DE69531660D1 - Klonierte dna polymerasen aus thermotoga neapolitana und resultierende mutanten - Google Patents

Klonierte dna polymerasen aus thermotoga neapolitana und resultierende mutanten

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Publication number
DE69531660D1
DE69531660D1 DE69531660T DE69531660T DE69531660D1 DE 69531660 D1 DE69531660 D1 DE 69531660D1 DE 69531660 T DE69531660 T DE 69531660T DE 69531660 T DE69531660 T DE 69531660T DE 69531660 D1 DE69531660 D1 DE 69531660D1
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DE
Germany
Prior art keywords
dna polymerase
tne
mutation
thermotoga neapolitana
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE69531660T
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English (en)
Inventor
A John Hughes Jr
Deb K Chatterjee
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Life Technologies Corp
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Invitrogen Corp
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Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
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Publication of DE69531660D1 publication Critical patent/DE69531660D1/de
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Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
DE69531660T 1994-09-30 1995-10-02 Klonierte dna polymerasen aus thermotoga neapolitana und resultierende mutanten Expired - Lifetime DE69531660D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31642394A 1994-09-30 1994-09-30
US08/370,190 US5912155A (en) 1994-09-30 1995-01-09 Cloned DNA polymerases from Thermotoga neapolitana
PCT/US1995/012358 WO1996010640A1 (en) 1994-09-30 1995-10-02 Cloned dna polymerases from thermotoga neapolitana and mutants thereof

Publications (1)

Publication Number Publication Date
DE69531660D1 true DE69531660D1 (de) 2003-10-09

Family

ID=26980432

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69531660T Expired - Lifetime DE69531660D1 (de) 1994-09-30 1995-10-02 Klonierte dna polymerasen aus thermotoga neapolitana und resultierende mutanten

Country Status (7)

Country Link
US (4) US5912155A (de)
EP (2) EP0725827B1 (de)
JP (1) JPH09506783A (de)
AT (1) ATE248917T1 (de)
CA (1) CA2174944A1 (de)
DE (1) DE69531660D1 (de)
WO (1) WO1996010640A1 (de)

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EP0725827B1 (de) 2003-09-03
US20030092018A1 (en) 2003-05-15
EP0725827A1 (de) 1996-08-14
WO1996010640A1 (en) 1996-04-11
US5939301A (en) 1999-08-17
EP1361275A1 (de) 2003-11-12
ATE248917T1 (de) 2003-09-15
US5912155A (en) 1999-06-15
US6444424B1 (en) 2002-09-03
CA2174944A1 (en) 1996-04-11
JPH09506783A (ja) 1997-07-08

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