DE4327752A1 - Radiation measuring device for luminescence and fluorescence measurement - Google Patents

Radiation measuring device for luminescence and fluorescence measurement

Info

Publication number
DE4327752A1
DE4327752A1 DE19934327752 DE4327752A DE4327752A1 DE 4327752 A1 DE4327752 A1 DE 4327752A1 DE 19934327752 DE19934327752 DE 19934327752 DE 4327752 A DE4327752 A DE 4327752A DE 4327752 A1 DE4327752 A1 DE 4327752A1
Authority
DE
Germany
Prior art keywords
measuring
radiation
luminescence
measuring device
radiation measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19934327752
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Taurus Daten & Mestechnik GmbH
Original Assignee
Taurus Daten & Mestechnik GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Taurus Daten & Mestechnik GmbH filed Critical Taurus Daten & Mestechnik GmbH
Priority to DE19934327752 priority Critical patent/DE4327752A1/en
Publication of DE4327752A1 publication Critical patent/DE4327752A1/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0272—Handheld
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0291—Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts
    • G01N2021/015—Apparatus with interchangeable optical heads or interchangeable block of optics and detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/022—Casings
    • G01N2201/0221—Portable; cableless; compact; hand-held
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/024—Modular construction

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Until now, luminescence and fluorescence measurements have been carried out by means of two corresponding measuring devices. The expenditure in terms of equipment for users of both methods, in particular in the case of rapid analyses on site, is very high. Both measuring methods are intended to be able to be carried out using the new measuring device. A mechanical device (2) having optical (3) and electrical interface (4) is mounted on the end of the radiation measuring device (1), which contains the essential subassemblies such as power supply, detector, indicator, microcontroller, operating keyboard and data interface. Depending on the measurement task, corresponding measuring heads (5, 6) can be coupled to this device (2). This radiation measuring device enables the carrying out of luminescence and fluorescence measurements by simply exchanging the measuring head. Since practically any desired measuring heads, which only need to satisfy the requirement of the optical/electrical interface, can be coupled to this radiation measuring device, the universal use of this device in radiation measuring technology is possible. In particular in the case of portable or hand-held measuring devices, small volumes result, accompanied by the highest flexibility. <IMAGE>

Description

In Bereichen wie Medizin, Hygiene und Umweltschutz werden zunehmend transportable Meßsysteme zum Nachweis chemischer und chemobiologischer Reaktionen eingesetzt.In areas such as medicine, hygiene and environmental protection increasingly portable measuring systems for the detection of chemical and chemobiological reactions.

Unter anderem auf Grund hoher Sensitivität und breiter An­ wendungsmöglichkeiten bewähren sich hier insbesondere Meß­ geräte zum Nachweis von Lumineszenz- und Fluoreszenzreaktio­ nen.Among other things, due to its high sensitivity and wide range Possible applications are particularly useful here devices for the detection of luminescence and fluorescence reactions nen.

In den Patentschriften US 316628, DE 26 06 481 und DE 25 09 215 werden Lumineszenz- und Fluoreszenzmeßgeräte beschrieben. In DE 25 09 215 und DE 26 06 481 ist der Aufbau des Fluorometer­ systems mit monochromatischer Lichtquelle, fluoreszenter Probensubstanz, Lichtleitvorrichtung (DE 25 09 215), Fokkus­ siereinrichtung und Detektor aufgezeigt. Aus US 316628 ist ein Gerät bekannt, welches die Meßmethoden für Fluorometrie und Photometrie beinhaltet. Während die Geräte nach DE 25 09 215 und DE 26 06 481 ausschließlich für ein Meßverfahren, der Fluoreszenzmessung, ausgelegt sind, ist das Gerät nach US 316628 auch als Photometer einsetzbar. Allen in diesen Pa­ tenten vorgestellten Geräten ist jedoch gemeinsam, daß we­ der Probenaufnahmen und Meßverfahren in einfacher Weise ge­ wechselt noch Lumineszenz- und Fluoreszenz- bzw. andere Strahlungsmessungen mit optischem Detektor mit einem Gerät durchgeführt werden können.In the patents US 316628, DE 26 06 481 and DE 25 09 215 Luminescence and fluorescence measuring devices are described. DE 25 09 215 and DE 26 06 481 describe the structure of the fluorometer systems with monochromatic light source, fluorescent Sample substance, light guide device (DE 25 09 215), focus siereinrichtung and detector shown. From US 316628 a device known which the measurement methods for fluorometry and includes photometry. While the devices according to DE 25 09 215 and DE 26 06 481 exclusively for a measuring method that Fluorescence measurement, are designed, the device is according to US 316628 can also be used as a photometer. All in these Pa However, it is common for devices presented that we the sample recordings and measurement methods in a simple manner alternates luminescence and fluorescence or others Radiation measurements with an optical detector with one device can be carried out.

