DE60229873D1 - Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen - Google Patents

Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen

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Publication number
DE60229873D1
DE60229873D1 DE60229873T DE60229873T DE60229873D1 DE 60229873 D1 DE60229873 D1 DE 60229873D1 DE 60229873 T DE60229873 T DE 60229873T DE 60229873 T DE60229873 T DE 60229873T DE 60229873 D1 DE60229873 D1 DE 60229873D1
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DE
Germany
Prior art keywords
nucleic acid
detecting
uniquely
genedigits
target specific
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Expired - Lifetime
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DE60229873T
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English (en)
Inventor
Krassen Dimitrov
Dwayne Dunaway
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Institute for Systems Biology
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Institute for Systems Biology
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
DE60229873T 2001-07-03 2002-07-03 Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen Expired - Lifetime DE60229873D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/898,743 US7473767B2 (en) 2001-07-03 2001-07-03 Methods for detection and quantification of analytes in complex mixtures
PCT/US2002/021278 WO2003003810A2 (en) 2001-07-03 2002-07-03 Methods for detection and quantification of analytes in complex mixtures

Publications (1)

Publication Number Publication Date
DE60229873D1 true DE60229873D1 (de) 2008-12-24

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DE60229873T Expired - Lifetime DE60229873D1 (de) 2001-07-03 2002-07-03 Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen

Country Status (9)

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US (6) US7473767B2 (de)
EP (1) EP1448581B1 (de)
JP (2) JP4343682B2 (de)
AT (1) ATE414096T1 (de)
AU (1) AU2002327202B2 (de)
CA (3) CA2893908C (de)
DE (1) DE60229873D1 (de)
ES (1) ES2316603T3 (de)
WO (1) WO2003003810A2 (de)

Families Citing this family (210)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
JP4583029B2 (ja) 2001-10-29 2010-11-17 クルセル ホランド ベー ヴェー 所定の翻訳後修飾を有する蛋白質の製造方法及び製造手段
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
AU2003228649A1 (en) * 2002-04-23 2003-11-10 U.S.Genomics, Inc. Compositions and methods related to two-arm nucleic acid probes
AU2003251986A1 (en) * 2002-07-17 2004-02-02 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
US7632548B2 (en) * 2002-08-02 2009-12-15 Applied Nanotech Holdings, Inc. Remote identification of explosives and other harmful materials
WO2005059172A2 (en) * 2003-12-11 2005-06-30 Epigenomics Ag Method and nucleic acids for the improved treatment of breast cell proliferative disorders
JP4865539B2 (ja) 2003-05-09 2012-02-01 クルセル ホランド ベー ヴェー E1不死化細胞の培養物及び該培養物を培養して該培養物から得られる産物の収量を増加させる方法
US20040265833A1 (en) * 2003-06-23 2004-12-30 Cathy Lofton-Day Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
WO2005001141A2 (en) 2003-06-23 2005-01-06 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
ES2546644T3 (es) 2003-06-23 2015-09-25 Epigenomics Ag Métodos y ácidos nucleicos para el análisis de trastornos proliferativos de células colorrectales
US7674628B2 (en) * 2003-08-01 2010-03-09 Applied Nanotech Holdings, Inc. Remote identification of explosives and other harmful materials
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
WO2005050171A2 (en) * 2003-11-17 2005-06-02 U.S. Genomics, Inc. Methods and compositions relating to single reactive center reagents
JP4824575B2 (ja) * 2003-12-01 2011-11-30 エピゲノミクス アクチェンゲゼルシャフト 前立腺細胞増殖性疾患の発症に関連する遺伝子発現の分析のための方法および核酸
