeling kit
US20050191636A1 (en) * 2004-03-01 2005-09-01 Biocept, Inc. Detection of STRP, such as fragile X syndrome
WO2005098050A2 (en) * 2004-03-26 2005-10-20 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US20080241827A1 (en) * 2004-05-10 2008-10-02 Exact Sciences Corporation Methods For Detecting A Mutant Nucleic Acid
US20060008823A1 (en) * 2004-05-12 2006-01-12 Kemp Jennifer T DNA profiling and SNP detection utilizing microarrays
NZ551996A (en) * 2004-05-13 2010-07-30 Anita Goel Nano-PCR: Methods and devices for nucleic acid amplification and detection
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US20060073501A1 (en) * 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
US7238486B2 (en) * 2004-09-21 2007-07-03 The Board Of Trustees Of The Leland Stanford Junior University DNA fingerprinting using a branch migration assay
US7501253B2 (en) * 2004-09-21 2009-03-10 The Board Of Trustees Of The Leland Stanford Junior University DNA fingerprinting using a branch migration assay
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
WO2007044071A2 (en) * 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) * 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US20080318215A1 (en) * 2005-12-20 2008-12-25 Ming-Sheng Lee Apparatus, methods and products for detecting genetic mutation
EP2021503A1 (de) * 2006-03-17 2009-02-11 Solexa Ltd. Isothermische methoden zur entstehung von arrays von einzelnen molekülen
US9862984B2 (en) * 2006-04-21 2018-01-09 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
US20080076121A1 (en) * 2006-09-22 2008-03-27 Paul Kenneth Wolber Microarray nuclease protection assay
TWI412593B (zh) * 2006-12-20 2013-10-21 Ming-Sheng Lee 基因變異檢體的檢測方法及用具組
US7888069B2 (en) * 2006-12-22 2011-02-15 Dow Agrosciences Llc Plant-made west nile virus (WNV) vaccines, vectors and plant codon optimized sequences
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US7947446B2 (en) * 2007-05-29 2011-05-24 Ming-Sheng Lee High throughput mutation screening methods and kits using a universalized approach—differential sequence fill-in (DSF)-enabled sequential adapter ligation and amplification
WO2009039122A2 (en) 2007-09-17 2009-03-26 Sequenom, Inc. Integrated robotic sample transfer device
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US20130183666A1 (en) 2012-01-18 2013-07-18 Marc N. Feiglin Partial genotyping by differential hybridization
US20150050649A1 (en) * 2012-03-22 2015-02-19 Wako Pure Chemical Industries, Ltd. Method for detecting dna having microsatellite region
AU2013282319B2 (en) 2012-06-29 2018-05-17 Htg Molecular Diagnostics, Inc. Nuclease protection methods for detection of nucleotide variants
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
GB2563357A (en) * 2016-02-10 2018-12-12 Nec Corp DNA testing chip, DNA testing method, DNA testing system, and DNA testing chip control device

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6834187A (en) * 1985-12-17 1987-07-15 Genetics Institute Inc. Displacement polynucleotide method and reagent complex
US4946773A (en) * 1985-12-23 1990-08-07 President And Fellows Of Harvard College Detection of base pair mismatches using RNAase A
GB8606719D0 (en) * 1986-03-19 1986-04-23 Lister Preventive Med Genetic probes
CA1341576C (en) * 1986-08-11 2008-07-08 Thaddeus P. Dryja Diagnosis of retinoblastoma
US5032502A (en) * 1988-01-21 1991-07-16 The United States Of America As Represented By The United States Of Energy Purification of polymorphic components of complex genomes
US5459039A (en) * 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
WO1991015600A1 (en) * 1990-03-30 1991-10-17 City Of Hope Detection of minimal residual disease in lymphoid malignancies
DE69132843T2 (de) * 1990-12-06 2002-09-12 Affymetrix Inc N D Ges D Staat Identifizierung von Nukleinsäuren in Proben
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
US5695933A (en) * 1993-05-28 1997-12-09 Massachusetts Institute Of Technology Direct detection of expanded nucleotide repeats in the human genome
WO1995021269A1 (en) * 1994-02-04 1995-08-10 Perlin Mark K Method and apparatus for analyzing genetic material

Also Published As

Publication number Publication date
ATE183244T1 (de) 1999-08-15
EP0827551A2 (de) 1998-03-11
EP0827551B1 (de) 1999-08-11
WO1996036731A2 (en) 1996-11-21
AU6248696A (en) 1996-11-29
CA2221467A1 (en) 1996-11-21
US5753439A (en) 1998-05-19
DE69603723T2 (de) 2000-03-02
WO1996036731A3 (en) 1997-02-06

Similar Documents

Publication Publication Date Title
ATE183244T1 (de) Verfahren zum nachweis von nukleinsäuren
US6258539B1 (en) Restriction enzyme mediated adapter
CA2325468C (en) Rolling circle replication of padlock probes
JP3863189B2 (ja) Ｄｎａへの特徴付け
US6858413B2 (en) Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
EP0481704B1 (de) Amplifikation von Midivariant-DNS-Templaten
US20050164205A1 (en) Charge and mass tags for detection and analysis
US8211644B2 (en) Molecular beacon-based methods for detection of targets using abscription
ATE188744T1 (de) Verfahren zur erhöhung der nachweisempfindlichkeit von nukleinsäure-hybriden (target/probe)
BR9004881A (pt) Processo para detectar uma sequencia de acido nucleico,processo para determinar quantitativamente a extensao de hibridizacao,ensaio de hibridizacao,estojo,bases,sondas,processo para detectar chlamydia trachomatis e lamina de microtitulacao
JP2005537030A5 (de)
DE69333242D1 (de) Verfahren, reagenz und salz zum nachweis und amplifikation von nukleinsäuresequenzen
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
ATE339514T1 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
DE69637719D1 (de) Markierte zwei-konformations oligonukleotid Sonden, Verfahren und Testsätze
ATE388402T1 (de) Verfahren zur erkennung und zum assay von nukleinsäuresequenzen
WO1999010540A1 (en) Dna methyltransferase genotyping
DE60335116D1 (de) Verfahren zum nachweis von sequenzunterschieden
JP3752466B2 (ja) 遺伝子検査方法
ATE398186T1 (de) Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren
ATE211507T1 (de) Nachweis von nukleotidsequenzen durch signalamplifizierung
WO1999028494A1 (en) Methods of using probes for analyzing polynucleotide sequence
Li et al. CE combined with rolling circle amplification for sensitive DNA detection
Wagner et al. Mismatch binding protein-based mutation detection systems
Lavery et al. Analysis of differential gene expression using the SABRE enrichment protocol

Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee