DE69521219T2 - Modifizierte grün fluorszierende proteine - Google Patents

Modifizierte grün fluorszierende proteine

Info

Publication number
DE69521219T2
DE69521219T2 DE69521219T DE69521219T DE69521219T2 DE 69521219 T2 DE69521219 T2 DE 69521219T2 DE 69521219 T DE69521219 T DE 69521219T DE 69521219 T DE69521219 T DE 69521219T DE 69521219 T2 DE69521219 T2 DE 69521219T2
Authority
DE
Germany
Prior art keywords
wild
gfp
product derived
modifications
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69521219T
Other languages
English (en)
Other versions
DE69521219D1 (de
Inventor
Y Tsien
Roger Heim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23322562&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69521219(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of California filed Critical University of California
Publication of DE69521219D1 publication Critical patent/DE69521219D1/de
Application granted granted Critical
Publication of DE69521219T2 publication Critical patent/DE69521219T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/855—Proteins from animals other than mammals or birds
DE69521219T 1994-11-10 1995-11-13 Modifizierte grün fluorszierende proteine Expired - Lifetime DE69521219T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/337,915 US5625048A (en) 1994-11-10 1994-11-10 Modified green fluorescent proteins
PCT/US1995/014692 WO1996023810A1 (en) 1994-11-10 1995-11-13 Modified green fluorescenct proteins

Publications (2)

Publication Number Publication Date
DE69521219D1 DE69521219D1 (de) 2001-07-12
DE69521219T2 true DE69521219T2 (de) 2001-10-25

Family

ID=23322562

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69521219T Expired - Lifetime DE69521219T2 (de) 1994-11-10 1995-11-13 Modifizierte grün fluorszierende proteine

Country Status (12)

Country Link
US (4) US5625048A (de)
EP (2) EP0804457B1 (de)
JP (1) JP3283523B2 (de)
AT (1) ATE201878T1 (de)
AU (1) AU702205B2 (de)
CA (1) CA2205006C (de)
DE (1) DE69521219T2 (de)
DK (2) DK0804457T3 (de)
ES (1) ES2159650T3 (de)
GR (1) GR3036264T3 (de)
PT (1) PT804457E (de)
WO (1) WO1996023810A1 (de)

Families Citing this family (359)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
CA2232727C (en) * 1995-09-22 2002-03-26 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6020192A (en) * 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
US6900304B2 (en) 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5804387A (en) * 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6027881A (en) 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US5912137A (en) * 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
AU767375B2 (en) * 1996-08-16 2003-11-06 Aurora Biosciences Corporation Long wavelength engineered fluorescent proteins
US6124128A (en) * 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
WO1998021355A1 (en) * 1996-11-15 1998-05-22 Life Technologies, Inc. Mutants of green fluorescent protein
IL129767A0 (en) 1996-12-12 2000-02-29 Prolume Ltd Apparatus and method for detecting and identifying infectious agents
US6090919A (en) * 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5994077A (en) * 1997-01-31 1999-11-30 The Board Of Trustees Of The Leland Stanford Junior University Flourescence-based isolation of differentially induced genes
EP0981633A2 (de) * 1997-02-13 2000-03-01 Memorial Sloan-Kettering Cancer Center Hybrid-moleküle zur optischen detektion von veränderungen in zellulären mikro-umgebungen
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
DE19715683C2 (de) * 1997-04-15 1999-11-11 Max Planck Gesellschaft Neue Reportervektoren für One-Hybrid und Two-Hybrid Systeme
US6528271B1 (en) * 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US5891646A (en) * 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US7541151B2 (en) 1997-06-05 2009-06-02 Duke University Single-cell biosensor for the measurement of GPCR ligands in a test sample
FR2764387B1 (fr) 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US6221612B1 (en) * 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6130313A (en) * 1997-10-02 2000-10-10 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins
US6265174B1 (en) 1997-11-03 2001-07-24 Morphochem, Inc. Methods and compositions for identifying and modulating ctionprotein-interactions
US6734338B1 (en) 1997-11-14 2004-05-11 Cedars-Sinai Medical Center Transfection, storage and transfer of male germ cells for generation of transgenic species and genetic therapies
US7294755B1 (en) 1997-11-14 2007-11-13 Cedars-Sinai Medical Center Genetic modification of male germ cells for generation of transgenic species and genetic therapies
ATE426036T1 (de) * 1997-11-14 2009-04-15 Cedars Sinai Medical Center Transfektion und transfer nicht humaner mannlicher keimzellen zur generierung transgener nicht humaner saugetiere
US6194548B1 (en) * 1998-01-23 2001-02-27 Takashi Osumi Green fluorescent proteins and blue fluorescent proteins
WO1999046283A1 (en) * 1998-03-09 1999-09-16 Zealand Pharmaceuticals A/S Pharmacologically active peptide conjugates having a reduced tendency towards enzymatic hydrolysis
US6080576A (en) * 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
US6808921B1 (en) 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6232107B1 (en) 1998-03-27 2001-05-15 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
EP0949269A1 (de) * 1998-04-02 1999-10-13 SymBiosis GmbH Biosensor Protein
US6197947B1 (en) 1998-06-01 2001-03-06 The Rockefeller University Translation initiation factor 4AIII and methods of use thereof
US6140132A (en) * 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6150176A (en) 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6495664B1 (en) 1998-07-24 2002-12-17 Aurora Biosciences Corporation Fluorescent protein sensors of post-translational modifications
WO2000008054A1 (en) * 1998-08-08 2000-02-17 Imperial Cancer Research Technology Limited Modified green fluorescent protein
US7297482B2 (en) * 1998-10-08 2007-11-20 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
US7338782B2 (en) 1998-12-11 2008-03-04 Clontech Laboratories, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US9834584B2 (en) * 1998-12-11 2017-12-05 Takara Bio Usa, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US20030087492A1 (en) 2001-11-02 2003-05-08 Promos Technologies, Inc. Semiconductor device and method of manufacturing the same
US20020103359A1 (en) * 1999-12-07 2002-08-01 Gregory Donoho Novel human membrane proteins and polynucleotides encoding the same
US20020031802A1 (en) * 2000-05-12 2002-03-14 Yi Hu Novel seven transmembrane proteins and polynucleotides encoding the same
US20020061556A1 (en) * 2000-04-27 2002-05-23 Walke D. Wade Novel membrane proteins and polynucleotides encoding the same
US20020055626A1 (en) * 2000-06-09 2002-05-09 Turner C. Alexander Novel human seven transmembrane proteins and polynucleotides encoding the same
US20060257884A1 (en) * 2004-05-20 2006-11-16 Amnis Corporation Methods for preparing and analyzing cells having chromosomal abnormalities
US7450229B2 (en) * 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US8131053B2 (en) * 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8406498B2 (en) * 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
JP2002536009A (ja) * 1999-02-09 2002-10-29 レキシコン・ジェネティクス・インコーポレーテッド ヒト脱共役タンパク質およびそれをコードするポリヌクレオチド
US6670144B1 (en) 1999-02-26 2003-12-30 Cyclacel, Ltd. Compositions and methods for monitoring the phosphorylation of natural binding partners
US8148110B2 (en) 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
DE19915137C2 (de) * 1999-03-26 2001-10-18 Michael Schaefer Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
WO2001009177A2 (en) * 1999-07-29 2001-02-08 Whitehead Institute For Biomedical Research Affinity fluorescent proteins and uses thereof
NL1012782C2 (nl) * 1999-08-05 2001-02-06 Keygene Nv Verbeterde gfp-variant, bruikbaar als markering, en gfp-construct.
