DE60029323D1 - Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate - Google Patents

Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate

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Publication number
DE60029323D1
DE60029323D1 DE60029323T DE60029323T DE60029323D1 DE 60029323 D1 DE60029323 D1 DE 60029323D1 DE 60029323 T DE60029323 T DE 60029323T DE 60029323 T DE60029323 T DE 60029323T DE 60029323 D1 DE60029323 D1 DE 60029323D1
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Prior art keywords
nucleic acid
genomic dna
purchase
dna samples
high rate
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DE60029323T3 (de
DE60029323T2 (de
Inventor
W Laird
A Eads
D Danenberg
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University of Southern California USC
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University of Southern California USC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
DE60029323.8T 1999-05-14 2000-05-12 Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate Expired - Lifetime DE60029323T3 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/311,912 US6331393B1 (en) 1999-05-14 1999-05-14 Process for high-throughput DNA methylation analysis
US311912 1999-05-14
PCT/US2000/013029 WO2000070090A1 (en) 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis
EP00928969.5A EP1185695B2 (de) 1999-05-14 2000-05-12 Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate

Publications (3)

Publication Number Publication Date
DE60029323D1 true DE60029323D1 (de) 2006-08-24
DE60029323T2 DE60029323T2 (de) 2007-07-05
DE60029323T3 DE60029323T3 (de) 2017-03-30

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Family Applications (1)

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DE60029323.8T Expired - Lifetime DE60029323T3 (de) 1999-05-14 2000-05-12 Verfahren zur analyse der dna-methylierung mit hoher durchsatzrate

Country Status (11)

Country Link
US (4) US6331393B1 (de)
EP (2) EP1185695B2 (de)
JP (2) JP4044290B2 (de)
AT (1) ATE332980T2 (de)
AU (2) AU775798B2 (de)
CA (1) CA2372665C (de)
CY (1) CY1118000T1 (de)
DE (1) DE60029323T3 (de)
DK (2) DK1185695T4 (de)
ES (2) ES2267535T5 (de)
WO (1) WO2000070090A1 (de)

Families Citing this family (256)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
JP2004505612A (ja) * 2000-03-31 2004-02-26 ユニバーシティ・オブ・サザン・カリフォルニア 食道腺ガンに関する後成的配列
EP1274866A2 (de) * 2000-04-06 2003-01-15 Epigenomics AG Diagnose von krankheiten, welche mit metastasierung assoziiert sind.
DE60125065T2 (de) * 2000-08-25 2007-06-28 Lovelace Respiratory Research Institute, Albuquerque Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr
US20020058265A1 (en) * 2000-09-15 2002-05-16 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US6844152B1 (en) 2000-09-15 2005-01-18 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
GB0025913D0 (en) 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
JP2004512050A (ja) * 2000-10-23 2004-04-22 キャンサー・リサーチ・テクノロジー・リミテッド 核酸の増幅およびプロファイリングに関連する物質および方法
US20030188326A1 (en) 2000-11-03 2003-10-02 Dana Farber Cancer Institute Methods and compositions for the diagnosis of cancer susceptibilities and defective DNA repair mechanisms and treatment thereof
DE10056802B4 (de) * 2000-11-14 2005-06-16 Epigenomics Ag Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik
DE10104937B4 (de) * 2001-01-29 2005-03-17 Epigenomics Ag Fluoreszenzpolarisation 2
US6893820B1 (en) * 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
ATE325894T1 (de) * 2001-03-01 2006-06-15 Epigenomics Ag Verfahren und rechnerprogrammprodukte zur erfassung der biologischen wirkung und/oder aktivität von arzneimitteln, chemischen substanzen und/oder pharmazeuitschen verbindungen auf der basis ihrer effekte auf den methylierungszustand der dna
