DE60024618D1 - Einzelschicht und elektrode zur feststellung von markierten molekülen und verfahren zu deren verwendung - Google Patents

Einzelschicht und elektrode zur feststellung von markierten molekülen und verfahren zu deren verwendung

Info

Publication number
DE60024618D1
DE60024618D1 DE60024618T DE60024618T DE60024618D1 DE 60024618 D1 DE60024618 D1 DE 60024618D1 DE 60024618 T DE60024618 T DE 60024618T DE 60024618 T DE60024618 T DE 60024618T DE 60024618 D1 DE60024618 D1 DE 60024618D1
Authority
DE
Germany
Prior art keywords
nucleic acid
electrode
oxidation
reduction reaction
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60024618T
Other languages
English (en)
Inventor
E Eckhardt
C Mikulecky
E Napier
S Thomas
Holden Thorp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of North Carolina at Chapel Hill
University of North Carolina System
Original Assignee
University of North Carolina at Chapel Hill
University of North Carolina System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of North Carolina at Chapel Hill, University of North Carolina System filed Critical University of North Carolina at Chapel Hill
Application granted granted Critical
Publication of DE60024618D1 publication Critical patent/DE60024618D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
DE60024618T 1999-04-22 2000-02-04 Einzelschicht und elektrode zur feststellung von markierten molekülen und verfahren zu deren verwendung Expired - Lifetime DE60024618D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/296,929 US6127127A (en) 1995-06-27 1999-04-22 Monolayer and electrode for detecting a label-bearing target and method of use thereof
PCT/US2000/002976 WO2000065099A1 (en) 1999-04-22 2000-02-04 Monolayer and electrode for detecting a label-bearing target and method of use thereof

Publications (1)

Publication Number Publication Date
DE60024618D1 true DE60024618D1 (de) 2006-01-12

Family

ID=23144136

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60024618T Expired - Lifetime DE60024618D1 (de) 1999-04-22 2000-02-04 Einzelschicht und elektrode zur feststellung von markierten molekülen und verfahren zu deren verwendung

Country Status (13)

