DE60042775D1 - Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen - Google Patents

Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen

Info

Publication number
DE60042775D1
DE60042775D1 DE60042775T DE60042775T DE60042775D1 DE 60042775 D1 DE60042775 D1 DE 60042775D1 DE 60042775 T DE60042775 T DE 60042775T DE 60042775 T DE60042775 T DE 60042775T DE 60042775 D1 DE60042775 D1 DE 60042775D1
Authority
DE
Germany
Prior art keywords
hybridization
pools
probes
improved sequencing
sbh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60042775T
Other languages
English (en)
Inventor
Radoje Drmanac
Snezana Drmanac
David Kita
Cory Cooke
Chongjun Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Callida Genomics Inc
Original Assignee
Callida Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Callida Genomics Inc filed Critical Callida Genomics Inc
Application granted granted Critical
Publication of DE60042775D1 publication Critical patent/DE60042775D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
DE60042775T 1999-01-06 2000-01-06 Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen Expired - Lifetime DE60042775D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11528499P 1999-01-06 1999-01-06
PCT/US2000/000458 WO2000040758A2 (en) 1999-01-06 2000-01-06 Enhanced sequencing by hybridization using pools of probes

Publications (1)

Publication Number Publication Date
DE60042775D1 true DE60042775D1 (de) 2009-10-01

Family

ID=22360367

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60042775T Expired - Lifetime DE60042775D1 (de) 1999-01-06 2000-01-06 Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen

Country Status (6)

Country Link
US (4) US6864052B1 (de)
EP (2) EP2145963A1 (de)
AT (1) ATE440148T1 (de)
AU (1) AU2496900A (de)
DE (1) DE60042775D1 (de)
WO (1) WO2000040758A2 (de)