ATE429511T1 (de) * 2001-03-01 2009-05-15 Epigenomics Ag Verfahren zur entwicklung von gensätzen zu diagnostischen und therapeutischen zwecken auf grundlage des expressions- und methylierungsstatus der gene
DE10112515B4 (de) * 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US20020197639A1 (en) * 2001-06-08 2002-12-26 Shia Michael A. Methods and products for analyzing nucleic acids based on methylation status
DE10128508A1 (de) * 2001-06-14 2003-02-06 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren
DE10128509A1 (de) * 2001-06-14 2003-01-02 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata- und Nierenkarzinomen
DE10130800B4 (de) 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
DE10132211A1 (de) * 2001-06-27 2003-01-16 Epigenomics Ag Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)
WO2003012051A2 (en) 2001-07-31 2003-02-13 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Inhibitor of dna methylation
DE10139283A1 (de) * 2001-08-09 2003-03-13 Epigenomics Ag Verfahren und Nukleinsäuren zur Analyse von Colon-Krebs
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
JP4381142B2 (ja) * 2001-11-16 2009-12-09 ザ ジョーンズ ホプキンス ユニバーシティー スクール オブ メディシン 前立腺癌の検出法
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
AU2002360474A1 (en) * 2001-12-03 2003-06-17 Illumina, Inc. Multiplexed methylation detection methods
EP1470254A2 (de) * 2002-01-30 2004-10-27 Epigenomics AG Methode zur analyse von cytosin-methylierungsmustern
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US20060258856A1 (en) * 2002-02-13 2006-11-16 Medimolecular Pty. Ltd. Method of isolating nucleic acids from stool samples
KR100470221B1 (ko) * 2002-02-20 2005-02-05 굿젠 주식회사 유전자 프로모터 ＣｐＧ 아일랜드의 메틸화 여부를분석하는 염기서열분석형 올리고뉴클레오타이드칩, 이의제조방법 및 이를 이용한 암 검색방법
EP1340818A1 (de) * 2002-02-27 2003-09-03 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von Kolonkrebszellen
ES2253461T3 (es) * 2002-03-05 2006-06-01 Epigenomics Ag Procedimiento y dispositivo para la determinacion de la especificidad del tejido y del adn que flota libre en tejidos corporales.
WO2003085132A2 (en) * 2002-04-09 2003-10-16 Epigenomics Ag Method for analysis of methylated nucleic acids
US7124516B2 (en) * 2002-06-21 2006-10-24 Yoshinori Shiraishi Device for removing water drops from an umbrella and foundation promotion system of the device
JP4384603B2 (ja) * 2002-06-26 2009-12-16 コールド スプリング ハーバー ラボラトリー メチル化プロファイルを測定するための方法および組成物
WO2004013284A2 (en) * 2002-08-02 2004-02-12 Tufts University A method to assess genomic dna methylation using high-performance liquid chromatography-electospray ionization mass spectrometry
DE10236406C1 (de) * 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
AU2003236461B2 (en) 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
JP2006500034A (ja) * 2002-09-19 2006-01-05 アプレラ コーポレイション 標的を検出するための方法および組成物
CA2500255A1 (en) * 2002-10-01 2004-04-29 Epigenomics Ag Method and nucleic acids for the treatment of breast cell proliferative disorders
US7238518B2 (en) 2002-10-04 2007-07-03 Nisshinbo Industries, Inc. Oligonucleotide-immobilized substrate for detecting methylation
ATE522620T1 (de) * 2002-11-14 2011-09-15 Wayne John Cancer Inst Nachweis von micrometastasierung von melanomen und brustkrebs an in paraffin eingegbetteten, von tumoren abführenden lymphknoten mittels quantitativer multimaker rt-pcr
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
US20040101843A1 (en) * 2002-11-22 2004-05-27 Gerald Zon Detection of methylated DNA sites
WO2004050915A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Determination of methylation of nucleic acid sequences
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
JP2006516391A (ja) * 2003-01-29 2006-07-06 エフ．ホフマン−ラ　ロシュ　アーゲー 重亜硫酸塩処理の改良された方法
DE10304219B3 (de) * 2003-01-30 2004-08-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US20090305294A1 (en) * 2003-02-13 2009-12-10 Medimolecular Pty Ltd Method of isolating nucleic acids from stool samples
