DE69521219D1 - Modifizierte grün fluorszierende proteine - Google Patents

Modifizierte grün fluorszierende proteine

Info

Publication number
DE69521219D1
DE69521219D1 DE69521219T DE69521219T DE69521219D1 DE 69521219 D1 DE69521219 D1 DE 69521219D1 DE 69521219 T DE69521219 T DE 69521219T DE 69521219 T DE69521219 T DE 69521219T DE 69521219 D1 DE69521219 D1 DE 69521219D1
Authority
DE
Germany
Prior art keywords
wild
gfp
product derived
modifications
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69521219T
Other languages
English (en)
Other versions
DE69521219T2 (de
Inventor
Y Tsien
Roger Heim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23322562&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69521219(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of California filed Critical University of California
Publication of DE69521219D1 publication Critical patent/DE69521219D1/de
Application granted granted Critical
Publication of DE69521219T2 publication Critical patent/DE69521219T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/855Proteins from animals other than mammals or birds
DE69521219T 1994-11-10 1995-11-13 Modifizierte grün fluorszierende proteine Expired - Lifetime DE69521219T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/337,915 US5625048A (en) 1994-11-10 1994-11-10 Modified green fluorescent proteins
PCT/US1995/014692 WO1996023810A1 (en) 1994-11-10 1995-11-13 Modified green fluorescenct proteins

Publications (2)

Publication Number Publication Date
DE69521219D1 true DE69521219D1 (de) 2001-07-12
DE69521219T2 DE69521219T2 (de) 2001-10-25

Family

ID=23322562

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69521219T Expired - Lifetime DE69521219T2 (de) 1994-11-10 1995-11-13 Modifizierte grün fluorszierende proteine

Country Status (12)

Country Link
US (4) US5625048A (de)
EP (2) EP0804457B1 (de)
JP (1) JP3283523B2 (de)
AT (1) ATE201878T1 (de)
AU (1) AU702205B2 (de)
CA (1) CA2205006C (de)
DE (1) DE69521219T2 (de)
DK (2) DK0804457T3 (de)
ES (1) ES2159650T3 (de)
GR (1) GR3036264T3 (de)
PT (1) PT804457E (de)
WO (1) WO1996023810A1 (de)

Families Citing this family (358)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
DE69604298T2 (de) * 1995-09-22 2000-05-18 Bioimage A S Soeborg Varianten des grünen fluoreszenzproteins, gfp
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US6020192A (en) * 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US6900304B2 (en) 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US5804387A (en) * 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6027881A (en) * 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US5912137A (en) * 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
AU767375B2 (en) * 1996-08-16 2003-11-06 Aurora Biosciences Corporation Long wavelength engineered fluorescent proteins
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
WO1998021355A1 (en) * 1996-11-15 1998-05-22 Life Technologies, Inc. Mutants of green fluorescent protein
AU741076B2 (en) 1996-12-12 2001-11-22 Prolume, Ltd. Apparatus and method for detecting and identifying infectious agents
US6090919A (en) * 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5994077A (en) * 1997-01-31 1999-11-30 The Board Of Trustees Of The Leland Stanford Junior University Flourescence-based isolation of differentially induced genes
US6670449B1 (en) 1997-02-13 2003-12-30 Memorial Sloan-Kettering Cancer Center Hybrid molecules and their use for optically detecting changes in cellular microenvironments
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
DE19715683C2 (de) * 1997-04-15 1999-11-11 Max Planck Gesellschaft Neue Reportervektoren für One-Hybrid und Two-Hybrid Systeme
US7541151B2 (en) 1997-06-05 2009-06-02 Duke University Single-cell biosensor for the measurement of GPCR ligands in a test sample
US5891646A (en) 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
FR2764387B1 (fr) * 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US6528271B1 (en) * 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6130313A (en) * 1997-10-02 2000-10-10 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins
US6265174B1 (en) 1997-11-03 2001-07-24 Morphochem, Inc. Methods and compositions for identifying and modulating ctionprotein-interactions
ATE426036T1 (de) * 1997-11-14 2009-04-15 Cedars Sinai Medical Center Transfektion und transfer nicht humaner mannlicher keimzellen zur generierung transgener nicht humaner saugetiere
US6734338B1 (en) 1997-11-14 2004-05-11 Cedars-Sinai Medical Center Transfection, storage and transfer of male germ cells for generation of transgenic species and genetic therapies
US7294755B1 (en) 1997-11-14 2007-11-13 Cedars-Sinai Medical Center Genetic modification of male germ cells for generation of transgenic species and genetic therapies
US6194548B1 (en) * 1998-01-23 2001-02-27 Takashi Osumi Green fluorescent proteins and blue fluorescent proteins
EP1950223A3 (de) * 1998-03-09 2009-05-13 Zealand Pharma A/S Pharmakologisch aktive Peptidkonjugate mit verringerter enzymatischer Hydrolysetendenz
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
EP1064360B1 (de) 1998-03-27 2008-03-05 Prolume, Ltd. Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6080576A (en) * 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
EP0949269A1 (de) * 1998-04-02 1999-10-13 SymBiosis GmbH Biosensor Protein
US6197947B1 (en) 1998-06-01 2001-03-06 The Rockefeller University Translation initiation factor 4AIII and methods of use thereof
US6150176A (en) 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6140132A (en) * 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6495664B1 (en) * 1998-07-24 2002-12-17 Aurora Biosciences Corporation Fluorescent protein sensors of post-translational modifications
WO2000008443A1 (en) * 1998-08-08 2000-02-17 Imperial Cancer Research Technology Limited Multiple frequency fluorescence lifetime imaging
US7297482B2 (en) * 1998-10-08 2007-11-20 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
US8012682B2 (en) * 1998-12-11 2011-09-06 Clontech Laboratories, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US9834584B2 (en) * 1998-12-11 2017-12-05 Takara Bio Usa, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US20030087492A1 (en) 2001-11-02 2003-05-08 Promos Technologies, Inc. Semiconductor device and method of manufacturing the same
US20020103359A1 (en) * 1999-12-07 2002-08-01 Gregory Donoho Novel human membrane proteins and polynucleotides encoding the same
US20020031802A1 (en) * 2000-05-12 2002-03-14 Yi Hu Novel seven transmembrane proteins and polynucleotides encoding the same
US7450229B2 (en) * 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US20060257884A1 (en) * 2004-05-20 2006-11-16 Amnis Corporation Methods for preparing and analyzing cells having chromosomal abnormalities
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8005314B2 (en) * 2005-12-09 2011-08-23 Amnis Corporation Extended depth of field imaging for high speed object analysis
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
JP2002536009A (ja) * 1999-02-09 2002-10-29 レキシコン・ジェネティクス・インコーポレーテッド ヒト脱共役タンパク質およびそれをコードするポリヌクレオチド
US6670144B1 (en) 1999-02-26 2003-12-30 Cyclacel, Ltd. Compositions and methods for monitoring the phosphorylation of natural binding partners
US8148110B2 (en) 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
DE19915137C2 (de) * 1999-03-26 2001-10-18 Michael Schaefer Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
WO2001009177A2 (en) * 1999-07-29 2001-02-08 Whitehead Institute For Biomedical Research Affinity fluorescent proteins and uses thereof
NL1012782C2 (nl) * 1999-08-05 2001-02-06 Keygene Nv Verbeterde gfp-variant, bruikbaar als markering, en gfp-construct.
