DE69404018D1 - Verfahren zur Herstellung von Polynukleotiden auf einem Festträger und Apparat für dieses Verfahren - Google Patents

Verfahren zur Herstellung von Polynukleotiden auf einem Festträger und Apparat für dieses Verfahren

Info

Publication number
DE69404018D1
DE69404018D1 DE69404018T DE69404018T DE69404018D1 DE 69404018 D1 DE69404018 D1 DE 69404018D1 DE 69404018 T DE69404018 T DE 69404018T DE 69404018 T DE69404018 T DE 69404018T DE 69404018 D1 DE69404018 D1 DE 69404018D1
Authority
DE
Germany
Prior art keywords
solid support
reactor
column
solutions
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69404018T
Other languages
English (en)
Other versions
DE69404018T2 (de
Inventor
Francois Chatelain
Viktor Kumarev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Serono Genetics Institute SA
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serono Genetics Institute SA, Genset SA filed Critical Serono Genetics Institute SA
Publication of DE69404018D1 publication Critical patent/DE69404018D1/de
Application granted granted Critical
Publication of DE69404018T2 publication Critical patent/DE69404018T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00308—Reactor vessels in a multiple arrangement interchangeably mounted in racks or blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00324—Reactor vessels in a multiple arrangement the reactor vessels or wells being arranged in plates moving in parallel to each other
    • B01J2219/00328—Movement by linear translation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/00409—Solenoids in combination with valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
DE69404018T 1993-12-16 1994-12-14 Verfahren zur Herstellung von Polynukleotiden auf einem Festträger und Apparat für dieses Verfahren Expired - Lifetime DE69404018T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9315164A FR2714061B1 (fr) 1993-12-16 1993-12-16 Procédé de préparation de polynucléotides sur support solide et appareil permettant sa mise en Óoeuvre.

Publications (2)

Publication Number Publication Date
DE69404018D1 true DE69404018D1 (de) 1997-08-07
DE69404018T2 DE69404018T2 (de) 1998-01-15

Family

ID=9454019

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69404018T Expired - Lifetime DE69404018T2 (de) 1993-12-16 1994-12-14 Verfahren zur Herstellung von Polynukleotiden auf einem Festträger und Apparat für dieses Verfahren

Country Status (14)

Country Link
US (3) US5869643A (de)
EP (1) EP0658566B1 (de)
JP (1) JPH08239397A (de)
KR (1) KR100366862B1 (de)
AT (1) ATE154936T1 (de)
AU (1) AU693487B2 (de)
CA (1) CA2138240C (de)
DE (1) DE69404018T2 (de)
DK (1) DK0658566T3 (de)
ES (1) ES2106479T3 (de)
FR (1) FR2714061B1 (de)
GR (1) GR3024829T3 (de)
HK (1) HK1002330A1 (de)
TW (1) TW349101B (de)

