DE60044490D1 - Manipulation von mikroteilchen in mikrofluiden systemen - Google Patents

Manipulation von mikroteilchen in mikrofluiden systemen

Info

Publication number
DE60044490D1
DE60044490D1 DE60044490T DE60044490T DE60044490D1 DE 60044490 D1 DE60044490 D1 DE 60044490D1 DE 60044490 T DE60044490 T DE 60044490T DE 60044490 T DE60044490 T DE 60044490T DE 60044490 D1 DE60044490 D1 DE 60044490D1
Authority
DE
Germany
Prior art keywords
microteils
manipulation
systems
microfluidic
arrays
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60044490T
Other languages
English (en)
Inventor
Mehta Tammy Burd
Anne R Kopf-Sill
Wallace J Parce
Andrea W Chow
Luc J Bousse
Michael R Knapp
Theo T Nikiforov
Steve Gallagher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27382592&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60044490(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Caliper Life Sciences Inc filed Critical Caliper Life Sciences Inc
Application granted granted Critical
Publication of DE60044490D1 publication Critical patent/DE60044490D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B01J2219/00468—Beads by manipulation of individual beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00564—Handling or washing solid phase elements, e.g. beads
    • G01N2035/00574—Means for distributing beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE60044490T 1999-02-23 2000-02-22 Manipulation von mikroteilchen in mikrofluiden systemen Expired - Lifetime DE60044490D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12122399P 1999-02-23 1999-02-23
US12782599P 1999-04-05 1999-04-05
US12864399P 1999-04-09 1999-04-09
PCT/US2000/004522 WO2000050172A1 (en) 1999-02-23 2000-02-22 Manipulation of microparticles in microfluidic systems

Publications (1)

Publication Number Publication Date
DE60044490D1 true DE60044490D1 (de) 2010-07-15

Family

ID=27382592

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60044490T Expired - Lifetime DE60044490D1 (de) 1999-02-23 2000-02-22 Manipulation von mikroteilchen in mikrofluiden systemen
DE60045917T Expired - Lifetime DE60045917D1 (de) 1999-02-23 2000-02-22 Sequenzierung durch inkorporation

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE60045917T Expired - Lifetime DE60045917D1 (de) 1999-02-23 2000-02-22 Sequenzierung durch inkorporation

Country Status (6)

Country Link
US (10) US6613513B1 (de)
EP (3) EP2177627B1 (de)
AT (3) ATE556149T1 (de)
AU (2) AU3498900A (de)
DE (2) DE60044490D1 (de)
WO (2) WO2000050642A1 (de)

Families Citing this family (775)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
DE69825601T2 (de) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7079241B2 (en) * 2000-04-06 2006-07-18 Invitrogen Corp. Spatial positioning of spectrally labeled beads
DE60044490D1 (de) * 1999-02-23 2010-07-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2000070080A1 (en) 1999-05-17 2000-11-23 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) * 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
ATE382858T1 (de) * 2000-02-23 2008-01-15 Caliper Life Sciences Inc Mehrfach-reservoir-drucksteuersystem
US20020012971A1 (en) 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
DE10015448A1 (de) * 2000-03-29 2001-10-11 November Ag Molekulare Medizin Verfahren zum Nachweisen und/oder Quantifizieren des Bindens erster Moleküle an dazu affine zweite Moleküle
WO2001073121A1 (fr) * 2000-03-30 2001-10-04 Toyota Jidosha Kabushiki Kaisha Procede pour determiner une sequence de base d'une molecule individuelle d'acide nucleique
EP1161985B1 (de) * 2000-06-05 2005-10-26 STMicroelectronics S.r.l. Verfahren zur Herstellung integrierter chemischer Mikroreaktoren aus Halbleitermaterial sowie integrierter Mikroreaktor
US9709559B2 (en) * 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
US6829753B2 (en) * 2000-06-27 2004-12-07 Fluidigm Corporation Microfluidic design automation method and system
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
GB0022069D0 (en) * 2000-09-08 2000-10-25 Pyrosequencing Ab Method
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP1790736A3 (de) 2000-10-06 2007-08-15 The Trustees Of Columbia University In The City Of New York Massives paralleles Verfahren zum Entschlüsseln von DNA und RNA
AU1189702A (en) * 2000-10-13 2002-04-22 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
US7105304B1 (en) 2000-11-07 2006-09-12 Caliper Life Sciences, Inc. Pressure-based mobility shift assays
US20090118139A1 (en) 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
WO2002038806A2 (de) * 2000-11-13 2002-05-16 Gnothis Holding Sa Nachweis von nukleinsäure-polymorphismen
FR2817343B1 (fr) * 2000-11-29 2003-05-09 Commissariat Energie Atomique Procede et dispositifs de transport et de concentration d'un analyte present dans un echantillon
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7691645B2 (en) * 2001-01-09 2010-04-06 Agilent Technologies, Inc. Immunosubtraction method
US20020110835A1 (en) * 2001-02-13 2002-08-15 Rajan Kumar Microfluidic devices and methods
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7297518B2 (en) * 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
JP4199544B2 (ja) 2001-03-19 2008-12-17 ユィロス・パテント・アクチボラグ マイクロ流体システム（ｅｄｉ）
US6717136B2 (en) 2001-03-19 2004-04-06 Gyros Ab Microfludic system (EDI)
CA2441206A1 (en) 2001-03-19 2002-09-26 Gyros Ab Characterization of reaction variables
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
FR2822848B1 (fr) * 2001-03-28 2004-05-07 Genesystems Dispositif integre et miniature de sequencage d'acides nucleiques
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
DE60227649D1 (de) * 2001-04-03 2008-08-28 Micronics Inc Getrennt fokussierendes zytometer
US20020164816A1 (en) * 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
EP1384022A4 (de) 2001-04-06 2004-08-04 California Inst Of Techn Nukleinsäure-amplifikation verwendende mikrofluidvorrichtungen
US6727479B2 (en) * 2001-04-23 2004-04-27 Stmicroelectronics S.R.L. Integrated device based upon semiconductor technology, in particular chemical microreactor
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
DE10120797B4 (de) * 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
US20020192714A1 (en) * 2001-05-22 2002-12-19 University Of Vermont And State Agricultural College Single polymer matrix unit chromatography
US7172739B2 (en) 2001-05-22 2007-02-06 University Of Vermont And State Agricultural College Protein fractionation
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
HU230352B1 (hu) * 2001-06-12 2016-02-29 Wellstat Therapeutics Corporation Metabolikus rendellenességek kezelésére adható vegyületek és ezeket tartalmazó gyógyászati készítmények
GB0114849D0 (en) * 2001-06-18 2001-08-08 Pig Improvement Co Uk Ltd System
WO2003000404A1 (en) * 2001-06-22 2003-01-03 Domantis Limited Matrix screening method
AU2002319668A1 (en) * 2001-07-27 2003-02-17 President And Fellows Of Harvard College Laminar mixing apparatus and methods
AU2002356030A1 (en) * 2001-08-13 2003-03-03 Vanderbilt University Distribution of solutions across a surface
US7666661B2 (en) 2001-08-27 2010-02-23 Platypus Technologies, Llc Substrates, devices, and methods for quantitative liquid crystal assays
JP4368681B2 (ja) * 2001-09-17 2009-11-18 ユィロス・パテント・アクチボラグ マイクロ流体デバイス内の流れの制御を可能にする機能ユニット
ATE477054T1 (de) 2001-09-17 2010-08-15 Gyros Patent Ab Einen kontrollierten strom in einer mikrofluidvorrichtung ermöglichende funktionseinheit
US6907895B2 (en) * 2001-09-19 2005-06-21 The United States Of America As Represented By The Secretary Of Commerce Method for microfluidic flow manipulation
US6803568B2 (en) * 2001-09-19 2004-10-12 Predicant Biosciences, Inc. Multi-channel microfluidic chip for electrospray ionization
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US20030072682A1 (en) * 2001-10-11 2003-04-17 Dan Kikinis Method and apparatus for performing biochemical testing in a microenvironment
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
AU2002347981A1 (en) 2001-11-07 2003-05-19 Applera Corporation Universal nucleotides for nucleic acid analysis
SE526185C2 (sv) * 2001-11-07 2005-07-19 Prolight Diagnostics Ab Metod och anordning för immunanalys
US7473361B2 (en) * 2001-11-30 2009-01-06 Cornell Research Foundation Diffusion-based molecular separation in structured microfluidic devices
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US20030109067A1 (en) 2001-12-06 2003-06-12 Immunetech, Inc. Homogeneous immunoassays for multiple allergens
WO2003054517A2 (en) * 2001-12-19 2003-07-03 Biogen Idec Ma Inc. Methods for detecting half-antibodies using chip-based gel electrophoresis
US7105810B2 (en) 2001-12-21 2006-09-12 Cornell Research Foundation, Inc. Electrospray emitter for microfluidic channel
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