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6716614B1 (en) 1999-09-02 2004-04-06 Lexicon Genetics Incorporated Human calcium dependent proteases, polynucleotides encoding the same, and uses thereof
JP2003508076A (ja) * 1999-09-08 2003-03-04 レキシコン・ジェネティクス・インコーポレーテッド ヒトgタンパク質と共役する7回膜貫通受容体およびそれをコードするポリヌクレオチド
US6861220B2 (en) 1999-09-08 2005-03-01 Ramot University Authority For Applied Research & Industrial Development Ltd Genetic screening methods
US6541252B1 (en) 2000-05-19 2003-04-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
AU782326B2 (en) 1999-10-12 2005-07-21 Lexicon Pharmaceuticals, Inc. Human LDL receptor family proteins and polynucleotides encoding the same
WO2001027275A1 (en) * 1999-10-13 2001-04-19 Lexicon Genetics Incorporated Novel human membrane proteins
EP2308974A3 (de) 1999-10-14 2011-08-10 Clontech Laboratories Inc. Aus Anthozoa abgeleitete Chrom-/Fluorproteine sowie Verwendungsverfahren dafür
JP2004500045A (ja) * 1999-10-18 2004-01-08 レキシコン・ジェネティクス・インコーポレーテッド 新規ヒトタンパクおよびそれをコードするポリヌクレオチド
WO2001051634A1 (en) * 2000-01-12 2001-07-19 Lexicon Genetics Incorporated Human olfactory receptor and polynucleotides encoding the same
JP2003529348A (ja) * 2000-02-04 2003-10-07 レキシコン・ジェネティクス・インコーポレーテッド 新規ヒト膜タンパクおよびそれをコードするポリヌクレオチド
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
JP4342069B2 (ja) * 2000-02-14 2009-10-14 生化学工業株式会社 α1,4−ガラクトース転移酵素およびそれをコードするDNA
ATE439441T1 (de) * 2000-02-23 2009-08-15 Life Technologies Corp Veränderte, fluoreszierende proteine
US20020064842A1 (en) * 2000-06-09 2002-05-30 Sorge Joseph A. Renilla reniformis green fluorescent protein and mutants thereof
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US7029878B2 (en) * 2000-03-15 2006-04-18 Merck & Co., Inc. Melanin concentrating hormone receptor chimeric and fusion proteins
WO2001081411A2 (en) * 2000-04-27 2001-11-01 Lexicon Genetics Incorporated Human 7-transmembrane g-protein coupled receptors and polynucleotides encoding the same
US6686188B2 (en) * 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
US6656700B2 (en) * 2000-05-26 2003-12-02 Amersham Plc Isoforms of human pregnancy-associated protein-E
US6790667B1 (en) * 2000-05-30 2004-09-14 Lexicon Genetics Incorporated Human mitochondrial proteins and polynucleotides encoding the same
WO2001094417A2 (en) * 2000-06-09 2001-12-13 Lexicon Genetics Incorporated Novel human seven transmembrane proteins and polynucleotides encoding the same
DE60139556D1 (de) * 2000-06-19 2009-09-24 Fisher Bioimage Aps Fluoreszierende proteine
US6667153B1 (en) 2000-06-26 2003-12-23 Susan Margaret Thomas Composition and method for detecting mutagens
US6806289B1 (en) 2000-07-14 2004-10-19 Stephen J. Lippard Coordination complexes, and methods for preparing by combinatorial methods, assaying and using the same
US20020123474A1 (en) * 2000-10-04 2002-09-05 Shannon Mark E. Human GTP-Rho binding protein2
EP1334122B1 (de) * 2000-10-12 2006-08-23 Clontech Laboratories, Inc. Nukleinsäuren, die für stichodactylidae chromoproteine kodieren
AU2001297843A1 (en) 2000-10-12 2002-12-23 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
CA2427113A1 (en) * 2000-10-27 2002-09-12 Lexicon Genetics Incorporated Novel human 7tm proteins and polynucleotides encoding the same
EP1330524A2 (de) * 2000-10-30 2003-07-30 Lexicon Genetics Incorporated Humane 7tm-proteine und dafür kodierende polynukleotiden
US7163800B2 (en) * 2000-11-03 2007-01-16 Molecular Devices Corporation Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US6977293B1 (en) 2000-11-03 2005-12-20 Ceres, Inc. Chimeric polypeptides
US20050136431A1 (en) * 2000-11-03 2005-06-23 Oakley Robert H. Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7018812B2 (en) * 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
US7279324B2 (en) * 2001-01-23 2007-10-09 Duke University Nucleic acid encoding G-protein coupled receptor with modified DRY motif
CA2434293A1 (en) * 2001-01-29 2002-08-08 Anticancer, Inc. Fluorescent proteins
US6969597B2 (en) * 2001-02-21 2005-11-29 Clontech Laboratories, Inc. Nucleic acids encoding non aggregating fluorescent proteins and methods for using the same
US7687614B2 (en) 2001-02-26 2010-03-30 The Regents Of The University Of California Monomeric and dimeric fluorescent protein variants and methods for making same
JP4215512B2 (ja) * 2001-02-26 2009-01-28 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 非オリゴマー化タンデム蛍光性タンパク質
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
JP3929250B2 (ja) * 2001-03-08 2007-06-13 株式会社ルネサステクノロジ 半導体装置
DE02708018T1 (de) * 2001-03-09 2004-09-30 Gene Stream Pty. Ltd. Neue expressionsvektoren
WO2002077011A2 (en) * 2001-03-12 2002-10-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation-reduction sensitive green fluorescent protein variants
GB0109858D0 (en) 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
WO2002096924A1 (en) * 2001-05-25 2002-12-05 Clontech Laboratories, Inc. Kindling fluorescent proteins and methods for their use
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
US20030100031A1 (en) * 2001-06-11 2003-05-29 Steven Dower Integrative assays for monitoring molecular assembly events
