DE68925893D1 - Sekretorische leader-sequenzen - Google Patents

Sekretorische leader-sequenzen

Info

Publication number
DE68925893D1
DE68925893D1 DE68925893T DE68925893T DE68925893D1 DE 68925893 D1 DE68925893 D1 DE 68925893D1 DE 68925893 T DE68925893 T DE 68925893T DE 68925893 T DE68925893 T DE 68925893T DE 68925893 D1 DE68925893 D1 DE 68925893D1
Authority
DE
Germany
Prior art keywords
leu
ser
phe
ile
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68925893T
Other languages
English (en)
Other versions
DE68925893T2 (de
Inventor
Andrew Goodey
Graham Belfield
Darrell Sleep
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Albumedix Ltd
Original Assignee
Delta Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888817598A external-priority patent/GB8817598D0/en
Priority claimed from GB898906920A external-priority patent/GB8906920D0/en
Application filed by Delta Biotechnology Ltd filed Critical Delta Biotechnology Ltd
Application granted granted Critical
Publication of DE68925893D1 publication Critical patent/DE68925893D1/de
Publication of DE68925893T2 publication Critical patent/DE68925893T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
DE68925893T 1988-07-23 1989-07-14 Sekretorische leader-sequenzen Expired - Lifetime DE68925893T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB888817598A GB8817598D0 (en) 1988-07-23 1988-07-23 Dna & peptide sequences
GB898906920A GB8906920D0 (en) 1989-03-28 1989-03-28 Peptide and dna sequences
PCT/GB1989/000816 WO1990001063A1 (en) 1988-07-23 1989-07-14 New secretory leader sequences

Publications (2)

Publication Number Publication Date
DE68925893D1 true DE68925893D1 (de) 1996-04-11
DE68925893T2 DE68925893T2 (de) 1996-08-08

Family

ID=26294196

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68925893T Expired - Lifetime DE68925893T2 (de) 1988-07-23 1989-07-14 Sekretorische leader-sequenzen

Country Status (15)

Country Link
US (1) US5302697A (de)
EP (1) EP0387319B1 (de)
JP (1) JP3092811B2 (de)
KR (1) KR100195632B1 (de)
AT (1) ATE135045T1 (de)
AU (1) AU633020B2 (de)
CA (1) CA1340547C (de)
DE (1) DE68925893T2 (de)
DK (1) DK175853B1 (de)
FI (1) FI104564B (de)
GB (1) GB2230268B (de)
HU (1) HU213571B (de)
IE (1) IE62266B1 (de)
IL (1) IL91024A (de)
WO (1) WO1990001063A1 (de)

