DE602004008241D1 - Verfahren zum anfärben von sperma - Google Patents
Verfahren zum anfärben von spermaInfo
- Publication number
- DE602004008241D1 DE602004008241D1 DE602004008241T DE602004008241T DE602004008241D1 DE 602004008241 D1 DE602004008241 D1 DE 602004008241D1 DE 602004008241 T DE602004008241 T DE 602004008241T DE 602004008241 T DE602004008241 T DE 602004008241T DE 602004008241 D1 DE602004008241 D1 DE 602004008241D1
- Authority
- DE
- Germany
- Prior art keywords
- sperm
- coloring
- cells
- sperm cells
- involve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000004040 coloring Methods 0.000 title 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000000684 flow cytometry Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US45873103P | 2003-03-28 | 2003-03-28 | |
US458731P | 2003-03-28 | ||
PCT/US2004/009903 WO2004087177A1 (en) | 2003-03-28 | 2004-03-29 | Process for the staining of sperm |
Publications (2)
Publication Number | Publication Date |
---|---|
DE602004008241D1 true DE602004008241D1 (de) | 2007-09-27 |
DE602004008241T2 DE602004008241T2 (de) | 2008-05-08 |
Family
ID=33131822
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602004008241T Expired - Lifetime DE602004008241T2 (de) | 2003-03-28 | 2004-03-29 | Verfahren zum anfärben von sperma |
Country Status (14)
Country | Link |
---|---|
US (1) | US7335507B2 (de) |
EP (1) | EP1608382B1 (de) |
JP (1) | JP2006525019A (de) |
CN (1) | CN100379429C (de) |
AT (1) | ATE369867T1 (de) |
AU (1) | AU2004226343B2 (de) |
BR (1) | BRPI0408852A (de) |
CA (1) | CA2519989A1 (de) |
DE (1) | DE602004008241T2 (de) |
MX (1) | MXPA05010478A (de) |
NZ (1) | NZ542688A (de) |
PL (1) | PL1608382T3 (de) |
WO (1) | WO2004087177A1 (de) |
ZA (1) | ZA200507823B (de) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2264428B1 (de) * | 1997-01-31 | 2017-05-03 | Xy, Llc | Optisches Gerät mit fokussierendem Reflektor zur Konvergenz von Strahlung auf einen Partikelfluss |
US6149867A (en) * | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US6071689A (en) * | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
NZ527659A (en) | 1998-07-30 | 2006-02-24 | Colorado State University Thro | Equine artificial insemination when there has been sex selection of the sperm to produce an equine of the desired sex |
US7208265B1 (en) * | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
AR034121A1 (es) | 2000-05-09 | 2004-02-04 | Xy Inc | Metodo para aislar celulas de esperma que contienen cromosoma x de celulas de esperma que contienen cromosoma y |
JP2004503231A (ja) * | 2000-06-12 | 2004-02-05 | エックスワイ,インコーポレイテッド | X染色体保有精子およびy染色体保有精子の単離された集団を利用する統合群れ管理システム |
US20040031071A1 (en) * | 2000-10-05 | 2004-02-12 | Xy, Inc. | System of hysteroscopic insemination of mares |
AU2002220018A1 (en) | 2000-11-29 | 2002-06-11 | Colorado State University | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
AU2003265362B2 (en) | 2002-08-01 | 2009-11-05 | Xy, Llc. | Low pressure sperm cell separation system |
BRPI0313476B1 (pt) * | 2002-08-15 | 2015-06-23 | Xy Llc | Citômetro de fluxo de alta resolução |
US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
DE602004024874D1 (de) | 2003-03-28 | 2010-02-11 | Inguran Llc | Eschlechts-sortierten tierspermien |
WO2004087177A1 (en) | 2003-03-28 | 2004-10-14 | Monsanto Technology Llc | Process for the staining of sperm |
AU2004242121B2 (en) * | 2003-05-15 | 2010-06-24 | Xy, Llc. | Efficient haploid cell sorting for flow cytometer systems |