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6716614B1 (en) * 1999-09-02 2004-04-06 Lexicon Genetics Incorporated Human calcium dependent proteases, polynucleotides encoding the same, and uses thereof
US6861220B2 (en) 1999-09-08 2005-03-01 Ramot University Authority For Applied Research & Industrial Development Ltd Genetic screening methods
WO2001018206A1 (en) * 1999-09-08 2001-03-15 Lexicon Genetics Incorporated Human g-protein coupled 7 transmembrane receptor and polynucleotides encoding the same
US6541252B1 (en) 2000-05-19 2003-04-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
EP1222269A1 (de) 1999-10-12 2002-07-17 Lexicon Genetics Incorporated Menschliche ldl rezeptorfamilieproteine und polynukleinesäuren die dafür kodieren
AU1198701A (en) * 1999-10-13 2001-04-23 Lexicon Genetics Incorporated Novel human membrane proteins
JP2003527833A (ja) 1999-10-14 2003-09-24 クロンテック・ラボラトリーズ・インコーポレーテッド 花虫類に由来する発色団／蛍光体、およびそれらの使用法
EP1226170B1 (de) * 1999-10-18 2008-07-30 Lexicon Pharmaceuticals, Inc. Menschliches chordin und polynukleotiden die dafür kodieren
WO2001051634A1 (en) * 2000-01-12 2001-07-19 Lexicon Genetics Incorporated Human olfactory receptor and polynucleotides encoding the same
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
AU782817B2 (en) * 2000-02-04 2005-09-01 Lexicon Pharmaceuticals, Inc. Novel human G protein coupled receptor proteins and polynucleotides encoding the same
JP4342069B2 (ja) * 2000-02-14 2009-10-14 生化学工業株式会社 α１，４−ガラクトース転移酵素およびそれをコードするＤＮＡ
WO2001062919A1 (en) * 2000-02-23 2001-08-30 Aurora Biosciences Corporation Modified fluorescent proteins
US20020064842A1 (en) * 2000-06-09 2002-05-30 Sorge Joseph A. Renilla reniformis green fluorescent protein and mutants thereof
US7029878B2 (en) * 2000-03-15 2006-04-18 Merck & Co., Inc. Melanin concentrating hormone receptor chimeric and fusion proteins
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US6686188B2 (en) * 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
US6656700B2 (en) * 2000-05-26 2003-12-02 Amersham Plc Isoforms of human pregnancy-associated protein-E
US6790667B1 (en) * 2000-05-30 2004-09-14 Lexicon Genetics Incorporated Human mitochondrial proteins and polynucleotides encoding the same
DK1299414T3 (da) * 2000-06-19 2009-11-30 Fisher Bioimage Aps Fluorescerende proteiner
US6667153B1 (en) 2000-06-26 2003-12-23 Susan Margaret Thomas Composition and method for detecting mutagens
US6806289B1 (en) 2000-07-14 2004-10-19 Stephen J. Lippard Coordination complexes, and methods for preparing by combinatorial methods, assaying and using the same
US20020123474A1 (en) * 2000-10-04 2002-09-05 Shannon Mark E. Human GTP-Rho binding protein2
US7009651B2 (en) 2000-10-12 2006-03-07 Amnis Corporation System and method for high numeric aperture imaging systems
AU2002211722B2 (en) * 2000-10-12 2007-03-15 Clontech Laboratories, Inc. Nucleic acids encoding stichodactylidae chromoproteins
EP1409674A2 (de) * 2000-10-27 2004-04-21 Lexicon Genetics Incorporated Humane 7tm proteine und dafür kodierende polynukleotide
JP2004531209A (ja) * 2000-10-30 2004-10-14 レキシコン・ジェネティクス・インコーポレーテッド 新規ヒト７ｔｍタンパクおよびそれをコードするポリヌクレオチド
US6977293B1 (en) 2000-11-03 2005-12-20 Ceres, Inc. Chimeric polypeptides
US7018812B2 (en) * 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
US20050136431A1 (en) * 2000-11-03 2005-06-23 Oakley Robert H. Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7163800B2 (en) * 2000-11-03 2007-01-16 Molecular Devices Corporation Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7279324B2 (en) * 2001-01-23 2007-10-09 Duke University Nucleic acid encoding G-protein coupled receptor with modified DRY motif
US20020132318A1 (en) * 2001-01-29 2002-09-19 Ming Zhao Fluorescent proteins
US6969597B2 (en) 2001-02-21 2005-11-29 Clontech Laboratories, Inc. Nucleic acids encoding non aggregating fluorescent proteins and methods for using the same
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
WO2002068605A2 (en) * 2001-02-26 2002-09-06 The Regents Of The University Of California Non-oligomerizing tandem fluorescent proteins
US7687614B2 (en) * 2001-02-26 2010-03-30 The Regents Of The University Of California Monomeric and dimeric fluorescent protein variants and methods for making same
JP3929250B2 (ja) * 2001-03-08 2007-06-13 株式会社ルネサステクノロジ 半導体装置
NZ528000A (en) * 2001-03-09 2005-09-30 Gene Stream Pty Ltd A construct comprising in operable linkage a polynucleotide that encodes a polypeptide with a half-life of less than three hours
WO2002077011A2 (en) * 2001-03-12 2002-10-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation-reduction sensitive green fluorescent protein variants
GB0109858D0 (en) 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
US20030092884A1 (en) * 2001-05-25 2003-05-15 Lukyanov Sergey A. Kindling fluorescent proteins and methods for their use
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
EP1407005A4 (de) * 2001-06-11 2006-05-31 Interleukin Genetics Inc Integrative assays zur überwachung molekularer zusammenbauereignisse
WO2003008435A2 (en) * 2001-07-06 2003-01-30 Board Of Trustees Of The University Of Illinois Gfp fusion proteins and their use
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