EP1561821B1 (de) 2003-12-11 2011-02-16 Epigenomics AG Marker zur Prognose der Reaktion auf Therapie bzw. der Überlebensrate von Brustkrebspatienten
WO2005067692A2 (en) * 2004-01-13 2005-07-28 U.S. Genomics, Inc. Detection and quantification of analytes in solution using polymers
US20050169415A1 (en) * 2004-01-30 2005-08-04 Agere Systems Inc. Timing error recovery system
WO2006008128A2 (en) 2004-07-18 2006-01-26 Epigenomics Ag Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
US8029985B2 (en) * 2004-09-01 2011-10-04 Vybion, Inc. Amplified bioassay
EP1812589A2 (de) * 2004-09-30 2007-08-01 Epigenomics AG Epigenetische verfahren und nukleinsäuren zum nachweis von lungenzellproliferationsstörungen
EP2280084B1 (de) 2004-12-02 2015-02-11 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von mit der Prognose von Störungen der Proliferation von Prostatazellen assoziierter Genexpression
US20070009884A1 (en) * 2005-04-11 2007-01-11 Ghc Technologies, Inc. Methods and apparatuses for detecting chemical or biological agents
KR101708544B1 (ko) 2005-04-15 2017-02-20 에피제노믹스 아게 세포 증식 질환을 분석하기 위한 방법 및 핵산
ES2446250T3 (es) 2005-05-02 2014-03-06 University Of Southern California Marcadores de metilación del ADN asociados con el fenotipo metilador de islas CpG (CIMP) en el cáncer colorrectal humano
US10053735B2 (en) * 2005-09-21 2018-08-21 Therawis Diagnostics Gmbh Markers for the prediction of outcome of anthracycline treatment
WO2007039234A2 (en) 2005-09-29 2007-04-12 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with tissue classification
EP1943356B1 (de) * 2005-10-03 2014-05-14 Epigenomics AG Verfahren und nukleinsäuren zur analyse der genexpression in zusammenhang mit der prognose von zellproliferationsstörungen
US7770717B2 (en) * 2005-10-12 2010-08-10 Scanvaegt International A/S Device for transfer of items
AU2006330834B2 (en) * 2005-12-23 2013-09-12 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
AU2013203290B2 (en) * 2005-12-23 2016-05-05 Institute For Systems Biology Nanoreporters and methods of manufacturing and use thereof
US20100015607A1 (en) * 2005-12-23 2010-01-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
JP4621595B2 (ja) * 2006-01-11 2011-01-26 株式会社東芝 半導体装置の製造方法
US20090317810A1 (en) * 2006-04-17 2009-12-24 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
CA2649777C (en) 2006-04-17 2018-08-14 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US7964347B2 (en) * 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
US7312029B1 (en) 2006-06-30 2007-12-25 Searete Llc Method of combing an elongated molecule
EP2044221B1 (de) * 2006-07-21 2015-07-22 Epigenomics AG Verfahren und kit zur analyse der methylierung in kolorektalen proliferativen zellerkrankungen
WO2008009478A1 (en) * 2006-07-21 2008-01-24 Epigenomics Ag Methods and nucleic acids for analyses of cellular proliferative disorders
AU2007281479A1 (en) * 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
JP5244103B2 (ja) 2006-08-09 2013-07-24 ホームステッド クリニカル コーポレイション 器官特異的蛋白質およびその使用方法
US20080050724A1 (en) * 2006-08-24 2008-02-28 Microfluidic Systems, Inc. Method of detecting one or more limited copy targets
US7970534B2 (en) 2006-08-24 2011-06-28 Blackbird Technologies, Inc. Mobile unit and system having integrated mapping, communications and tracking
JP5562034B2 (ja) 2006-11-24 2014-07-30 エピゲノミクス アーゲー 前立腺細胞増殖性障害の発症に関連する遺伝子の発現を解析する方法及び核酸
WO2008088839A2 (en) * 2007-01-16 2008-07-24 Cytocure, Inc. Methods of isolating and purifying nucleic acid-binding biomolecules and compositions including same
DK2258871T3 (da) 2007-01-19 2014-08-11 Epigenomics Ag Fremgangsmåder og nukleinsyrer til analyse af celleproliferative lidelser
US20090203011A1 (en) * 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