US20050118647A1 (en) * 2001-12-31 2005-06-02 Yung-Chiang Chung Microfluidic mixer apparatus and microfluidic reactor apparatus for microfluidic processing
US7189370B2 (en) * 2002-02-11 2007-03-13 Microchem Solutions Apparatus and methods for high throughput and high-resolution assays
JP2003232791A (ja) * 2002-02-12 2003-08-22 Olympus Optical Co Ltd プローブ固相化反応アレイ
AU2003219775A1 (en) * 2002-02-15 2003-09-09 President And Fellows Of Harvard College Fluidics systems including magnetic or electric fields and methods of using the same
US7303727B1 (en) 2002-03-06 2007-12-04 Caliper Life Sciences, Inc Microfluidic sample delivery devices, systems, and methods
US7560267B2 (en) * 2002-03-18 2009-07-14 City University Of Hong Kong Apparatus and methods for on-chip monitoring of cellular reactions
DE10214395A1 (de) * 2002-03-30 2003-10-23 Dmitri Tcherkassov Verfahren zur Analyse von Einzelnukleotidpolymorphismen
CA2480728A1 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
DE60301622T2 (de) * 2002-04-04 2006-06-14 Biotage Ab Uppsala Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind
US6808075B2 (en) 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
US8697029B2 (en) * 2002-04-18 2014-04-15 The Regents Of The University Of Michigan Modulated physical and chemical sensors
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
DE60223532T2 (de) * 2002-08-12 2008-03-06 Gnothis Holding Sa Nachweis von Einzelnukleotidpolymorphismen durch Einzelmolekülanalyse
US6916455B2 (en) * 2002-08-14 2005-07-12 The Regents Of The University Of California Protein crystallography prescreen kit
US7872804B2 (en) 2002-08-20 2011-01-18 Illumina, Inc. Encoded particle having a grating with variations in the refractive index
US7901630B2 (en) 2002-08-20 2011-03-08 Illumina, Inc. Diffraction grating-based encoded microparticle assay stick
US7164533B2 (en) 2003-01-22 2007-01-16 Cyvera Corporation Hybrid random bead/chip based microarray
US7923260B2 (en) 2002-08-20 2011-04-12 Illumina, Inc. Method of reading encoded particles
US7508608B2 (en) 2004-11-17 2009-03-24 Illumina, Inc. Lithographically fabricated holographic optical identification element
US7900836B2 (en) 2002-08-20 2011-03-08 Illumina, Inc. Optical reader system for substrates having an optically readable code
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
SI3587433T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi
EP2119722B1 (de) 2002-08-23 2016-10-26 Illumina Cambridge Limited Markierte Nukleotide
US7092160B2 (en) 2002-09-12 2006-08-15 Illumina, Inc. Method of manufacturing of diffraction grating-based optical identification element
US20100255603A9 (en) 2002-09-12 2010-10-07 Putnam Martin A Method and apparatus for aligning microbeads in order to interrogate the same
US6881039B2 (en) * 2002-09-23 2005-04-19 Cooligy, Inc. Micro-fabricated electrokinetic pump
EP1551753A2 (de) 2002-09-25 2005-07-13 California Institute Of Technology Hochintegriertes mikrofluidisches netzwerk
EP1569510B1 (de) 2002-09-27 2011-11-02 The General Hospital Corporation Mikrofluidische vorrichtung zur zelltrennung und verwendungen davon
AU2003265185A1 (en) * 2002-09-27 2004-04-19 Biotage Ab New sequencing method for sequencing rna molecules
JP5695287B2 (ja) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー 微小流体の核酸解析
US7836597B2 (en) 2002-11-01 2010-11-23 Cooligy Inc. Method of fabricating high surface to volume ratio structures and their integration in microheat exchangers for liquid cooling system
US7806168B2 (en) 2002-11-01 2010-10-05 Cooligy Inc Optimal spreader system, device and method for fluid cooled micro-scaled heat exchange
US20060211055A1 (en) * 2002-11-12 2006-09-21 Caliper Life Sciences, Inc. Capture and release assay system and method
KR20040044336A (ko) * 2002-11-12 2004-05-28 마쯔시다덴기산교 가부시키가이샤 특이결합반응 측정방법과 이에 이용하는 시약키트 및특이결합반응 측정장치
US7175810B2 (en) * 2002-11-15 2007-02-13 Eksigent Technologies Processing of particles
WO2004045547A2 (en) * 2002-11-18 2004-06-03 Genospectra, Inc. Rnai-based sensors, caged interfering rnas, and methods of use thereof
CA2506935A1 (en) * 2002-11-20 2004-06-03 University Of Virginia Patent Foundation Isolation of sperm cells from other biological materials using microfabricated devices and related methods thereof
ATE462017T1 (de) * 2002-12-02 2010-04-15 Illumina Cambridge Ltd Bestimmung der methylierung von nukleinsäuresequenzen
US7932025B2 (en) * 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
JP2006512092A (ja) 2002-12-30 2006-04-13 ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア 病原体の検出および分析のための方法および装置
US7332129B2 (en) 2003-01-09 2008-02-19 3M Innovative Properties Company Sample processing device having process chambers with bypass slots
SE0300454D0 (sv) * 2003-02-19 2003-02-19 Aamic Ab Nozzles for electrospray ionization and methods of fabricating them
US8399196B2 (en) 2003-02-21 2013-03-19 Geneform Technologies Limited Nucleic acid sequencing methods, kits and reagents
SE0300822D0 (sv) * 2003-03-23 2003-03-23 Gyros Ab A collection of Micro Scale Devices
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7476363B2 (en) * 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
JP5419248B2 (ja) * 2003-04-03 2014-02-19 フルイディグム コーポレイション マイクロ流体装置およびその使用方法
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7007710B2 (en) * 2003-04-21 2006-03-07 Predicant Biosciences, Inc. Microfluidic devices and methods
EP1636332A4 (de) * 2003-05-06 2007-08-29 Bellbrook Labs Llc Dreidimensionale zellkulturen in einem fluidbearbeitungssystem im mikromassstab
JP4931584B2 (ja) * 2003-07-14 2012-05-16 ウオーターズ・テクノロジーズ・コーポレイシヨン 一体型ガードカラムをもつ分離装置
US7021369B2 (en) * 2003-07-23 2006-04-04 Cooligy, Inc. Hermetic closed loop fluid system
US7591302B1 (en) 2003-07-23 2009-09-22 Cooligy Inc. Pump and fan control concepts in a cooling system
EP2402089A1 (de) 2003-07-31 2012-01-04 Handylab, Inc. Verarbeitung partikelhaltiger Proben
US7413712B2 (en) * 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
GB0320337D0 (en) * 2003-08-29 2003-10-01 Syrris Ltd A microfluidic system
US20050136439A1 (en) * 2003-09-12 2005-06-23 North Carolina State University Novel methods of inorganic compound discovery and synthesis
US7537807B2 (en) 2003-09-26 2009-05-26 Cornell University Scanned source oriented nanofiber formation
WO2005040759A2 (en) * 2003-10-28 2005-05-06 Dakota Technologies, Inc. Apparatus and methods for detecting target analyte
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
CA2545482A1 (en) * 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP4041458B2 (ja) * 2003-12-12 2008-01-30 日立ソフトウエアエンジニアリング株式会社 蛍光ビーズ検出方法及び蛍光ビーズ検出装置
WO2005069015A1 (ja) 2004-01-15 2005-07-28 Japan Science And Technology Agency 化学分析装置及び化学分析方法
JP3991034B2 (ja) * 2004-01-23 2007-10-17 キヤノン株式会社 検出方法
SE0400181D0 (sv) * 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7433123B2 (en) 2004-02-19 2008-10-07 Illumina, Inc. Optical identification element having non-waveguide photosensitive substrate with diffraction grating therein
CN101142314A (zh) * 2004-03-03 2008-03-12 综合医院公司 用于微流体环境中的细胞和生物分子分离的磁力装置
WO2005084367A2 (en) * 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US7442339B2 (en) * 2004-03-31 2008-10-28 Intel Corporation Microfluidic apparatus, Raman spectroscopy systems, and methods for performing molecular reactions
US9863941B2 (en) * 2004-04-01 2018-01-09 Nanyang Technological University Microchip and method for detecting molecules and molecular interactions
RU2252411C1 (ru) * 2004-04-09 2005-05-20 Общество с ограниченной ответственностью "Институт рентгеновской оптики" Флюоресцентный сенсор на основе многоканальных структур
US7297246B2 (en) * 2004-04-22 2007-11-20 Sandia Corporation Electrokinetic pump
EP2345739B8 (de) 2004-05-03 2016-12-07 Handylab, Inc. Mikrofluidische Vorrichtung zur Verarbeitung polynukleotidhaltiger Proben
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
EP2701194B1 (de) 2004-06-07 2018-08-29 Fluidigm Corporation Verfahren und Vorrichtung zur Abbildung eines mikrofluidischen Geräts unter Temperaturkontrolle
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
EP1611955A1 (de) * 2004-07-01 2006-01-04 Agilent Technologies, Inc. Mikrofluidische Vorrichtung mit Filterkanal
JP2006029798A (ja) * 2004-07-12 2006-02-02 Hitachi Software Eng Co Ltd 試薬を内蔵した高反応効率生体物質検査チップ
CA2600899C (en) * 2004-07-16 2014-04-01 Simon Fraser University Microfluidic device and method of using same
WO2006020363A2 (en) 2004-07-21 2006-02-23 Illumina, Inc. Method and apparatus for drug product tracking using encoded optical identification elements
WO2006098752A2 (en) * 2004-07-29 2006-09-21 Kim Laboratories Ultrasensitive sensor and rapid detection of analytes
US20060022130A1 (en) * 2004-07-29 2006-02-02 Predicant Biosciences, Inc., A Delaware Corporation Microfluidic devices and methods with integrated electrical contact
JP4543312B2 (ja) * 2004-08-10 2010-09-15 横河電機株式会社 マイクロリアクタ