US20040096880A1 (en) * 2001-08-07 2004-05-20 Kmiec Eric B. Compositions and methods for the treatment of diseases exhibiting protein misassembly and aggregation
CA2455424A1 (en) * 2001-08-07 2003-02-20 University Of Delaware Compositions and methods for the prevention and treatment of huntington's disease
US6900289B2 (en) * 2001-08-22 2005-05-31 The University Of Hawaii Physalia fluorescent proteins
CA2459161A1 (en) 2001-08-31 2003-03-13 The Rockefeller University Phosphodiesterase activity and regulation of phosphodiesterase 1b-mediated signaling in brain
CA2461824A1 (en) * 2001-10-12 2003-04-17 Clontech Laboratories, Inc. Nucleic acids encoding linked chromo/fluorescent domains and methods for using the same
AU2002363466A1 (en) * 2001-10-24 2003-05-19 University Of Hawaii Multifunctional green fluorescent protein and uses thereof
DE10153829A1 (de) * 2001-11-05 2003-05-28 Bayer Ag Assay basierend auf dotierten Nanoteilchen
WO2003042401A2 (en) * 2001-11-13 2003-05-22 Clontech Laboratories, Inc. Novel chromophores/fluorophores and methods for using the same
US20030104439A1 (en) * 2001-11-30 2003-06-05 Finch Rosalynde J. Methods of identifying cellular target molecules
CA2467383C (en) * 2001-12-19 2012-08-28 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
JP2005514032A (ja) * 2001-12-26 2005-05-19 クローンテック ラボラトリーズ インク． 変異体発色団／蛍光団ならびにそれらの製造法および使用法
AU2003208520B2 (en) * 2002-01-22 2008-06-05 Takara Bio Usa, Inc. Fluorescent protein from aequorea coerulscens and uses therof
US6918839B2 (en) * 2002-01-28 2005-07-19 The Boeing Company Damage tolerant shaft
WO2003068357A2 (en) * 2002-02-12 2003-08-21 Cornell Research Foundation, Inc. Identification and high-yield isolation of human pancreatic islet progenitor and stem cells
KR101076102B1 (ko) 2002-02-21 2011-10-21 비로노바티브 비.브이. 메타뉴모바이러스에서 유래한 이종성 항원을 포함하는재조합 파라인플루엔자 바이러스 발현 시스템 및 백신
KR100468225B1 (ko) * 2002-03-08 2005-01-26 (주)뉴로제넥스 형광강도가 증강된 삽입형 녹색 형광 단백질 및 그유전자
US20030170611A1 (en) * 2002-03-09 2003-09-11 Cardone Michael H. Cell-based screening methods
US20030170850A1 (en) * 2002-03-09 2003-09-11 Cardone Michael H. Cell-based screening methods
US6730492B2 (en) * 2002-03-09 2004-05-04 Merrimack Pharmaceuticals, Inc. Cell-based screening methods
US20030182669A1 (en) * 2002-03-19 2003-09-25 Rockman Howard A. Phosphoinositide 3-kinase mediated inhibition of GPCRs
KR20030076735A (ko) * 2002-03-21 2003-09-29 김경진 변종삽입형광단백질
GB0207533D0 (en) 2002-04-02 2002-05-08 Oxford Glycosciences Uk Ltd Protein
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
US7666587B2 (en) * 2002-04-12 2010-02-23 New York University Method of screening test substances for treating or preventing a disease mediated by plasma cells
US7271241B2 (en) * 2002-04-24 2007-09-18 Los Alamos National Security, Llc Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby
DE60325719D1 (de) * 2002-05-13 2009-02-26 Molecular Devices Corp Konstitutiv translozierende zelllinie
FR2851379B1 (fr) * 2003-02-18 2008-02-01 Cit Alcatel Convertisseur en transfert direct d'energie
US7351542B2 (en) 2002-05-20 2008-04-01 The Regents Of The University Of California Methods of modulating tubulin deacetylase activity
US7290215B2 (en) * 2002-06-03 2007-10-30 Microsoft Corporation Dynamic wizard interface system and method
EP3170890A1 (de) 2002-08-21 2017-05-24 Revivicor, Inc. Schweine ohne expression von funktionaler alpha-1,3-galactosyltransferase
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2504443A1 (en) * 2002-10-30 2004-05-13 Pointilliste, Inc. Methods for producing polypeptide-tagged collections and capture systems containing the tagged polypeptides
AU2003285844A1 (en) 2002-11-12 2004-06-03 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same
US7056683B2 (en) * 2002-11-12 2006-06-06 Massachusetts Institute Of Technology Genetically encoded fluorescent reporters of kinase, methyltransferase, and acetyl-transferase activities
WO2004046340A2 (en) * 2002-11-14 2004-06-03 Genentech, Inc. Intron fusion construct and method of using for selecting high-expressing production cell lines
US7678893B2 (en) 2002-12-26 2010-03-16 Zakrytoe Aktsionernoe Obschestvo “Evrogen” Fluorescent proteins from Copepoda species and methods for using same
EP1590438A2 (de) * 2003-01-24 2005-11-02 Duke University Modifizierte transportmuster für arrestin und g-protein-gekoppelte rezeptoren über arrestin-ubiquitin-chimäre
US20040241748A1 (en) * 2003-02-10 2004-12-02 Dana Ault-Riche Self-assembling arrays and uses thereof
US20070178456A1 (en) 2003-03-27 2007-08-02 Trotta Christopher R Targeting enzymes of the trna splicing pathway for identification of anti-fungal and/or anti-proliferative molecules
EP2319318A3 (de) 2003-03-27 2011-08-17 PTC Therapeutics, Inc. Verfahren zur Identifizierung von Verbindungen mit tRNA-Splicing Endonuklease als Aufhänger, und Verwendungen für diese Verbindungen als anti-Proliferationsmittel
US7083931B2 (en) * 2003-04-04 2006-08-01 Stratagene California Renilla GFP mutants with increased fluorescent intensity and spectral shift
US7160698B2 (en) * 2003-05-22 2007-01-09 University Of Florida Research Foundation, Inc. Fluorescent and colored proteins, and polynucleotides that encode these proteins
CA2528120A1 (en) 2003-06-10 2005-01-20 The J. David Gladstone Institutes Methods for treating lentivirus infections
