DE69227997T2 - Verfahren mit einem gen-sonden biosensor - Google Patents

Verfahren mit einem gen-sonden biosensor

Info

Publication number
DE69227997T2
DE69227997T2 DE69227997T DE69227997T DE69227997T2 DE 69227997 T2 DE69227997 T2 DE 69227997T2 DE 69227997 T DE69227997 T DE 69227997T DE 69227997 T DE69227997 T DE 69227997T DE 69227997 T2 DE69227997 T2 DE 69227997T2
Authority
DE
Germany
Prior art keywords
immobilised
waveguide
wave detector
rna
hybridisation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69227997T
Other languages
English (en)
Other versions
DE69227997D1 (de
Inventor
David Squirrell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of DE69227997D1 publication Critical patent/DE69227997D1/de
Application granted granted Critical
Publication of DE69227997T2 publication Critical patent/DE69227997T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
DE69227997T 1991-09-16 1992-09-16 Verfahren mit einem gen-sonden biosensor Expired - Lifetime DE69227997T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB919119735A GB9119735D0 (en) 1991-09-16 1991-09-16 Gene probe biosensor method
PCT/GB1992/001698 WO1993006241A1 (en) 1991-09-16 1992-09-16 Gene probe biosensor method

Publications (2)

Publication Number Publication Date
DE69227997D1 DE69227997D1 (de) 1999-02-04
DE69227997T2 true DE69227997T2 (de) 1999-05-12

Family

ID=10701461

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69227997T Expired - Lifetime DE69227997T2 (de) 1991-09-16 1992-09-16 Verfahren mit einem gen-sonden biosensor

Country Status (20)

Country Link
US (1) US5750337A (de)
EP (1) EP0604506B1 (de)
JP (1) JP3324607B2 (de)
KR (1) KR100249288B1 (de)
AT (1) ATE174968T1 (de)
AU (1) AU665369B2 (de)
BG (1) BG62337B1 (de)
CA (1) CA2116239C (de)
CZ (1) CZ286927B6 (de)
DE (1) DE69227997T2 (de)
DK (1) DK0604506T3 (de)
ES (1) ES2125272T3 (de)
FI (1) FI108463B (de)
GB (2) GB9119735D0 (de)
GR (1) GR3029722T3 (de)
HU (1) HU218596B (de)
NO (1) NO315380B1 (de)
RU (1) RU2116349C1 (de)
SK (1) SK280713B6 (de)
WO (1) WO1993006241A1 (de)

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WO1993020240A1 (en) * 1992-04-06 1993-10-14 Abbott Laboratories Method and device for detection of nucleic acid or analyte using total internal reflectance
WO1995026416A1 (en) * 1994-03-25 1995-10-05 Research Corporation Technologies, Inc. Nucleic acid biosensor diagnostics
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
EP0795032A2 (de) * 1994-12-22 1997-09-17 Abbott Laboratories Verfahren zur immobilisierung von oligonukleotiden auf festen trägermaterial und verfahren zur anwendung träger gebundener oligonukleotiden
US5690894A (en) * 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
SE9502608D0 (sv) * 1995-07-14 1995-07-14 Pharmacia Biosensor Ab Method for nucleic acid senquencing
WO1997032212A1 (en) * 1996-03-01 1997-09-04 Beckman Instruments, Inc. System for simultaneously conducting multiple ligand binding assays
US5832165A (en) * 1996-08-28 1998-11-03 University Of Utah Research Foundation Composite waveguide for solid phase binding assays
NZ502303A (en) 1997-06-18 2002-02-01 Ulrich J Krull Nucleic acid biosensor system & diagnostic use
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
EP2267165B1 (de) * 1997-07-28 2016-11-30 Gen-Probe Incorporated Sequenzanalyse von Nukleinsäuren
GB2335035B (en) * 1998-03-03 2003-05-28 Brax Genomics Ltd Screening for functional antisense agents
GB9805935D0 (en) * 1998-03-19 1998-05-13 Ciba Geigy Ag Organic compounds
EP0957354A3 (de) * 1998-03-20 2000-03-22 IA Inc. Verfahren und Vorrichtung zum Messen der Bindung zwischen Biomolekülen
US6429023B1 (en) 1998-07-20 2002-08-06 Shayda Technologies, Inc. Biosensors with polymeric optical waveguides
US6699661B1 (en) * 1999-04-20 2004-03-02 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
US7205347B2 (en) * 2000-10-19 2007-04-17 Trans Photonics, Llc. Substituted-polyaryl chromophoric compounds
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6597499B2 (en) 2001-01-25 2003-07-22 Olympus Optical Co., Ltd. Total internal reflection fluorescence microscope having a conventional white-light source
EP1432829A4 (de) * 2001-10-04 2005-01-19 Univ California Nachweis einer polynukleotidhybridisierung
DE102004016247A1 (de) * 2004-04-02 2005-10-20 Chromeon Gmbh Feste Trägersysteme zum homogenen Nachweis von Wechselwirkungen von Biomolekülen ohne Waschschritte
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) * 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
WO2007105786A1 (ja) * 2006-03-16 2007-09-20 National University Corporation Akita University 核酸検出方法及び核酸検出キット
US20080009420A1 (en) * 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
US7824902B2 (en) * 2006-11-03 2010-11-02 Mark Selker Optical interface for disposable bioreactors
EP1935496A1 (de) 2006-12-22 2008-06-25 Eppendorf Array Technologies SA Vorrichtung und/oder Verfahren zur Detektion erweiterter Nukleotidsequenzen auf Mikro-Arrays
CN101589303B (zh) * 2007-01-17 2012-01-11 霍尼韦尔国际公司 基于倏逝波检测的微阵列读取器和读取微阵列的方法
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US9005931B2 (en) 2007-12-24 2015-04-14 Honeywell International Inc. Programmable oligonucleotide micro array
EP2227537A4 (de) * 2007-12-24 2014-05-07 Honeywell Int Inc Reaktor zur quantitativen analyse von nukleinsäuren
RU2508536C2 (ru) 2008-02-04 2014-02-27 Конинклейке Филипс Электроникс Н.В. Система и способ обнаружения свечения
US9539571B2 (en) 2010-01-20 2017-01-10 Honeywell International Inc. Method to increase detection efficiency of real time PCR microarray by quartz material
US20140371082A1 (en) * 2011-06-30 2014-12-18 Bioquanta Sa Process and apparatus for quantifying nucleic acid in a sample
KR102376437B1 (ko) 2016-10-27 2022-03-17 바나디스 다이아그노스틱스 회전환 증폭 생성물을 처리하는 방법
BR112021017166A2 (pt) * 2019-03-01 2021-11-09 Vidya Holdings Ltd Cartucho de ensaio para detectar um componente alvo em um fluido, e, método para fabricar um arranjo de cartuchos de ensaio