WO2004087957A2 (en) * 2003-04-03 2004-10-14 Oncomethylome Sciences S.A. Hypermethylated genes and cervical cancer
US20060024676A1 (en) * 2003-04-14 2006-02-02 Karen Uhlmann Method of detecting epigenetic biomarkers by quantitative methyISNP analysis
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150626B2 (en) * 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US20050026183A1 (en) * 2003-05-15 2005-02-03 Jian-Bing Fan Methods and compositions for diagnosing conditions associated with specific DNA methylation patterns
WO2004111266A1 (en) * 2003-06-17 2004-12-23 Human Genetic Signatures Pty Ltd Methods for genome amplification
EP1636386B1 (de) * 2003-06-23 2015-08-05 Epigenomics AG Verfahren und nukleinsäuren für analysen von störungen der proliferation von kolorektalzellen
ATE494390T1 (de) * 2003-06-23 2011-01-15 Epigenomics Ag Verfahren und nukleinsäuren zur analyse von störungen der proliferation von kolonzellen
EP2354250A1 (de) 2003-06-23 2011-08-10 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von Störungen der Proliferation kolorektaler Zellen
US20040265833A1 (en) * 2003-06-23 2004-12-30 Cathy Lofton-Day Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
EP1649050A2 (de) * 2003-06-24 2006-04-26 Epigenomics AG Verbesserter heavymethyl-test zur methylierungsanalyse des gstpi-gens
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
GB0318110D0 (en) * 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
DE10338308B4 (de) * 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
CA2537810C (en) * 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
CN1930303B (zh) * 2003-10-08 2013-11-20 波士顿大学信托人 染色体异常的产前诊断试剂盒
DE602004021192D1 (de) 2003-10-09 2009-07-02 Epigenomics Ag Verbesserte bisulfitkonversion von dna
CA2542542C (en) * 2003-10-21 2015-09-15 Orion Genomics Llc Method of detecting a quantity of methylation at a locus
EP3269826B1 (de) 2003-12-01 2020-03-11 Epigenomics AG Verfahren und nukleinsäuren zur analyse der genexpression, die mit der entwicklung von prostatazellproliferationserkrankungen assoziiert ist
US7501240B2 (en) 2003-12-02 2009-03-10 Roche Molecular Systems, Inc. Method for bisulfite treatment
ATE498693T1 (de) * 2003-12-11 2011-03-15 Epigenomics Ag Marker zur prognose der reaktion auf therapie bzw. der überlebensrate von brustkrebspatienten
EP1561821B1 (de) 2003-12-11 2011-02-16 Epigenomics AG Marker zur Prognose der Reaktion auf Therapie bzw. der Überlebensrate von Brustkrebspatienten
WO2005076837A2 (en) * 2004-02-10 2005-08-25 Cornell Research Foundation, Inc. Method for detection of promoter methylation status
WO2005085477A1 (en) * 2004-03-02 2005-09-15 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
ATE399884T1 (de) * 2004-03-24 2008-07-15 Applera Corp Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden
WO2005098035A2 (en) * 2004-04-06 2005-10-20 Epigenomics Ag Method for the quantification of methylated dna
EP1655377A1 (de) * 2004-04-06 2006-05-10 Epigenomics AG Verfahren zur Quantifizierung methylierter DNA
US20050233340A1 (en) * 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
US7427479B2 (en) * 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
JPWO2005116254A1 (ja) * 2004-05-25 2008-04-03 財団法人ヒューマンサイエンス振興財団 Ｄｎａのメチル化を定量的に評価する方法
ATE491041T1 (de) * 2004-06-23 2010-12-15 Epigenomics Ag Verfahren zur quantifizierung von methylierter dns
US7943308B2 (en) * 2004-06-23 2011-05-17 Epigenomics Ag Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders
US20060234252A1 (en) * 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
US20090023137A1 (en) * 2004-07-16 2009-01-22 Oncomethylome Sciences S.A. ESR1 and Cervical Cancer
EP1769086A2 (de) 2004-07-18 2007-04-04 Epigenomics AG Epigenetische verfahren und nukleinsäuren zum nachweis von proliferativen erkrankungen von brustzellen