AU2002322416A1 (en) * 2001-07-06 2003-03-03 Board Of Trustees Of The University Of Illinois Gfp fusion proteins and their use
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
WO2003013437A2 (en) * 2001-08-07 2003-02-20 University Of Delaware Compositions and methods for the prevention and treatment of huntington's disease
US20040096880A1 (en) * 2001-08-07 2004-05-20 Kmiec Eric B. Compositions and methods for the treatment of diseases exhibiting protein misassembly and aggregation
AU2002324769A1 (en) * 2001-08-22 2003-03-10 University Of Hawaii Physalia fluorescent proteins
KR20040066788A (ko) 2001-08-31 2004-07-27 더 락커펠러 유니버시티 프스포다이에스터레이즈 활성 및 뇌에서프스포다이에스터레이즈 1b 매개되는 신호의 조절
DE60223937T2 (de) * 2001-10-12 2008-11-27 Clontech Laboratories, Inc., Mountain View Nukleinsäuren kodierend miteinander verbundene chromo/fluoreszenzdomänen und verfahren zu ihrer verwendung
WO2003040312A2 (en) * 2001-10-24 2003-05-15 University Of Hawaii Multifunctional green fluorescent protein and uses thereof
DE10153829A1 (de) * 2001-11-05 2003-05-28 Bayer Ag Assay basierend auf dotierten Nanoteilchen
WO2003042401A2 (en) * 2001-11-13 2003-05-22 Clontech Laboratories, Inc. Novel chromophores/fluorophores and methods for using the same
US20030104439A1 (en) * 2001-11-30 2003-06-05 Finch Rosalynde J. Methods of identifying cellular target molecules
EP1456223B1 (de) * 2001-12-19 2009-08-12 The University Of Chicago Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
JP2005514032A (ja) * 2001-12-26 2005-05-19 クローンテック ラボラトリーズ インク. 変異体発色団/蛍光団ならびにそれらの製造法および使用法
RU2330886C2 (ru) * 2002-01-22 2008-08-10 Закрытое Акционерное Общество "Евроген Айпи" НОВЫЕ ФЛУОРЕСЦИРУЮЩИЕ БЕЛКИ Aequorea coerulscens И СПОСОБЫ ИХ ПРИМЕНЕНИЯ
US6918839B2 (en) * 2002-01-28 2005-07-19 The Boeing Company Damage tolerant shaft
US20040005301A1 (en) * 2002-02-12 2004-01-08 Goldman Steven A. Identification and high-yield isolation of human pancreatic islet progenitor and stem cells
AU2003219839B2 (en) 2002-02-21 2008-02-21 Medimmune, Llc Recombinant parainfluenza virus expression systems and vaccines comprising heterologous antigens derived from metapneumovirus
KR100468225B1 (ko) * 2002-03-08 2005-01-26 (주)뉴로제넥스 형광강도가 증강된 삽입형 녹색 형광 단백질 및 그유전자
US20030170611A1 (en) * 2002-03-09 2003-09-11 Cardone Michael H. Cell-based screening methods
US6730492B2 (en) 2002-03-09 2004-05-04 Merrimack Pharmaceuticals, Inc. Cell-based screening methods
US20030170850A1 (en) * 2002-03-09 2003-09-11 Cardone Michael H. Cell-based screening methods
US20030182669A1 (en) * 2002-03-19 2003-09-25 Rockman Howard A. Phosphoinositide 3-kinase mediated inhibition of GPCRs
KR20030076735A (ko) * 2002-03-21 2003-09-29 김경진 변종삽입형광단백질
GB0207533D0 (en) 2002-04-02 2002-05-08 Oxford Glycosciences Uk Ltd Protein
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
US7666587B2 (en) * 2002-04-12 2010-02-23 New York University Method of screening test substances for treating or preventing a disease mediated by plasma cells
US7271241B2 (en) 2002-04-24 2007-09-18 Los Alamos National Security, Llc Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby
FR2851379B1 (fr) * 2003-02-18 2008-02-01 Cit Alcatel Convertisseur en transfert direct d'energie
DE60325719D1 (de) * 2002-05-13 2009-02-26 Molecular Devices Corp Konstitutiv translozierende zelllinie
US7351542B2 (en) 2002-05-20 2008-04-01 The Regents Of The University Of California Methods of modulating tubulin deacetylase activity
US7290215B2 (en) * 2002-06-03 2007-10-30 Microsoft Corporation Dynamic wizard interface system and method
DK1534819T3 (da) 2002-08-21 2010-04-19 Revivicor Inc Griselignende dyr, som mangler enhver ekspression af funktionel alfa-1,3-galactosyltransferase
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2004042019A2 (en) * 2002-10-30 2004-05-21 Pointilliste, Inc. Systems for capture and analysis of biological particles and methods using the systems
US7056683B2 (en) * 2002-11-12 2006-06-06 Massachusetts Institute Of Technology Genetically encoded fluorescent reporters of kinase, methyltransferase, and acetyl-transferase activities
WO2004044203A1 (en) 2002-11-12 2004-05-27 Zakrytoe Aktsionernoe Obschestvo 'evrogen' Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same
AU2003298674A1 (en) * 2002-11-14 2004-06-15 Genentech Inc Intron fusion construct and method of using for selecting high-expressing production cell lines
DE03781176T1 (de) 2002-12-26 2006-06-22 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluoreszenzproteine aus copepoda-speziesund verfahren zur verwendung davon
WO2004067717A2 (en) * 2003-01-24 2004-08-12 Duke University Modified trafficking patterns for arrestin and g-protein-coupled receptors via arrestin-ubiquitin chimera
US20040241748A1 (en) * 2003-02-10 2004-12-02 Dana Ault-Riche Self-assembling arrays and uses thereof
EP1613160A4 (de) 2003-03-27 2008-01-02 Ptc Therapeutics Inc Betrachtung von enzymen des trna-splice-wegs zur identifikation von antifungalen und/oder proliferationshemmenden molekülen
US20070020630A1 (en) 2003-03-27 2007-01-25 Trotta Christopher R Methods of identifying compounds that target trna splicing endonuclease and uses of said compounds as anti-proliferative agents