US7619059B2 (en) * 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (en) * 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US8105798B2 (en) * 2003-08-13 2012-01-31 The Burnham Institute Cytoplasmic activity of retinoid X receptor and its regulation by ligands and dimerization
US20050037364A1 (en) * 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
JP2007505315A (ja) * 2003-09-12 2007-03-08 インヴィトロジェン コーポレーション 多重結合および活性アッセイ
JP2007507224A (ja) * 2003-09-30 2007-03-29 レキシコン ジェネティックス インコーポレーテッド 遺伝子の機能を定義するための方法および組成物
EP1692156B1 (de) 2003-10-24 2012-12-26 Los Alamos National Security, LLC Sich selbst zusammenbauende systeme getrennter fluoreszierender proteine
US20050095648A1 (en) * 2003-10-30 2005-05-05 Mario Geysen Method for designing linear epitopes and algorithm therefor and polypeptide epitopes
US20050287668A1 (en) * 2003-11-04 2005-12-29 Cell Therapeutics, Inc. (Cti) RNA interference compositions and screening methods for the identification of novel genes and biological pathways
CA2549031A1 (en) * 2003-12-10 2005-06-30 University Of Utah Research Foundation Method for obtaining an enriched population of sirna-expressing cells
US20060018911A1 (en) * 2004-01-12 2006-01-26 Dana Ault-Riche Design of therapeutics and therapeutics
WO2005081783A2 (en) 2004-01-30 2005-09-09 Dana Farber Cancer Institute Method for determination and quantification of radiation or genotoxin exposure
ATE538138T1 (de) 2004-03-16 2012-01-15 Amnis Corp Auf bildlicher darstellung beruhende quantifizierung molekularer translokation
US8103080B2 (en) 2004-03-16 2012-01-24 Amnis Corporation Method for imaging and differential analysis of cells
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells
EP1732944B1 (de) 2004-04-07 2012-09-05 The University of Chicago Monomere, rotfluoreszierende proteine
US7504205B2 (en) * 2004-05-17 2009-03-17 The Burnham Institute Uncharacterized ORF3 in SARS-coronavirus is a cyclic-AMP-dependent kinase and a target for SARS therapy
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
US20110039254A1 (en) 2004-09-14 2011-02-17 Pinheiro Leonardo B Chromosomal Insertion of Gfp Into Bacteria For Quality Control
WO2006044716A2 (en) 2004-10-15 2006-04-27 Washington University In St.Louis CELL PERMEABLE NANOCONJUGATES OF SHELL-CROSSLINKED KNEDEL (SCK) AND PEPTIDE NUCLEIC ACIDS ('PNAs') WITH UNIQUELY EXPRESSED OR OVER-EXPRESSED mRNA TARGETING SEQUENCES FOR EARLY DIAGNOSIS AND THERAPY OF CANCER
US7709208B2 (en) * 2004-11-08 2010-05-04 New York University Methods for diagnosis of major depressive disorder
US7393923B2 (en) 2004-11-08 2008-07-01 The Regents Of The University Of California Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same
US20090263821A1 (en) * 2004-12-01 2009-10-22 Proteologics, Inc. Ubiquitin Ligase Assays And Related Reagents
US7230080B2 (en) * 2005-02-16 2007-06-12 University Of Florida Research Foundation, Inc. Fluorescent and colored proteins, and polynucleotides that encode these proteins
AU2006340711C1 (en) 2005-04-05 2013-02-07 Allergan, Inc. Clostridial toxin activity assays
EP2949668B1 (de) 2005-05-18 2019-08-14 Ablynx N.V. Verbesserte nanokörper-tm gegen tumornekrosefaktor alpha
RU2433139C2 (ru) 2005-05-20 2011-11-10 Аблинкс Н.В. Nanobodies tm для лечения заболеваний, опосредованных агрегацией
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
EP1901659B1 (de) 2005-07-13 2018-04-25 Georgia State University Research Foundation, Inc. Kontrastmittel und verfahren zur herstellung von kontrastmitteln
EP1924686A4 (de) 2005-08-09 2009-11-11 Revivicor Inc Ctla4-ig exprimierende transgene huftiere und deren verwendung
JP5377965B2 (ja) 2005-09-09 2013-12-25 ジョージア ステート ユニバーシティ リサーチ ファウンデーション、インコーポレイテッド 標的化された造影剤および造影剤を標的化するための方法
US20100009348A1 (en) * 2005-09-28 2010-01-14 Attagene, Inc. Methods and Constructs for Analyzing Biological Activities of Biological Specimens and Determining States of Organism
US20090149338A1 (en) * 2005-09-30 2009-06-11 Hughes Thomas E System for detecting protein-protein interactions
WO2007052102A2 (en) * 2005-11-04 2007-05-10 Evrogen, Jsc Modified green fluorescent proteins and methods for using same
EP1785434A1 (de) 2005-11-11 2007-05-16 Ludwig-Maximilians-Universität München Zielen und Folgen von Antigenen in lebenden Zellen
WO2007067999A2 (en) * 2005-12-09 2007-06-14 Amnis Corporation Extended depth of field imaging for high speed object analysis
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US8344121B2 (en) * 2005-12-12 2013-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Nanoprobes for detection or modification of molecules
SI2347775T1 (sl) 2005-12-13 2020-10-30 President And Fellows Of Harvard College Ogrodja za celično transplantacijo
EP1979000B1 (de) * 2005-12-22 2011-07-13 Memorial Sloan-Kettering Cancer Center Verfahren zur erfassung von krebszellen unter verwendung eines virus
WO2007109749A2 (en) * 2006-03-21 2007-09-27 Wyeth Methods for preventing and treating amyloidogenic diseases
GB0606430D0 (en) * 2006-03-31 2006-05-10 Ge Healthcare Uk Ltd Method for cell based assays
WO2008020334A2 (en) 2006-06-22 2008-02-21 Hannah Monyer Serotonin 5-ht3a receptors in treatment of neurological and psychiatric disorders
US20080004410A1 (en) * 2006-06-30 2008-01-03 Yu-Chin Lai Hydrophilic macromonomers having alpha,beta-conjugated carboxylic terminal group and medical devices incorporating same