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CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5759802A (en) * 1988-10-26 1998-06-02 Tonen Corporation Production of human serum alubumin A
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
US5766883A (en) * 1989-04-29 1998-06-16 Delta Biotechnology Limited Polypeptides
FR2650598B1 (fr) * 1989-08-03 1994-06-03 Rhone Poulenc Sante Derives de l'albumine a fonction therapeutique
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US5521086A (en) * 1993-09-16 1996-05-28 Cephalon, Inc. Secretion sequence for the production of a heterologous protein in yeast
EP0773296B1 (de) * 1995-02-03 2006-09-13 Asahi Glass Company Ltd. Gen für ein sekretionssignal und dieses enthaltender vektor
US5712114A (en) * 1995-06-06 1998-01-27 Basf Aktiengesellschaft Compositions for expression of proteins in host cells using a preprocollagen signal sequence
GB9526733D0 (en) * 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
GB9712370D0 (en) 1997-06-14 1997-08-13 Aepact Ltd Therapeutic systems
EP1056474B1 (de) * 1997-11-07 2002-06-12 ConjuChem, Inc. Affinitätsmarkierer für menschliches serum albumin
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
EP1274720A4 (de) 2000-04-12 2004-08-18 Human Genome Sciences Inc Albumin enthaltende fusionsproteine
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
US20050244931A1 (en) * 2001-04-12 2005-11-03 Human Genome Sciences, Inc. Albumin fusion proteins
US20060084794A1 (en) * 2001-04-12 2006-04-20 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
AU2002332041A1 (en) * 2001-10-05 2003-04-22 Human Genome Sciences, Inc. Albumin fusion proteins
EP2277888A3 (de) 2001-12-21 2011-04-27 Human Genome Sciences, Inc. Fusionsprotein von Albumin und Erythropoietin
US20080194481A1 (en) 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
EP1463752A4 (de) * 2001-12-21 2005-07-13 Human Genome Sciences Inc Albuminfusionsproteine
JP2005538932A (ja) 2002-02-07 2005-12-22 アベンティス、ベーリング、ゲゼルシャフト、ミット、ベシュレンクテル、ハフツング アルブミン融合クニッツドメインペプチド
GB0217033D0 (en) * 2002-07-23 2002-08-28 Delta Biotechnology Ltd Gene and polypeptide sequences
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
WO2005055944A2 (en) 2003-12-05 2005-06-23 Cincinnati Children's Hospital Medical Center Oligosaccharide compositions and use thereof in the treatment of infection
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
WO2005066348A2 (en) 2004-01-05 2005-07-21 Emd Lexigen Research Center Corp. Interleukin-12 targeted to oncofoetal fibronectin
AU2005317828A1 (en) 2004-12-23 2006-06-29 Novozymes Delta Limited Gene expression technique
EP1816201A1 (de) 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
KR101193722B1 (ko) 2006-07-24 2013-01-11 바이오렉시스 파마슈티칼 코포레이션 엑센딘 융합 단백질
WO2008019368A2 (en) 2006-08-07 2008-02-14 Teva Biopharmaceuticals Usa, Inc. Albumin-insulin fusion proteins
US20090252703A1 (en) * 2006-10-19 2009-10-08 Gegg Jr Colin V Use of alcohol co-solvents to improve pegylation reaction yields
AU2007343796A1 (en) * 2006-10-25 2008-07-24 Amgen Inc. Toxin peptide therapeutic agents
WO2008153745A2 (en) 2007-05-22 2008-12-18 Amgen Inc. Compositions and methods for producing bioactive fusion proteins
CN101835801B (zh) 2007-08-08 2014-09-10 诺维信生物制药丹麦公司 转铁蛋白变体和偶联物
DK2393834T3 (da) 2009-02-06 2013-06-24 Novozymes Biopharma Dk As Fremgangsmåde til oprensning
SG2014012918A (en) 2009-02-11 2014-04-28 Novozymes Biopharma Dk As Albumin variants and conjugates
MX2011009797A (es) 2009-03-20 2012-01-12 Amgen Inc Inhibidores peptidicos de kv1.3 potentes selectivos.
MX2012005864A (es) 2009-11-20 2012-08-31 Amgen Inc Proteinas de enlace al antígeno anti-orai 1 y usos de las mismas.
CN103314296A (zh) 2010-08-10 2013-09-18 安姆根有限公司 用于检测针对靶抗体的中和抗体的双功能体外靶结合测定
EP2725034B1 (de) 2010-09-22 2019-04-03 Amgen Inc. Trägerimmunglobuline die nicht an humane Gewebe binden und Verwendungen davon
US20120185956A1 (en) 2011-01-18 2012-07-19 Gingras Jacinthe Global nav1.7 knockout mice and uses
JP6101638B2 (ja) 2011-03-03 2017-03-22 ザイムワークス，インコーポレイテッド 多価ヘテロマルチマー足場設計及び構築物
AP2013007173A0 (en) 2011-03-16 2013-10-31 Amgen Inc Potent and selective inhibitors of NAV1.3 and NAV1.7
AU2013222188A1 (en) 2012-02-22 2014-09-11 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
AU2013289881B2 (en) 2012-07-13 2018-01-18 Zymeworks Bc Inc. Multivalent heteromultimer scaffold design and constructs
TW201446792A (zh) 2013-03-12 2014-12-16 Amgen Inc Nav1．7之強效及選擇性抑制劑
EP2994164B1 (de) 2013-05-08 2020-08-05 Zymeworks Inc. Bispezifische her2- und her3-antigenbindende konstrukte
EP3039129B1 (de) 2013-08-30 2018-06-27 Amgen Inc. Herstellung rekombinanter aav-vektoren mit hohem titer in haftenden und hängenden zellen
WO2016061141A1 (en) 2014-10-13 2016-04-21 University Of Maryland, Baltimore Methods for treating cardiovascular dysfunction and improving fluid homeostasis with a peptide hormone
US10202616B2 (en) 2014-10-15 2019-02-12 Amgen Inc. Promoter and regulatory elements for improved expression of heterologous genes in host cells
CA2874083C (en) 2014-12-05 2024-01-02 Universite Laval Tdp-43-binding polypeptides useful for the treatment of neurodegenerative diseases
EP3394243A1 (de) 2015-12-22 2018-10-31 Albumedix Ltd. Verbesserte proteinexpressionsstämme
JP6937309B2 (ja) 2016-01-27 2021-09-22 ジャスト−エヴォテック バイオロジックス、インコーポレイテッド ハイブリッドプロモーターおよびその使用
JP7116059B2 (ja) 2016-11-21 2022-08-09 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド アフリベルセプト製剤及びその使用
CN111032871A (zh) 2017-06-20 2020-04-17 阿尔布梅迪克斯医疗有限公司 改良的表达蛋白质的菌株
CN111344410B (zh) 2017-08-17 2023-09-15 济世易为生物有限公司 从宿主细胞半乳糖凝集素和其他污染物中纯化糖基化蛋白的方法
CA3081094A1 (en) 2017-11-20 2019-05-23 Alison J. GILLESPIE Aflibercept formulations containing a lysine salt as tonicifying agent and uses thereof
EP3924458A1 (de) 2019-02-15 2021-12-22 Just-Evotec Biologics, Inc. Automatisierte bioherstellungssysteme, anlagen und prozesse
US11739166B2 (en) 2020-07-02 2023-08-29 Davol Inc. Reactive polysaccharide-based hemostatic agent
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NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
US4588684A (en) * 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
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JP2507874B2 (ja) * 1986-11-27 1996-06-19 工業技術院長 分泌シグナルペプチドをコ−ドするｄｎａ配列