Country Link
US (1) US6127127A (de)
EP (1) EP1185692B1 (de)
JP (1) JP2002542794A (de)
KR (1) KR20020008157A (de)
CN (1) CN1347460A (de)
AT (1) ATE312200T1 (de)
AU (1) AU781085B2 (de)
CA (1) CA2367594A1 (de)
DE (1) DE60024618D1 (de)
HK (1) HK1045178B (de)
NO (1) NO20014882L (de)
NZ (1) NZ514682A (de)
WO (1) WO2000065099A1 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6207369B1 (en) * 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
US6346387B1 (en) 1995-06-27 2002-02-12 Xanthon, Inc. Detection of binding reactions using labels detected by mediated catalytic electrochemistry
US7815963B2 (en) * 1996-10-17 2010-10-19 The Trustees Of Princeton University Enhanced bonding layers on titanium materials
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US6833267B1 (en) * 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US20020177135A1 (en) * 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
US7348183B2 (en) * 2000-10-16 2008-03-25 Board Of Trustees Of The University Of Arkansas Self-contained microelectrochemical bioassay platforms and methods
AU2002241803A1 (en) * 2000-10-20 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Transient electrical signal based methods and devices for characterizing molecular interaction and/or motion in a sample
US6632536B2 (en) * 2000-12-28 2003-10-14 International Business Machines Corporation Self-assembled monolayer etch barrier for indium-tin-oxide useful in manufacturing thin film transistor-liquid crystal displays
DE10126798A1 (de) * 2001-06-01 2002-12-19 Nanotype Gmbh Verfahren zur Bestimmung einer Probe
US20020182606A1 (en) * 2001-06-04 2002-12-05 Xanthon, Inc. Detection of single nucleotide polymorphisms
US6824974B2 (en) * 2001-06-11 2004-11-30 Genorx, Inc. Electronic detection of biological molecules using thin layers
US20040248282A1 (en) * 2001-06-11 2004-12-09 Pisharody Sobha M. Electronic detection of biological molecules using thin layers
US7842246B2 (en) 2001-06-29 2010-11-30 Meso Scale Technologies, Llc Assay plates, reader systems and methods for luminescence test measurements
WO2003062783A2 (en) * 2001-07-20 2003-07-31 North Carolina State University Light addressable electrochemical detection of duplex structures
JP4262426B2 (ja) * 2001-09-03 2009-05-13 富士フイルム株式会社 核酸断片固定電極及びその利用
EP1425583A1 (de) * 2001-09-12 2004-06-09 Eidgenössische Technische Hochschule Zürich Vorrichtung mit chemisch strukturierten oberflächen
AU2004201425B2 (en) * 2001-09-24 2007-06-28 University Of North Carolina At Chapel Hill Methods for the electrochemical detection of multiple target compounds
JP2005520175A (ja) * 2001-09-24 2005-07-07 ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル 多重標的化合物の電気化学的検出方法
US7202028B2 (en) * 2001-09-24 2007-04-10 The University Of North Carolina At Chapel Hill Methods for the electrochemical detection of multiple target compounds
US20040014078A1 (en) * 2002-02-06 2004-01-22 James Xia Compositions and methods for rolling circle amplification
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
GB0211449D0 (en) * 2002-05-17 2002-06-26 Oxford Biosensors Ltd Analyte measurement
JP4832759B2 (ja) * 2002-12-11 2011-12-07 サントル ナショナル ドゥ ラ ルシェルシュ シアンティフィク プローブ分子と標的生体分子との間の少なくとも１つの特異的相互作用を電子的に検出する方法
US20040180369A1 (en) * 2003-01-16 2004-09-16 North Carolina State University Photothermal detection of nucleic acid hybridization
WO2005016115A2 (en) * 2003-01-23 2005-02-24 Montana State University Biosensors utilizing dendrimer-immobilized ligands and their use thereof
KR100547015B1 (ko) * 2003-05-23 2006-01-26 주식회사 올메디쿠스 일정한 크기 이상의 분석물질을 정량 분석하기 위한바이오센서 및 그 제조방법
WO2005042783A1 (en) * 2003-10-30 2005-05-12 North Carolina State University Temperature-jump enhanced electrochemical detection of nucleic acid hybridization
US20100000881A1 (en) * 2003-10-30 2010-01-07 North Carolina State University Electrochemical detection of nucleic acid hybridization
US7981362B2 (en) 2003-11-04 2011-07-19 Meso Scale Technologies, Llc Modular assay plates, reader systems and methods for test measurements
US8048377B1 (en) 2004-03-08 2011-11-01 Hewlett-Packard Development Company, L.P. Immobilizing chemical or biological sensing molecules on semi-conducting nanowires
US7462452B2 (en) * 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US20060094024A1 (en) * 2004-11-01 2006-05-04 Pirrung Michael C Electrochemical arrays
US20090203547A1 (en) * 2005-02-18 2009-08-13 Albert Banes Gene and Cognate Protein Profiles and Methods to Determine Connective Tissue Markers in Normal and Pathologic Conditions
US20070235346A1 (en) * 2006-04-11 2007-10-11 Popovich Natasha D System and methods for providing corrected analyte concentration measurements
US7909983B2 (en) * 2006-05-04 2011-03-22 Nipro Diagnostics, Inc. System and methods for automatically recognizing a control solution
CN101506380A (zh) * 2006-08-11 2009-08-12 香港科技大学 用电导或电化学活跃标签实时定量分析与监察核酸扩增的方法与系统
US20080131709A1 (en) * 2006-09-28 2008-06-05 Aculon Inc. Composite structure with organophosphonate adherent layer and method of preparing
US8907061B2 (en) 2008-01-11 2014-12-09 Lawrence Livermore National Security, Llc. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification
US20090203549A1 (en) * 2008-02-07 2009-08-13 Hoeprich Jr Paul D Functionalized platform for arrays configured for optical detection of targets and related arrays, methods and systems
US9303273B2 (en) 2008-05-09 2016-04-05 Lawrence Livermore National Security, Llc Nanolipoprotein particles comprising a natural rubber biosynthetic enzyme complex and related products, methods and systems
US8906610B2 (en) 2010-02-08 2014-12-09 The Regents Of The University Of California Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis
EP2661485A4 (de) 2011-01-06 2018-11-21 Meso Scale Technologies, LLC Testkassetten und verfahren zu ihrer verwendung
US9644038B2 (en) 2011-12-21 2017-05-09 The Regents Of The University Of California Apolipoprotein nanodiscs with telodendrimer
WO2016049061A1 (en) 2014-09-22 2016-03-31 Lawrence Livermore National Security, Llc Electrochemical flow-cell for hydrogen production and nicotinamide co-factor dependent target reduction, and related methods and systems
WO2017044899A1 (en) 2015-09-11 2017-03-16 Lawrence Livermore National Security, Llc Synthetic apolipoproteins, and related compositions methods and systems for nanolipoprotein particles formation
KR101862198B1 (ko) * 2016-09-30 2018-05-30 (주)스파크바이오파마 2차원 겔 전기영동에서의 열 안정성 변화-기반 형광 차이를 이용한 표적 단백질의 규명 방법
WO2018204421A2 (en) 2017-05-02 2018-11-08 Lawrence Livermore National Security, Llc Momp telonanoparticles, and related compositions, methods and systems
CN111225791B (zh) * 2017-10-11 2022-04-12 微仙美国有限公司 膦酸酯及其用途