WO2004090115A2 (en) * 2003-04-04 2004-10-21 Stratagene Renilla gfp mutants with increased fluorescent intensity and special shift
US7160698B2 (en) * 2003-05-22 2007-01-09 University Of Florida Research Foundation, Inc. Fluorescent and colored proteins, and polynucleotides that encode these proteins
ATE506456T1 (de) 2003-06-10 2011-05-15 David Gladstone Inst Verfahren zur behandlung von lentivirusinfektionen
US7619059B2 (en) * 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (en) * 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US20050037364A1 (en) * 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
EP1668362A4 (de) * 2003-08-13 2007-07-04 Burnham Inst Zytoplasmatische aktivität des retinoid-x-rezeptors und seine regulation durch liganden und dimerisierung
CA2538797A1 (en) * 2003-09-12 2005-03-24 Invitrogen Corporation Multiplex binding and activity assays
RU2006114432A (ru) * 2003-09-30 2007-11-20 Лексикон Дженетикс Инкорпорейтед (Us) Способы и композиции для определения функции гена
WO2005074436A2 (en) 2003-10-24 2005-08-18 The Regents Of The University Of California Self-assembling split-fluorescent protein systems
US20050095648A1 (en) * 2003-10-30 2005-05-05 Mario Geysen Method for designing linear epitopes and algorithm therefor and polypeptide epitopes
US20050287668A1 (en) * 2003-11-04 2005-12-29 Cell Therapeutics, Inc. (Cti) RNA interference compositions and screening methods for the identification of novel genes and biological pathways
WO2005059102A2 (en) * 2003-12-10 2005-06-30 University Of Utah Research Foundation METHOD FOR OBTAINING AN ENRICHED POPULATION OF siRNA-EXPRESSING CELLS
WO2005067980A2 (en) * 2004-01-12 2005-07-28 Pointilliste, Inc. Design of therapeutics and therapeutics
EP1728077A4 (de) 2004-01-30 2008-11-05 Dana Farber Cancer Inst Inc Verfahren zum bestimmen und quantifizieren einer strahlungs- oder genotoxinbelastung
US8103080B2 (en) * 2004-03-16 2012-01-24 Amnis Corporation Method for imaging and differential analysis of cells
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells
EP1800124B1 (de) 2004-03-16 2011-12-21 Amnis Corporation Auf bildlicher darstellung beruhende quantifizierung molekularer translokation
WO2005100387A1 (en) 2004-04-07 2005-10-27 The University Of Chicago Monomeric red fluorescent proteins
US7504205B2 (en) * 2004-05-17 2009-03-17 The Burnham Institute Uncharacterized ORF3 in SARS-coronavirus is a cyclic-AMP-dependent kinase and a target for SARS therapy
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
US20110039254A1 (en) 2004-09-14 2011-02-17 Pinheiro Leonardo B Chromosomal Insertion of Gfp Into Bacteria For Quality Control
US8354093B2 (en) 2004-10-15 2013-01-15 Washington University Cell permeable nanoconjugates of shell-crosslinked knedel (SCK) and peptide nucleic acids (“PNAs”) with uniquely expressed or over-expressed mRNA targeting sequences for early diagnosis and therapy of cancer
US7709208B2 (en) * 2004-11-08 2010-05-04 New York University Methods for diagnosis of major depressive disorder
US7393923B2 (en) 2004-11-08 2008-07-01 The Regents Of The University Of California Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same
EP1820024B1 (de) 2004-12-01 2012-06-13 Proteologics, Ltd Ubiquitin-ligase-assays und verwandte reagenzien
US7230080B2 (en) * 2005-02-16 2007-06-12 University Of Florida Research Foundation, Inc. Fluorescent and colored proteins, and polynucleotides that encode these proteins
DK1920248T3 (da) 2005-04-05 2010-12-13 Allergan Inc Clostridialt-toksin-aktivitetsassays
HUE045710T2 (hu) 2005-05-18 2020-01-28 Ablynx Nv Javított, alfa tumor nekrózis faktor elleni nanobodies
EP3415535B1 (de) 2005-05-20 2020-12-09 Ablynx N.V. Verbesserte nanokörper (tm) zur behandlung von aggregationsvermittelten erkrankungen
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
WO2007009058A2 (en) 2005-07-13 2007-01-18 Georgia State University Research Foundation, Inc. Contrast agents and methods for preparing contrast agents
CA2617930A1 (en) 2005-08-09 2007-03-29 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
JP5377965B2 (ja) 2005-09-09 2013-12-25 ジョージア ステート ユニバーシティ リサーチ ファウンデーション、インコーポレイテッド 標的化された造影剤および造影剤を標的化するための方法
EP1945795B1 (de) * 2005-09-28 2016-08-10 Attagene, Inc. Verfahren und konstrukte zur analyse biologischer aktivitäten biologischer proben und zur bestimmung von zuständen eines organismus
EP1931787A4 (de) * 2005-09-30 2010-10-20 Univ Montana State System zum nachweis von protein-protein-wechselwirkungen
ES2399563T3 (es) 2005-11-04 2013-04-02 Evrogen, Jsc Proteínas verdes fluorescentes modificadas y método para la utilización de las mismas
EP1785434A1 (de) 2005-11-11 2007-05-16 Ludwig-Maximilians-Universität München Zielen und Folgen von Antigenen in lebenden Zellen
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
DE602006016984D1 (de) 2005-12-12 2010-10-28 Us Gov Health & Human Serv Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren
CA2632630C (en) 2005-12-13 2016-12-06 President And Fellows Of Harvard College Scaffolds for cell transplantation
