DE60139762D1 - Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren - Google Patents

Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren

Info

Publication number
DE60139762D1
DE60139762D1 DE60139762T DE60139762T DE60139762D1 DE 60139762 D1 DE60139762 D1 DE 60139762D1 DE 60139762 T DE60139762 T DE 60139762T DE 60139762 T DE60139762 T DE 60139762T DE 60139762 D1 DE60139762 D1 DE 60139762D1
Authority
DE
Germany
Prior art keywords
pyrophosphorolysis
pap
allel
sequencing
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60139762T
Other languages
English (en)
Inventor
Qiang Liu
Steve S Sommer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
City of Hope
Original Assignee
City of Hope
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by City of Hope filed Critical City of Hope
Application granted granted Critical
Publication of DE60139762D1 publication Critical patent/DE60139762D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE60139762T 2000-02-23 2001-02-22 Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren Expired - Lifetime DE60139762D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18431500P 2000-02-23 2000-02-23
US18703500P 2000-03-06 2000-03-06
US23718000P 2000-10-03 2000-10-03
PCT/US2001/005527 WO2001062975A2 (en) 2000-02-23 2001-02-22 Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination

Publications (1)

Publication Number Publication Date
DE60139762D1 true DE60139762D1 (de) 2009-10-15

Family

ID=27391815

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60139762T Expired - Lifetime DE60139762D1 (de) 2000-02-23 2001-02-22 Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren

Country Status (9)

Country Link
US (2) US6534269B2 (de)
EP (2) EP1259646B1 (de)
JP (1) JP4989004B2 (de)
AT (1) ATE441726T1 (de)
AU (2) AU4162101A (de)
CA (1) CA2395391C (de)
DE (1) DE60139762D1 (de)
ES (1) ES2330408T3 (de)
WO (1) WO2001062975A2 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
AUPR050700A0 (en) * 2000-10-03 2000-10-26 Id+Plus Ltd Detection method
AU2001291513B2 (en) * 2000-10-03 2007-06-07 Id+Plus Ltd Nucleotide detection method
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6777189B2 (en) * 2001-03-30 2004-08-17 Applera Corporation Nucleic acid analysis using non-templated nucleotide addition
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
EP1415004A4 (de) * 2001-07-03 2005-01-19 Univ Leland Stanford Junior Biolumineszenz regenerierender kreislauf (brc) zur nukleinsäurequantifizierung
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
WO2003095664A2 (en) * 2002-05-10 2003-11-20 City Of Hope Pyrophosphorolysis activated polymerization (pap)
EP1753873A4 (de) 2004-03-05 2008-11-05 Ohio Med College Verfahren und zusammensetzungen zur beurteilung von nukleinsäuren und allelen
CA2568238C (en) 2004-04-12 2013-10-15 Medical College Of Ohio Methods and compositions for assaying analytes
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US20060060977A1 (en) * 2004-09-22 2006-03-23 Kabushiki Kaisha Toshiba Semiconductor device
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
WO2009068268A1 (en) * 2007-11-28 2009-06-04 Roche Diagnostics Gmbh Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CN101381766B (zh) * 2008-06-11 2012-04-18 厦门大学 一种基因稀有突变的定量检测方法
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
WO2010062779A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified dna polymerases
EP2652152A4 (de) * 2010-12-13 2014-06-25 Life Technologies Corp Polymerisation von nukleinsäuren mittels aktivierung durch polyphosphorolyse-reaktionen (app)
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CN111628101A (zh) 2015-10-26 2020-09-04 Oti照明公司 用于图案化表面上覆层的方法和包括图案化覆层的装置
CN105316421B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 用于检测与肺癌相关的shox2基因启动子区甲基化程度的试剂盒
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
CN110785867B (zh) 2017-04-26 2023-05-02 Oti照明公司 用于图案化表面上覆层的方法和包括图案化覆层的装置
CN110832660B (zh) 2017-05-17 2023-07-28 Oti照明公司 在图案化涂层上选择性沉积传导性涂层的方法和包括传导性涂层的装置
CN110603326B (zh) * 2017-09-26 2024-03-08 宁波尚宁生物技术有限公司 扩增靶核酸的方法
US11751415B2 (en) 2018-02-02 2023-09-05 Oti Lumionics Inc. Materials for forming a nucleation-inhibiting coating and devices incorporating same
CN111868238A (zh) * 2018-03-21 2020-10-30 豪夫迈·罗氏有限公司 用于甲基化-dNTPs的效率高的和有效参入的DNA聚合酶
US11268140B2 (en) * 2018-06-12 2022-03-08 Shaofeng Ding Delayed pyrophosphorolysis activated polymerization
KR20210149058A (ko) 2019-03-07 2021-12-08 오티아이 루미오닉스 인크. 핵생성 억제 코팅물 형성용 재료 및 이를 포함하는 디바이스
US11832473B2 (en) 2019-06-26 2023-11-28 Oti Lumionics Inc. Optoelectronic device including light transmissive regions, with light diffraction characteristics
KR20220046551A (ko) 2019-06-26 2022-04-14 오티아이 루미오닉스 인크. 광 회절 특성을 갖는 광 투과 영역을 포함하는 광전자 디바이스
JP2022544198A (ja) 2019-08-09 2022-10-17 オーティーアイ ルミオニクス インコーポレーテッド 補助電極および仕切りを含む光電子デバイス