EP1632578A1 (de) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
ATE476439T1 (de) * 2004-09-10 2010-08-15 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
US20060068430A1 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
US20080171318A1 (en) * 2004-09-30 2008-07-17 Epigenomics Ag Epigenetic Methods and Nucleic Acids for the Detection of Lung Cell Proliferative Disorders
US7658288B2 (en) * 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP1815026A2 (de) * 2004-11-10 2007-08-08 University Of Southern California Verfahren mit hoher durchlaufleistung mit analyse der dna-methylierung repetitiver elemente
US20090197250A1 (en) * 2004-12-02 2009-08-06 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders
EP1828411B1 (de) * 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Verfahren zur vereinfachung mikrobieller nukleinsäuren durch chemische modifikation von cytosinen
CA2592993C (en) 2004-12-13 2013-06-18 National University Corporation Okayama University Method for detecting methylation in genes and method for examining neoplasm through detecting methylation in genes
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
CN101142323A (zh) * 2004-12-23 2008-03-12 人类遗传标记控股有限公司 人类乳头瘤病毒的检测
WO2006088940A2 (en) * 2005-02-14 2006-08-24 The Johns Hopkins University Neoplasia screening compositions and methods of use
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
EP2385136A1 (de) 2005-04-01 2011-11-09 Epigenomics AG Verbesserte Bisulfit-Umwandlung von DNA
US7749702B2 (en) 2005-04-15 2010-07-06 Epigenomics Ag Methods and nucleic acids for the analyses of cellular proliferative disorders
WO2006119434A2 (en) 2005-05-02 2006-11-09 University Of Southern California DNA METHYLATION MARKERS ASSOCIATED WITH THE CpG ISLAND METHYLATOR PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER
KR100673811B1 (ko) 2005-05-11 2007-01-24 한국표준과학연구원 유전자 내 메틸 시토신의 정량 분석방법 및 이의 사용용도
JP2008541705A (ja) * 2005-05-26 2008-11-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 非標準塩基を含むプライマーを使用する等温鎖置換増幅
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients
US20070037184A1 (en) * 2005-06-16 2007-02-15 Applera Corporation Methods and kits for evaluating dna methylation
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP1904649A2 (de) * 2005-07-18 2008-04-02 Epigenomics AG Zusammenstellung und verfahren zur krebsdiagnostik mit hilfe von pan-krebs-marker
DE502005008746D1 (de) 2005-07-21 2010-02-04 Epigenomics Ag Verfahren zur Quantifizierung methylierter DNA
ATE531820T1 (de) * 2005-09-14 2011-11-15 Human Genetic Signatures Pty Gesundheitszustandstest
EP1945806B1 (de) * 2005-09-21 2013-02-27 Epigenomics AG Marker zur vorhersage des ausgangs einer anthracyclin-behandlung
US20090191548A1 (en) 2005-09-29 2009-07-30 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with tissue classification
WO2007039290A2 (en) * 2005-10-03 2007-04-12 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of cell proliferative disorders
WO2007044780A2 (en) * 2005-10-07 2007-04-19 Emory University Methods and systems for screening for and diagnosing dna methylation associated abnormalities and sex chromosome aneuploidies
WO2007042041A1 (en) * 2005-10-12 2007-04-19 Scanvaegt International A/S Device for transfer of items
US20070087358A1 (en) * 2005-10-19 2007-04-19 Melanie Ehrlich Methods for diagnosing cancer based on DNA methylation status in NBL2
EP1951911A2 (de) 2005-11-08 2008-08-06 Euclid Diagnostics LLC Materialien und verfahren zur prüfung auf methylierung von mit genen assoziierten cpg-inseln bei der diagnostizierung von krebs
WO2007057231A1 (en) 2005-11-17 2007-05-24 Epigenomics Ag Method for the determination of the dna methylation level of a cpg position in identical cells within a tissue sample
CA2631829C (en) * 2005-12-14 2012-01-24 F. Hoffmann-La Roche Ag New method for bisulfite treatment