CA2634591A1 (en) * 2005-12-22 2007-07-05 Memorial Sloan-Kettering Cancer Center Method for detection of cancer cells using virus
RU2008134135A (ru) * 2006-03-21 2010-04-27 Вайет (Us) Соединения-антагонисты белка rage и способы их применения
GB0606430D0 (en) * 2006-03-31 2006-05-10 Ge Healthcare Uk Ltd Method for cell based assays
US20100297102A1 (en) 2006-06-22 2010-11-25 Hannah Monyer Serotonin 5-ht3a receptors in treatment of neurological and psychiatric disorders
US20080004410A1 (en) * 2006-06-30 2008-01-03 Yu-Chin Lai Hydrophilic macromonomers having alpha,beta-conjugated carboxylic terminal group and medical devices incorporating same
EP2518155B1 (de) 2006-08-04 2014-07-23 Georgia State University Research Foundation, Inc. Enzymsensoren, Verfahren zur Herstellung und Anwendung Solcher Sensoren Sowie Verfahren zur Erkennung von Protease-Aktivität
US8680235B2 (en) * 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
GB0624462D0 (en) * 2006-12-07 2007-01-17 Ge Healthcare Ltd Method for determining gentoxicity
AU2007336243B2 (en) 2006-12-19 2012-07-26 Ablynx N.V. Amino acid sequences directed against a metalloproteinase from the ADAM family and polypeptides comprising the same for the treatment of ADAM-related diseases and disorders
CA2673331A1 (en) 2006-12-19 2008-06-26 Ablynx N.V. Amino acid sequences directed against gpcrs and polypeptides comprising the same for the treatment of gpcr-related diseases and disorders
US20100093061A1 (en) * 2006-12-20 2010-04-15 Bodie Elizabeth A Assays for Improved Fungal Strains
EP2104681A4 (de) * 2007-01-05 2011-01-19 Inseron Inc Für die expression mit selbstspaltenden polypeptiden optimiertes grünfluoreszierendes protein
EP2138577B1 (de) * 2007-03-16 2017-03-22 Tokyo Metropolitan Institute of Medical Science Sonde zur sichtbarmachung des zellzyklus
BRPI0810120A2 (pt) 2007-04-27 2014-11-11 Dow Global Technologies Inc Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2008150496A2 (en) * 2007-05-31 2008-12-11 Genelux Corporation Assay for sensitivity to chemotherapeutic agents
WO2009002401A2 (en) 2007-06-21 2008-12-31 President And Fellows Of Harvard College Scaffolds for cell collection or elimination
US8727554B2 (en) 2007-06-25 2014-05-20 Yorktown Technologies, L.P. Aquarium with adjustable lighting
US20090006219A1 (en) * 2007-06-29 2009-01-01 Alan Blake Method for distribution of an aquarium kit
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
US8679749B2 (en) * 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
US8180421B2 (en) 2007-12-12 2012-05-15 Kimberly-Clark Worldwide, Inc. Resonance energy transfer based detection of nosocomial infection
US8619257B2 (en) 2007-12-13 2013-12-31 Kimberley-Clark Worldwide, Inc. Recombinant bacteriophage for detection of nosocomial infection
EP2223086B2 (de) 2007-12-14 2016-06-01 Biotium Inc. Fluoreszenzverbindungen - markierungsfarbstoffe
EP2078750B1 (de) 2008-01-09 2016-09-28 Ludwig-Maximilians-Universität München Fluoreszenter Zwei-Hybrid(F2H)-Assay zur direkten Sichtbarmachung von Proteininteraktionen in lebenden Zellen
GB0800938D0 (en) * 2008-01-18 2008-02-27 Ge Healthcare Uk Ltd Multiplex cell signalling assay
NZ586957A (en) * 2008-02-01 2014-03-28 Chromocell Corp Cell lines and methods for making and using them
EP2254602B1 (de) 2008-02-13 2018-11-21 President and Fellows of Harvard College Kontinuierliche zellprogrammiervorrichtungen
US9370558B2 (en) 2008-02-13 2016-06-21 President And Fellows Of Harvard College Controlled delivery of TLR agonists in structural polymeric devices
US20090236541A1 (en) * 2008-03-24 2009-09-24 General Electric Company System and Methods for Optical Imaging
JP2011516520A (ja) 2008-04-07 2011-05-26 アブリンクス エン.ヴェー. Notch経路に指向性を有するアミノ酸配列及びその使用
EP2268825A4 (de) 2008-04-09 2012-05-02 Cobalt Technologies Inc Immobilisierte produkttolerante mikroorganismen
WO2009146456A1 (en) * 2008-05-30 2009-12-03 President And Fellows Of Harvard College Controlled release of growth factors and signaling molecules for promoting angiogenesis
US20100017232A1 (en) * 2008-07-18 2010-01-21 StevenDale Software, LLC Information Transmittal And Notification System
CN102300583A (zh) * 2008-07-22 2011-12-28 菲布他丁有限合伙公司 Goodpasture抗原结合蛋白及其检测
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
WO2010068747A1 (en) 2008-12-12 2010-06-17 University Of Florida Research Foundation, Inc. Cell-based detection of apf through its interaction with ckap4 for diagnosis of interstitial cystitis
AU2009329501B2 (en) 2008-12-19 2015-11-26 Ablynx N.V. Genetic immunization for producing immunoglobulins against cell-associated antigens such as P2X7, CXCR7 or CXCR4
WO2010107639A2 (en) 2009-03-20 2010-09-23 Board Of Regents, The University Of Texas System Isolation and characterization of novel green fluorescent proteins from copepods
CN105399828B (zh) 2009-04-10 2021-01-15 埃博灵克斯股份有限公司 用于治疗il-6r相关疾病和病症的改进的针对il-6r的氨基酸序列和包含其的多肽
US9297005B2 (en) 2009-04-13 2016-03-29 President And Fellows Of Harvard College Harnessing cell dynamics to engineer materials
CN102724993B (zh) 2009-04-17 2016-04-27 纽约大学 靶向tnf家族受体并拮抗tnf作用的肽、组合物、方法及其用途
EP2438087B1 (de) 2009-06-05 2017-05-10 Ablynx N.V. Trivalente anti-humanes respiratory syncytial virus (hrsv) nanobody konstrukte zur präventionund/oder behandlung von infektionen des atmungstraktes