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EP0695941B1 (de) * 1994-06-08 2002-07-31 Affymetrix, Inc. Verfahren und Vorrichtung zum Verpacken von Chips
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DE19602464B4 (de) * 1996-01-24 2006-05-04 Rapp, Wolfgang, Dr. Vorrichtung zur multiplen, gleichzeitigen und parallelen Synthese chemischer Verbindungen und zur diskreten Weiterbehandlung von Aliquoten
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US6864092B1 (en) * 1998-08-13 2005-03-08 Symyx Technologies, Inc. Parallel reactor with internal sensing and method of using same
US6528026B2 (en) 1998-08-13 2003-03-04 Symyx Technologies, Inc. Multi-temperature modular reactor and method of using same
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US6451998B1 (en) 1999-10-18 2002-09-17 Agilent Technologies, Inc. Capping and de-capping during oligonucleotide synthesis
EP1310296A3 (de) * 2000-06-03 2006-02-01 Symyx Technologies, Inc. Semikontinuierliche oder kontinuierliche Parallelreaktoren mit Einspeisungsdruckbeaufschlagungsvorrichtung
US6994827B2 (en) * 2000-06-03 2006-02-07 Symyx Technologies, Inc. Parallel semicontinuous or continuous reactors
US20040215010A1 (en) * 2000-06-13 2004-10-28 Viktor Kumarev Universal solid supports for solid phase oligosynthesis and methods for their preparation and use
US20060141507A1 (en) * 2000-09-27 2006-06-29 Kronick Mel N Manufacture and use of non-standard size microarray slides
WO2002099003A2 (en) * 2001-06-06 2002-12-12 Monsanto Technology Llc Parallel reactor system and method
US6761855B1 (en) 2001-11-05 2004-07-13 Biosearch Technologies, Inc. Column for solid phase processing
US20040082058A1 (en) * 2002-10-29 2004-04-29 Arthur Schleifer Array hybridization apparatus and method for making uniform sample volumes
US20040086868A1 (en) * 2002-10-30 2004-05-06 Parker Russell A. Enclosed arrays and their reading
US7247337B1 (en) 2002-12-16 2007-07-24 Agilent Technologies, Inc. Method and apparatus for microarray fabrication
US20040214310A1 (en) * 2003-04-25 2004-10-28 Parker Russell A. Apparatus and method for array alignment
US20040223890A1 (en) * 2003-05-09 2004-11-11 Summers Douglas G. Clamshell slide holder
US20050026304A1 (en) * 2003-07-30 2005-02-03 Laurakay Bruhn Microarray based affinity purification and analysis device coupled with secondary analysis technologies
EP1694428A4 (de) * 2003-12-15 2010-10-06 Life Technologies Corp Automatisierte oligomersynthese
DE10361003B3 (de) * 2003-12-23 2005-07-28 Hte Ag The High Throughput Experimentation Company Vorrichtung und Verfahren zur Druck- und Flusskontrolle in Parallelreaktoren
US20050196761A1 (en) * 2004-03-08 2005-09-08 Thompson Allen C. Array hybridization apparatus and method
US20050214930A1 (en) * 2004-03-26 2005-09-29 Bynum Magdalena A Apparatus and method for preventing cross contamination when dissassembling an array
US20050227239A1 (en) * 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
US7560417B2 (en) * 2005-01-13 2009-07-14 Wisconsin Alumni Research Foundation Method and apparatus for parallel synthesis of chain molecules such as DNA
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
WO2007022110A2 (en) * 2005-08-12 2007-02-22 Symyx Technologies, Inc. Event-based library process design
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US20070105125A1 (en) * 2005-11-08 2007-05-10 Tsai George P Chemical array housing having a gas delivery element and methods of using the same
US8309710B2 (en) * 2009-06-29 2012-11-13 Agilent Technologies, Inc. Use of N-alkyl imidazole for sulfurization of oligonucleotides with an acetyl disulfide
US8642755B2 (en) * 2009-06-30 2014-02-04 Agilent Technologies, Inc. Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
US9701708B2 (en) 2013-01-31 2017-07-11 Ionis Pharmaceuticals, Inc. Method of preparing oligomeric compounds using modified coupling protocols
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Also Published As

Publication number Publication date
JPH08239397A (ja) 1996-09-17
ATE154936T1 (de) 1997-07-15
HK1002330A1 (en) 1998-08-14
KR950018035A (ko) 1995-07-22
TW349101B (en) 1999-01-01
AU693487B2 (en) 1998-07-02
ES2106479T3 (es) 1997-11-01
DK0658566T3 (da) 1998-02-02
US6825339B2 (en) 2004-11-30
US20030176690A1 (en) 2003-09-18
CA2138240C (fr) 2005-04-05
EP0658566A1 (de) 1995-06-21
KR100366862B1 (ko) 2003-12-01
EP0658566B1 (de) 1997-07-02
AU8043294A (en) 1995-06-22
US5869643A (en) 1999-02-09
US6623703B1 (en) 2003-09-23
CA2138240A1 (fr) 1995-06-17
GR3024829T3 (en) 1998-01-30
DE69404018T2 (de) 1998-01-15
FR2714061B1 (fr) 1996-03-08
FR2714061A1 (fr) 1995-06-23

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