DE69225646T2 - Verfahren zur Immobilisierung eines Nukleinsäurefragments durch passive Fixierung auf einen Festträger, daraus erhaltener Festträger und seine Verwendung - Google Patents
Verfahren zur Immobilisierung eines Nukleinsäurefragments durch passive Fixierung auf einen Festträger, daraus erhaltener Festträger und seine VerwendungInfo
- Publication number
- DE69225646T2 DE69225646T2 DE69225646T DE69225646T DE69225646T2 DE 69225646 T2 DE69225646 T2 DE 69225646T2 DE 69225646 T DE69225646 T DE 69225646T DE 69225646 T DE69225646 T DE 69225646T DE 69225646 T2 DE69225646 T2 DE 69225646T2
- Authority
- DE
- Germany
- Prior art keywords
- solid support
- immobilizing
- nucleic acid
- acid fragment
- obtained therefrom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9109057A FR2679255B1 (fr) | 1991-07-17 | 1991-07-17 | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69225646D1 DE69225646D1 (de) | 1998-07-02 |
DE69225646T2 true DE69225646T2 (de) | 1998-12-24 |
Family
ID=9415243
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69225646T Expired - Lifetime DE69225646T2 (de) | 1991-07-17 | 1992-07-17 | Verfahren zur Immobilisierung eines Nukleinsäurefragments durch passive Fixierung auf einen Festträger, daraus erhaltener Festträger und seine Verwendung |
Country Status (9)
Country | Link |
---|---|
US (1) | US5510084A (de) |
EP (1) | EP0524864B1 (de) |
JP (1) | JP2797047B2 (de) |
AU (1) | AU659176B2 (de) |
CA (1) | CA2074135C (de) |
DE (1) | DE69225646T2 (de) |
ES (1) | ES2118120T3 (de) |
FI (1) | FI102619B1 (de) |
FR (1) | FR2679255B1 (de) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2706618B1 (fr) * | 1993-06-11 | 1995-09-01 | Bio Merieux | Dispositif pour le dosage d'haptènes et son utilisation. |
FR2707010B1 (de) * | 1993-06-25 | 1995-09-29 | Bio Merieux | |
US5656427A (en) * | 1994-08-29 | 1997-08-12 | Gen-Probe Incorporated | Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid |
FR2726277B1 (fr) | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
CA2207629A1 (en) * | 1994-12-22 | 1996-06-27 | Abbott Laboratories | Methods of immobilizing oligonucleotides to solid support materials and methods of using support bound oligonucleotides |
FR2733755B1 (fr) * | 1995-05-03 | 1997-07-11 | Bio Merieux | Fragment nucleotidique de l'arn ribosomique 16s de corynebacteries, sondes et amorces derivees, reactif et procede de detection |
FR2758884B1 (fr) * | 1997-01-30 | 1999-04-02 | Bio Merieux | Procede pour isoler, notamment detecter ou quantifier un analyte dans un milieu |
US6692912B1 (en) | 1997-03-05 | 2004-02-17 | Matrix Technologies Corporation | Nucleic acid-containing polymerizable complex |
US5932711A (en) * | 1997-03-05 | 1999-08-03 | Mosaic Technologies, Inc. | Nucleic acid-containing polymerizable complex |
US6558901B1 (en) * | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
US5786182A (en) * | 1997-05-02 | 1998-07-28 | Biomerieux Vitek, Inc. | Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use |
US6429007B1 (en) | 1997-05-02 | 2002-08-06 | BIOMéRIEUX, INC. | Nucleic acid amplification reaction station for disposable test devices |
US5989499A (en) * | 1997-05-02 | 1999-11-23 | Biomerieux, Inc. | Dual chamber disposable reaction vessel for amplification reactions |
US6410275B1 (en) | 1997-05-02 | 2002-06-25 | Biomerieux, Inc. | Disposable test devices for performing nucleic acid amplification reactions |
IL124275A (en) * | 1997-05-02 | 2002-03-10 | Bio Merieux Vitek Inc | A method to produce sequences of nucleic acids |
US5858653A (en) * | 1997-09-30 | 1999-01-12 | Surmodics, Inc. | Reagent and method for attaching target molecules to a surface |
US6762019B2 (en) | 1997-09-30 | 2004-07-13 | Surmodics, Inc. | Epoxide polymer surfaces |
US6465178B2 (en) * | 1997-09-30 | 2002-10-15 | Surmodics, Inc. | Target molecule attachment to surfaces |
US6703228B1 (en) | 1998-09-25 | 2004-03-09 | Massachusetts Institute Of Technology | Methods and products related to genotyping and DNA analysis |
EP1001037A3 (de) * | 1998-09-28 | 2003-10-01 | Whitehead Institute For Biomedical Research | Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus |
US20050153926A1 (en) * | 1998-12-04 | 2005-07-14 | Adams Christopher P. | Method for the immobilization of oligonucleotides |
CA2353596A1 (en) * | 1998-12-04 | 2000-06-15 | Mosaic Technologies, Inc. | Method for the immobilization of oligonucleotides |
US6383783B1 (en) | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
GB0016836D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
US6706479B2 (en) * | 2000-10-05 | 2004-03-16 | Virginia Tech Intellectual Properties, Inc. | Bio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chips |
JP2005538929A (ja) | 2002-01-16 | 2005-12-22 | ダイナル バイオテック エイエスエイ | 単一サンプルからの核酸及びタンパク質の単離方法 |
US20040067495A1 (en) * | 2002-10-08 | 2004-04-08 | Ray Gustav Allen | Polynucleic acid and polyamino acid binding substrate |
US7309593B2 (en) * | 2003-10-01 | 2007-12-18 | Surmodics, Inc. | Attachment of molecules to surfaces |
US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
US7727710B2 (en) | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
US7939249B2 (en) | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
EP1623996A1 (de) * | 2004-08-06 | 2006-02-08 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek |
WO2007099856A1 (ja) * | 2006-02-23 | 2007-09-07 | National University Corporation Hokkaido University | 質量分析用生体関連分子のエステル化法及び得られたエステル化誘導体の質量分析方法 |
EP2140001A2 (de) * | 2007-04-25 | 2010-01-06 | 3M Innovative Properties Company | Verfahren zur amplifikation von nukleinsäuren |
AU2010225035A1 (en) * | 2009-03-19 | 2011-09-29 | Seps Pharma N.V. | Fosfluconazole derivatives, synthesis, and use in long acting formulations |
EP4341270A1 (de) * | 2021-05-21 | 2024-03-27 | Olix US, Inc. | Chemische phosphorylierungsreagenzien, herstellung und verwendungen davon |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4379843A (en) * | 1981-01-26 | 1983-04-12 | Peter Cashion | Immobilization of polynucleotides and polypeptides with tritylated polysaccharides |
US4994373A (en) * | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
US4948882A (en) * | 1983-02-22 | 1990-08-14 | Syngene, Inc. | Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis |
US4828979A (en) * | 1984-11-08 | 1989-05-09 | Life Technologies, Inc. | Nucleotide analogs for nucleic acid labeling and detection |
US4739044A (en) * | 1985-06-13 | 1988-04-19 | Amgen | Method for derivitization of polynucleotides |
US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
JP2641880B2 (ja) * | 1986-08-11 | 1997-08-20 | シスカ・ダイアグノスティックス・インコーポレーテッド | 核酸プローブアッセイ法および組成物 |
US5185439A (en) * | 1987-10-05 | 1993-02-09 | Gen-Probe Incorporated | Acridinium ester labelling and purification of nucleotide probes |
EP0972779A3 (de) * | 1987-10-28 | 2004-10-20 | Howard Florey Institute Of Experimental Physiology And Medicine | Oligonucleotid-Polyamid Konjugate |
US5112734A (en) * | 1989-05-26 | 1992-05-12 | Gene-Trak Systems | Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna |
EP0405913B1 (de) * | 1989-06-30 | 1998-12-23 | Chiron Corporation | Hydrophobe Nukleinsäure-Sonde |
US5232829A (en) * | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
WO1991008307A1 (en) * | 1989-12-04 | 1991-06-13 | Microprobe Corporation | Enhanced capture of target nucleic acid by the use of oligonucleotides covalently attached to polymers |
CA2032203C (en) * | 1989-12-29 | 2009-05-19 | Christine L. Brakel | Amplification capture assay |
FR2663040B1 (fr) * | 1990-06-11 | 1995-09-15 | Bio Merieux | Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich. |
US5552541A (en) * | 1990-06-20 | 1996-09-03 | Beckman Instruments, Inc. | Haptenic probes for detecting capture polynucleotides |
US5138045A (en) * | 1990-07-27 | 1992-08-11 | Isis Pharmaceuticals | Polyamine conjugated oligonucleotides |
-
1991
- 1991-07-17 FR FR9109057A patent/FR2679255B1/fr not_active Expired - Fee Related
-
1992
- 1992-07-16 FI FI923260A patent/FI102619B1/fi active IP Right Grant
- 1992-07-17 ES ES92402080T patent/ES2118120T3/es not_active Expired - Lifetime
- 1992-07-17 CA CA002074135A patent/CA2074135C/fr not_active Expired - Fee Related
- 1992-07-17 JP JP4213504A patent/JP2797047B2/ja not_active Expired - Lifetime
- 1992-07-17 DE DE69225646T patent/DE69225646T2/de not_active Expired - Lifetime
- 1992-07-17 EP EP92402080A patent/EP0524864B1/de not_active Expired - Lifetime
- 1992-07-17 AU AU20379/92A patent/AU659176B2/en not_active Ceased
-
1994
- 1994-07-12 US US08/273,776 patent/US5510084A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
FR2679255A1 (fr) | 1993-01-22 |
CA2074135C (fr) | 2001-04-17 |
FI102619B (fi) | 1999-01-15 |
AU2037992A (en) | 1993-01-21 |
FI102619B1 (fi) | 1999-01-15 |
FR2679255B1 (fr) | 1993-10-22 |
DE69225646D1 (de) | 1998-07-02 |
JP2797047B2 (ja) | 1998-09-17 |
JPH05271272A (ja) | 1993-10-19 |
CA2074135A1 (fr) | 1993-01-18 |
EP0524864A1 (de) | 1993-01-27 |
US5510084A (en) | 1996-04-23 |
EP0524864B1 (de) | 1998-05-27 |
FI923260A0 (fi) | 1992-07-16 |
ES2118120T3 (es) | 1998-09-16 |
AU659176B2 (en) | 1995-05-11 |
FI923260A (fi) | 1993-01-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
R071 | Expiry of right |
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