WO2010151706A1 (en) * 2009-06-26 2010-12-29 Cobalt Technologies, Inc. Integrated system and process for bioproduct production
EP2275442A1 (de) 2009-07-06 2011-01-19 Ludwig-Maximilians-Universität München Erkennung und Visualisierung des Zellzyklus von lebenden Zellen
US8728456B2 (en) 2009-07-31 2014-05-20 President And Fellows Of Harvard College Programming of cells for tolerogenic therapies
US8815779B2 (en) 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
US8451524B2 (en) * 2009-09-29 2013-05-28 Amnis Corporation Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile
US8658434B2 (en) * 2009-10-28 2014-02-25 Biotium, Inc. Fluorescent pyrene compounds
US9644022B2 (en) 2009-11-30 2017-05-09 Ablynx N.V. Amino acid sequences directed against human respiratory syncytial virus (HRSV) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections
US9145447B2 (en) * 2009-12-04 2015-09-29 Allele Biotechnology & Pharmaceuticals, Inc. Photoconvertible fluorescent proteins
WO2011083141A2 (en) 2010-01-08 2011-07-14 Ablynx Nv Method for generation of immunoglobulin sequences by using lipoprotein particles
DE102010009445A1 (de) 2010-02-25 2011-08-25 Universitätsklinikum Jena, 07743 Expressionsvektor zur Enzym-Expression in Zellen und Verfahren zu dessen Verwendung
WO2011109834A2 (en) 2010-03-05 2011-09-09 President And Fellows Of Harvard College Enhancement of skeletal muscle stem cell engrafment by dual delivery of vegf and igf-1
US8817115B1 (en) 2010-05-05 2014-08-26 Amnis Corporation Spatial alignment of image data from a multichannel detector using a reference image
JP5926242B2 (ja) 2010-05-10 2016-05-25 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア エンドリボヌクレアーゼ組成物およびその使用方法
WO2011163669A2 (en) 2010-06-25 2011-12-29 President And Fellows Of Harvard College Co-delivery of stimulatory and inhibitory factors to create temporally stable and spatially restricted zones
EP3202415B1 (de) 2010-06-30 2021-12-08 Compugen Ltd. Fusionsprotein beinhaltend c1orf32 und eine oder mehrere domänen einer immunoglobulin heavy chain konstanten region zur behandlung von multipler sklerose, rheumatoider arthritis und anderen autoimmunerkrankungen
KR20130100290A (ko) 2010-08-30 2013-09-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 협착성 병변을 위한 전단 제어 방출 및 혈전 용해 치료법
WO2012035518A1 (en) 2010-09-17 2012-03-22 Compugen Ltd. Compositions and methods for treatment of drug resistant multiple myeloma
PL2624873T3 (pl) 2010-10-06 2020-12-14 President And Fellows Of Harvard College Nadające się do wstrzykiwania hydrożele tworzące pory do terapii komórkowych opartych na materiałach
US9603894B2 (en) 2010-11-08 2017-03-28 President And Fellows Of Harvard College Materials presenting notch signaling molecules to control cell behavior
CN103403030A (zh) 2011-02-21 2013-11-20 菲布他丁有限合伙公司 治疗和诊断疾病的方法
CN103305546B (zh) * 2011-04-20 2014-08-27 上海交通大学 pGreen Max1 真核表达载体及其制备方法
US10647959B2 (en) 2011-04-27 2020-05-12 President And Fellows Of Harvard College Cell-friendly inverse opal hydrogels for cell encapsulation, drug and protein delivery, and functional nanoparticle encapsulation
WO2012149358A1 (en) 2011-04-28 2012-11-01 President And Fellows Of Harvard College Injectable preformed macroscopic 3-dimensional scaffolds for minimally invasive administration
US9675561B2 (en) 2011-04-28 2017-06-13 President And Fellows Of Harvard College Injectable cryogel vaccine devices and methods of use thereof
US9359363B2 (en) 2011-05-20 2016-06-07 Aquinnah Pharmaceuticals, Inc. Identification of compounds that disperse TDP-43 inclusions
CA2838125A1 (en) 2011-06-03 2012-12-06 President And Fellows Of Harvard College In situ antigen-generating cancer vaccine
SG11201400073SA (en) 2011-08-23 2014-03-28 Harvard College Peptide nanoparticles and uses thereof
GB201121565D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Engineering fluorescent proteins for enhanced fret and uses thereof
GB201121570D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Methods for producing engineered fluorescent proteins for enhanced fret, products and uses thereof
JP6406581B2 (ja) 2011-12-16 2018-10-17 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー オプシンポリペプチドおよびその使用法
EP4108782B1 (de) 2011-12-22 2023-06-07 President and Fellows of Harvard College Zusammensetzungen und verfahren zur analytdetektion
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
WO2013130808A1 (en) 2012-02-29 2013-09-06 D.E. Shaw Research, Llc Methods for screening voltage gated proteins
WO2013138522A2 (en) 2012-03-16 2013-09-19 Genelux Corporation Methods for assessing effectiveness and monitoring oncolytic virus treatment
PT2838515T (pt) 2012-04-16 2020-02-25 Harvard College Composições de sílica mesoporosa para modular respostas imunológicas
WO2013158265A1 (en) 2012-04-20 2013-10-24 Genelux Corporation Imaging methods for oncolytic virus therapy
US9040248B2 (en) 2012-06-06 2015-05-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Kits for detecting and monitoring endocrine disrupting chemicals (EDCs)
CN104684584A (zh) 2012-07-02 2015-06-03 费卜卢斯坦丁公司 Gpbp-1的抑制及其治疗用途