Families Citing this family (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
DE60042775D1 (de) * 1999-01-06 2009-10-01 Callida Genomics Inc Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
AU5497100A (en) * 1999-06-19 2001-01-09 Hyseq, Inc. Improved methods of sequence assembly in sequencing by hybridization
US7504213B2 (en) 1999-07-09 2009-03-17 Agilent Technologies, Inc. Methods and apparatus for preparing arrays comprising features having degenerate biopolymers
EP1322780A4 (de) * 2000-07-27 2005-08-03 Univ Australian Kombinatorische sonden und deren verwendung
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
EP1370690B1 (de) * 2001-03-16 2012-03-14 Kalim Mir Arrays und verfahren zur deren verwendung
WO2002090599A1 (en) * 2001-05-09 2002-11-14 Genetic Id, Inc. Universal microarray system
US20040009484A1 (en) * 2002-07-11 2004-01-15 Wolber Paul K. Methods for evaluating oligonucleotide probes of variable length
WO2004029586A1 (en) * 2002-09-27 2004-04-08 Nimblegen Systems, Inc. Microarray with hydrophobic barriers
CN103397082B (zh) 2003-02-26 2017-05-31 考利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
US20060226140A1 (en) * 2005-01-25 2006-10-12 Mr. Sake, Llc Heated tray
US20060240443A1 (en) * 2005-04-20 2006-10-26 Combimatrix Corporation Microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method
JP5331476B2 (ja) 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP2546360A1 (de) 2005-10-07 2013-01-16 Callida Genomics, Inc. Selbstangeordnete einzelne Molekülarrays und Verwendungen davon
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
SG10201405158QA (en) * 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP1994180A4 (de) 2006-02-24 2009-11-25 Callida Genomics Inc Genomsequenzierung auf dna-arrays mit hohem durchsatz
US7910302B2 (en) * 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
US20090111705A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
EP2099935B1 (de) * 2006-11-30 2014-02-26 The Regents Of The University Of California Array für den nachweis von mikroben
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
EP4310194A2 (de) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8298768B2 (en) * 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
WO2009059022A1 (en) 2007-10-30 2009-05-07 Complete Genomics, Inc. Apparatus for high throughput sequencing of nucleic acids
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
DK2565279T3 (en) 2007-12-05 2015-02-16 Complete Genomics Inc Efficient base determination in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US8852864B2 (en) * 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
WO2010028288A2 (en) 2008-09-05 2010-03-11 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
EP2393944A4 (de) * 2009-02-03 2013-11-27 Complete Genomics Inc Indexierung einer referenzsequenz für oligomersequenz-mapping
US8731843B2 (en) * 2009-02-03 2014-05-20 Complete Genomics, Inc. Oligomer sequences mapping
US8615365B2 (en) * 2009-02-03 2013-12-24 Complete Genomics, Inc. Oligomer sequences mapping
EP2511843B1 (de) 2009-04-29 2016-12-21 Complete Genomics, Inc. Verfahren und System zum Aufrufen von Variationen in einer Polynukleotidprobensequenz in Bezug auf eine Referenzpolynukleotidsequenz
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
JP2012525147A (ja) 2009-04-30 2012-10-22 グッド スタート ジェネティクス， インコーポレイテッド 遺伝マーカーを評価するための方法および組成物
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
DK2977455T3 (da) 2009-06-15 2020-07-13 Complete Genomics Inc Fremgangsmåde til langfragmentaflæsnings-sekventering
WO2011050340A1 (en) 2009-10-23 2011-04-28 Life Technologies Corporation Systems and methods for error correction in dna sequencing
DK2556171T3 (en) 2010-04-05 2015-12-14 Prognosys Biosciences Inc Spatially CODED BIOLOGICAL ASSAYS
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US9880089B2 (en) 2010-08-31 2018-01-30 Complete Genomics, Inc. High-density devices with synchronous tracks for quad-cell based alignment correction
US9671344B2 (en) 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
EP3572528A1 (de) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direkte erfassung, amplifikation und sequenzierung einer ziel-dna unter verwendung immobilisierter primer
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
WO2012103031A2 (en) 2011-01-25 2012-08-02 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US20120219950A1 (en) 2011-02-28 2012-08-30 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
EP3150750B1 (de) 2011-04-08 2018-12-26 Prognosys Biosciences, Inc. Peptidkonstrukte und testsysteme dafür
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US9228233B2 (en) 2011-10-17 2016-01-05 Good Start Genetics, Inc. Analysis methods
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP2844771A4 (de) 2012-05-04 2015-12-02 Complete Genomics Inc Verfahren zur bestimmung der absoluten genomweiten kopienzahlveränderungen komplexer tumoren
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
EP3937179A1 (de) 2012-05-21 2022-01-12 Distributed Bio Inc Epitopfokussierung durch variable effektive antigenoberflächenkonzentration
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
CN105431553B (zh) * 2013-05-29 2020-02-07 生物辐射实验室股份有限公司 用于在基于乳液的微流体中测序的系统和方法
EP3005200A2 (de) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Verfahren und systeme zur speicherung von sequenzlesedaten
AU2014278730B2 (en) 2013-06-13 2020-12-10 F. Hoffmann-La Roche Ag Statistical analysis for non-invasive sex chromosome aneuploidy determination
CN111662960B (zh) 2013-06-25 2024-04-12 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
WO2015027245A1 (en) 2013-08-23 2015-02-26 Complete Genomics, Inc. Long fragment de novo assembly using short reads
WO2015042708A1 (en) 2013-09-25 2015-04-02 Bio-Id Diagnostic Inc. Methods for detecting nucleic acid fragments
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015057565A1 (en) 2013-10-18 2015-04-23 Good Start Genetics, Inc. Methods for assessing a genomic region of a subject
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
CA2960821A1 (en) 2014-09-09 2016-03-17 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
JP2017536087A (ja) 2014-09-24 2017-12-07 グッド スタート ジェネティクス， インコーポレイテッド 遺伝子アッセイのロバストネスを増大させるためのプロセス制御
CA3010579A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
DK3901281T3 (da) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4242325A3 (de) 2020-04-22 2023-10-04 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158054A1 (de) 2020-06-02 2023-04-05 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (de) 2020-06-10 2023-04-19 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
EP0723598B1 (de) 1993-09-27 2004-01-14 Arch Development Corporation Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
JPH09507121A (ja) * 1993-10-26 1997-07-22 アフィマックス テクノロジーズ ナームロゼ ベノートスハップ 生物学的チップ上の核酸プローブアレー
US20020042048A1 (en) 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
CA2300940A1 (en) 1997-08-15 1999-02-25 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
US6355419B1 (en) * 1998-04-27 2002-03-12 Hyseq, Inc. Preparation of pools of nucleic acids based on representation in a sample
DE60042775D1 (de) * 1999-01-06 2009-10-01 Callida Genomics Inc Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
WO2000056937A2 (en) * 1999-03-25 2000-09-28 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
US8787443B2 (en) 2010-10-05 2014-07-22 Microsoft Corporation Content adaptive deblocking during video encoding and decoding
US11196198B2 (en) 2019-05-03 2021-12-07 Foxconn (Kunshan) Computer Connector Co., Ltd. Card edge connector with improved contacts