DE102004044048A1 (de) * 2004-09-09 2006-03-30 Qualis Laboratorium Gmbh Biochemische Charakterisierung mit zellulären Systemen
EP2261650A3 (de) 2004-09-15 2011-07-06 IntegenX Inc. Mikrofluidische Vorrichtungen
US7745207B2 (en) 2006-02-03 2010-06-29 IntegenX, Inc. Microfluidic devices
US20060060769A1 (en) * 2004-09-21 2006-03-23 Predicant Biosciences, Inc. Electrospray apparatus with an integrated electrode
US7591883B2 (en) 2004-09-27 2009-09-22 Cornell Research Foundation, Inc. Microfiber supported nanofiber membrane
JP4263154B2 (ja) * 2004-09-30 2009-05-13 日立ソフトウエアエンジニアリング株式会社 機能性微粒子アレイ及びその使用方法
DE102004050575B3 (de) * 2004-10-15 2006-01-05 Siemens Ag Verfahren zur kombinierten Isolierung von Magnet-Beads aus einer flüssigen Probe und anschließender Thermozyklisierung für die PCR und zugehörige Anordnung
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
EP2194485B1 (de) 2004-11-16 2012-10-17 Illumina, Inc. Verfahren und Vorrichtung zum Auslesen kodierter Mikroperlen
US20060134772A1 (en) * 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
KR100695134B1 (ko) * 2004-11-25 2007-03-14 삼성전자주식회사 층류를 이용한 마이크로어레이 및 이의 제조 방법
GB0426082D0 (en) * 2004-11-26 2004-12-29 Norchip As A device for carrying out biological assays
US9260693B2 (en) 2004-12-03 2016-02-16 Cytonome/St, Llc Actuation of parallel microfluidic arrays
WO2006074351A2 (en) * 2005-01-05 2006-07-13 Agencourt Personal Genomics Reversible nucleotide terminators and uses thereof
KR100682920B1 (ko) * 2005-01-20 2007-02-15 삼성전자주식회사 다중 바이오어세이용 미세유체 칩 및 그 제조방법
WO2006081479A2 (en) * 2005-01-27 2006-08-03 Applera Corporation Sample preparation devices and methods
PL1859330T3 (pl) 2005-01-28 2013-01-31 Univ Duke Urządzenia i sposoby manipulacji kropelkami na płytkach obwodów drukowanych
CA2593855A1 (en) * 2005-01-31 2006-08-10 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
WO2006084132A2 (en) 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
EP2233582A1 (de) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US9695472B2 (en) * 2005-03-04 2017-07-04 Intel Corporation Sensor arrays and nucleic acid sequencing applications
JP4626350B2 (ja) * 2005-03-22 2011-02-09 ソニー株式会社 ハイブリダイゼーション検出に適する反応部などを有する流路系、該流路系を用いるハイブリダイゼーション検出装置
ES2859774T3 (es) * 2005-04-01 2021-10-04 Lsi Medience Corp Aparato de análisis automático múltiple de muestras biológicas, procedimiento de autoanálisis y cubeta de reacción
US9097723B2 (en) * 2005-04-01 2015-08-04 Caliper Life Sciences, Inc. Method and apparatus for performing peptide digestion on a microfluidic device
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20060223178A1 (en) * 2005-04-05 2006-10-05 Tom Barber Devices and methods for magnetic enrichment of cells and other particles
US20070026418A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
EP2594631A1 (de) * 2005-04-05 2013-05-22 Cellpoint Diagnostics Vorrichtungen und Verfahren zur Nachweisen von zirkulierenden Tumorzellen und anderen Partikeln
US20060246576A1 (en) * 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
JP2006300548A (ja) * 2005-04-15 2006-11-02 Hitachi Software Eng Co Ltd 検査チップ及び検査チップシステム
US20060257907A1 (en) * 2005-04-19 2006-11-16 The Regents Of The University Of California Packed bed for nucleic acid capture and amplification
US20060257958A1 (en) * 2005-05-13 2006-11-16 Pronucleotein Biotechnologies, Llc Magnetically-assisted test strip cartridge and method for using same
DE102005027667A1 (de) * 2005-06-15 2006-12-28 Febit Biotech Gmbh Verfahren zur Qualitätskontrolle funktionalisierter Oberflächen
JP2007010341A (ja) * 2005-06-28 2007-01-18 Sumitomo Bakelite Co Ltd 免疫分析方法
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US9637715B2 (en) 2005-07-07 2017-05-02 Emd Millipore Corporation Cell culture and invasion assay method and system
US9354156B2 (en) 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
US9388374B2 (en) 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
EP1904619A4 (de) 2005-07-07 2012-05-02 Univ California Verfahren und vorrichtung für zellkultur-array
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026419A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US7805081B2 (en) * 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007026963A1 (en) * 2005-08-31 2007-03-08 Nissan Chemical Industries, Ltd. Microchip for cell response evaluation
JP4699840B2 (ja) * 2005-08-31 2011-06-15 ローム株式会社 バイオチップ及び免疫分析方法
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CN101273258A (zh) * 2005-09-30 2008-09-24 卡钳生命科学股份有限公司 利用磁性珠纯化生物学组分的微流体装置
US7763423B2 (en) * 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
US8007267B2 (en) * 2005-11-02 2011-08-30 Affymetrix, Inc. System and method for making lab card by embossing
US20070099288A1 (en) * 2005-11-02 2007-05-03 Affymetrix, Inc. Microfluidic Methods, Devices, and Systems for Fluid Handling
US8075852B2 (en) 2005-11-02 2011-12-13 Affymetrix, Inc. System and method for bubble removal
US20080311585A1 (en) * 2005-11-02 2008-12-18 Affymetrix, Inc. System and method for multiplex liquid handling
US20080038714A1 (en) * 2005-11-02 2008-02-14 Affymetrix, Inc. Instrument to Pneumatically Control Lab Cards and Method Thereof
AU2006318462A1 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
DE102005059536B4 (de) * 2005-12-13 2008-08-28 Siemens Ag Verfahren und Biochip zur Untersuchung einer biologischen Probe
US7913719B2 (en) 2006-01-30 2011-03-29 Cooligy Inc. Tape-wrapped multilayer tubing and methods for making the same
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
SI2385143T1 (sl) * 2006-02-02 2016-11-30 The Board of Trustees of the Leland Stanford Junior University Office of the General Counsel Neinvazivni genetski presejalni test ploda z digitalno analizo
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) * 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
CA2571904A1 (en) * 2006-02-15 2007-08-15 Fio Corporation System and method of detecting pathogens
PL1991680T3 (pl) 2006-03-09 2014-01-31 Scripps Research Inst Układ do ekspresji ortogonalnych składników translacji w eubakteryjnych komórkach gospodarza
EP2007905B1 (de) 2006-03-15 2012-08-22 Micronics, Inc. Integrierte nukleinsäuretests
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2001990B1 (de) 2006-03-24 2016-06-29 Handylab, Inc. Integriertes system zur verarbeitung von mikrofluidischen proben und verwendungsverfahren
US8975216B2 (en) * 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US8157001B2 (en) 2006-03-30 2012-04-17 Cooligy Inc. Integrated liquid to air conduction module
US20080050747A1 (en) * 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
US7563574B2 (en) 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
US9476856B2 (en) * 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8492168B2 (en) * 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US20090062129A1 (en) * 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
WO2009111769A2 (en) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Reagent and sample preparation and loading on a fluidic device
JP4940756B2 (ja) * 2006-05-22 2012-05-30 ソニー株式会社 マイクロ流路系
EP2548972A1 (de) * 2006-06-14 2013-01-23 Verinata Health, Inc Verfahren zur Diagnose fötaler Missbildungen
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US20080070792A1 (en) 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
CA2655018A1 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
KR100824209B1 (ko) * 2006-06-22 2008-04-24 부산대학교 산학협력단 모세관력을 이용한 수동 미세유체 세척 소자
JP2008008692A (ja) * 2006-06-28 2008-01-17 Jeol Ltd マイクロチップ
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
KR100790880B1 (ko) 2006-07-05 2008-01-02 삼성전자주식회사 자성 비드가 결합되어 있는 소수성 다공성 중합체가 벽면에결합되어 있는 마이크로채널 또는 마이크로챔버를포함하는 미세유동장치 및 그를 이용하는 방법
US20080044821A1 (en) * 2006-08-21 2008-02-21 Gafur Zainiev Nucleic acid array having fixed nucleic acid anti-probes and complementary free nucleic acid probes
US7820983B2 (en) * 2006-09-01 2010-10-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US8273310B2 (en) * 2006-09-05 2012-09-25 Samsung Electronics Co., Ltd. Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device
US20080080059A1 (en) * 2006-09-28 2008-04-03 Pacific Biosciences Of California, Inc. Modular optical components and systems incorporating same
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8101403B2 (en) * 2006-10-04 2012-01-24 University Of Washington Method and device for rapid parallel microfluidic molecular affinity assays
KR100799267B1 (ko) * 2006-10-13 2008-01-29 이화여자대학교 산학협력단 Ｎｏａ로 제조한 마이크로 또는 나노 유체칩 및 이를사용하여 제조한 바이오분석 플랫폼
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
EP2091647A2 (de) 2006-11-14 2009-08-26 Handylab, Inc. Mikrofluidisches system für parallele amplifikation und erkennung von polynukleotiden
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
WO2008073378A2 (en) * 2006-12-11 2008-06-19 Thomas Jefferson University Medical College High throughput dna sequencing method and apparatus