EP2685260A1 (de) 2012-07-09 2014-01-15 Ludwig-Maximilians-Universität München Direkte und quantitative Erkennung von Zielen in lebenden Zellen
EP3539563A1 (de) 2012-07-19 2019-09-18 Redwood Bioscience, Inc. Für cd22 spezifischer antikörper und verfahren zur verwendung davon
US10221221B2 (en) 2012-07-24 2019-03-05 Allele Biotechnology & Pharmaceuticals, Inc Monomeric yellow-green fluorescent protein from cephalochordate
KR102076384B1 (ko) 2012-08-16 2020-02-11 아이피어리언 인코포레이티드 타우병증을 치료하는 방법
CA3124553A1 (en) 2012-10-25 2014-05-01 Bioverativ Usa Inc. Anti-complement c1s antibodies and uses thereof
PT2914291T (pt) 2012-11-02 2022-05-05 Bioverativ Usa Inc Anticorpos anti-complemento c1s e suas utilizações
US9260752B1 (en) 2013-03-14 2016-02-16 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
PL3007726T3 (pl) 2013-06-10 2021-01-11 Ipierian, Inc. Metody leczenia tauopatii
US20160289309A1 (en) 2013-11-27 2016-10-06 Ipierian, Inc. Methods of treating a tauopathy
US9682123B2 (en) 2013-12-20 2017-06-20 The Trustees Of Columbia University In The City Of New York Methods of treating metabolic disease
CA2945945C (en) 2014-04-15 2023-10-03 The Regents Of The University Of California Bi-terminal pegylated integrin-binding peptides and methods of use thereof
WO2015168379A2 (en) 2014-04-30 2015-11-05 President And Fellows Of Harvard College Combination vaccine devices and methods of killing cancer cells
US10577627B2 (en) 2014-06-09 2020-03-03 Voyager Therapeutics, Inc. Chimeric capsids
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
WO2016007886A1 (en) 2014-07-11 2016-01-14 Northwestern University Yeast-based biosensor
CN112553229A (zh) 2014-11-05 2021-03-26 沃雅戈治疗公司 用于治疗帕金森病的aadc多核苷酸
US20170303817A1 (en) 2014-11-12 2017-10-26 Georgia State University Research Foundation, Inc. Surgical articles and methods for detection
SG11201703281RA (en) 2014-11-14 2017-05-30 Voyager Therapeutics Inc Compositions and methods of treating amyotrophic lateral sclerosis (als)
EP3907287A1 (de) 2014-11-14 2021-11-10 Voyager Therapeutics, Inc. Modulatorische polynukleotide
US20170360726A1 (en) 2014-12-05 2017-12-21 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use
CA2969699A1 (en) 2014-12-05 2016-06-09 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use
WO2016094783A1 (en) 2014-12-12 2016-06-16 Voyager Therapeutics, Inc. Compositions and methods for the production of scaav
US20180021364A1 (en) 2015-01-16 2018-01-25 Voyager Therapeutics, Inc. Central nervous system targeting polynucleotides
US11786457B2 (en) 2015-01-30 2023-10-17 President And Fellows Of Harvard College Peritumoral and intratumoral materials for cancer therapy
JP7094533B2 (ja) 2015-04-10 2022-07-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 免疫細胞捕捉デバイスおよびその製造および使用方法
WO2016186948A1 (en) 2015-05-15 2016-11-24 Albert Einstein College Of Medicine, Inc. Gfp-derived fusion tags for protein expression
DK3322733T3 (da) 2015-07-13 2021-10-18 Compugen Ltd Hide1-sammensætninger og fremgangsmåder
US20180180595A1 (en) * 2015-07-24 2018-06-28 The Regents Of The University Of California Cell lines mutant for patched 1 and patched 2 and methods of use thereof
EP3344654B1 (de) 2015-09-02 2020-10-21 Immutep S.A.S. Anti-lag-3-antikörper
AU2016321298A1 (en) 2015-09-09 2018-03-29 The Trustees Of Columbia University In The City Of New York Reduction of ER-MAM localized APP-C99 and methods of treating Alzheimer's Disease
WO2017066705A1 (en) 2015-10-14 2017-04-20 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use against stress granules
WO2017064657A1 (en) 2015-10-16 2017-04-20 Invictus Oncology Pvt. Ltd. Fluorescent anticancer platinum drugs
EP3368054A4 (de) 2015-10-28 2019-07-03 Voyager Therapeutics, Inc. Regulierbare expression unter verwendung des adeno-assoziierten virus (aav)
WO2017096162A1 (en) 2015-12-02 2017-06-08 Voyager Therapeutics, Inc. Assays for the detection of aav neutralizing antibodies
JP7138864B2 (ja) 2016-02-06 2022-09-20 プレジデント アンド フェローズ オブ ハーバード カレッジ 免疫を再構成するための造血ニッチの再現
EP3448874A4 (de) 2016-04-29 2020-04-22 Voyager Therapeutics, Inc. Zusammensetzungen zur behandlung einer erkrankung
US11299751B2 (en) 2016-04-29 2022-04-12 Voyager Therapeutics, Inc. Compositions for the treatment of disease
IL262784B2 (en) 2016-05-18 2023-10-01 Voyager Therapeutics Inc modulatory polynucleotides
EP3484448A4 (de) 2016-07-13 2020-04-01 President and Fellows of Harvard College Antigenpräsentierende zellmimetische gerüste und verfahren zur herstellung und verwendung davon
US11298041B2 (en) 2016-08-30 2022-04-12 The Regents Of The University Of California Methods for biomedical targeting and delivery and devices and systems for practicing the same
JP7222888B2 (ja) 2016-11-16 2023-02-15 アブリンクス エン.ヴェー. Cd123及びtcrアルファ/ベータに結合することが可能なt細胞動員ポリペプチド
CN110913866A (zh) 2017-05-05 2020-03-24 沃雅戈治疗公司 治疗肌萎缩性侧索硬化(als)的组合物和方法
EP3619308A4 (de) 2017-05-05 2021-01-27 Voyager Therapeutics, Inc. Zusammensetzungen und verfahren zur behandlung von morbus huntington
US11497576B2 (en) 2017-07-17 2022-11-15 Voyager Therapeutics, Inc. Trajectory array guide system
EP3662060A2 (de) 2017-08-03 2020-06-10 Voyager Therapeutics, Inc. Zusammensetzungen und verfahren zur aav-freisetzung