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3278334D1 (en) * 1981-10-23 1988-05-19 Genetics Int Inc Sensor for components of a liquid mixture
EP0149339B1 (de) * 1983-12-16 1989-08-23 MediSense, Inc. Test für Nukleinsäuren
US4704353A (en) * 1984-04-27 1987-11-03 Molecular Devices Corporation Photoresponsive redox detection and discrimination
US4883579A (en) * 1984-04-27 1989-11-28 Molecular Devices Corporation Photoresponsive redox detection and discrimination
GB8417301D0 (en) * 1984-07-06 1984-08-08 Serono Diagnostics Ltd Assay
US5157032A (en) * 1985-01-18 1992-10-20 The Trustees Of Columbia University In The City Of New York Mixed ligand complexes and uses thereof as binding agents and probes to DNA
US5112974A (en) * 1985-01-18 1992-05-12 The Trustees Of Columbia University In The City Of New York Mixed ligand complexes and uses thereof as binding agents to DNA
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8606719D0 (en) * 1986-03-19 1986-04-23 Lister Preventive Med Genetic probes
US5066372A (en) * 1986-05-02 1991-11-19 Ciba Corning Diagnostics Corp. Unitary multiple electrode sensor
US4908307A (en) * 1986-12-19 1990-03-13 Karin D. Rodland Hybridization method and probe for detecting nucleic acid sequences
US4963815A (en) * 1987-07-10 1990-10-16 Molecular Devices Corporation Photoresponsive electrode for determination of redox potential
US4995402A (en) * 1988-10-12 1991-02-26 Thorne, Smith, Astill Technologies, Inc. Medical droplet whole blood and like monitoring
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5156810A (en) * 1989-06-15 1992-10-20 Biocircuits Corporation Biosensors employing electrical, optical and mechanical signals
US5194372A (en) * 1989-07-10 1993-03-16 Hitachi, Ltd. Method and apparatus for detecting disorders in genomic substances
JPH0376600A (ja) * 1989-08-21 1991-04-02 Hitachi Ltd 遺伝子変異検出法
CA2033692A1 (en) * 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
AU7575291A (en) * 1990-04-06 1991-10-30 Syngene, Inc. Process and composition for performing dna assays
US5667976A (en) * 1990-05-11 1997-09-16 Becton Dickinson And Company Solid supports for nucleic acid hybridization assays
DE69125441T2 (de) * 1990-09-28 1997-11-06 Toshiba Kawasaki Kk Verfahren zum Gennachweis
US5776672A (en) * 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
JP3076600B2 (ja) 1990-11-20 2000-08-14 株式会社日本マイクロニクス 表示パネル用プローバ
US5272056A (en) * 1991-01-03 1993-12-21 The Research Foundation Of State University Of New York Modification of DNA and oligonucleotides using metal complexes of polyaza ligands
US5439829A (en) * 1991-01-30 1995-08-08 Eli Lilly And Company Immobilization of biologically active molecules by changing the Oxidation state of a chelated transition metal ion
FR2673289B1 (fr) * 1991-02-21 1994-06-17 Asulab Sa Capteur de mesure de la quantite d'un composant en solution.
US5171853A (en) * 1991-08-05 1992-12-15 North Carolina State University Process of cleaving nucleic acids with oxoruthenium(IV) complexes
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
DK0620822T3 (da) * 1991-11-07 2001-08-27 Nanotronics Inc Hybridisering af polynukleotider konjugeret med kromoforer og fluoroforer til dannelse af et donor-til-donor energioverførselssystem
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
GB9206671D0 (en) * 1992-03-27 1992-05-13 Cranfield Biotech Ltd Electrochemical detection of dna hybridisation
EP0587408A1 (de) * 1992-09-07 1994-03-16 Terumo Kabushiki Kaisha Methode zur Bestimmung von DNS und Sensor hierfür
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
FR2703359B1 (fr) * 1993-03-31 1995-06-23 Cis Bio Int Copolymère nucléotide(s)/polymère conducteur électronique ; son procédé de préparation et son utilisation .
AU7212494A (en) * 1993-06-25 1995-01-17 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
US5494562A (en) * 1994-06-27 1996-02-27 Ciba Corning Diagnostics Corp. Electrochemical sensors
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
IT1275482B (it) * 1995-07-05 1997-08-07 Cooperativa Centro Ricerche Po Biosensori elettrochimici basati su trasduttori compositi
WO1997044651A1 (en) * 1996-05-22 1997-11-27 Australian Membrane And Biotechnology Research Institute Nucleic acid sensor
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
DE69735601T2 (de) * 1996-11-29 2007-10-18 The Board Of Trustees Of The Leland Stanford Junior University, Stanford Anordnungen von unabhängig voneinander ansteuerbaren, gestützten flüssigbilayer-membranen und ihre anwendungsverfahren