Also Published As

Publication number Publication date
DK175853B1 (da) 2005-04-04
HUT57829A (en) 1991-12-30
GB8928849D0 (en) 1990-07-18
EP0387319B1 (de) 1996-03-06
ATE135045T1 (de) 1996-03-15
DE68925893T2 (de) 1996-08-08
GB2230268A (en) 1990-10-17
KR100195632B1 (ko) 1999-06-15
FI901428A0 (fi) 1990-03-21
JP3092811B2 (ja) 2000-09-25
DK72990D0 (da) 1990-03-21
CA1340547C (en) 1999-05-18
HU213571B (en) 1997-08-28
WO1990001063A1 (en) 1990-02-08
IL91024A (en) 1995-08-31
EP0387319A1 (de) 1990-09-19
DK72990A (da) 1990-03-21
HU894572D0 (en) 1991-05-28
US5302697A (en) 1994-04-12
IE892361L (en) 1990-01-23
AU4042789A (en) 1990-02-19
JPH03500370A (ja) 1991-01-31
IL91024A0 (en) 1990-02-09
FI104564B (fi) 2000-02-29
GB2230268B (en) 1992-01-29
KR920700288A (ko) 1992-02-19
IE62266B1 (en) 1995-01-11
AU633020B2 (en) 1993-01-21

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Owner name: NOVOZYMES DELTA LTD., NOTTINGHAM, GB