CA2629824A1 (en) * 2005-12-16 2007-06-21 University Of Maryland, Baltimore Methylation of gene promoters as a predictor of effectiveness of therapy
DE102006001161A1 (de) * 2006-01-06 2007-07-12 Qiagen Gmbh Verfahren zum Nachweis von Cytosin-Methylierungen
WO2007088744A1 (ja) * 2006-02-03 2007-08-09 The University Of Tokyo ゲノムdnaのメチル化状況に基づく細胞の同定方法
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
EP1840224A1 (de) 2006-03-29 2007-10-03 Pangaea Biotech, S.A. Verfahren zur Vorhersage des Überlebens einer chemotherapeutischen Behandlung bei Patienten mit Nichtkleinzelligem Lungenkrebs
EP2011068A4 (de) * 2006-03-30 2010-06-23 Univ Maryland Methylierung von genen als prädiktor für polypenbildung und rezidiv
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP2013360A2 (de) 2006-04-17 2009-01-14 Epigenomics AG Verfahren und nukleinsäuren zum nachweis von proliferativen störungen kolorektaler zellen
US20090317810A1 (en) * 2006-04-17 2009-12-24 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US20080003609A1 (en) * 2006-05-10 2008-01-03 The Cleveland Clinic Foundation Method of detecting bladder urothelial carcinoma
KR100761031B1 (ko) 2006-05-27 2007-10-04 김연수 디엔에이 메틸화 현상을 이용한 암 진단용 키트
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
CA2657576C (en) 2006-07-14 2023-10-31 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
US20110171637A1 (en) * 2006-07-21 2011-07-14 Reimo Tetzner Methods and nucleic acids for analyses of cellular proliferative disorders
ES2546182T3 (es) 2006-07-21 2015-09-21 Epigenomics Ag Métodos y kits para análisis de metilación en trastornos proliferativos celulares colorrectales
EP2049684B1 (de) * 2006-08-08 2015-05-06 Epigenomics AG Verfahren zur methylierungsanalyse von nukleinsäure
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
US20100028877A1 (en) 2006-10-18 2010-02-04 Philipp Schatz Molecule for providing a standard for the quantitative analysis of the methylations status of a nucleic acid
WO2008061801A1 (en) 2006-11-24 2008-05-29 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法
PL2258871T3 (pl) 2007-01-19 2014-10-31 Epigenomics Ag Sposoby i kwasy nukleinowe do analizy zaburzeń proliferacyjnych komórki
US20090203011A1 (en) * 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
WO2008091913A2 (en) * 2007-01-23 2008-07-31 Sequenom, Inc. Method for accurate assessment of dna quality after bisulfite treatment
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
CA2680426A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
WO2008128198A2 (en) * 2007-04-12 2008-10-23 Usc Stevens - University Of Southern California Dna methylation analysis by digital bisulfite genomic sequencing and digital methylight
EP2409700A1 (de) 2007-05-08 2012-01-25 Schering Corporation Verfahren zur Behandlung mit intravenösen Formulierungen mit Temozolomid
WO2009006543A1 (en) 2007-07-02 2009-01-08 Euclid Diagnostics Llc Methods for evaluating the methylation status of a polynucleotide
US20090042290A1 (en) * 2007-08-06 2009-02-12 Christopher Steele Method of modifying a macromolecule without prior extraction from a sample
EP2201131B8 (de) * 2007-09-17 2014-12-10 MDxHealth SA Verbesserter methylierungsnachweis
WO2009059327A2 (en) * 2007-11-02 2009-05-07 University Of Southern California (Usc) Compositions and methods comprising biomarkers of sperm quality, semen quality and fertility
EP2215250B1 (de) * 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzyme zur amplifikation und zum kopieren bisulphit-modifizierter nukleinsäuren
EP2229456A2 (de) 2007-12-11 2010-09-22 Epigenomics AG Verfahren und nukleinsäuren für analysen von lungenkrebs
JP2011505845A (ja) * 2007-12-20 2011-03-03 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 核酸増幅に伴う汚染物質の排除
DE102008008313A1 (de) 2008-02-07 2009-08-13 Qiagen Gmbh Amplifikation von bisulfitierten Nukleinsäuren