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0439182B1 (de) * 1990-01-26 1996-04-24 Abbott Laboratories Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6159693A (en) * 1998-03-13 2000-12-12 Promega Corporation Nucleic acid detection
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270974B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6270973B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6335162B1 (en) * 1998-03-13 2002-01-01 Promega Corporation Nucleic acid detection
US6277578B1 (en) * 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6312902B1 (en) * 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
WO2001075139A1 (en) * 2000-04-03 2001-10-11 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization

Also Published As

Publication number Publication date
US7105298B2 (en) 2006-09-12
WO2001062975A2 (en) 2001-08-30
EP1259646A2 (de) 2002-11-27
ES2330408T3 (es) 2009-12-10
JP2003526344A (ja) 2003-09-09
CA2395391C (en) 2011-08-02
EP2112233A1 (de) 2009-10-28
ATE441726T1 (de) 2009-09-15
WO2001062975A3 (en) 2002-08-29
JP4989004B2 (ja) 2012-08-01
US6534269B2 (en) 2003-03-18
EP1259646B1 (de) 2009-09-02
WO2001062975A9 (en) 2002-10-24
AU4162101A (en) 2001-09-03
US20030092051A1 (en) 2003-05-15
AU2001241621B2 (en) 2005-08-25
US20020127554A1 (en) 2002-09-12
CA2395391A1 (en) 2001-08-30
EP2112233B1 (de) 2013-10-23

Similar Documents

Publication Publication Date Title
DE60139762D1 (de) Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren
DK0676476T3 (da) Isotermisk nukleinsyreamplificering ved strengdeplacering
ATE262595T1 (de) Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
DE69627698D1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
ATE227347T1 (de) Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung
ATE364718T1 (de) Verfahren zur vervielfältigung von nukleinsäure
DK1040202T3 (da) DNA-polymorfiidentitetsbestemmelse under anvendelse af flowcytometri
ATE184321T1 (de) Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen
DE69725076D1 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
CA2189941A1 (en) Fluorescence Polarization Detection of Nucleic Acids
DE69936532D1 (de) Verfahren zum quantitativen nachweis von nukleinsäuren
NO20005428L (no) Varmelabil uracil-DNA-glykosylase, samt anvendelse derav
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE69521756T2 (de) Verfahren für die Einordnung von Gerste- und Malzsorten mittels Gendiagnose und ein dafür bestimmter Primer
AU783309C (en) Oligonucleotide primers for efficient reverse transcription of hepatitis C virus (HCV) RNA and methods of use thereof
ATE318617T1 (de) Synthetische oligonukleotide als erythroid differenzierungserreger
DK0714694T3 (da) Slange til beluftning af flydende medier
WO2001098475A3 (de) Verfahren zum nachweis von cathepsinen, asparaginylendopeptidase und deren isozyme, sowie von leukocystatin
WO2002083939A3 (de) Steuerbare verstärkung der empfindlichkeit beim hybridisierungsnachweis

Legal Events

Date Code Title Description
8364 No opposition during term of opposition