Also Published As

Publication number Publication date
US20110039731A1 (en) 2011-02-17
US7582431B2 (en) 2009-09-01
EP2145963A1 (de) 2010-01-20
EP1144684B1 (de) 2009-08-19
US8034566B2 (en) 2011-10-11
AU2496900A (en) 2000-07-24
EP1144684A2 (de) 2001-10-17
US6864052B1 (en) 2005-03-08
WO2000040758A3 (en) 2000-11-02
WO2000040758A2 (en) 2000-07-13
ATE440148T1 (de) 2009-09-15
US20050191656A1 (en) 2005-09-01
US20090280481A1 (en) 2009-11-12

Similar Documents

Publication Publication Date Title
DE60042775D1 (de) Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
DE60137722D1 (de) Verwendung von mass-matched nukleotide in der analyse von oligonukleotidmischungen sowie in der hoch-multiplexen nukleinsäuresequenzierung
WO2003106620A3 (en) NUCLEIC ACID SEQUENCING BY SIGNAL STRETCHING AND DATA INTEGRATION
GEP20125384B (en) Representations of nucleotide sequences
ATE321883T1 (de) DE NOVO ODER ßUNIVERSALERß SEQUENZIERUNGS-ARRAY
DE602005009693D1 (de) 2"-nitrobenzyl-modifizierte ribonukleotide
MX9400837A (es) Metodos y aparatos para la determinacion de secuencia de adn.
DK0586618T3 (da) Anvendelse af nuklainsyreanaloger i diagnostiske og analytiske fremgangsmåder
ATE444377T1 (de) Nukleinsäurecharakterisierung
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
ATE244259T1 (de) Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
ATE412002T1 (de) Gruppen zum einfangen von nukleinsäuren
SG170028A1 (en) High throughput genome sequencing on dna arrays
IL173314A0 (en) Determining the sequence of a polynucleotide
DE3578786D1 (de) Als sonden zum nachweis von schaedlichen, in chromosom-dns enthaltenen genen zu verwendende deoxyribonukleinsaeuremolekuele.
ATE266738T1 (de) Genomische multi-loci analyse mittels eines verbesserten verfahrens basierend auf zyklischer dna-sequenzierung
EA200500911A1 (ru) Планшеты и системы для крупномасштабной электропорации и способы их применения
DE602005003369D1 (de) Neue sequenz um die expression einer nukleinsäure zu erhöhen
SG155774A1 (en) Method to generate or determine nucleic acid tags corresponding to the terminal ends of dna molecules using sequences analysis of gene expression (terminal sage)
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
WO2002016650A3 (en) Compositions and methods for exon profiling
DE69929365D1 (de) Systeme und verfahren zum sequenzieren durch hybridation
ATE267879T1 (de) Polymerasesignalversuch
ATE316154T1 (de) Nachweisverfahren der nukleinsäurehybridisierung

Legal Events

Date Code Title Description
8364 No opposition during term of opposition