EP1730523B1 (de) | 2004-03-29 | 2010-01-13 | Inguran, LLC | Verwendung einer oxidations-/reduktionsreaktionen intrazellulär und/oder extrazellulär regulierenden zusammensetzung in einem anfärbe- oder sortierungsverfahren für spermatozoen |
ES2397678T3 (es) * | 2004-03-29 | 2013-03-08 | Inguran, Llc | Suspensiones de espermatozoides para clasificación en poblaciones enriquecidas portadoras del cromosoma X o Y |
MX2007000888A (es) | 2004-07-22 | 2007-04-02 | Monsanto Technology Llc | Procedimiento para enriquecer una poblacion de celulas de esperma. |
US7355696B2 (en) | 2005-02-01 | 2008-04-08 | Arryx, Inc | Method and apparatus for sorting cells |
KR100949923B1 (ko) * | 2008-04-21 | 2010-03-30 | 중앙대학교 산학협력단 | 번식능력이 불량한 수퇘지 선별법 |
US20110236923A1 (en) * | 2010-03-23 | 2011-09-29 | Genetics & Ivf Institute | Method for staining and sorting of a small volume of sperm |
CA2794934A1 (en) * | 2010-04-01 | 2011-10-06 | Inguran, Llc | Methods and systems for reducing dna fragmentation in a processed sperm sample |
CN101793641B (zh) * | 2010-04-14 | 2011-09-28 | 内蒙古蒙牛繁育生物技术股份有限公司 | 一种用于奶牛精子分离的荧光染色方法 |
CN102095675B (zh) * | 2010-11-23 | 2012-05-23 | 内蒙古赛科星繁育生物技术股份有限公司 | 混合奶牛性控冻精产品鉴定和检测方法 |
US9781919B2 (en) | 2011-06-01 | 2017-10-10 | Inguran, Llc | Compositions and methods for improving the quality of processed sperm |
US9347038B2 (en) | 2011-06-01 | 2016-05-24 | Inguran, Llc | Compositions and methods for processing sperm |
US9140688B2 (en) | 2011-09-30 | 2015-09-22 | Inguran, Llc | Sperm staining and sorting methods |
US9888990B2 (en) | 2012-06-06 | 2018-02-13 | Inguran, Llc | Methods for use of sex sorted semen to improve genetic management in swine |
US9433195B2 (en) | 2012-06-06 | 2016-09-06 | Inguran, Llc | Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells |
US10620213B2 (en) | 2012-10-05 | 2020-04-14 | Inguran, Llc | High pressure sperm sorting and flow cytometer methods |
BR112015007480B1 (pt) | 2012-10-05 | 2020-04-22 | Inguran Llc | métodos de separação de esperma com base no sexo de eficácia elevada. |
CN103335934A (zh) * | 2013-06-28 | 2013-10-02 | 浙江星博生物科技有限公司 | 一种基于流式细胞术的精子活率检测试剂 |
CN103344543B (zh) * | 2013-06-28 | 2016-07-06 | 浙江星博生物科技股份有限公司 | 一种基于流式细胞术的精子线粒体膜电位检测试剂 |
CN103555662B (zh) * | 2013-10-31 | 2015-09-16 | 大连金弘基种畜有限公司 | 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离 |
CN104749144A (zh) * | 2013-12-31 | 2015-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | 血细胞检测试剂及血细胞处理方法和识别方法 |
WO2019199853A1 (en) | 2018-04-09 | 2019-10-17 | Inguran, Llc | Methods and compositions for determining the presence or absence of dna aberrations |
US10603075B1 (en) | 2018-11-30 | 2020-03-31 | Ohana Biosciences, Inc. | Compositions and methods for enhancing sperm function |
Family Cites Families (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3005756A (en) * | 1958-11-14 | 1961-10-24 | Noland L Van Demark | Diluter containing carbon dioxide for preserving semen |
US4474875A (en) | 1969-04-10 | 1984-10-02 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
US3791384A (en) | 1971-07-15 | 1974-02-12 | Schaumann H | Artificial insemination of sows |
US4267268A (en) | 1979-03-12 | 1981-05-12 | Nelson Jr Robert A | Spermatozoa extenders |
US4362246A (en) * | 1980-07-14 | 1982-12-07 | Adair Edwin Lloyd | Method of treating collected mammal semen and separating sperm into X Y components |
GB2145112B (en) | 1983-04-27 | 1987-02-18 | Milk Marketing Board | Sorting living spermatozoa |
ES2091823T3 (es) * | 1989-05-10 | 1996-11-16 | Us Agriculture | Metodo para preseleccionar el sexo de la progenie. |
US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
