DE69431285T2 - Herstellungsmethode von markierten genen; transcripten und proteinen - Google Patents

Herstellungsmethode von markierten genen; transcripten und proteinen

Info

Publication number
DE69431285T2
DE69431285T2 DE69431285T DE69431285T DE69431285T2 DE 69431285 T2 DE69431285 T2 DE 69431285T2 DE 69431285 T DE69431285 T DE 69431285T DE 69431285 T DE69431285 T DE 69431285T DE 69431285 T2 DE69431285 T2 DE 69431285T2
Authority
DE
Germany
Prior art keywords
transcripts
proteins
production method
marked genes
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69431285T
Other languages
English (en)
Other versions
DE69431285D1 (de
Inventor
Jonathan Wallace Jarvik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of DE69431285D1 publication Critical patent/DE69431285D1/de
Application granted granted Critical
Publication of DE69431285T2 publication Critical patent/DE69431285T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
DE69431285T 1993-01-05 1994-01-05 Herstellungsmethode von markierten genen; transcripten und proteinen Expired - Fee Related DE69431285T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/000,619 US5652128A (en) 1993-01-05 1993-01-05 Method for producing tagged genes, transcripts, and proteins
PCT/US1994/000254 WO1994016092A1 (en) 1993-01-05 1994-01-05 Method for producing tagged genes, transcripts, and proteins

Publications (2)

Publication Number Publication Date
DE69431285D1 DE69431285D1 (de) 2002-10-10
DE69431285T2 true DE69431285T2 (de) 2003-07-31

Family

ID=21692289

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69431285T Expired - Fee Related DE69431285T2 (de) 1993-01-05 1994-01-05 Herstellungsmethode von markierten genen; transcripten und proteinen

Country Status (5)

Country Link
US (5) US5652128A (de)
EP (1) EP0656954B1 (de)
DE (1) DE69431285T2 (de)
ES (1) ES2181714T3 (de)
WO (1) WO1994016092A1 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5652128A (en) * 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5789655A (en) * 1994-05-13 1998-08-04 The Regents Of The University Of California Transgenic animals expressing artificial epitope-tagged proteins
AUPN480095A0 (en) * 1995-08-15 1995-09-07 Commonwealth Scientific And Industrial Research Organisation Epitope tagging system
US5866330A (en) * 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5695937A (en) * 1995-09-12 1997-12-09 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US6346392B1 (en) * 1996-11-27 2002-02-12 Smithkline Beecham Corporation Polynucleotides encoding a novel glutamine transport ATP-binding protein
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
AU5168598A (en) * 1996-11-08 1998-05-29 Jonathan W. Jarvik Method for producing tagged genes, transcripts and proteins
US5948677A (en) * 1996-12-09 1999-09-07 Jarvik; Jonathan W. Reading frame independent epitope tagging
CA2205888A1 (en) 1997-07-11 1999-01-11 John J. Priatel Complementation trap
CA2224475A1 (en) * 1998-02-25 1999-08-25 University Of British Columbia Protein interaction and transcription factor trap
CA2263784A1 (en) 1998-03-23 1999-09-23 Megabios Corporation Dual-tagged proteins and their uses
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6498286B1 (en) 1998-08-20 2002-12-24 Ffr Cooperative Germplasm containing an identifier nucleotide sequence and method for identifying germplasm
EP1165775A2 (de) 1999-03-05 2002-01-02 Maxygen, Inc. Rekombination von insertionsmodifizierten nucleinsäuren
EP1220928A2 (de) * 1999-09-30 2002-07-10 Alexion Pharmaceuticals, Inc. Zusammensetzungen und verfahren zur veränderung der genexpression
US7816098B2 (en) * 2000-01-31 2010-10-19 Sense Proteomic Limited Methods of making and using a protein array
GB2361698B (en) * 2000-01-31 2003-06-25 Sense Proteomic Ltd Modification of DNA molecules
US6897020B2 (en) * 2000-03-20 2005-05-24 Newlink Genetics Inc. Methods and compositions for elucidating relative protein expression levels in cells
US20060134629A1 (en) * 2000-03-20 2006-06-22 Link Charles J Methods and compositions for elucidating protein expression profiles in cells
AU2001259470A1 (en) * 2000-05-05 2001-11-20 Purdue Research Foundation Affinity selected signature peptides for protein identification and quantification
JP2002142765A (ja) * 2000-07-14 2002-05-21 Tosoh Corp 新規ゲノム解析法
US20020137053A1 (en) * 2000-07-19 2002-09-26 Dana Ault-Riche Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
CA2419322C (en) * 2000-08-14 2012-10-23 Donald L. Court Enhanced homologous recombination mediated by lambda recombination proteins
US6821747B2 (en) 2001-07-12 2004-11-23 Automated Cell, Inc. Suppression of non-biological motion
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
WO2003027682A2 (en) * 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
US20040048311A1 (en) * 2002-01-24 2004-03-11 Dana Ault-Riche Use of collections of binding sites for sample profiling and other applications
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP1576126A3 (de) * 2002-10-30 2005-10-26 Pointilliste, Inc. Systeme zum einfangen und zur analyse biologischer partikel und verfahren unter verwendung dieser systeme
AU2003290561A1 (en) * 2003-02-10 2004-09-06 Dana Ault-Riche Self-assembling arrays and uses thereof
US20050037409A1 (en) * 2003-07-29 2005-02-17 Kauvar Lawrence M. Translocation tagging
US7365179B2 (en) * 2003-09-09 2008-04-29 Compass Genetics, Llc Multiplexed analytical platform
WO2005029040A2 (en) * 2003-09-18 2005-03-31 Parallele Biosciences, Inc. System and methods for enhancing signal-to-noise ratios of microarray-based measurements
CA2540244A1 (en) * 2003-09-30 2005-04-14 Lexicon Genetics Incorporated Methods and compositions for defining gene function
US20050095648A1 (en) * 2003-10-30 2005-05-05 Mario Geysen Method for designing linear epitopes and algorithm therefor and polypeptide epitopes
WO2005067980A2 (en) * 2004-01-12 2005-07-28 Pointilliste, Inc. Design of therapeutics and therapeutics
CA2555377A1 (en) * 2004-02-12 2005-09-01 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
JP2008507294A (ja) 2004-07-26 2008-03-13 ダウ グローバル テクノロジーズ インコーポレイティド 菌株遺伝子操作による改善されたタンパク質発現のための方法
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
EP1856293A2 (de) * 2005-03-16 2007-11-21 Compass Genetics, Llc Verfahren und zusammensetzungen zum ablesen von assays auf mehrfachen analytischen plattformen
NZ565711A (en) 2005-08-09 2011-10-28 Revivicor Inc Transgenic ungulates expressing a porcine CTLA4 peptide fused to an immunoglobulin
EP1931787A4 (de) * 2005-09-30 2010-10-20 Univ Montana State System zum nachweis von protein-protein-wechselwirkungen
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
BRPI0810120A2 (pt) 2007-04-27 2014-11-11 Dow Global Technologies Inc Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas
US20100298166A1 (en) * 2008-01-24 2010-11-25 Uri Alon Cell populations for polypeptide analysis and uses of same
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0451700A1 (de) * 1990-04-10 1991-10-16 Miles Inc. Rekombinante APP Minigene zur Expression in Transgen-Mäusen als Alzheimer-Krankheitsmuster
DE4021458C1 (de) * 1990-07-05 1991-08-29 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften Ev, 3400 Goettingen, De
WO1992013071A1 (en) * 1991-01-28 1992-08-06 Massachusetts Institute Of Technology Method of exon amplification
US5652128A (en) * 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins

Also Published As

Publication number Publication date
US6080541A (en) 2000-06-27
US5916810A (en) 1999-06-29
EP0656954A1 (de) 1995-06-14
WO1994016092A1 (en) 1994-07-21
US6096717A (en) 2000-08-01
DE69431285D1 (de) 2002-10-10
EP0656954A4 (de) 1997-04-09
EP0656954B1 (de) 2002-09-04
ES2181714T3 (es) 2003-03-01
US6472207B1 (en) 2002-10-29
US5652128A (en) 1997-07-29

Similar Documents

Publication Publication Date Title
DE69431285T2 (de) Herstellungsmethode von markierten genen; transcripten und proteinen
ATE251627T1 (de) Verfahren und kristallformen von 2- methylthienobenzodiazepinen
DE69435262D1 (de) Herstellungsverfahren von Elektroden
DE69629438D1 (de) Mischungen von absorbierbaren Polyoxaestern die Amin und/oder Amido-Gruppen enthalten
DE69431408D1 (de) Herstellung von polysuccinimid und polyasparaginsäure
KR950700990A (ko) 신규의 분비단백질 에프-스폰딘의 클로닝, 발현 및 용도
DE69725326D1 (de) Herstellung von fugizides chinazolinonen und nützliche zwischenprodukte
FI945636A0 (fi) Menetelmä laktidin puhdistamiseksi
DE69427919D1 (de) Doppelrilliger retroreflektiver würfeleckiger körper und herstellungsverfahren
DE59606486D1 (de) Herstellung von Poly-o-hydroxyamiden und Poly-o-mercaptoamiden
DE69425131T2 (de) Verwendung von clavulansäure und einem antibiotikum zur behandlung von infektionen
DE69218129D1 (de) Modifiziertes Xanthangummi und Verfahren zur Modifizierung von Xanthangummi
DE69534624D1 (de) Gentranscripte von MHC Klasse I HLA-G und deren Verwendungen
DE59609208D1 (de) Herstellung von Mischkristallen und festen Lösungen von 1,4-Diketopyrrolopyrrolen
DE69312205D1 (de) Reinigungsmethode von Rohprodukt
DE69434562D1 (de) Spannungsgesteuerter Thyristor und Verfahren zu seiner Herstellung
DE69630038D1 (de) Verfahren zur herstellung und aufreinigung von enzymen
DE59405004D1 (de) Herstellung von 2-H-Heptafluorpropan
DE68924258T2 (de) Verfahren zur herstellung von n-(hydroxyalkyl)-2-ungesättigten amiden und 2-alkenyloxazolinen.
DE69529760T2 (de) Riemenkonstruktion und herstellungsverfahren dieser riemenkonstruktion
DE69321885T2 (de) Verfahren zur fermentativen Herstellung von 10-Hydroxy-C18-Carbonsäure und Gamma-Dodekalaktonderivaten
DE69717846T2 (de) Behandlungsverfahren von laktamen
DE59604429D1 (de) Herstellung von difluorchlor- und dichloracetylchlorid
DE58905768D1 (de) Verfahren zur herstellung von zur rektalen und vaginalen anwendung geeigneten praeparaten biologisch aktiver peptide.
DE69213637T2 (de) Herstellung von 2-Hydroxymandelsäuren und 2-Hydroxybenzaldehyden

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee