DE69124305T2 - Funkenstrecke zwischen zwei kapillaren elektroden zur fluoreszenzfeststellung bei der kapillarelektrophorese - Google Patents

Funkenstrecke zwischen zwei kapillaren elektroden zur fluoreszenzfeststellung bei der kapillarelektrophorese

Info

Publication number
DE69124305T2
DE69124305T2 DE69124305T DE69124305T DE69124305T2 DE 69124305 T2 DE69124305 T2 DE 69124305T2 DE 69124305 T DE69124305 T DE 69124305T DE 69124305 T DE69124305 T DE 69124305T DE 69124305 T2 DE69124305 T2 DE 69124305T2
Authority
DE
Germany
Prior art keywords
capillary
electrodes
detecting fluorescence
spark
capillary electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69124305T
Other languages
English (en)
Other versions
DE69124305D1 (de
Inventor
Stephen Moring
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of DE69124305D1 publication Critical patent/DE69124305D1/de
Application granted granted Critical
Publication of DE69124305T2 publication Critical patent/DE69124305T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N2030/285—Control of physical parameters of the fluid carrier electrically driven carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
    • G01N2030/8435—Preparation of the fraction to be distributed adding modificating material for chemical reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
DE69124305T 1990-02-28 1991-02-27 Funkenstrecke zwischen zwei kapillaren elektroden zur fluoreszenzfeststellung bei der kapillarelektrophorese Expired - Fee Related DE69124305T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/486,893 US5110431A (en) 1990-02-28 1990-02-28 On-capillary gap junction for fluorescence detection in capillary electrophoresis
PCT/US1991/001274 WO1991013346A1 (en) 1990-02-28 1991-02-27 On-capillary gap junction for fluorescence detection in capillary electrophoresis

Publications (2)

Publication Number Publication Date
DE69124305D1 DE69124305D1 (de) 1997-03-06
DE69124305T2 true DE69124305T2 (de) 1997-06-12

Family

ID=23933585

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69124305T Expired - Fee Related DE69124305T2 (de) 1990-02-28 1991-02-27 Funkenstrecke zwischen zwei kapillaren elektroden zur fluoreszenzfeststellung bei der kapillarelektrophorese

Country Status (4)

Country Link
US (1) US5110431A (de)
EP (1) EP0469146B1 (de)
DE (1) DE69124305T2 (de)
WO (1) WO1991013346A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0356160A3 (de) * 1988-08-24 1991-09-11 The Board Of Trustees Of The Leland Stanford Junior University Kapillarvorrichtung
US5310463A (en) * 1992-11-13 1994-05-10 Board Of Trustees Of The Leland Stanford Junior University On-column junction for capillary columns
US5298134A (en) * 1988-08-24 1994-03-29 Board Of Trustees Of The Leland Stanford Junior University Capillary device
US5269900A (en) * 1990-09-13 1993-12-14 University Of North Carolina At Chapel Hill Method and device for high speed separation of complex molecules
US5180479A (en) * 1991-02-01 1993-01-19 Hewlett-Packard Company Electro-kinetic separation with enlarged input mixing capillary
US5302272A (en) * 1992-03-05 1994-04-12 Beckman Instruments, Inc. Fiber optic flow cell for detection of electrophoresis separation with a capillary column and method of making same
US5384024A (en) * 1992-03-13 1995-01-24 Applied Biosystems, Inc. Capillary electrophoresis
US5637467A (en) * 1992-10-13 1997-06-10 Behringwerke Ag Heterogeneous assay using a pendulous drop
US5605666A (en) * 1993-04-16 1997-02-25 Beckman Instruments, Inc. Capillary retaining system
US5417925A (en) * 1993-04-16 1995-05-23 Beckman Instruments, Inc. Capillary and capillary retaining system
US5449064A (en) * 1994-03-03 1995-09-12 University Of Kansas On-line interface and valve for capillary electophoresis system
US5580523A (en) * 1994-04-01 1996-12-03 Bard; Allen J. Integrated chemical synthesizers
US20050042149A1 (en) * 1994-04-01 2005-02-24 Integrated Chemical Synthesizers, Inc. Nanoscale chemical synthesis
EP0763197A1 (de) * 1994-06-02 1997-03-19 Perkin-Elmer Corporation Verfahren und vorrichtung zur automatischen kohlenhydratkartierung und -sequenzierung
US5798032A (en) * 1994-06-02 1998-08-25 The Perkin-Elmer Corporation Method and apparatus for automated carbohydrate mapping and sequencing
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5661028A (en) * 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
US6110343A (en) * 1996-10-04 2000-08-29 Lockheed Martin Energy Research Corporation Material transport method and apparatus
US6056859A (en) * 1997-02-12 2000-05-02 Lockheed Martin Energy Research Corporation Method and apparatus for staining immobilized nucleic acids
WO1998049344A1 (en) 1997-04-28 1998-11-05 Lockheed Martin Energy Research Corporation Method and apparatus for analyzing nucleic acids
US6062261A (en) * 1998-12-16 2000-05-16 Lockheed Martin Energy Research Corporation MicrofluIdic circuit designs for performing electrokinetic manipulations that reduce the number of voltage sources and fluid reservoirs
AU779947B2 (en) * 1999-03-19 2005-02-17 Governors Of The University Of Alberta, The Automated 2-dimensional analysis of biological and other samples
US6790328B2 (en) 2000-01-12 2004-09-14 Ut-Battelle, Llc Microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
US6709027B2 (en) * 2001-02-23 2004-03-23 Agilent Technologies, Inc. Capillary column sealing technique
JP2006505832A (ja) * 2002-02-26 2006-02-16 フアルマシア・コーポレーシヨン 配列決定システム計算機
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
US9707558B2 (en) * 2009-12-22 2017-07-18 Waters Technologies Corporation Fluidic coupler assembly with conical ferrule
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4676897A (en) * 1985-09-26 1987-06-30 Yokogawa Hokushin Electric Corporation Solubilization chromatography
EP0356160A3 (de) * 1988-08-24 1991-09-11 The Board Of Trustees Of The Leland Stanford Junior University Kapillarvorrichtung
US4936974A (en) * 1988-11-03 1990-06-26 The University Of North Carolina At Chapel Hill Capillary separation system with electric field assisted post separation mixing
US4994165A (en) * 1989-02-16 1991-02-19 Cornell Research Foundation, Inc. Liquid junction coupling for capillary zone electrophoresis/ion spray spectrometry

Also Published As

Publication number Publication date
EP0469146A4 (en) 1993-08-04
DE69124305D1 (de) 1997-03-06
EP0469146B1 (de) 1997-01-22
US5110431A (en) 1992-05-05
WO1991013346A1 (en) 1991-09-05
EP0469146A1 (de) 1992-02-05

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8339 Ceased/non-payment of the annual fee