Der im Patentanspruch angegebenen Erfindung liegt das Pro­ blem zugrunde, den gerätetechnischen Aufwand bei Anwendung der Fluorometrie, Luminometrie sowie anderer Strahlungsmeß­ verfahren, die sich mit Hilfe optischer Detektoren durch­ führen lassen, zu verringern und eine Vielzahl unterschied­ licher Probenaufnahmen und Messanordnungen an einem trag­ baren Gerät zu ermöglichen.The invention specified in the claim is the pro blem underlying, the device-related effort when using fluorometry, luminometry and other radiation measurements procedures, which are carried out with the help of optical detectors Let lead, decrease and a lot of difference sample recordings and measuring arrangements on a stretcher allowable device.

Die Lösung hierzu ergibt sich aus dem Patentanspruch. The solution to this arises from the patent claim.  

Die mit der Erfindung erzielten Vorteile bestehen insbeson­ dere darin, daß der Anwender an Stelle mehrerer Meßgeräte für die verschiedenen Meßverfahren nur ein Strahlungsmeßge­ rät benötigt. Die Anpassung des Gerätes an verschiedene Meß­ verfahren erfolgt bei Bedarf durch einfaches Auswechseln des Meßkopfes. Über eine elektrische Schnittstelle des Strah­ lungsmeßgerätes wird der Meßkopftyp detektiert und das be­ treffende Meßregime im Strahlungsmeßgerät eingestellt.The advantages achieved with the invention are in particular the fact that the user instead of several measuring devices only one radiation measurement for the various measurement methods advises needed. The adaptation of the device to different measuring If necessary, the procedure is carried out by simply changing the Measuring head. Via an electrical interface of the beam lungsmeßgerätes the measuring head type is detected and the be appropriate measuring regimes set in the radiation measuring device.

Ein Ausführungsbeispiel der Erfindung ist in der Zeichnung dargestellt und wird im folgenden näher beschrieben.An embodiment of the invention is in the drawing shown and is described in more detail below.

Fig. 1 Strahlungsmeßgerät/Meßköpfe Fig. 1 radiation meter / measuring heads

Zwei Ausführungsbeispiele möglicher Meßköpfe, insbesondere für Lumineszenzmessung 5 und Fluoreszenzmessung 6 und das eigentliche Strahlungsmeßgerät 1 zeigt Fig. 1. An der Front­ seite des Strahlungsmeßgerätes 1 ist die Meßkopfaufnahme 2 mit der optischen 3 und elektrischen Kontaktvorrichtung 4 montiert. Das Strahlungsmeßgerät ist mit LCD-Anzeigetableau, Bedientastatur, Akkumulatorstromversorgung, Detektor und Con­ trollersteuerung als Handmeßgerät konzipiert. Eichfaktoren, Kennlinien und Steuerprogramm sind in einem nichtflüchtigen Speicher, Meßwerte und Zwischenergebnisse in einem Schreib-/ Lesespeicher abgelegt. Durch Detektion des Meßkopfes mittels elektrischer Schnittstelle 4 und Controllersteuerung wird das ablaufende Meßregime bestimmt. Hinter der optischen Kontakt­ vorrichtung 3 (Meßfenster) befindet sich der Strahlungsdetek­ tor.Two embodiments of possible measurement heads, in particular for luminescence 5 and 6 and the actual fluorescence measurement radiation measuring device 1 shown in FIG. 1. On the front side of the radiometer 1 is the Meßkopfaufnahme 2 3 with the optical and electrical contact device 4 is mounted. The radiation meter is designed as a hand-held meter with an LCD display panel, control keyboard, battery power supply, detector and controller control. Calibration factors, characteristic curves and control program are stored in a non-volatile memory, measured values and intermediate results in a read / write memory. Detecting the measuring head by means of electrical interface 4 and controller control determines the measuring regime that is taking place. Behind the optical contact device 3 (measuring window) is the radiation detector.

