DE60115958D1 - Zwillingsarginin translokation in bacillus - Google Patents

Zwillingsarginin translokation in bacillus

Info

Publication number
DE60115958D1
DE60115958D1 DE60115958T DE60115958T DE60115958D1 DE 60115958 D1 DE60115958 D1 DE 60115958D1 DE 60115958 T DE60115958 T DE 60115958T DE 60115958 T DE60115958 T DE 60115958T DE 60115958 D1 DE60115958 D1 DE 60115958D1
Authority
DE
Germany
Prior art keywords
gram
host cell
protein
bacillus
secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60115958T
Other languages
English (en)
Other versions
DE60115958T2 (de
Inventor
Douwe Jongbloed
Paul Mueller
Dijl Maarten Van
Sierd Bron
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of DE60115958D1 publication Critical patent/DE60115958D1/de
Application granted granted Critical
Publication of DE60115958T2 publication Critical patent/DE60115958T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
DE60115958T 2000-09-18 2001-09-17 Zwillingsarginin translokation in bacillus Expired - Lifetime DE60115958T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23361000P 2000-09-18 2000-09-18
US233610P 2000-09-18
PCT/US2001/029151 WO2002022667A2 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus

Publications (2)

Publication Number Publication Date
DE60115958D1 true DE60115958D1 (en) 2006-01-19
DE60115958T2 DE60115958T2 (de) 2006-08-03

Family

ID=22877967

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60115958T Expired - Lifetime DE60115958T2 (de) 2000-09-18 2001-09-17 Zwillingsarginin translokation in bacillus

Country Status (7)

Country Link
US (4) US7316924B2 (de)
EP (1) EP1356060B1 (de)
AT (1) ATE312932T1 (de)
AU (1) AU2001292749A1 (de)
DE (1) DE60115958T2 (de)
DK (1) DK1356060T3 (de)
WO (1) WO2002022667A2 (de)

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US7901693B2 (en) * 1998-09-30 2011-03-08 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7329410B1 (en) 1998-09-30 2008-02-12 Corixa Corporation Compositions and method for WT1 specific immunotherapy
US7655249B2 (en) * 1998-09-30 2010-02-02 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7553494B2 (en) 2001-08-24 2009-06-30 Corixa Corporation WT1 fusion proteins
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
EP1451367A4 (de) * 2001-11-05 2006-06-14 Res Dev Foundation Engineering von leader-peptiden für die sekretion von rekombinanten proteinen in bakterien
AU2003220493B2 (en) * 2002-03-23 2008-06-05 Research Development Foundation Secretion of proteins with multiple disulfide bonds in bacteria and uses thereof
DE10256669B4 (de) * 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Gemisch mindestens zweier Fusionsproteine sowie ihre Herstellung und Verwendung
EP1748077B1 (de) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Verfahren zur proteinherstellung
US20070026012A1 (en) * 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
EP2360473A1 (de) * 2005-08-22 2011-08-24 Cornell Research Foundation Zusammensetzungen und Verfahren zur Analyse von Proteininteraktionen
KR100742218B1 (ko) 2005-10-21 2007-07-24 학교법인 포항공과대학교 트윈 아르기닌 트랜스로케이션 분비경로를 가지는오가노포스포러스 하이드롤라제 분비서열을 이용한대장균에서의 목적 외래 단백질 분비
WO2008051491A2 (en) 2006-10-20 2008-05-02 Danisco Us, Inc. Genencor Division Polyol oxidases
US8227227B2 (en) * 2006-11-30 2012-07-24 Novozymes A/S DNase expression in recombinant host cells
US8722584B2 (en) * 2007-01-12 2014-05-13 Cornell University Genetic selection for protein folding and solubility in the bacterial periplasm
WO2008156551A2 (en) * 2007-06-18 2008-12-24 Danisco Us, Inc., Genencor Division Tat signal peptides for producing proteins in prokaryotes
DE102008025791A1 (de) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Sekretionsoptimierter Mikroorganismus
DE102008025926A1 (de) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Sekretionsoptimierter Mikroorganismus
EP3044320B1 (de) * 2013-09-11 2020-02-19 Impossible Foods Inc. Ausscheidung hämhaltiger polypeptide
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
PL3294762T3 (pl) 2015-05-11 2022-04-25 Impossible Foods Inc. Konstrukty ekspresyjne i sposoby inżynierii genetycznej drożdży metylotroficznych
KR101791526B1 (ko) * 2016-02-18 2017-11-01 (주)케어젠 발모 촉진 활성을 나타내는 펩타이드 및 이의 용도
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
GB201713732D0 (en) * 2017-08-25 2017-10-11 Alta Innovations Ltd Tat expression system
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
SG11202111693YA (en) 2019-04-25 2021-11-29 Impossible Foods Inc Strains and methods for production of heme-containing proteins
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

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NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
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IE910663A1 (en) 1990-02-28 1991-08-28 Gist Brocades Nv Improved processing of proteins
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
CA2324974A1 (en) * 1998-04-01 1999-10-14 The Governors Of The University Of Alberta Compositions and methods for protein secretion
AU2002243205A1 (en) 2000-10-10 2002-07-24 Genencor International, Inc. Enhanced secretion of a polypeptide by a microorganism

Also Published As

Publication number Publication date
WO2002022667A3 (en) 2003-09-04
WO2002022667A9 (en) 2003-03-20
US7316924B2 (en) 2008-01-08
EP1356060B1 (de) 2005-12-14
US20020110860A1 (en) 2002-08-15
US20090093025A1 (en) 2009-04-09
US7884191B2 (en) 2011-02-08
DE60115958T2 (de) 2006-08-03
US7897742B2 (en) 2011-03-01
US7884192B2 (en) 2011-02-08
EP1356060A2 (de) 2003-10-29
DK1356060T3 (da) 2006-05-01
ATE312932T1 (de) 2005-12-15
WO2002022667A2 (en) 2002-03-21
US20080166757A1 (en) 2008-07-10
AU2001292749A1 (en) 2002-03-26
US20080248525A1 (en) 2008-10-09

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