AU2009246180B2 (en) 2008-05-14 2015-11-05 Dermtech International Diagnosis of melanoma and solar lentigo by nucleic acid analysis
WO2009153667A2 (en) 2008-06-17 2009-12-23 Occure Gmbh Method for the detection of ovarian cancer
US20100021902A1 (en) * 2008-06-27 2010-01-28 Dimo Dietrich Method for methylation-selective amplification
WO2010003153A2 (en) * 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
US8361746B2 (en) 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
US8541207B2 (en) * 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
US20100136559A1 (en) * 2008-12-02 2010-06-03 Bio-Rad Laboratories, Inc. Chromatin structure detection
WO2010065916A1 (en) * 2008-12-04 2010-06-10 Rush University Medical Center Dna methylation based test for monitoring efficacy of treatment
WO2010083046A2 (en) * 2009-01-15 2010-07-22 The Salk Institute For Biological Studies Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
AR077238A1 (es) 2009-06-26 2011-08-10 Unilever Nv Composiciones para el cuidado bucal. uso de un polimero para fabricar la composicion
ES2534200T3 (es) 2009-08-03 2015-04-20 Epigenomics Ag Métodos para la preservación de la complejidad de la secuencia del ADN genómico
EP2287335A1 (de) * 2009-08-05 2011-02-23 DiaSorin S.p.A. Hoch empfindliches schnelles isothermisches Verfahren zur Erkennung von Methylierungsstrukturen
US20110046009A1 (en) * 2009-08-24 2011-02-24 Perkinelmer Health Sciences, Inc. Methods for detecting dna methylation using encoded particles
CA2777906A1 (en) 2009-10-28 2011-05-05 Signature Diagnostics Ag Method for the prognosis of ovarian carcinoma
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
US8568984B2 (en) * 2010-02-19 2013-10-29 Philadelphia Health & Education Corporation Methods of diagnosing non-urinary tract diseases by detecting aberrant methylation
EP3508854A1 (de) 2010-04-27 2019-07-10 The Regents of The University of California Krebsbiomarker und verfahren zur verwendung davon
ES2581594T3 (es) * 2010-05-25 2016-09-06 The Johns Hopkins University Composiciones y métodos para detectar una neoplasia
WO2012034013A2 (en) 2010-09-10 2012-03-15 Bio-Rad Laboratories, Inc. Detection of rna-interacting regions in dna
EP3034629B1 (de) 2010-09-13 2019-09-04 Clinical Genomics Pty Ltd Krebsdiagnose mit hilfe von methylierungsmarkern
US10428390B2 (en) * 2010-12-16 2019-10-01 Genomictree, Inc. Method for detecting methylation of colorectal cancer specific methylation marker gene for colorectal cancer diagnosis
CA2826748C (en) 2011-02-09 2020-08-04 Bio-Rad Laboratories, Inc. Method of detecting variations in copy number of a target nucleic acid
US20120208193A1 (en) 2011-02-15 2012-08-16 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
WO2012162139A1 (en) 2011-05-20 2012-11-29 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
CN108048573A (zh) 2011-07-08 2018-05-18 表观基因组股份有限公司 用于确定癌症对象之预后的方法和核酸
WO2013019960A1 (en) 2011-08-03 2013-02-07 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
NZ621638A (en) 2011-08-25 2016-03-31 Clinical Genomics Pty Ltd Dna methylation in colorectal and breast cancer diagnostic methods
JP6297490B2 (ja) 2011-08-31 2018-03-20 ジェネンテック， インコーポレイテッド 診断マーカー
NZ622418A (en) 2011-09-07 2014-12-24 Human Genetic Signatures Pty Molecular detection assay
SG11201400996SA (en) 2011-09-30 2014-04-28 Genentech Inc Diagnostic methylation markers of epithelial or mesenchymal phenotype and response to egfr kinase inhibitor in tumours or tumour cells
US10131953B2 (en) 2011-11-03 2018-11-20 Wisconsin Alumni Research Foundation Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
EP2788505B1 (de) 2011-12-06 2017-08-23 MDxHealth SA Verfahren zum nachweis von mutationen und epigenetischen veränderungen
WO2013096661A1 (en) 2011-12-22 2013-06-27 Illumina, Inc. Methylation biomarkers for ovarian cancer
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