US5798276A (en) * | 1995-06-07 | 1998-08-25 | Molecular Probes, Inc. | Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
US6040139A (en) * | 1995-09-19 | 2000-03-21 | Bova; G. Steven | Laser cell purification system |
DE69529538T2 (de) * | 1995-11-09 | 2003-11-06 | Medical Res Council London | Verwendung von cyb-medium zum transport und lagern von sperma |
US5985216A (en) * | 1997-07-24 | 1999-11-16 | The United States Of America, As Represented By The Secretary Of Agriculture | Flow cytometry nozzle for high efficiency cell sorting |
EP1028622A1 (de) * | 1997-09-22 | 2000-08-23 | University Of Guelph | Verringerung der empfindlichkeit von sperma gegen abkühlung |
US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
US6149867A (en) * | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US6642018B1 (en) | 1998-03-27 | 2003-11-04 | Oncosis Llc | Method for inducing a response in one or more targeted cells |
US6309815B1 (en) | 1998-08-07 | 2001-10-30 | University Of Kansas Medical Center | Composition and method for preparation, storage and activation of large populations of immotile sperm |
AU3146800A (en) | 1999-03-05 | 2000-09-28 | De Danske Kvaegavlsforeninger | Determination of sperm concentration and viability for artificial insemination |
FR2793495B1 (fr) * | 1999-05-14 | 2001-08-10 | Imv Technologies | Dilueur pour la cryoconservation de spermatozoides de bovins |
US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
US6263745B1 (en) * | 1999-12-03 | 2001-07-24 | Xy, Inc. | Flow cytometer nozzle and flow cytometer sample handling methods |
IT1317724B1 (it) * | 2000-01-14 | 2003-07-15 | Istituto Sperimentale Italiano | Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico. |
AR034121A1 (es) | 2000-05-09 | 2004-02-04 | Xy Inc | Metodo para aislar celulas de esperma que contienen cromosoma x de celulas de esperma que contienen cromosoma y |
WO2002019594A2 (en) | 2000-08-02 | 2002-03-07 | Arizona Board Of Regents, Acting On Behalf Of Arizona State University | Scanning fluorescence lifetime microscope: directed evolution |
AU1980202A (en) * | 2000-11-22 | 2002-06-03 | Pharmacia Corporation | Methods and apparatus for producing gender enriched sperm |
AU2002220018A1 (en) | 2000-11-29 | 2002-06-11 | Colorado State University | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
WO2002077011A2 (en) * | 2001-03-12 | 2002-10-03 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Oxidation-reduction sensitive green fluorescent protein variants |
WO2004087177A1 (en) | 2003-03-28 | 2004-10-14 | Monsanto Technology Llc | Process for the staining of sperm |
DE602004024874D1 (de) | 2003-03-28 | 2010-02-11 | Inguran Llc | Eschlechts-sortierten tierspermien |
-
2004
- 2004-03-29 WO PCT/US2004/009903 patent/WO2004087177A1/en active IP Right Grant
- 2004-03-29 JP JP2006509532A patent/JP2006525019A/ja not_active Withdrawn
- 2004-03-29 MX MXPA05010478A patent/MXPA05010478A/es active IP Right Grant
- 2004-03-29 PL PL04758585T patent/PL1608382T3/pl unknown
- 2004-03-29 CN CNB200480014519XA patent/CN100379429C/zh not_active Expired - Lifetime
- 2004-03-29 AU AU2004226343A patent/AU2004226343B2/en not_active Ceased
- 2004-03-29 BR BRPI0408852-2A patent/BRPI0408852A/pt not_active IP Right Cessation
- 2004-03-29 US US10/811,593 patent/US7335507B2/en active Active
- 2004-03-29 CA CA002519989A patent/CA2519989A1/en not_active Abandoned
- 2004-03-29 EP EP04758585A patent/EP1608382B1/de not_active Expired - Lifetime
- 2004-03-29 DE DE602004008241T patent/DE602004008241T2/de not_active Expired - Lifetime