Also Published As

Publication number Publication date
JP2002542794A (ja) 2002-12-17
WO2000065099A1 (en) 2000-11-02
CA2367594A1 (en) 2000-11-02
NO20014882L (no) 2001-12-27
NO20014882D0 (no) 2001-10-08
EP1185692B1 (de) 2005-12-07
AU3483000A (en) 2000-11-10
NZ514682A (en) 2003-07-25
KR20020008157A (ko) 2002-01-29
AU781085B2 (en) 2005-05-05
EP1185692A1 (de) 2002-03-13
US6127127A (en) 2000-10-03
ATE312200T1 (de) 2005-12-15
HK1045178B (zh) 2006-08-11
HK1045178A1 (en) 2002-11-15
CN1347460A (zh) 2002-05-01

Similar Documents

Publication Publication Date Title
DE60024618D1 (de) Einzelschicht und elektrode zur feststellung von markierten molekülen und verfahren zu deren verwendung
D'Orazio Biosensors in clinical chemistry—2011 update
Ramanavičius et al. Electrochemical sensors based on conducting polymer—polypyrrole
Du et al. Reagentless, ratiometric electrochemical DNA sensors with improved robustness and reproducibility
Paleček et al. Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor
Paleček et al. Magnetic beads as versatile tools for electrochemical DNA and protein biosensing
Hu et al. Controlled/“living” radical polymerization-based signal amplification strategies for biosensing
Lucarelli et al. Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors
Odenthal et al. An introduction to electrochemical DNA biosensors
Zong et al. Chemiluminescence imaging for a protein assay via proximity-dependent DNAzyme formation
Hu et al. Electrochemical DNA biosensing via electrochemically controlled reversible addition–fragmentation chain transfer polymerization
Hu et al. Electrochemically controlled RAFT polymerization for highly sensitive electrochemical biosensing of protein kinase activity
Mahshid et al. Biomolecular steric hindrance effects are enhanced on nanostructured microelectrodes
Hu et al. Surface-initiated-reversible-addition–fragmentation-chain-transfer polymerization for electrochemical DNA biosensing
CA2505254A1 (en) Universal tag assay
Chen et al. Ultrasensitive electrochemical biosensor developed by probe lengthening for detection of genomic DNA in human serum
Damiati et al. Flex printed circuit board implemented graphene-based DNA sensor for detection of SARS-CoV-2
Han et al. Electrochemical signal amplification for immunosensor based on 3D interdigitated array electrodes
Trifonov et al. Application of the hybridization chain reaction on electrodes for the amplified and parallel electrochemical analysis of DNA
Wan et al. Nanoprobe-initiated enzymatic polymerization for highly sensitive electrochemical DNA detection
Fan et al. Scanning electrochemical microscopy of DNA hybridization on DNA microarrays enhanced by HRP-modified SiO2 nanoparticles
Baek et al. A one-step and label-free, electrochemical DNA detection using metal ion-mediated molecular beacon probe
Gao et al. A novel electrochemical biosensor for DNA detection based on exonuclease III-assisted target recycling and rolling circle amplification
Limoges et al. Theory and practice of enzyme bioaffinity electrodes. Direct electrochemical product detection
Chen et al. DNA nanosieve-based regenerative electrochemical biosensor utilizing nucleic acid flexibility for accurate allele typing in clinical samples

Legal Events

Date Code Title Description
8332 No legal effect for de