DE3586889T2 - Regulatorregion fuer die expression von heterologen genen in hefe. - Google Patents

Regulatorregion fuer die expression von heterologen genen in hefe.

Info

Publication number
DE3586889T2
DE3586889T2 DE8585113737T DE3586889T DE3586889T2 DE 3586889 T2 DE3586889 T2 DE 3586889T2 DE 8585113737 T DE8585113737 T DE 8585113737T DE 3586889 T DE3586889 T DE 3586889T DE 3586889 T2 DE3586889 T2 DE 3586889T2
Authority
DE
Germany
Prior art keywords
yeast
expression
regulator region
heterological
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8585113737T
Other languages
English (en)
Other versions
DE3586889D1 (de
Inventor
David Womack Stroman
Paul Frederick Brust
Steven Bradley Ellis
Thomas Raymond Gingeras
Michael Miller Harpold
Juerg Friedrich Tschopp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RESEARCH CORPORATION TECHNOLOGIES, INC. (N.D.GES.D
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Application granted granted Critical
Publication of DE3586889D1 publication Critical patent/DE3586889D1/de
Publication of DE3586889T2 publication Critical patent/DE3586889T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/68Stabilisation of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
DE8585113737T 1984-10-30 1985-10-29 Regulatorregion fuer die expression von heterologen genen in hefe. Expired - Lifetime DE3586889T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast

Publications (2)

Publication Number Publication Date
DE3586889D1 DE3586889D1 (de) 1993-01-21
DE3586889T2 true DE3586889T2 (de) 1993-04-15

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585113737T Expired - Lifetime DE3586889T2 (de) 1984-10-30 1985-10-29 Regulatorregion fuer die expression von heterologen genen in hefe.

Country Status (23)