WO2008019123A2 (en) 2006-08-04 2008-02-14 Georgia State University Research Foundation, Inc. Enzyme sensors, methods for preparing and using such sensors, and methods of detecting protease activity
US8680235B2 (en) * 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
GB0624462D0 (en) * 2006-12-07 2007-01-17 Ge Healthcare Ltd Method for determining gentoxicity
EP2514767A1 (de) 2006-12-19 2012-10-24 Ablynx N.V. Gegen Metalloproteinase der ADAM-Familie gerichtete Aminosäuren und Polypeptide damit zur Behandlung von Erkrankungen und Störungen im Zusammenhang mit ADAM
US20100062004A1 (en) 2006-12-19 2010-03-11 Ablynx N.V. Amino acid sequences directed against gpcrs and polypeptides comprising the same for the treatment of gpcr-related diseases and disorders
WO2008079228A1 (en) * 2006-12-20 2008-07-03 Danisco Us, Inc., Genencor Division Assays for improved fungal strains
WO2008085502A2 (en) 2007-01-05 2008-07-17 Inseron, Inc. A green fluorescent protein optimized for expression with self-cleaving polypeptides
US8182987B2 (en) * 2007-03-16 2012-05-22 Tokyo Metropolitan Institute Of Medical Science Probe for visualizing cell-cycle
EP2615172A1 (de) 2007-04-27 2013-07-17 Pfenex Inc. Schnellscreeningverfahren für Bakterienwirte zur Identifizierung bestimmter Stämme mit verbessertem Ertrag und/oder verbesserter Qualität bei der Expression heterologer Proteine
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2008150496A2 (en) * 2007-05-31 2008-12-11 Genelux Corporation Assay for sensitivity to chemotherapeutic agents
US9770535B2 (en) * 2007-06-21 2017-09-26 President And Fellows Of Harvard College Scaffolds for cell collection or elimination
US8727554B2 (en) 2007-06-25 2014-05-20 Yorktown Technologies, L.P. Aquarium with adjustable lighting
US20090006219A1 (en) * 2007-06-29 2009-01-01 Alan Blake Method for distribution of an aquarium kit
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
WO2009059305A2 (en) * 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
US8180421B2 (en) 2007-12-12 2012-05-15 Kimberly-Clark Worldwide, Inc. Resonance energy transfer based detection of nosocomial infection
US8619257B2 (en) 2007-12-13 2013-12-31 Kimberley-Clark Worldwide, Inc. Recombinant bacteriophage for detection of nosocomial infection
EP2223086B2 (de) * 2007-12-14 2016-06-01 Biotium Inc. Fluoreszenzverbindungen - markierungsfarbstoffe
EP2078750B1 (de) 2008-01-09 2016-09-28 Ludwig-Maximilians-Universität München Fluoreszenter Zwei-Hybrid(F2H)-Assay zur direkten Sichtbarmachung von Proteininteraktionen in lebenden Zellen
GB0800938D0 (en) * 2008-01-18 2008-02-27 Ge Healthcare Uk Ltd Multiplex cell signalling assay
CN103525751B (zh) * 2008-02-01 2017-04-12 克罗莫塞尔公司 细胞系以及制备和使用其的方法
US9370558B2 (en) 2008-02-13 2016-06-21 President And Fellows Of Harvard College Controlled delivery of TLR agonists in structural polymeric devices
AU2009215188B2 (en) 2008-02-13 2014-09-18 Dana-Farber Cancer Institute, Inc. Continuous cell programming devices
US20090236541A1 (en) * 2008-03-24 2009-09-24 General Electric Company System and Methods for Optical Imaging
AU2009235467A1 (en) 2008-04-07 2009-10-15 Ablynx Nv Single variable domains against the Notch pathways
CA2721076A1 (en) 2008-04-09 2009-10-15 Cobalt Technologies, Inc. Immobilized product tolerant microorganisms
WO2009146456A1 (en) * 2008-05-30 2009-12-03 President And Fellows Of Harvard College Controlled release of growth factors and signaling molecules for promoting angiogenesis
US20100017232A1 (en) * 2008-07-18 2010-01-21 StevenDale Software, LLC Information Transmittal And Notification System
ES2535258T3 (es) * 2008-07-22 2015-05-07 Fibrostatin, Sociedad Limitada Proteína de unión al antígeno de Goodpasture y su detección
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US8962341B2 (en) 2008-12-12 2015-02-24 The Commonwealth Medical College Cell-based detection of APF through its interaction with CKAP4 for diagnosis of interstitial cystitis
CN107011434B (zh) 2008-12-19 2021-02-19 埃博灵克斯股份有限公司 用于产生针对细胞相关抗原如p2x7、cxcr7或cxcr4的免疫球蛋白的基因免疫
WO2010107639A2 (en) 2009-03-20 2010-09-23 Board Of Regents, The University Of Texas System Isolation and characterization of novel green fluorescent proteins from copepods
SG174862A1 (en) 2009-04-10 2011-11-28 Ablynx Nv Improved amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorder
WO2010120749A2 (en) 2009-04-13 2010-10-21 President And Fellow Of Harvard College Harnessing cell dynamics to engineer materials
EP2419121B1 (de) 2009-04-17 2018-07-18 New York University Auf rezeptoren der tnf-familie gerichtete peptide als antagonisten der tnf-wirkung, ihre zusammensetzungen, verfahren und verwendung
KR101852204B1 (ko) 2009-06-05 2018-04-26 아블린쓰 엔.브이. 기도 감염의 예방 및/또는 치료를 위한 일가, 이가 및 삼가 항 인간 호흡기 세포융합 바이러스 (ｈｒｓｖ) 나노바디 구조물
US8497105B2 (en) 2009-06-26 2013-07-30 Cobalt Technologies, Inc. Integrated system and process for bioproduct production
EP2275442A1 (de) 2009-07-06 2011-01-19 Ludwig-Maximilians-Universität München Erkennung und Visualisierung des Zellzyklus von lebenden Zellen
EP2461828B1 (de) 2009-07-31 2017-06-21 President and Fellows of Harvard College Programmierung von zellen für tolerogene therapien
US8815779B2 (en) 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