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2653861B1 (de) 2006-12-14 2014-08-13 Life Technologies Corporation Verfahren zur Nukleinsäure-Sequenzierung mittels großer FET-Arrays
US8262900B2 (en) * 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US8586385B2 (en) 2006-12-28 2013-11-19 Intel Corporation Method and device for biomolecule preparation and detection using magnetic array
US8409877B2 (en) * 2006-12-29 2013-04-02 Intel Corporation Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
US7993525B2 (en) * 2006-12-29 2011-08-09 Intel Corporation Device and method for particle complex handling
WO2008085991A2 (en) 2007-01-08 2008-07-17 U.S. Genomics, Inc. Reaction chamber
US8617903B2 (en) * 2007-01-29 2013-12-31 The Invention Science Fund I, Llc Methods for allergen detection
US20080180259A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Devices for allergen detection
US20080181816A1 (en) * 2007-01-29 2008-07-31 Searete Llc, A Limited Liability Corporation Systems for allergen detection
US10001496B2 (en) * 2007-01-29 2018-06-19 Gearbox, Llc Systems for allergen detection
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US20110039303A1 (en) 2007-02-05 2011-02-17 Stevan Bogdan Jovanovich Microfluidic and nanofluidic devices, systems, and applications
GB2458855B (en) * 2007-02-05 2011-06-08 Intelligent Bio Systems Inc A system for imaging treated bio-molecules in a flow-cell using a non-lasing light source
DK2111554T3 (da) 2007-02-09 2013-07-22 Advanced Liquid Logic Inc Dråbeaktuatoranordninger og fremgangsmåder til anvendelse af magnetiske korn
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20080220537A1 (en) * 2007-03-07 2008-09-11 Pacific Biosciences Of California, Inc. Substrates and methods for selective immobilization of active molecules
US9068977B2 (en) * 2007-03-09 2015-06-30 The Regents Of The University Of Michigan Non-linear rotation rates of remotely driven particles and uses thereof
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US20080234143A1 (en) * 2007-03-23 2008-09-25 Pacific Biosciences Of California, Inc. Mechanical methods for producing substrates having selectively immobilized molecules
US8360321B2 (en) 2007-04-02 2013-01-29 Fio Corporation System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
CN101680889A (zh) * 2007-05-08 2010-03-24 霍夫曼-拉罗奇有限公司 用于检测特异性免疫球蛋白g类抗体的方法
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
US20100167413A1 (en) * 2007-05-10 2010-07-01 Paul Lundquist Methods and systems for analyzing fluorescent materials with reduced autofluorescence
WO2009000084A1 (en) 2007-06-22 2008-12-31 Fio Corporation Systems and methods for manufacturing quantum dot-doped polymer microbeads
WO2009006739A1 (en) 2007-07-09 2009-01-15 Fio Corporation Systems and methods for enhancing fluorescent detection of target molecules in a test sample
EP3741869A1 (de) 2007-07-13 2020-11-25 Handylab, Inc. Materialien zur erfassung von polynukleotiden und verfahren zur verwendung davon
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8016260B2 (en) 2007-07-19 2011-09-13 Formulatrix, Inc. Metering assembly and method of dispensing fluid
PT2183693E (pt) 2007-07-23 2014-01-14 Univ Hong Kong Chinese Diagnóstico de aneuploidia cromossómica fetal utilizando sequenciação genómica
US20100112590A1 (en) 2007-07-23 2010-05-06 The Chinese University Of Hong Kong Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
CA2693979A1 (en) * 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
TW200934352A (en) 2007-08-07 2009-08-01 Cooligy Inc Internal access mechanism for a server rack
US20110290330A1 (en) * 2007-08-09 2011-12-01 Agilent Technologies, Inc. Fluid flow control in a microfluidic device
US9968935B2 (en) 2007-08-20 2018-05-15 Platypus Technologies, Llc Devices for cell assays
JP2010537643A (ja) * 2007-08-29 2010-12-09 アプライド バイオシステムズ， エルエルシー 代替的な核酸配列決定法
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
JP5227556B2 (ja) * 2007-09-06 2013-07-03 株式会社日立製作所 分析装置
WO2009034563A2 (en) * 2007-09-14 2009-03-19 Nanocomms Patents Limited An analysis system
US7960116B2 (en) * 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
WO2009045344A2 (en) * 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
US20090085427A1 (en) * 2007-10-01 2009-04-02 The Regents Of The University Of Michigan Electrical power generation from fluid flow
US20090191553A1 (en) * 2007-10-01 2009-07-30 Applied Biosystems Inc. Chase Ligation Sequencing
US8551763B2 (en) 2007-10-12 2013-10-08 Fio Corporation Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto
US8789536B2 (en) 2007-10-17 2014-07-29 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8303573B2 (en) 2007-10-17 2012-11-06 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8707964B2 (en) * 2007-10-31 2014-04-29 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US20090105561A1 (en) * 2007-10-17 2009-04-23 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Medical or veterinary digestive tract utilization systems and methods
EP2725107B1 (de) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA-Sequenzierung mit nichtfluoreszierenden, reversiblen Nukleotidterminatoren und spaltbaren, fluoreszierenden ddNTPs und Nukleinsäuren die Inosine mit reversiblen Terminatorgruppe
WO2009051807A1 (en) 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US8808276B2 (en) * 2007-10-23 2014-08-19 The Invention Science Fund I, Llc Adaptive dispensation in a digestive tract
US20090130746A1 (en) * 2007-10-25 2009-05-21 Canon U.S. Life Sciences, Inc. Microchannel surface coating
US20090163894A1 (en) * 2007-10-31 2009-06-25 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Medical or veterinary digestive tract utilization systems and methods
US8808271B2 (en) * 2007-10-31 2014-08-19 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8333754B2 (en) * 2007-10-31 2012-12-18 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US8109920B2 (en) * 2007-10-31 2012-02-07 The Invention Science Fund I, Llc Medical or veterinary digestive tract utilization systems and methods
US9211537B2 (en) 2007-11-07 2015-12-15 The University Of British Columbia Microfluidic device and method of using same
US8049183B1 (en) * 2007-11-09 2011-11-01 Carnegie Mellon University Apparatuses and methods for control and self-assembly of particles into adaptable monolayers
US9975118B2 (en) * 2007-11-15 2018-05-22 Seng Enterprises Ltd. Device for the study of living cells
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
WO2009067632A1 (en) * 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
US20090137866A1 (en) * 2007-11-28 2009-05-28 Searete Llc, A Limited Liability Corporation Of The State Delaware Medical or veterinary digestive tract utilization systems and methods
US20090155840A1 (en) * 2007-12-17 2009-06-18 Beebe David J Method and device for cell counting
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
WO2009086403A2 (en) 2007-12-23 2009-07-09 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods of conducting droplet operations
WO2009086353A1 (en) * 2007-12-26 2009-07-09 Helicos Biosciences Corporation Improved two-primer sequencing for high-throughput expression analysis
US8815576B2 (en) * 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
WO2009089189A2 (en) 2008-01-03 2009-07-16 Cellasic Cell culture array system for automated assays and methods of operation and manufacture thereof
AU2009204461A1 (en) 2008-01-10 2009-07-16 Pacific Biosciences Of California, Inc. Methods and systems for analysis of fluorescent reactions with modulated excitation
CN101990516B (zh) 2008-01-22 2015-09-09 英特基因有限公司 多用试样准备系统及其在集成分析系统中的使用
US8252911B2 (en) 2008-02-12 2012-08-28 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US8741815B2 (en) * 2008-02-19 2014-06-03 Intelligent Bio Systems, Inc. Methods and devices for amplification of nucleic acid
US8277759B2 (en) * 2008-03-04 2012-10-02 The University Of Utah Research Foundation Microfluidic flow cell
US9297571B1 (en) 2008-03-10 2016-03-29 Liebert Corporation Device and methodology for the removal of heat from an equipment rack by means of heat exchangers mounted to a door
US8632243B2 (en) * 2008-03-10 2014-01-21 The Hong Kong Polytechnic University Microfluidic mixing using continuous acceleration/deceleration methodology
US8250877B2 (en) 2008-03-10 2012-08-28 Cooligy Inc. Device and methodology for the removal of heat from an equipment rack by means of heat exchangers mounted to a door
US8236499B2 (en) * 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
CN104862383B (zh) 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
US20090269746A1 (en) * 2008-04-25 2009-10-29 Gil Atzmon Microsequencer-whole genome sequencer
WO2009137415A2 (en) 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
