DE60029209D1 - Vorrichtung und verfahren zur einbringung von substanzen in eine zelle durch elektroporation - Google Patents

Vorrichtung und verfahren zur einbringung von substanzen in eine zelle durch elektroporation

Info

Publication number
DE60029209D1
DE60029209D1 DE60029209T DE60029209T DE60029209D1 DE 60029209 D1 DE60029209 D1 DE 60029209D1 DE 60029209 T DE60029209 T DE 60029209T DE 60029209 T DE60029209 T DE 60029209T DE 60029209 D1 DE60029209 D1 DE 60029209D1
Authority
DE
Germany
Prior art keywords
cell
electroporation
transfected
substance
introducing substances
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60029209T
Other languages
English (en)
Other versions
DE60029209T2 (de
Inventor
John Dodgson
Robert Corless
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Q FUTURES LTD., CHESTER, CHESHIRE, GB
Original Assignee
AstraZeneca AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AstraZeneca AB filed Critical AstraZeneca AB
Publication of DE60029209D1 publication Critical patent/DE60029209D1/de
Application granted granted Critical
Publication of DE60029209T2 publication Critical patent/DE60029209T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
DE60029209T 1999-04-16 2000-04-17 Vorrichtung und verfahren zur einbringung von substanzen in eine zelle durch elektroporation Expired - Fee Related DE60029209T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9908681.1A GB9908681D0 (en) 1999-04-16 1999-04-16 Apparatus for, and method of, introducing a substance into an object
GB9908681 1999-04-16
PCT/GB2000/001477 WO2000063408A2 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into a cell by electroporation

Publications (2)

Publication Number Publication Date
DE60029209D1 true DE60029209D1 (de) 2006-08-17
DE60029209T2 DE60029209T2 (de) 2007-06-06

Family

ID=10851623

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60029209T Expired - Fee Related DE60029209T2 (de) 1999-04-16 2000-04-17 Vorrichtung und verfahren zur einbringung von substanzen in eine zelle durch elektroporation

Country Status (9)

Country Link
US (2) US6653136B1 (de)
EP (1) EP1173599B1 (de)
JP (1) JP2004500022A (de)
AT (1) ATE332387T1 (de)
AU (2) AU4582100A (de)
DE (1) DE60029209T2 (de)
ES (1) ES2267518T3 (de)
GB (1) GB9908681D0 (de)
WO (2) WO2000063407A2 (de)

