DE60139762D1 - Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren - Google Patents

Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren

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Publication number
DE60139762D1
DE60139762D1 DE60139762T DE60139762T DE60139762D1 DE 60139762 D1 DE60139762 D1 DE 60139762D1 DE 60139762 T DE60139762 T DE 60139762T DE 60139762 T DE60139762 T DE 60139762T DE 60139762 D1 DE60139762 D1 DE 60139762D1
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Germany
Prior art keywords
pyrophosphorolysis
pap
allel
sequencing
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE60139762T
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English (en)
Inventor
Qiang Liu
Steve S Sommer
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City of Hope
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City of Hope
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE60139762T 2000-02-23 2001-02-22 Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren Expired - Lifetime DE60139762D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18431500P 2000-02-23 2000-02-23
US18703500P 2000-03-06 2000-03-06
US23718000P 2000-10-03 2000-10-03
PCT/US2001/005527 WO2001062975A2 (en) 2000-02-23 2001-02-22 Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination

Publications (1)

Publication Number Publication Date
DE60139762D1 true DE60139762D1 (de) 2009-10-15

Family

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Family Applications (1)

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DE60139762T Expired - Lifetime DE60139762D1 (de) 2000-02-23 2001-02-22 Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren

Country Status (9)

Country Link
US (2) US6534269B2 (de)
EP (2) EP2112233B1 (de)
JP (1) JP4989004B2 (de)
AT (1) ATE441726T1 (de)
AU (2) AU4162101A (de)
CA (1) CA2395391C (de)
DE (1) DE60139762D1 (de)
ES (1) ES2330408T3 (de)
WO (1) WO2001062975A2 (de)

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GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
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US8304192B2 (en) 2004-03-05 2012-11-06 University Of Toledo Methods and compositions for assessing nucleic acids and alleles
EP1745157A4 (de) * 2004-04-12 2008-06-11 Ohio Med College Verfahren und zusammensetzungen zum testen von analyten
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
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EP2215222B1 (de) * 2007-11-28 2015-09-16 Roche Diagnostics GmbH Mutante dna-polymerasen mit verbesserter fähigkeit zur pyrophosphorolyse-aktivierten polymerisierung (pap)
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
AU2009242546B2 (en) 2008-04-30 2015-01-22 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
CN101381766B (zh) * 2008-06-11 2012-04-18 厦门大学 一种基因稀有突变的定量检测方法
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
US8481685B2 (en) * 2008-11-03 2013-07-09 Kapa Biosystems Modified DNA polymerases
EP2652152A4 (de) * 2010-12-13 2014-06-25 Life Technologies Corp Polymerisation von nukleinsäuren mittels aktivierung durch polyphosphorolyse-reaktionen (app)
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
CN108496260B (zh) 2015-10-26 2020-05-19 Oti照明公司 用于图案化表面上覆层的方法和包括图案化覆层的装置
CN105316421B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 用于检测与肺癌相关的shox2基因启动子区甲基化程度的试剂盒
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
US11581487B2 (en) 2017-04-26 2023-02-14 Oti Lumionics Inc. Patterned conductive coating for surface of an opto-electronic device
JP7264488B2 (ja) 2017-05-17 2023-04-25 オーティーアイ ルミオニクス インコーポレーテッド パターン化コーティングにわたって伝導性コーティングを選択的に堆積させるための方法および伝導性コーティングを含むデバイス
WO2019062614A1 (en) * 2017-09-26 2019-04-04 Phocus Technology ( Shanghai) Co. Ltd METHOD FOR AMPLIFYING TARGET NUCLEIC ACID
US11751415B2 (en) 2018-02-02 2023-09-05 Oti Lumionics Inc. Materials for forming a nucleation-inhibiting coating and devices incorporating same
US20210032609A1 (en) * 2018-03-21 2021-02-04 Roche Molecular Systems, Inc. Dna polymerases for efficient and effective incorporation of methylated-dntps
US11268140B2 (en) * 2018-06-12 2022-03-08 Shaofeng Ding Delayed pyrophosphorolysis activated polymerization
WO2020178804A1 (en) 2019-03-07 2020-09-10 Oti Lumionics Inc. Materials for forming a nucleation-inhibiting coating and devices incorporating same
US11832473B2 (en) 2019-06-26 2023-11-28 Oti Lumionics Inc. Optoelectronic device including light transmissive regions, with light diffraction characteristics
JP7386556B2 (ja) 2019-06-26 2023-11-27 オーティーアイ ルミオニクス インコーポレーテッド 光回折特性に関連する用途を備えた光透過領域を含む光電子デバイス
JP2022544198A (ja) 2019-08-09 2022-10-17 オーティーアイ ルミオニクス インコーポレーテッド 補助電極および仕切りを含む光電子デバイス

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DE69118930T2 (de) * 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
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US6335162B1 (en) 1998-03-13 2002-01-01 Promega Corporation Nucleic acid detection
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Also Published As

Publication number Publication date
CA2395391A1 (en) 2001-08-30
EP2112233A1 (de) 2009-10-28
WO2001062975A2 (en) 2001-08-30
US20030092051A1 (en) 2003-05-15
JP4989004B2 (ja) 2012-08-01
EP2112233B1 (de) 2013-10-23
EP1259646A2 (de) 2002-11-27
US7105298B2 (en) 2006-09-12
AU4162101A (en) 2001-09-03
WO2001062975A3 (en) 2002-08-29
ATE441726T1 (de) 2009-09-15
ES2330408T3 (es) 2009-12-10
JP2003526344A (ja) 2003-09-09
US6534269B2 (en) 2003-03-18
WO2001062975A9 (en) 2002-10-24
AU2001241621B2 (en) 2005-08-25
CA2395391C (en) 2011-08-02
US20020127554A1 (en) 2002-09-12
EP1259646B1 (de) 2009-09-02

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