US7843335B2 (en) 2007-03-13 2010-11-30 Blackbird Technologies, Inc. Mobile asset tracking unit, system and method
JP5555157B2 (ja) * 2007-04-10 2014-07-23 ナノストリング テクノロジーズ, インコーポレイテッド ナノレポーターにおける使用のための標的特異的配列を同定するための方法およびコンピュータシステム
WO2009012340A2 (en) 2007-07-16 2009-01-22 California Institute Of Technology Microfluidic devices, methods and systems for detecting target molecules
AU2008334901A1 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of lung carcinoma
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
EP2370820B1 (de) 2008-12-31 2016-02-24 Abbott Point Of Care, Inc. Verfahren und vorrichtung für einen immuntest unter verwendung von nukleotidkonjugaten
EP2966181B1 (de) 2009-06-26 2018-02-14 Epigenomics AG Verfahren und nukleinsäuren zur analyse des blasenkarzinom
ES2534200T3 (es) * 2009-08-03 2015-04-20 Epigenomics Ag Métodos para la preservación de la complejidad de la secuencia del ADN genómico
CA2775613C (en) * 2009-10-13 2018-06-12 Nanostring Technologies, Inc. Protein detection via nanoreporters
AU2015261558B2 (en) * 2009-10-13 2017-09-28 Nanostring Technologies, Inc Protein Detection Via Nanoreporters
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
JP2013509883A (ja) 2009-11-06 2013-03-21 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 臓器移植患者における移植片拒絶反応の非侵襲的診断方法
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2012012141A1 (en) 2010-06-30 2012-01-26 Amgen Inc. Scnn1a/tnfrsf1a fusion proteins in cancer
EP2619321B1 (de) * 2010-09-20 2018-08-01 Advanced Cell Diagnostics, Inc. Biomarker zur differenzierung eines melanoms von einem gutartigen muttermal auf der haut
WO2016100976A2 (en) 2014-12-19 2016-06-23 Apprise Bio, Inc. Methods for identifying multiple epitopes in selected sub-populations of cells
JP6069224B2 (ja) 2011-01-31 2017-02-01 アプライズ バイオ, インコーポレイテッド 細胞において複数のエピトープを同定する方法
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8538373B2 (en) 2011-05-25 2013-09-17 Blackbird Technologies, Inc. Methods and apparatus for emergency tracking
AU2012282528B2 (en) 2011-07-08 2017-04-13 Epigenomics Ag Methods and nucleic acids for determining the prognosis of a cancer subject
AU2012312353A1 (en) 2011-09-22 2014-04-10 Lineage Biosciences, Inc. Compositions and methods for analyzing heterogeneous samples
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
ES2953308T3 (es) 2011-12-22 2023-11-10 Harvard College Composiciones y métodos para la detección de analitos
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
EP4361609A2 (de) 2012-02-03 2024-05-01 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US9758829B2 (en) 2012-06-22 2017-09-12 Htg Molecular Diagnostics, Inc. Molecular malignancy in melanocytic lesions
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
EP2888349B1 (de) 2012-08-24 2022-02-09 Yale University System, vorrichtung und verfahren für multiplex-erkennung mit hohem durchsatz
WO2014071247A1 (en) 2012-11-02 2014-05-08 Dana-Farber Cancer Institute, Inc. Pyrrol-1 -yl benzoic acid derivates useful as myc inhibitors
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
EP3415626B1 (de) 2013-03-15 2020-01-22 Lineage Biosciences, Inc. Verfahren und zusammensetzungen zum markieren und analysieren von proben
US20140287949A1 (en) * 2013-03-15 2014-09-25 Institute For Systems Biology Multiplex assay for members of binding pairs
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
CA2919833A1 (en) 2013-07-30 2015-02-05 President And Fellows Of Harvard College Quantitative dna-based imaging and super-resolution imaging
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
CA2929826C (en) 2013-11-06 2022-08-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for selecting and treating lymphoma types
US10011811B2 (en) 2013-11-25 2018-07-03 Vericel Corporation Cell seeding device and method