TWI460602B (zh) * 2008-05-16 2014-11-11 Counsyl Inc 廣用的懷孕前篩檢裝置
WO2009150583A1 (en) * 2008-06-10 2009-12-17 Koninklijke Philips Electronics N.V. Diagnostic device
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
JP5667049B2 (ja) 2008-06-25 2015-02-12 ライフ テクノロジーズ コーポレーション 大規模なｆｅｔアレイを用いて分析物を測定するための方法および装置
WO2009155704A1 (en) 2008-06-25 2009-12-30 Fio Corporation Bio-threat alert system
US20090325159A1 (en) * 2008-06-30 2009-12-31 Canon U.S. Life Sciences, Inc. System and method to prevent cross-contamination in assays performed in a microfluidic channel
WO2010002939A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Methods for real time single molecule sequencing
WO2010003153A2 (en) * 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US8198023B2 (en) * 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
JP5597633B2 (ja) * 2008-08-15 2014-10-01 コーネル ユニバーシティ 化学的ターゲットと特異的に結合する核酸の迅速な確認のための装置
GB0815212D0 (en) 2008-08-20 2008-09-24 Roar Particles Ltd Identification of sample components
WO2010022519A1 (en) 2008-08-29 2010-03-04 Fio Corporation A single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples
WO2010027497A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
DK3629011T3 (da) 2008-09-16 2024-01-29 Pacific Biosciences California Inc Integreret optisk indretning
US20110218123A1 (en) * 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8481264B2 (en) * 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8383369B2 (en) * 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8361716B2 (en) 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
CN102170971B (zh) * 2008-10-06 2013-12-11 皇家飞利浦电子股份有限公司 微流体装置
CA2740113C (en) * 2008-10-10 2019-12-24 The Governing Council Of The University Of Toronto Hybrid digital and channel microfluidic devices and methods of use thereof
EP2350652A2 (de) * 2008-10-10 2011-08-03 Cnrs-Dae Zellsortierungsvorrichtung
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8252910B2 (en) * 2008-11-19 2012-08-28 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
US9175338B2 (en) * 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
EP2370598B1 (de) * 2008-12-11 2017-02-15 Pacific Biosciences Of California, Inc. Klassifizierung von nukleinsäuretemplaten
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
US8236532B2 (en) * 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
CA2748364C (en) 2008-12-25 2018-04-17 Universal Bio Research Co., Ltd. Method for pretreating specimen and method for assaying biological substance
WO2010077322A1 (en) 2008-12-31 2010-07-08 Microchip Biotechnologies, Inc. Instrument with microfluidic chip
EP2387721A4 (de) 2009-01-13 2014-05-14 Fio Corp Tragbare diagnosetestvorrichtung und verfahren zu deren verwendung mit einer elektronischen vorrichtung sowie testkartusche für schnelle diagnosetests
US8100293B2 (en) 2009-01-23 2012-01-24 Formulatrix, Inc. Microfluidic dispensing assembly
DE102009005925B4 (de) 2009-01-23 2013-04-04 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Vorrichtung und Verfahren zur Handhabung von Biomolekülen
US8569046B2 (en) * 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels
WO2010122990A1 (ja) 2009-04-20 2010-10-28 ユニバーサル・バイオ・リサーチ株式会社 生体関連物質測定用チューブおよび定量システム
US20100270160A1 (en) * 2009-04-22 2010-10-28 Naf Technologies Llc Portable Low Power Charged Particle Analysis Device
US20100330569A1 (en) * 2009-04-23 2010-12-30 Intelligent Bio-Systems, Inc. Hydroxymethyl Linkers For Labeling Nucleotides
WO2010129019A2 (en) 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
DE102009040151B4 (de) * 2009-05-26 2013-09-12 Analytik Jena Ag Anordnung zur Detektion von Chemolumineszenz an Gasen
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
WO2010138960A2 (en) * 2009-05-29 2010-12-02 President And Fellows Of Harvard College Methods and systems for single-molecule rna expression profiling
EP2438154A1 (de) 2009-06-02 2012-04-11 Integenx Inc. Fluidische vorrichtung mit membranventilen
DE112010002222B4 (de) 2009-06-04 2024-01-25 Leidos Innovations Technology, Inc. (n.d.Ges.d. Staates Delaware) Mehr-Proben-Mikrofluidchip fur DNA-Analyse
AU2010256429B2 (en) 2009-06-05 2015-09-17 Integenx Inc. Universal sample preparation system and use in an integrated analysis system
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
DK2456892T3 (en) * 2009-07-24 2014-12-08 Illumina Inc Procedure for sequencing of a polynukleotidskabelon
CN102712950B (zh) * 2009-07-29 2015-05-20 皮罗比特私人有限公司 用于实施测定法的方法和装置
DE102009035941B8 (de) * 2009-08-03 2017-04-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Diagnostiksystem
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
WO2011026136A1 (en) * 2009-08-31 2011-03-03 Life Technologies Corporation Low-volume sequencing system and method of use
WO2011028296A2 (en) * 2009-09-07 2011-03-10 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
US20130053252A1 (en) * 2009-09-25 2013-02-28 President & Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
KR101107315B1 (ko) * 2009-09-29 2012-01-20 한국과학기술연구원 ３′―하이드록실기에 형광을 띄는 장애그룹이 부착된 뉴클레오시드 삼인산을 가역적 종결자로서 이용한 ｄｎａ 염기서열 분석 방법
WO2011050938A1 (de) 2009-10-26 2011-05-05 Genovoxx Gmbh Konjugate von nukleotiden und methoden zu deren anwendung
US9056289B2 (en) 2009-10-27 2015-06-16 President And Fellows Of Harvard College Droplet creation techniques
WO2011056215A1 (en) 2009-11-03 2011-05-12 Landers James P Versatile, visible method for detecting polymeric analytes
WO2011057197A2 (en) 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8500979B2 (en) * 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
EP2526427A4 (de) * 2010-01-19 2013-07-24 Harvard College Vorrichtung und verfahren für schnelle diagnose von krankheitserregern
US9353342B2 (en) 2010-01-21 2016-05-31 Emd Millipore Corporation Cell culture and gradient migration assay methods and devices
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
EP2529238A4 (de) 2010-01-28 2014-10-08 The Regents Of The University Of Michigan Hängende tropfvorrichtungen, -systeme und/oder -verfahren
CA2786569C (en) 2010-01-29 2019-04-09 Micronics, Inc. Sample-to-answer microfluidic cartridge
US8518643B2 (en) * 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
WO2011106693A2 (en) * 2010-02-26 2011-09-01 The Regents Of The University Of Michigan Microscale western blot
WO2011108540A1 (ja) 2010-03-03 2011-09-09 国立大学法人大阪大学 ヌクレオチドを識別する方法および装置、ならびにポリヌクレオチドのヌクレオチド配列を決定する方法および装置
CN101817495B (zh) * 2010-03-25 2012-03-14 湖南大学 微流控芯片及其制备方法和应用
AU2011236503B2 (en) 2010-04-07 2014-10-30 Biosensia Patents Limited Flow control device for assays
EP2388337B1 (de) * 2010-04-30 2014-07-02 Nxp B.V. Messgerät und Herstellungsverfahren dafür
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
WO2011150226A1 (en) 2010-05-26 2011-12-01 Landers James P Method for detecting nucleic acids based on aggregate formation
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US8318094B1 (en) 2010-06-18 2012-11-27 Pacific Biosciences Of California, Inc. Substrate analysis systems
US8351053B2 (en) * 2010-06-25 2013-01-08 The Board Of Trustees Of The University Of Illinois Apparatus and method for in situ testing of microscale and nanoscale samples
EP2598804B1 (de) 2010-06-30 2021-10-20 Life Technologies Corporation Verfahren zur erzeugung eines ausgangssignals in einem isfet-array
TWI569025B (zh) 2010-06-30 2017-02-01 生命技術公司 用於測試離子感測場效電晶體（ｉｓｆｅｔ）陣列之裝置及方法
WO2012003363A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
US8653567B2 (en) 2010-07-03 2014-02-18 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
JP2012018039A (ja) * 2010-07-07 2012-01-26 Sony Corp マイクロ流路と核酸ハイブリダイゼーションのためのマイクロチップとカラム及び装置並びに方法
US8834847B2 (en) 2010-08-12 2014-09-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
US8715932B2 (en) 2010-08-20 2014-05-06 Intel Corporation Nucleic acid sequencing
EP2606242A4 (de) 2010-08-20 2016-07-20 Integenx Inc Mikrofluidikvorrichtungen mit mechanisch abgedichteten membranventilen
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US8465922B2 (en) 2010-08-26 2013-06-18 Pacific Biosciences Of California, Inc. Methods and systems for monitoring reactions
US8846331B2 (en) 2010-08-27 2014-09-30 The Regents Of The University Of Michigan Asynchronous magnetic bead rotation sensing systems and methods
US8444835B2 (en) 2010-09-09 2013-05-21 Intel Corporation Electronic and fluidic interface
US9618475B2 (en) 2010-09-15 2017-04-11 Life Technologies Corporation Methods and apparatus for measuring analytes
EP2619564B1 (de) 2010-09-24 2016-03-16 Life Technologies Corporation Transistorschaltungen mit zusammengehörigen paaren
JP6114694B2 (ja) 2010-10-04 2017-04-12 ジナプシス インコーポレイテッド 自動化された再使用可能な並行生物反応のための系および方法
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US8961764B2 (en) 2010-10-15 2015-02-24 Lockheed Martin Corporation Micro fluidic optic design
WO2012071437A1 (en) * 2010-11-23 2012-05-31 Biomerieux, Inc. Improved sample test cards