Der prinzipielle Aufbau der Meßköpfe 5 und 6 ist dadurch ge­ kennzeichnet, daß die Meßprobe unter der Abdeckung 7 dem Meß­ fenster 8 gegenübersteht und eine elektrische Schnittstelle 9 als Gegenstück zu der des Strahlungsmeßgerätes vorhanden ist. Die Koppel-(Front-)Seite der Meßköpfe 5, 6 ist so gestaltet, daß diese exakt strahlungsdicht an der Meßkopfaufnahme 2 des Strahlungsmeßgerätes 1 angesetzt werden können.The basic structure of the measuring heads 5 and 6 is characterized in that the sample under the cover 7 faces the measuring window 8 and an electrical interface 9 is available as a counterpart to that of the radiation measuring device. The coupling (front) side of the measuring heads 5 , 6 is designed in such a way that they can be attached exactly radiation-tight to the measuring head receptacle 2 of the radiation measuring device 1 .

Beide Meßköpfe 5, 6 unterscheiden sich im Wesentlichen nur da­ durch, daß der Fluoreszenzmeßkopf 6 zusätzlich eine monochro­ matische Lichtquelle hinter der Abdeckung 10 enthält. Die Stromversorgung der Lichtquelle erfolgt mittels der elek­ trischen Schnittstelle 9 bzw. 4 durch das Strahlungsmeßge­ rät. Zusatzeinrichtungen wie Filter, Optiken, Kühl- bzw. Temperiereinrichtungen sind bei Bedarf ebenfalls in den Meßköpfen integriert und werden gegebenenfalls über die elektrische Schnittstelle 9 vom Strahlungsmeßgerät 1 ver­ sorgt.The two measuring heads 5 , 6 differ essentially only in that the fluorescence measuring head 6 additionally contains a monochromatic light source behind the cover 10 . The power supply of the light source takes place by means of the elec trical interface 9 or 4 through the Radiation Meter. Additional equipment such as filters, lenses, cooling or temperature control are integrated if necessary also in the measurement heads and, if necessary, makes ver via the electrical interface 9 by the radiation measuring device. 1

Claims (1)

Strahlungsmeßgerät zur Lumineszenz- und Fluoreszenzmessung mit monochromatischer Lichtquelle, Detektoreinrichtung und control­ lergesteuertem Meßablauf in tragbarer Ausführung, dadurch gekennzeichnet, daß
  • a) mittels einer am Grundgerät montierten Meßkopfaufnahme mit optischer und elektrischer Schnittstelle Meßköpfe für unter­ schiedliche Strahlungsmessungen, insbesondere Fluoreszenz- und Lumineszenzmessungen, angekoppelt werden können,
  • b) für die unterschiedlichen Strahlungsmessungen erforderlichen Zusatzeinrichtungen, wie Filter, Optiken, Strahlungsquellen, Szintillatoren, Temperiereinrichtungen und Probeaufnahmen, in den Meßköpfen untergebracht sind,
  • c) durch Detektion mittels einer elektrischen Schnittstelle das Meßregime des Strahlungsmeßgerätes entsprechend dem eingesetz­ ten Meßkopf eingestellt wird.
Radiation measuring device for luminescence and fluorescence measurement with monochromatic light source, detector device and control-controlled measurement sequence in a portable design, characterized in that
  • a) measuring heads for different radiation measurements, in particular fluorescence and luminescence measurements, can be coupled by means of a measuring head mount with optical and electrical interface mounted on the basic device,
  • b) additional devices required for the different radiation measurements, such as filters, optics, radiation sources, scintillators, temperature control devices and test recordings, are accommodated in the measuring heads,
  • c) the measuring regime of the radiation measuring device is set in accordance with the inserted measuring head by detection by means of an electrical interface.
DE19934327752 1993-08-18 1993-08-18 Radiation measuring device for luminescence and fluorescence measurement Withdrawn DE4327752A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19934327752 DE4327752A1 (en) 1993-08-18 1993-08-18 Radiation measuring device for luminescence and fluorescence measurement

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19934327752 DE4327752A1 (en) 1993-08-18 1993-08-18 Radiation measuring device for luminescence and fluorescence measurement

Publications (1)

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DE4327752A1 true DE4327752A1 (en) 1995-02-23