US10240208B2 (en) 2014-03-31 2019-03-26 H. Lee Moffitt Cancer Center And Research Institute, Inc. MicroRNA assay for detection and management of pancreatic cancer precursors
JP2017516501A (ja) 2014-05-30 2017-06-22 ジーンセントリック ダイアグノスティクス, インコーポレイテッド 肺がんのタイピング法
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP3175023A4 (de) 2014-07-30 2018-03-07 President and Fellows of Harvard College Systeme und verfahren zur bestimmung von nukleinsäuren
JP2017530693A (ja) 2014-08-08 2017-10-19 ナノストリング テクノロジーズ,インコーポレイティド 遺伝子発現データを使用した混成細胞集団のデコンボリューション方法
JP2017529103A (ja) * 2014-09-22 2017-10-05 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニアThe Regents of the University of California 単分子rna検出
CN107208144B (zh) 2014-11-21 2021-06-08 纳米线科技公司 无酶且无扩增的测序
JP7144142B2 (ja) 2014-11-24 2022-09-29 ナノストリング テクノロジーズ,インコーポレイティド 遺伝子精製および画像化のための方法および装置
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
EP3835431B1 (de) 2015-03-30 2022-11-02 Becton, Dickinson and Company Verfahren für kombinatorische barcodierung
EP3530752B1 (de) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
EP3286326A1 (de) 2015-04-23 2018-02-28 Cellular Research, Inc. Verfahren und zusammensetzungen zur gesamttranskriptomamplifikation
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
WO2017015099A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
CA2992480A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
WO2017040526A2 (en) 2015-09-01 2017-03-09 Eisai R&D Management Co., Ltd. Splice variants associated with neomorphic sf3b1 mutants
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド ライブラリー正規化のための方法および組成物
CA3000405A1 (en) 2015-09-29 2017-04-06 Htg Molecular Diagnostics, Inc. Methods for subtyping diffuse large b-cell lymphoma (dlbcl)
ES2893100T3 (es) 2015-10-12 2022-02-08 Advanced Cell Diagnostics Inc Detección in situ de variantes de nucleótidos en muestras con un elevado nivel de ruido, y composiciones y métodos relacionados con ésta
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
CN108884495B (zh) 2016-02-08 2023-08-22 美国政府(由卫生和人类服务部的部长所代表) 基因标记预测肝细胞癌对经导管动脉化疗栓塞(tace)的应答
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
CA3021569A1 (en) 2016-04-20 2017-10-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Evaluation of mantle cell lymphoma and methods related thereto
CN109415761B (zh) 2016-04-25 2022-09-20 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
EP4029950A1 (de) 2016-04-29 2022-07-20 Board of Regents, The University of Texas System Gezielte messung der transkriptionellen aktivität im zusammenhang mit hormonrezeptoren
EP4324929A1 (de) 2016-05-16 2024-02-21 Nanostring Technologies, Inc. Verfahren zur erkennung von zielnukleinsäuren in einer probe
CN109863251B (zh) 2016-05-17 2022-11-18 基因中心治疗公司 对肺鳞状细胞癌亚型分型的方法
CA3024747A1 (en) 2016-05-17 2017-11-23 Genecentric Therapeutics, Inc. Methods for subtyping of lung adenocarcinoma
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3472354A4 (de) 2016-06-17 2020-01-01 California Institute of Technology Nukleinsäurereaktionen sowie zugehörige verfahren und zusammensetzungen
EP3472359B1 (de) 2016-06-21 2022-03-16 10X Genomics, Inc. Nukleinsäuresequenzierung
CN109923216A (zh) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN109983125A (zh) 2016-08-31 2019-07-05 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
DK3538891T3 (da) 2016-11-11 2022-03-28 Isoplexis Corp Sammensætninger og fremgangsmåder til samtidig genomisk, transkriptomisk og proteomisk analyse af enkeltceller
CN116334202A (zh) 2016-11-21 2023-06-27 纳米线科技公司 化学组合物及其使用方法
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法,以及其制造方法