JP2012127696A (ja) * 2010-12-13 2012-07-05 Sharp Corp 分析装置および分析方法
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
WO2012138973A2 (en) 2011-04-06 2012-10-11 The University Of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
US9816993B2 (en) 2011-04-11 2017-11-14 The Regents Of The University Of Michigan Magnetically induced microspinning for super-detection and super-characterization of biomarkers and live cells
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
WO2012146377A1 (de) 2011-04-27 2012-11-01 Dmitry Cherkasov Methoden und komponenten zur detektion von nukleinsäureketten
WO2012151268A1 (en) 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system for high throughput optical and label free detection of analytes
WO2012151289A2 (en) 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system to detect aggregate formation on a substrate
WO2012150035A1 (de) 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-triphosphat-konjugate und methoden zu deren anwendung
US9188615B2 (en) 2011-05-09 2015-11-17 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
JP2014517291A (ja) 2011-05-18 2014-07-17 スリーエム イノベイティブ プロパティズ カンパニー サンプル処理装置のバルブ動作のためのシステム及び方法
WO2012158990A1 (en) 2011-05-18 2012-11-22 3M Innovative Properties Company Systems and methods for volumetric metering on a sample processing device
JP6235462B2 (ja) 2011-05-18 2017-11-22 スリーエム イノベイティブ プロパティズ カンパニー 試料処理装置内で材料の選択された体積の存在を検出するためのシステム及び方法
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
WO2012166742A2 (en) * 2011-05-27 2012-12-06 Genapsys, Inc. Systems and methods for genetic and biological analysis
DE202011108189U1 (de) 2011-06-08 2011-12-13 Albert-Ludwigs-Universität Freiburg Vorrichtung und Fluidikmodul zum Erzeugen einer Verdünnungsreihe
CA2840949A1 (en) 2011-07-06 2013-01-10 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
EP2748604A4 (de) * 2011-09-23 2015-05-13 Siemens Healthcare Diagnostics Mikrofluidische vorrichtung zum trennen von zellen aus einer flüssigkeit
CA2849917C (en) 2011-09-30 2020-03-31 Becton, Dickinson And Company Unitized reagent strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP3604555A1 (de) 2011-10-14 2020-02-05 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
TWI489110B (zh) * 2011-11-02 2015-06-21 Wistron Corp 生物晶片
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
CN103917870B (zh) * 2011-11-16 2016-04-13 贝克顿·迪金森公司 用于检测样品中的分析物的方法和系统
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
JP6232383B2 (ja) 2011-12-03 2017-11-15 イー・エム・デイー・ミリポア・コーポレイシヨン マイクロ流体の細胞培養のためのマイクロインキュベーションシステムおよび方法
KR101911436B1 (ko) * 2011-12-13 2018-10-25 삼성전자주식회사 높은 종횡비를 갖는 세포 포획용 필터
CN103160430A (zh) * 2011-12-13 2013-06-19 三星电子株式会社 具有高的长宽比的细胞捕获过滤器
CA2859761C (en) 2011-12-22 2023-06-20 President And Fellows Of Harvard College Compositions and methods for analyte detection
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
CN104053498B (zh) * 2012-01-13 2017-05-03 皇家飞利浦有限公司 使用在线栅上再循环的试剂的dna测序
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
RU2658773C2 (ru) 2012-02-03 2018-06-22 Бектон, Дикинсон Энд Компани Система и способ выполнения автоматизированных тестов над множеством биологических проб
ES2632423T3 (es) * 2012-02-03 2017-09-13 Pyrobett Pte Ltd Plataforma giratoria para realizar secuenciación de ácido nucleico
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
US9175348B2 (en) 2012-04-24 2015-11-03 Pacific Biosciences Of California, Inc. Identification of 5-methyl-C in nucleic acid templates
US9797817B2 (en) 2012-05-03 2017-10-24 The Regents Of The University Of Michigan Multi-mode separation for target detection
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9994805B2 (en) 2012-05-31 2018-06-12 The University Of North Carolina At Chapel Hill Dissolution guided wetting of structured surfaces
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
CA2877950C (en) 2012-06-27 2021-06-22 Advanced Liquid Logic Inc. Techniques and droplet actuator designs for reducing bubble formation
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN113528634A (zh) 2012-08-14 2021-10-22 10X基因组学有限公司 微胶囊组合物及方法
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2882001A1 (en) 2012-08-17 2014-02-20 Osaka University Sample analysis method
CN104884605B (zh) 2012-08-24 2018-05-18 耶鲁大学 用于高通量多重检测的系统、装置和方法
DE102012109026A1 (de) * 2012-09-25 2014-03-27 Eads Deutschland Gmbh Detektionsvorrichtung und Detektionsverfahren zur automatischen Bestimmung von Biomasse
WO2014047737A1 (en) * 2012-09-27 2014-04-03 Nanospeed Diagnostics Inc. Microfluidic chip for multi-analyte detection
GB201217390D0 (en) 2012-09-28 2012-11-14 Agplus Diagnostics Ltd Test device and sample carrier
CN110579435B (zh) 2012-10-15 2023-09-26 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
US20150330974A1 (en) 2012-11-19 2015-11-19 Apton Biosystems, Inc. Digital Analysis of Molecular Analytes Using Single Molecule Detection
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014100725A1 (en) 2012-12-21 2014-06-26 Micronics, Inc. Portable fluorescence detection system and microassay cartridge
KR102102123B1 (ko) 2012-12-21 2020-04-20 퍼킨엘머 헬스 사이언시즈, 아이엔씨. 유체 공학 회로 및 관련 제조 방법
CN107824233B (zh) 2012-12-21 2020-06-30 珀金埃尔默健康科学有限公司 用于微流体用途的低弹性膜
JP6282036B2 (ja) 2012-12-27 2018-02-21 クオンタムバイオシステムズ株式会社 物質の移動速度の制御方法および制御装置
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
AU2014205215B2 (en) 2013-01-11 2019-02-14 Becton, Dickinson And Company Low-cost point-of-care assay device
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US20140222837A1 (en) * 2013-02-01 2014-08-07 Frederick G. Strathmann Automated quality tool for monitoring of samples in a high-throughput assay
US9650406B2 (en) * 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
EP2970366B1 (de) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Nukleotidanaloga zur sequenzierung
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
JP6581074B2 (ja) 2013-03-15 2019-09-25 ライフ テクノロジーズ コーポレーション 一貫性のあるセンサ表面積を有する化学センサ
WO2014149780A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
WO2014149779A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical device with thin conductive element
WO2014152625A1 (en) 2013-03-15 2014-09-25 Genapsys, Inc. Systems and methods for biological analysis
WO2014139596A1 (en) 2013-03-15 2014-09-18 Illumina Cambridge Limited Modified nucleosides or nucleotides
CN104096526B (zh) * 2013-04-03 2016-03-23 国家纳米科学中心 一种全氟聚合物微反应器及其应用
CA2911303C (en) 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
CN105189784B (zh) 2013-05-07 2020-06-30 珀金埃尔默健康科学有限公司 用于制备和分析核酸的装置
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US20160091489A1 (en) * 2013-06-03 2016-03-31 University Of Florida Research Foundation, Incorporated Devices and methods for isolating cells
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
KR102291045B1 (ko) 2013-08-05 2021-08-19 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
WO2015027112A1 (en) 2013-08-22 2015-02-26 Apton Biosystems, Inc. Digital analysis of molecular analytes using electrical methods
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015042200A1 (en) 2013-09-18 2015-03-26 Osaka University Biomolecule sequencing devices, systems and methods
US9604214B2 (en) * 2013-10-01 2017-03-28 Owl biomedical, Inc. Cell sorting system using microfabricated components
US9803192B2 (en) 2013-10-04 2017-10-31 Cornell University Programmable and reconfigurable microcolumn affinity chromatography device, system, and methods of use thereof
JP2015077652A (ja) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 ナノギャップ電極およびその製造方法
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
US9983110B2 (en) 2013-11-04 2018-05-29 The Regents Of The University Of Michigan Asynchronous magnetic bead rotation (AMBR) microviscometer for analysis of analytes
JP6632525B2 (ja) 2013-11-06 2020-01-22 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ マイクロ流体デバイスならびにその製造方法および使用方法
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
BR112016010721B1 (pt) 2013-11-13 2021-06-01 Becton, Dickinson And Company Método e sistema de análise de uma amostra para um analito
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
WO2015077441A2 (en) 2013-11-20 2015-05-28 University Of Florida Research Foundation, Incorporated Antibody and aptamer ensemble for cell isolation and enrichment
WO2015079446A1 (en) * 2013-11-29 2015-06-04 Technion Research & Development Foundation Limited Metod and device for accelerated surface-based reactions
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP4086618A1 (de) * 2014-01-15 2022-11-09 Caliper Life Sciences, Inc. System für ein mikrofluidisches immunoassay unter verwendung magnetischer kügelchen
MX2016013156A (es) 2014-04-10 2017-02-14 10X Genomics Inc Dispositivos, sistemas y metodos fluidicos, para encapsular y dividir reactivos, y aplicaciones de los mismos.
