DE602005025104D1 - Mutierte glucosedehydrogenase - Google Patents

Mutierte glucosedehydrogenase

Info

Publication number
DE602005025104D1
DE602005025104D1 DE602005025104T DE602005025104T DE602005025104D1 DE 602005025104 D1 DE602005025104 D1 DE 602005025104D1 DE 602005025104 T DE602005025104 T DE 602005025104T DE 602005025104 T DE602005025104 T DE 602005025104T DE 602005025104 D1 DE602005025104 D1 DE 602005025104D1
Authority
DE
Germany
Prior art keywords
glucose dehydrogenase
mutated glucose
amino acid
acid residue
mutated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005025104T
Other languages
English (en)
Inventor
Hideaki Yamaoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arkray Inc
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Publication of DE602005025104D1 publication Critical patent/DE602005025104D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
DE602005025104T 2004-04-23 2005-04-22 Mutierte glucosedehydrogenase Active DE602005025104D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004128165 2004-04-23
PCT/JP2005/007687 WO2005103248A1 (ja) 2004-04-23 2005-04-22 変異グルコース脱水素酵素

Publications (1)

Publication Number Publication Date
DE602005025104D1 true DE602005025104D1 (de) 2011-01-13

Family

ID=35196974

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005025104T Active DE602005025104D1 (de) 2004-04-23 2005-04-22 Mutierte glucosedehydrogenase

Country Status (8)

Country Link
US (2) US7803592B2 (de)
EP (1) EP1739174B1 (de)
JP (1) JP4639302B2 (de)
KR (1) KR101186718B1 (de)
CN (1) CN1973036B (de)
AT (1) ATE490316T1 (de)
DE (1) DE602005025104D1 (de)
WO (1) WO2005103248A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7514250B2 (en) 2002-12-24 2009-04-07 Ikeda Food Research Co., Ltd. Coenzyme-binding glucose dehydrogenase
CN101558992B (zh) * 2003-09-02 2012-01-04 早出广司 葡萄糖传感器和葡萄糖浓度测定装置
JP5020070B2 (ja) 2005-03-25 2012-09-05 池田食研株式会社 補酵素結合型グルコース脱水素酵素をコードするポリヌクレオチド
EP2077321B1 (de) 2005-06-20 2011-08-17 ARKRAY, Inc. Mutierte Glucose-Dehydrogenase
WO2008001903A1 (en) 2006-06-29 2008-01-03 Ikeda Food Research Co., Ltd. Fad-conjugated glucose dehydrogenase gene
ATE496124T1 (de) * 2006-11-14 2011-02-15 Toyo Boseki Modifizierte flavin-adenin-dinucleotid-abhängige glucose-dehydrogenase
WO2009084616A1 (ja) * 2007-12-28 2009-07-09 Ikeda Food Research Co., Ltd. 改変型グルコース脱水素酵素遺伝子
JP5803081B2 (ja) * 2009-10-09 2015-11-04 東洋紡株式会社 Ｆａｄジヌクレオチド依存性グルコースデヒドロゲナーゼの温度依存性を改善する方法
JP5824205B2 (ja) * 2010-10-27 2015-11-25 アークレイ株式会社 変異グルコース脱水素酵素
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
EP2636733A1 (de) 2012-03-08 2013-09-11 Universität für Bodenkultur Wien Mutierte Cellobiose-Dehydrogenase mit erhöhter Substratspezifität
WO2016087937A2 (en) * 2014-12-04 2016-06-09 Smartzyme Innovation Ltd. Compositions and methods for measuring blood glucose levels
CN107532152A (zh) * 2014-12-04 2018-01-02 智慧酶生物医药有限公司 用于测量血糖水平的组合物和方法
CN106349392A (zh) * 2015-07-14 2017-01-25 达尔生技股份有限公司 一种融合多肽
AU2016295361A1 (en) 2015-07-23 2018-02-22 Smartzyme Biopharma Ltd. Compositions and methods for measuring blood glucose levels
EP3249050B1 (de) 2016-05-23 2019-01-23 ARKRAY, Inc. Enzymelektrode und biosensor mit verwendung davon
CN108251391A (zh) * 2017-08-18 2018-07-06 青岛蔚蓝生物集团有限公司 新型葡萄糖氧化酶突变体
CN109425643B (zh) 2017-08-25 2022-10-25 爱科来株式会社 基于酶电化学阻抗测量法的新型生物传感技术
CN108486027A (zh) * 2018-04-04 2018-09-04 江南大学 一种fad为辅基的葡萄糖脱氢酶的生产、纯化方法
US11608515B2 (en) 2018-05-22 2023-03-21 Arkray, Inc. Biosensing method
EP3640344B1 (de) 2018-10-03 2024-03-13 ARKRAY, Inc. Direkt-elektronen-transfer-oxidoreduktase-modifiziertes molekulares erkennungselement
CN111593030B (zh) * 2020-04-17 2022-11-08 华东理工大学 化学耐受性的葡萄糖脱氢酶突变体及其在辅酶再生中的应用
EP4067483A1 (de) 2021-03-29 2022-10-05 The University of North Carolina at Chapel Hill Mutante glucose-dehydrogenase mit verbesserter thermischer stabilität und deren verwendung

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10243786A (ja) * 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
JP2000312588A (ja) * 1999-04-30 2000-11-14 Koji Hayade グルコース脱水素酵素
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
PL207576B1 (pl) 2000-10-31 2011-01-31 Koji Sode Białko, DNA, rekombinowany wektor, transformant, sposób wytwarzania białka o aktywności dehydrogenazy glukozowej, szczep Burkhorderia cepacia KS1 (FERM BP-7306) oraz sensor glukozy i zestaw do oznaczania glukozy
JP4036667B2 (ja) * 2002-03-25 2008-01-23 広司 早出 新規グルコース脱水素酵素及びそれをコードする遺伝子
DE60316424T2 (de) 2002-04-26 2008-06-26 Koji Sode Beta-untereinheit von glucosedehydrogenase und diese kodierende dna
US7476525B2 (en) 2002-05-27 2009-01-13 Toyo Boseki Kabushiki Kaisha Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability
AU2003262258A1 (en) * 2002-08-30 2004-03-29 Arkray, Inc. Method of purifying protein and glucose dehydrogenase
JP4102138B2 (ja) * 2002-08-30 2008-06-18 広司 早出 グルコース脱水素酵素の製造方法

Also Published As

Publication number Publication date
JP4639302B2 (ja) 2011-02-23
CN1973036A (zh) 2007-05-30
JPWO2005103248A1 (ja) 2008-03-13
US20080280312A1 (en) 2008-11-13
KR101186718B1 (ko) 2012-09-27
US8012729B2 (en) 2011-09-06
WO2005103248A1 (ja) 2005-11-03
EP1739174A1 (de) 2007-01-03
US20110076707A1 (en) 2011-03-31
EP1739174B1 (de) 2010-12-01
EP1739174A4 (de) 2008-01-09
US7803592B2 (en) 2010-09-28
CN1973036B (zh) 2014-07-23
KR20070006921A (ko) 2007-01-11
ATE490316T1 (de) 2010-12-15

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