NZ701527A (en) 2012-05-11 2016-07-29 Clinical Genomics Pty Ltd Diagnostic gene marker panel for colorectal cancer
WO2013170191A1 (en) 2012-05-11 2013-11-14 Genentech, Inc. Methods of using antagonists of nad biosynthesis from nicotinamide
EP2850212B1 (de) 2012-05-18 2020-01-01 Clinical Genomics Pty. Ltd. Screening-verfahren für kolorektalkarzinom
AU2012380717B2 (en) 2012-05-24 2018-08-16 Fundacio Institucio Catalana De Recerca I Estudis Avancats Method for the identification of the origin of a cancer of unknown primary origin by methylation analysis
CA2882185A1 (en) * 2012-08-28 2014-03-06 Istat Biomedical Co., Ltd. Test composition for screening cancers
US10865446B2 (en) 2012-11-02 2020-12-15 The Johns Hopkins University DNA methylation biomarkers of post-partum depression risk
WO2014093714A1 (en) 2012-12-14 2014-06-19 Bio-Rad Laboratories, Inc. Methods and compositions for using oils for analysis and detection of molecules
WO2014134548A2 (en) * 2013-02-28 2014-09-04 Lu Jim Z Assay, methods and compositions for diagnosing cancer
US9701999B2 (en) 2013-03-14 2017-07-11 Abbott Molecular, Inc. Multiplex methylation-specific amplification systems and methods
US20140274757A1 (en) 2013-03-14 2014-09-18 Marie K. Kirby Differential Methylation Level of CpG Loci That Are Determinative of a Biochemical Reoccurrence of Prostate Cancer
WO2014151551A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
ES2820732T3 (es) 2013-03-28 2021-04-22 Wisconsin Alumni Res Found Marcadores de metilación de ADN no sesgados que definen un defecto de campo amplio en tejidos de próstata histológicamente normales asociados al cáncer de próstata: nuevos biomarcadores para hombres con cáncer de próstata
EP2986762B1 (de) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digitale analyse von analyten
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
CA2918054C (en) 2013-07-11 2022-12-13 The Johns Hopkins University A dna methylation and genotype specific biomarker of suicide attempt and/or suicide ideation
GB201402644D0 (en) * 2014-02-14 2014-04-02 Base4 Innovation Ltd Methylation detection method
MY193347A (en) 2014-05-02 2022-10-06 Malaysian Palm Oil Board Mantle phenotype detection in palm
EP2942401A1 (de) 2014-05-09 2015-11-11 Lifecodexx AG Nachweis von aus einem spezifischen Zelltyp stammender DNA
AU2015257654A1 (en) 2014-05-09 2016-11-10 Lifecodexx Ag Detection of DNA that originates from a specific cell-type and related methods
EP2942400A1 (de) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex-Nachweis von DNA, die aus einem spezifischen Zelltyp herrührt
WO2015181804A2 (en) * 2014-05-30 2015-12-03 Pontificia Universidad Católica De Chile Method and assay for the non-invasive detection of early gastric cancer by the combined use of methylated reprimo cell-free dna and pepsinogen i/ii
KR20170012552A (ko) * 2014-06-05 2017-02-02 클리니칼 게노믹스 피티와이. 엘티디. 메틸화 분석 방법
US11072828B2 (en) 2014-10-06 2021-07-27 The Johns Hopkins University DNA methylation and genotype specific biomarker for predicting post-traumatic stress disorder
US10640831B2 (en) 2014-10-17 2020-05-05 The Regents Of The University Of Colorado, A Body Corporate Biomarkers for head and neck cancer and methods of their use
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
US9984201B2 (en) 2015-01-18 2018-05-29 Youhealth Biotech, Limited Method and system for determining cancer status
EP3168309B8 (de) 2015-11-10 2020-06-03 Eurofins LifeCodexx GmbH Nachweis von fötalen chromosomalen aneuploidien mittels dna-regionen mit unterschiedlicher methylierung zwischen dem fötus und der schwangeren frau
EP3708587A1 (de) 2015-11-27 2020-09-16 Cartherics Pty. Ltd. Genetisch modifizierte zellen und verwendung davon
WO2017106481A1 (en) 2015-12-17 2017-06-22 Illumina, Inc. Distinguishing methylation levels in complex biological samples