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
EP3132060B1 (de) 2014-04-18 2019-03-13 Genapsys Inc. Verfahren und systeme zur nukleinsäureamplifikation
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
SG11201610691QA (en) 2014-06-26 2017-01-27 10X Genomics Inc Processes and systems for nucleic acid sequence assembly
CN113249435A (zh) 2014-06-26 2021-08-13 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
GB201414451D0 (en) * 2014-08-14 2014-10-01 Oxford Gene Technology Operations Ltd Hybridisation column for nucleic acid enrichment
US10544455B2 (en) 2014-10-03 2020-01-28 Life Technologies Corporation Sequencing methods, compositions and systems using terminator nucleotides
US10487357B2 (en) * 2014-10-03 2019-11-26 Life Technologies Corporation Methods of nucleic acid analysis using terminator nucleotides
AU2015334044B2 (en) 2014-10-14 2018-02-15 Becton, Dickinson And Company Blood sample management using open cell foam
PL3094252T3 (pl) 2014-10-14 2022-02-21 Becton, Dickinson And Company Zarządzanie próbką krwi z zastosowaniem pianki otwartokomórkowej
US10690627B2 (en) 2014-10-22 2020-06-23 IntegenX, Inc. Systems and methods for sample preparation, processing and analysis
WO2016062788A1 (en) 2014-10-24 2016-04-28 Ait Austrian Institute Of Technology Gmbh Microfluidic chip for biological analysis
AU2015339148B2 (en) 2014-10-29 2022-03-10 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107427793B (zh) * 2014-11-07 2021-05-28 溶液充氧公司 用于将气体溶解到液体中的设备
CN105624020B (zh) * 2014-11-07 2017-11-03 深圳华大基因研究院 用于检测dna片段的碱基序列的微流控芯片
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
KR102593647B1 (ko) 2014-12-18 2023-10-26 라이프 테크놀로지스 코포레이션 트랜스미터 구성을 갖춘 높은 데이터율 집적 회로
EP3234575B1 (de) 2014-12-18 2023-01-25 Life Technologies Corporation Vorrichtung zur messung von analyten unter verwendung von grossflächigen fet-arrays
KR102321863B1 (ko) 2015-01-12 2021-11-08 10엑스 제노믹스, 인크. 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리
KR20170106979A (ko) 2015-01-13 2017-09-22 10엑스 제노믹스, 인크. 구조 변이 및 위상 조정 정보를 시각화하기 위한 시스템 및 방법
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
SG11201705996PA (en) 2015-02-09 2017-09-28 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
EP4286516A3 (de) 2015-02-24 2024-03-06 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
EP3936619A1 (de) 2015-02-24 2022-01-12 10X Genomics, Inc. Verfahren zur sequenzierung von nukleinsäuren in gesteuertem ausmass
CN110075939B (zh) 2015-03-10 2022-06-07 贝克顿·迪金森公司 生物流体微样本管理装置
US10598625B2 (en) 2015-04-08 2020-03-24 Board of Regents, The University System of Texas Methods and systems for the detection of analytes
US10590461B2 (en) 2015-04-21 2020-03-17 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
US10677793B2 (en) 2015-04-21 2020-06-09 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US9868947B2 (en) 2015-05-04 2018-01-16 Washington University Compositions and methods for the construction of a random allelic series
CN106117288B (zh) 2015-05-08 2019-10-15 生捷科技控股公司 二硫键连接的可逆终止子
CN108026494A (zh) 2015-06-05 2018-05-11 米罗库鲁斯公司 限制蒸发和表面结垢的空气基质数字微流控装置和方法
WO2016197106A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Evaporation management in digital microfluidic devices
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
US10150994B2 (en) * 2015-07-22 2018-12-11 Qiagen Waltham, Inc. Modular flow cells and methods of sequencing
CN108136395B (zh) 2015-09-01 2022-05-13 贝克顿·迪金森公司 用于分离样本相的深度过滤装置
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
CN113604546A (zh) 2015-09-22 2021-11-05 特韦斯特生物科学公司 用于核酸合成的柔性基底
US10577643B2 (en) * 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
EP3362032A4 (de) 2015-10-13 2019-05-01 President and Fellows of Harvard College Systeme und verfahren zur herstellung und verwendung von gel-mikrokugeln
FR3042796B1 (fr) * 2015-10-21 2022-04-29 Centre Nat Rech Scient Dispositif microfluidique pour confiner un echantillon
MX2018005611A (es) 2015-11-03 2018-11-09 Harvard College Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico.
WO2017087894A1 (en) 2015-11-19 2017-05-26 Pacific Bioscienes Of California, Inc. Compounds and systems for improving signal detection
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
WO2017095813A1 (en) 2015-11-30 2017-06-08 Intabio, Inc. Devices and methods for sample characterization
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
EP3882357B1 (de) 2015-12-04 2022-08-10 10X Genomics, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
CN108779491B (zh) 2016-02-11 2021-03-09 10X基因组学有限公司 用于全基因组序列数据的从头组装的系统、方法和介质
US10988501B2 (en) * 2016-04-22 2021-04-27 Mgi Tech Co., Ltd. Reversibly blocked nucleoside analogues and their use
CA3210120C (en) 2016-04-25 2024-04-09 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
WO2017197338A1 (en) * 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3478702A4 (de) 2016-06-27 2020-03-18 Dana-Farber Cancer Institute, Inc. Verfahren zur messung der rna-translationsraten
WO2018005390A1 (en) * 2016-06-30 2018-01-04 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
AU2017289055A1 (en) * 2016-06-30 2018-12-20 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
USD812766S1 (en) * 2016-07-12 2018-03-13 EMULATE, Inc. Microfluidic chip for use with a fluid perfusion module
CN106076445B (zh) * 2016-07-18 2018-06-15 天津德祥生物技术有限公司 微流控试剂卡及其检测方法和应用
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光ｉｎ ｓｉｔｕ配列決定による検出のための核酸配列ライブラリの作製法
CN109923216A (zh) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
USD842493S1 (en) * 2016-09-07 2019-03-05 EMULATE, Inc. Microfluidic chip without pressure features for use with a fluid perfusion module
USD816861S1 (en) * 2016-09-07 2018-05-01 EMULATE, Inc. Transparent microfluidic chip without pressure features for use with a fluid perfusion module
KR102217487B1 (ko) 2016-09-21 2021-02-23 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
US9770717B1 (en) * 2016-11-03 2017-09-26 International Business Machines Corporation Microfluidic chip with bead integration system
JP7348066B2 (ja) 2016-11-11 2023-09-20 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法，以及其制造方法
EA201991262A1 (ru) 2016-12-16 2020-04-07 Твист Байосайенс Корпорейшн Библиотеки вариантов иммунологического синапса и их синтез
WO2018118992A1 (en) * 2016-12-19 2018-06-28 Quantum-Si Incorporated Loading molecules into sample wells for analysis
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108239661B (zh) * 2016-12-23 2020-06-12 中国科学院深圳先进技术研究院 微流控装置、将物质导入细胞的系统及方法
WO2018126082A1 (en) 2016-12-28 2018-07-05 Miroculis Inc. Digital microfluidic devices and methods
EP4310183A3 (de) 2017-01-30 2024-02-21 10X Genomics, Inc. Verfahren und systeme zur tröpfchenbasierten einzelzellenbarcodierung
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018148700A1 (en) 2017-02-13 2018-08-16 Yale University High-throughput single-cell polyomics
US11474103B2 (en) 2017-02-17 2022-10-18 Koniku Inc. Systems for detection
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
JP7305611B2 (ja) * 2017-03-17 2023-07-10 アプトン バイオシステムズ インコーポレイテッド 配列決定および高分解能画像化の方法
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
EP3625715A4 (de) 2017-05-19 2021-03-17 10X Genomics, Inc. Systeme und verfahren zur analyse von datensätzen
EP4230746A3 (de) 2017-05-26 2023-11-01 10X Genomics, Inc. Einzelzellanalyse von transposase-zugänglichem chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
KR20240013290A (ko) 2017-06-12 2024-01-30 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
CN107515210B (zh) * 2017-06-12 2020-04-14 南京大学 一种手持式高通量矩阵电致化学发光芯片分析系统
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3645546A4 (de) 2017-06-30 2021-12-01 Solstice Biologics, Ltd. Chirale phosphoramidithilfsmittel und verfahren zu ihrer verwendung
CN110892258A (zh) 2017-07-24 2020-03-17 米罗库鲁斯公司 具有集成的血浆收集设备的数字微流控系统和方法
US11280706B2 (en) * 2017-08-01 2022-03-22 Essenlix Corporation Dilution calibration
CN111587149B (zh) 2017-09-01 2022-11-11 米罗库鲁斯公司 数字微流控设备及其使用方法
WO2019049944A1 (en) * 2017-09-07 2019-03-14 Sony Corporation PARTICLE CAPTURE CHAMBER, PARTICLE CAPTURE CHIP, PARTICLE CAPTURE METHOD, APPARATUS, AND PARTICLE ANALYSIS SYSTEM
GB2581620A (en) 2017-09-11 2020-08-26 Twist Bioscience Corp GPCR binding proteins and synthesis thereof
CN111566224A (zh) 2017-09-21 2020-08-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP4241882A3 (de) 2017-10-27 2023-12-06 10X Genomics, Inc. Verfahren zur probenvorbereitung und -analyse
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3728555A1 (de) * 2017-12-21 2020-10-28 Politecnico di Milano Erythrozyten zur arzneimittelabgabe
EP3735459A4 (de) 2018-01-04 2021-10-06 Twist Bioscience Corporation Digitale informationsspeicherung auf dna-basis
WO2019148198A1 (en) 2018-01-29 2019-08-01 Intabio, Inc. Devices, methods and kits for sample characterization
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11041834B2 (en) * 2018-03-06 2021-06-22 Waters Technologies Corporation High surface area chromatographic device with low pressure drop
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019204510A1 (en) * 2018-04-17 2019-10-24 Reolab Programmable microchannel systems for analyte detection
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CA3101624A1 (en) 2018-05-31 2019-12-05 Intabio, Inc. Software for microfluidic systems interfacing with mass spectrometry
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
EP3775834A4 (de) 2018-07-09 2021-03-17 Hewlett-Packard Development Company, L.P. Analytfangvorrichtungen mit fluidischen ausstossvorrichtungen