RU2637623C1 (ru) * 2016-12-27 2017-12-05 Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский национальный исследовательский медицинский университет им. Н.И. Пирогова" Министерства здравоохранения Российской Федерации (ФГБОУ ВО РНИМУ им. Н.И. Пирогова Минздрава России) Способ оценки риска развития онкозаболеваний в поколениях жителей регионов радионуклидного загрязнения
EP3577232A1 (de) 2017-02-01 2019-12-11 Cellular Research, Inc. Selektive amplifikation unter verwendung von blockierenden oligonukleotiden
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
EP3638814B8 (de) 2017-06-14 2023-06-21 The United States of America, as Represented by The Secretary, Department of Health and Human Services Verfahren zur bestimmung des lymphomtyps
US11739386B2 (en) 2017-07-21 2023-08-29 Genecentric Therapeutics, Inc. Methods for determining response to PARP inhibitors
CN111263819A (zh) 2017-10-06 2020-06-09 卡特阿纳公司 Rna模板化连接
EP3697924A1 (de) 2017-10-16 2020-08-26 Enterome Neue instrumente zur beurteilung der therapeutischen wirksamkeit von fimh-blockern
EP3728636A1 (de) 2017-12-19 2020-10-28 Becton, Dickinson and Company Mit oligonukleotiden assoziierte teilchen
JP7372927B6 (ja) 2018-02-12 2023-11-27 ナノストリング テクノロジーズ,インコーポレイティド 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
WO2019158705A1 (en) 2018-02-16 2019-08-22 The Institute Of Cancer Research: Royal Cancer Hospital Patient classification and prognostic method
EP3788170A1 (de) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
EP3788171B1 (de) 2018-05-03 2023-04-05 Becton, Dickinson and Company Multiomics-probenanalyse mit hohem durchsatz
WO2019213478A1 (en) 2018-05-04 2019-11-07 Nanostring Technologies, Inc. Gene expression assay for measurement of dna mismatch repair deficiency
KR20210061962A (ko) 2018-05-14 2021-05-28 나노스트링 테크놀로지스, 인크. 화학 조성물 및 이의 사용 방법
US20210363590A1 (en) 2018-05-21 2021-11-25 Nanostring Technologies, Inc. Molecular gene signatures and methods of using same
GB201810190D0 (en) 2018-06-21 2018-08-08 Cancer Research Tech Ltd Prognostic and treatment response predictive method
CN112770776A (zh) 2018-07-30 2021-05-07 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
EP3861134A1 (de) 2018-10-01 2021-08-11 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
CA3115922A1 (en) 2018-10-09 2020-04-16 Genecentric Therapeutics, Inc. Detecting cancer cell of origin
EP3874057A4 (de) * 2018-11-01 2022-08-17 Advanced Cell Diagnostics, Inc. Verfahren zum digitalen in-situ-multiplexen von nukleinsäuren
JP2022506546A (ja) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
EP3894587A1 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Auflösung von räumlichen anordnungen durch nähebasierte dekonvolution
EP3894552A1 (de) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
AU2020282024A1 (en) 2019-05-31 2021-11-11 10X Genomics, Inc. Method of detecting target nucleic acid molecules
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のdna配列を同定する方法
US20230059203A1 (en) * 2019-12-19 2023-02-23 Akoya Biosciences, Inc. RNA Detection
EP3891300B1 (de) 2019-12-23 2023-03-29 10X Genomics, Inc. Verfahren zur räumlichen analyse mittels rna-template-ligation
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4162074B1 (de) 2020-06-08 2024-04-24 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4168578A1 (de) 2020-06-18 2023-04-26 Nanostring Technologies, Inc. Zusammensetzungen und verfahren zur in-situ-einzelzellenanalyse unter verwendung von enzymatischer nukleinsäureverlängerung
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
GB2597332A (en) 2020-07-20 2022-01-26 Institute Of Cancer Res Prognostic and treatment response predictive method
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
GB202015200D0 (en) 2020-09-25 2020-11-11 Institute Of Cancer Res Royal Cancer Hospital The Prognostic and treatment response predictive method