WO2017136482A1 (en) 2016-02-01 2017-08-10 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
EP3464642A4 (de) 2016-05-31 2020-02-19 The Regents of the University of California Verfahren zur bewertung, überwachung und modulation des alterungsprozesses
US10093986B2 (en) 2016-07-06 2018-10-09 Youhealth Biotech, Limited Leukemia methylation markers and uses thereof
WO2018009707A1 (en) 2016-07-06 2018-01-11 Youhealth Biotech, Limited Solid tumor methylation markers and uses thereof
US11396678B2 (en) 2016-07-06 2022-07-26 The Regent Of The University Of California Breast and ovarian cancer methylation markers and uses thereof
US11130998B2 (en) 2016-11-14 2021-09-28 Wisconsin Alumni Research Foundation Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
DK3336197T3 (da) 2016-12-16 2022-07-11 Eurofins Genomics Europe Sequencing GmbH Epigenetiske markører og relaterede fremgangsmåder og midler til detektion og behandling af ovariekræft
BR112019018272A2 (pt) 2017-03-02 2020-07-28 Youhealth Oncotech, Limited marcadores metilação para diagnosticar hepatocelular carcinoma e câncer
EP3655418A4 (de) 2017-06-22 2021-05-19 Triact Therapeutics, Inc. Verfahren zur behandlung von glioblastom
US11628144B2 (en) 2017-09-29 2023-04-18 Triact Therapeutics, Inc. Iniparib formulations and uses thereof
WO2020056338A1 (en) 2018-09-14 2020-03-19 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
SG11202107670TA (en) 2019-01-18 2021-08-30 Univ California Dna methylation measurement for mammals based on conserved loci
US11578373B2 (en) 2019-03-26 2023-02-14 Dermtech, Inc. Gene classifiers and uses thereof in skin cancers
US11542548B2 (en) 2019-06-11 2023-01-03 Wisconsin Alumni Research Foundation; Blood DNA methylation biomarker diagnostic test for anxiety and depressive disorders
CN112662761A (zh) * 2020-03-05 2021-04-16 博尔诚（北京）科技有限公司 一种检测3种实质性器官肿瘤的探针组合物
US20230203585A1 (en) 2020-05-22 2023-06-29 Wisconsin Alumni Research Foundation Epigenetic modification of select genes as markers of hypersomnolence
WO2023052640A1 (en) 2021-09-30 2023-04-06 Tivenix Sa A method for diagnosing and predicting progression of neurodegenerative diseases or disorders
WO2023066972A1 (en) 2021-10-19 2023-04-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Dna methylation signature for diagnosing hepatocellular carcinoma
EP4170661A1 (de) 2021-10-19 2023-04-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Methylierungsprofilanalyse mittels glättungsverfahren
WO2023175019A1 (en) 2022-03-15 2023-09-21 Genknowme S.A. Method determining the difference between the biological age and the chronological age of a subject
WO2024044578A1 (en) 2022-08-22 2024-02-29 University Of Virginia Dna methylation biomarkers of premenstrual dysphoric disorder and perimenopausal depression
WO2024059649A1 (en) 2022-09-13 2024-03-21 The Saban Research Institute; Children's Hospital Los Angeles Dna methylation signature of retinoblastoma

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5788146A (en) * 1996-02-13 1998-08-04 Bradford Company Parent welding partition assembly
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
EP1179600B1 (de) 1996-06-04 2005-05-11 University Of Utah Research Foundation Überwachung der Hybridisierung während PCR
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AUPP312998A0 (en) 1998-04-23 1998-05-14 Commonwealth Scientific And Industrial Research Organisation Diagnostic assay
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
ATE498693T1 (de) 2003-12-11 2011-03-15 Epigenomics Ag Marker zur prognose der reaktion auf therapie bzw. der überlebensrate von brustkrebspatienten
WO2005098035A2 (en) 2004-04-06 2005-10-20 Epigenomics Ag Method for the quantification of methylated dna
US20090197250A1 (en) 2004-12-02 2009-08-06 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders

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