Family Cites Families (14)

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Publication number Priority date Publication date Assignee Title
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4582809A (en) * 1982-06-14 1986-04-15 Myron J. Block Apparatus including optical fiber for fluorescence immunoassay
EP0144914A3 (de) * 1983-12-12 1986-08-13 Miles Inc. Hybridisierungstest mittels markierter Paare von hybridbindenden Reagenzien
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5242797A (en) * 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
EP0245206A1 (de) * 1986-05-05 1987-11-11 IntraCel Corporation Analytisches Verfahren zum Nachweis und zur Messung einer spezifisch sequentierten Nukleinsäure
BE1000572A4 (fr) * 1987-05-20 1989-02-07 Block Myron J Cellule rta, appareil et procede pour titrer un polynucleotide dans un liquide.
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
GB8919411D0 (en) * 1989-08-25 1989-10-11 Amersham Int Plc Assay method
CA2032203C (en) * 1989-12-29 2009-05-19 Christine L. Brakel Amplification capture assay
DE69125441T2 (de) * 1990-09-28 1997-11-06 Toshiba Kawasaki Kk Verfahren zum Gennachweis
USH1398H (en) * 1990-12-28 1995-01-03 Campbell; James R. DNA-based fluourescent sensor

Also Published As

Publication number Publication date
DK0604506T3 (da) 1999-08-23
JPH06510670A (ja) 1994-12-01
BG98660A (bg) 1995-03-31
SK30694A3 (en) 1994-07-06
WO1993006241A1 (en) 1993-04-01
CA2116239C (en) 2008-11-18
RU94019336A (ru) 1996-09-27
AU665369B2 (en) 1996-01-04
ATE174968T1 (de) 1999-01-15
GB2274710B (en) 1995-09-06
CZ52194A3 (en) 1994-07-13
SK280713B6 (sk) 2000-06-12
ES2125272T3 (es) 1999-03-01
AU2564192A (en) 1993-04-27
US5750337A (en) 1998-05-12
GB9119735D0 (en) 1991-10-30
DE69227997D1 (de) 1999-02-04
GB2274710A (en) 1994-08-03
KR100249288B1 (ko) 2000-04-01
EP0604506A1 (de) 1994-07-06
RU2116349C1 (ru) 1998-07-27
GR3029722T3 (en) 1999-06-30
BG62337B1 (bg) 1999-08-31
NO315380B1 (no) 2003-08-25
CZ286927B6 (cs) 2000-08-16
JP3324607B2 (ja) 2002-09-17
EP0604506B1 (de) 1998-12-23
NO940901L (no) 1994-05-16
FI941220A0 (fi) 1994-03-15
HUT68771A (en) 1995-07-28
GB9402823D0 (en) 1994-04-27
NO940901D0 (no) 1994-03-14
FI108463B (fi) 2002-01-31
HU9400770D0 (en) 1994-06-28
HU218596B (hu) 2000-10-28
CA2116239A1 (en) 1993-04-01
FI941220A (fi) 1994-03-15

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Legal Events

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