CA3199753A1 (en) 2020-11-04 2022-05-12 Eisai R&D Management Co., Ltd. Biomarkers for myelodysplastic syndrome (mds) and methods of using the same
US20220145389A1 (en) * 2020-11-06 2022-05-12 Waters Technologies Corporation Methods and compositions useful for nucleic acid analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (de) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profilierung von stark exprimierten und schwach exprimierten proteinen
EP4012046A1 (de) 2020-12-11 2022-06-15 10X Genomics, Inc. Verfahren und zusammensetzungen zur multimodalen in-situ-analyse
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220235403A1 (en) 2021-01-26 2022-07-28 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022266416A1 (en) 2021-06-17 2022-12-22 Nanostring Technologies, Inc. Compositions and methods for in situ single cell analysis using enzymatic nucleic acid extension
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US20230057571A1 (en) 2021-08-03 2023-02-23 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
EP4137585A1 (de) 2021-08-20 2023-02-22 Institut D'Investigacions Biomèdiques August Pi I Sunyer - IDIBAPS Krebsinformationsbiomarkersignatur
EP4196605A1 (de) 2021-09-01 2023-06-21 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
GB202116745D0 (en) 2021-11-19 2022-01-05 Institute Of Cancer Res Royal Cancer Hospital Prognostic and treatment response predictive method
WO2023131866A1 (en) 2022-01-05 2023-07-13 Eisai R&D Management Co., Ltd. Biomarkers for myelodysplastic syndrome (mds) and methods of using the same
US20230279475A1 (en) 2022-01-21 2023-09-07 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
WO2024079279A1 (en) 2022-10-12 2024-04-18 F. Hoffmann-La Roche Ag Disease characterisation

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2825529C2 (de) * 1978-06-10 1980-11-13 Manfred Kammer Stückförmiges Reinigungsmittel, insbesondere Seife
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
US4824775A (en) * 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
US5175270A (en) 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
US4824447A (en) * 1986-12-30 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Enhanced oil recovery system
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5763162A (en) * 1990-03-14 1998-06-09 The Regents Of University Of California Multichromophore fluorescent DNA intercalation complexes
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
WO1994016104A1 (en) * 1993-01-08 1994-07-21 Ctrc Research Foundation Color imaging system for use in molecular biology
US5293050A (en) 1993-03-25 1994-03-08 International Business Machines Corporation Semiconductor quantum dot light emitting/detecting devices
US5543838A (en) 1993-08-31 1996-08-06 Xerox Corporation Signal multiplexing system for an image sensor array
US5679519A (en) * 1995-05-09 1997-10-21 Oprandy; John J. Multi-label complex for enhanced sensitivity in electrochemiluminescence assay
AU6898296A (en) 1995-08-14 1997-03-12 Ely Michael Rabani Methods and devices for parallel multiplex polynucleotide sequencing
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6277583B1 (en) * 1996-02-07 2001-08-21 Conjuchem, Inc. Affinity labeling libraries and applications thereof
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20020150921A1 (en) 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7144119B2 (en) 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
EP0912761A4 (de) 1996-05-29 2004-06-09 Cornell Res Foundation Inc Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
ES2287956T3 (es) * 1996-07-29 2007-12-16 Nanosphere Inc. Nanoparticulas que tienen oligonucleotidos unidos a las mismas y usos de las mismas.