Country Link
US (1) US4855231A (de)
EP (2) EP0483115A3 (de)
JP (5) JP2502508B2 (de)
KR (1) KR930001117B1 (de)
CN (1) CN1011243B (de)
AR (1) AR242988A1 (de)
AT (1) ATE83263T1 (de)
AU (1) AU572002B2 (de)
CA (1) CA1340733C (de)
DD (1) DD254211A5 (de)
DE (1) DE3586889T2 (de)
DK (1) DK496385A (de)
ES (1) ES8609458A1 (de)
FI (1) FI94427C (de)
GR (1) GR852611B (de)
HU (1) HU205627B (de)
IE (1) IE930804L (de)
IL (1) IL76765A (de)
NO (1) NO178076C (de)
NZ (1) NZ213842A (de)
PL (1) PL158965B1 (de)
PT (1) PT81399B (de)
YU (1) YU46695B (de)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
EP0299108B1 (de) * 1987-07-17 1994-05-18 Rhein Biotech Gesellschaft für biotechnologische Prozesse und Produkte mbH DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung
US5179003A (en) * 1988-01-05 1993-01-12 Boehringer Mannheim Gmbh Process for the production of proteins or protein-containing gene products
SE8901264L (sv) * 1988-04-11 1989-10-12 Phillips Petroleum Co Uttryck av laxtillvaexthormon i metylotropisk jaest av slaektet pichia
IL89992A0 (en) 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89991A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of hepatitis b pres2 protein in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
ATE111524T1 (de) * 1988-12-20 1994-09-15 Rhein Biotech Ges Fuer Biotech Mutanter stamm von einem methylotrophen organismus und verfahren zur herstellung eines proteins in methylotrophen organismus.
DD297841A5 (de) * 1989-02-13 1992-01-23 The Salk Institute Biotechnology/Industrial Associates,Us Herstellung von superoxiddismutase in pichia pastoris hefezellen
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク エンド カンパニー インコーポレーテッド ピキア(Pichia)蛋白質分解活性に影響する遺伝子およびその使用
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
ATE141330T1 (de) * 1992-02-28 1996-08-15 Suntory Ltd Neuer vektor, einen von methanol und/oder glyzerin induzierbaren promotor enthaltend
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
JP3541949B2 (ja) * 1993-03-03 2004-07-14 ユニバーシテイ・オブ・アイオワ・リサーチ・フアウンデーシヨン メチロトローフ酵母におけるグリコレートオキシダーゼの製造
EP1508614A1 (de) * 1993-03-08 2005-02-23 MERCK & CO. INC. Menschliche neuronale Nikotin-acetylcholin-rezeptor-verbindungen und Methoden ihres Einsatzes
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
GB2286188B (en) 1993-04-20 1997-11-12 Salk Inst Biotech Ind Human N-methyl-D-aspartate receptor subunits,nucleic acids encoding same and uses therefor
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US5912122A (en) * 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US6001581A (en) 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US6638905B2 (en) * 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
GB2287941B (en) * 1993-11-08 1998-01-28 Salk Inst Biotech Ind Human alpha2 neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
JP3811186B2 (ja) * 1994-10-18 2006-08-16 コーバス インターナショナル, インコーポレイテッド 線虫から抽出されるセリンプロテアーゼ阻害剤および抗凝固性タンパク質
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
JP2000500015A (ja) * 1995-11-09 2000-01-11 ザイモジェネティクス,インコーポレイティド メチロトロープ性酵母におけるgad65の生成
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
EP0911416B1 (de) 1997-10-01 2006-05-17 DSM IP Assets B.V. Verfahren zur Protein-Herstellung
CN100342008C (zh) * 1997-10-24 2007-10-10 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
WO2000001731A1 (fr) * 1998-07-06 2000-01-13 Tosoh Corporation Proteine fusionnee avec liaison directe du recepteur il-6 a il-6
WO2000012687A1 (en) * 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
NZ514569A (en) 1999-03-02 2004-02-27 Invitrogen Corp Compositions and methods for use in recombinational cloning of nucleic acids
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
KR20020059719A (ko) 1999-11-12 2002-07-13 추후보정 재조합 젤라틴
CA2392753A1 (en) 1999-12-10 2001-06-14 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
EP2119793A1 (de) * 2000-06-28 2009-11-18 Glycofi, Inc. Verfahren zur Herstellung modifizierter Glykoproteine
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
EP2267135A3 (de) 2000-06-30 2011-09-14 Vib Vzw Modifikation der Proteinglycosylierung in Pichia Pastoris
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
MXPA03009059A (es) 2001-04-05 2004-10-15 Univ Nebraska Secuencias de nucleotidos reguladoras de alcohol oxidasa 1 para expresion de genes heterologos en levadura.
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
US20060088834A1 (en) * 2002-04-15 2006-04-27 Gretchen Bain Matrix analysis of gene expression in cells (magec)
IL165717A0 (en) 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
DE60319333D1 (de) * 2002-12-20 2008-04-10 Roche Diagnostics Gmbh Hitzelabile Desoxyribonuklease I-Varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (de) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Entglykosilierte Enzymen für Konjugaten
US7354734B2 (en) * 2003-08-25 2008-04-08 Funzyme Biotechnologies Sa Fungal proteins and nucleic acids encoding same
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
CA2486195C (en) * 2003-12-05 2012-03-06 Stephan Glaser Recombinantly expressed carboxypeptidase b and purification thereof
ES2370657T3 (es) * 2005-09-14 2011-12-21 Sanofi-Aventis Deutschland Gmbh Escisión de precursores de insulinas mediante una variante de tripsina.
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
AU2009223846B2 (en) * 2008-03-03 2014-11-20 Abbvie Inc. Methods for transforming yeast
KR20110101198A (ko) 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
EP2258855A1 (de) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expressionssequenzen
US20120276075A1 (en) 2009-12-21 2012-11-01 Centre Hospitalier Universitaire Vaudois (Chuv) Synergic action of a prolyl protease and tripeptidyl proteases
HRP20211873T1 (hr) 2011-05-20 2022-03-04 H. Lundbeck A/S Proizvodnja visoke čistoće proteina s više podjedinica kao što su protutijela u transformiranim mikrobima kao što je pichia pastoris
AU2012299035B2 (en) 2011-08-19 2017-11-02 H. Lundbeck A/S Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
ES2913825T3 (es) 2011-10-07 2022-06-06 Lonza Ag Promotor regulable
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
US10138294B2 (en) 2013-03-15 2018-11-27 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
AR095615A1 (es) 2013-03-15 2015-10-28 Alder Biopharmaceuticals Inc Purificación de anticuerpos y monitoreo de pureza
BR112015022558A2 (pt) 2013-03-15 2017-10-24 Lonza Ag sequência isolada de ácidos nucleicos do promotor pcs1, construção de expressão ou célula hospedeira recombinante compreendendo a sequência de ácidos nucleicos, cultura celular estável de uma pluralidade das referidas células hospedeiras, método para a produção de uma proteína de interesse e utilização de uma sequência isolada de ácidos nucleicos compreendendo um promotor em um método para a produção de uma proteína de interesse
AU2014234962C1 (en) 2013-03-21 2019-01-17 Evonik Operations Gmbh Purification of triple helical proteins
JP6440687B2 (ja) 2013-04-30 2018-12-19 ドナルド ダンフォース プラント サイエンス センター 抗真菌性植物タンパク質、ペプチド、及び使用方法
AU2014317807B2 (en) 2013-09-09 2019-02-21 Evonik Operations Gmbh Modified bacterial collagen-like proteins
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
WO1983004051A1 (en) * 1982-05-19 1983-11-24 Unilever N.V. Expression of preprothaumatin-like proteins in kluyveromyces yeasts
WO1984001153A1 (en) * 1982-09-15 1984-03-29 Collaborative Res Inc Production of interferon in yeast by use of invertase promoter
CA1341130C (en) * 1984-07-27 2000-10-31 Adrianus Marinus Ledeboer Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia

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JP2552808B2 (ja) 1996-11-13
ATE83263T1 (de) 1992-12-15
FI94427C (fi) 1995-09-11
PT81399A (en) 1985-11-01
EP0183071B1 (de) 1992-12-09
FI94427B (fi) 1995-05-31
CN1011243B (zh) 1991-01-16
JP2502508B2 (ja) 1996-05-29
DK496385D0 (da) 1985-10-29
ES8609458A1 (es) 1986-09-01
JPH06233686A (ja) 1994-08-23
FI854142L (fi) 1986-05-01
AU572002B2 (en) 1988-04-28
HUT38678A (en) 1986-06-30
JPH08196275A (ja) 1996-08-06
HU205627B (en) 1992-05-28
PL158965B1 (pl) 1992-10-30
NZ213842A (en) 1991-11-26
CA1340733C (en) 1999-09-14
NO178076B (no) 1995-10-09
KR930001117B1 (ko) 1993-02-18
EP0183071A2 (de) 1986-06-04
JPS61173781A (ja) 1986-08-05
AR242988A1 (es) 1993-06-30
NO178076C (no) 1996-01-17
PL256012A1 (en) 1986-09-23
DK496385A (da) 1986-05-01
EP0483115A2 (de) 1992-04-29
YU169585A (en) 1991-04-30
JPH0698789A (ja) 1994-04-12
AU4875285A (en) 1986-06-12
GR852611B (de) 1986-03-04
JPH08173161A (ja) 1996-07-09
DE3586889D1 (de) 1993-01-21
FI854142A0 (fi) 1985-10-23
EP0183071A3 (en) 1987-09-23
US4855231A (en) 1989-08-08
PT81399B (pt) 1987-11-11
IE930804L (en) 1986-04-30
JP2552807B2 (ja) 1996-11-13
NO854312L (no) 1986-05-02
DD254211A5 (de) 1988-02-17
CN85109718A (zh) 1986-08-20
IL76765A (en) 1991-06-30
KR870003197A (ko) 1987-04-15
EP0483115A3 (de) 1992-05-06
ES548307A0 (es) 1986-09-01
YU46695B (sh) 1994-04-05

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Free format text: BARDEHLE, H., DIPL.-ING. DOST, W., DIPL.-CHEM. DR.RER.NAT. ALTENBURG, U., DIPL.-PHYS., PAT.-ANWAELTEGEISSLER, B., DIPL.-PHYS.DR.JUR., PAT.- U. RECHTSANW. ROST, J., DIPL.-ING. DOSTERSCHILL, P., DIPL.- ING.DIPL.-WIRTSCH.-ING.DR.RER.POL., 81679 MUENCHEN KAHLHOEFER, H., DIPL.-PHYS., PAT.-ANWAELTE, 40474DUESSELDORF PAGENBERG, J., DR.JUR. FROHWITTER, B., DIPL.-ING., RECHTSANWAELTE, 81679 MUENCHEN

Free format text: BARDEHLE, H., DIPL.-ING. DOST, W., DIPL.-CHEM. DR.RER.NAT. ALTENBURG, U., DIPL.-PHYS., PAT.-ANWAELTE GEISSLER, B., DIPL.-PHYS.DR.JUR., PAT.- U. RECHTSANW. ROST, J., DIPL.-ING. DOSTERSCHILL, P., DIPL.-ING.DIPL.-WIRTSCH.-ING.DR.RER.POL., 81679 MUENCHEN KAHLHOEFER, H., DIPL.-PHYS., PAT.-ANWAELTE, 40474 DUESSELDORF PAGENBERG, J., DR.JUR. FROHWITTER, B., DIPL.-ING., RECHTSANWAELTE, 81679 MUENCHEN