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE29500705U1 (en) * 1995-01-18 1995-03-02 Future Technics Medizin Und Te Device for determining a certain proportion of gas in the air
DE19945392A1 (en) * 1999-09-22 2001-03-29 Robert Nusko Quantitative determination of ionic, non-ionic and amphoteric tensides and surface-active components involves using light source to excite dye and measuring change of fluorescence intensity
EP1291625A1 (en) * 2001-09-07 2003-03-12 Wallac Oy Advanced instrumentation for optical measurement of samples
WO2007092903A2 (en) 2006-02-08 2007-08-16 Beckman Coulter, Inc. Modular fluorimetric or photometric reader
DE202009012456U1 (en) 2009-09-12 2009-12-31 Sita Messtechnik Gmbh Device for measuring substance concentrations in solutions based on fluorescence measurement
US8496879B2 (en) 2006-02-08 2013-07-30 Molecular Devices, Llc Optical detection utilizing cartridge with tunable filter assembly
US8968658B2 (en) 2006-02-08 2015-03-03 Molecular Devices, Llc Luminescence measurement utilizing cartridge with integrated detector

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2517786A1 (en) * 1974-05-28 1975-12-11 Baxter Laboratories Inc PHOTOMETER
US4292527A (en) * 1978-10-06 1981-09-29 The United States Of America As Represented By The United States Department Of Energy Radiation detection system
US4618771A (en) * 1983-11-14 1986-10-21 Beckman Industrial Corporation Non-dispersive infrared analyzer having improved infrared source and detecting assemblies
US5061855A (en) * 1989-04-07 1991-10-29 Shin-Etsu Chemical Co., Ltd. Radiation detector
DE4222181A1 (en) * 1991-08-19 1993-02-25 Gen Atomics Assembly for converting spectrophotometer to fluorometer

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2517786A1 (en) * 1974-05-28 1975-12-11 Baxter Laboratories Inc PHOTOMETER
US4292527A (en) * 1978-10-06 1981-09-29 The United States Of America As Represented By The United States Department Of Energy Radiation detection system
US4618771A (en) * 1983-11-14 1986-10-21 Beckman Industrial Corporation Non-dispersive infrared analyzer having improved infrared source and detecting assemblies
US5061855A (en) * 1989-04-07 1991-10-29 Shin-Etsu Chemical Co., Ltd. Radiation detector
DE4222181A1 (en) * 1991-08-19 1993-02-25 Gen Atomics Assembly for converting spectrophotometer to fluorometer

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Masahiko *
NIWA, Shin-Ichiro: A portable diffuse reflectance spectrophotometer for rapid and automatic measurement of tissue. In: Meas. Sci. Technol. 4, 1993, S. 668-676 *
OSAWA *
Prospekt: Fluorometrie der Fa. Camag Chemie-Er- zeugnisse und Adsorptionstechnik AG, 4132 Muttenz/Schweiz, S. 1-25 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE29500705U1 (en) * 1995-01-18 1995-03-02 Future Technics Medizin Und Te Device for determining a certain proportion of gas in the air
DE19945392A1 (en) * 1999-09-22 2001-03-29 Robert Nusko Quantitative determination of ionic, non-ionic and amphoteric tensides and surface-active components involves using light source to excite dye and measuring change of fluorescence intensity
EP1291625A1 (en) * 2001-09-07 2003-03-12 Wallac Oy Advanced instrumentation for optical measurement of samples
US6822741B2 (en) 2001-09-07 2004-11-23 Wallac Oy Optical instrument and process for measurement of samples
JP2009526239A (en) * 2006-02-08 2009-07-16 ベックマン コールター， インコーポレイテッド Modular fluorescence and photometric reader
WO2007092903A3 (en) * 2006-02-08 2007-11-01 Beckman Coulter Inc Modular fluorimetric or photometric reader
WO2007092903A2 (en) 2006-02-08 2007-08-16 Beckman Coulter, Inc. Modular fluorimetric or photometric reader
US8119066B2 (en) 2006-02-08 2012-02-21 Molecular Devices, Llc Multimode reader
CN101379390B (en) * 2006-02-08 2013-07-10 贝克曼库尔特公司 Device, system and method for analyzing target in sample
US8496879B2 (en) 2006-02-08 2013-07-30 Molecular Devices, Llc Optical detection utilizing cartridge with tunable filter assembly
US8968658B2 (en) 2006-02-08 2015-03-03 Molecular Devices, Llc Luminescence measurement utilizing cartridge with integrated detector
DE202009012456U1 (en) 2009-09-12 2009-12-31 Sita Messtechnik Gmbh Device for measuring substance concentrations in solutions based on fluorescence measurement
EP2295953A1 (en) 2009-09-12 2011-03-16 SITA Messtechnik GmbH Device for measuring substance concentrations in solutions on the basis of a fluorescence measurement

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