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
AU748939B2 (en) 1997-02-20 2002-06-13 Regents Of The University Of California, The Plasmon resonant particles, methods and apparatus
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
AU9220498A (en) * 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
HUP0003986A3 (en) 1997-10-14 2001-04-28 Luminex Corp Austin Precision fluorescently dyed particles and methods of making and using same
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
ATE239801T1 (de) * 1998-01-22 2003-05-15 Luminex Corp Mikropartikel mit multiplen fluoreszenz-signalen
ATE333499T1 (de) 1998-03-18 2006-08-15 Quark Biotech Inc Selektion/subtraktionsansatz zur genidentifizierung
AU3555599A (en) * 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
AU758466B2 (en) 1998-06-02 2003-03-20 Yale University Multiparametric fluorescence in situ hybridization
AU754952B2 (en) 1998-06-24 2002-11-28 Illumina, Inc. Decoding of array sensors with microspheres
ATE234468T1 (de) 1998-09-18 2003-03-15 Massachusetts Inst Technology Biologische verwendungen von halbleitenden nanokristallen
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
EP1006199A1 (de) 1998-12-03 2000-06-07 Kreatech Biotechnology B.V. Verfahren zur Herstellung von Verknüpfungen an ausgewählten Positionen von Nukleinsäuresträngen und deren Verwendung
JP4014742B2 (ja) 1998-12-04 2007-11-28 キヤノンファインテック株式会社 シート処理装置及び画像形成装置
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
EP1141384A2 (de) * 1999-01-06 2001-10-10 Cornell Research Foundation, Inc. Verfahren zur beschleunigung der identifizierung von mononukleotid-polymorphismen und der ausrichtung von klonen bei genomischer sequenzierung
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO2000056937A2 (en) * 1999-03-25 2000-09-28 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
EP1171636A2 (de) * 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Bestimmungsmethode für gen expressionsmustern durch multi fluor fish
AU4701200A (en) 1999-05-07 2000-11-21 Quantum Dot Corporation A method of detecting an analyte using semiconductor nanocrystals
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001000875A2 (en) 1999-06-25 2001-01-04 Motorola, Inc. Novel methods and products for arrayed microsphere analysis
JP2003503591A (ja) * 1999-06-29 2003-01-28 ダコ エー エス 標識およびプローブを保持するデンドリマーを用いた検出
AU2246601A (en) * 1999-08-30 2001-04-10 Illumina, Inc. Methods for improving signal detection from an array
JP2004501340A (ja) * 2000-01-13 2004-01-15 ナノスフェアー インコーポレイテッド オリゴヌクレオチドを付着させたナノ粒子とその使用方法
WO2003074738A1 (en) * 2000-01-18 2003-09-12 Quantom Dot Corporation Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization
US20020028457A1 (en) * 2000-02-16 2002-03-07 Quantum Dot Corporation Single target counting assays using semiconductor nanocrystals
WO2001062378A2 (en) 2000-02-22 2001-08-30 Genospectra, Inc. Microarray fabrication techniques and apparatus
US20030186426A1 (en) 2000-03-15 2003-10-02 The Regents Of The University Of California Multichannel flow cell for interacting single optically trapped, DNA molecules with different chemical species
US6759235B2 (en) 2000-04-06 2004-07-06 Quantum Dot Corporation Two-dimensional spectral imaging system
US20020034827A1 (en) 2000-08-01 2002-03-21 Rajendra Singh Methods for solid phase nanoextraction and desorption
AU2002211807A1 (en) 2000-09-11 2002-03-26 Massachusetts Institute Of Technology Direct haplotyping using carbon nanotube probes
US6777244B2 (en) 2000-12-06 2004-08-17 Hrl Laboratories, Llc Compact sensor using microcavity structures
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
JP4146239B2 (ja) 2001-03-28 2008-09-10 ナノスフェアー インコーポレイテッド オリゴヌクレオチド修飾粒子をベースとするバイオバーコード
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures

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