CN111479924A (zh) 2017-10-16 2020-07-31 沃雅戈治疗公司 肌萎缩性侧索硬化症(als)的治疗
US20210236644A1 (en) 2017-11-10 2021-08-05 Cocoon Biotech Inc. Ocular applications of silk-based products
WO2019162521A1 (en) 2018-02-26 2019-08-29 Ablynx Nv Improved nucleotide sequences encoding peptide linkers
WO2019173434A1 (en) 2018-03-06 2019-09-12 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes
JP2021521852A (ja) 2018-04-27 2021-08-30 ボイジャー セラピューティクス インコーポレイテッドVoyager Therapeutics,Inc. Aadcウイルスベクターの効力を測定する方法
WO2019222329A1 (en) 2018-05-15 2019-11-21 Voyager Therapeutics, Inc. Compositions and methods for delivery of aav
CN112424359A (zh) 2018-05-15 2021-02-26 沃雅戈治疗公司 用于治疗帕金森氏病的组合物和方法
US20210207167A1 (en) 2018-05-16 2021-07-08 Voyager Therapeutics, Inc. Aav serotypes for brain specific payload delivery
US20210214749A1 (en) 2018-05-16 2021-07-15 Voyager Therapeutics, Inc. Directed evolution
JP2022500066A (ja) 2018-09-28 2022-01-04 ボイジャー セラピューティクス インコーポレイテッドVoyager Therapeutics, Inc. 操作されたプロモータを有するフラタキシン発現コンストラクトおよびその使用方法
GB201817321D0 (en) 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
WO2020227515A1 (en) 2019-05-07 2020-11-12 Voyager Therapeutics, Inc. Compositions and methods for the vectored augmentation of protein destruction, expression and/or regulation
WO2021035325A1 (pt) 2019-08-27 2021-03-04 Fundação Oswaldo Cruz Receptáculo proteico, polinucleotideo, vetor, cassete de expressão, célula, método para produção do receptáculo, método de identificação de patógenos ou de diagnóstico de doenças, uso do receptáculo, e, kit diagnóstico
US20220333133A1 (en) 2019-09-03 2022-10-20 Voyager Therapeutics, Inc. Vectorized editing of nucleic acids to correct overt mutations
EP4135841A1 (de) 2020-04-15 2023-02-22 Voyager Therapeutics, Inc. Tau-bindende verbindungen
AU2021256894A1 (en) 2020-04-17 2022-11-03 Regeneron Pharmaceuticals, Inc. Detection assays for coronavirus neutralizing antibodies
TW202208397A (zh) 2020-05-13 2022-03-01 美商航海家醫療公司 Aav蛋白殼之趨性重定向
WO2022026409A1 (en) 2020-07-27 2022-02-03 Voyager Therapeutics, Inc. Compositions and methods for the treatment of neurological disorders related to glucosylceramidase beta deficiency
US20230285596A1 (en) 2020-07-27 2023-09-14 Voyager Therapeutics, Inc Compositions and methods for the treatment of niemann-pick type c1 disease
WO2023044483A2 (en) 2021-09-20 2023-03-23 Voyager Therapeutics, Inc. Compositions and methods for the treatment of her2 positive cancer
WO2023091949A2 (en) 2021-11-17 2023-05-25 Voyager Therapeutics, Inc. Compositions and methods for the treatment of neurological disorders related to glucosylceramidase beta deficiency
CN116143893B (zh) * 2022-08-09 2023-11-10 无锡佰翱得生物科学股份有限公司 一种增强型单体StayGold蛋白及其应用
WO2024059739A1 (en) 2022-09-15 2024-03-21 Voyager Therapeutics, Inc. Tau binding compounds

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL60888A (en) 1978-06-16 1984-07-31 Yeda Res & Dev Substrates and process for the quantitative assay of enzymes
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5070012A (en) 1988-03-30 1991-12-03 The Board Of Trustees Of The Leland Stanford Junior University Monitoring of cells and trans-activating transcription elements
US5134232A (en) 1988-11-14 1992-07-28 The Regents Of The University Of California Fluorescent indicator dyes for alkali metal cations
GB8916806D0 (en) 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
EP0428000A1 (de) 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
EP0537270B1 (de) 1990-07-02 1998-09-09 The Regents Of The University Of California Bestimmung von analyten durch übertragung von fluoreszenzenergie
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
DE4105354A1 (de) * 1991-02-21 1992-08-27 Basf Ag Waessrige polymerdispersionen, enthaltend organische verbindungen mit carbonat- und carbonylgruppen, sowie damit hergestellte anstrich- und beschichtungssysteme
GB9310978D0 (en) 1993-05-27 1993-07-14 Zeneca Ltd Compounds
EP0759170B1 (de) 1993-09-10 2008-07-09 The Trustees Of Columbia University In The City Of New York Verwendung von grünem fluoreszenzprotein
US5491084A (en) 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995021191A1 (en) * 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
DE69618350T2 (de) 1995-01-31 2002-09-19 Bioimage As Soeborg Ein verfahren zum nachweis biologisch aktiver substanzen
WO1996027027A1 (en) 1995-03-02 1996-09-06 Rutgers The State University Of New Jersey Improved method for purifying green fluorescent protein
GB9504446D0 (en) 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
DE69604298T2 (de) 1995-09-22 2000-05-18 Bioimage A S Soeborg Varianten des grünen fluoreszenzproteins, gfp
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6140132A (en) * 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample

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EP0804457B1 (de) 2001-06-06
US20020123113A1 (en) 2002-09-05
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PT804457E (pt) 2001-09-28
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US6066476A (en) 2000-05-23

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