- 2004-03-29 NZ NZ542688A patent/NZ542688A/en unknown
- 2004-03-29 AT AT04758585T patent/ATE369867T1/de not_active IP Right Cessation
-
2005
- 2005-09-27 ZA ZA200507823A patent/ZA200507823B/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2004226343A1 (en) | 2004-10-14 |
AU2004226343B2 (en) | 2008-10-09 |
CN1795004A (zh) | 2006-06-28 |
CA2519989A1 (en) | 2004-10-14 |
EP1608382A1 (de) | 2005-12-28 |
DE602004008241T2 (de) | 2008-05-08 |
NZ542688A (en) | 2008-06-30 |
ATE369867T1 (de) | 2007-09-15 |
BRPI0408852A (pt) | 2006-04-04 |
EP1608382B1 (de) | 2007-08-15 |
JP2006525019A (ja) | 2006-11-09 |
WO2004087177A1 (en) | 2004-10-14 |
ZA200507823B (en) | 2008-03-26 |
US20050003472A1 (en) | 2005-01-06 |
MXPA05010478A (es) | 2005-12-15 |
PL1608382T3 (pl) | 2008-03-31 |
CN100379429C (zh) | 2008-04-09 |
US7335507B2 (en) | 2008-02-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE602004008241D1 (de) | Verfahren zum anfärben von sperma | |
DK1676117T3 (da) | Simplificeret forarbejdning af væv | |
WO2003020877A3 (en) | Staining and sorting genomes and chromosomes using sequence-specific polyamides | |
DE50113731D1 (de) | Verfahren und vorrichtung zum züchten und/oder behandeln von zellen | |
WO2003070898A3 (en) | Selective extraction of dna from groups of cells | |
DE60326603D1 (de) | Verfahren zur klassifizierung und zählung von leukozyten | |
DE602006018595D1 (de) | Verfahren zum anreichern von subpopulationen seltener zellen aus dem blut | |
WO2008099385A3 (en) | Methods and kits for qualifying sperm cells | |
DE602005019156D1 (de) | Verfahren zur auswertung von ribonukleotidsequenzen | |
ATE487767T1 (de) | Marineblau- und schwarzmischungen, verfahren zu ihrer herstellung und ihre verwendung zum färben von hydroxygruppen-haltigen material | |
DE602004026912D1 (de) | Formulierungen und verfahren zum denaturieren von proteinen | |
CY1107864T1 (el) | Φθοριζοντες ανιχνευτες χρωμοσωμικης βαφης | |
HK1096127A1 (en) | Identifying chromosomal abnormalities in cells obtained from follicular fluid | |
Rodríguez-Casuriaga et al. | Rapid preparation of rodent testicular cell suspensions and spermatogenic stages purification by flow cytometry using a novel blue-laser-excitable vital dye | |
DE602004029255D1 (de) | Verfahren zum nachweis von aneuploidie unter verwendung eines multiplex-mikropartikelnachweises | |
ATE456799T1 (de) | Verfahren zum identifizieren von präneoplastischen und neoplastischen stadien in säugern | |
ATE498682T1 (de) | Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten | |
ATE490463T1 (de) | Mehrfarbige partikel | |
ATE502155T1 (de) | Verfahren zum reaktiv-färben von leder, sowie farbstoffe und deren verwendung | |
DK1585836T3 (da) | Fremgangsmåde til bestemmelse af mængden af template-nukleinsyre der forekommer i en pröve | |
ATE317281T1 (de) | Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut | |
WO2006131518A3 (de) | Mischungen roter küpenfarbstoffe, verfahren zu ihrer herstellung und ihre verwendung zum färben von hydroxygruppen-haltigem material | |
DE50213005D1 (de) | Verfahren und vorrichtung zum kultivieren und/oder verteilen von partikeln | |
GB2404383A (en) | Method of detecting DNA variation in sequence data | |
CN1723390B (zh) | 毛细管电泳中的连续样品注射 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8328 | Change in the person/name/address of the agent |
Representative=s name: KADOR & PARTNER, 80469 MUENCHEN |
|
8327 | Change in the person/name/address of the patent owner |
Owner name: INGURAN, LLC (N.D.GES.D. STAATES DELAWARE), NA, US |
|
8364 | No opposition during term of opposition |