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
KR20210047364A (ko) * 2018-09-19 2021-04-29 앱톤 바이오시스템즈, 인코포레이티드 밀집 패킹된 분석물층 및 검출 방법
EP3856753A4 (de) 2018-09-28 2022-11-16 Centrillion Technology Holdings Corporation Disulfidvernetzte reversible terminatoren
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN109939752A (zh) * 2018-12-11 2019-06-28 安博特纳米生物科技有限公司 微流道结构及生物检测平台
AU2019411266A1 (en) * 2018-12-17 2021-01-07 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
US11782023B2 (en) * 2018-12-19 2023-10-10 Regeneron Pharmaceuticals, Inc. Ce-western applications for antibody development
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
TW202043486A (zh) 2019-01-29 2020-12-01 美商伊路米納有限公司 定序套組
TW202045709A (zh) 2019-01-29 2020-12-16 美商伊路米納有限公司 流體槽
EP3921418A4 (de) * 2019-02-06 2023-02-08 Singular Genomics Systems, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3924505A1 (de) 2019-02-12 2021-12-22 10X Genomics, Inc. Verfahren zur verarbeitung von nukleinsäuremolekülen
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
EP3930753A4 (de) 2019-02-26 2023-03-29 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für glp1-rezeptor
EP3938537A1 (de) 2019-03-11 2022-01-19 10X Genomics, Inc. Systeme und verfahren zur verarbeitung von optisch markierten perlen
CN111744562A (zh) * 2019-03-28 2020-10-09 天津大学 一种用于检测微污染物的微流控芯片
WO2020210292A1 (en) 2019-04-08 2020-10-15 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
CN114729342A (zh) 2019-06-21 2022-07-08 特韦斯特生物科学公司 基于条形码的核酸序列装配
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
CN115003415A (zh) * 2020-01-22 2022-09-02 豪夫迈·罗氏有限公司 用于下一代测序库制备的微流控珠粒捕获装置和方法
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
WO2022115513A1 (en) * 2020-11-25 2022-06-02 University Of Maryland, College Park Heterogeneous microparticles, and systems and methods of making and use thereof
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2023278704A1 (en) 2021-06-30 2023-01-05 Dana-Farber Cancer Institute, Inc. Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking
FR3126890B1 (fr) * 2021-09-13 2024-03-29 Commissariat Energie Atomique Composant microfluidique employé pour une mesure d'impédance électrique à travers un objet biologique
WO2023059702A1 (en) * 2021-10-05 2023-04-13 Mayo Foundation For Medical Education And Research Cell isolation and reagent exchange in a microfluidic device
US11857961B2 (en) 2022-01-12 2024-01-02 Miroculus Inc. Sequencing by synthesis using mechanical compression

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4390403A (en) 1981-07-24 1983-06-28 Batchelder J Samuel Method and apparatus for dielectrophoretic manipulation of chemical species
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
SE458968B (sv) * 1987-06-16 1989-05-22 Wallac Oy Biospecifikt analysfoerfarande foer flera analyter i vilket ingaar partikelraekning och maerkning med fluorescerande maerksubstanser
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
EP0376611A3 (de) 1988-12-30 1992-07-22 The Board Of Trustees Of The Leland Stanford Junior University Elektroforetische Vorrichtung
US5122614A (en) 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5770029A (en) 1996-07-30 1998-06-23 Soane Biosciences Integrated electrophoretic microdevices
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5498392A (en) 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO1994013623A1 (en) * 1992-12-11 1994-06-23 Chiron Corporation Synthesis of encoded polymers
FR2703052B1 (fr) 1993-03-26 1995-06-02 Pasteur Institut Nouvelle méthode de séquençage d'acides nucléiques.
EP0620432B1 (de) 1993-04-15 2004-08-25 Zeptosens AG Verfahren zur Steuerung der Probeneinführung bei Mikrosäulentrennungstechniken und Probenentnahmevorrichtungen
DE4316320A1 (de) * 1993-05-15 1994-11-17 Degussa Verfahren zur Herstellung von Natriumperborat-monohydrat
EP1347493A3 (de) 1993-05-28 2005-11-23 Baylor College Of Medicine Verfahren und Gerät zur Desorption und Ionisierung von Analyte
US5525711A (en) 1994-05-18 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs as fluorescent DNA probes
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5650234A (en) 1994-09-09 1997-07-22 Surface Engineering Technologies, Division Of Innerdyne, Inc. Electrophilic polyethylene oxides for the modification of polysaccharides, polypeptides (proteins) and surfaces
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5571410A (en) 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US5603351A (en) 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
JP3309644B2 (ja) * 1995-05-26 2002-07-29 ソニー株式会社 データカートリッジドライブ
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5747349A (en) 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6399023B1 (en) 1996-04-16 2002-06-04 Caliper Technologies Corp. Analytical system and method
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
GB2313147B (en) 1996-05-14 2000-08-23 Europ Component Co Ltd Seat belt buckle
BR9710054A (pt) * 1996-06-28 2000-01-11 Caliper Techn Corp Aparelhos para separar compostos de teste para um efeito sobre um sistema bioquìmico e para detectar ummefeito de um composto de teste sobre um sistema bioquìmico, processos de determinação de se uma amostra contém um composto capaz de afetar um sistema bioquìmico, de separação de uma pluralidade de compostos de teste para um efeito sobre um sistema bioquìmico e usos de um sistema microfluido e de um substrato de ensaio.
US5779868A (en) 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
AU726987B2 (en) 1996-06-28 2000-11-30 Caliper Life Sciences, Inc. Electropipettor and compensation means for electrophoretic bias
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
US5699157A (en) 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US6447727B1 (en) 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
WO1998033939A1 (fr) * 1997-01-31 1998-08-06 Hitachi, Ltd. Procede pour determiner une sequence de base d'acide nucleique et appareil correspondant
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
AU746892B2 (en) * 1997-04-04 2002-05-02 Caliper Life Sciences, Inc. Closed-loop biochemical analyzers
US6235471B1 (en) * 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US5976336A (en) 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
KR100351531B1 (ko) 1997-04-25 2002-09-11 캘리퍼 테크놀로지스 코포레이션 기하형상이 개선된 채널을 채용하는 미소 유체 장치
US6090251A (en) 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
US6613512B1 (en) 1997-06-09 2003-09-02 Caliper Technologies Corp. Apparatus and method for correcting for variable velocity in microfluidic systems
US5869004A (en) 1997-06-09 1999-02-09 Caliper Technologies Corp. Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems
US5882465A (en) 1997-06-18 1999-03-16 Caliper Technologies Corp. Method of manufacturing microfluidic devices
US5959291A (en) 1997-06-27 1999-09-28 Caliper Technologies Corporation Method and apparatus for measuring low power signals
US6001231A (en) 1997-07-15 1999-12-14 Caliper Technologies Corp. Methods and systems for monitoring and controlling fluid flow rates in microfluidic systems
US6042721A (en) * 1997-07-23 2000-03-28 Fabco Industries, Inc. Effluent treatment apparatus
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
JP2001515204A (ja) 1997-09-02 2001-09-18 カリパー テクノロジーズ コーポレイション 電気流体制御および電気熱制御を有するマイクロ流体システム
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6012902A (en) 1997-09-25 2000-01-11 Caliper Technologies Corp. Micropump
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
US5948227A (en) 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
EP1044370B1 (de) 1997-12-30 2017-08-23 Caliper Life Sciences, Inc. Software zur anzeige chromatographischer trenndaten
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6100541A (en) 1998-02-24 2000-08-08 Caliper Technologies Corporation Microfluidic devices and systems incorporating integrated optical elements
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
EP1046032A4 (de) * 1998-05-18 2002-05-29 Univ Washington Patrone zur flüssigkeitsanalyse
US6306590B1 (en) * 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
AU5479599A (en) 1998-08-11 2000-03-06 Caliper Technologies Corporation Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
EP1157270A1 (de) * 1999-02-03 2001-11-28 Aclara BioSciences, Inc. Steuerung eines mehrkanal-mikrofluidiksystems
DE60044490D1 (de) * 1999-02-23 2010-07-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
US20010035351A1 (en) 2000-03-10 2001-11-01 Simpson Peter C. Cross channel device for serial sample injection

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EP2177627A1 (de) 2010-04-21
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WO2000050172A1 (en) 2000-08-31
ATE556149T1 (de) 2012-05-15
EP1163052A4 (de) 2002-08-14
US20090137413A1 (en) 2009-05-28
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US20160040226A1 (en) 2016-02-11
US20030215862A1 (en) 2003-11-20
EP1163052B1 (de) 2010-06-02
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US6632655B1 (en) 2003-10-14
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US7344865B2 (en) 2008-03-18
US20040096960A1 (en) 2004-05-20
US7105300B2 (en) 2006-09-12
US6613513B1 (en) 2003-09-02
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US9101928B2 (en) 2015-08-11
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US20170356040A1 (en) 2017-12-14
ATE469699T1 (de) 2010-06-15
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