US8451524B2 (en) * 2009-09-29 2013-05-28 Amnis Corporation Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile
US8658434B2 (en) * 2009-10-28 2014-02-25 Biotium, Inc. Fluorescent pyrene compounds
EP2507262A1 (de) 2009-11-30 2012-10-10 Ablynx N.V. Verbesserte gegen hrsv gerichtete aminosäuresequenzen und polypeptide damit zur prävention und/oder behandlung von atemwegsinfektionen
US9145447B2 (en) * 2009-12-04 2015-09-29 Allele Biotechnology & Pharmaceuticals, Inc. Photoconvertible fluorescent proteins
WO2011083141A2 (en) 2010-01-08 2011-07-14 Ablynx Nv Method for generation of immunoglobulin sequences by using lipoprotein particles
DE102010009445A1 (de) 2010-02-25 2011-08-25 Universitätsklinikum Jena, 07743 Expressionsvektor zur Enzym-Expression in Zellen und Verfahren zu dessen Verwendung
EP2542230A4 (de) 2010-03-05 2013-08-28 Harvard College Förderung des anwachsens von skelettmuskel-stammzellen durch gleichzeitige verabreichung von vegf und igf-1
US8817115B1 (en) 2010-05-05 2014-08-26 Amnis Corporation Spatial alignment of image data from a multichannel detector using a reference image
EP3078753B1 (de) 2010-05-10 2018-04-18 The Regents of The University of California Verfahren unter verwendung von endoribonukleasezusammensetzungen
US9693954B2 (en) 2010-06-25 2017-07-04 President And Fellows Of Harvard College Co-delivery of stimulatory and inhibitory factors to create temporally stable and spatially restricted zones
EP3202415B1 (de) 2010-06-30 2021-12-08 Compugen Ltd. Fusionsprotein beinhaltend c1orf32 und eine oder mehrere domänen einer immunoglobulin heavy chain konstanten region zur behandlung von multipler sklerose, rheumatoider arthritis und anderen autoimmunerkrankungen
AU2011337139B2 (en) 2010-08-30 2015-11-12 President And Fellows Of Harvard College Shear controlled release for stenotic lesions and thrombolytic therapies
US8999335B2 (en) 2010-09-17 2015-04-07 Compugen Ltd. Compositions and methods for treatment of drug resistant multiple myeloma
PT2624873T (pt) 2010-10-06 2020-03-04 Harvard College Hidrogéis injectáveis formadores de poros para terapias celulares à base de materiais
WO2012064697A2 (en) 2010-11-08 2012-05-18 President And Fellows Of Harvard College Materials presenting notch signaling molecules to control cell behavior
EP2678360A1 (de) 2011-02-21 2014-01-01 Fibrostatin, S.L. Verfahren zur behandlung und diagnose von krankheiten
CN103305546B (zh) * 2011-04-20 2014-08-27 上海交通大学 pGreen Max1 真核表达载体及其制备方法
EP2701753B1 (de) 2011-04-27 2018-12-26 President and Fellows of Harvard College Zellfreundliche inverse und opake hydrogele zur zelleinkapselung, zur arzneimittel- und proteinabgabe sowie für funktionelle nanopartikeleinkapselung
EP3417876B1 (de) 2011-04-28 2021-03-31 President and Fellows of Harvard College Injizierbare vorgeformte makroskopische 3d-gerüste zur minimalinvasiven verabreichung
US9675561B2 (en) 2011-04-28 2017-06-13 President And Fellows Of Harvard College Injectable cryogel vaccine devices and methods of use thereof
US9359363B2 (en) 2011-05-20 2016-06-07 Aquinnah Pharmaceuticals, Inc. Identification of compounds that disperse TDP-43 inclusions
JP6062426B2 (ja) 2011-06-03 2017-01-18 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ インサイチュー抗原生成癌ワクチン
US8828928B2 (en) 2011-08-23 2014-09-09 President And Fellows Of Harvard College Amphiphilic peptides and peptide particles
GB201121570D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Methods for producing engineered fluorescent proteins for enhanced fret, products and uses thereof
GB201121565D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Engineering fluorescent proteins for enhanced fret and uses thereof
EP3524676A1 (de) 2011-12-16 2019-08-14 The Board of Trustees of The Leland Stanford Junior University Opsin-polypeptide und verfahren zu ihrer verwendung
ES2953308T3 (es) 2011-12-22 2023-11-10 Harvard College Composiciones y métodos para la detección de analitos
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
WO2013130808A1 (en) 2012-02-29 2013-09-06 D.E. Shaw Research, Llc Methods for screening voltage gated proteins
WO2013138522A2 (en) 2012-03-16 2013-09-19 Genelux Corporation Methods for assessing effectiveness and monitoring oncolytic virus treatment
WO2013158673A1 (en) 2012-04-16 2013-10-24 President And Fellows Of Harvard College Mesoporous silica compositions for modulating immune responses
US20130280170A1 (en) 2012-04-20 2013-10-24 Aladar A. Szalay Imaging methods for oncolytic virus therapy
US9040248B2 (en) 2012-06-06 2015-05-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Kits for detecting and monitoring endocrine disrupting chemicals (EDCs)
EP2866835A1 (de) 2012-07-02 2015-05-06 Fibrostatin, S.L. Gpbp-1 hemmung und ihre therapeutische anwendung
EP2685260A1 (de) 2012-07-09 2014-01-15 Ludwig-Maximilians-Universität München Direkte und quantitative Erkennung von Zielen in lebenden Zellen
RU2015105588A (ru) 2012-07-19 2016-09-10 Редвуд Байосайнс, Инк. Антитело, специфическое к cd22, и способы его применения
US10221221B2 (en) 2012-07-24 2019-03-05 Allele Biotechnology & Pharmaceuticals, Inc Monomeric yellow-green fluorescent protein from cephalochordate
MX359817B (es) 2012-08-16 2018-10-11 Ipierian Inc Anticuerpos que se enlazan a tau.