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WO2002103331A1 (de) * 2001-06-15 2002-12-27 Zeptosens Ag Körper für durchflussküvetten und deren verwendung
WO2003093791A2 (en) * 2002-05-03 2003-11-13 The Regents Of The University Of California Fast electrical lysis of cells and rapid collection of the contents thereof using capillary electrophoresis
AU2003249595A1 (en) * 2002-07-16 2004-02-02 National Institute Of Agrobiological Sciences Electroporation method including the use of depressurization/pressurization
EP1565555A4 (de) * 2002-09-30 2008-07-09 Maxcyte Inc Vorrichtung und verfahren zur strämungselektroporation
US7087444B2 (en) * 2002-12-16 2006-08-08 Palo Alto Research Center Incorporated Method for integration of microelectronic components with microfluidic devices
JP2004305076A (ja) * 2003-04-04 2004-11-04 Canon Inc 対象物に対する修飾装置
JP2004344036A (ja) * 2003-05-21 2004-12-09 Fujitsu Ltd 物質導入装置及び物質導入システム
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
US20050118705A1 (en) * 2003-11-07 2005-06-02 Rabbitt Richard D. Electrical detectors for microanalysis
JP2005278480A (ja) * 2004-03-29 2005-10-13 Fujitsu Ltd 物質導入装置及び物質導入用チップ
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
MXPA06014461A (es) * 2004-06-12 2007-05-23 Digital Biotechnology Co Ltd Electroporador que tiene un miembro hueco alargado.
US7592139B2 (en) 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
JP4894199B2 (ja) * 2005-02-17 2012-03-14 富士通株式会社 物質注入装置
US7704743B2 (en) * 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
EP1734111B1 (de) * 2005-06-13 2020-06-24 Tosoh Corporation Zellfusionsvorrichtung und Verfahren zur Zellfusion unter Verwendung derselben
JP4659553B2 (ja) * 2005-08-05 2011-03-30 富士通株式会社 自動マイクロインジェクション装置および細胞捕捉プレート
US20070105206A1 (en) * 2005-10-19 2007-05-10 Chang Lu Fluidic device
US8226811B2 (en) * 2005-11-02 2012-07-24 May-Ruben Technologies, Inc. High impedance system for generating electric fields and method of use
AU2006342101A1 (en) * 2005-12-07 2007-10-25 Genetronics, Inc Variable volume electroporation chamber and methods therefore
JP5040191B2 (ja) * 2006-06-29 2012-10-03 富士通株式会社 マイクロインジェクション装置及び自動焦点調整方法
US20080138876A1 (en) * 2006-12-11 2008-06-12 Bio-Rad Laboratories, Inc. Transfection in electronically driven continuous flow
US9110010B2 (en) * 2007-05-11 2015-08-18 The United States Of America, As Represented By The Secretary Of The Navy Electrical detection using confined fluids
US8961877B2 (en) * 2007-08-09 2015-02-24 Massachusetts Institute Of Technology High-throughput, whole-animal screening system
US8308926B2 (en) 2007-08-20 2012-11-13 Purdue Research Foundation Microfluidic pumping based on dielectrophoresis
WO2009032827A2 (en) * 2007-09-04 2009-03-12 Purdue Research Foundation Electroporative flow cytometry
JP5226352B2 (ja) * 2008-03-21 2013-07-03 オリンパス株式会社 生体観察装置及び生体観察方法
KR101084528B1 (ko) 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. 전기천공 장치용 파이펫 팁
US20090280518A1 (en) * 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
DE102010031103A1 (de) * 2010-07-08 2012-01-12 Robert Bosch Gmbh Verfahren zur Herstellung eines integrierten mikrofluidischen Systems und integriertes mikrofluidisches System
WO2012147463A1 (ja) * 2011-04-28 2012-11-01 株式会社日立製作所 細胞培養容器および細胞培養装置
WO2013059717A1 (en) * 2011-10-21 2013-04-25 Nanoinjection Technologies, L.L.C. Systems and devices for restraining a micro-object and associated methods
GB201312035D0 (en) 2013-07-04 2013-08-21 Cytomos Ltd Biological sensing apparatus
EP3260163B1 (de) * 2014-05-02 2019-07-17 Lonza Cologne GmbH Vorrichtung und verfahren zur grossvolumigen transfektion
EP3096338B1 (de) 2015-05-21 2019-07-24 Nokia Technologies Oy Vorrichtung und verfahren zur bereitstellung einer zeitvariablen spannung
EP3096134B1 (de) * 2015-05-21 2019-07-24 Nokia Technologies Oy Vorrichtung und verfahren zur bereitstellung einer zeitvariablen spannung
EP3138920B1 (de) * 2015-09-07 2018-06-13 Miltenyi Biotec GmbH Einwegkartusche für elektroporation
US11225638B2 (en) * 2016-04-04 2022-01-18 CyteQuest, Inc. System, device and method for electroporation of cells
US20170299571A1 (en) 2016-04-14 2017-10-19 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for determining mechanical properties of cells
US11198841B2 (en) 2017-06-09 2021-12-14 Hewlett-Packard Development Company, L.P. Porated cell ejection devices
US11215562B2 (en) 2017-08-28 2022-01-04 Hewlett-Packard Development Company, L.P. Deformable covers on sensors and reservoirs
EP3666881A1 (de) * 2018-12-12 2020-06-17 AIT Austrian Institute of Technology GmbH Gekoppelte sortierung und elektrische behandlung biologischer zellen
WO2021010341A1 (ja) 2019-07-12 2021-01-21 富士フイルム株式会社 生体由来物の製造方法、産生物の製造方法及び電圧印加装置
WO2021206686A1 (en) * 2020-04-07 2021-10-14 Hewlett-Packard Development Company, L.P. Microfluidic chip cell sorting and transfection
WO2024015072A1 (en) * 2022-07-15 2024-01-18 Hewlett-Packard Development Company, L.P. Cell porating and optically detecting microfluidic devices

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JP2662215B2 (ja) * 1986-11-19 1997-10-08 株式会社日立製作所 細胞保持装置
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US4822470A (en) * 1987-10-09 1989-04-18 Baylor College Of Medicine Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
US5137817A (en) * 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5173158A (en) * 1991-07-22 1992-12-22 Schmukler Robert E Apparatus and methods for electroporation and electrofusion
US5874268A (en) * 1996-09-23 1999-02-23 Duke University Method of introducing exogenous compounds into cells by electroporation and apparatus for same
US6090617A (en) * 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
WO1999011771A1 (en) * 1997-09-04 1999-03-11 Science Research Laboratory, Inc. Cell separation using electric fields
AU785026B2 (en) * 1998-12-04 2006-08-24 Genetronics, Inc. Facs assisted methods for introducing individual chromosomes into cells
DE19859459A1 (de) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Mikrosysteme zur Zellpermeation und Zellfusion
US6193647B1 (en) * 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method

Also Published As

Publication number Publication date
WO2000063408A2 (en) 2000-10-26
GB9908681D0 (en) 1999-06-09
AU4129600A (en) 2000-11-02
JP2004500022A (ja) 2004-01-08
WO2000063407A3 (en) 2001-01-25
US20040106189A1 (en) 2004-06-03
AU4582100A (en) 2000-11-02
ATE332387T1 (de) 2006-07-15
US6653136B1 (en) 2003-11-25
WO2000063408A3 (en) 2001-01-25
ES2267518T3 (es) 2007-03-16
EP1173599A2 (de) 2002-01-23
EP1173599B1 (de) 2006-07-05
DE60029209T2 (de) 2007-06-06
WO2000063407A2 (en) 2000-10-26

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: Q FUTURES LTD., CHESTER, CHESHIRE, GB

8328 Change in the person/name/address of the agent

Representative=s name: JANNIG & REPKOW PATENTANWAELTE, 86199 AUGSBURG

8339 Ceased/non-payment of the annual fee