CN110818798A (zh) 2012-10-25 2020-02-21 美国比奥维拉迪维股份有限公司 抗补体C1s抗体和其用途
LT2914291T (lt) 2012-11-02 2022-06-10 Bioverativ Usa Inc. Antikūnai prieš komplemento c1s ir jų panaudojimo būdai
CA2905432C (en) 2013-03-14 2021-09-14 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
CA2914768A1 (en) 2013-06-10 2014-12-18 Ipierian, Inc. Methods of treating a tauopathy
MX2016006356A (es) 2013-11-27 2016-10-28 Ipierian Inc Metodos de tratamiento de una tauopatia.
US9682123B2 (en) 2013-12-20 2017-06-20 The Trustees Of Columbia University In The City Of New York Methods of treating metabolic disease
CA2945945C (en) 2014-04-15 2023-10-03 The Regents Of The University Of California Bi-terminal pegylated integrin-binding peptides and methods of use thereof
EP3137105A4 (de) 2014-04-30 2017-12-27 President and Fellows of Harvard College Kombinationsimpfstoffvorrichtungen und -verfahren zur abtötung von krebszellen
EP3151866B1 (de) 2014-06-09 2023-03-08 Voyager Therapeutics, Inc. Chimäre kapside
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
WO2016007886A1 (en) 2014-07-11 2016-01-14 Northwestern University Yeast-based biosensor
EP3215191A4 (de) 2014-11-05 2018-08-01 Voyager Therapeutics, Inc. Aadc-polynukleotide zur behandlung von morbus parkinson
WO2016077259A1 (en) 2014-11-12 2016-05-19 Georgia State University Research Foundation, Inc. Surgical articles and methods for detection
CN114717264A (zh) 2014-11-14 2022-07-08 沃雅戈治疗公司 治疗肌萎缩性侧索硬化(als)的组合物和方法
JP6863891B2 (ja) 2014-11-14 2021-04-21 ボイジャー セラピューティクス インコーポレイテッドＶｏｙａｇｅｒ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 調節性ポリヌクレオチド
WO2016090317A1 (en) 2014-12-05 2016-06-09 Aquinnah Pharmaceuticals, Inc. Sulfonamide derivatives, compositions and methods of use in the treatment of neurodegenerative diseases
CA2969699A1 (en) 2014-12-05 2016-06-09 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use
US11697825B2 (en) 2014-12-12 2023-07-11 Voyager Therapeutics, Inc. Compositions and methods for the production of scAAV
WO2016115503A1 (en) 2015-01-16 2016-07-21 Voyager Therapeutics, Inc. Central nervous system targeting polynucleotides
WO2016123573A1 (en) 2015-01-30 2016-08-04 President And Fellows Of Harvard College Peritumoral and intratumoral materials for cancer therapy
JP7094533B2 (ja) 2015-04-10 2022-07-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 免疫細胞捕捉デバイスおよびその製造および使用方法
WO2016186948A1 (en) 2015-05-15 2016-11-24 Albert Einstein College Of Medicine, Inc. Gfp-derived fusion tags for protein expression
WO2017009712A1 (en) 2015-07-13 2017-01-19 Compugen Ltd. Hide1 compositions and methods
US20180180595A1 (en) * 2015-07-24 2018-06-28 The Regents Of The University Of California Cell lines mutant for patched 1 and patched 2 and methods of use thereof
KR20180042412A (ko) 2015-09-02 2018-04-25 이뮤텝 에스.에이.에스. 항-lag-3 항체
CA2997947A1 (en) 2015-09-09 2017-03-16 The Trustees Of Columbia University In The City Of New York Reduction of er-mam-localized app-c99 and methods of treating alzheimer's disease
US20180305334A1 (en) 2015-10-14 2018-10-25 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use against stress granules
US20180312534A1 (en) 2015-10-16 2018-11-01 Invictus Oncology Pvt. Ltd. Fluorescent anticancer platinum drugs
WO2017075335A1 (en) 2015-10-28 2017-05-04 Voyager Therapeutics, Inc. Regulatable expression using adeno-associated virus (aav)
US10983110B2 (en) 2015-12-02 2021-04-20 Voyager Therapeutics, Inc. Assays for the detection of AAV neutralizing antibodies
US11752238B2 (en) 2016-02-06 2023-09-12 President And Fellows Of Harvard College Recapitulating the hematopoietic niche to reconstitute immunity
US11299751B2 (en) 2016-04-29 2022-04-12 Voyager Therapeutics, Inc. Compositions for the treatment of disease
EP3448874A4 (de) 2016-04-29 2020-04-22 Voyager Therapeutics, Inc. Zusammensetzungen zur behandlung einer erkrankung
WO2017201258A1 (en) 2016-05-18 2017-11-23 Voyager Therapeutics, Inc. Compositions and methods of treating huntington's disease
BR112018073384A2 (pt) 2016-05-18 2019-03-06 Voyager Therapeutics, Inc. polinucleotídeos moduladores
US11555177B2 (en) 2016-07-13 2023-01-17 President And Fellows Of Harvard College Antigen-presenting cell-mimetic scaffolds and methods for making and using the same
EP3506817A4 (de) 2016-08-30 2020-07-22 The Regents of The University of California Verfahren für biomedizinisches targeting und freisetzung sowie vorrichtungen und systeme zur ausführung davon
JP7222888B2 (ja) 2016-11-16 2023-02-15 アブリンクス エン．ヴェー． Ｃｄ１２３及びｔｃｒアルファ／ベータに結合することが可能なｔ細胞動員ポリペプチド
WO2018204786A1 (en) 2017-05-05 2018-11-08 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (als)
US11752181B2 (en) 2017-05-05 2023-09-12 Voyager Therapeutics, Inc. Compositions and methods of treating Huntington's disease
JP7229989B2 (ja) 2017-07-17 2023-02-28 ボイジャー セラピューティクス インコーポレイテッド 軌道アレイガイドシステム
MX2020001187A (es) 2017-08-03 2020-10-05 Voyager Therapeutics Inc Composiciones y métodos para la administración de virus adenoasociados.
WO2019079240A1 (en) 2017-10-16 2019-04-25 Voyager Therapeutics, Inc. TREATMENT OF AMYOTROPHIC LATERAL SCLEROSIS (ALS)
WO2019094702A1 (en) 2017-11-10 2019-05-16 Cocoon Biotech Inc. Ocular applications of silk-based products
US20200392512A1 (en) 2018-02-26 2020-12-17 Ablynx N.V. Nucleotide sequences encoding peptide linkers
EP3762500A1 (de) 2018-03-06 2021-01-13 Voyager Therapeutics, Inc. Aus insektenzelle hergestellte teilweise selbstkomplementäre aav-genome
CN112313331A (zh) 2018-04-27 2021-02-02 沃雅戈治疗公司 用于测量aadc病毒载体效力的方法
US20210230632A1 (en) 2018-05-15 2021-07-29 Voyager Therapeutics, Inc. Compositions and methods for delivery of aav
BR112020023082A2 (pt) 2018-05-15 2021-02-09 Voyager Therapeutics, Inc. composições e métodos para o tratamento de doença de parkinson
EP3793686A1 (de) 2018-05-16 2021-03-24 Voyager Therapeutics, Inc. Aav-serotypen zur gehirnspezifischen payload-übertragung
WO2019222444A2 (en) 2018-05-16 2019-11-21 Voyager Therapeutics, Inc. Directed evolution
WO2020069461A1 (en) 2018-09-28 2020-04-02 Voyager Therapeutics, Inc. Frataxin expression constructs having engineered promoters and methods of use thereof
GB201817321D0 (en) 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
US20240124889A1 (en) 2019-05-07 2024-04-18 Voyager Therapeutics, Inc. Compositions and methods for the vectored augmentation of protein destruction, expression and/or regulation
BR102019017792A2 (pt) 2019-08-27 2021-11-16 Fundação Oswaldo Cruz Receptáculo proteico, método para produção do receptáculo, método de identificação de patógenos ou de diagnóstico de doenças, e, uso do receptáculo
WO2021046155A1 (en) 2019-09-03 2021-03-11 Voyager Therapeutics, Inc. Vectorized editing of nucleic acids to correct overt mutations
CA3180222A1 (en) 2020-04-15 2021-10-21 Voyager Therapeutics, Inc. Tau binding compounds
WO2021212096A1 (en) 2020-04-17 2021-10-21 Regeneron Pharmaceuticals, Inc. Detection assays for coronavirus neutralizing antibodies
US20230203102A1 (en) 2020-05-13 2023-06-29 Voyager Therapeutics, Inc. Redirection of tropism of aav capsids
US20230285596A1 (en) 2020-07-27 2023-09-14 Voyager Therapeutics, Inc Compositions and methods for the treatment of niemann-pick type c1 disease
CA3190309A1 (en) 2020-07-27 2022-02-03 Voyager Therapeutics, Inc. Compositions and methods for the treatment of neurological disorders related to glucosylceramidase beta deficiency
WO2023044483A2 (en) 2021-09-20 2023-03-23 Voyager Therapeutics, Inc. Compositions and methods for the treatment of her2 positive cancer
WO2023091949A2 (en) 2021-11-17 2023-05-25 Voyager Therapeutics, Inc. Compositions and methods for the treatment of neurological disorders related to glucosylceramidase beta deficiency
CN116143893B (zh) * 2022-08-09 2023-11-10 无锡佰翱得生物科学股份有限公司 一种增强型单体StayGold蛋白及其应用
WO2024059739A1 (en) 2022-09-15 2024-03-21 Voyager Therapeutics, Inc. Tau binding compounds

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL60888A (en) 1978-06-16 1984-07-31 Yeda Res & Dev Substrates and process for the quantitative assay of enzymes
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5070012A (en) 1988-03-30 1991-12-03 The Board Of Trustees Of The Leland Stanford Junior University Monitoring of cells and trans-activating transcription elements
US5134232A (en) 1988-11-14 1992-07-28 The Regents Of The University Of California Fluorescent indicator dyes for alkali metal cations
GB8916806D0 (en) 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
EP0428000A1 (de) 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
WO1992000388A1 (en) 1990-07-02 1992-01-09 The Regents Of The University Of California Detection of analytes using fluorescent energy transfer
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
DE4105354A1 (de) * 1991-02-21 1992-08-27 Basf Ag Waessrige polymerdispersionen, enthaltend organische verbindungen mit carbonat- und carbonylgruppen, sowie damit hergestellte anstrich- und beschichtungssysteme
GB9310978D0 (en) 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US5491084A (en) 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995007463A1 (en) 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
WO1995021191A1 (en) * 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
ES2170220T3 (es) 1995-01-31 2002-08-01 Bioimage As Procedimiento para detectar sustancias biologicamente activas.
WO1996027027A1 (en) 1995-03-02 1996-09-06 Rutgers The State University Of New Jersey Improved method for purifying green fluorescent protein
GB9504446D0 (en) 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
CA2232727C (en) 1995-09-22 2002-03-26 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6140132A (en) * 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample

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US20020123113A1 (en) 2002-09-05
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ATE201878T1 (de) 2001-06-15
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US5625048A (en) 1997-04-29
US6319669B1 (en) 2001-11-20
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